lithochol-11-enic acid

4-[(3R,5R,10S,13R,17R)-3-hydroxy-10,13-dimethyl-2,3,4,5,6,7,8,9,14,15,16,17-dodecahydro-1H-cyclopenta[a]phenanthren-17-yl]pentanoic acid

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2621
Screen concentration 100.0 μM
Source Microsource (Natural product library)
PubChem CID 6708711
SMILES CC(CCC(=O)O)C1CCC2C1(C=CC3C2CCC4C3(CCC(C4)O)C)C
Standardized SMILES CC(CCC(=O)O)C1CCC2C3CCC4CC(O)CCC4(C)C3C=CC12C
Molecular weight 374.5567
ALogP 4.74
H-bond donor count 2
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 5.98
% growth inhibition (Hom. pool) 6.67


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6708711
Download HIP data (tab-delimited text)  (excel)
Gene:ACT1(YFL039C)|FD-Score:4.51|P-value:3.27E-6|Clearance:0.63||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:ALA1(YOR335C)|FD-Score:3.27|P-value:5.44E-4|Clearance:0.1||SGD DESC:Cytoplasmic and mitochondrial alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog Gene:BET2(YPR176C)|FD-Score:3.52|P-value:2.18E-4|Clearance:0.04||SGD DESC:Beta subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:BET4(YJL031C)|FD-Score:-3.12|P-value:8.97E-4|Clearance:0||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:BRN1(YBL097W)|FD-Score:3.82|P-value:6.79E-5|Clearance:0.27||SGD DESC:Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission Gene:CDC16(YKL022C)|FD-Score:3.13|P-value:8.75E-4|Clearance:0.02||SGD DESC:Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation Gene:CDC48(YDL126C)|FD-Score:-3.12|P-value:9.08E-4|Clearance:0||SGD DESC:AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP Gene:CFD1(YIL003W)|FD-Score:-3.14|P-value:8.42E-4|Clearance:0||SGD DESC:Highly conserved, iron-sulfur cluster binding protein localized in the cytoplasm; forms a complex with Nbp35p that is involved in iron-sulfur protein assembly in the cytosol Gene:COP1(YDL145C)|FD-Score:3.48|P-value:2.54E-4|Clearance:0.01||SGD DESC:Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway Gene:DML1(YMR211W)|FD-Score:3.47|P-value:2.62E-4|Clearance:0.03||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:ERG8(YMR220W)|FD-Score:4.84|P-value:6.65E-7|Clearance:0.33||SGD DESC:Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:FAD1(YDL045C)|FD-Score:6.08|P-value:6.04E-10|Clearance:0.91||SGD DESC:Flavin adenine dinucleotide (FAD) synthetase, performs the second step in synthesis of FAD from riboflavin Gene:FAS1(YKL182W)|FD-Score:3.87|P-value:5.37E-5|Clearance:0||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:IPI1(YHR085W)|FD-Score:-4.66|P-value:1.61E-6|Clearance:0||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; IPI1 is an essential gene Gene:MED6(YHR058C)|FD-Score:-6.29|P-value:1.56E-10|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress Gene:MVD1(YNR043W)|FD-Score:-4.5|P-value:3.46E-6|Clearance:0||SGD DESC:Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer Gene:NSE3(YDR288W)|FD-Score:3.17|P-value:7.68E-4|Clearance:0.04||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress Gene:PHS1(YJL097W)|FD-Score:3.87|P-value:5.46E-5|Clearance:0.05||SGD DESC:Essential 3-hydroxyacyl-CoA dehydratase of the ER membrane, involved in elongation of very long-chain fatty acids; evolutionarily conserved, similar to mammalian PTPLA and PTPLB; involved in sphingolipid biosynthesis and protein trafficking Gene:RCL1(YOL010W)|FD-Score:3.44|P-value:2.89E-4|Clearance:0.17||SGD DESC:Endonuclease that cleaves pre-rRNA at site A2 for 18S rRNA biogenesis; subunit of U3-containing 90S preribosome processome complex involved in small ribosomal subunit assembly; stimulates Bms1p GTPase and U3 binding activity; similar to RNA cyclase-like proteins but no cyclase activity detected Gene:RPC53(YDL150W)|FD-Score:-3.18|P-value:7.39E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C53 Gene:RRB1(YMR131C)|FD-Score:3.55|P-value:1.95E-4|Clearance:0.03||SGD DESC:Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p Gene:SMD2(YLR275W)|FD-Score:4.88|P-value:5.42E-7|Clearance:0.04||SGD DESC:Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 Gene:THG1(YGR024C)|FD-Score:3.11|P-value:9.27E-4|Clearance:0.04||SGD DESC:tRNAHis guanylyltransferase, adds a guanosine residue to the 5' end of tRNAHis after transcription and RNase P cleavage; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes Gene:TPI1(YDR050C)|FD-Score:-3.42|P-value:3.18E-4|Clearance:0||SGD DESC:Triose phosphate isomerase, abundant glycolytic enzyme; mRNA half-life is regulated by iron availability; transcription is controlled by activators Reb1p, Gcr1p, and Rap1p through binding sites in the 5' non-coding region; inhibition of Tpi1p activity by PEP (phosphoenolpyruvate) stimulates redox metabolism in respiring cells; E104D mutation in human TPI causes a rare autosomal disease Gene:WBP1(YEL002C)|FD-Score:-3.75|P-value:8.96E-5|Clearance:0||SGD DESC:Beta subunit of the oligosaccharyl transferase (OST) glycoprotein complex; required for N-linked glycosylation of proteins in the endoplasmic reticulum Gene:YGR265W(YGR265W_d)|FD-Score:5.78|P-value:3.69E-9|Clearance:0.91||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase Gene:ACT1(YFL039C)|FD-Score:4.51|P-value:3.27E-6|Clearance:0.63||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:ALA1(YOR335C)|FD-Score:3.27|P-value:5.44E-4|Clearance:0.1||SGD DESC:Cytoplasmic and mitochondrial alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog Gene:BET2(YPR176C)|FD-Score:3.52|P-value:2.18E-4|Clearance:0.04||SGD DESC:Beta subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:BET4(YJL031C)|FD-Score:-3.12|P-value:8.97E-4|Clearance:0||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:BRN1(YBL097W)|FD-Score:3.82|P-value:6.79E-5|Clearance:0.27||SGD DESC:Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission Gene:CDC16(YKL022C)|FD-Score:3.13|P-value:8.75E-4|Clearance:0.02||SGD DESC:Subunit of the anaphase-promoting complex/cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation Gene:CDC48(YDL126C)|FD-Score:-3.12|P-value:9.08E-4|Clearance:0||SGD DESC:AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP Gene:CFD1(YIL003W)|FD-Score:-3.14|P-value:8.42E-4|Clearance:0||SGD DESC:Highly conserved, iron-sulfur cluster binding protein localized in the cytoplasm; forms a complex with Nbp35p that is involved in iron-sulfur protein assembly in the cytosol Gene:COP1(YDL145C)|FD-Score:3.48|P-value:2.54E-4|Clearance:0.01||SGD DESC:Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway Gene:DML1(YMR211W)|FD-Score:3.47|P-value:2.62E-4|Clearance:0.03||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:ERG8(YMR220W)|FD-Score:4.84|P-value:6.65E-7|Clearance:0.33||SGD DESC:Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:FAD1(YDL045C)|FD-Score:6.08|P-value:6.04E-10|Clearance:0.91||SGD DESC:Flavin adenine dinucleotide (FAD) synthetase, performs the second step in synthesis of FAD from riboflavin Gene:FAS1(YKL182W)|FD-Score:3.87|P-value:5.37E-5|Clearance:0||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:IPI1(YHR085W)|FD-Score:-4.66|P-value:1.61E-6|Clearance:0||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; IPI1 is an essential gene Gene:MED6(YHR058C)|FD-Score:-6.29|P-value:1.56E-10|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress Gene:MVD1(YNR043W)|FD-Score:-4.5|P-value:3.46E-6|Clearance:0||SGD DESC:Mevalonate pyrophosphate decarboxylase, essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer Gene:NSE3(YDR288W)|FD-Score:3.17|P-value:7.68E-4|Clearance:0.04||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress Gene:PHS1(YJL097W)|FD-Score:3.87|P-value:5.46E-5|Clearance:0.05||SGD DESC:Essential 3-hydroxyacyl-CoA dehydratase of the ER membrane, involved in elongation of very long-chain fatty acids; evolutionarily conserved, similar to mammalian PTPLA and PTPLB; involved in sphingolipid biosynthesis and protein trafficking Gene:RCL1(YOL010W)|FD-Score:3.44|P-value:2.89E-4|Clearance:0.17||SGD DESC:Endonuclease that cleaves pre-rRNA at site A2 for 18S rRNA biogenesis; subunit of U3-containing 90S preribosome processome complex involved in small ribosomal subunit assembly; stimulates Bms1p GTPase and U3 binding activity; similar to RNA cyclase-like proteins but no cyclase activity detected Gene:RPC53(YDL150W)|FD-Score:-3.18|P-value:7.39E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C53 Gene:RRB1(YMR131C)|FD-Score:3.55|P-value:1.95E-4|Clearance:0.03||SGD DESC:Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p Gene:SMD2(YLR275W)|FD-Score:4.88|P-value:5.42E-7|Clearance:0.04||SGD DESC:Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 Gene:THG1(YGR024C)|FD-Score:3.11|P-value:9.27E-4|Clearance:0.04||SGD DESC:tRNAHis guanylyltransferase, adds a guanosine residue to the 5' end of tRNAHis after transcription and RNase P cleavage; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes Gene:TPI1(YDR050C)|FD-Score:-3.42|P-value:3.18E-4|Clearance:0||SGD DESC:Triose phosphate isomerase, abundant glycolytic enzyme; mRNA half-life is regulated by iron availability; transcription is controlled by activators Reb1p, Gcr1p, and Rap1p through binding sites in the 5' non-coding region; inhibition of Tpi1p activity by PEP (phosphoenolpyruvate) stimulates redox metabolism in respiring cells; E104D mutation in human TPI causes a rare autosomal disease Gene:WBP1(YEL002C)|FD-Score:-3.75|P-value:8.96E-5|Clearance:0||SGD DESC:Beta subunit of the oligosaccharyl transferase (OST) glycoprotein complex; required for N-linked glycosylation of proteins in the endoplasmic reticulum Gene:YGR265W(YGR265W_d)|FD-Score:5.78|P-value:3.69E-9|Clearance:0.91||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6708711
Download HOP data (tab-delimited text)  (excel)
Gene:ALG6(YOR002W)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease Gene:APE3(YBR286W)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Vacuolar aminopeptidase Y, processed to mature form by Prb1p Gene:APT1(YML022W)|FD-Score:3.1|P-value:9.73E-4||SGD DESC:Adenine phosphoribosyltransferase; catalyzes the formation of AMP from adenine and 5-phosphoribosylpyrophosphate; involved in the salvage pathway of purine nucleotide biosynthesis; APT1 has a paralog, APT2, that arose from the whole genome duplication Gene:AST1(YBL069W)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:Lipid raft associated protein; interacts with the plasma membrane ATPase Pma1p and has a role in its targeting to the plasma membrane by influencing its incorporation into lipid rafts; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST1 has a paralog, AST2, that arose from the whole genome duplication Gene:ATF2(YGR177C)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Alcohol acetyltransferase, may play a role in steroid detoxification; forms volatile esters during fermentation, which is important for brewing and winemaking Gene:ATP23(YNR020C)|FD-Score:3.12|P-value:8.98E-4||SGD DESC:Putative metalloprotease of the mitochondrial inner membrane, required for processing of Atp6p; has an additional role in assembly of the F0 sector of the F1F0 ATP synthase complex; substrate of the Mia40p-Erv1p disulfide relay system, and folding is assisted by Mia40p Gene:ATP5(YDR298C)|FD-Score:-4.04|P-value:2.70E-5||SGD DESC:Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated Gene:BMH2(YDR099W)|FD-Score:3.11|P-value:9.44E-4||SGD DESC:14-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:CCC1(YLR220W)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Putative vacuolar Fe2+/Mn2+ transporter; suppresses respiratory deficit of yfh1 mutants, which lack the ortholog of mammalian frataxin, by preventing mitochondrial iron accumulation; relative distribution to the vacuole decreases upon DNA replication stress Gene:CCR4(YAL021C)|FD-Score:4.42|P-value:4.90E-6||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:CIN8(YEL061C)|FD-Score:-4.1|P-value:2.09E-5||SGD DESC:Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation Gene:CNN1(YFR046C)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:Kinetochore protein; associated with the essential kinetochore proteins Nnf1p and Spc24p; phosphorylated by Clb5-Cdk1, Mps1p, Ipl1p and to a lesser extent by Clb2-Cdk1; localizes to the lower region of the Ndc80 complex during anaphase and regulates KMN activity by inhibiting the Mtw1 and Spc105 complexes from binding to the Ndc80 complex; similar to metazoan CENP-T Gene:CPR3(YML078W)|FD-Score:-3.17|P-value:7.60E-4||SGD DESC:Mitochondrial peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; involved in protein refolding after import into mitochondria Gene:DCS1(YLR270W)|FD-Score:-3.56|P-value:1.86E-4||SGD DESC:Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress Gene:DEF1(YKL054C)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and proteolysis of RNAPII present in an elongation complex; mutant is deficient in Zip1p loading onto chromosomes during meiosis Gene:DID4(YKL002W)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis Gene:DLD2(YDL178W)|FD-Score:-3.38|P-value:3.57E-4||SGD DESC:D-lactate dehydrogenase, located in the mitochondrial matrix Gene:DPL1(YDR294C)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:Dihydrosphingosine phosphate lyase, regulates intracellular levels of sphingolipid long-chain base phosphates (LCBPs), degrades phosphorylated long chain bases, prefers C16 dihydrosphingosine-l-phosphate as a substrate Gene:ECM29(YHL030W)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Scaffold protein; assists in association of the proteasome core particle with the regulatory particle; degraded by the mature proteasome after assembly; contains HEAT-like repeats; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:ERG4(YGL012W)|FD-Score:4.07|P-value:2.33E-5||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FCY1(YPR062W)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU) Gene:FMP48(YGR052W_p)|FD-Score:-3.58|P-value:1.71E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FRE2(YKL220C)|FD-Score:3.11|P-value:9.21E-4||SGD DESC:Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low iron levels but not by low copper levels Gene:GCV2(YMR189W)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:P subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of 5,10-methylene-THF in the cytoplasm Gene:GTR2(YGR163W)|FD-Score:5.04|P-value:2.35E-7||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:HAM1(YJR069C)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Nucleoside triphosphate pyrophosphohydrolase; active against a wide range of substrates including ITP, dITP and XTP; mediates exclusion of noncanonical purines and pyrimadines from deoxyribonucleoside triphosphate pools; mutant is sensitive to the base analog 6-N-hydroxylaminopurine; protein abundance increases in response to DNA replication stress Gene:HAT2(YEL056W)|FD-Score:3.43|P-value:3.01E-4||SGD DESC:Subunit of the Hat1p-Hat2p histone acetyltransferase complex; required for high affinity binding of the complex to free histone H4, thereby enhancing Hat1p activity; similar to human RbAp46 and 48; has a role in telomeric silencing Gene:HFA1(YMR207C)|FD-Score:3.26|P-value:5.65E-4||SGD DESC:Mitochondrial acetyl-coenzyme A carboxylase; catalyzes the production of malonyl-CoA in mitochondrial fatty acid biosynthesis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:HOS4(YIL112W)|FD-Score:-4.57|P-value:2.49E-6||SGD DESC:Subunit of the Set3 complex, which is a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; potential Cdc28p substrate Gene:IFM1(YOL023W)|FD-Score:-3.27|P-value:5.31E-4||SGD DESC:Mitochondrial translation initiation factor 2 Gene:IGD1(YFR017C)|FD-Score:3.4|P-value:3.41E-4||SGD DESC:Cytoplasmic protein that inhibits Gdb1p glycogen debranching activity; required for normal intracellular accumulation of glycogen; phosphorylated in vivo; expression increases during wine fermentation; protein abundance increases in response to DNA replication stress; IGD1 has a paralog, YOL024W, that arose from the whole genome duplication Gene:IRS4(YKR019C)|FD-Score:4.61|P-value:1.97E-6||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:IWR1(YDL115C)|FD-Score:-4.66|P-value:1.56E-6||SGD DESC:RNA polymerase II transport factor, conserved from yeast to humans; also has a role in transporting RNA polymerase III into the nucleus; interacts with most of the RNAP II subunits; nucleo-cytoplasmic shuttling protein; deletion causes hypersensitivity to K1 killer toxin; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:MDR1(YGR100W)|FD-Score:3.82|P-value:6.75E-5||SGD DESC:Cytoplasmic GTPase-activating protein for Ypt/Rab transport GTPases Ypt6p, Ypt31p and Sec4p; involved in recycling of internalized proteins and regulation of Golgi secretory function Gene:MNR2(YKL064W)|FD-Score:3.2|P-value:6.79E-4||SGD DESC:Vacuolar membrane protein required for magnesium homeostasis; putative magnesium transporter; has similarity to Alr1p and Alr2p, which mediate influx of Mg2+ and other divalent cations Gene:MRC1(YCL061C)|FD-Score:-4.04|P-value:2.69E-5||SGD DESC:S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres Gene:MRPS28(YDR337W)|FD-Score:-3.57|P-value:1.79E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSG5(YNL053W)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Dual-specificity protein phosphatase; exists in 2 isoforms; required for maintenance of a low level of signaling through the cell integrity pathway, adaptive response to pheromone; regulates and is regulated by Slt2p; dephosphorylates Fus3p Gene:MSS116(YDR194C)|FD-Score:-3.22|P-value:6.50E-4||SGD DESC:DEAD-box protein required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing Gene:NPL4(YBR170C)|FD-Score:-3.79|P-value:7.39E-5||SGD DESC:Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; assists Cdc48p in the dislocation of misfolded, polyubiquitinated ERAD substrates that are subsequently delivered to the proteasome for degradation; also involved in the regulated destruction of resident ER membrane proteins, such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing (RUP) Gene:PET18(YCR020C)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Protein of unknown function, has weak similarity to proteins involved in thiamin metabolism; expression is induced in the absence of thiamin Gene:PMT2(YAL023C)|FD-Score:-3.38|P-value:3.65E-4||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; involved in ER quality control; acts in a complex with Pmt1p, can instead interact with Pmt5p; antifungal drug target; PMT2 has a paralog, PMT3, that arose from the whole genome duplication Gene:PMT6(YGR199W)|FD-Score:3.3|P-value:4.79E-4||SGD DESC:Protein O-mannosyltransferase, transfers mannose from dolichyl phosphate-D-mannose to protein serine/threonine residues of secretory proteins; reaction is essential for cell wall rigidity; member of a family of mannosyltransferases Gene:PRB1(YEL060C)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Vacuolar proteinase B (yscB); serine protease of the subtilisin family; involved in protein degradation in the vacuole and required for full protein degradation during sporulation; activity inhibited by Pbi2p; protein abundance increases in response to DNA replication stress Gene:PRM3(YPL192C)|FD-Score:3.74|P-value:9.07E-5||SGD DESC:Pheromone-regulated protein required for nuclear envelope fusion during karyogamy; localizes to the outer face of the nuclear membrane; interacts with Kar5p at the spindle pole body Gene:PSH1(YOL054W)|FD-Score:4.38|P-value:5.95E-6||SGD DESC:E3 ubiquitin ligase that mediates poyubiquitination and degradation of centromere-binding protein Cse4p and prevents Cse4p from mislocalizing to euchromatin; ubiquitylation of Cse4p may be antagonized by Scm3p Gene:PUS2(YGL063W)|FD-Score:-4.45|P-value:4.27E-6||SGD DESC:Mitochondrial tRNA:pseudouridine synthase; acts at positions 27 and 28, but not at position 72; efficiently and rapidly targeted to mitochondria, specifically dedicated to mitochondrial tRNA modification Gene:PXL1(YKR090W)|FD-Score:3.75|P-value:8.68E-5||SGD DESC:Protein that localizes to sites of polarized growth; required for selection and/or maintenance of polarized growth sites, may modulate signaling by the GTPases Cdc42p and Rho1p; contains LIM domains and has similarity to metazoan paxillin; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RAD33(YML011C)|FD-Score:4.94|P-value:3.87E-7||SGD DESC:Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:RAX1(YOR301W)|FD-Score:3.75|P-value:8.79E-5||SGD DESC:Protein involved in bud site selection during bipolar budding; localization requires Rax2p; has similarity to members of the insulin-related peptide superfamily Gene:RHR2(YIL053W)|FD-Score:-3.18|P-value:7.41E-4||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:RMA1(YKL132C)|FD-Score:3.17|P-value:7.55E-4||SGD DESC:Putative dihydrofolate synthetase; has similarity to Fol3p and to E. coli folylpolyglutamate synthetase/dihydrofolate synthetase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RNH202(YDR279W)|FD-Score:3.17|P-value:7.52E-4||SGD DESC:Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS2 that causes Aicardi-Goutieres syndrome Gene:RPE1(YJL121C)|FD-Score:-3.25|P-value:5.78E-4||SGD DESC:D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress Gene:RPL37B(YDR500C)|FD-Score:-3.75|P-value:8.94E-5||SGD DESC:Ribosomal 60S subunit protein L37B; protein abundance increases in response to DNA replication stress; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37B has a paralog, RPL37A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:-3.47|P-value:2.59E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS4A(YJR145C)|FD-Score:-3.1|P-value:9.52E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4A has a paralog, RPS4B, that arose from the whole genome duplication Gene:RRT14(YIL127C_p)|FD-Score:3.75|P-value:8.80E-5||SGD DESC:Putative protein of unknown function; identified in a screen for mutants with decreased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis Gene:RRT1(YBL048W_d)|FD-Score:4.3|P-value:8.53E-6||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:RTC3(YHR087W)|FD-Score:-3.84|P-value:6.07E-5||SGD DESC:Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity; protein abundance increases in response to DNA replication stress Gene:SCP1(YOR367W)|FD-Score:3.49|P-value:2.37E-4||SGD DESC:Component of yeast cortical actin cytoskeleton, binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin Gene:SDC1(YDR469W)|FD-Score:3.22|P-value:6.31E-4||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30 Gene:SDH2(YLL041C)|FD-Score:3.88|P-value:5.33E-5||SGD DESC:Iron-sulfur protein subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain Gene:SEC28(YIL076W)|FD-Score:-3.62|P-value:1.47E-4||SGD DESC:Epsilon-COP subunit of the coatomer; regulates retrograde Golgi-to-ER protein traffic; stabilizes Cop1p, the alpha-COP and the coatomer complex; non-essential for cell growth; protein abundance increases in response to DNA replication stress Gene:SEH1(YGL100W)|FD-Score:-4.28|P-value:9.15E-6||SGD DESC:Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 Gene:SHE2(YKL130C)|FD-Score:5.5|P-value:1.95E-8||SGD DESC:RNA-binding protein that binds specific mRNAs and interacts with She3p; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud Gene:SIP4(YJL089W)|FD-Score:-3.18|P-value:7.25E-4||SGD DESC:C6 zinc cluster transcriptional activator that binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus Gene:SKG3(YLR187W_p)|FD-Score:4.58|P-value:2.32E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Skg4p; relocalizes from bud neck to cytoplasm upon DNA replication stress; SKG3 has a paralog, CAF120, that arose from the whole genome duplication Gene:SNO1(YMR095C)|FD-Score:3.34|P-value:4.20E-4||SGD DESC:Protein of unconfirmed function, involved in pyridoxine metabolism; expression is induced during stationary phase; forms a putative glutamine amidotransferase complex with Snz1p, with Sno1p serving as the glutaminase Gene:SPT23(YKL020C)|FD-Score:4.1|P-value:2.02E-5||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Mga2p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:SRB8(YCR081W)|FD-Score:-5.09|P-value:1.79E-7||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:SSD1(YDR293C)|FD-Score:-3.5|P-value:2.29E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SSE1(YPL106C)|FD-Score:-3.62|P-value:1.44E-4||SGD DESC:ATPase component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSE1 has a paralog, SSE2, that arose from the whole genome duplication Gene:STB4(YMR019W)|FD-Score:3.2|P-value:6.90E-4||SGD DESC:Putative transcription factor; contains a Zn(II)2Cys6 zinc finger domain characteristic of DNA-binding proteins; computational analysis suggests a role in regulation of expression of genes encoding transporters; binds Sin3p in a two-hybrid assay; Gene:STR2(YJR130C)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Cystathionine gamma-synthase, converts cysteine into cystathionine Gene:TIS11(YLR136C)|FD-Score:3.87|P-value:5.38E-5||SGD DESC:mRNA-binding protein expressed during iron starvation; binds to a sequence element in the 3'-untranslated regions of specific mRNAs to mediate their degradation; involved in iron homeostasis; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:TMA16(YOR252W)|FD-Score:4.4|P-value:5.49E-6||SGD DESC:Protein of unknown function that associates with ribosomes Gene:TRM13(YOL125W)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:2'-O-methyltransferase responsible for modification of tRNA at position 4; C-terminal domain has similarity to Rossmann-fold (RFM) superfamily of RNA methyltransferases Gene:UIP3(YAR027W)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Putative integral membrane protein of unknown function; interacts with Ulp1p at the nuclear periphery; member of DUP240 gene family Gene:URA8(YJR103W)|FD-Score:4.51|P-value:3.32E-6||SGD DESC:Minor CTP synthase isozyme (see also URA7); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA8 has a paralog, URA7, that arose from the whole genome duplication Gene:URE2(YNL229C)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VNX1(YNL321W)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Calcium/H+ antiporter localized to the endoplasmic reticulum membrane; member of the calcium exchanger (CAX) family; potential Cdc28p substrate Gene:VPS72(YDR485C)|FD-Score:-3.88|P-value:5.29E-5||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:VTH1(YIL173W)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Putative membrane glycoprotein with strong similarity to Vth2p and Pep1p/Vps10p, may be involved in vacuolar protein sorting Gene:YBR071W(YBR071W)|FD-Score:4.02|P-value:2.85E-5||SGD DESC:Protein of unknown function found in the cytoplasm and bud neck; mRNA expression may be regulated by the cell cycle and/or cell wall stress; overexpression of YBR071W affects endocytic protein trafficking Gene:YBR238C(YBR238C)|FD-Score:-3.46|P-value:2.66E-4||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YEL1(YBL060W)|FD-Score:7.21|P-value:2.87E-13||SGD DESC:Guanine nucleotide exchange factor specific for Arf3p; localized to the bud neck and tip; required for localization of Arf3p to the bud neck and tip Gene:YER046W-A(YER046W-A_d)|FD-Score:3.85|P-value:5.92E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER077C(YER077C_p)|FD-Score:-3.65|P-value:1.33E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport Gene:YER130C(YER130C_p)|FD-Score:-5.88|P-value:2.07E-9||SGD DESC:Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress Gene:YER156C(YER156C_p)|FD-Score:-3.51|P-value:2.26E-4||SGD DESC:Putative protein of unknown function; interacts with Hsp82p and copurifies with Ipl1p; expression is copper responsive and downregulated in strains deleted for MAC1, a copper-responsive transcription factor; similarity to mammalian MYG1 Gene:YGR035C(YGR035C_p)|FD-Score:-3.76|P-value:8.65E-5||SGD DESC:Putative protein of unknown function, potential Cdc28p substrate; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance Gene:YGR053C(YGR053C_p)|FD-Score:4.58|P-value:2.29E-6||SGD DESC:Putative protein of unknown function Gene:YHL026C(YHL026C_p)|FD-Score:4|P-value:3.16E-5||SGD DESC:Putative protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; YHL026C is not an essential gene; in 2005 the start site was moved 141 nt upstream (see Locus History) Gene:YJR107W(YJR107W_p)|FD-Score:3.27|P-value:5.44E-4||SGD DESC:Putative lipase Gene:YKL131W(YKL131W_d)|FD-Score:4.17|P-value:1.51E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL222C(YKL222C)|FD-Score:7.34|P-value:1.10E-13||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; similar to transcriptional regulators from the zinc cluster (binuclear cluster) family; null mutant is sensitive to caffeine Gene:YLR031W(YLR031W_p)|FD-Score:-3.22|P-value:6.38E-4||SGD DESC:Putative protein of unknown function Gene:YLR294C(YLR294C_d)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ATP14 Gene:YLR307C-A(YLR307C-A_p)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Putative protein of unknown function Gene:YLR349W(YLR349W_d)|FD-Score:3.09|P-value:9.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified ORF DIC1/YLR348C Gene:YLR416C(YLR416C_d)|FD-Score:-3.22|P-value:6.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMD8(YML038C)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Putative nucleotide sugar transporter, has similarity to Vrg4p Gene:YML018C(YML018C_p)|FD-Score:-6.9|P-value:2.68E-12||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication Gene:YMR147W(YMR147W_p)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Putative protein of unknown function Gene:YMR210W(YMR210W)|FD-Score:5.44|P-value:2.60E-8||SGD DESC:Putative acyltransferase with similarity to Eeb1p and Eht1p, has a minor role in medium-chain fatty acid ethyl ester biosynthesis; may be involved in lipid metabolism and detoxification Gene:YND1(YER005W)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Apyrase with wide substrate specificity, helps prevent inhibition of glycosylation by hydrolyzing nucleoside tri- and diphosphates that inhibit glycotransferases; partially redundant with Gda1p; mediates adenovirus E4orf4-induced toxicity Gene:YNL338W(YNL338W_d)|FD-Score:4.72|P-value:1.19E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps TEL14L-XC, which is Telomeric X element Core sequence on the left arm of Chromosome XIV Gene:YOL162W(YOL162W_p)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family Gene:YOR111W(YOR111W_p)|FD-Score:3.9|P-value:4.81E-5||SGD DESC:Putative protein of unknown function Gene:YPL039W(YPL039W_p)|FD-Score:-3.58|P-value:1.69E-4||SGD DESC:Putative protein of unknown function; YPL039W is not an essential gene Gene:YPL107W(YPL107W_p)|FD-Score:5.7|P-value:5.97E-9||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YPL107W is not an essential gene Gene:YPL108W(YPL108W_p)|FD-Score:-3.51|P-value:2.22E-4||SGD DESC:Cytoplasmic protein of unknown function; non-essential gene that is induced in a GDH1 deleted strain with altered redox metabolism; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YPT35(YHR105W)|FD-Score:-3.11|P-value:9.26E-4||SGD DESC:Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport Gene:ALG6(YOR002W)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease Gene:APE3(YBR286W)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Vacuolar aminopeptidase Y, processed to mature form by Prb1p Gene:APT1(YML022W)|FD-Score:3.1|P-value:9.73E-4||SGD DESC:Adenine phosphoribosyltransferase; catalyzes the formation of AMP from adenine and 5-phosphoribosylpyrophosphate; involved in the salvage pathway of purine nucleotide biosynthesis; APT1 has a paralog, APT2, that arose from the whole genome duplication Gene:AST1(YBL069W)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:Lipid raft associated protein; interacts with the plasma membrane ATPase Pma1p and has a role in its targeting to the plasma membrane by influencing its incorporation into lipid rafts; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST1 has a paralog, AST2, that arose from the whole genome duplication Gene:ATF2(YGR177C)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Alcohol acetyltransferase, may play a role in steroid detoxification; forms volatile esters during fermentation, which is important for brewing and winemaking Gene:ATP23(YNR020C)|FD-Score:3.12|P-value:8.98E-4||SGD DESC:Putative metalloprotease of the mitochondrial inner membrane, required for processing of Atp6p; has an additional role in assembly of the F0 sector of the F1F0 ATP synthase complex; substrate of the Mia40p-Erv1p disulfide relay system, and folding is assisted by Mia40p Gene:ATP5(YDR298C)|FD-Score:-4.04|P-value:2.70E-5||SGD DESC:Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated Gene:BMH2(YDR099W)|FD-Score:3.11|P-value:9.44E-4||SGD DESC:14-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:CCC1(YLR220W)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Putative vacuolar Fe2+/Mn2+ transporter; suppresses respiratory deficit of yfh1 mutants, which lack the ortholog of mammalian frataxin, by preventing mitochondrial iron accumulation; relative distribution to the vacuole decreases upon DNA replication stress Gene:CCR4(YAL021C)|FD-Score:4.42|P-value:4.90E-6||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:CIN8(YEL061C)|FD-Score:-4.1|P-value:2.09E-5||SGD DESC:Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation Gene:CNN1(YFR046C)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:Kinetochore protein; associated with the essential kinetochore proteins Nnf1p and Spc24p; phosphorylated by Clb5-Cdk1, Mps1p, Ipl1p and to a lesser extent by Clb2-Cdk1; localizes to the lower region of the Ndc80 complex during anaphase and regulates KMN activity by inhibiting the Mtw1 and Spc105 complexes from binding to the Ndc80 complex; similar to metazoan CENP-T Gene:CPR3(YML078W)|FD-Score:-3.17|P-value:7.60E-4||SGD DESC:Mitochondrial peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; involved in protein refolding after import into mitochondria Gene:DCS1(YLR270W)|FD-Score:-3.56|P-value:1.86E-4||SGD DESC:Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress Gene:DEF1(YKL054C)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and proteolysis of RNAPII present in an elongation complex; mutant is deficient in Zip1p loading onto chromosomes during meiosis Gene:DID4(YKL002W)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis Gene:DLD2(YDL178W)|FD-Score:-3.38|P-value:3.57E-4||SGD DESC:D-lactate dehydrogenase, located in the mitochondrial matrix Gene:DPL1(YDR294C)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:Dihydrosphingosine phosphate lyase, regulates intracellular levels of sphingolipid long-chain base phosphates (LCBPs), degrades phosphorylated long chain bases, prefers C16 dihydrosphingosine-l-phosphate as a substrate Gene:ECM29(YHL030W)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Scaffold protein; assists in association of the proteasome core particle with the regulatory particle; degraded by the mature proteasome after assembly; contains HEAT-like repeats; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:ERG4(YGL012W)|FD-Score:4.07|P-value:2.33E-5||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FCY1(YPR062W)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU) Gene:FMP48(YGR052W_p)|FD-Score:-3.58|P-value:1.71E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FRE2(YKL220C)|FD-Score:3.11|P-value:9.21E-4||SGD DESC:Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low iron levels but not by low copper levels Gene:GCV2(YMR189W)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:P subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of 5,10-methylene-THF in the cytoplasm Gene:GTR2(YGR163W)|FD-Score:5.04|P-value:2.35E-7||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:HAM1(YJR069C)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Nucleoside triphosphate pyrophosphohydrolase; active against a wide range of substrates including ITP, dITP and XTP; mediates exclusion of noncanonical purines and pyrimadines from deoxyribonucleoside triphosphate pools; mutant is sensitive to the base analog 6-N-hydroxylaminopurine; protein abundance increases in response to DNA replication stress Gene:HAT2(YEL056W)|FD-Score:3.43|P-value:3.01E-4||SGD DESC:Subunit of the Hat1p-Hat2p histone acetyltransferase complex; required for high affinity binding of the complex to free histone H4, thereby enhancing Hat1p activity; similar to human RbAp46 and 48; has a role in telomeric silencing Gene:HFA1(YMR207C)|FD-Score:3.26|P-value:5.65E-4||SGD DESC:Mitochondrial acetyl-coenzyme A carboxylase; catalyzes the production of malonyl-CoA in mitochondrial fatty acid biosynthesis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:HOS4(YIL112W)|FD-Score:-4.57|P-value:2.49E-6||SGD DESC:Subunit of the Set3 complex, which is a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; potential Cdc28p substrate Gene:IFM1(YOL023W)|FD-Score:-3.27|P-value:5.31E-4||SGD DESC:Mitochondrial translation initiation factor 2 Gene:IGD1(YFR017C)|FD-Score:3.4|P-value:3.41E-4||SGD DESC:Cytoplasmic protein that inhibits Gdb1p glycogen debranching activity; required for normal intracellular accumulation of glycogen; phosphorylated in vivo; expression increases during wine fermentation; protein abundance increases in response to DNA replication stress; IGD1 has a paralog, YOL024W, that arose from the whole genome duplication Gene:IRS4(YKR019C)|FD-Score:4.61|P-value:1.97E-6||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:IWR1(YDL115C)|FD-Score:-4.66|P-value:1.56E-6||SGD DESC:RNA polymerase II transport factor, conserved from yeast to humans; also has a role in transporting RNA polymerase III into the nucleus; interacts with most of the RNAP II subunits; nucleo-cytoplasmic shuttling protein; deletion causes hypersensitivity to K1 killer toxin; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:MDR1(YGR100W)|FD-Score:3.82|P-value:6.75E-5||SGD DESC:Cytoplasmic GTPase-activating protein for Ypt/Rab transport GTPases Ypt6p, Ypt31p and Sec4p; involved in recycling of internalized proteins and regulation of Golgi secretory function Gene:MNR2(YKL064W)|FD-Score:3.2|P-value:6.79E-4||SGD DESC:Vacuolar membrane protein required for magnesium homeostasis; putative magnesium transporter; has similarity to Alr1p and Alr2p, which mediate influx of Mg2+ and other divalent cations Gene:MRC1(YCL061C)|FD-Score:-4.04|P-value:2.69E-5||SGD DESC:S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres Gene:MRPS28(YDR337W)|FD-Score:-3.57|P-value:1.79E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSG5(YNL053W)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Dual-specificity protein phosphatase; exists in 2 isoforms; required for maintenance of a low level of signaling through the cell integrity pathway, adaptive response to pheromone; regulates and is regulated by Slt2p; dephosphorylates Fus3p Gene:MSS116(YDR194C)|FD-Score:-3.22|P-value:6.50E-4||SGD DESC:DEAD-box protein required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing Gene:NPL4(YBR170C)|FD-Score:-3.79|P-value:7.39E-5||SGD DESC:Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; assists Cdc48p in the dislocation of misfolded, polyubiquitinated ERAD substrates that are subsequently delivered to the proteasome for degradation; also involved in the regulated destruction of resident ER membrane proteins, such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing (RUP) Gene:PET18(YCR020C)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Protein of unknown function, has weak similarity to proteins involved in thiamin metabolism; expression is induced in the absence of thiamin Gene:PMT2(YAL023C)|FD-Score:-3.38|P-value:3.65E-4||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; involved in ER quality control; acts in a complex with Pmt1p, can instead interact with Pmt5p; antifungal drug target; PMT2 has a paralog, PMT3, that arose from the whole genome duplication Gene:PMT6(YGR199W)|FD-Score:3.3|P-value:4.79E-4||SGD DESC:Protein O-mannosyltransferase, transfers mannose from dolichyl phosphate-D-mannose to protein serine/threonine residues of secretory proteins; reaction is essential for cell wall rigidity; member of a family of mannosyltransferases Gene:PRB1(YEL060C)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Vacuolar proteinase B (yscB); serine protease of the subtilisin family; involved in protein degradation in the vacuole and required for full protein degradation during sporulation; activity inhibited by Pbi2p; protein abundance increases in response to DNA replication stress Gene:PRM3(YPL192C)|FD-Score:3.74|P-value:9.07E-5||SGD DESC:Pheromone-regulated protein required for nuclear envelope fusion during karyogamy; localizes to the outer face of the nuclear membrane; interacts with Kar5p at the spindle pole body Gene:PSH1(YOL054W)|FD-Score:4.38|P-value:5.95E-6||SGD DESC:E3 ubiquitin ligase that mediates poyubiquitination and degradation of centromere-binding protein Cse4p and prevents Cse4p from mislocalizing to euchromatin; ubiquitylation of Cse4p may be antagonized by Scm3p Gene:PUS2(YGL063W)|FD-Score:-4.45|P-value:4.27E-6||SGD DESC:Mitochondrial tRNA:pseudouridine synthase; acts at positions 27 and 28, but not at position 72; efficiently and rapidly targeted to mitochondria, specifically dedicated to mitochondrial tRNA modification Gene:PXL1(YKR090W)|FD-Score:3.75|P-value:8.68E-5||SGD DESC:Protein that localizes to sites of polarized growth; required for selection and/or maintenance of polarized growth sites, may modulate signaling by the GTPases Cdc42p and Rho1p; contains LIM domains and has similarity to metazoan paxillin; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RAD33(YML011C)|FD-Score:4.94|P-value:3.87E-7||SGD DESC:Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:RAX1(YOR301W)|FD-Score:3.75|P-value:8.79E-5||SGD DESC:Protein involved in bud site selection during bipolar budding; localization requires Rax2p; has similarity to members of the insulin-related peptide superfamily Gene:RHR2(YIL053W)|FD-Score:-3.18|P-value:7.41E-4||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:RMA1(YKL132C)|FD-Score:3.17|P-value:7.55E-4||SGD DESC:Putative dihydrofolate synthetase; has similarity to Fol3p and to E. coli folylpolyglutamate synthetase/dihydrofolate synthetase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RNH202(YDR279W)|FD-Score:3.17|P-value:7.52E-4||SGD DESC:Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS2 that causes Aicardi-Goutieres syndrome Gene:RPE1(YJL121C)|FD-Score:-3.25|P-value:5.78E-4||SGD DESC:D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress Gene:RPL37B(YDR500C)|FD-Score:-3.75|P-value:8.94E-5||SGD DESC:Ribosomal 60S subunit protein L37B; protein abundance increases in response to DNA replication stress; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37B has a paralog, RPL37A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:-3.47|P-value:2.59E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS4A(YJR145C)|FD-Score:-3.1|P-value:9.52E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4A has a paralog, RPS4B, that arose from the whole genome duplication Gene:RRT14(YIL127C_p)|FD-Score:3.75|P-value:8.80E-5||SGD DESC:Putative protein of unknown function; identified in a screen for mutants with decreased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis Gene:RRT1(YBL048W_d)|FD-Score:4.3|P-value:8.53E-6||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:RTC3(YHR087W)|FD-Score:-3.84|P-value:6.07E-5||SGD DESC:Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity; protein abundance increases in response to DNA replication stress Gene:SCP1(YOR367W)|FD-Score:3.49|P-value:2.37E-4||SGD DESC:Component of yeast cortical actin cytoskeleton, binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin Gene:SDC1(YDR469W)|FD-Score:3.22|P-value:6.31E-4||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30 Gene:SDH2(YLL041C)|FD-Score:3.88|P-value:5.33E-5||SGD DESC:Iron-sulfur protein subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain Gene:SEC28(YIL076W)|FD-Score:-3.62|P-value:1.47E-4||SGD DESC:Epsilon-COP subunit of the coatomer; regulates retrograde Golgi-to-ER protein traffic; stabilizes Cop1p, the alpha-COP and the coatomer complex; non-essential for cell growth; protein abundance increases in response to DNA replication stress Gene:SEH1(YGL100W)|FD-Score:-4.28|P-value:9.15E-6||SGD DESC:Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 Gene:SHE2(YKL130C)|FD-Score:5.5|P-value:1.95E-8||SGD DESC:RNA-binding protein that binds specific mRNAs and interacts with She3p; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud Gene:SIP4(YJL089W)|FD-Score:-3.18|P-value:7.25E-4||SGD DESC:C6 zinc cluster transcriptional activator that binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus Gene:SKG3(YLR187W_p)|FD-Score:4.58|P-value:2.32E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Skg4p; relocalizes from bud neck to cytoplasm upon DNA replication stress; SKG3 has a paralog, CAF120, that arose from the whole genome duplication Gene:SNO1(YMR095C)|FD-Score:3.34|P-value:4.20E-4||SGD DESC:Protein of unconfirmed function, involved in pyridoxine metabolism; expression is induced during stationary phase; forms a putative glutamine amidotransferase complex with Snz1p, with Sno1p serving as the glutaminase Gene:SPT23(YKL020C)|FD-Score:4.1|P-value:2.02E-5||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Mga2p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:SRB8(YCR081W)|FD-Score:-5.09|P-value:1.79E-7||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:SSD1(YDR293C)|FD-Score:-3.5|P-value:2.29E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SSE1(YPL106C)|FD-Score:-3.62|P-value:1.44E-4||SGD DESC:ATPase component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSE1 has a paralog, SSE2, that arose from the whole genome duplication Gene:STB4(YMR019W)|FD-Score:3.2|P-value:6.90E-4||SGD DESC:Putative transcription factor; contains a Zn(II)2Cys6 zinc finger domain characteristic of DNA-binding proteins; computational analysis suggests a role in regulation of expression of genes encoding transporters; binds Sin3p in a two-hybrid assay; Gene:STR2(YJR130C)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Cystathionine gamma-synthase, converts cysteine into cystathionine Gene:TIS11(YLR136C)|FD-Score:3.87|P-value:5.38E-5||SGD DESC:mRNA-binding protein expressed during iron starvation; binds to a sequence element in the 3'-untranslated regions of specific mRNAs to mediate their degradation; involved in iron homeostasis; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:TMA16(YOR252W)|FD-Score:4.4|P-value:5.49E-6||SGD DESC:Protein of unknown function that associates with ribosomes Gene:TRM13(YOL125W)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:2'-O-methyltransferase responsible for modification of tRNA at position 4; C-terminal domain has similarity to Rossmann-fold (RFM) superfamily of RNA methyltransferases Gene:UIP3(YAR027W)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Putative integral membrane protein of unknown function; interacts with Ulp1p at the nuclear periphery; member of DUP240 gene family Gene:URA8(YJR103W)|FD-Score:4.51|P-value:3.32E-6||SGD DESC:Minor CTP synthase isozyme (see also URA7); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA8 has a paralog, URA7, that arose from the whole genome duplication Gene:URE2(YNL229C)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VNX1(YNL321W)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Calcium/H+ antiporter localized to the endoplasmic reticulum membrane; member of the calcium exchanger (CAX) family; potential Cdc28p substrate Gene:VPS72(YDR485C)|FD-Score:-3.88|P-value:5.29E-5||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:VTH1(YIL173W)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Putative membrane glycoprotein with strong similarity to Vth2p and Pep1p/Vps10p, may be involved in vacuolar protein sorting Gene:YBR071W(YBR071W)|FD-Score:4.02|P-value:2.85E-5||SGD DESC:Protein of unknown function found in the cytoplasm and bud neck; mRNA expression may be regulated by the cell cycle and/or cell wall stress; overexpression of YBR071W affects endocytic protein trafficking Gene:YBR238C(YBR238C)|FD-Score:-3.46|P-value:2.66E-4||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YEL1(YBL060W)|FD-Score:7.21|P-value:2.87E-13||SGD DESC:Guanine nucleotide exchange factor specific for Arf3p; localized to the bud neck and tip; required for localization of Arf3p to the bud neck and tip Gene:YER046W-A(YER046W-A_d)|FD-Score:3.85|P-value:5.92E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER077C(YER077C_p)|FD-Score:-3.65|P-value:1.33E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport Gene:YER130C(YER130C_p)|FD-Score:-5.88|P-value:2.07E-9||SGD DESC:Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress Gene:YER156C(YER156C_p)|FD-Score:-3.51|P-value:2.26E-4||SGD DESC:Putative protein of unknown function; interacts with Hsp82p and copurifies with Ipl1p; expression is copper responsive and downregulated in strains deleted for MAC1, a copper-responsive transcription factor; similarity to mammalian MYG1 Gene:YGR035C(YGR035C_p)|FD-Score:-3.76|P-value:8.65E-5||SGD DESC:Putative protein of unknown function, potential Cdc28p substrate; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance Gene:YGR053C(YGR053C_p)|FD-Score:4.58|P-value:2.29E-6||SGD DESC:Putative protein of unknown function Gene:YHL026C(YHL026C_p)|FD-Score:4|P-value:3.16E-5||SGD DESC:Putative protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; YHL026C is not an essential gene; in 2005 the start site was moved 141 nt upstream (see Locus History) Gene:YJR107W(YJR107W_p)|FD-Score:3.27|P-value:5.44E-4||SGD DESC:Putative lipase Gene:YKL131W(YKL131W_d)|FD-Score:4.17|P-value:1.51E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL222C(YKL222C)|FD-Score:7.34|P-value:1.10E-13||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; similar to transcriptional regulators from the zinc cluster (binuclear cluster) family; null mutant is sensitive to caffeine Gene:YLR031W(YLR031W_p)|FD-Score:-3.22|P-value:6.38E-4||SGD DESC:Putative protein of unknown function Gene:YLR294C(YLR294C_d)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ATP14 Gene:YLR307C-A(YLR307C-A_p)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Putative protein of unknown function Gene:YLR349W(YLR349W_d)|FD-Score:3.09|P-value:9.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified ORF DIC1/YLR348C Gene:YLR416C(YLR416C_d)|FD-Score:-3.22|P-value:6.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMD8(YML038C)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Putative nucleotide sugar transporter, has similarity to Vrg4p Gene:YML018C(YML018C_p)|FD-Score:-6.9|P-value:2.68E-12||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication Gene:YMR147W(YMR147W_p)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Putative protein of unknown function Gene:YMR210W(YMR210W)|FD-Score:5.44|P-value:2.60E-8||SGD DESC:Putative acyltransferase with similarity to Eeb1p and Eht1p, has a minor role in medium-chain fatty acid ethyl ester biosynthesis; may be involved in lipid metabolism and detoxification Gene:YND1(YER005W)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Apyrase with wide substrate specificity, helps prevent inhibition of glycosylation by hydrolyzing nucleoside tri- and diphosphates that inhibit glycotransferases; partially redundant with Gda1p; mediates adenovirus E4orf4-induced toxicity Gene:YNL338W(YNL338W_d)|FD-Score:4.72|P-value:1.19E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps TEL14L-XC, which is Telomeric X element Core sequence on the left arm of Chromosome XIV Gene:YOL162W(YOL162W_p)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family Gene:YOR111W(YOR111W_p)|FD-Score:3.9|P-value:4.81E-5||SGD DESC:Putative protein of unknown function Gene:YPL039W(YPL039W_p)|FD-Score:-3.58|P-value:1.69E-4||SGD DESC:Putative protein of unknown function; YPL039W is not an essential gene Gene:YPL107W(YPL107W_p)|FD-Score:5.7|P-value:5.97E-9||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YPL107W is not an essential gene Gene:YPL108W(YPL108W_p)|FD-Score:-3.51|P-value:2.22E-4||SGD DESC:Cytoplasmic protein of unknown function; non-essential gene that is induced in a GDH1 deleted strain with altered redox metabolism; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YPT35(YHR105W)|FD-Score:-3.11|P-value:9.26E-4||SGD DESC:Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL045C6.086.04E-100.91FAD1Flavin adenine dinucleotide (FAD) synthetase, performs the second step in synthesis of FAD from riboflavin
YGR265W_d5.783.69E-90.91YGR265W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase
YLR275W4.885.42E-70.04SMD2Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2
YMR220W4.846.65E-70.33ERG8Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YFL039C4.513.27E-60.64ACT1Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions
YKL182W3.875.37E-50.00FAS1Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities
YJL097W3.875.46E-50.05PHS1Essential 3-hydroxyacyl-CoA dehydratase of the ER membrane, involved in elongation of very long-chain fatty acids; evolutionarily conserved, similar to mammalian PTPLA and PTPLB; involved in sphingolipid biosynthesis and protein trafficking
YBL097W3.826.79E-50.27BRN1Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission
YMR131C3.551.95E-40.03RRB1Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p
YPR176C3.522.18E-40.04BET2Beta subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p
YDL145C3.482.54E-40.01COP1Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway
YMR211W3.472.62E-40.03DML1Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
YOL010W3.442.89E-40.17RCL1Endonuclease that cleaves pre-rRNA at site A2 for 18S rRNA biogenesis; subunit of U3-containing 90S preribosome processome complex involved in small ribosomal subunit assembly; stimulates Bms1p GTPase and U3 binding activity; similar to RNA cyclase-like proteins but no cyclase activity detected
YOR335C3.275.44E-40.10ALA1Cytoplasmic and mitochondrial alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog
YDR288W3.177.68E-40.04NSE3Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKL222C7.341.10E-13YKL222CProtein of unknown function that may interact with ribosomes, based on co-purification experiments; similar to transcriptional regulators from the zinc cluster (binuclear cluster) family; null mutant is sensitive to caffeine
YBL060W7.212.87E-13YEL1Guanine nucleotide exchange factor specific for Arf3p; localized to the bud neck and tip; required for localization of Arf3p to the bud neck and tip
YPL107W_p5.705.97E-9YPL107W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YPL107W is not an essential gene
YKL130C5.501.95E-8SHE2RNA-binding protein that binds specific mRNAs and interacts with She3p; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud
YMR210W5.442.60E-8YMR210WPutative acyltransferase with similarity to Eeb1p and Eht1p, has a minor role in medium-chain fatty acid ethyl ester biosynthesis; may be involved in lipid metabolism and detoxification
YGR163W5.042.35E-7GTR2Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD
YML011C4.943.87E-7RAD33Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus
YNL338W_d4.721.19E-6YNL338W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps TEL14L-XC, which is Telomeric X element Core sequence on the left arm of Chromosome XIV
YKR019C4.611.97E-6IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
YGR053C_p4.582.29E-6YGR053C_pPutative protein of unknown function
YLR187W_p4.582.32E-6SKG3_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Skg4p; relocalizes from bud neck to cytoplasm upon DNA replication stress; SKG3 has a paralog, CAF120, that arose from the whole genome duplication
YJR103W4.513.32E-6URA8Minor CTP synthase isozyme (see also URA7); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA8 has a paralog, URA7, that arose from the whole genome duplication
YAL021C4.424.90E-6CCR4Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening
YOR252W4.405.49E-6TMA16Protein of unknown function that associates with ribosomes
YOL054W4.385.95E-6PSH1E3 ubiquitin ligase that mediates poyubiquitination and degradation of centromere-binding protein Cse4p and prevents Cse4p from mislocalizing to euchromatin; ubiquitylation of Cse4p may be antagonized by Scm3p

GO enrichment analysis for SGTC_2621
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0705.90E-8SGTC_2261,3-diallylurea 8.3 mMMiscellaneous745370.0615385
0.0552.14E-5SGTC_12044049-0315 18.3 μMChemDiv (Drug-like library)5884020.0243902
0.0517.78E-5SGTC_30359091303 49.5 μMChembridge (Drug-like library)252370020.0987654
0.0519.25E-5SGTC_266formosanin C 406.0 nMMiscellaneous735970.131148sphingolipid biosynthesis & PDR1
0.0501.26E-4SGTC_29429052777 62.2 μMChembridge (Drug-like library)64695030.0481928
0.0501.38E-4SGTC_29349036370 36.9 μMChembridge (Drug-like library)64628000.0631579
0.0491.67E-4SGTC_23499023730 187.1 μMChembridge (Fragment library)41262870.0779221
0.0491.72E-4SGTC_1904st060225 78.0 μMTimTec (Natural product derivative library)665950.0487805
0.0482.45E-4SGTC_9443370-0224 231.0 μMChemDiv (Drug-like library)45805140.0609756
0.0482.50E-4SGTC_10603448-7350 82.0 μMChemDiv (Drug-like library)5701780.0506329Golgi
0.0482.60E-4SGTC_28729044037 26.0 μMChembridge (Drug-like library)64651950.05
0.0472.86E-4SGTC_1678st019265 80.2 μMTimTec (Natural product derivative library)53670200.024390260S ribosome export
0.0463.52E-4SGTC_7171532-0246 73.2 μMChemDiv (Drug-like library)95619920.0540541Golgi
0.0454.91E-4SGTC_1121fusaric acid 630.5 nMTimTec (Natural product library)34420.106667
0.0455.24E-4SGTC_30989119039 49.5 μMChembridge (Drug-like library)171615280.0930233Golgi

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1560lithocholic acid53.1 μM0.6779669903TimTec (Pure natural product library)376.57265.18423
SGTC_2687chenodiol100 μM0.56255702181Microsource (Natural product library)392.5724.01434
SGTC_1517deoxycholic acid methyl ester49.2 μM0.514286229346TimTec (Pure natural product library)406.598584.30724
SGTC_2531methyl deoxycholate70.07 μM0.51428616667744Microsource (Natural product library)406.598584.30724
SGTC_2555cholic acid100 μM0.4266676708657Microsource (Natural product library)422.597983.13835
SGTC_2628lathosterol100 μM0.3513516710616Microsource (Natural product library)386.653547.37611
SGTC_1572epiandrosterone68.9 μM0.338235441302TimTec (Pure natural product library)290.44033.58812
SGTC_2540epiandrosterone100 μM0.338235441302TimTec (Pure natural product library)290.44033.58812
SGTC_2584androsterone48.74 μM0.3382352754130Microsource (Natural product library)290.44033.58812cell wall
SGTC_1974st06944826.9 μM0.229736323587TimTec (Natural product derivative library)305.454943.36923
SGTC_25303-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene92.62 μM0.2285716708760Microsource (Natural product library)216.31873.35611