Deletion Strain | FD score | P-value | Clearance | Gene | Gene Description |
---|
YKR081C | 6.06 | 6.79E-10 | 1.23 | RPF2 | Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles |
YMR235C | 4.84 | 6.66E-7 | 0.63 | RNA1 | GTPase activating protein (GAP) for Gsp1p, involved in nuclear transport |
YNL150W_d | 4.20 | 1.33E-5 | 0.07 | YNL150W_d | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking |
YPR016C | 4.13 | 1.78E-5 | 0.43 | TIF6 | Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits |
YOL120C | 3.70 | 1.06E-4 | 0.23 | RPL18A | Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication |
YLR175W | 3.47 | 2.57E-4 | 0.01 | CBF5 | Pseudouridine synthase catalytic subunit of box H/ACA small nucleolar ribonucleoprotein particles (snoRNPs), acts on both large and small rRNAs and on snRNA U2; mutations in human ortholog dyskerin cause the disorder dyskeratosis congenita |
YGL123W | 3.46 | 2.72E-4 | 0.23 | RPS2 | Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 |
YER127W | 3.23 | 6.24E-4 | 0.20 | LCP5 | Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus |
YBL020W | 3.03 | 0.00124 | 0.10 | RFT1 | Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein |
YGL137W | 2.92 | 0.00173 | 0.13 | SEC27 | Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP |
YGR083C | 2.79 | 0.00262 | 0.08 | GCD2 | Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression |
YFR028C | 2.71 | 0.00335 | 0.02 | CDC14 | Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR and Mitotic Exit Network in anaphase, enabling it to act on key substrates to effect a decrease in CDK/B-cyclin activity and mitotic exit; required for meiosis I spindle disassembly; released from nucleolus upon entry into anaphase I of meiosis, resequestered in metaphase II, then released again upon entry into anaphase II; maintained in nucleolus by Cdc55p in early meiosis |
YKL083W_d | 2.69 | 0.00356 | 0.05 | YKL083W_d | Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene RRP14 |
YOR294W | 2.64 | 0.00414 | 0.02 | RRS1 | Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice |
YDR013W | 2.62 | 0.00439 | 0.00 | PSF1 | Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery |