mycophenolic acid FDA approved compound

(E)-6-(4-hydroxy-6-methoxy-7-methyl-3-oxo-1H-2-benzofuran-5-yl)-4-methylhex-4-enoic acid

Mycophenolic acid is a potent reversible inhibitor of IMPDH (inosine monophosphate dehydrogenase), leading to a depletion of GMP and inhibition of de novo synthesis of purines. The resulting imbalance of nucleotide pools also results in inhibiting transcription-elongation.

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 

PubChem MeSH terms: Antibiotics, Antineoplastic;Enzyme Inhibitors



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_265
Screen concentration 32.9 μM
Source Miscellaneous
PubChem CID 446541
SMILES CC1=C(C(=C(C2=C1COC2=O)O)CC=C(C)CCC(=O)O)OC
Standardized SMILES COc1c(C)c2COC(=O)c2c(O)c1CC=C(C)CCC(=O)O
Molecular weight 320.3371
ALogP 3.16
H-bond donor count 2
H-bond acceptor count 6
Response signature RPP1 & pyrimidine depletion

Pool Growth Kinetics
% growth inhibition (Het. pool) 4.65
% growth inhibition (Hom. pool) 2.38


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 446541
Download HIP data (tab-delimited text)  (excel)
Gene:BPL1(YDL141W)|FD-Score:4.59|P-value:2.19E-6|Clearance:0.14||SGD DESC:Biotin:apoprotein ligase, covalently modifies proteins with the addition of biotin, required for acetyl-CoA carboxylase (Acc1p) holoenzyme formation Gene:DAD2(YKR083C)|FD-Score:3.59|P-value:1.67E-4|Clearance:0.28||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:DED1(YOR204W)|FD-Score:4.45|P-value:4.35E-6|Clearance:0.24||SGD DESC:ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility Gene:ESS1(YJR017C)|FD-Score:3.25|P-value:5.76E-4|Clearance:0.1||SGD DESC:Peptidylprolyl-cis/trans-isomerase (PPIase); specific for phosphorylated serine and threonine residues N-terminal to proline; regulates phosphorylation of the RNAP II large subunit (Rpo21p) C-terminal domain (CTD); associates with phospho-Ser5 form of RNAP II in vivo; regulates phosphorylation of Ser7 within CTD; present along entire coding length of genes; represses initiation of CUTs; required for efficient termination of mRNA transcription and trimethylation of histone H3 Gene:GPI13(YLL031C)|FD-Score:-3.36|P-value:3.87E-4|Clearance:0||SGD DESC:ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein Gene:POP1(YNL221C)|FD-Score:4.11|P-value:1.94E-5|Clearance:0.53||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:PRP4(YPR178W)|FD-Score:5.23|P-value:8.47E-8|Clearance:0.64||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RAP1(YNL216W)|FD-Score:3.31|P-value:4.62E-4|Clearance:0.06||SGD DESC:Essential DNA-binding transcription regulator that binds at many loci; involved in either transcription activation or repression, chromatin silencing, and telomere length maintenance; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain) Gene:RPP1(YHR062C)|FD-Score:18.6|P-value:1.63E-77|Clearance:13.37||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SNU23(YDL098C)|FD-Score:-3.42|P-value:3.12E-4|Clearance:0||SGD DESC:Component of U4/U6.U5 snRNP involved in mRNA splicing via spliceosome Gene:SPC98(YNL126W)|FD-Score:4.21|P-value:1.27E-5|Clearance:0.1||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:YPI1(YFR003C)|FD-Score:3.16|P-value:8.02E-4|Clearance:0.13||SGD DESC:Regulatory subunit of the type I protein phosphatase (PP1) Glc7p; Glc7p participates in the regulation of a variety of metabolic processes including mitosis and glycogen metabolism; in vitro evidence suggests Ypi1p is an inhibitor of Glc7p while in vivo evidence suggests it is an activator; overproduction causes decreased cellular content of glycogen; partial depletion causes lithium sensitivity, while overproduction confers lithium-tolerance Gene:BPL1(YDL141W)|FD-Score:4.59|P-value:2.19E-6|Clearance:0.14||SGD DESC:Biotin:apoprotein ligase, covalently modifies proteins with the addition of biotin, required for acetyl-CoA carboxylase (Acc1p) holoenzyme formation Gene:DAD2(YKR083C)|FD-Score:3.59|P-value:1.67E-4|Clearance:0.28||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:DED1(YOR204W)|FD-Score:4.45|P-value:4.35E-6|Clearance:0.24||SGD DESC:ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility Gene:ESS1(YJR017C)|FD-Score:3.25|P-value:5.76E-4|Clearance:0.1||SGD DESC:Peptidylprolyl-cis/trans-isomerase (PPIase); specific for phosphorylated serine and threonine residues N-terminal to proline; regulates phosphorylation of the RNAP II large subunit (Rpo21p) C-terminal domain (CTD); associates with phospho-Ser5 form of RNAP II in vivo; regulates phosphorylation of Ser7 within CTD; present along entire coding length of genes; represses initiation of CUTs; required for efficient termination of mRNA transcription and trimethylation of histone H3 Gene:GPI13(YLL031C)|FD-Score:-3.36|P-value:3.87E-4|Clearance:0||SGD DESC:ER membrane localized phosphoryltransferase that adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein Gene:POP1(YNL221C)|FD-Score:4.11|P-value:1.94E-5|Clearance:0.53||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:PRP4(YPR178W)|FD-Score:5.23|P-value:8.47E-8|Clearance:0.64||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RAP1(YNL216W)|FD-Score:3.31|P-value:4.62E-4|Clearance:0.06||SGD DESC:Essential DNA-binding transcription regulator that binds at many loci; involved in either transcription activation or repression, chromatin silencing, and telomere length maintenance; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain) Gene:RPP1(YHR062C)|FD-Score:18.6|P-value:1.63E-77|Clearance:13.37||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SNU23(YDL098C)|FD-Score:-3.42|P-value:3.12E-4|Clearance:0||SGD DESC:Component of U4/U6.U5 snRNP involved in mRNA splicing via spliceosome Gene:SPC98(YNL126W)|FD-Score:4.21|P-value:1.27E-5|Clearance:0.1||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:YPI1(YFR003C)|FD-Score:3.16|P-value:8.02E-4|Clearance:0.13||SGD DESC:Regulatory subunit of the type I protein phosphatase (PP1) Glc7p; Glc7p participates in the regulation of a variety of metabolic processes including mitosis and glycogen metabolism; in vitro evidence suggests Ypi1p is an inhibitor of Glc7p while in vivo evidence suggests it is an activator; overproduction causes decreased cellular content of glycogen; partial depletion causes lithium sensitivity, while overproduction confers lithium-tolerance

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 446541
Download HOP data (tab-delimited text)  (excel)
Gene:BGL2(YGR282C)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Endo-beta-1,3-glucanase, major protein of the cell wall, involved in cell wall maintenance Gene:CAJ1(YER048C)|FD-Score:4.12|P-value:1.93E-5||SGD DESC:Nuclear type II J heat shock protein of the E. coli dnaJ family, contains a leucine zipper-like motif, binds to non-native substrates for presentation to Ssa3p, may function during protein translocation, assembly and disassembly Gene:CBC2(YPL178W)|FD-Score:-3.18|P-value:7.27E-4||SGD DESC:Small subunit of the heterodimeric cap binding complex that also contains Sto1p, component of the spliceosomal commitment complex; interacts with Npl3p, possibly to package mRNA for export from the nucleus; contains an RNA-binding motif Gene:CCW12(YLR110C)|FD-Score:3.93|P-value:4.34E-5||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CLC1(YGR167W)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:COG6(YNL041C)|FD-Score:5.36|P-value:4.10E-8||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DAP1(YPL170W)|FD-Score:3.14|P-value:8.42E-4||SGD DESC:Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis Gene:DOC1(YGL240W)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:Processivity factor required for the ubiquitination activity of the anaphase promoting complex (APC), mediates the activity of the APC by contributing to substrate recognition; involved in cyclin proteolysis; contains a conserved DOC1 homology domain Gene:ERV29(YGR284C)|FD-Score:4.7|P-value:1.29E-6||SGD DESC:Protein localized to COPII-coated vesicles; involved in vesicle formation and incorporation of specific secretory cargo; protein abundance increases in response to DNA replication stress Gene:FRE3(YOR381W)|FD-Score:3.3|P-value:4.86E-4||SGD DESC:Ferric reductase, reduces siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels Gene:FRE5(YOR384W)|FD-Score:3.85|P-value:5.95E-5||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FRK1(YPL141C)|FD-Score:4.26|P-value:1.00E-5||SGD DESC:Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; interacts with rRNA transcription and ribosome biogenesis factors and the long chain fatty acyl-CoA synthetase Faa3p; FRK1 has a paralog, KIN4, that arose from the whole genome duplication Gene:FUS2(YMR232W)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Cytoplasmic protein localized to the shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating Gene:GIR2(YDR152W)|FD-Score:3.74|P-value:9.12E-5||SGD DESC:Highly-acidic cytoplasmic RWD domain-containing protein of unknown function; forms a complex with Rbg2p; interacts with Rbg1p and Gcn1p; associates with translating ribosomes; putative intrinsically unstructured protein Gene:GLO1(YML004C)|FD-Score:4.02|P-value:2.92E-5||SGD DESC:Monomeric glyoxalase I, catalyzes the detoxification of methylglyoxal (a by-product of glycolysis) via condensation with glutathione to produce S-D-lactoylglutathione; expression regulated by methylglyoxal levels and osmotic stress Gene:GTO3(YMR251W)|FD-Score:3.85|P-value:5.87E-5||SGD DESC:Omega class glutathione transferase; putative cytosolic localization Gene:HGH1(YGR187C)|FD-Score:3.16|P-value:7.83E-4||SGD DESC:Nonessential protein of unknown function; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; similar to mammalian BRP16 (Brain protein 16); relative distribution to the nucleus increases upon DNA replication stress Gene:IKI1(YHR187W)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin Gene:IKI3(YLR384C)|FD-Score:5.52|P-value:1.68E-8||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD) Gene:IOC3(YFR013W)|FD-Score:3.17|P-value:7.54E-4||SGD DESC:Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication Gene:ISU2(YOR226C)|FD-Score:3.1|P-value:9.69E-4||SGD DESC:Protein required for synthesis of iron-sulfur proteins; localized to the mitochondrial matrix; performs a scaffolding function in mitochondria during Fe/S cluster assembly; involved in Fe-S cluster assembly for both mitochondrial and cytosolic proteins; isu1 isu2 double mutant is inviable; protein abundance increases in response to DNA replication stress; evolutionarily conserved Gene:JEM1(YJL073W)|FD-Score:-4.5|P-value:3.43E-6||SGD DESC:DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 Gene:LSB6(YJL100W)|FD-Score:4.76|P-value:9.61E-7||SGD DESC:Type II phosphatidylinositol 4-kinase that binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization Gene:MAL13(YGR288W)|FD-Score:3.76|P-value:8.51E-5||SGD DESC:MAL-activator protein, part of complex locus MAL1; nonfunctional in genomic reference strain S288C Gene:MET10(YFR030W)|FD-Score:4.13|P-value:1.83E-5||SGD DESC:Subunit alpha of assimilatory sulfite reductase, which converts sulfite into sulfide Gene:MFA1(YDR461W)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA2 Gene:MFT1(YML062C)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance Gene:MLH2(YLR035C)|FD-Score:-3.87|P-value:5.38E-5||SGD DESC:Protein involved in the mismatch repair of certain frameshift intermediates and involved in meiotic recombination; forms a complex with Mlh1p Gene:MNE1(YOR350C)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Mitochondrial matrix protein involved in splicing Group I aI5-beta intron from COX1 mRNA Gene:MRC1(YCL061C)|FD-Score:4|P-value:3.16E-5||SGD DESC:S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres Gene:MRPL44(YMR225C)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MRPL51(YPR100W)|FD-Score:3.16|P-value:7.90E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NNT1(YLR285W)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:S-adenosylmethionine-dependent methyltransferase; has a role in rDNA silencing and in lifespan determination Gene:NOP12(YOL041C)|FD-Score:-3.32|P-value:4.52E-4||SGD DESC:Nucleolar protein involved in pre-25S rRNA processing and biogenesis of large 60S ribosomal subunit; contains an RNA recognition motif (RRM); binds to Ebp2; similar to Nop13p and Nsr1p Gene:OCA5(YHL029C_p)|FD-Score:3.47|P-value:2.65E-4||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:OSW1(YOR255W)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Protein involved in sporulation; required for the construction of the outer spore wall layers; required for proper localization of Spo14p Gene:PEX14(YGL153W)|FD-Score:3.26|P-value:5.60E-4||SGD DESC:Peroxisomal membrane peroxin that is a central component of the peroxisomal protein import machinery; interacts with both PTS1 (Pex5p) and PTS2 (Pex7p), peroxisomal matrix protein signal recognition factors and membrane receptor Pex13p Gene:PKH2(YOL100W)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p Gene:PUT4(YOR348C)|FD-Score:3.11|P-value:9.49E-4||SGD DESC:Proline permease, required for high-affinity transport of proline; also transports the toxic proline analog azetidine-2-carboxylate (AzC); PUT4 transcription is repressed in ammonia-grown cells Gene:RIC1(YLR039C)|FD-Score:3.99|P-value:3.30E-5||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPB9(YGL070C)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:RNA polymerase II subunit B12.6; contacts DNA; mutations affect transcription start site selection and fidelity of transcription Gene:RPP2A(YOL039W)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm Gene:RPS23B(YPR132W)|FD-Score:3.41|P-value:3.19E-4||SGD DESC:Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23B has a paralog, RPS23A, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal Gene:RRI2(YOL117W)|FD-Score:4.52|P-value:3.07E-6||SGD DESC:Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response Gene:SAP4(YGL229C)|FD-Score:-3.29|P-value:5.10E-4||SGD DESC:Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p Gene:SFB2(YNL049C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Component of the Sec23p-Sfb2p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb3p Gene:SKI3(YPR189W)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Ski complex component and TPR protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SNX4(YJL036W)|FD-Score:3.1|P-value:9.73E-4||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SPT3(YDR392W)|FD-Score:5.79|P-value:3.62E-9||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:TAT2(YOL020W)|FD-Score:3.09|P-value:9.97E-4||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:TMT1(YER175C)|FD-Score:3.22|P-value:6.36E-4||SGD DESC:Trans-aconitate methyltransferase, cytosolic enzyme that catalyzes the methyl esterification of 3-isopropylmalate, an intermediate of the leucine biosynthetic pathway, and trans-aconitate, which inhibits the citric acid cycle Gene:TRP4(YDR354W)|FD-Score:3.23|P-value:6.21E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:4.16|P-value:1.57E-5||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBP2(YOR124C)|FD-Score:4.2|P-value:1.35E-5||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; interacts with Rsp5p and is required for MVB sorting of membrane proteins; can cleave polyubiquitin and has isopeptidase activity Gene:VBA5(YKR105C_p)|FD-Score:-3.47|P-value:2.57E-4||SGD DESC:Plasma membrane protein of the Major Facilitator Superfamily (MFS); involved in amino acid uptake and drug sensitivity; similar to Vba3p Gene:VPS3(YDR495C)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS45(YGL095C)|FD-Score:3.77|P-value:8.11E-5||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YAL018C(YAL018C_p)|FD-Score:-3.72|P-value:9.90E-5||SGD DESC:Putative protein of unknown function Gene:YBR099C(YBR099C_d)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4 Gene:YCL002C(YCL002C_p)|FD-Score:3.12|P-value:9.18E-4||SGD DESC:Putative protein of unknown function; YCL002C is not an essential gene Gene:YDL144C(YDL144C_p)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL144C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YDR154C(YDR154C_d)|FD-Score:-3.31|P-value:4.72E-4||SGD DESC:Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein Gene:YER130C(YER130C_p)|FD-Score:4.59|P-value:2.25E-6||SGD DESC:Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress Gene:YHR180W(YHR180W_d)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL168W(YIL168W)|FD-Score:4.82|P-value:7.02E-7||SGD DESC:Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other <i>S. cerevisiae</i> strain backgrounds YIL168W and adjacent ORF, YIL167W, constitute a single ORF encoding L-serine dehydratase Gene:YMR075C-A(YMR075C-A_d)|FD-Score:-3.23|P-value:6.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RCO1/YMR075W Gene:YNL217W(YNL217W_p)|FD-Score:3.86|P-value:5.58E-5||SGD DESC:Putative protein of unknown function; weak sequence similarity to bis (5'-nucleotidyl)-tetraphosphatases; (GFP)-fusion protein localizes to the vacuole; null mutant is highly sensitive to azaserine and resistant to sodium-O-vandate Gene:YOL153C(YOL153C)|FD-Score:-5.5|P-value:1.93E-8||SGD DESC:Hypothetical protein Gene:YOR052C(YOR052C)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Nuclear protein of unknown function; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner and by weak acid; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress Gene:YPR039W(YPR039W_d)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified non essential genes ERV2/YPR037C and TIP41/YPR040W Gene:YPR076W(YPR076W_d)|FD-Score:4.18|P-value:1.48E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR097W(YPR097W)|FD-Score:3.21|P-value:6.56E-4||SGD DESC:Protein that contains a Phox homology (PX) domain and binds phosphoinositides; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:ZIP2(YGL249W)|FD-Score:4|P-value:3.23E-5||SGD DESC:Meiosis-specific protein; involved in normal synaptonemal complex formation and pairing between homologous chromosomes during meiosis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:BGL2(YGR282C)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Endo-beta-1,3-glucanase, major protein of the cell wall, involved in cell wall maintenance Gene:CAJ1(YER048C)|FD-Score:4.12|P-value:1.93E-5||SGD DESC:Nuclear type II J heat shock protein of the E. coli dnaJ family, contains a leucine zipper-like motif, binds to non-native substrates for presentation to Ssa3p, may function during protein translocation, assembly and disassembly Gene:CBC2(YPL178W)|FD-Score:-3.18|P-value:7.27E-4||SGD DESC:Small subunit of the heterodimeric cap binding complex that also contains Sto1p, component of the spliceosomal commitment complex; interacts with Npl3p, possibly to package mRNA for export from the nucleus; contains an RNA-binding motif Gene:CCW12(YLR110C)|FD-Score:3.93|P-value:4.34E-5||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CLC1(YGR167W)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:COG6(YNL041C)|FD-Score:5.36|P-value:4.10E-8||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DAP1(YPL170W)|FD-Score:3.14|P-value:8.42E-4||SGD DESC:Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis Gene:DOC1(YGL240W)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:Processivity factor required for the ubiquitination activity of the anaphase promoting complex (APC), mediates the activity of the APC by contributing to substrate recognition; involved in cyclin proteolysis; contains a conserved DOC1 homology domain Gene:ERV29(YGR284C)|FD-Score:4.7|P-value:1.29E-6||SGD DESC:Protein localized to COPII-coated vesicles; involved in vesicle formation and incorporation of specific secretory cargo; protein abundance increases in response to DNA replication stress Gene:FRE3(YOR381W)|FD-Score:3.3|P-value:4.86E-4||SGD DESC:Ferric reductase, reduces siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels Gene:FRE5(YOR384W)|FD-Score:3.85|P-value:5.95E-5||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FRK1(YPL141C)|FD-Score:4.26|P-value:1.00E-5||SGD DESC:Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; interacts with rRNA transcription and ribosome biogenesis factors and the long chain fatty acyl-CoA synthetase Faa3p; FRK1 has a paralog, KIN4, that arose from the whole genome duplication Gene:FUS2(YMR232W)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Cytoplasmic protein localized to the shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating Gene:GIR2(YDR152W)|FD-Score:3.74|P-value:9.12E-5||SGD DESC:Highly-acidic cytoplasmic RWD domain-containing protein of unknown function; forms a complex with Rbg2p; interacts with Rbg1p and Gcn1p; associates with translating ribosomes; putative intrinsically unstructured protein Gene:GLO1(YML004C)|FD-Score:4.02|P-value:2.92E-5||SGD DESC:Monomeric glyoxalase I, catalyzes the detoxification of methylglyoxal (a by-product of glycolysis) via condensation with glutathione to produce S-D-lactoylglutathione; expression regulated by methylglyoxal levels and osmotic stress Gene:GTO3(YMR251W)|FD-Score:3.85|P-value:5.87E-5||SGD DESC:Omega class glutathione transferase; putative cytosolic localization Gene:HGH1(YGR187C)|FD-Score:3.16|P-value:7.83E-4||SGD DESC:Nonessential protein of unknown function; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; similar to mammalian BRP16 (Brain protein 16); relative distribution to the nucleus increases upon DNA replication stress Gene:IKI1(YHR187W)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin Gene:IKI3(YLR384C)|FD-Score:5.52|P-value:1.68E-8||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD) Gene:IOC3(YFR013W)|FD-Score:3.17|P-value:7.54E-4||SGD DESC:Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication Gene:ISU2(YOR226C)|FD-Score:3.1|P-value:9.69E-4||SGD DESC:Protein required for synthesis of iron-sulfur proteins; localized to the mitochondrial matrix; performs a scaffolding function in mitochondria during Fe/S cluster assembly; involved in Fe-S cluster assembly for both mitochondrial and cytosolic proteins; isu1 isu2 double mutant is inviable; protein abundance increases in response to DNA replication stress; evolutionarily conserved Gene:JEM1(YJL073W)|FD-Score:-4.5|P-value:3.43E-6||SGD DESC:DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 Gene:LSB6(YJL100W)|FD-Score:4.76|P-value:9.61E-7||SGD DESC:Type II phosphatidylinositol 4-kinase that binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization Gene:MAL13(YGR288W)|FD-Score:3.76|P-value:8.51E-5||SGD DESC:MAL-activator protein, part of complex locus MAL1; nonfunctional in genomic reference strain S288C Gene:MET10(YFR030W)|FD-Score:4.13|P-value:1.83E-5||SGD DESC:Subunit alpha of assimilatory sulfite reductase, which converts sulfite into sulfide Gene:MFA1(YDR461W)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA2 Gene:MFT1(YML062C)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance Gene:MLH2(YLR035C)|FD-Score:-3.87|P-value:5.38E-5||SGD DESC:Protein involved in the mismatch repair of certain frameshift intermediates and involved in meiotic recombination; forms a complex with Mlh1p Gene:MNE1(YOR350C)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Mitochondrial matrix protein involved in splicing Group I aI5-beta intron from COX1 mRNA Gene:MRC1(YCL061C)|FD-Score:4|P-value:3.16E-5||SGD DESC:S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres Gene:MRPL44(YMR225C)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MRPL51(YPR100W)|FD-Score:3.16|P-value:7.90E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NNT1(YLR285W)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:S-adenosylmethionine-dependent methyltransferase; has a role in rDNA silencing and in lifespan determination Gene:NOP12(YOL041C)|FD-Score:-3.32|P-value:4.52E-4||SGD DESC:Nucleolar protein involved in pre-25S rRNA processing and biogenesis of large 60S ribosomal subunit; contains an RNA recognition motif (RRM); binds to Ebp2; similar to Nop13p and Nsr1p Gene:OCA5(YHL029C_p)|FD-Score:3.47|P-value:2.65E-4||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:OSW1(YOR255W)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Protein involved in sporulation; required for the construction of the outer spore wall layers; required for proper localization of Spo14p Gene:PEX14(YGL153W)|FD-Score:3.26|P-value:5.60E-4||SGD DESC:Peroxisomal membrane peroxin that is a central component of the peroxisomal protein import machinery; interacts with both PTS1 (Pex5p) and PTS2 (Pex7p), peroxisomal matrix protein signal recognition factors and membrane receptor Pex13p Gene:PKH2(YOL100W)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p Gene:PUT4(YOR348C)|FD-Score:3.11|P-value:9.49E-4||SGD DESC:Proline permease, required for high-affinity transport of proline; also transports the toxic proline analog azetidine-2-carboxylate (AzC); PUT4 transcription is repressed in ammonia-grown cells Gene:RIC1(YLR039C)|FD-Score:3.99|P-value:3.30E-5||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPB9(YGL070C)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:RNA polymerase II subunit B12.6; contacts DNA; mutations affect transcription start site selection and fidelity of transcription Gene:RPP2A(YOL039W)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm Gene:RPS23B(YPR132W)|FD-Score:3.41|P-value:3.19E-4||SGD DESC:Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23B has a paralog, RPS23A, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal Gene:RRI2(YOL117W)|FD-Score:4.52|P-value:3.07E-6||SGD DESC:Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response Gene:SAP4(YGL229C)|FD-Score:-3.29|P-value:5.10E-4||SGD DESC:Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p Gene:SFB2(YNL049C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Component of the Sec23p-Sfb2p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb3p Gene:SKI3(YPR189W)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Ski complex component and TPR protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SNX4(YJL036W)|FD-Score:3.1|P-value:9.73E-4||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SPT3(YDR392W)|FD-Score:5.79|P-value:3.62E-9||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:TAT2(YOL020W)|FD-Score:3.09|P-value:9.97E-4||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:TMT1(YER175C)|FD-Score:3.22|P-value:6.36E-4||SGD DESC:Trans-aconitate methyltransferase, cytosolic enzyme that catalyzes the methyl esterification of 3-isopropylmalate, an intermediate of the leucine biosynthetic pathway, and trans-aconitate, which inhibits the citric acid cycle Gene:TRP4(YDR354W)|FD-Score:3.23|P-value:6.21E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSC3(YBR058C-A)|FD-Score:4.16|P-value:1.57E-5||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBP2(YOR124C)|FD-Score:4.2|P-value:1.35E-5||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; interacts with Rsp5p and is required for MVB sorting of membrane proteins; can cleave polyubiquitin and has isopeptidase activity Gene:VBA5(YKR105C_p)|FD-Score:-3.47|P-value:2.57E-4||SGD DESC:Plasma membrane protein of the Major Facilitator Superfamily (MFS); involved in amino acid uptake and drug sensitivity; similar to Vba3p Gene:VPS3(YDR495C)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS45(YGL095C)|FD-Score:3.77|P-value:8.11E-5||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YAL018C(YAL018C_p)|FD-Score:-3.72|P-value:9.90E-5||SGD DESC:Putative protein of unknown function Gene:YBR099C(YBR099C_d)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4 Gene:YCL002C(YCL002C_p)|FD-Score:3.12|P-value:9.18E-4||SGD DESC:Putative protein of unknown function; YCL002C is not an essential gene Gene:YDL144C(YDL144C_p)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL144C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YDR154C(YDR154C_d)|FD-Score:-3.31|P-value:4.72E-4||SGD DESC:Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein Gene:YER130C(YER130C_p)|FD-Score:4.59|P-value:2.25E-6||SGD DESC:Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress Gene:YHR180W(YHR180W_d)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL168W(YIL168W)|FD-Score:4.82|P-value:7.02E-7||SGD DESC:Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other <i>S. cerevisiae</i> strain backgrounds YIL168W and adjacent ORF, YIL167W, constitute a single ORF encoding L-serine dehydratase Gene:YMR075C-A(YMR075C-A_d)|FD-Score:-3.23|P-value:6.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RCO1/YMR075W Gene:YNL217W(YNL217W_p)|FD-Score:3.86|P-value:5.58E-5||SGD DESC:Putative protein of unknown function; weak sequence similarity to bis (5'-nucleotidyl)-tetraphosphatases; (GFP)-fusion protein localizes to the vacuole; null mutant is highly sensitive to azaserine and resistant to sodium-O-vandate Gene:YOL153C(YOL153C)|FD-Score:-5.5|P-value:1.93E-8||SGD DESC:Hypothetical protein Gene:YOR052C(YOR052C)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Nuclear protein of unknown function; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner and by weak acid; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress Gene:YPR039W(YPR039W_d)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified non essential genes ERV2/YPR037C and TIP41/YPR040W Gene:YPR076W(YPR076W_d)|FD-Score:4.18|P-value:1.48E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR097W(YPR097W)|FD-Score:3.21|P-value:6.56E-4||SGD DESC:Protein that contains a Phox homology (PX) domain and binds phosphoinositides; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:ZIP2(YGL249W)|FD-Score:4|P-value:3.23E-5||SGD DESC:Meiosis-specific protein; involved in normal synaptonemal complex formation and pairing between homologous chromosomes during meiosis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR062C18.601.63E-7713.40RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YPR178W5.238.47E-80.64PRP4Splicing factor, component of the U4/U6-U5 snRNP complex
YDL141W4.592.19E-60.14BPL1Biotin:apoprotein ligase, covalently modifies proteins with the addition of biotin, required for acetyl-CoA carboxylase (Acc1p) holoenzyme formation
YOR204W4.454.35E-60.23DED1ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility
YNL126W4.211.27E-50.10SPC98Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
YNL221C4.111.94E-50.53POP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
YKR083C3.591.67E-40.28DAD2Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
YNL216W3.314.62E-40.06RAP1Essential DNA-binding transcription regulator that binds at many loci; involved in either transcription activation or repression, chromatin silencing, and telomere length maintenance; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain)
YJR017C3.255.76E-40.10ESS1Peptidylprolyl-cis/trans-isomerase (PPIase); specific for phosphorylated serine and threonine residues N-terminal to proline; regulates phosphorylation of the RNAP II large subunit (Rpo21p) C-terminal domain (CTD); associates with phospho-Ser5 form of RNAP II in vivo; regulates phosphorylation of Ser7 within CTD; present along entire coding length of genes; represses initiation of CUTs; required for efficient termination of mRNA transcription and trimethylation of histone H3
YFR003C3.168.02E-40.13YPI1Regulatory subunit of the type I protein phosphatase (PP1) Glc7p; Glc7p participates in the regulation of a variety of metabolic processes including mitosis and glycogen metabolism; in vitro evidence suggests Ypi1p is an inhibitor of Glc7p while in vivo evidence suggests it is an activator; overproduction causes decreased cellular content of glycogen; partial depletion causes lithium sensitivity, while overproduction confers lithium-tolerance
YNL178W3.020.001240.03RPS3Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3
YGR114C_d2.990.001390.17YGR114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
YMR001C2.820.002370.16CDC5Polo-like kinase with multiple functions in mitosis and cytokinesis through substrate phosphorylation, also functions in adaptation to DNA damage during meiosis; has similarity to Xenopus Plx1 and S. pombe Plo1p; possible Cdc28p substrate
YFR050C2.660.003850.00PRE4Beta 7 subunit of the 20S proteasome
YDL193W2.660.003900.01NUS1Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR392W5.793.62E-9SPT3Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters
YLR384C5.521.68E-8IKI3Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD)
YNL041C5.364.10E-8COG6Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YIL168W4.827.02E-7YIL168WOpen reading frame, unlikely to produce a functional protein in S288C; in closely related species and other S. cerevisiae strain backgrounds YIL168W and adjacent ORF, YIL167W, constitute a single ORF encoding L-serine dehydratase
YJL100W4.769.61E-7LSB6Type II phosphatidylinositol 4-kinase that binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization
YGR284C4.701.29E-6ERV29Protein localized to COPII-coated vesicles; involved in vesicle formation and incorporation of specific secretory cargo; protein abundance increases in response to DNA replication stress
YER130C_p4.592.25E-6YER130C_pProtein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress
YOL117W4.523.07E-6RRI2Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response
YPL141C4.261.00E-5FRK1Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; interacts with rRNA transcription and ribosome biogenesis factors and the long chain fatty acyl-CoA synthetase Faa3p; FRK1 has a paralog, KIN4, that arose from the whole genome duplication
YOR124C4.201.35E-5UBP2Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; interacts with Rsp5p and is required for MVB sorting of membrane proteins; can cleave polyubiquitin and has isopeptidase activity
YPR076W_d4.181.48E-5YPR076W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YBR058C-A4.161.57E-5TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YFR030W4.131.83E-5MET10Subunit alpha of assimilatory sulfite reductase, which converts sulfite into sulfide
YER048C4.121.93E-5CAJ1Nuclear type II J heat shock protein of the E. coli dnaJ family, contains a leucine zipper-like motif, binds to non-native substrates for presentation to Ssa3p, may function during protein translocation, assembly and disassembly
YML004C4.022.92E-5GLO1Monomeric glyoxalase I, catalyzes the detoxification of methylglyoxal (a by-product of glycolysis) via condensation with glutathione to produce S-D-lactoylglutathione; expression regulated by methylglyoxal levels and osmotic stress

GO enrichment analysis for SGTC_265
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2464.00E-82SGTC_2685mycophenolic acid 18.9 μMMiscellaneous4465411RNA pol III & RNase P/MRP
0.2281.44E-70SGTC_32619137475 49.5 μMChembridge (Drug-like library)170276900.0689655RPP1 & pyrimidine depletion
0.2181.39E-64SGTC_1661st013063 9.7 μMTimTec (Natural product derivative library)7213950.16
0.2091.64E-59SGTC_15346-azauridine 81.6 μMTimTec (Pure natural product library)2335020.0769231RNA pol III & RNase P/MRP
0.2093.47E-59SGTC_1662st014054 52.0 μMTimTec (Natural product derivative library)28387770.0879121TSC3-RPN4
0.1984.79E-53SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.0657895TSC3-RPN4
0.1943.46E-51SGTC_24984'-Methoxychalcone 54.6 μMMicrosource (Natural product library)6418180.130435RPP1 & pyrimidine depletion
0.1764.14E-42SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.025974TSC3-RPN4
0.1742.32E-41SGTC_2350330-0087 5.0 mMChemDiv (Drug-like library)464948180.0731707TSC3-RPN4
0.1702.22E-39SGTC_10164226-1401 38.0 μMChemDiv (Drug-like library)29016130.0879121RNA pol III & RNase P/MRP
0.1702.18E-39SGTC_2686alizarin 95.4 μMMiscellaneous62930.107692RPP1 & pyrimidine depletion
0.1672.73E-38SGTC_6790422-0091 41.4 μMChemDiv (Drug-like library)6273840.0136986Golgi
0.1666.28E-38SGTC_23236148992 148.3 μMChembridge (Fragment library)28882200.105263
0.1661.40E-37SGTC_1872amphotericin b 148.0 nMMicrosource (Natural product library)166676790.0940171RNA pol III & RNase P/MRP
0.1652.09E-37SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.119048

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2685mycophenolic acid18.85 μM1446541Miscellaneous320.33713.15726RNA pol III & RNase P/MRP
SGTC_920mycophenolic acid780.42 nM1446541TimTec (Natural product library)320.33713.15726
SGTC_1123mangostin811.29 nM0.3382355281650TimTec (Natural product library)410.459645.93536mitochondrial response to ROS
SGTC_2594a-mangostin100 μM0.27142945934201Microsource (Natural product library)452.539386.61106
SGTC_2496diffractaic acid79.83 μM0.22857194870Microsource (Natural product library)374.384484.56127
SGTC_918glabranin770.73 nM0.220779124049TimTec (Natural product library)324.37044.47224
SGTC_2529gangaleoidin93.61 μM0.2179494565183Microsource (Natural product library)413.205564.8917
SGTC_2589lobaric acid100 μM0.20689773157Microsource (Natural product library)456.485025.99328
SGTC_2561acetosyringone100 μM0.2033917198Microsource (Natural product library)196.199881.29514
SGTC_1959st07709155.8 μM0.197183821356TimTec (Natural product derivative library)314.289462.61926RPP1 & pyrimidine depletion
SGTC_1593justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one54.9 μM0.194805442882TimTec (Pure natural product library)364.348143.60106redox potentiating