dihydrofissinolide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2653
Screen concentration 100.0 μM
Source Microsource (Natural product library)
PubChem CID 6857746
SMILES CC(=O)OC1C2CC3C(CCC4(C3CC(=O)OC4C5=COC=C5)C)C(C2=O)(C(C1(C)C)CC(=O)OC)C
Standardized SMILES COC(=O)CC1C(C)(C)C(OC(=O)C)C2CC3C(CCC4(C)C3CC(=O)OC4c5cocc5)C1(C)C2=O
Molecular weight 514.6072
ALogP 3.41
H-bond donor count 0
H-bond acceptor count 8
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.8
% growth inhibition (Hom. pool) 2.64


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6857746
Download HIP data (tab-delimited text)  (excel)
Gene:ARP9(YMR033W)|FD-Score:5.68|P-value:6.69E-9|Clearance:1.93||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:DPS1(YLL018C)|FD-Score:-3.39|P-value:3.46E-4|Clearance:0||SGD DESC:Aspartyl-tRNA synthetase, primarily cytoplasmic; homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation Gene:IMP3(YHR148W)|FD-Score:3.37|P-value:3.80E-4|Clearance:0.03||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA Gene:PBN1(YCL052C)|FD-Score:3.2|P-value:6.80E-4|Clearance:0.02||SGD DESC:Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X Gene:PRE1(YER012W)|FD-Score:-4.25|P-value:1.09E-5|Clearance:0||SGD DESC:Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle Gene:RLP7(YNL002C)|FD-Score:3.75|P-value:8.69E-5|Clearance:0.29||SGD DESC:Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs Gene:RPB10(YOR210W)|FD-Score:3.19|P-value:7.21E-4|Clearance:0.21||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:SEC23(YPR181C)|FD-Score:3.47|P-value:2.62E-4|Clearance:0.1||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:SHR3(YDL212W)|FD-Score:3.33|P-value:4.30E-4|Clearance:0.06||SGD DESC:Endoplasmic reticulum packaging chaperone, required for incorporation of amino acid permeases into COPII coated vesicles for transport to the cell surface Gene:URA6(YKL024C)|FD-Score:3.28|P-value:5.26E-4|Clearance:0.07||SGD DESC:Uridylate kinase, catalyzes the seventh enzymatic step in the de novo biosynthesis of pyrimidines, converting uridine monophosphate (UMP) into uridine-5'-diphosphate (UDP) Gene:ARP9(YMR033W)|FD-Score:5.68|P-value:6.69E-9|Clearance:1.93||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:DPS1(YLL018C)|FD-Score:-3.39|P-value:3.46E-4|Clearance:0||SGD DESC:Aspartyl-tRNA synthetase, primarily cytoplasmic; homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation Gene:IMP3(YHR148W)|FD-Score:3.37|P-value:3.80E-4|Clearance:0.03||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA Gene:PBN1(YCL052C)|FD-Score:3.2|P-value:6.80E-4|Clearance:0.02||SGD DESC:Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X Gene:PRE1(YER012W)|FD-Score:-4.25|P-value:1.09E-5|Clearance:0||SGD DESC:Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle Gene:RLP7(YNL002C)|FD-Score:3.75|P-value:8.69E-5|Clearance:0.29||SGD DESC:Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs Gene:RPB10(YOR210W)|FD-Score:3.19|P-value:7.21E-4|Clearance:0.21||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:SEC23(YPR181C)|FD-Score:3.47|P-value:2.62E-4|Clearance:0.1||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:SHR3(YDL212W)|FD-Score:3.33|P-value:4.30E-4|Clearance:0.06||SGD DESC:Endoplasmic reticulum packaging chaperone, required for incorporation of amino acid permeases into COPII coated vesicles for transport to the cell surface Gene:URA6(YKL024C)|FD-Score:3.28|P-value:5.26E-4|Clearance:0.07||SGD DESC:Uridylate kinase, catalyzes the seventh enzymatic step in the de novo biosynthesis of pyrimidines, converting uridine monophosphate (UMP) into uridine-5'-diphosphate (UDP)

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6857746
Download HOP data (tab-delimited text)  (excel)
Gene:ACM1(YPL267W)|FD-Score:-3.4|P-value:3.38E-4||SGD DESC:Pseudosubstrate inhibitor of the anaphase-promoting complex/cyclosome (APC/C), that suppresses APC/C [Cdh1]-mediated proteolysis of mitotic cyclins; associates with Cdh1p, Bmh1p and Bmh2p; cell cycle regulated protein Gene:CCH1(YGR217W)|FD-Score:3.16|P-value:7.96E-4||SGD DESC:Voltage-gated high-affinity calcium channel involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and co-localizes with Mid1p, suggesting Cch1p and Mid1p function together Gene:CHA1(YCL064C)|FD-Score:3.29|P-value:5.04E-4||SGD DESC:Catabolic L-serine (L-threonine) deaminase, catalyzes the degradation of both L-serine and L-threonine; required to use serine or threonine as the sole nitrogen source, transcriptionally induced by serine and threonine Gene:CST6(YIL036W)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor, in ATF/CREB family; mediates transcriptional activation of NCE103 (encoding carbonic anhydrase) in response to low CO2 levels such as in the ambient air; proposed to be a regulator of oleate responsive genes; involved in utilization of non-optimal carbon sources and chromosome stability; CST6 has a paralog, ACA1, that arose from the whole genome duplication Gene:DNF1(YER166W)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase Gene:FLO1(YAR050W)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Lectin-like protein involved in flocculation, cell wall protein that binds to mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin sensitive and heat resistant; similar to Flo5p Gene:GSH2(YOL049W)|FD-Score:-4.17|P-value:1.50E-5||SGD DESC:Glutathione synthetase, catalyzes the ATP-dependent synthesis of glutathione (GSH) from gamma-glutamylcysteine and glycine; induced by oxidative stress and heat shock Gene:LRG1(YDL240W)|FD-Score:-3.24|P-value:5.90E-4||SGD DESC:Putative GTPase-activating protein (GAP) involved in the Pkc1p-mediated signaling pathway that controls cell wall integrity; appears to specifically regulate 1,3-beta-glucan synthesis Gene:MTC3(YGL226W)|FD-Score:3.3|P-value:4.87E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1 Gene:MZM1(YDR493W)|FD-Score:3.26|P-value:5.65E-4||SGD DESC:Protein required for assembly of the cytochrome bc(1) complex; acts as a chaperone for Rip1p and facilitates its insertion into the complex at a late stage of assembly; localized to the mitochondrial matrix; null mutant exhibits a respiratory growth defect and reduced mitochondrial zinc levels, which is characteristic of mutations affecting bc(1) complex assembly; human LYRM7 is a functional ortholog Gene:NHA1(YLR138W)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Na+/H+ antiporter involved in sodium and potassium efflux through the plasma membrane; required for alkali cation tolerance at acidic pH Gene:NMA111(YNL123W)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:OAZ1(YPL052W)|FD-Score:3.17|P-value:7.51E-4||SGD DESC:Regulator of ornithine decarboxylase (Spe1p), antizyme that binds to Spe1p to stimulate ubiquitin-independent degradation by the proteasome; binding of polyamines to nascent Oaz1p during translation stimulates +1 ribosomal frameshifting, allowing translation of full-length Oaz1p Gene:PDR8(YLR266C)|FD-Score:3.14|P-value:8.31E-4||SGD DESC:Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; PDR8 has a paralog, YRR1, that arose from the whole genome duplication Gene:PIH1(YHR034C)|FD-Score:4|P-value:3.19E-5||SGD DESC:Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II Gene:PSP2(YML017W)|FD-Score:-3.44|P-value:2.87E-4||SGD DESC:Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing Gene:SCH9(YHR205W)|FD-Score:3.74|P-value:9.06E-5||SGD DESC:AGC family protein kinase; functional ortholog of mammalian S6 kinase; phosphorylated by Tor1p and required for TORC1-mediated regulation of ribosome biogenesis, translation initiation, and entry into G0 phase; involved in transactivation of osmostress-responsive genes; regulates G1 progression, cAPK activity and nitrogen activation of the FGM pathway; integrates nutrient signals and stress signals from sphingolipids to regulate lifespan Gene:SSM4(YIL030C)|FD-Score:3.26|P-value:5.62E-4||SGD DESC:Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation Gene:YBR284W(YBR284W_p)|FD-Score:6.03|P-value:8.44E-10||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication Gene:YDL073W(YDL073W_p)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Putative protein of unknown function; YDL073W is not an essential gene Gene:YER130C(YER130C_p)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress Gene:YGL176C(YGL176C_p)|FD-Score:4.2|P-value:1.31E-5||SGD DESC:Putative protein of unknown function; deletion mutant is viable and has no detectable phenotype Gene:YIL163C(YIL163C_p)|FD-Score:3.15|P-value:8.08E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YMR294W-A(YMR294W-A_d)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, substantially overlaps YMR295C; deletion causes sensitivity to unfolded protein response-inducing agents Gene:YNL228W(YNL228W_d)|FD-Score:3.17|P-value:7.66E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF YNL227C/JJJ1 Gene:YNR064C(YNR064C)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Epoxide hydrolase, member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides Gene:YOL118C(YOL118C_d)|FD-Score:3.22|P-value:6.48E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL034W(YPL034W_p)|FD-Score:-3.12|P-value:8.98E-4||SGD DESC:Putative protein of unknown function; YPL034W is not essential gene Gene:YPL247C(YPL247C_p)|FD-Score:3.29|P-value:5.00E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest Gene:YPR077C(YPR077C_d)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression increased by deletion of NAP1 Gene:ACM1(YPL267W)|FD-Score:-3.4|P-value:3.38E-4||SGD DESC:Pseudosubstrate inhibitor of the anaphase-promoting complex/cyclosome (APC/C), that suppresses APC/C [Cdh1]-mediated proteolysis of mitotic cyclins; associates with Cdh1p, Bmh1p and Bmh2p; cell cycle regulated protein Gene:CCH1(YGR217W)|FD-Score:3.16|P-value:7.96E-4||SGD DESC:Voltage-gated high-affinity calcium channel involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and co-localizes with Mid1p, suggesting Cch1p and Mid1p function together Gene:CHA1(YCL064C)|FD-Score:3.29|P-value:5.04E-4||SGD DESC:Catabolic L-serine (L-threonine) deaminase, catalyzes the degradation of both L-serine and L-threonine; required to use serine or threonine as the sole nitrogen source, transcriptionally induced by serine and threonine Gene:CST6(YIL036W)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor, in ATF/CREB family; mediates transcriptional activation of NCE103 (encoding carbonic anhydrase) in response to low CO2 levels such as in the ambient air; proposed to be a regulator of oleate responsive genes; involved in utilization of non-optimal carbon sources and chromosome stability; CST6 has a paralog, ACA1, that arose from the whole genome duplication Gene:DNF1(YER166W)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase Gene:FLO1(YAR050W)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Lectin-like protein involved in flocculation, cell wall protein that binds to mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin sensitive and heat resistant; similar to Flo5p Gene:GSH2(YOL049W)|FD-Score:-4.17|P-value:1.50E-5||SGD DESC:Glutathione synthetase, catalyzes the ATP-dependent synthesis of glutathione (GSH) from gamma-glutamylcysteine and glycine; induced by oxidative stress and heat shock Gene:LRG1(YDL240W)|FD-Score:-3.24|P-value:5.90E-4||SGD DESC:Putative GTPase-activating protein (GAP) involved in the Pkc1p-mediated signaling pathway that controls cell wall integrity; appears to specifically regulate 1,3-beta-glucan synthesis Gene:MTC3(YGL226W)|FD-Score:3.3|P-value:4.87E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1 Gene:MZM1(YDR493W)|FD-Score:3.26|P-value:5.65E-4||SGD DESC:Protein required for assembly of the cytochrome bc(1) complex; acts as a chaperone for Rip1p and facilitates its insertion into the complex at a late stage of assembly; localized to the mitochondrial matrix; null mutant exhibits a respiratory growth defect and reduced mitochondrial zinc levels, which is characteristic of mutations affecting bc(1) complex assembly; human LYRM7 is a functional ortholog Gene:NHA1(YLR138W)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Na+/H+ antiporter involved in sodium and potassium efflux through the plasma membrane; required for alkali cation tolerance at acidic pH Gene:NMA111(YNL123W)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:OAZ1(YPL052W)|FD-Score:3.17|P-value:7.51E-4||SGD DESC:Regulator of ornithine decarboxylase (Spe1p), antizyme that binds to Spe1p to stimulate ubiquitin-independent degradation by the proteasome; binding of polyamines to nascent Oaz1p during translation stimulates +1 ribosomal frameshifting, allowing translation of full-length Oaz1p Gene:PDR8(YLR266C)|FD-Score:3.14|P-value:8.31E-4||SGD DESC:Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; PDR8 has a paralog, YRR1, that arose from the whole genome duplication Gene:PIH1(YHR034C)|FD-Score:4|P-value:3.19E-5||SGD DESC:Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II Gene:PSP2(YML017W)|FD-Score:-3.44|P-value:2.87E-4||SGD DESC:Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing Gene:SCH9(YHR205W)|FD-Score:3.74|P-value:9.06E-5||SGD DESC:AGC family protein kinase; functional ortholog of mammalian S6 kinase; phosphorylated by Tor1p and required for TORC1-mediated regulation of ribosome biogenesis, translation initiation, and entry into G0 phase; involved in transactivation of osmostress-responsive genes; regulates G1 progression, cAPK activity and nitrogen activation of the FGM pathway; integrates nutrient signals and stress signals from sphingolipids to regulate lifespan Gene:SSM4(YIL030C)|FD-Score:3.26|P-value:5.62E-4||SGD DESC:Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation Gene:YBR284W(YBR284W_p)|FD-Score:6.03|P-value:8.44E-10||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication Gene:YDL073W(YDL073W_p)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Putative protein of unknown function; YDL073W is not an essential gene Gene:YER130C(YER130C_p)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress Gene:YGL176C(YGL176C_p)|FD-Score:4.2|P-value:1.31E-5||SGD DESC:Putative protein of unknown function; deletion mutant is viable and has no detectable phenotype Gene:YIL163C(YIL163C_p)|FD-Score:3.15|P-value:8.08E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YMR294W-A(YMR294W-A_d)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, substantially overlaps YMR295C; deletion causes sensitivity to unfolded protein response-inducing agents Gene:YNL228W(YNL228W_d)|FD-Score:3.17|P-value:7.66E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF YNL227C/JJJ1 Gene:YNR064C(YNR064C)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Epoxide hydrolase, member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides Gene:YOL118C(YOL118C_d)|FD-Score:3.22|P-value:6.48E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL034W(YPL034W_p)|FD-Score:-3.12|P-value:8.98E-4||SGD DESC:Putative protein of unknown function; YPL034W is not essential gene Gene:YPL247C(YPL247C_p)|FD-Score:3.29|P-value:5.00E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest Gene:YPR077C(YPR077C_d)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression increased by deletion of NAP1

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YMR033W5.686.69E-91.93ARP9Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YNL002C3.758.69E-50.29RLP7Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs
YPR181C3.472.62E-40.10SEC23GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p
YHR148W3.373.80E-40.03IMP3Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA
YDL212W3.334.30E-40.06SHR3Endoplasmic reticulum packaging chaperone, required for incorporation of amino acid permeases into COPII coated vesicles for transport to the cell surface
YKL024C3.285.26E-40.07URA6Uridylate kinase, catalyzes the seventh enzymatic step in the de novo biosynthesis of pyrimidines, converting uridine monophosphate (UMP) into uridine-5'-diphosphate (UDP)
YCL052C3.206.80E-40.02PBN1Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X
YOR210W3.197.21E-40.21RPB10RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III
YFL002C2.970.001470.04SPB4Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients
YPL093W2.930.001700.10NOG1Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins
YOR310C2.830.002360.00NOP58Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA
YGL065C2.820.002380.02ALG2Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol
YKL004W2.800.002520.07AUR1Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance
YER006W2.730.003140.01NUG1GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus
YBL020W2.730.003210.11RFT1Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR284W_p6.038.44E-10YBR284W_pPutative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication
YGL176C_p4.201.31E-5YGL176C_pPutative protein of unknown function; deletion mutant is viable and has no detectable phenotype
YHR034C4.003.19E-5PIH1Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II
YHR205W3.749.06E-5SCH9AGC family protein kinase; functional ortholog of mammalian S6 kinase; phosphorylated by Tor1p and required for TORC1-mediated regulation of ribosome biogenesis, translation initiation, and entry into G0 phase; involved in transactivation of osmostress-responsive genes; regulates G1 progression, cAPK activity and nitrogen activation of the FGM pathway; integrates nutrient signals and stress signals from sphingolipids to regulate lifespan
YER130C_p3.711.05E-4YER130C_pProtein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress
YNL123W3.651.33E-4NMA111Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases
YLR138W3.512.22E-4NHA1Na+/H+ antiporter involved in sodium and potassium efflux through the plasma membrane; required for alkali cation tolerance at acidic pH
YNR064C3.472.58E-4YNR064CEpoxide hydrolase, member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides
YDL073W_p3.442.90E-4YDL073W_pPutative protein of unknown function; YDL073W is not an essential gene
YAR050W3.413.25E-4FLO1Lectin-like protein involved in flocculation, cell wall protein that binds to mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin sensitive and heat resistant; similar to Flo5p
YMR294W-A_d3.353.99E-4YMR294W-A_dDubious open reading frame unlikely to encode a functional protein, substantially overlaps YMR295C; deletion causes sensitivity to unfolded protein response-inducing agents
YER166W3.314.70E-4DNF1Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase
YPR077C_d3.304.85E-4YPR077C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression increased by deletion of NAP1
YGL226W3.304.87E-4MTC3Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1
YPL247C_p3.295.00E-4YPL247C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest

GO enrichment analysis for SGTC_2653
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0944.75E-13SGTC_22297354935 190.0 μMChembridge (Fragment library)3181120.0526316RSC complex & mRNA processing
0.0781.64E-9SGTC_1702st027038 51.0 μMTimTec (Natural product derivative library)55211820.0495868
0.0759.88E-9SGTC_13873342-1166 1.8 μMChemDiv (Drug-like library)34182280.0736842mitochondrial processes
0.0741.01E-8SGTC_698k064-0052 127.0 μMChemDiv (Drug-like library)50887510.0555556DNA damage response
0.0691.00E-7SGTC_3730844-0104 25.9 μMChemDiv (Drug-like library)53758040.111111ERAD & cell cycle
0.0681.41E-7SGTC_29759084847 53.7 μMChembridge (Drug-like library)272117240.0693069
0.0655.07E-7SGTC_2709st078560 56.0 μMTimTec (Natural product derivative library)172509030.0789474
0.0647.78E-7SGTC_21575649910 96.4 μMChembridge (Fragment library)28580910.0425532
0.0621.93E-6SGTC_1162k048-0130 20.1 μMChemDiv (Drug-like library)67974060.03Golgi
0.0612.80E-6SGTC_1090efavirenz 20.7 μMMiscellaneous641390.0970874
0.0603.84E-6SGTC_9861493-0483 84.0 μMChemDiv (Drug-like library)400790.107527
0.0586.88E-6SGTC_23286201408 200.0 μMChembridge (Fragment library)28904180.104762
0.0587.67E-6SGTC_1609st001574 46.4 μMTimTec (Natural product derivative library)39600120.213592
0.0588.30E-6SGTC_15447-methoxy-4-methylcoumarin 105.0 μMTimTec (Pure natural product library)3908070.1
0.0571.17E-5SGTC_30279089541 49.5 μMChembridge (Drug-like library)99438190.0947368fatty acid desaturase (OLE1)

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_26373-alpha-acetoxydihydrodeoxygedunin100 μM0.3870976708514Microsource (Natural product library)512.63444.51907
SGTC_1586deoxycorticosterone acetate53.7 μM0.2268045952TimTec (Pure natural product library)372.497783.56704
SGTC_1587estradiol valerate56.1 μM0.21568613791TimTec (Pure natural product library)356.498385.79613
SGTC_416estradiol valerate100 μM0.21568613791TimTec (Pure natural product library)356.498385.79613
SGTC_1609st00157446.4 μM0.2135923960012TimTec (Natural product derivative library)430.576923.5615
SGTC_1575cyproterone acetate48 μM0.219880Miscellaneous416.937663.31404
SGTC_1517deoxycholic acid methyl ester49.2 μM0.207921229346TimTec (Pure natural product library)406.598584.30724
SGTC_2531methyl deoxycholate70.07 μM0.20792116667744Microsource (Natural product library)406.598584.30724
SGTC_387norethindrone acetate66.7 μM0.20202541197Miscellaneous340.455924.69403
SGTC_1877wortmannin25 μM0.201835312145ICCB bioactive library428.431861.70508
SGTC_2555cholic acid100 μM0.1941756708657Microsource (Natural product library)422.597983.13835