irigenin

5,7-dihydroxy-3-(3-hydroxy-4,5-dimethoxyphenyl)-6-methoxychromen-4-one

A phytoestrogen derived from Belamcanda chinensis with an antiproliferative effect on prostate cancer cells in vitro.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2620
Screen concentration 100.0 μM
Source Microsource (Natural product library)
PubChem CID 5464170
SMILES COC1=CC(=CC(=C1OC)O)C2=COC3=CC(=C(C(=C3C2=O)O)OC)O
Standardized SMILES COc1cc(cc(O)c1OC)C2=COc3cc(O)c(OC)c(O)c3C2=O
Molecular weight 360.3148
ALogP 2.09
H-bond donor count 3
H-bond acceptor count 8
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.64
% growth inhibition (Hom. pool) 6.67


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5464170
Download HIP data (tab-delimited text)  (excel)
Gene:AFG2(YLR397C)|FD-Score:3.21|P-value:6.62E-4|Clearance:0||SGD DESC:ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; may be involved in degradation of aberrant mRNAs Gene:ALG2(YGL065C)|FD-Score:3.69|P-value:1.12E-4|Clearance:0.02||SGD DESC:Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol Gene:BET1(YIL004C)|FD-Score:-3.28|P-value:5.26E-4|Clearance:0||SGD DESC:Type II membrane protein required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins Gene:BRF1(YGR246C)|FD-Score:3.67|P-value:1.23E-4|Clearance:0.1||SGD DESC:TFIIIB B-related factor, one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB Gene:CAK1(YFL029C)|FD-Score:4.56|P-value:2.61E-6|Clearance:0.15||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:CDC33(YOL139C)|FD-Score:5.63|P-value:8.99E-9|Clearance:0.75||SGD DESC:mRNA cap binding protein and translation initiation factor eIF4E; the eIF4E-cap complex is responsible for mediating cap-dependent mRNA translation via interactions with translation initiation factor eIF4G (Tif4631p or Tif4632p); protein abundance increases in response to DNA replication stress; mutants are defective for adhesion and pseudohyphal growth Gene:CDS1(YBR029C)|FD-Score:-3.47|P-value:2.57E-4|Clearance:0||SGD DESC:Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids Gene:CLP1(YOR250C)|FD-Score:4.68|P-value:1.46E-6|Clearance:0.05||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation Gene:CTF13(YMR094W)|FD-Score:3.99|P-value:3.33E-5|Clearance:0.03||SGD DESC:Subunit of the CBF3 complex, which binds to the CDE III element of centromeres, bending the DNA upon binding, and may be involved in sister chromatid cohesion during mitosis Gene:DIS3(YOL021C)|FD-Score:-5.36|P-value:4.17E-8|Clearance:0||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:EXO84(YBR102C)|FD-Score:4.25|P-value:1.08E-5|Clearance:0.21||SGD DESC:Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate Gene:GPI15(YNL038W)|FD-Score:4.04|P-value:2.69E-5|Clearance:0.05||SGD DESC:Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-H protein Gene:GPM1(YKL152C)|FD-Score:-3.57|P-value:1.80E-4|Clearance:0||SGD DESC:Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis Gene:HSH155(YMR288W)|FD-Score:3.95|P-value:3.85E-5|Clearance:0.11||SGD DESC:U2-snRNP associated splicing factor that forms extensive associations with the branch site-3' splice site-3' exon region upon prespliceosome formation; similarity to the mammalian U2 snRNP-associated splicing factor SAP155 Gene:KRE5(YOR336W)|FD-Score:4.88|P-value:5.18E-7|Clearance:0.02||SGD DESC:Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects Gene:MOB2(YFL034C-B)|FD-Score:-3.92|P-value:4.38E-5|Clearance:0||SGD DESC:Activator of Cbk1p kinase; component of the RAM signaling network that regulates cellular polarity and morphogenesis; activation of Cbk1p facilitates the Ace2p-dependent daughter cell-specific transcription of genes involved in cell separation; similar to Mob1p Gene:NDD1(YOR372C)|FD-Score:-3.9|P-value:4.82E-5|Clearance:0||SGD DESC:Transcriptional activator essential for nuclear division; localized to the nucleus; essential component of the mechanism that activates the expression of a set of late-S-phase-specific genes Gene:NOP7(YGR103W)|FD-Score:3.18|P-value:7.45E-4|Clearance:0.03||SGD DESC:Component of several different pre-ribosomal particles; forms a complex with Ytm1p and Erb1p that is required for maturation of the large ribosomal subunit; required for exit from G<sub>0</sub> and the initiation of cell proliferation Gene:NOP9(YJL010C)|FD-Score:4.63|P-value:1.87E-6|Clearance:0.07||SGD DESC:Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain Gene:ORC6(YHR118C)|FD-Score:4.4|P-value:5.31E-6|Clearance:0.11||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p Gene:PRP46(YPL151C)|FD-Score:9.83|P-value:4.19E-23|Clearance:1.87||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs Gene:RHO1(YPR165W)|FD-Score:8.31|P-value:4.72E-17|Clearance:1.87||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RIB5(YBR256C)|FD-Score:3.84|P-value:6.03E-5|Clearance:0.15||SGD DESC:Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway Gene:RPL17A(YKL180W)|FD-Score:-3.54|P-value:1.98E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPM2(YML091C)|FD-Score:-4.73|P-value:1.15E-6|Clearance:0||SGD DESC:Protein subunit of mitochondrial RNase P, has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucleus Gene:RPP1(YHR062C)|FD-Score:6.44|P-value:5.84E-11|Clearance:0.81||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRP15(YPR143W)|FD-Score:3.21|P-value:6.65E-4|Clearance:0.03||SGD DESC:Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs Gene:RTS2(YOR077W)|FD-Score:3.1|P-value:9.69E-4|Clearance:0.02||SGD DESC:Basic zinc-finger protein, similar to human and mouse Kin17 proteins which are chromatin-associated proteins involved in UV response and DNA replication Gene:SAM50(YNL026W)|FD-Score:-6.65|P-value:1.50E-11|Clearance:0||SGD DESC:Essential component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; homologous to bacterial Omp85 Gene:SCC4(YER147C)|FD-Score:-4.58|P-value:2.36E-6|Clearance:0||SGD DESC:Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for the loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during double-strand break repair via phosphorylated histone H2AX Gene:SEC53(YFL045C)|FD-Score:-4.23|P-value:1.18E-5|Clearance:0||SGD DESC:Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen Gene:SFH1(YLR321C)|FD-Score:-3.14|P-value:8.54E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:SRP54(YPR088C)|FD-Score:-4.44|P-value:4.40E-6|Clearance:0||SGD DESC:Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain Gene:SWD2(YKL018W)|FD-Score:4.86|P-value:5.78E-7|Clearance:0.19||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination Gene:TFB3(YDR460W)|FD-Score:3.7|P-value:1.09E-4|Clearance:0.01||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit Gene:TOM22(YNL131W)|FD-Score:-4.06|P-value:2.45E-5|Clearance:0||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:UTP6(YDR449C)|FD-Score:3.12|P-value:9.12E-4|Clearance:0.02||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:WRS1(YOL097C)|FD-Score:3.57|P-value:1.78E-4|Clearance:0.36||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA Gene:YJL009W(YJL009W_d)|FD-Score:-3.86|P-value:5.72E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex Gene:YJR012C(YJR012C_p)|FD-Score:4.3|P-value:8.69E-6|Clearance:0.05||SGD DESC:Essential protein of unknown function; proposed involvement in transport based on mass spectrometry analysis of copurifying proteins; partially overlaps neighboring ORF, GPI14/YJR013W Gene:YOR169C(YOR169C_d)|FD-Score:3.15|P-value:8.18E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GLN4/YOR168W Gene:AFG2(YLR397C)|FD-Score:3.21|P-value:6.62E-4|Clearance:0||SGD DESC:ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; may be involved in degradation of aberrant mRNAs Gene:ALG2(YGL065C)|FD-Score:3.69|P-value:1.12E-4|Clearance:0.02||SGD DESC:Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol Gene:BET1(YIL004C)|FD-Score:-3.28|P-value:5.26E-4|Clearance:0||SGD DESC:Type II membrane protein required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins Gene:BRF1(YGR246C)|FD-Score:3.67|P-value:1.23E-4|Clearance:0.1||SGD DESC:TFIIIB B-related factor, one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB Gene:CAK1(YFL029C)|FD-Score:4.56|P-value:2.61E-6|Clearance:0.15||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:CDC33(YOL139C)|FD-Score:5.63|P-value:8.99E-9|Clearance:0.75||SGD DESC:mRNA cap binding protein and translation initiation factor eIF4E; the eIF4E-cap complex is responsible for mediating cap-dependent mRNA translation via interactions with translation initiation factor eIF4G (Tif4631p or Tif4632p); protein abundance increases in response to DNA replication stress; mutants are defective for adhesion and pseudohyphal growth Gene:CDS1(YBR029C)|FD-Score:-3.47|P-value:2.57E-4|Clearance:0||SGD DESC:Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids Gene:CLP1(YOR250C)|FD-Score:4.68|P-value:1.46E-6|Clearance:0.05||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation Gene:CTF13(YMR094W)|FD-Score:3.99|P-value:3.33E-5|Clearance:0.03||SGD DESC:Subunit of the CBF3 complex, which binds to the CDE III element of centromeres, bending the DNA upon binding, and may be involved in sister chromatid cohesion during mitosis Gene:DIS3(YOL021C)|FD-Score:-5.36|P-value:4.17E-8|Clearance:0||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:EXO84(YBR102C)|FD-Score:4.25|P-value:1.08E-5|Clearance:0.21||SGD DESC:Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate Gene:GPI15(YNL038W)|FD-Score:4.04|P-value:2.69E-5|Clearance:0.05||SGD DESC:Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-H protein Gene:GPM1(YKL152C)|FD-Score:-3.57|P-value:1.80E-4|Clearance:0||SGD DESC:Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis Gene:HSH155(YMR288W)|FD-Score:3.95|P-value:3.85E-5|Clearance:0.11||SGD DESC:U2-snRNP associated splicing factor that forms extensive associations with the branch site-3' splice site-3' exon region upon prespliceosome formation; similarity to the mammalian U2 snRNP-associated splicing factor SAP155 Gene:KRE5(YOR336W)|FD-Score:4.88|P-value:5.18E-7|Clearance:0.02||SGD DESC:Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects Gene:MOB2(YFL034C-B)|FD-Score:-3.92|P-value:4.38E-5|Clearance:0||SGD DESC:Activator of Cbk1p kinase; component of the RAM signaling network that regulates cellular polarity and morphogenesis; activation of Cbk1p facilitates the Ace2p-dependent daughter cell-specific transcription of genes involved in cell separation; similar to Mob1p Gene:NDD1(YOR372C)|FD-Score:-3.9|P-value:4.82E-5|Clearance:0||SGD DESC:Transcriptional activator essential for nuclear division; localized to the nucleus; essential component of the mechanism that activates the expression of a set of late-S-phase-specific genes Gene:NOP7(YGR103W)|FD-Score:3.18|P-value:7.45E-4|Clearance:0.03||SGD DESC:Component of several different pre-ribosomal particles; forms a complex with Ytm1p and Erb1p that is required for maturation of the large ribosomal subunit; required for exit from G<sub>0</sub> and the initiation of cell proliferation Gene:NOP9(YJL010C)|FD-Score:4.63|P-value:1.87E-6|Clearance:0.07||SGD DESC:Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain Gene:ORC6(YHR118C)|FD-Score:4.4|P-value:5.31E-6|Clearance:0.11||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p Gene:PRP46(YPL151C)|FD-Score:9.83|P-value:4.19E-23|Clearance:1.87||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs Gene:RHO1(YPR165W)|FD-Score:8.31|P-value:4.72E-17|Clearance:1.87||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RIB5(YBR256C)|FD-Score:3.84|P-value:6.03E-5|Clearance:0.15||SGD DESC:Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway Gene:RPL17A(YKL180W)|FD-Score:-3.54|P-value:1.98E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPM2(YML091C)|FD-Score:-4.73|P-value:1.15E-6|Clearance:0||SGD DESC:Protein subunit of mitochondrial RNase P, has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucleus Gene:RPP1(YHR062C)|FD-Score:6.44|P-value:5.84E-11|Clearance:0.81||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRP15(YPR143W)|FD-Score:3.21|P-value:6.65E-4|Clearance:0.03||SGD DESC:Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs Gene:RTS2(YOR077W)|FD-Score:3.1|P-value:9.69E-4|Clearance:0.02||SGD DESC:Basic zinc-finger protein, similar to human and mouse Kin17 proteins which are chromatin-associated proteins involved in UV response and DNA replication Gene:SAM50(YNL026W)|FD-Score:-6.65|P-value:1.50E-11|Clearance:0||SGD DESC:Essential component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; homologous to bacterial Omp85 Gene:SCC4(YER147C)|FD-Score:-4.58|P-value:2.36E-6|Clearance:0||SGD DESC:Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for the loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during double-strand break repair via phosphorylated histone H2AX Gene:SEC53(YFL045C)|FD-Score:-4.23|P-value:1.18E-5|Clearance:0||SGD DESC:Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen Gene:SFH1(YLR321C)|FD-Score:-3.14|P-value:8.54E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:SRP54(YPR088C)|FD-Score:-4.44|P-value:4.40E-6|Clearance:0||SGD DESC:Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain Gene:SWD2(YKL018W)|FD-Score:4.86|P-value:5.78E-7|Clearance:0.19||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination Gene:TFB3(YDR460W)|FD-Score:3.7|P-value:1.09E-4|Clearance:0.01||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit Gene:TOM22(YNL131W)|FD-Score:-4.06|P-value:2.45E-5|Clearance:0||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:UTP6(YDR449C)|FD-Score:3.12|P-value:9.12E-4|Clearance:0.02||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:WRS1(YOL097C)|FD-Score:3.57|P-value:1.78E-4|Clearance:0.36||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA Gene:YJL009W(YJL009W_d)|FD-Score:-3.86|P-value:5.72E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex Gene:YJR012C(YJR012C_p)|FD-Score:4.3|P-value:8.69E-6|Clearance:0.05||SGD DESC:Essential protein of unknown function; proposed involvement in transport based on mass spectrometry analysis of copurifying proteins; partially overlaps neighboring ORF, GPI14/YJR013W Gene:YOR169C(YOR169C_d)|FD-Score:3.15|P-value:8.18E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GLN4/YOR168W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5464170
Download HOP data (tab-delimited text)  (excel)
Gene:ABP1(YCR088W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Actin-binding protein of the cortical actin cytoskeleton, important for activation of the Arp2/3 complex that plays a key role actin in cytoskeleton organization; phosphorylation within its PRR (Proline-Rich Region), mediated by Cdc28p and Pho85p, protects Abp1p from proteolysis mediated by its own PEST sequences Gene:ACH1(YBL015W)|FD-Score:-3.17|P-value:7.65E-4||SGD DESC:Protein with CoA transferase activity, particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth Gene:ADE12(YNL220W)|FD-Score:-18.5|P-value:4.85E-77||SGD DESC:Adenylosuccinate synthase, catalyzes the first step in synthesis of adenosine monophosphate from inosine 5'monophosphate during purine nucleotide biosynthesis; exhibits binding to single-stranded autonomously replicating (ARS) core sequence Gene:ADE17(YMR120C)|FD-Score:3.92|P-value:4.40E-5||SGD DESC:Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE17 has a paralog, ADE16, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine Gene:AEP2(YMR282C)|FD-Score:3.24|P-value:5.93E-4||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:AHC1(YOR023C)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Subunit of the Ada histone acetyltransferase complex, required for structural integrity of the complex Gene:ANT1(YPR128C)|FD-Score:-3.2|P-value:6.87E-4||SGD DESC:Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation Gene:ARO3(YDR035W)|FD-Score:4.1|P-value:2.07E-5||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ARO7(YPR060C)|FD-Score:-5.28|P-value:6.53E-8||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:ARO9(YHR137W)|FD-Score:4.92|P-value:4.41E-7||SGD DESC:Aromatic aminotransferase II, catalyzes the first step of tryptophan, phenylalanine, and tyrosine catabolism Gene:ASF1(YJL115W)|FD-Score:-4.43|P-value:4.74E-6||SGD DESC:Nucleosome assembly factor, involved in chromatin assembly and disassembly, anti-silencing protein that causes derepression of silent loci when overexpressed; plays a role in regulating Ty1 transposition Gene:BST1(YFL025C)|FD-Score:11.5|P-value:6.17E-31||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:BUD16(YEL029C)|FD-Score:-3.46|P-value:2.72E-4||SGD DESC:Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) Gene:CHD1(YER164W)|FD-Score:-3.28|P-value:5.16E-4||SGD DESC:Chromatin remodeler that regulates various aspects of transcription; acts in in conjunction with Isw1b to regulate chromatin structure and maintain chromatin integrity during transcription elongation by RNAP II by preventing trans-histone exchange over coding regions; contains a chromo domain, a helicase domain and a DNA-binding domain; component of both the SAGA and SLIK complexes Gene:CIR1(YGR207C)|FD-Score:4.72|P-value:1.20E-6||SGD DESC:Mitochondrial protein that interacts with frataxin (Yfh1p); putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-beta; may have a role in oxidative stress response Gene:CKA2(YOR061W)|FD-Score:3.97|P-value:3.61E-5||SGD DESC:Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching Gene:COX7(YMR256C)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CSF1(YLR087C)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CSM4(YPL200W)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:Protein required for accurate chromosome segregation during meiosis; involved in meiotic telomere clustering (bouquet formation) and telomere-led rapid prophase movements Gene:CTM1(YHR109W)|FD-Score:-4.05|P-value:2.61E-5||SGD DESC:Cytochrome c lysine methyltransferase, trimethylates residue 72 of apo-cytochrome c (Cyc1p) in the cytosol; not required for normal respiratory growth Gene:CYB2(YML054C)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Cytochrome b2 (L-lactate cytochrome-c oxidoreductase), component of the mitochondrial intermembrane space, required for lactate utilization; expression is repressed by glucose and anaerobic conditions Gene:DAL3(YIR032C)|FD-Score:4.02|P-value:2.91E-5||SGD DESC:Ureidoglycolate hydrolase, converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:DAL7(YIR031C)|FD-Score:3.15|P-value:8.05E-4||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DOT1(YDR440W)|FD-Score:4.3|P-value:8.70E-6||SGD DESC:Nucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response Gene:DOT6(YER088C)|FD-Score:3.93|P-value:4.31E-5||SGD DESC:Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; DOT6 has a paralog, TOD6, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ECM22(YLR228C)|FD-Score:5.39|P-value:3.55E-8||SGD DESC:Sterol regulatory element binding protein; regulates transcription of sterol biosynthetic genes; contains Zn[2]-Cys[6] binuclear cluster; relocates from intracellular membranes to perinuclear foci on sterol depletion; ECM22 has a paralog, UPC2, that arose from the whole genome duplication Gene:ECM29(YHL030W)|FD-Score:4.91|P-value:4.66E-7||SGD DESC:Scaffold protein; assists in association of the proteasome core particle with the regulatory particle; degraded by the mature proteasome after assembly; contains HEAT-like repeats; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:EOS1(YNL080C)|FD-Score:-4.53|P-value:2.94E-6||SGD DESC:Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene Gene:ERV14(YGL054C)|FD-Score:-3.14|P-value:8.36E-4||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:EXG2(YDR261C)|FD-Score:-3.4|P-value:3.37E-4||SGD DESC:Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:FAA3(YIL009W)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:Long chain fatty acyl-CoA synthetase, activates imported fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:FLO8(YER109C)|FD-Score:4.45|P-value:4.25E-6||SGD DESC:Transcription factor required for flocculation, diploid filamentous growth, and haploid invasive growth; genome reference strain S288C and most laboratory strains have a mutation in this gene Gene:FMP52(YER004W)|FD-Score:-4.13|P-value:1.79E-5||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FMT1(YBL013W)|FD-Score:-10.1|P-value:2.92E-24||SGD DESC:Methionyl-tRNA formyltransferase, catalyzes the formylation of initiator Met-tRNA in mitochondria; potential Cdc28p substrate Gene:GCN3(YKR026C)|FD-Score:3.83|P-value:6.53E-5||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GIR2(YDR152W)|FD-Score:4.66|P-value:1.55E-6||SGD DESC:Highly-acidic cytoplasmic RWD domain-containing protein of unknown function; forms a complex with Rbg2p; interacts with Rbg1p and Gcn1p; associates with translating ribosomes; putative intrinsically unstructured protein Gene:GIT1(YCR098C)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Plasma membrane permease, mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability Gene:GPB1(YOR371C)|FD-Score:4.82|P-value:7.34E-7||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; promotes ubiquitin-dependent proteolysis of Ira2p; regulated by G-alpha protein Gpa2p; GPB1 has a paralog, GPB2, that arose from the whole genome duplication Gene:GPM3(YOL056W)|FD-Score:3.73|P-value:9.63E-5||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:GYP1(YOR070C)|FD-Score:6.12|P-value:4.81E-10||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HOP2(YGL033W)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:ICS3(YJL077C)|FD-Score:5.06|P-value:2.12E-7||SGD DESC:Protein of unknown function; may play a role in vacuolar sorting, null mutants are hypersensitive to sortin2 Gene:IMG2(YCR071C)|FD-Score:5.39|P-value:3.61E-8||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:IRE1(YHR079C)|FD-Score:3.16|P-value:7.85E-4||SGD DESC:Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress Gene:ISC10(YER180C)|FD-Score:-5.3|P-value:5.67E-8||SGD DESC:Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells Gene:KHA1(YJL094C)|FD-Score:-3.47|P-value:2.61E-4||SGD DESC:Putative K+/H+ antiporter with a probable role in intracellular cation homeostasis, localized to Golgi vesicles and detected in highly purified mitochondria in high-throughput studies Gene:MAP1(YLR244C)|FD-Score:-3.21|P-value:6.68E-4||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p Gene:MCH1(YDL054C)|FD-Score:-3.19|P-value:7.03E-4||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MDR1(YGR100W)|FD-Score:3.21|P-value:6.55E-4||SGD DESC:Cytoplasmic GTPase-activating protein for Ypt/Rab transport GTPases Ypt6p, Ypt31p and Sec4p; involved in recycling of internalized proteins and regulation of Golgi secretory function Gene:MGA2(YIR033W)|FD-Score:-5.69|P-value:6.49E-9||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Spt23p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:MIC23(YBL107C_p)|FD-Score:-3.37|P-value:3.74E-4||SGD DESC:Mitochondrial intermembrane space protein of unknown function; imported via the MIA import machinery; contains an unusual twin cysteine motif (CX13C CX14C) Gene:MLH2(YLR035C)|FD-Score:4.17|P-value:1.54E-5||SGD DESC:Protein involved in the mismatch repair of certain frameshift intermediates and involved in meiotic recombination; forms a complex with Mlh1p Gene:MMS2(YGL087C)|FD-Score:4.55|P-value:2.65E-6||SGD DESC:Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress Gene:MNT4(YNR059W)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Putative alpha-1,3-mannosyltransferase, not required for protein O-glycosylation Gene:MRPL37(YBR268W)|FD-Score:-5.05|P-value:2.18E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRS4(YKR052C)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Iron transporter of the mitochondrial carrier family; mediates Fe2+ transport across the inner mitochondrial membrane; active under low-iron conditions; may transport other cations; protein abundance increases in response to DNA replication stress; MRS4 has a paralog, MRS3, that arose from the whole genome duplication Gene:NIT1(YIL164C)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Nitrilase, member of the nitrilase branch of the nitrilase superfamily; in closely related species and other S. cerevisiae strain backgrounds YIL164C and adjacent ORF, YIL165C, likely constitute a single ORF encoding a nitrilase gene Gene:NPR3(YHL023C)|FD-Score:-3.7|P-value:1.07E-4||SGD DESC:Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; required for Npr2p phosphorylation and Iml1p-Npr2p interaction; null mutant shows delayed meiotic DNA replication and double-strand break repair Gene:PCP1(YGR101W)|FD-Score:-6.27|P-value:1.80E-10||SGD DESC:Mitochondrial serine protease required for the processing of various mitochondrial proteins and maintenance of mitochondrial DNA and morphology; belongs to the rhomboid-GlpG superfamily of intramembrane peptidases Gene:PER1(YCR044C)|FD-Score:4.1|P-value:2.09E-5||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PRO2(YOR323C)|FD-Score:4.38|P-value:6.03E-6||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PRX1(YBL064C)|FD-Score:-3.95|P-value:3.92E-5||SGD DESC:Mitochondrial peroxiredoxin with thioredoxin peroxidase activity; has a role in reduction of hydroperoxides; reactivation requires Trr2p and glutathione; induced during respiratory growth and oxidative stress; phosphorylated; protein abundance increases in response to DNA replication stress Gene:RDL1(YOR285W)|FD-Score:3.18|P-value:7.30E-4||SGD DESC:Protein of unknown function containing a rhodanese-like domain; localized to the mitochondrial outer membrane; protein abundance increases in response to DNA replication stress Gene:REC8(YPR007C)|FD-Score:-3.57|P-value:1.82E-4||SGD DESC:Meiosis-specific component of sister chromatid cohesion complex; maintains cohesion between sister chromatids during meiosis I; maintains cohesion between centromeres of sister chromatids until meiosis II; homolog of S. pombe Rec8p Gene:RPA12(YJR063W)|FD-Score:-4.11|P-value:1.94E-5||SGD DESC:RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex Gene:RPL23A(YBL087C)|FD-Score:-9.74|P-value:9.82E-23||SGD DESC:Ribosomal 60S subunit protein L23A; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23A has a paralog, RPL23B, that arose from the whole genome duplication Gene:RPL33B(YOR234C)|FD-Score:-6.25|P-value:1.99E-10||SGD DESC:Ribosomal 60S subunit protein L33B; rpl33b null mutant exhibits normal growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33B has a paralog, RPL33A, that arose from the whole genome duplication Gene:RPS12(YOR369C)|FD-Score:-3.12|P-value:8.91E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S12, no bacterial homolog Gene:RRG8(YPR116W)|FD-Score:-3.35|P-value:4.00E-4||SGD DESC:Putative protein of unknown function, required for mitochondrial genome maintenance; null mutation results in a decrease in plasma membrane electron transport Gene:SAP4(YGL229C)|FD-Score:-5.13|P-value:1.48E-7||SGD DESC:Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p Gene:SCP1(YOR367W)|FD-Score:4.33|P-value:7.49E-6||SGD DESC:Component of yeast cortical actin cytoskeleton, binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin Gene:SLM4(YBR077C)|FD-Score:4.12|P-value:1.93E-5||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4 Gene:SLX1(YBR228W)|FD-Score:-3.29|P-value:5.01E-4||SGD DESC:Subunit of a complex, with Slx4p, that hydrolyzes 5' branches from duplex DNA in response to stalled or converging replication forks; function overlaps with that of Sgs1p-Top3p Gene:SLX9(YGR081C)|FD-Score:-3.81|P-value:7.08E-5||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SNC2(YOR327C)|FD-Score:-3.11|P-value:9.46E-4||SGD DESC:Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC2 has a paralog, SNC1, that arose from the whole genome duplication Gene:SPS4(YOR313C)|FD-Score:-3.1|P-value:9.70E-4||SGD DESC:Protein whose expression is induced during sporulation; not required for sporulation; heterologous expression in E. coli induces the SOS response that senses DNA damage Gene:SRO77(YBL106C)|FD-Score:4.18|P-value:1.44E-5||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:SSD1(YDR293C)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SSN2(YDR443C)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:SUR4(YLR372W)|FD-Score:3.85|P-value:5.98E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SWI6(YLR182W)|FD-Score:6.84|P-value:3.88E-12||SGD DESC:Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators Gene:SWS2(YNL081C)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:TOR1(YJR066W)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication Gene:TRP1(YDR007W)|FD-Score:8.74|P-value:1.17E-18||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:4.41|P-value:5.11E-6||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:11.9|P-value:3.83E-33||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:11.7|P-value:5.55E-32||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRS65(YGR166W)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response Gene:TSC3(YBR058C-A)|FD-Score:10.2|P-value:8.12E-25||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UAF30(YOR295W)|FD-Score:4.23|P-value:1.18E-5||SGD DESC:Subunit of UAF (upstream activation factor); UAF is an RNA polymerase I specific transcription stimulatory factor composed of Uaf30p, Rrn5p, Rrn9p, Rrn10p, histones H3 and H4; deletion decreases cellular growth rate; UAF30 has a paralog, TRI1, that arose from the whole genome duplication Gene:UBC11(YOR339C)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:Ubiquitin-conjugating enzyme most similar in sequence to Xenopus ubiquitin-conjugating enzyme E2-C, but not a true functional homolog of this E2; unlike E2-C, not required for the degradation of mitotic cyclin Clb2 Gene:VPH2(YKL119C)|FD-Score:-4.59|P-value:2.24E-6||SGD DESC:Integral membrane protein required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER) Gene:VPS51(YKR020W)|FD-Score:3.75|P-value:8.87E-5||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p Gene:VPS75(YNL246W)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress Gene:YBR196C-A(YBR196C-A_p)|FD-Score:-20.1|P-value:6.84E-90||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YCR075W-A(YCR075W-A_p)|FD-Score:-3.44|P-value:2.89E-4||SGD DESC:Putative protein of unknown function; identified by homology to Ashbya gossypii; YCR075W-A has a paralog, YNR034W-A, that arose from the whole genome duplication Gene:YDL118W(YDL118W_p)|FD-Score:-3.18|P-value:7.46E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YDR008C(YDR008C_d)|FD-Score:4.1|P-value:2.11E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR194W-A(YDR194W-A_p)|FD-Score:-3.32|P-value:4.52E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YEL043W(YEL043W)|FD-Score:4.2|P-value:1.31E-5||SGD DESC:Predicted cytoskeleton protein involved in intracellular signalling based on quantitative analysis of protein-protein interaction maps; may interact with ribosomes, based on co-purification studies; contains fibronectin type III domain fold Gene:YER119C-A(YER119C-A_d)|FD-Score:4.43|P-value:4.64E-6||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YER130C(YER130C_p)|FD-Score:-3.17|P-value:7.75E-4||SGD DESC:Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress Gene:YGL117W(YGL117W_p)|FD-Score:4.35|P-value:6.82E-6||SGD DESC:Putative protein of unknown function Gene:YGR169C-A(YGR169C-A_p)|FD-Score:3.25|P-value:5.79E-4||SGD DESC:Putative protein of unknown function; YGR169C-A has a paralog, YJR005C-A, that arose from the whole genome duplication Gene:YHP1(YDR451C)|FD-Score:3.95|P-value:3.94E-5||SGD DESC:Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication Gene:YHR080C(YHR080C)|FD-Score:4.41|P-value:5.16E-6||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YHR080C has a paralog, YSP2, that arose from the whole genome duplication Gene:YJL175W(YJL175W_d)|FD-Score:-4.87|P-value:5.66E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion confers resistance to cisplatin, hypersensitivity to 5-fluorouracil, and growth defect at high pH with high calcium; overlaps gene for SWI3 transcription factor Gene:YKL031W(YKL031W_d)|FD-Score:3.21|P-value:6.74E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species Gene:YKL063C(YKL063C_p)|FD-Score:4.08|P-value:2.29E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi Gene:YML018C(YML018C_p)|FD-Score:8.78|P-value:8.03E-19||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication Gene:YMR147W(YMR147W_p)|FD-Score:4.46|P-value:4.17E-6||SGD DESC:Putative protein of unknown function Gene:YNL195C(YNL195C_p)|FD-Score:-4.01|P-value:3.00E-5||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YNL198C(YNL198C_d)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL338W(YNL338W_d)|FD-Score:3.31|P-value:4.62E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps TEL14L-XC, which is Telomeric X element Core sequence on the left arm of Chromosome XIV Gene:YOR296W(YOR296W_p)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; expressed during copper starvation; YOR296W is not an essential gene Gene:YPK1(YKL126W)|FD-Score:19.6|P-value:4.74E-86||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPR170C(YPR170C_d)|FD-Score:-4.76|P-value:9.50E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B Gene:ZIP1(YDR285W)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Transverse filament protein of the synaptonemal complex; required for normal levels of meiotic recombination and pairing between homologous chromosome during meiosis; potential Cdc28p substrate Gene:ABP1(YCR088W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Actin-binding protein of the cortical actin cytoskeleton, important for activation of the Arp2/3 complex that plays a key role actin in cytoskeleton organization; phosphorylation within its PRR (Proline-Rich Region), mediated by Cdc28p and Pho85p, protects Abp1p from proteolysis mediated by its own PEST sequences Gene:ACH1(YBL015W)|FD-Score:-3.17|P-value:7.65E-4||SGD DESC:Protein with CoA transferase activity, particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth Gene:ADE12(YNL220W)|FD-Score:-18.5|P-value:4.85E-77||SGD DESC:Adenylosuccinate synthase, catalyzes the first step in synthesis of adenosine monophosphate from inosine 5'monophosphate during purine nucleotide biosynthesis; exhibits binding to single-stranded autonomously replicating (ARS) core sequence Gene:ADE17(YMR120C)|FD-Score:3.92|P-value:4.40E-5||SGD DESC:Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE17 has a paralog, ADE16, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine Gene:AEP2(YMR282C)|FD-Score:3.24|P-value:5.93E-4||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:AHC1(YOR023C)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Subunit of the Ada histone acetyltransferase complex, required for structural integrity of the complex Gene:ANT1(YPR128C)|FD-Score:-3.2|P-value:6.87E-4||SGD DESC:Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation Gene:ARO3(YDR035W)|FD-Score:4.1|P-value:2.07E-5||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ARO7(YPR060C)|FD-Score:-5.28|P-value:6.53E-8||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:ARO9(YHR137W)|FD-Score:4.92|P-value:4.41E-7||SGD DESC:Aromatic aminotransferase II, catalyzes the first step of tryptophan, phenylalanine, and tyrosine catabolism Gene:ASF1(YJL115W)|FD-Score:-4.43|P-value:4.74E-6||SGD DESC:Nucleosome assembly factor, involved in chromatin assembly and disassembly, anti-silencing protein that causes derepression of silent loci when overexpressed; plays a role in regulating Ty1 transposition Gene:BST1(YFL025C)|FD-Score:11.5|P-value:6.17E-31||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:BUD16(YEL029C)|FD-Score:-3.46|P-value:2.72E-4||SGD DESC:Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) Gene:CHD1(YER164W)|FD-Score:-3.28|P-value:5.16E-4||SGD DESC:Chromatin remodeler that regulates various aspects of transcription; acts in in conjunction with Isw1b to regulate chromatin structure and maintain chromatin integrity during transcription elongation by RNAP II by preventing trans-histone exchange over coding regions; contains a chromo domain, a helicase domain and a DNA-binding domain; component of both the SAGA and SLIK complexes Gene:CIR1(YGR207C)|FD-Score:4.72|P-value:1.20E-6||SGD DESC:Mitochondrial protein that interacts with frataxin (Yfh1p); putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-beta; may have a role in oxidative stress response Gene:CKA2(YOR061W)|FD-Score:3.97|P-value:3.61E-5||SGD DESC:Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching Gene:COX7(YMR256C)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CSF1(YLR087C)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CSM4(YPL200W)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:Protein required for accurate chromosome segregation during meiosis; involved in meiotic telomere clustering (bouquet formation) and telomere-led rapid prophase movements Gene:CTM1(YHR109W)|FD-Score:-4.05|P-value:2.61E-5||SGD DESC:Cytochrome c lysine methyltransferase, trimethylates residue 72 of apo-cytochrome c (Cyc1p) in the cytosol; not required for normal respiratory growth Gene:CYB2(YML054C)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Cytochrome b2 (L-lactate cytochrome-c oxidoreductase), component of the mitochondrial intermembrane space, required for lactate utilization; expression is repressed by glucose and anaerobic conditions Gene:DAL3(YIR032C)|FD-Score:4.02|P-value:2.91E-5||SGD DESC:Ureidoglycolate hydrolase, converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:DAL7(YIR031C)|FD-Score:3.15|P-value:8.05E-4||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DOT1(YDR440W)|FD-Score:4.3|P-value:8.70E-6||SGD DESC:Nucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response Gene:DOT6(YER088C)|FD-Score:3.93|P-value:4.31E-5||SGD DESC:Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; DOT6 has a paralog, TOD6, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ECM22(YLR228C)|FD-Score:5.39|P-value:3.55E-8||SGD DESC:Sterol regulatory element binding protein; regulates transcription of sterol biosynthetic genes; contains Zn[2]-Cys[6] binuclear cluster; relocates from intracellular membranes to perinuclear foci on sterol depletion; ECM22 has a paralog, UPC2, that arose from the whole genome duplication Gene:ECM29(YHL030W)|FD-Score:4.91|P-value:4.66E-7||SGD DESC:Scaffold protein; assists in association of the proteasome core particle with the regulatory particle; degraded by the mature proteasome after assembly; contains HEAT-like repeats; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:EOS1(YNL080C)|FD-Score:-4.53|P-value:2.94E-6||SGD DESC:Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene Gene:ERV14(YGL054C)|FD-Score:-3.14|P-value:8.36E-4||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:EXG2(YDR261C)|FD-Score:-3.4|P-value:3.37E-4||SGD DESC:Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:FAA3(YIL009W)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:Long chain fatty acyl-CoA synthetase, activates imported fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:FLO8(YER109C)|FD-Score:4.45|P-value:4.25E-6||SGD DESC:Transcription factor required for flocculation, diploid filamentous growth, and haploid invasive growth; genome reference strain S288C and most laboratory strains have a mutation in this gene Gene:FMP52(YER004W)|FD-Score:-4.13|P-value:1.79E-5||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FMT1(YBL013W)|FD-Score:-10.1|P-value:2.92E-24||SGD DESC:Methionyl-tRNA formyltransferase, catalyzes the formylation of initiator Met-tRNA in mitochondria; potential Cdc28p substrate Gene:GCN3(YKR026C)|FD-Score:3.83|P-value:6.53E-5||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GIR2(YDR152W)|FD-Score:4.66|P-value:1.55E-6||SGD DESC:Highly-acidic cytoplasmic RWD domain-containing protein of unknown function; forms a complex with Rbg2p; interacts with Rbg1p and Gcn1p; associates with translating ribosomes; putative intrinsically unstructured protein Gene:GIT1(YCR098C)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Plasma membrane permease, mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability Gene:GPB1(YOR371C)|FD-Score:4.82|P-value:7.34E-7||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; promotes ubiquitin-dependent proteolysis of Ira2p; regulated by G-alpha protein Gpa2p; GPB1 has a paralog, GPB2, that arose from the whole genome duplication Gene:GPM3(YOL056W)|FD-Score:3.73|P-value:9.63E-5||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:GYP1(YOR070C)|FD-Score:6.12|P-value:4.81E-10||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HOP2(YGL033W)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:ICS3(YJL077C)|FD-Score:5.06|P-value:2.12E-7||SGD DESC:Protein of unknown function; may play a role in vacuolar sorting, null mutants are hypersensitive to sortin2 Gene:IMG2(YCR071C)|FD-Score:5.39|P-value:3.61E-8||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:IRE1(YHR079C)|FD-Score:3.16|P-value:7.85E-4||SGD DESC:Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress Gene:ISC10(YER180C)|FD-Score:-5.3|P-value:5.67E-8||SGD DESC:Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells Gene:KHA1(YJL094C)|FD-Score:-3.47|P-value:2.61E-4||SGD DESC:Putative K+/H+ antiporter with a probable role in intracellular cation homeostasis, localized to Golgi vesicles and detected in highly purified mitochondria in high-throughput studies Gene:MAP1(YLR244C)|FD-Score:-3.21|P-value:6.68E-4||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p Gene:MCH1(YDL054C)|FD-Score:-3.19|P-value:7.03E-4||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MDR1(YGR100W)|FD-Score:3.21|P-value:6.55E-4||SGD DESC:Cytoplasmic GTPase-activating protein for Ypt/Rab transport GTPases Ypt6p, Ypt31p and Sec4p; involved in recycling of internalized proteins and regulation of Golgi secretory function Gene:MGA2(YIR033W)|FD-Score:-5.69|P-value:6.49E-9||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Spt23p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:MIC23(YBL107C_p)|FD-Score:-3.37|P-value:3.74E-4||SGD DESC:Mitochondrial intermembrane space protein of unknown function; imported via the MIA import machinery; contains an unusual twin cysteine motif (CX13C CX14C) Gene:MLH2(YLR035C)|FD-Score:4.17|P-value:1.54E-5||SGD DESC:Protein involved in the mismatch repair of certain frameshift intermediates and involved in meiotic recombination; forms a complex with Mlh1p Gene:MMS2(YGL087C)|FD-Score:4.55|P-value:2.65E-6||SGD DESC:Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress Gene:MNT4(YNR059W)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Putative alpha-1,3-mannosyltransferase, not required for protein O-glycosylation Gene:MRPL37(YBR268W)|FD-Score:-5.05|P-value:2.18E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRS4(YKR052C)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Iron transporter of the mitochondrial carrier family; mediates Fe2+ transport across the inner mitochondrial membrane; active under low-iron conditions; may transport other cations; protein abundance increases in response to DNA replication stress; MRS4 has a paralog, MRS3, that arose from the whole genome duplication Gene:NIT1(YIL164C)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Nitrilase, member of the nitrilase branch of the nitrilase superfamily; in closely related species and other S. cerevisiae strain backgrounds YIL164C and adjacent ORF, YIL165C, likely constitute a single ORF encoding a nitrilase gene Gene:NPR3(YHL023C)|FD-Score:-3.7|P-value:1.07E-4||SGD DESC:Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; required for Npr2p phosphorylation and Iml1p-Npr2p interaction; null mutant shows delayed meiotic DNA replication and double-strand break repair Gene:PCP1(YGR101W)|FD-Score:-6.27|P-value:1.80E-10||SGD DESC:Mitochondrial serine protease required for the processing of various mitochondrial proteins and maintenance of mitochondrial DNA and morphology; belongs to the rhomboid-GlpG superfamily of intramembrane peptidases Gene:PER1(YCR044C)|FD-Score:4.1|P-value:2.09E-5||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PRO2(YOR323C)|FD-Score:4.38|P-value:6.03E-6||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PRX1(YBL064C)|FD-Score:-3.95|P-value:3.92E-5||SGD DESC:Mitochondrial peroxiredoxin with thioredoxin peroxidase activity; has a role in reduction of hydroperoxides; reactivation requires Trr2p and glutathione; induced during respiratory growth and oxidative stress; phosphorylated; protein abundance increases in response to DNA replication stress Gene:RDL1(YOR285W)|FD-Score:3.18|P-value:7.30E-4||SGD DESC:Protein of unknown function containing a rhodanese-like domain; localized to the mitochondrial outer membrane; protein abundance increases in response to DNA replication stress Gene:REC8(YPR007C)|FD-Score:-3.57|P-value:1.82E-4||SGD DESC:Meiosis-specific component of sister chromatid cohesion complex; maintains cohesion between sister chromatids during meiosis I; maintains cohesion between centromeres of sister chromatids until meiosis II; homolog of S. pombe Rec8p Gene:RPA12(YJR063W)|FD-Score:-4.11|P-value:1.94E-5||SGD DESC:RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex Gene:RPL23A(YBL087C)|FD-Score:-9.74|P-value:9.82E-23||SGD DESC:Ribosomal 60S subunit protein L23A; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23A has a paralog, RPL23B, that arose from the whole genome duplication Gene:RPL33B(YOR234C)|FD-Score:-6.25|P-value:1.99E-10||SGD DESC:Ribosomal 60S subunit protein L33B; rpl33b null mutant exhibits normal growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33B has a paralog, RPL33A, that arose from the whole genome duplication Gene:RPS12(YOR369C)|FD-Score:-3.12|P-value:8.91E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S12, no bacterial homolog Gene:RRG8(YPR116W)|FD-Score:-3.35|P-value:4.00E-4||SGD DESC:Putative protein of unknown function, required for mitochondrial genome maintenance; null mutation results in a decrease in plasma membrane electron transport Gene:SAP4(YGL229C)|FD-Score:-5.13|P-value:1.48E-7||SGD DESC:Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p Gene:SCP1(YOR367W)|FD-Score:4.33|P-value:7.49E-6||SGD DESC:Component of yeast cortical actin cytoskeleton, binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin Gene:SLM4(YBR077C)|FD-Score:4.12|P-value:1.93E-5||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4 Gene:SLX1(YBR228W)|FD-Score:-3.29|P-value:5.01E-4||SGD DESC:Subunit of a complex, with Slx4p, that hydrolyzes 5' branches from duplex DNA in response to stalled or converging replication forks; function overlaps with that of Sgs1p-Top3p Gene:SLX9(YGR081C)|FD-Score:-3.81|P-value:7.08E-5||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SNC2(YOR327C)|FD-Score:-3.11|P-value:9.46E-4||SGD DESC:Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC2 has a paralog, SNC1, that arose from the whole genome duplication Gene:SPS4(YOR313C)|FD-Score:-3.1|P-value:9.70E-4||SGD DESC:Protein whose expression is induced during sporulation; not required for sporulation; heterologous expression in E. coli induces the SOS response that senses DNA damage Gene:SRO77(YBL106C)|FD-Score:4.18|P-value:1.44E-5||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:SSD1(YDR293C)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SSN2(YDR443C)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:SUR4(YLR372W)|FD-Score:3.85|P-value:5.98E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SWI6(YLR182W)|FD-Score:6.84|P-value:3.88E-12||SGD DESC:Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators Gene:SWS2(YNL081C)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:TOR1(YJR066W)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication Gene:TRP1(YDR007W)|FD-Score:8.74|P-value:1.17E-18||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:4.41|P-value:5.11E-6||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:11.9|P-value:3.83E-33||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:11.7|P-value:5.55E-32||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRS65(YGR166W)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response Gene:TSC3(YBR058C-A)|FD-Score:10.2|P-value:8.12E-25||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UAF30(YOR295W)|FD-Score:4.23|P-value:1.18E-5||SGD DESC:Subunit of UAF (upstream activation factor); UAF is an RNA polymerase I specific transcription stimulatory factor composed of Uaf30p, Rrn5p, Rrn9p, Rrn10p, histones H3 and H4; deletion decreases cellular growth rate; UAF30 has a paralog, TRI1, that arose from the whole genome duplication Gene:UBC11(YOR339C)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:Ubiquitin-conjugating enzyme most similar in sequence to Xenopus ubiquitin-conjugating enzyme E2-C, but not a true functional homolog of this E2; unlike E2-C, not required for the degradation of mitotic cyclin Clb2 Gene:VPH2(YKL119C)|FD-Score:-4.59|P-value:2.24E-6||SGD DESC:Integral membrane protein required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER) Gene:VPS51(YKR020W)|FD-Score:3.75|P-value:8.87E-5||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p Gene:VPS75(YNL246W)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress Gene:YBR196C-A(YBR196C-A_p)|FD-Score:-20.1|P-value:6.84E-90||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YCR075W-A(YCR075W-A_p)|FD-Score:-3.44|P-value:2.89E-4||SGD DESC:Putative protein of unknown function; identified by homology to Ashbya gossypii; YCR075W-A has a paralog, YNR034W-A, that arose from the whole genome duplication Gene:YDL118W(YDL118W_p)|FD-Score:-3.18|P-value:7.46E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein Gene:YDR008C(YDR008C_d)|FD-Score:4.1|P-value:2.11E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR194W-A(YDR194W-A_p)|FD-Score:-3.32|P-value:4.52E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YEL043W(YEL043W)|FD-Score:4.2|P-value:1.31E-5||SGD DESC:Predicted cytoskeleton protein involved in intracellular signalling based on quantitative analysis of protein-protein interaction maps; may interact with ribosomes, based on co-purification studies; contains fibronectin type III domain fold Gene:YER119C-A(YER119C-A_d)|FD-Score:4.43|P-value:4.64E-6||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YER130C(YER130C_p)|FD-Score:-3.17|P-value:7.75E-4||SGD DESC:Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress Gene:YGL117W(YGL117W_p)|FD-Score:4.35|P-value:6.82E-6||SGD DESC:Putative protein of unknown function Gene:YGR169C-A(YGR169C-A_p)|FD-Score:3.25|P-value:5.79E-4||SGD DESC:Putative protein of unknown function; YGR169C-A has a paralog, YJR005C-A, that arose from the whole genome duplication Gene:YHP1(YDR451C)|FD-Score:3.95|P-value:3.94E-5||SGD DESC:Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication Gene:YHR080C(YHR080C)|FD-Score:4.41|P-value:5.16E-6||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YHR080C has a paralog, YSP2, that arose from the whole genome duplication Gene:YJL175W(YJL175W_d)|FD-Score:-4.87|P-value:5.66E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion confers resistance to cisplatin, hypersensitivity to 5-fluorouracil, and growth defect at high pH with high calcium; overlaps gene for SWI3 transcription factor Gene:YKL031W(YKL031W_d)|FD-Score:3.21|P-value:6.74E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species Gene:YKL063C(YKL063C_p)|FD-Score:4.08|P-value:2.29E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi Gene:YML018C(YML018C_p)|FD-Score:8.78|P-value:8.03E-19||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication Gene:YMR147W(YMR147W_p)|FD-Score:4.46|P-value:4.17E-6||SGD DESC:Putative protein of unknown function Gene:YNL195C(YNL195C_p)|FD-Score:-4.01|P-value:3.00E-5||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YNL198C(YNL198C_d)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL338W(YNL338W_d)|FD-Score:3.31|P-value:4.62E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps TEL14L-XC, which is Telomeric X element Core sequence on the left arm of Chromosome XIV Gene:YOR296W(YOR296W_p)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; expressed during copper starvation; YOR296W is not an essential gene Gene:YPK1(YKL126W)|FD-Score:19.6|P-value:4.74E-86||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPR170C(YPR170C_d)|FD-Score:-4.76|P-value:9.50E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B Gene:ZIP1(YDR285W)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Transverse filament protein of the synaptonemal complex; required for normal levels of meiotic recombination and pairing between homologous chromosome during meiosis; potential Cdc28p substrate

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPL151C9.834.19E-231.87PRP46Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs
YPR165W8.314.72E-171.87RHO1GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
YHR062C6.445.84E-110.81RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YOL139C5.638.99E-90.75CDC33mRNA cap binding protein and translation initiation factor eIF4E; the eIF4E-cap complex is responsible for mediating cap-dependent mRNA translation via interactions with translation initiation factor eIF4G (Tif4631p or Tif4632p); protein abundance increases in response to DNA replication stress; mutants are defective for adhesion and pseudohyphal growth
YOR336W4.885.18E-70.02KRE5Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects
YKL018W4.865.78E-70.19SWD2Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination
YOR250C4.681.46E-60.05CLP1Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation
YJL010C4.631.87E-60.07NOP9Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain
YFL029C4.562.61E-60.15CAK1Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases
YHR118C4.405.31E-60.11ORC6Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p
YJR012C_p4.308.69E-60.05YJR012C_pEssential protein of unknown function; proposed involvement in transport based on mass spectrometry analysis of copurifying proteins; partially overlaps neighboring ORF, GPI14/YJR013W
YBR102C4.251.08E-50.21EXO84Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate
YNL038W4.042.69E-50.05GPI15Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-H protein
YMR094W3.993.33E-50.03CTF13Subunit of the CBF3 complex, which binds to the CDE III element of centromeres, bending the DNA upon binding, and may be involved in sister chromatid cohesion during mitosis
YMR288W3.953.85E-50.11HSH155U2-snRNP associated splicing factor that forms extensive associations with the branch site-3' splice site-3' exon region upon prespliceosome formation; similarity to the mammalian U2 snRNP-associated splicing factor SAP155

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKL126W19.604.74E-86YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YKL211C11.903.83E-33TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YDR354W11.705.55E-32TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YFL025C11.506.17E-31BST1GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules
YBR058C-A10.208.12E-25TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YML018C_p8.788.03E-19YML018C_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication
YDR007W8.741.17E-18TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YLR182W6.843.88E-12SWI6Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators
YOR070C6.124.81E-10GYP1Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion
YLR228C5.393.55E-8ECM22Sterol regulatory element binding protein; regulates transcription of sterol biosynthetic genes; contains Zn[2]-Cys[6] binuclear cluster; relocates from intracellular membranes to perinuclear foci on sterol depletion; ECM22 has a paralog, UPC2, that arose from the whole genome duplication
YCR071C5.393.61E-8IMG2Mitochondrial ribosomal protein of the large subunit
YJL077C5.062.12E-7ICS3Protein of unknown function; may play a role in vacuolar sorting, null mutants are hypersensitive to sortin2
YHR137W4.924.41E-7ARO9Aromatic aminotransferase II, catalyzes the first step of tryptophan, phenylalanine, and tyrosine catabolism
YHL030W4.914.66E-7ECM29Scaffold protein; assists in association of the proteasome core particle with the regulatory particle; degraded by the mature proteasome after assembly; contains HEAT-like repeats; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress
YOR371C4.827.34E-7GPB1Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; promotes ubiquitin-dependent proteolysis of Ira2p; regulated by G-alpha protein Gpa2p; GPB1 has a paralog, GPB2, that arose from the whole genome duplication

GO enrichment analysis for SGTC_2620
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2771.74E-104SGTC_2618avocadyne 43.4 μMMicrosource (Natural product library)30151890.015625
0.2611.83E-92SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.057971TSC3-RPN4
0.2549.41E-88SGTC_305-(2-thienylidene)-Rhodanine 28.7 μMChemDiv (Drug-like library)12411320.0588235
0.2483.54E-83SGTC_2524avocatin b 10.6 μMMicrosource (Natural product library)67107480.015625
0.2466.09E-82SGTC_1464k208-0027 110.0 μMChemDiv (Drug-like library)X14640.0434783
0.2459.25E-82SGTC_29399044578 71.4 μMChembridge (Drug-like library)26536340.125
0.2403.33E-78SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.0508475
0.2131.26E-61SGTC_14113909-8223 150.0 μMChemDiv (Drug-like library)X14110.021978
0.2091.63E-59SGTC_14964469-0713 57.4 μMChemDiv (Drug-like library)7466910.0769231
0.2036.84E-56SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.0833333
0.2023.30E-55SGTC_15465-methoxyflavanone 78.7 μMTimTec (Pure natural product library)1477950.206349
0.1981.83E-53SGTC_2669eugenol 100.0 μMMicrosource (Natural product library)33140.214286
0.1951.46E-51SGTC_1801486-1293 102.8 μMChemDiv (Drug-like library)41405360.060241
0.1943.81E-51SGTC_9262268-1401 59.4 μMChemDiv (Drug-like library)29315780.0506329TSC3-RPN4
0.1931.77E-50SGTC_2627acetyl isogambogic acid 100.0 μMMicrosource (Natural product library)68577890.0990991TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2522biochanin a4.94 μM0.4716985280373Microsource (Natural product library)284.263482.36625RNA pol III & RNase P/MRP
SGTC_1929genistein trimethyl ether50.5 μM0.461538136420TimTec (Natural product derivative library)312.316642.81705
SGTC_2557derrustone83.84 μM0.40678629853Microsource (Natural product library)326.300162.60106
SGTC_26335,7-dihydroxyisoflavone100 μM0.4038465377381Microsource (Natural product library)254.23752.38224
SGTC_1965Pectolinarigenin58.4 μM0.4035095320438TimTec (Natural product derivative library)314.289462.61926RPP1 & pyrimidine depletion
SGTC_2411st07713339.94 μM0.3833331403142TimTec (Natural product derivative library)368.336842.38507RNA pol III & RNase P/MRP
SGTC_1590st07710857.8 μM0.3728815489485TimTec (Pure natural product library)346.288261.83948
SGTC_1123mangostin811.29 nM0.3606565281650TimTec (Natural product library)410.459645.93536mitochondrial response to ROS
SGTC_2595hexamethylquercetagetin100 μM0.338983386331Microsource (Natural product library)402.394582.74208
SGTC_2596sinensetin100 μM0.322034145659Microsource (Natural product library)372.36863.05407