avocadyne

heptadec-16-yne-1,2,4-triol

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2618
Screen concentration 43.4 μM
Source Microsource (Natural product library)
PubChem CID 3015189
SMILES C#CCCCCCCCCCCCC(CC(CO)O)O
Standardized SMILES OCC(O)CC(O)CCCCCCCCCCCC#C
Molecular weight 284.4342
ALogP 4.76
H-bond donor count 3
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 16.14
% growth inhibition (Hom. pool) 18.19


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3015189
Download HIP data (tab-delimited text)  (excel)
Gene:ARP3(YJR065C)|FD-Score:-3.24|P-value:6.04E-4|Clearance:0||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:BRF1(YGR246C)|FD-Score:3.48|P-value:2.50E-4|Clearance:0.14||SGD DESC:TFIIIB B-related factor, one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB Gene:CDC11(YJR076C)|FD-Score:3.18|P-value:7.42E-4|Clearance:0.07||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:ENP2(YGR145W)|FD-Score:3.1|P-value:9.67E-4|Clearance:0.11||SGD DESC:Essential nucleolar protein, required for biogenesis of the small ribosomal subunit; contains WD repeats, interacts with Mpp10p and Bfr2p, and has homology to Spb1p Gene:FAS1(YKL182W)|FD-Score:3.6|P-value:1.58E-4|Clearance:0.12||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:GCD11(YER025W)|FD-Score:-3.95|P-value:3.97E-5|Clearance:0||SGD DESC:Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met Gene:LIP1(YMR298W)|FD-Score:3.34|P-value:4.17E-4|Clearance:0.16||SGD DESC:Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis Gene:NAB3(YPL190C)|FD-Score:-3.36|P-value:3.87E-4|Clearance:0||SGD DESC:RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; required for termination of non-poly(A) transcripts and efficient splicing Gene:PAB1(YER165W)|FD-Score:-3.12|P-value:8.93E-4|Clearance:0||SGD DESC:Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G Gene:PIK1(YNL267W)|FD-Score:4.29|P-value:9.00E-6|Clearance:0.69||SGD DESC:Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton Gene:RPC53(YDL150W)|FD-Score:-3.29|P-value:5.02E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C53 Gene:RRN3(YKL125W)|FD-Score:4.74|P-value:1.08E-6|Clearance:0.69||SGD DESC:Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo Gene:WRS1(YOL097C)|FD-Score:4.73|P-value:1.14E-6|Clearance:0.69||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA Gene:YAH1(YPL252C)|FD-Score:3.11|P-value:9.38E-4|Clearance:0.01||SGD DESC:Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin Gene:ARP3(YJR065C)|FD-Score:-3.24|P-value:6.04E-4|Clearance:0||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:BRF1(YGR246C)|FD-Score:3.48|P-value:2.50E-4|Clearance:0.14||SGD DESC:TFIIIB B-related factor, one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB Gene:CDC11(YJR076C)|FD-Score:3.18|P-value:7.42E-4|Clearance:0.07||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:ENP2(YGR145W)|FD-Score:3.1|P-value:9.67E-4|Clearance:0.11||SGD DESC:Essential nucleolar protein, required for biogenesis of the small ribosomal subunit; contains WD repeats, interacts with Mpp10p and Bfr2p, and has homology to Spb1p Gene:FAS1(YKL182W)|FD-Score:3.6|P-value:1.58E-4|Clearance:0.12||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:GCD11(YER025W)|FD-Score:-3.95|P-value:3.97E-5|Clearance:0||SGD DESC:Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met Gene:LIP1(YMR298W)|FD-Score:3.34|P-value:4.17E-4|Clearance:0.16||SGD DESC:Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis Gene:NAB3(YPL190C)|FD-Score:-3.36|P-value:3.87E-4|Clearance:0||SGD DESC:RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; required for termination of non-poly(A) transcripts and efficient splicing Gene:PAB1(YER165W)|FD-Score:-3.12|P-value:8.93E-4|Clearance:0||SGD DESC:Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G Gene:PIK1(YNL267W)|FD-Score:4.29|P-value:9.00E-6|Clearance:0.69||SGD DESC:Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton Gene:RPC53(YDL150W)|FD-Score:-3.29|P-value:5.02E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C53 Gene:RRN3(YKL125W)|FD-Score:4.74|P-value:1.08E-6|Clearance:0.69||SGD DESC:Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo Gene:WRS1(YOL097C)|FD-Score:4.73|P-value:1.14E-6|Clearance:0.69||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA Gene:YAH1(YPL252C)|FD-Score:3.11|P-value:9.38E-4|Clearance:0.01||SGD DESC:Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3015189
Download HOP data (tab-delimited text)  (excel)
Gene:ADK1(YDR226W)|FD-Score:4.89|P-value:5.01E-7||SGD DESC:Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress Gene:AIM14(YGL160W)|FD-Score:3.8|P-value:7.36E-5||SGD DESC:NADPH oxidase localized to the perinuclear ER; produces superoxide from NADPH; overexpression causes MCA1 dependent apoptosis; likely involved in superoxide-mediated regulation of the actin cytoskeleton; member of a conserved superfamily of NADPH oxidases (NOX enzymes); has similarity to iron/copper reductases (FRE1-8), particularly Fre8p Gene:ARO1(YDR127W)|FD-Score:19.9|P-value:3.07E-88||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO2(YGL148W)|FD-Score:5.46|P-value:2.40E-8||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ARO7(YPR060C)|FD-Score:8.45|P-value:1.45E-17||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:ATP5(YDR298C)|FD-Score:-4.68|P-value:1.41E-6||SGD DESC:Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated Gene:BRE4(YDL231C)|FD-Score:3.72|P-value:9.79E-5||SGD DESC:Zinc finger protein containing five transmembrane domains; null mutant exhibits strongly fragmented vacuoles and sensitivity to brefeldin A, a drug which is known to affect intracellular transport Gene:CCW12(YLR110C)|FD-Score:3.79|P-value:7.52E-5||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CEM1(YER061C)|FD-Score:-3.2|P-value:6.78E-4||SGD DESC:Mitochondrial beta-keto-acyl synthase with possible role in fatty acid synthesis; required for mitochondrial respiration Gene:CHS5(YLR330W)|FD-Score:3.72|P-value:9.96E-5||SGD DESC:Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane Gene:CKA2(YOR061W)|FD-Score:23.4|P-value:6.57E-121||SGD DESC:Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching Gene:DAL81(YIR023W)|FD-Score:16|P-value:5.09E-58||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DOC1(YGL240W)|FD-Score:-3.41|P-value:3.25E-4||SGD DESC:Processivity factor required for the ubiquitination activity of the anaphase promoting complex (APC), mediates the activity of the APC by contributing to substrate recognition; involved in cyclin proteolysis; contains a conserved DOC1 homology domain Gene:EMC4(YGL231C)|FD-Score:3.29|P-value:5.06E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4, fly CG11137, human TMM85 Gene:ERG2(YMR202W)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERV14(YGL054C)|FD-Score:6.28|P-value:1.66E-10||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FET4(YMR319C)|FD-Score:3.88|P-value:5.25E-5||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FSH2(YMR222C)|FD-Score:4.12|P-value:1.88E-5||SGD DESC:Putative serine hydrolase that localizes to the cytoplasm; sequence is similar to S. cerevisiae Fsh1p and Fsh3p and the human candidate tumor suppressor OVCA2 Gene:GAL11(YOL051W)|FD-Score:6.02|P-value:8.90E-10||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GCN3(YKR026C)|FD-Score:6.06|P-value:6.67E-10||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:4.51|P-value:3.31E-6||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GPM3(YOL056W)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:HBN1(YCL026C-B_p)|FD-Score:3.16|P-value:7.80E-4||SGD DESC:Protein of unknown function; similar to bacterial nitroreductases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein becomes insoluble upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:HEK2(YBL032W)|FD-Score:7.8|P-value:3.02E-15||SGD DESC:RNA binding protein involved in the asymmetric localization of ASH1 mRNA; represses translation of ASH1 mRNA, an effect reversed by Yck1p-dependent phosphoryation; regulates telomere position effect and length; similarity to hnRNP-K Gene:HFI1(YPL254W)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions Gene:HNT2(YDR305C)|FD-Score:-3.32|P-value:4.46E-4||SGD DESC:Dinucleoside triphosphate hydrolase; has similarity to the tumor suppressor FHIT and belongs to the histidine triad (HIT) superfamily of nucleotide-binding proteins Gene:IGO1(YNL157W)|FD-Score:-3.26|P-value:5.53E-4||SGD DESC:Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; similar to Igo2p Gene:IMP2'(YIL154C)|FD-Score:5.4|P-value:3.39E-8||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:IRC25(YLR021W)|FD-Score:-3.43|P-value:3.02E-4||SGD DESC:Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions Gene:IRC7(YFR055W_p)|FD-Score:3.35|P-value:4.06E-4||SGD DESC:Beta-lyase involved in the production of thiols; null mutant displays increased levels of spontaneous Rad52p foci; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner and by copper levels in a Mac1-dependent manner Gene:IRS4(YKR019C)|FD-Score:-4.14|P-value:1.70E-5||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LAG1(YHL003C)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress Gene:MSH1(YHR120W)|FD-Score:-3.18|P-value:7.25E-4||SGD DESC:DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis Gene:NPP2(YEL016C)|FD-Score:3.27|P-value:5.30E-4||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp1p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP2 has a paralog, NPP1, that arose from the whole genome duplication Gene:OPI9(YLR338W_d)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:PCD1(YLR151C)|FD-Score:-3.33|P-value:4.40E-4||SGD DESC:Peroxisomal pyrophosphatase with specificity for coenzyme A and CoA derivatives, may function to remove potentially toxic oxidized CoA disulfide from peroxisomes to maintain the capacity for beta-oxidation of fatty acids; nudix hydrolase family member Gene:PFK26(YIL107C)|FD-Score:-3.89|P-value:4.97E-5||SGD DESC:6-phosphofructo-2-kinase, inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate; has negligible fructose-2,6-bisphosphatase activity; transcriptional regulation involves protein kinase A Gene:PGD1(YGL025C)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PGM3(YMR278W)|FD-Score:-3.41|P-value:3.25E-4||SGD DESC:Phosphoglucomutase, catalyzes interconversion of glucose-1-phosphate and glucose-6-phospate; transcription induced in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential Gene:PHO13(YDL236W)|FD-Score:-5.7|P-value:5.98E-9||SGD DESC:Alkaline phosphatase specific for p-nitrophenyl phosphate; also has protein phosphatase activity Gene:PRS3(YHL011C)|FD-Score:3.45|P-value:2.84E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:PTK2(YJR059W)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:PTR2(YKR093W)|FD-Score:3.12|P-value:9.16E-4||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:REG1(YDR028C)|FD-Score:5.14|P-value:1.41E-7||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RGA2(YDR379W)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:GTPase-activating protein for the polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; regulated by Pho85p and Cdc28p Gene:RGI2(YIL057C)|FD-Score:-3.37|P-value:3.77E-4||SGD DESC:Protein of unknown function involved in energy metabolism under respiratory conditions; expression induced under carbon limitation and repressed under high glucose Gene:RPN4(YDL020C)|FD-Score:-3.27|P-value:5.33E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS25A(YGR027C)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S25, no bacterial homolog; RPS25A has a paralog, RPS25B, that arose from the whole genome duplication Gene:SEH1(YGL100W)|FD-Score:-5.97|P-value:1.20E-9||SGD DESC:Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 Gene:SFB3(YHR098C)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SPF1(YEL031W)|FD-Score:3.74|P-value:9.12E-5||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SSN8(YNL025C)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Cyclin-like component of the RNA polymerase II holoenzyme, involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression and telomere maintenance Gene:SUR2(YDR297W)|FD-Score:34.1|P-value:2.83E-255||SGD DESC:Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis Gene:SUR4(YLR372W)|FD-Score:5.9|P-value:1.85E-9||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TAT1(YBR069C)|FD-Score:3.86|P-value:5.64E-5||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:THO2(YNL139C)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:Subunit of the THO complex, which is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids Gene:TRP1(YDR007W)|FD-Score:21.4|P-value:1.27E-101||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:12.4|P-value:2.08E-35||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:30.2|P-value:3.21E-201||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:28.3|P-value:2.01E-176||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:9.15|P-value:2.89E-20||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TRX1(YLR043C)|FD-Score:-3.9|P-value:4.89E-5||SGD DESC:Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p, required for ER-Golgi transport and vacuole inheritance; protein abundance increases in response to DNA replication stress Gene:TSC3(YBR058C-A)|FD-Score:9.35|P-value:4.27E-21||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TVP38(YKR088C)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; required for asymmetric localization of Kar9p during mitosis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:UBA4(YHR111W)|FD-Score:-3.2|P-value:6.77E-4||SGD DESC:Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p Gene:VPS63(YLR261C_d)|FD-Score:-4.59|P-value:2.20E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YBR285W(YBR285W_p)|FD-Score:3.24|P-value:6.03E-4||SGD DESC:Putative protein of unknown function; YBR285W is not an essential gene Gene:YCR050C(YCR050C_p)|FD-Score:3.12|P-value:9.15E-4||SGD DESC:Non-essential protein of unknown function; deletion mutant is synthetically sick or lethal with alpha-synuclein Gene:YDR008C(YDR008C_d)|FD-Score:15.6|P-value:1.90E-55||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR010C(YDR010C_d)|FD-Score:-3.28|P-value:5.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER156C(YER156C_p)|FD-Score:-4.63|P-value:1.79E-6||SGD DESC:Putative protein of unknown function; interacts with Hsp82p and copurifies with Ipl1p; expression is copper responsive and downregulated in strains deleted for MAC1, a copper-responsive transcription factor; similarity to mammalian MYG1 Gene:YGL117W(YGL117W_p)|FD-Score:5.76|P-value:4.33E-9||SGD DESC:Putative protein of unknown function Gene:YGR064W(YGR064W_d)|FD-Score:5.62|P-value:9.77E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YGR125W(YGR125W_p)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YHR202W(YHR202W_p)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization Gene:YJL135W(YJL135W_d)|FD-Score:5.19|P-value:1.05E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL134W/LCB3 Gene:YJL193W(YJL193W_p)|FD-Score:-3.37|P-value:3.76E-4||SGD DESC:Putative protein of unknown function, predicted to encode a triose phosphate transporter subfamily member based on phylogenetic analysis; similar to YOR307C/SLY41; deletion mutant has a respiratory growth defect Gene:YLR407W(YLR407W_p)|FD-Score:6.68|P-value:1.16E-11||SGD DESC:Putative protein of unknown function; null mutant displays elongated buds and a large fraction of budded cells have only one nucleus Gene:YML122C(YML122C_d)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL184C(YNL184C_p)|FD-Score:-3.3|P-value:4.90E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOR019W(YOR019W)|FD-Score:-3.61|P-value:1.50E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YOS9(YDR057W)|FD-Score:-3.55|P-value:1.92E-4||SGD DESC:ER quality-control lectin; integral subunit of the HRD ligase; binds to glycans with terminal alpha-1,6 linked mannose on misfolded N-glycosylated proteins and participates in targeting proteins to ERAD; member of the OS-9 protein family Gene:YPK1(YKL126W)|FD-Score:17.4|P-value:3.51E-68||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL068C(YPL068C_p)|FD-Score:-4.18|P-value:1.43E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YPR015C(YPR015C_p)|FD-Score:3.74|P-value:9.30E-5||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:ZWF1(YNL241C)|FD-Score:-3.27|P-value:5.40E-4||SGD DESC:Glucose-6-phosphate dehydrogenase (G6PD); catalyzes the first step of the pentose phosphate pathway; involved in adapting to oxidatve stress; homolog of the human G6PD which is deficient in patients with hemolytic anemia; protein abundance increases in response to DNA replication stress Gene:ADK1(YDR226W)|FD-Score:4.89|P-value:5.01E-7||SGD DESC:Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress Gene:AIM14(YGL160W)|FD-Score:3.8|P-value:7.36E-5||SGD DESC:NADPH oxidase localized to the perinuclear ER; produces superoxide from NADPH; overexpression causes MCA1 dependent apoptosis; likely involved in superoxide-mediated regulation of the actin cytoskeleton; member of a conserved superfamily of NADPH oxidases (NOX enzymes); has similarity to iron/copper reductases (FRE1-8), particularly Fre8p Gene:ARO1(YDR127W)|FD-Score:19.9|P-value:3.07E-88||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO2(YGL148W)|FD-Score:5.46|P-value:2.40E-8||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ARO7(YPR060C)|FD-Score:8.45|P-value:1.45E-17||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:ATP5(YDR298C)|FD-Score:-4.68|P-value:1.41E-6||SGD DESC:Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated Gene:BRE4(YDL231C)|FD-Score:3.72|P-value:9.79E-5||SGD DESC:Zinc finger protein containing five transmembrane domains; null mutant exhibits strongly fragmented vacuoles and sensitivity to brefeldin A, a drug which is known to affect intracellular transport Gene:CCW12(YLR110C)|FD-Score:3.79|P-value:7.52E-5||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CEM1(YER061C)|FD-Score:-3.2|P-value:6.78E-4||SGD DESC:Mitochondrial beta-keto-acyl synthase with possible role in fatty acid synthesis; required for mitochondrial respiration Gene:CHS5(YLR330W)|FD-Score:3.72|P-value:9.96E-5||SGD DESC:Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane Gene:CKA2(YOR061W)|FD-Score:23.4|P-value:6.57E-121||SGD DESC:Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching Gene:DAL81(YIR023W)|FD-Score:16|P-value:5.09E-58||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DOC1(YGL240W)|FD-Score:-3.41|P-value:3.25E-4||SGD DESC:Processivity factor required for the ubiquitination activity of the anaphase promoting complex (APC), mediates the activity of the APC by contributing to substrate recognition; involved in cyclin proteolysis; contains a conserved DOC1 homology domain Gene:EMC4(YGL231C)|FD-Score:3.29|P-value:5.06E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4, fly CG11137, human TMM85 Gene:ERG2(YMR202W)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERV14(YGL054C)|FD-Score:6.28|P-value:1.66E-10||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FET4(YMR319C)|FD-Score:3.88|P-value:5.25E-5||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FSH2(YMR222C)|FD-Score:4.12|P-value:1.88E-5||SGD DESC:Putative serine hydrolase that localizes to the cytoplasm; sequence is similar to S. cerevisiae Fsh1p and Fsh3p and the human candidate tumor suppressor OVCA2 Gene:GAL11(YOL051W)|FD-Score:6.02|P-value:8.90E-10||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GCN3(YKR026C)|FD-Score:6.06|P-value:6.67E-10||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:4.51|P-value:3.31E-6||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GPM3(YOL056W)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:HBN1(YCL026C-B_p)|FD-Score:3.16|P-value:7.80E-4||SGD DESC:Protein of unknown function; similar to bacterial nitroreductases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein becomes insoluble upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:HEK2(YBL032W)|FD-Score:7.8|P-value:3.02E-15||SGD DESC:RNA binding protein involved in the asymmetric localization of ASH1 mRNA; represses translation of ASH1 mRNA, an effect reversed by Yck1p-dependent phosphoryation; regulates telomere position effect and length; similarity to hnRNP-K Gene:HFI1(YPL254W)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions Gene:HNT2(YDR305C)|FD-Score:-3.32|P-value:4.46E-4||SGD DESC:Dinucleoside triphosphate hydrolase; has similarity to the tumor suppressor FHIT and belongs to the histidine triad (HIT) superfamily of nucleotide-binding proteins Gene:IGO1(YNL157W)|FD-Score:-3.26|P-value:5.53E-4||SGD DESC:Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; similar to Igo2p Gene:IMP2'(YIL154C)|FD-Score:5.4|P-value:3.39E-8||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:IRC25(YLR021W)|FD-Score:-3.43|P-value:3.02E-4||SGD DESC:Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions Gene:IRC7(YFR055W_p)|FD-Score:3.35|P-value:4.06E-4||SGD DESC:Beta-lyase involved in the production of thiols; null mutant displays increased levels of spontaneous Rad52p foci; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner and by copper levels in a Mac1-dependent manner Gene:IRS4(YKR019C)|FD-Score:-4.14|P-value:1.70E-5||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LAG1(YHL003C)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress Gene:MSH1(YHR120W)|FD-Score:-3.18|P-value:7.25E-4||SGD DESC:DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis Gene:NPP2(YEL016C)|FD-Score:3.27|P-value:5.30E-4||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp1p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP2 has a paralog, NPP1, that arose from the whole genome duplication Gene:OPI9(YLR338W_d)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:PCD1(YLR151C)|FD-Score:-3.33|P-value:4.40E-4||SGD DESC:Peroxisomal pyrophosphatase with specificity for coenzyme A and CoA derivatives, may function to remove potentially toxic oxidized CoA disulfide from peroxisomes to maintain the capacity for beta-oxidation of fatty acids; nudix hydrolase family member Gene:PFK26(YIL107C)|FD-Score:-3.89|P-value:4.97E-5||SGD DESC:6-phosphofructo-2-kinase, inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate; has negligible fructose-2,6-bisphosphatase activity; transcriptional regulation involves protein kinase A Gene:PGD1(YGL025C)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PGM3(YMR278W)|FD-Score:-3.41|P-value:3.25E-4||SGD DESC:Phosphoglucomutase, catalyzes interconversion of glucose-1-phosphate and glucose-6-phospate; transcription induced in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential Gene:PHO13(YDL236W)|FD-Score:-5.7|P-value:5.98E-9||SGD DESC:Alkaline phosphatase specific for p-nitrophenyl phosphate; also has protein phosphatase activity Gene:PRS3(YHL011C)|FD-Score:3.45|P-value:2.84E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:PTK2(YJR059W)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:PTR2(YKR093W)|FD-Score:3.12|P-value:9.16E-4||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:REG1(YDR028C)|FD-Score:5.14|P-value:1.41E-7||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RGA2(YDR379W)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:GTPase-activating protein for the polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; regulated by Pho85p and Cdc28p Gene:RGI2(YIL057C)|FD-Score:-3.37|P-value:3.77E-4||SGD DESC:Protein of unknown function involved in energy metabolism under respiratory conditions; expression induced under carbon limitation and repressed under high glucose Gene:RPN4(YDL020C)|FD-Score:-3.27|P-value:5.33E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS25A(YGR027C)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S25, no bacterial homolog; RPS25A has a paralog, RPS25B, that arose from the whole genome duplication Gene:SEH1(YGL100W)|FD-Score:-5.97|P-value:1.20E-9||SGD DESC:Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 Gene:SFB3(YHR098C)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SPF1(YEL031W)|FD-Score:3.74|P-value:9.12E-5||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SSN8(YNL025C)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Cyclin-like component of the RNA polymerase II holoenzyme, involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression and telomere maintenance Gene:SUR2(YDR297W)|FD-Score:34.1|P-value:2.83E-255||SGD DESC:Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis Gene:SUR4(YLR372W)|FD-Score:5.9|P-value:1.85E-9||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TAT1(YBR069C)|FD-Score:3.86|P-value:5.64E-5||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:THO2(YNL139C)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:Subunit of the THO complex, which is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids Gene:TRP1(YDR007W)|FD-Score:21.4|P-value:1.27E-101||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:12.4|P-value:2.08E-35||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:30.2|P-value:3.21E-201||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:28.3|P-value:2.01E-176||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:9.15|P-value:2.89E-20||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TRX1(YLR043C)|FD-Score:-3.9|P-value:4.89E-5||SGD DESC:Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p, required for ER-Golgi transport and vacuole inheritance; protein abundance increases in response to DNA replication stress Gene:TSC3(YBR058C-A)|FD-Score:9.35|P-value:4.27E-21||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TVP38(YKR088C)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; required for asymmetric localization of Kar9p during mitosis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:UBA4(YHR111W)|FD-Score:-3.2|P-value:6.77E-4||SGD DESC:Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p Gene:VPS63(YLR261C_d)|FD-Score:-4.59|P-value:2.20E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YBR285W(YBR285W_p)|FD-Score:3.24|P-value:6.03E-4||SGD DESC:Putative protein of unknown function; YBR285W is not an essential gene Gene:YCR050C(YCR050C_p)|FD-Score:3.12|P-value:9.15E-4||SGD DESC:Non-essential protein of unknown function; deletion mutant is synthetically sick or lethal with alpha-synuclein Gene:YDR008C(YDR008C_d)|FD-Score:15.6|P-value:1.90E-55||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR010C(YDR010C_d)|FD-Score:-3.28|P-value:5.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER156C(YER156C_p)|FD-Score:-4.63|P-value:1.79E-6||SGD DESC:Putative protein of unknown function; interacts with Hsp82p and copurifies with Ipl1p; expression is copper responsive and downregulated in strains deleted for MAC1, a copper-responsive transcription factor; similarity to mammalian MYG1 Gene:YGL117W(YGL117W_p)|FD-Score:5.76|P-value:4.33E-9||SGD DESC:Putative protein of unknown function Gene:YGR064W(YGR064W_d)|FD-Score:5.62|P-value:9.77E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YGR125W(YGR125W_p)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YHR202W(YHR202W_p)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization Gene:YJL135W(YJL135W_d)|FD-Score:5.19|P-value:1.05E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL134W/LCB3 Gene:YJL193W(YJL193W_p)|FD-Score:-3.37|P-value:3.76E-4||SGD DESC:Putative protein of unknown function, predicted to encode a triose phosphate transporter subfamily member based on phylogenetic analysis; similar to YOR307C/SLY41; deletion mutant has a respiratory growth defect Gene:YLR407W(YLR407W_p)|FD-Score:6.68|P-value:1.16E-11||SGD DESC:Putative protein of unknown function; null mutant displays elongated buds and a large fraction of budded cells have only one nucleus Gene:YML122C(YML122C_d)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL184C(YNL184C_p)|FD-Score:-3.3|P-value:4.90E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOR019W(YOR019W)|FD-Score:-3.61|P-value:1.50E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YOS9(YDR057W)|FD-Score:-3.55|P-value:1.92E-4||SGD DESC:ER quality-control lectin; integral subunit of the HRD ligase; binds to glycans with terminal alpha-1,6 linked mannose on misfolded N-glycosylated proteins and participates in targeting proteins to ERAD; member of the OS-9 protein family Gene:YPK1(YKL126W)|FD-Score:17.4|P-value:3.51E-68||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL068C(YPL068C_p)|FD-Score:-4.18|P-value:1.43E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YPR015C(YPR015C_p)|FD-Score:3.74|P-value:9.30E-5||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:ZWF1(YNL241C)|FD-Score:-3.27|P-value:5.40E-4||SGD DESC:Glucose-6-phosphate dehydrogenase (G6PD); catalyzes the first step of the pentose phosphate pathway; involved in adapting to oxidatve stress; homolog of the human G6PD which is deficient in patients with hemolytic anemia; protein abundance increases in response to DNA replication stress

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YKL125W4.741.08E-60.69RRN3Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo
YOL097C4.731.14E-60.69WRS1Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA
YNL267W4.299.00E-60.69PIK1Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton
YKL182W3.601.58E-40.12FAS1Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities
YGR246C3.482.50E-40.14BRF1TFIIIB B-related factor, one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB
YMR298W3.344.17E-40.16LIP1Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis
YJR076C3.187.42E-40.07CDC11Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YPL252C3.119.38E-40.01YAH1Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin
YGR145W3.109.67E-40.11ENP2Essential nucleolar protein, required for biogenesis of the small ribosomal subunit; contains WD repeats, interacts with Mpp10p and Bfr2p, and has homology to Spb1p
YDR050C2.990.001380.04TPI1Triose phosphate isomerase, abundant glycolytic enzyme; mRNA half-life is regulated by iron availability; transcription is controlled by activators Reb1p, Gcr1p, and Rap1p through binding sites in the 5' non-coding region; inhibition of Tpi1p activity by PEP (phosphoenolpyruvate) stimulates redox metabolism in respiring cells; E104D mutation in human TPI causes a rare autosomal disease
YNL062C2.950.001580.03GCD10Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression
YDL132W2.920.001730.02CDC53Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation
YMR185W_p2.900.001860.07RTP1_pProtein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene
YFR005C2.830.002300.05SAD1Conserved zinc-finger domain protein involved in pre-mRNA splicing, required for assembly of U4 snRNA into the U4/U6 particle
YGR075C2.780.002690.01PRP38Unique component of the U4/U6.U5 tri-snRNP particle, required for conformational changes which result in the catalytic activation of the spliceosome; dispensible for spliceosome assembly

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR297W34.102.83E-255SUR2Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis
YKL211C30.203.21E-201TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YDR354W28.302.01E-176TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YOR061W23.406.57E-121CKA2Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching
YDR007W21.401.27E-101TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YDR127W19.903.07E-88ARO1Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
YKL126W17.403.51E-68YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YIR023W16.005.09E-58DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YDR008C_d15.601.90E-55YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YER090W12.402.08E-35TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YBR058C-A9.354.27E-21TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YGL026C9.152.89E-20TRP5Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis
YPR060C8.451.45E-17ARO7Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis
YBL032W7.803.02E-15HEK2RNA binding protein involved in the asymmetric localization of ASH1 mRNA; represses translation of ASH1 mRNA, an effect reversed by Yck1p-dependent phosphoryation; regulates telomere position effect and length; similarity to hnRNP-K
YLR407W_p6.681.16E-11YLR407W_pPutative protein of unknown function; null mutant displays elongated buds and a large fraction of budded cells have only one nucleus

GO enrichment analysis for SGTC_2618
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.6190SGTC_2524avocatin b 10.6 μMMicrosource (Natural product library)67107480.515152
0.5340SGTC_2607solasodine 47.4 μMMicrosource (Natural product library)67106420.0123457
0.4990SGTC_505gw-5074 63.9 μMICCB bioactive library59242080.016129
0.4970SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.0930233
0.4890SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.0684932
0.4830SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.016129
0.4810SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.016129
0.4700SGTC_22547938975 95.2 μMChembridge (Fragment library)2969277NA
0.4621.54E-309SGTC_1824st053723 68.6 μMTimTec (Natural product derivative library)675225NA
0.4611.69E-308SGTC_1572epiandrosterone 68.9 μMTimTec (Pure natural product library)4413020.016129
0.4613.05E-308SGTC_20935331342 194.4 μMChembridge (Fragment library)338390.0357143
0.4582.57E-304SGTC_18825615643 20.0 μMMiscellaneous22530750.0135135TRP & mitochondrial translation
0.4573.70E-303SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.0714286
0.4572.69E-302SGTC_29399044578 71.4 μMChembridge (Drug-like library)26536340.0135135
0.4511.30E-293SGTC_23849036245 200.0 μMChembridge (Fragment library)64627660.0357143

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2502avocadyne acetate18.59 μM0.5526323952079Microsource (Natural product library)326.470865.13724
SGTC_2524avocatin b10.57 μM0.5151526710748Microsource (Natural product library)570.884248.94166
SGTC_2581avocatin a64 μM0.2826096710728Microsource (Natural product library)654.95769.69948sphingolipid biosynthesis & PDR1
SGTC_474dl-dihydrosphingosine5 μM0.2631583058739ICCB bioactive library301.507765.13433
SGTC_438sphingosine6.67 μM0.1777785280335ICCB bioactive library299.491884.82433
SGTC_436n,n-dimethylsphingosine3.3 μM0.1702135282309ICCB bioactive library327.545045.79223
SGTC_1616st00273456.1 μM0.1590914026101TimTec (Natural product derivative library)356.53997.40224
SGTC_456gingerol170 μM0.15442793ICCB bioactive library294.385943.63824
SGTC_3061glucosamine200 μM0.1591431Miscellaneous215.63206-2.69966
SGTC_470aa-861153 μM0.1458331967ICCB bioactive library326.429345.75713sphingolipid biosynthesis & PDR1