3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2632
Screen concentration 100.0 μM
Source Microsource (Natural product library)
PubChem CID 6857784
SMILES CC1(CC2C1CCC3(CCC4C2(C3)OC(=O)CC4C(=O)O)C)C
Standardized SMILES CC12CCC3C(CC3(C)C)C4(C1)OC(=O)CC(C4CC2)C(=O)O
Molecular weight 320.4232
ALogP 3.15
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 0
% growth inhibition (Hom. pool) 1.18


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6857784
Download HIP data (tab-delimited text)  (excel)
Gene:BOS1(YLR078C)|FD-Score:4.7|P-value:1.31E-6|Clearance:1.08||SGD DESC:v-SNARE (vesicle specific SNAP receptor), localized to the endoplasmic reticulum membrane and necessary for vesicular transport from the ER to the Golgi Gene:CFT2(YLR115W)|FD-Score:3.44|P-value:2.89E-4|Clearance:0.33||SGD DESC:Subunit of the mRNA cleavage and polyadenlylation factor (CPF); required for pre-mRNA cleavage, polyadenylation and poly(A) site recognition, 43% similarity with the mammalian CPSF-100 protein. Gene:CKS1(YBR135W)|FD-Score:3.62|P-value:1.50E-4|Clearance:0.15||SGD DESC:Cyclin-dependent protein kinase regulatory subunit and adaptor; modulates proteolysis of M-phase targets through interactions with the proteasome; role in transcriptional regulation, recruiting proteasomal subunits to target gene promoters Gene:CWC24(YLR323C)|FD-Score:-3.11|P-value:9.26E-4|Clearance:0||SGD DESC:Spliceosome assembly factor, essential for the first step of splicing; component of the pre-catalytic spliceosome complex containing Cef1p; has similarity to S. pombe Cwf24p Gene:FRQ1(YDR373W)|FD-Score:-3.17|P-value:7.64E-4|Clearance:0||SGD DESC:N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily Gene:LCD1(YDR499W)|FD-Score:-3.11|P-value:9.41E-4|Clearance:0||SGD DESC:Essential protein required for the DNA integrity checkpoint pathways; interacts physically with Mec1p; putative homolog of S. pombe Rad26 and human ATRIP; forms nuclear foci upon DNA replication stress Gene:MES1(YGR264C)|FD-Score:-3.51|P-value:2.25E-4|Clearance:0||SGD DESC:Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs Gene:MMS21(YEL019C)|FD-Score:-3.48|P-value:2.55E-4|Clearance:0||SGD DESC:SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination Gene:NIC96(YFR002W)|FD-Score:4.97|P-value:3.40E-7|Clearance:1.08||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:PRE7(YBL041W)|FD-Score:3.11|P-value:9.38E-4|Clearance:0||SGD DESC:Beta 6 subunit of the 20S proteasome Gene:PRI2(YKL045W)|FD-Score:4.9|P-value:4.71E-7|Clearance:1.08||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:RFC4(YOL094C)|FD-Score:-3.72|P-value:1.00E-4|Clearance:0||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RIX1(YHR197W)|FD-Score:-3.83|P-value:6.48E-5|Clearance:0||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene Gene:RPN5(YDL147W)|FD-Score:-4.32|P-value:7.95E-6|Clearance:0||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:SPT5(YML010W)|FD-Score:-3.51|P-value:2.26E-4|Clearance:0||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair Gene:SUI1(YNL244C)|FD-Score:-3.71|P-value:1.03E-4|Clearance:0||SGD DESC:Translation initiation factor eIF1; component of a complex involved in recognition of the initiator codon; modulates translation accuracy at the initiation phase Gene:TFB4(YPR056W)|FD-Score:-3.5|P-value:2.33E-4|Clearance:0||SGD DESC:Subunit of TFIIH complex, involved in transcription initiation, similar to 34 kDa subunit of human TFIIH; interacts with Ssl1p Gene:UTP14(YML093W)|FD-Score:3.11|P-value:9.39E-4|Clearance:0.1||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:UTP25(YIL091C)|FD-Score:-3.58|P-value:1.69E-4|Clearance:0||SGD DESC:Nucleolar protein required for 35S pre-RNA processing and 40S ribosomal subunit biogenesis Gene:YBR190W(YBR190W_d)|FD-Score:3.47|P-value:2.63E-4|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W Gene:YRA1(YDR381W)|FD-Score:-3.57|P-value:1.82E-4|Clearance:0||SGD DESC:RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; functionally redundant with Yra2p, another REF family member Gene:BOS1(YLR078C)|FD-Score:4.7|P-value:1.31E-6|Clearance:1.08||SGD DESC:v-SNARE (vesicle specific SNAP receptor), localized to the endoplasmic reticulum membrane and necessary for vesicular transport from the ER to the Golgi Gene:CFT2(YLR115W)|FD-Score:3.44|P-value:2.89E-4|Clearance:0.33||SGD DESC:Subunit of the mRNA cleavage and polyadenlylation factor (CPF); required for pre-mRNA cleavage, polyadenylation and poly(A) site recognition, 43% similarity with the mammalian CPSF-100 protein. Gene:CKS1(YBR135W)|FD-Score:3.62|P-value:1.50E-4|Clearance:0.15||SGD DESC:Cyclin-dependent protein kinase regulatory subunit and adaptor; modulates proteolysis of M-phase targets through interactions with the proteasome; role in transcriptional regulation, recruiting proteasomal subunits to target gene promoters Gene:CWC24(YLR323C)|FD-Score:-3.11|P-value:9.26E-4|Clearance:0||SGD DESC:Spliceosome assembly factor, essential for the first step of splicing; component of the pre-catalytic spliceosome complex containing Cef1p; has similarity to S. pombe Cwf24p Gene:FRQ1(YDR373W)|FD-Score:-3.17|P-value:7.64E-4|Clearance:0||SGD DESC:N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily Gene:LCD1(YDR499W)|FD-Score:-3.11|P-value:9.41E-4|Clearance:0||SGD DESC:Essential protein required for the DNA integrity checkpoint pathways; interacts physically with Mec1p; putative homolog of S. pombe Rad26 and human ATRIP; forms nuclear foci upon DNA replication stress Gene:MES1(YGR264C)|FD-Score:-3.51|P-value:2.25E-4|Clearance:0||SGD DESC:Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs Gene:MMS21(YEL019C)|FD-Score:-3.48|P-value:2.55E-4|Clearance:0||SGD DESC:SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination Gene:NIC96(YFR002W)|FD-Score:4.97|P-value:3.40E-7|Clearance:1.08||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:PRE7(YBL041W)|FD-Score:3.11|P-value:9.38E-4|Clearance:0||SGD DESC:Beta 6 subunit of the 20S proteasome Gene:PRI2(YKL045W)|FD-Score:4.9|P-value:4.71E-7|Clearance:1.08||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:RFC4(YOL094C)|FD-Score:-3.72|P-value:1.00E-4|Clearance:0||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RIX1(YHR197W)|FD-Score:-3.83|P-value:6.48E-5|Clearance:0||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene Gene:RPN5(YDL147W)|FD-Score:-4.32|P-value:7.95E-6|Clearance:0||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:SPT5(YML010W)|FD-Score:-3.51|P-value:2.26E-4|Clearance:0||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair Gene:SUI1(YNL244C)|FD-Score:-3.71|P-value:1.03E-4|Clearance:0||SGD DESC:Translation initiation factor eIF1; component of a complex involved in recognition of the initiator codon; modulates translation accuracy at the initiation phase Gene:TFB4(YPR056W)|FD-Score:-3.5|P-value:2.33E-4|Clearance:0||SGD DESC:Subunit of TFIIH complex, involved in transcription initiation, similar to 34 kDa subunit of human TFIIH; interacts with Ssl1p Gene:UTP14(YML093W)|FD-Score:3.11|P-value:9.39E-4|Clearance:0.1||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:UTP25(YIL091C)|FD-Score:-3.58|P-value:1.69E-4|Clearance:0||SGD DESC:Nucleolar protein required for 35S pre-RNA processing and 40S ribosomal subunit biogenesis Gene:YBR190W(YBR190W_d)|FD-Score:3.47|P-value:2.63E-4|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W Gene:YRA1(YDR381W)|FD-Score:-3.57|P-value:1.82E-4|Clearance:0||SGD DESC:RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; functionally redundant with Yra2p, another REF family member

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6857784
Download HOP data (tab-delimited text)  (excel)
Gene:AIM32(YML050W)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:ARO1(YDR127W)|FD-Score:3.77|P-value:8.27E-5||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO7(YPR060C)|FD-Score:4.33|P-value:7.57E-6||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:ATG36(YJL185C)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:Pex3p interacting protein, required for pexophagy; interacts with Atg8p and Atg11p; mRNA is weakly cell cycle regulated, peaking in G2 phase; YJL185C is a non-essential gene Gene:BUD21(YOR078W)|FD-Score:-5.29|P-value:6.14E-8||SGD DESC:Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated as bud-site selection mutant that displays a random budding pattern Gene:CPA1(YOR303W)|FD-Score:-3.85|P-value:5.87E-5||SGD DESC:Small subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor; translationally regulated by an attenuator peptide encoded by YOR302W within the CPA1 mRNA 5'-leader Gene:CPD1(YGR247W)|FD-Score:4.05|P-value:2.60E-5||SGD DESC:Cyclic nucleotide phosphodiesterase; hydrolyzes ADP-ribose 1'', 2''-cyclic phosphate to ADP-ribose 1''-phosphate; may have a role in tRNA splicing; no detectable phenotype is conferred by null mutation or by overexpression; protein abundance increases in response to DNA replication stress Gene:CRT10(YOL063C)|FD-Score:-3.12|P-value:9.09E-4||SGD DESC:Protein involved in transcriptional regulation of RNR2 and RNR3; expression of the gene is induced by DNA damage and null mutations confer increased resistance to hydroxyurea; N-terminal region has a leucine repeat and a WD40 repeat Gene:DAL82(YNL314W)|FD-Score:3.19|P-value:7.03E-4||SGD DESC:Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain Gene:DFG5(YMR238W)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p Gene:DMA2(YNL116W)|FD-Score:4.66|P-value:1.62E-6||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma1p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ortholog of human RNF8 protein, with sequence similarity to human Chfr; contains FHA and RING finger domains Gene:EDC3(YEL015W)|FD-Score:-3.97|P-value:3.60E-5||SGD DESC:Non-essential conserved protein with a role in mRNA decapping; specifically affects the function of the decapping enzyme Dcp1p; localizes to cytoplasmic mRNA processing bodies; forms cytoplasmic foci upon DNA replication stress Gene:EHT1(YBR177C)|FD-Score:3.11|P-value:9.28E-4||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; plays a minor role in medium-chain fatty acid ethyl ester biosynthesis; possesses short-chain esterase activity; localizes to lipid particles and the mitochondrial outer membrane; EHT1 has a paralog, EEB1, that arose from the whole genome duplication Gene:ERV29(YGR284C)|FD-Score:-3.09|P-value:9.97E-4||SGD DESC:Protein localized to COPII-coated vesicles; involved in vesicle formation and incorporation of specific secretory cargo; protein abundance increases in response to DNA replication stress Gene:FIG4(YNL325C)|FD-Score:-5.9|P-value:1.80E-9||SGD DESC:Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain Gene:FMC1(YIL098C)|FD-Score:4.24|P-value:1.11E-5||SGD DESC:Mitochondrial matrix protein, required for assembly or stability at high temperature of the F1 sector of mitochondrial F1F0 ATP synthase; null mutant temperature sensitive growth on glycerol is suppressed by multicopy expression of Odc1p Gene:GAL83(YER027C)|FD-Score:-3.27|P-value:5.35E-4||SGD DESC:One of three possible beta-subunits of the Snf1 kinase complex, allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; contains glycogen-binding domain Gene:GRX4(YER174C)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx3p and Grx5p; protects cells from oxidative damage; mutant has increased aneuploidy tolerance; transcription is regulated by Yap5 Gene:HSP78(YDR258C)|FD-Score:-3.79|P-value:7.54E-5||SGD DESC:Oligomeric mitochondrial matrix chaperone that cooperates with Ssc1p in mitochondrial thermotolerance after heat shock; able to prevent the aggregation of misfolded proteins as well as resolubilize protein aggregates Gene:HXT14(YNL318C)|FD-Score:-3.14|P-value:8.53E-4||SGD DESC:Protein with similarity to hexose transporter family members, expression is induced in low glucose and repressed in high glucose; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:IME4(YGL192W)|FD-Score:5.8|P-value:3.33E-9||SGD DESC:Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids Gene:ISN1(YOR155C)|FD-Score:3.74|P-value:9.29E-5||SGD DESC:Inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase, catalyzes the breakdown of IMP to inosine, does not show similarity to known 5'-nucleotidases from other organisms Gene:LAG1(YHL003C)|FD-Score:3.22|P-value:6.33E-4||SGD DESC:Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress Gene:LEM3(YNL323W)|FD-Score:8.77|P-value:8.83E-19||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LOH1(YJL038C)|FD-Score:3.98|P-value:3.52E-5||SGD DESC:Protein of unknown function with proposed roles in maintenance of genome integrity and also in spore wall assembly; induced during sporulation; repressed during vegetative growth by Sum1p and Hst1p; sequence similar to IRC1 Gene:MDM31(YHR194W)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Mitochondrial protein that may have a role in phospholipid metabolism; inner membrane protein with similarity to Mdm32p; required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MMM2, MDM10, MDM12, and MDM34 Gene:MOS2(YGR235C)|FD-Score:9.29|P-value:7.41E-21||SGD DESC:Mitochondrial inner membrane protein; non-essential component of the mitochondrial inner membrane organizing system (MINOS, MitOS, or MICOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:MPA43(YNL249C)|FD-Score:6.3|P-value:1.48E-10||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:MRPL27(YBR282W)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; homolog of human Bcl-2 interacting protein BMRP Gene:MRPL6(YHR147C)|FD-Score:3.19|P-value:7.18E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSS18(YPR134W)|FD-Score:5.55|P-value:1.47E-8||SGD DESC:Nuclear encoded protein needed for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions Gene:MTC3(YGL226W)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1 Gene:NGR1(YBR212W)|FD-Score:-3.51|P-value:2.22E-4||SGD DESC:RNA binding protein that negatively regulates growth rate; interacts with the 3' UTR of the mitochondrial porin (POR1) mRNA and enhances its degradation; overexpression impairs mitochondrial function; interacts with Dhh1p to mediate POR1 mRNA decay; expressed in stationary phase Gene:NUR1(YDL089W)|FD-Score:5.42|P-value:2.91E-8||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:PAT1(YCR077C)|FD-Score:-3.58|P-value:1.75E-4||SGD DESC:Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress Gene:PEP3(YLR148W)|FD-Score:4.29|P-value:8.99E-6||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PHO3(YBR092C)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Constitutively expressed acid phosphatase similar to Pho5p; brought to the cell surface by transport vesicles; hydrolyzes thiamin phosphates in the periplasmic space, increasing cellular thiamin uptake; expression is repressed by thiamin Gene:POR2(YIL114C)|FD-Score:-6.81|P-value:4.98E-12||SGD DESC:Putative mitochondrial porin (voltage-dependent anion channel); not required for mitochondrial membrane permeability or mitochondrial osmotic stability; POR2 has a paralog, POR1, that arose from the whole genome duplication Gene:PRR1(YKL116C)|FD-Score:4.49|P-value:3.52E-6||SGD DESC:Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor Gene:PSY1(YKL076C_d)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C Gene:PTK1(YKL198C)|FD-Score:3.12|P-value:9.03E-4||SGD DESC:Putative serine/threonine protein kinase that regulates spermine uptake; involved in polyamine transport; possible mitochondrial protein Gene:RAD16(YBR114W)|FD-Score:5.03|P-value:2.39E-7||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:RCK2(YLR248W)|FD-Score:5.66|P-value:7.58E-9||SGD DESC:Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication Gene:RPL8A(YHL033C)|FD-Score:-4.72|P-value:1.18E-6||SGD DESC:Ribosomal 60S subunit protein L8A; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication Gene:RTT10(YPL183C)|FD-Score:-3.39|P-value:3.55E-4||SGD DESC:WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6 Gene:SAC6(YDR129C)|FD-Score:5.19|P-value:1.03E-7||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SKT5(YBL061C)|FD-Score:3.38|P-value:3.56E-4||SGD DESC:Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication Gene:SLX4(YLR135W)|FD-Score:-3.85|P-value:5.98E-5||SGD DESC:Endonuclease involved in processing DNA; acts during recombination and repair; cleaves branched structures in a complex with Slx1p; involved interstrand cross-link repair and in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during DSBR via single-strand annealing; relative distribution to nuclear foci increases upon DNA replication stress Gene:SRP40(YKR092C)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Nucleolar, serine-rich protein with a role in preribosome assembly or transport; may function as a chaperone of small nucleolar ribonucleoprotein particles (snoRNPs); immunologically and structurally to rat Nopp140 Gene:SWI4(YER111C)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p Gene:THO2(YNL139C)|FD-Score:3.13|P-value:8.60E-4||SGD DESC:Subunit of the THO complex, which is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids Gene:TRP3(YKL211C)|FD-Score:4.01|P-value:3.01E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TUM1(YOR251C)|FD-Score:-4.73|P-value:1.11E-6||SGD DESC:Rhodanese domain sulfur transferase, accepts persulfite from Nfs1p and transfers it to Uba4p in the pathway for 2-thiolation of the wobble uridine base of tRNAs; also stimulates sulfur transfer by Nfs1p; may be mitochondrially localized Gene:UBI4(YLL039C)|FD-Score:-4.3|P-value:8.42E-6||SGD DESC:Ubiquitin; becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-26S proteasome system; essential for the cellular stress response; encoded as a polyubiquitin precursor comprised of 5 head-to-tail repeats; protein abundance increases in response to DNA replication stress Gene:URE2(YNL229C)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VID22(YLR373C)|FD-Score:4.21|P-value:1.25E-5||SGD DESC:Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles Gene:VPH1(YOR270C)|FD-Score:4.39|P-value:5.67E-6||SGD DESC:Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress Gene:YBL071C(YBL071C_d)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Dubious open reading frame, predicted protein contains a peroxisomal targeting signal Gene:YBR062C(YBR062C_p)|FD-Score:-3.35|P-value:4.02E-4||SGD DESC:Protein of unknown function that interacts with Msb2p; may play a role in activation of the filamentous growth pathway. Gene:YBR134W(YBR134W_d)|FD-Score:-3.31|P-value:4.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YBR296C-A(YBR296C-A_p)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YCR016W(YCR016W_p)|FD-Score:-3.48|P-value:2.49E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis Gene:YER130C(YER130C_p)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress Gene:YFR012W-A(YFR012W-A_p)|FD-Score:4.33|P-value:7.47E-6||SGD DESC:Putative protein of unknown function; identified by homology Gene:YKL044W(YKL044W_p)|FD-Score:-3.24|P-value:5.91E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YKL097C(YKL097C_d)|FD-Score:-3.18|P-value:7.30E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species Gene:YMR074C(YMR074C)|FD-Score:-3.84|P-value:6.07E-5||SGD DESC:Protein with homology to human PDCD5; PDCD5 is involved in programmed cell death; N-terminal region forms a conserved triple-helix bundle structure; overexpression promotes H2O2-induced apoptosis; YMR074C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YMR084W(YMR084W_p)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Putative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YMR206W(YMR206W_p)|FD-Score:-3.11|P-value:9.37E-4||SGD DESC:Putative protein of unknown function; not an essential gene; YMR206W has a paralog, YNR014W, that arose from the whole genome duplication Gene:YNL146C-A(YNL146C-A_p)|FD-Score:3.1|P-value:9.70E-4||SGD DESC:Putative protein of unknown function Gene:YNR068C(YNR068C_p)|FD-Score:-4.02|P-value:2.89E-5||SGD DESC:Putative protein of unknown function; exhibits homology to C-terminal end of Bul1p; expressed as a readthrough product of BSC5, the readthrough locus being termed BUL3; the BUL3 readthrough product is involved in ubiquitin-mediated sorting of plasma membrane proteins and interacts with WW domains of Rsp5p in vitro, but in a functionally different way than the non-readthrough form Gene:YSW1(YBR148W)|FD-Score:3.8|P-value:7.16E-5||SGD DESC:Protein required for normal prospore membrane formation; interacts with Gip1p, which is the meiosis-specific regulatory subunit of the Glc7p protein phosphatase; expressed specifically in spores and localizes to the prospore membrane Gene:YTA12(YMR089C)|FD-Score:3.21|P-value:6.69E-4||SGD DESC:Component, with Afg3p, of the mitochondrial inner membrane m-AAA protease that mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes Gene:AIM32(YML050W)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:ARO1(YDR127W)|FD-Score:3.77|P-value:8.27E-5||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO7(YPR060C)|FD-Score:4.33|P-value:7.57E-6||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:ATG36(YJL185C)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:Pex3p interacting protein, required for pexophagy; interacts with Atg8p and Atg11p; mRNA is weakly cell cycle regulated, peaking in G2 phase; YJL185C is a non-essential gene Gene:BUD21(YOR078W)|FD-Score:-5.29|P-value:6.14E-8||SGD DESC:Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated as bud-site selection mutant that displays a random budding pattern Gene:CPA1(YOR303W)|FD-Score:-3.85|P-value:5.87E-5||SGD DESC:Small subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor; translationally regulated by an attenuator peptide encoded by YOR302W within the CPA1 mRNA 5'-leader Gene:CPD1(YGR247W)|FD-Score:4.05|P-value:2.60E-5||SGD DESC:Cyclic nucleotide phosphodiesterase; hydrolyzes ADP-ribose 1'', 2''-cyclic phosphate to ADP-ribose 1''-phosphate; may have a role in tRNA splicing; no detectable phenotype is conferred by null mutation or by overexpression; protein abundance increases in response to DNA replication stress Gene:CRT10(YOL063C)|FD-Score:-3.12|P-value:9.09E-4||SGD DESC:Protein involved in transcriptional regulation of RNR2 and RNR3; expression of the gene is induced by DNA damage and null mutations confer increased resistance to hydroxyurea; N-terminal region has a leucine repeat and a WD40 repeat Gene:DAL82(YNL314W)|FD-Score:3.19|P-value:7.03E-4||SGD DESC:Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain Gene:DFG5(YMR238W)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p Gene:DMA2(YNL116W)|FD-Score:4.66|P-value:1.62E-6||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma1p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ortholog of human RNF8 protein, with sequence similarity to human Chfr; contains FHA and RING finger domains Gene:EDC3(YEL015W)|FD-Score:-3.97|P-value:3.60E-5||SGD DESC:Non-essential conserved protein with a role in mRNA decapping; specifically affects the function of the decapping enzyme Dcp1p; localizes to cytoplasmic mRNA processing bodies; forms cytoplasmic foci upon DNA replication stress Gene:EHT1(YBR177C)|FD-Score:3.11|P-value:9.28E-4||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; plays a minor role in medium-chain fatty acid ethyl ester biosynthesis; possesses short-chain esterase activity; localizes to lipid particles and the mitochondrial outer membrane; EHT1 has a paralog, EEB1, that arose from the whole genome duplication Gene:ERV29(YGR284C)|FD-Score:-3.09|P-value:9.97E-4||SGD DESC:Protein localized to COPII-coated vesicles; involved in vesicle formation and incorporation of specific secretory cargo; protein abundance increases in response to DNA replication stress Gene:FIG4(YNL325C)|FD-Score:-5.9|P-value:1.80E-9||SGD DESC:Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain Gene:FMC1(YIL098C)|FD-Score:4.24|P-value:1.11E-5||SGD DESC:Mitochondrial matrix protein, required for assembly or stability at high temperature of the F1 sector of mitochondrial F1F0 ATP synthase; null mutant temperature sensitive growth on glycerol is suppressed by multicopy expression of Odc1p Gene:GAL83(YER027C)|FD-Score:-3.27|P-value:5.35E-4||SGD DESC:One of three possible beta-subunits of the Snf1 kinase complex, allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; contains glycogen-binding domain Gene:GRX4(YER174C)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx3p and Grx5p; protects cells from oxidative damage; mutant has increased aneuploidy tolerance; transcription is regulated by Yap5 Gene:HSP78(YDR258C)|FD-Score:-3.79|P-value:7.54E-5||SGD DESC:Oligomeric mitochondrial matrix chaperone that cooperates with Ssc1p in mitochondrial thermotolerance after heat shock; able to prevent the aggregation of misfolded proteins as well as resolubilize protein aggregates Gene:HXT14(YNL318C)|FD-Score:-3.14|P-value:8.53E-4||SGD DESC:Protein with similarity to hexose transporter family members, expression is induced in low glucose and repressed in high glucose; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:IME4(YGL192W)|FD-Score:5.8|P-value:3.33E-9||SGD DESC:Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids Gene:ISN1(YOR155C)|FD-Score:3.74|P-value:9.29E-5||SGD DESC:Inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase, catalyzes the breakdown of IMP to inosine, does not show similarity to known 5'-nucleotidases from other organisms Gene:LAG1(YHL003C)|FD-Score:3.22|P-value:6.33E-4||SGD DESC:Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress Gene:LEM3(YNL323W)|FD-Score:8.77|P-value:8.83E-19||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LOH1(YJL038C)|FD-Score:3.98|P-value:3.52E-5||SGD DESC:Protein of unknown function with proposed roles in maintenance of genome integrity and also in spore wall assembly; induced during sporulation; repressed during vegetative growth by Sum1p and Hst1p; sequence similar to IRC1 Gene:MDM31(YHR194W)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Mitochondrial protein that may have a role in phospholipid metabolism; inner membrane protein with similarity to Mdm32p; required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MMM2, MDM10, MDM12, and MDM34 Gene:MOS2(YGR235C)|FD-Score:9.29|P-value:7.41E-21||SGD DESC:Mitochondrial inner membrane protein; non-essential component of the mitochondrial inner membrane organizing system (MINOS, MitOS, or MICOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:MPA43(YNL249C)|FD-Score:6.3|P-value:1.48E-10||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:MRPL27(YBR282W)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; homolog of human Bcl-2 interacting protein BMRP Gene:MRPL6(YHR147C)|FD-Score:3.19|P-value:7.18E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSS18(YPR134W)|FD-Score:5.55|P-value:1.47E-8||SGD DESC:Nuclear encoded protein needed for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions Gene:MTC3(YGL226W)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1 Gene:NGR1(YBR212W)|FD-Score:-3.51|P-value:2.22E-4||SGD DESC:RNA binding protein that negatively regulates growth rate; interacts with the 3' UTR of the mitochondrial porin (POR1) mRNA and enhances its degradation; overexpression impairs mitochondrial function; interacts with Dhh1p to mediate POR1 mRNA decay; expressed in stationary phase Gene:NUR1(YDL089W)|FD-Score:5.42|P-value:2.91E-8||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:PAT1(YCR077C)|FD-Score:-3.58|P-value:1.75E-4||SGD DESC:Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress Gene:PEP3(YLR148W)|FD-Score:4.29|P-value:8.99E-6||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PHO3(YBR092C)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Constitutively expressed acid phosphatase similar to Pho5p; brought to the cell surface by transport vesicles; hydrolyzes thiamin phosphates in the periplasmic space, increasing cellular thiamin uptake; expression is repressed by thiamin Gene:POR2(YIL114C)|FD-Score:-6.81|P-value:4.98E-12||SGD DESC:Putative mitochondrial porin (voltage-dependent anion channel); not required for mitochondrial membrane permeability or mitochondrial osmotic stability; POR2 has a paralog, POR1, that arose from the whole genome duplication Gene:PRR1(YKL116C)|FD-Score:4.49|P-value:3.52E-6||SGD DESC:Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor Gene:PSY1(YKL076C_d)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C Gene:PTK1(YKL198C)|FD-Score:3.12|P-value:9.03E-4||SGD DESC:Putative serine/threonine protein kinase that regulates spermine uptake; involved in polyamine transport; possible mitochondrial protein Gene:RAD16(YBR114W)|FD-Score:5.03|P-value:2.39E-7||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:RCK2(YLR248W)|FD-Score:5.66|P-value:7.58E-9||SGD DESC:Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication Gene:RPL8A(YHL033C)|FD-Score:-4.72|P-value:1.18E-6||SGD DESC:Ribosomal 60S subunit protein L8A; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication Gene:RTT10(YPL183C)|FD-Score:-3.39|P-value:3.55E-4||SGD DESC:WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6 Gene:SAC6(YDR129C)|FD-Score:5.19|P-value:1.03E-7||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SKT5(YBL061C)|FD-Score:3.38|P-value:3.56E-4||SGD DESC:Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication Gene:SLX4(YLR135W)|FD-Score:-3.85|P-value:5.98E-5||SGD DESC:Endonuclease involved in processing DNA; acts during recombination and repair; cleaves branched structures in a complex with Slx1p; involved interstrand cross-link repair and in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during DSBR via single-strand annealing; relative distribution to nuclear foci increases upon DNA replication stress Gene:SRP40(YKR092C)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Nucleolar, serine-rich protein with a role in preribosome assembly or transport; may function as a chaperone of small nucleolar ribonucleoprotein particles (snoRNPs); immunologically and structurally to rat Nopp140 Gene:SWI4(YER111C)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p Gene:THO2(YNL139C)|FD-Score:3.13|P-value:8.60E-4||SGD DESC:Subunit of the THO complex, which is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids Gene:TRP3(YKL211C)|FD-Score:4.01|P-value:3.01E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TUM1(YOR251C)|FD-Score:-4.73|P-value:1.11E-6||SGD DESC:Rhodanese domain sulfur transferase, accepts persulfite from Nfs1p and transfers it to Uba4p in the pathway for 2-thiolation of the wobble uridine base of tRNAs; also stimulates sulfur transfer by Nfs1p; may be mitochondrially localized Gene:UBI4(YLL039C)|FD-Score:-4.3|P-value:8.42E-6||SGD DESC:Ubiquitin; becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-26S proteasome system; essential for the cellular stress response; encoded as a polyubiquitin precursor comprised of 5 head-to-tail repeats; protein abundance increases in response to DNA replication stress Gene:URE2(YNL229C)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VID22(YLR373C)|FD-Score:4.21|P-value:1.25E-5||SGD DESC:Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles Gene:VPH1(YOR270C)|FD-Score:4.39|P-value:5.67E-6||SGD DESC:Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress Gene:YBL071C(YBL071C_d)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Dubious open reading frame, predicted protein contains a peroxisomal targeting signal Gene:YBR062C(YBR062C_p)|FD-Score:-3.35|P-value:4.02E-4||SGD DESC:Protein of unknown function that interacts with Msb2p; may play a role in activation of the filamentous growth pathway. Gene:YBR134W(YBR134W_d)|FD-Score:-3.31|P-value:4.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YBR296C-A(YBR296C-A_p)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YCR016W(YCR016W_p)|FD-Score:-3.48|P-value:2.49E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis Gene:YER130C(YER130C_p)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress Gene:YFR012W-A(YFR012W-A_p)|FD-Score:4.33|P-value:7.47E-6||SGD DESC:Putative protein of unknown function; identified by homology Gene:YKL044W(YKL044W_p)|FD-Score:-3.24|P-value:5.91E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YKL097C(YKL097C_d)|FD-Score:-3.18|P-value:7.30E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species Gene:YMR074C(YMR074C)|FD-Score:-3.84|P-value:6.07E-5||SGD DESC:Protein with homology to human PDCD5; PDCD5 is involved in programmed cell death; N-terminal region forms a conserved triple-helix bundle structure; overexpression promotes H2O2-induced apoptosis; YMR074C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YMR084W(YMR084W_p)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Putative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YMR206W(YMR206W_p)|FD-Score:-3.11|P-value:9.37E-4||SGD DESC:Putative protein of unknown function; not an essential gene; YMR206W has a paralog, YNR014W, that arose from the whole genome duplication Gene:YNL146C-A(YNL146C-A_p)|FD-Score:3.1|P-value:9.70E-4||SGD DESC:Putative protein of unknown function Gene:YNR068C(YNR068C_p)|FD-Score:-4.02|P-value:2.89E-5||SGD DESC:Putative protein of unknown function; exhibits homology to C-terminal end of Bul1p; expressed as a readthrough product of BSC5, the readthrough locus being termed BUL3; the BUL3 readthrough product is involved in ubiquitin-mediated sorting of plasma membrane proteins and interacts with WW domains of Rsp5p in vitro, but in a functionally different way than the non-readthrough form Gene:YSW1(YBR148W)|FD-Score:3.8|P-value:7.16E-5||SGD DESC:Protein required for normal prospore membrane formation; interacts with Gip1p, which is the meiosis-specific regulatory subunit of the Glc7p protein phosphatase; expressed specifically in spores and localizes to the prospore membrane Gene:YTA12(YMR089C)|FD-Score:3.21|P-value:6.69E-4||SGD DESC:Component, with Afg3p, of the mitochondrial inner membrane m-AAA protease that mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YFR002W4.973.40E-71.08NIC96Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p)
YKL045W4.904.71E-71.08PRI2Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair
YLR078C4.701.31E-61.08BOS1v-SNARE (vesicle specific SNAP receptor), localized to the endoplasmic reticulum membrane and necessary for vesicular transport from the ER to the Golgi
YBR135W3.621.50E-40.15CKS1Cyclin-dependent protein kinase regulatory subunit and adaptor; modulates proteolysis of M-phase targets through interactions with the proteasome; role in transcriptional regulation, recruiting proteasomal subunits to target gene promoters
YBR190W_d3.472.63E-40.02YBR190W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W
YLR115W3.442.89E-40.33CFT2Subunit of the mRNA cleavage and polyadenlylation factor (CPF); required for pre-mRNA cleavage, polyadenylation and poly(A) site recognition, 43% similarity with the mammalian CPSF-100 protein.
YBL041W3.119.38E-45.11E-4PRE7Beta 6 subunit of the 20S proteasome
YML093W3.119.39E-40.10UTP14Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit
YDL195W3.010.001290.00SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YBR121C3.010.001310.03GRS1Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication
YGL091C2.970.001470.04NBP35Essential iron-sulfur cluster binding protein localized in the cytoplasm; forms a complex with Cfd1p that is involved in iron-sulfur protein assembly in the cytosol; similar to P-loop NTPases
YGL145W2.930.001670.04TIP20Peripheral membrane protein required for fusion of COPI vesicles with the ER; prohibits back-fusion of COPII vesicles with the ER; forms a tethering complex with Sec39p and Dsl1p that interacts with ER SNAREs Sec20p and Use1p
YCR057C2.900.001870.03PWP2Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis
YBR029C2.870.002040.04CDS1Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids
YOR103C2.830.002310.03OST2Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGR235C9.297.41E-21MOS2Mitochondrial inner membrane protein; non-essential component of the mitochondrial inner membrane organizing system (MINOS, MitOS, or MICOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture
YNL323W8.778.83E-19LEM3Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane
YNL249C6.301.48E-10MPA43Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YGL192W5.803.33E-9IME4Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids
YLR248W5.667.58E-9RCK2Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication
YPR134W5.551.47E-8MSS18Nuclear encoded protein needed for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions
YDL089W5.422.91E-8NUR1Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate
YDR129C5.191.03E-7SAC6Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress
YBR114W5.032.39E-7RAD16Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex
YNL116W4.661.62E-6DMA2Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma1p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ortholog of human RNF8 protein, with sequence similarity to human Chfr; contains FHA and RING finger domains
YKL116C4.493.52E-6PRR1Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor
YOR270C4.395.67E-6VPH1Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress
YFR012W-A_p4.337.47E-6YFR012W-A_pPutative protein of unknown function; identified by homology
YPR060C4.337.57E-6ARO7Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis
YLR148W4.298.99E-6PEP3Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis

GO enrichment analysis for SGTC_2632
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1163.37E-19SGTC_22547938975 95.2 μMChembridge (Fragment library)29692770.0555556
0.1111.19E-17SGTC_21325317594 122.7 μMChembridge (Fragment library)7945770.0131579SWF1 & branched chain AA biosynthesis
0.0945.21E-13SGTC_2636apigenin dimethyl ether 100.0 μMMicrosource (Natural product library)52816010.0833333
0.0921.18E-12SGTC_2607solasodine 47.4 μMMicrosource (Natural product library)67106420.152941
0.0849.84E-11SGTC_1656st012921 11.0 μMTimTec (Natural product derivative library)67328210.115942
0.0831.57E-10SGTC_1699st031318 29.1 μMTimTec (Natural product derivative library)53406580.0694444
0.0815.19E-10SGTC_21976588183 93.1 μMChembridge (Fragment library)8841360.0933333
0.0781.90E-9SGTC_2232chlorophetanol 41.5 μMMiscellaneous159070.0333333TRP & mitochondrial translation
0.0773.72E-9SGTC_5810958-0271 211.0 μMChemDiv (Drug-like library)45911450.0740741
0.0758.97E-9SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.105263
0.0759.23E-9SGTC_2618avocadyne 43.4 μMMicrosource (Natural product library)30151890.0153846
0.0741.18E-8SGTC_23579067248 200.0 μMChembridge (Fragment library)91502350.0909091
0.0715.44E-8SGTC_2170morpholine 200.0 μMChembridge (Fragment library)1211710.102941
0.0715.79E-8SGTC_458nppb 133.0 μMICCB bioactive library45490.0779221
0.0706.04E-8SGTC_2635hydroquinidine 100.0 μMMicrosource (Natural product library)4429120.0674157

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2631bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone100 μM0.4523847180Microsource (Natural product library)399.319283.40914
SGTC_2503beta-caryophyllene alcohol24.72 μM0.3861125Microsource (Natural product library)222.366343.42911
SGTC_25383',7'-epoxycaryophyllan-6-one100 μM0.3333334330530Microsource (Natural product library)236.349862.59902
SGTC_25422-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene100 μM0.2962965024745Microsource (Natural product library)238.365742.49912
SGTC_1125tnp002481.33 μM0.28070224207736TimTec (Natural product library)250.376443.49502
SGTC_2673kobusone100 μM0.2807026710676Microsource (Natural product library)222.323282.25102
SGTC_2558carylophyllene oxide100 μM0.2631586708694Microsource (Natural product library)206.323883.06301
SGTC_2650prasterone100 μM0.21538516759247Microsource (Natural product library)288.424423.33812
SGTC_2758dehydroepiandrosterone69.35 μM0.2153855881Miscellaneous288.424423.33812
SGTC_25005-alpha-androstan-3,17-dione61.44 μM0.2031256710655Microsource (Natural product library)288.424423.40202