anisomycin

[(2R,3S,4S)-2-benzyl-4-hydroxypyrrolidin-3-yl] acetate

Antibiotic isolated from Streptomyces griseolus that inhibits protein synthesis. Acts by inhibiting peptidyl transferase activity in eukaryote ribosomes.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2649
Screen concentration 9.6 μM
Source ICCB bioactive library
PubChem CID 6610309
SMILES CC(=O)OC1C(CNC1CC2=CC=CC=C2)O
Standardized SMILES CC(=O)OC1C(O)CNC1Cc2ccccc2
Molecular weight 235.279
ALogP 0.89
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.76
% growth inhibition (Hom. pool) -1.18


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6610309
Download HIP data (tab-delimited text)  (excel)
Gene:ARP9(YMR033W)|FD-Score:-3.5|P-value:2.31E-4|Clearance:0||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CBF5(YLR175W)|FD-Score:3.47|P-value:2.57E-4|Clearance:0.01||SGD DESC:Pseudouridine synthase catalytic subunit of box H/ACA small nucleolar ribonucleoprotein particles (snoRNPs), acts on both large and small rRNAs and on snRNA U2; mutations in human ortholog dyskerin cause the disorder dyskeratosis congenita Gene:LCP5(YER127W)|FD-Score:3.23|P-value:6.24E-4|Clearance:0.2||SGD DESC:Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus Gene:RNA1(YMR235C)|FD-Score:4.84|P-value:6.66E-7|Clearance:0.63||SGD DESC:GTPase activating protein (GAP) for Gsp1p, involved in nuclear transport Gene:RPF2(YKR081C)|FD-Score:6.06|P-value:6.79E-10|Clearance:1.23||SGD DESC:Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles Gene:RPL18A(YOL120C)|FD-Score:3.7|P-value:1.06E-4|Clearance:0.23||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPS2(YGL123W)|FD-Score:3.46|P-value:2.72E-4|Clearance:0.23||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:TAF8(YML114C)|FD-Score:-3.62|P-value:1.49E-4|Clearance:0||SGD DESC:TFIID subunit (65 kDa), involved in RNA polymerase II transcription initiation Gene:TIF6(YPR016C)|FD-Score:4.13|P-value:1.78E-5|Clearance:0.43||SGD DESC:Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits Gene:YNL150W(YNL150W_d)|FD-Score:4.2|P-value:1.33E-5|Clearance:0.07||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking Gene:YOR203W(YOR203W_d)|FD-Score:-3.33|P-value:4.28E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps 5' end of essential DED1 gene required for translation initiation Gene:ARP9(YMR033W)|FD-Score:-3.5|P-value:2.31E-4|Clearance:0||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CBF5(YLR175W)|FD-Score:3.47|P-value:2.57E-4|Clearance:0.01||SGD DESC:Pseudouridine synthase catalytic subunit of box H/ACA small nucleolar ribonucleoprotein particles (snoRNPs), acts on both large and small rRNAs and on snRNA U2; mutations in human ortholog dyskerin cause the disorder dyskeratosis congenita Gene:LCP5(YER127W)|FD-Score:3.23|P-value:6.24E-4|Clearance:0.2||SGD DESC:Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus Gene:RNA1(YMR235C)|FD-Score:4.84|P-value:6.66E-7|Clearance:0.63||SGD DESC:GTPase activating protein (GAP) for Gsp1p, involved in nuclear transport Gene:RPF2(YKR081C)|FD-Score:6.06|P-value:6.79E-10|Clearance:1.23||SGD DESC:Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles Gene:RPL18A(YOL120C)|FD-Score:3.7|P-value:1.06E-4|Clearance:0.23||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPS2(YGL123W)|FD-Score:3.46|P-value:2.72E-4|Clearance:0.23||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:TAF8(YML114C)|FD-Score:-3.62|P-value:1.49E-4|Clearance:0||SGD DESC:TFIID subunit (65 kDa), involved in RNA polymerase II transcription initiation Gene:TIF6(YPR016C)|FD-Score:4.13|P-value:1.78E-5|Clearance:0.43||SGD DESC:Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits Gene:YNL150W(YNL150W_d)|FD-Score:4.2|P-value:1.33E-5|Clearance:0.07||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking Gene:YOR203W(YOR203W_d)|FD-Score:-3.33|P-value:4.28E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps 5' end of essential DED1 gene required for translation initiation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6610309
Download HOP data (tab-delimited text)  (excel)
Gene:ATG17(YLR423C)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells Gene:BNA1(YJR025C)|FD-Score:-3.24|P-value:5.93E-4||SGD DESC:3-hydroxyanthranilic acid dioxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:CMC1(YKL137W)|FD-Score:3.37|P-value:3.79E-4||SGD DESC:Evolutionarily conserved copper-binding protein of the mitochondrial intermembrane space, may be involved in delivering copper from the matrix to the cytochrome c oxidase complex; contains a twin CX9C motif Gene:CSR2(YPR030W)|FD-Score:3.81|P-value:6.86E-5||SGD DESC:Nuclear ubiquitin protein ligase binding protein; may regulate utilization of nonfermentable carbon sources and endocytosis of plasma membrane proteins; overproduction suppresses chs5 spa2 lethality at high temp; ubiquitinated by Rsp5p, deubiquitinated by Ubp2p; CSR2 has a paralog, ECM21, that arose from the whole genome duplication Gene:DBF2(YGR092W)|FD-Score:3.74|P-value:9.32E-5||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DTD1(YDL219W)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:D-Tyr-tRNA(Tyr) deacylase, functions in protein translation, may affect nonsense suppression via alteration of the protein synthesis machinery; ubiquitous among eukaryotes Gene:ECM7(YLR443W)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Non-essential putative integral membrane protein with a role in calcium uptake; mutant has cell wall defects and Ca+ uptake deficiencies; transcription is induced under conditions of zinc deficiency Gene:ERG5(YMR015C)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:HIS5(YIL116W)|FD-Score:3.21|P-value:6.56E-4||SGD DESC:Histidinol-phosphate aminotransferase, catalyzes the seventh step in histidine biosynthesis; responsive to general control of amino acid biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts Gene:ISR1(YPR106W)|FD-Score:-3.11|P-value:9.45E-4||SGD DESC:Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C Gene:KEL2(YGR238C)|FD-Score:3.82|P-value:6.68E-5||SGD DESC:Protein that functions in a complex with Kel1p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate Gene:LEE1(YPL054W)|FD-Score:4.43|P-value:4.69E-6||SGD DESC:Zinc-finger protein of unknown function Gene:LIP2(YLR239C)|FD-Score:3.81|P-value:6.92E-5||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:MMS1(YPR164W)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; regulates Ty1 transposition; involved with Rtt101p in nonfunctional rRNA decay Gene:MNL1(YHR204W)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; in complex with Pdi1p, generates a Man7GlcNac2 oligosaccharide signal on glycoproteins destined for ubiquitin-proteasome degradation Gene:MSG5(YNL053W)|FD-Score:-3.76|P-value:8.67E-5||SGD DESC:Dual-specificity protein phosphatase; exists in 2 isoforms; required for maintenance of a low level of signaling through the cell integrity pathway, adaptive response to pheromone; regulates and is regulated by Slt2p; dephosphorylates Fus3p Gene:OCA5(YHL029C_p)|FD-Score:7.88|P-value:1.68E-15||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:PAN5(YHR063C)|FD-Score:5.02|P-value:2.63E-7||SGD DESC:2-dehydropantoate 2-reductase, part of the pantothenic acid pathway, structurally homologous to E. coli panE Gene:PDR1(YGL013C)|FD-Score:3.24|P-value:6.00E-4||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:POL4(YCR014C)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:DNA polymerase IV, undergoes pair-wise interactions with Dnl4p-Lif1p and Rad27p to mediate repair of DNA double-strand breaks by non-homologous end joining (NHEJ); homologous to mammalian DNA polymerase beta Gene:SER33(YIL074C)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER33 has a paralog, SER3, that arose from the whole genome duplication Gene:SHE9(YDR393W)|FD-Score:3.24|P-value:5.91E-4||SGD DESC:Mitochondrial inner membrane protein required for normal mitochondrial morphology, may be involved in fission of the inner membrane; forms a homo-oligomeric complex Gene:SIA1(YOR137C)|FD-Score:3.14|P-value:8.53E-4||SGD DESC:Protein of unassigned function involved in activation of the Pma1p plasma membrane H+-ATPase by glucose Gene:SSN2(YDR443C)|FD-Score:-3.1|P-value:9.81E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:SYP1(YCR030C)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Protein of unknown function that is involved in endocytic site formation; may regulate assembly and disassembly of the septin ring; colocalizes and interacts with septin subunits; potential role in actin cytoskeletal organization Gene:TRE1(YPL176C)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:UBP9(YER098W)|FD-Score:4.8|P-value:8.00E-7||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin-protein fusions; UBP9 has a paralog, UBP13, that arose from the whole genome duplication Gene:YHR125W(YHR125W_d)|FD-Score:4.34|P-value:7.09E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR171W(YLR171W_d)|FD-Score:3.21|P-value:6.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR412C-A(YLR412C-A_p)|FD-Score:3.26|P-value:5.61E-4||SGD DESC:Putative protein of unknown function Gene:YNL228W(YNL228W_d)|FD-Score:4.51|P-value:3.19E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF YNL227C/JJJ1 Gene:YPR170C(YPR170C_d)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B Gene:ATG17(YLR423C)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells Gene:BNA1(YJR025C)|FD-Score:-3.24|P-value:5.93E-4||SGD DESC:3-hydroxyanthranilic acid dioxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:CMC1(YKL137W)|FD-Score:3.37|P-value:3.79E-4||SGD DESC:Evolutionarily conserved copper-binding protein of the mitochondrial intermembrane space, may be involved in delivering copper from the matrix to the cytochrome c oxidase complex; contains a twin CX9C motif Gene:CSR2(YPR030W)|FD-Score:3.81|P-value:6.86E-5||SGD DESC:Nuclear ubiquitin protein ligase binding protein; may regulate utilization of nonfermentable carbon sources and endocytosis of plasma membrane proteins; overproduction suppresses chs5 spa2 lethality at high temp; ubiquitinated by Rsp5p, deubiquitinated by Ubp2p; CSR2 has a paralog, ECM21, that arose from the whole genome duplication Gene:DBF2(YGR092W)|FD-Score:3.74|P-value:9.32E-5||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DTD1(YDL219W)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:D-Tyr-tRNA(Tyr) deacylase, functions in protein translation, may affect nonsense suppression via alteration of the protein synthesis machinery; ubiquitous among eukaryotes Gene:ECM7(YLR443W)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Non-essential putative integral membrane protein with a role in calcium uptake; mutant has cell wall defects and Ca+ uptake deficiencies; transcription is induced under conditions of zinc deficiency Gene:ERG5(YMR015C)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:HIS5(YIL116W)|FD-Score:3.21|P-value:6.56E-4||SGD DESC:Histidinol-phosphate aminotransferase, catalyzes the seventh step in histidine biosynthesis; responsive to general control of amino acid biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts Gene:ISR1(YPR106W)|FD-Score:-3.11|P-value:9.45E-4||SGD DESC:Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C Gene:KEL2(YGR238C)|FD-Score:3.82|P-value:6.68E-5||SGD DESC:Protein that functions in a complex with Kel1p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate Gene:LEE1(YPL054W)|FD-Score:4.43|P-value:4.69E-6||SGD DESC:Zinc-finger protein of unknown function Gene:LIP2(YLR239C)|FD-Score:3.81|P-value:6.92E-5||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:MMS1(YPR164W)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; regulates Ty1 transposition; involved with Rtt101p in nonfunctional rRNA decay Gene:MNL1(YHR204W)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; in complex with Pdi1p, generates a Man7GlcNac2 oligosaccharide signal on glycoproteins destined for ubiquitin-proteasome degradation Gene:MSG5(YNL053W)|FD-Score:-3.76|P-value:8.67E-5||SGD DESC:Dual-specificity protein phosphatase; exists in 2 isoforms; required for maintenance of a low level of signaling through the cell integrity pathway, adaptive response to pheromone; regulates and is regulated by Slt2p; dephosphorylates Fus3p Gene:OCA5(YHL029C_p)|FD-Score:7.88|P-value:1.68E-15||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:PAN5(YHR063C)|FD-Score:5.02|P-value:2.63E-7||SGD DESC:2-dehydropantoate 2-reductase, part of the pantothenic acid pathway, structurally homologous to E. coli panE Gene:PDR1(YGL013C)|FD-Score:3.24|P-value:6.00E-4||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:POL4(YCR014C)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:DNA polymerase IV, undergoes pair-wise interactions with Dnl4p-Lif1p and Rad27p to mediate repair of DNA double-strand breaks by non-homologous end joining (NHEJ); homologous to mammalian DNA polymerase beta Gene:SER33(YIL074C)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER33 has a paralog, SER3, that arose from the whole genome duplication Gene:SHE9(YDR393W)|FD-Score:3.24|P-value:5.91E-4||SGD DESC:Mitochondrial inner membrane protein required for normal mitochondrial morphology, may be involved in fission of the inner membrane; forms a homo-oligomeric complex Gene:SIA1(YOR137C)|FD-Score:3.14|P-value:8.53E-4||SGD DESC:Protein of unassigned function involved in activation of the Pma1p plasma membrane H+-ATPase by glucose Gene:SSN2(YDR443C)|FD-Score:-3.1|P-value:9.81E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:SYP1(YCR030C)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Protein of unknown function that is involved in endocytic site formation; may regulate assembly and disassembly of the septin ring; colocalizes and interacts with septin subunits; potential role in actin cytoskeletal organization Gene:TRE1(YPL176C)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:UBP9(YER098W)|FD-Score:4.8|P-value:8.00E-7||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin-protein fusions; UBP9 has a paralog, UBP13, that arose from the whole genome duplication Gene:YHR125W(YHR125W_d)|FD-Score:4.34|P-value:7.09E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR171W(YLR171W_d)|FD-Score:3.21|P-value:6.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR412C-A(YLR412C-A_p)|FD-Score:3.26|P-value:5.61E-4||SGD DESC:Putative protein of unknown function Gene:YNL228W(YNL228W_d)|FD-Score:4.51|P-value:3.19E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF YNL227C/JJJ1 Gene:YPR170C(YPR170C_d)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YKR081C6.066.79E-101.23RPF2Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles
YMR235C4.846.66E-70.63RNA1GTPase activating protein (GAP) for Gsp1p, involved in nuclear transport
YNL150W_d4.201.33E-50.07YNL150W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking
YPR016C4.131.78E-50.43TIF6Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits
YOL120C3.701.06E-40.23RPL18ARibosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication
YLR175W3.472.57E-40.01CBF5Pseudouridine synthase catalytic subunit of box H/ACA small nucleolar ribonucleoprotein particles (snoRNPs), acts on both large and small rRNAs and on snRNA U2; mutations in human ortholog dyskerin cause the disorder dyskeratosis congenita
YGL123W3.462.72E-40.23RPS2Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5
YER127W3.236.24E-40.20LCP5Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus
YBL020W3.030.001240.10RFT1Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein
YGL137W2.920.001730.13SEC27Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP
YGR083C2.790.002620.08GCD2Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YFR028C2.710.003350.02CDC14Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR and Mitotic Exit Network in anaphase, enabling it to act on key substrates to effect a decrease in CDK/B-cyclin activity and mitotic exit; required for meiosis I spindle disassembly; released from nucleolus upon entry into anaphase I of meiosis, resequestered in metaphase II, then released again upon entry into anaphase II; maintained in nucleolus by Cdc55p in early meiosis
YKL083W_d2.690.003560.05YKL083W_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene RRP14
YOR294W2.640.004140.02RRS1Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice
YDR013W2.620.004390.00PSF1Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YHL029C_p7.881.68E-15OCA5_pCytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts
YHR063C5.022.63E-7PAN52-dehydropantoate 2-reductase, part of the pantothenic acid pathway, structurally homologous to E. coli panE
YER098W4.808.00E-7UBP9Ubiquitin-specific protease that cleaves ubiquitin-protein fusions; UBP9 has a paralog, UBP13, that arose from the whole genome duplication
YNL228W_d4.513.19E-6YNL228W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF YNL227C/JJJ1
YPL054W4.434.69E-6LEE1Zinc-finger protein of unknown function
YHR125W_d4.347.09E-6YHR125W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YGR238C3.826.68E-5KEL2Protein that functions in a complex with Kel1p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate
YPR030W3.816.86E-5CSR2Nuclear ubiquitin protein ligase binding protein; may regulate utilization of nonfermentable carbon sources and endocytosis of plasma membrane proteins; overproduction suppresses chs5 spa2 lethality at high temp; ubiquitinated by Rsp5p, deubiquitinated by Ubp2p; CSR2 has a paralog, ECM21, that arose from the whole genome duplication
YLR239C3.816.92E-5LIP2Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups
YGR092W3.749.32E-5DBF2Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis
YPR164W3.691.13E-4MMS1Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; regulates Ty1 transposition; involved with Rtt101p in nonfunctional rRNA decay
YCR014C3.681.15E-4POL4DNA polymerase IV, undergoes pair-wise interactions with Dnl4p-Lif1p and Rad27p to mediate repair of DNA double-strand breaks by non-homologous end joining (NHEJ); homologous to mammalian DNA polymerase beta
YLR423C3.601.59E-4ATG17Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells
YPR170C_d3.591.65E-4YPR170C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B
YCR030C3.561.83E-4SYP1Protein of unknown function that is involved in endocytic site formation; may regulate assembly and disassembly of the septin ring; colocalizes and interacts with septin subunits; potential role in actin cytoskeletal organization

GO enrichment analysis for SGTC_2649
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0905.33E-12SGTC_2234tolnaftate 200.0 μMMiscellaneous55100.121212
0.0895.84E-12SGTC_32419133421 49.5 μMChembridge (Drug-like library)170182210.0952381PDR1
0.0871.93E-11SGTC_295k015-0027 31.0 μMChemDiv (Drug-like library)38874240.101449PDR1
0.0781.74E-9SGTC_12430416-0003 5.7 μMChemDiv (Drug-like library)21419770.0877193PDR1
0.0782.14E-9SGTC_8890919-1524 82.7 μMChemDiv (Drug-like library)39054520.147059
0.0772.85E-9SGTC_12770873-0036 22.0 μMChemDiv (Drug-like library)59657350.101449PDR1
0.0741.28E-8SGTC_5274476-4215 268.0 μMChemDiv (Drug-like library)44187540.114943PDR1
0.0714.57E-8SGTC_687k029-0044 34.9 μMChemDiv (Drug-like library)58293380.0512821PDR1
0.0681.90E-7SGTC_180111-0177 500.0 nMChemDiv (Drug-like library)7217520.0943396
0.0664.30E-7SGTC_1694st024011 44.9 μMTimTec (Natural product derivative library)27875330.139241ERG2
0.0664.71E-7SGTC_8430310-0051 143.0 μMChemDiv (Drug-like library)20650370.102941
0.0656.41E-7SGTC_20585236698 133.0 μMChembridge (Fragment library)7666650.133333
0.0621.63E-6SGTC_5850kpi-0023 191.0 μMChemDiv (Drug-like library)646010.15
0.0603.80E-6SGTC_30469092212 49.5 μMChembridge (Drug-like library)252366370.181818
0.0604.23E-6SGTC_460329-0187a 2.2 μMChemDiv (Drug-like library)464948170.111111

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_395anisomycin6.25 μM16610309ICCB bioactive library235.278980.89324
SGTC_415dibenzyl ketone100 μM0.2857147593Miscellaneous210.271063.29401
SGTC_2007401153971.82 μM0.27777845595301Chembridge (Fragment library)285.180181.42712cell wall signaling
SGTC_1235benzylparaben21.5 μM0.274517180ChemDiv (Drug-like library)228.243283.02613
SGTC_7420kpi-0133140 μM0.2586212728852ChemDiv (Drug-like library)297.434483.98912ERG2
SGTC_10472043-304482 μM0.253968669592ChemDiv (Drug-like library)323.385643.66903
SGTC_2848901637025.97 μM0.2537317894410Chembridge (Drug-like library)353.411623.50404
SGTC_2721ifenprodil61.44 μM0.2459023689Miscellaneous325.444584.18523plasma membrane duress
SGTC_3531988-117558.3 μM0.2413792788357ChemDiv (Drug-like library)269.29522.39413unfolded protein response
SGTC_6164092-0450108 μM0.241379787165ChemDiv (Drug-like library)322.443943.76202