protoveratrine a

A heart drug and vasodilator.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2654
Screen concentration 100.0 μM
Source Microsource (Natural product library)
PubChem CID 5702278
SMILES CCC(C)C(=O)OC1C(C2C(CN3CC(CCC3C2(C)O)C)C4C1(C5C(C(C6C7(C5(C4)OC6(C(CC7)OC(=O)C(C)(CC)O)O)C)OC(=O)C)OC(=O)C)O)O
Standardized SMILES CCC(C)C(=O)OC1C(O)C2C(CN3CC(C)CCC3C2(C)O)C4CC56OC7(O)C(CCC5(C)C7C(OC(=O)C)C(OC(=O)C)C6C14O)OC(=O)C(C)(O)CC
Molecular weight 793.9372
ALogP 1.84
H-bond donor count 5
H-bond acceptor count 15
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 10.05
% growth inhibition (Hom. pool) 2.64


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5702278
Download HIP data (tab-delimited text)  (excel)
Gene:DHR2(YKL078W)|FD-Score:3.17|P-value:7.67E-4|Clearance:0.03||SGD DESC:Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis Gene:MAK5(YBR142W)|FD-Score:3.14|P-value:8.39E-4|Clearance:0.23||SGD DESC:Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits Gene:OST2(YOR103C)|FD-Score:3.49|P-value:2.43E-4|Clearance:0.15||SGD DESC:Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:RPL18A(YOL120C)|FD-Score:3.34|P-value:4.14E-4|Clearance:0.18||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RSC58(YLR033W)|FD-Score:5.05|P-value:2.25E-7|Clearance:0.58||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SSL1(YLR005W)|FD-Score:-3.26|P-value:5.65E-4|Clearance:0||SGD DESC:Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p Gene:SUI3(YPL237W)|FD-Score:3.85|P-value:5.95E-5|Clearance:0.07||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:TFA1(YKL028W)|FD-Score:3.85|P-value:5.81E-5|Clearance:0.01||SGD DESC:TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening Gene:TFA2(YKR062W)|FD-Score:3.78|P-value:7.85E-5|Clearance:0.21||SGD DESC:TFIIE small subunit, involved in RNA polymerase II transcription initiation Gene:YPI1(YFR003C)|FD-Score:3.57|P-value:1.80E-4|Clearance:0.08||SGD DESC:Regulatory subunit of the type I protein phosphatase (PP1) Glc7p; Glc7p participates in the regulation of a variety of metabolic processes including mitosis and glycogen metabolism; in vitro evidence suggests Ypi1p is an inhibitor of Glc7p while in vivo evidence suggests it is an activator; overproduction causes decreased cellular content of glycogen; partial depletion causes lithium sensitivity, while overproduction confers lithium-tolerance Gene:YPR136C(YPR136C_d)|FD-Score:4.07|P-value:2.40E-5|Clearance:0.21||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9 Gene:YTM1(YOR272W)|FD-Score:4.65|P-value:1.68E-6|Clearance:0.58||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required for maturation of the large ribosomal subunit; has seven C-terminal WD repeats Gene:DHR2(YKL078W)|FD-Score:3.17|P-value:7.67E-4|Clearance:0.03||SGD DESC:Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis Gene:MAK5(YBR142W)|FD-Score:3.14|P-value:8.39E-4|Clearance:0.23||SGD DESC:Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits Gene:OST2(YOR103C)|FD-Score:3.49|P-value:2.43E-4|Clearance:0.15||SGD DESC:Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:RPL18A(YOL120C)|FD-Score:3.34|P-value:4.14E-4|Clearance:0.18||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RSC58(YLR033W)|FD-Score:5.05|P-value:2.25E-7|Clearance:0.58||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SSL1(YLR005W)|FD-Score:-3.26|P-value:5.65E-4|Clearance:0||SGD DESC:Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p Gene:SUI3(YPL237W)|FD-Score:3.85|P-value:5.95E-5|Clearance:0.07||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:TFA1(YKL028W)|FD-Score:3.85|P-value:5.81E-5|Clearance:0.01||SGD DESC:TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening Gene:TFA2(YKR062W)|FD-Score:3.78|P-value:7.85E-5|Clearance:0.21||SGD DESC:TFIIE small subunit, involved in RNA polymerase II transcription initiation Gene:YPI1(YFR003C)|FD-Score:3.57|P-value:1.80E-4|Clearance:0.08||SGD DESC:Regulatory subunit of the type I protein phosphatase (PP1) Glc7p; Glc7p participates in the regulation of a variety of metabolic processes including mitosis and glycogen metabolism; in vitro evidence suggests Ypi1p is an inhibitor of Glc7p while in vivo evidence suggests it is an activator; overproduction causes decreased cellular content of glycogen; partial depletion causes lithium sensitivity, while overproduction confers lithium-tolerance Gene:YPR136C(YPR136C_d)|FD-Score:4.07|P-value:2.40E-5|Clearance:0.21||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9 Gene:YTM1(YOR272W)|FD-Score:4.65|P-value:1.68E-6|Clearance:0.58||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required for maturation of the large ribosomal subunit; has seven C-terminal WD repeats

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5702278
Download HOP data (tab-delimited text)  (excel)
Gene:ALE1(YOR175C)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Broad-specificity lysophospholipid acyltransferase, part of MBOAT family of membrane-bound O-acyltransferases; key component of Lands cycle; may have role in fatty acid exchange at sn-2 position of mature glycerophospholipids Gene:ATG21(YPL100W)|FD-Score:3.25|P-value:5.80E-4||SGD DESC:Phosphoinositide binding protein required for vesicle formation in the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein Gene:CAR1(YPL111W)|FD-Score:3.14|P-value:8.56E-4||SGD DESC:Arginase, responsible for arginine degradation, expression responds to both induction by arginine and nitrogen catabolite repression; disruption enhances freeze tolerance Gene:CIN1(YOR349W)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:COX9(YDL067C)|FD-Score:-3.15|P-value:8.28E-4||SGD DESC:Subunit VIIa of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:DAL3(YIR032C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Ureidoglycolate hydrolase, converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:DCC1(YCL016C)|FD-Score:3.8|P-value:7.22E-5||SGD DESC:Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance Gene:EST1(YLR233C)|FD-Score:-3.73|P-value:9.59E-5||SGD DESC:TLC1 RNA-associated factor involved in telomere length regulation as recruitment subunit of telomerase; has G-quadruplex promoting activity that is required for telomere elongation; possible role in activating telomere-bound Est2p-TLC1-RNA Gene:GLO2(YDR272W)|FD-Score:3.9|P-value:4.81E-5||SGD DESC:Cytoplasmic glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate Gene:GYP1(YOR070C)|FD-Score:-3.4|P-value:3.40E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HRK1(YOR267C)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Protein kinase; implicated in activation of the plasma membrane H(+)-ATPase Pma1p in response to glucose metabolism; plays a role in ion homeostasis; protein abundance increases in response to DNA replication stress Gene:IRC13(YOR235W_d)|FD-Score:3.31|P-value:4.65E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci Gene:IRC18(YJL037W)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Putative protein of unknown function; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRC7(YFR055W_p)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:Beta-lyase involved in the production of thiols; null mutant displays increased levels of spontaneous Rad52p foci; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner and by copper levels in a Mac1-dependent manner Gene:MSY1(YPL097W)|FD-Score:3.21|P-value:6.59E-4||SGD DESC:Mitochondrial tyrosyl-tRNA synthetase Gene:NPP1(YCR026C)|FD-Score:3.73|P-value:9.60E-5||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP1 has a paralog, NPP2, that arose from the whole genome duplication Gene:PTC7(YHR076W)|FD-Score:-3.76|P-value:8.57E-5||SGD DESC:Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope Gene:PYC2(YBR218C)|FD-Score:3.94|P-value:4.01E-5||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication Gene:RNP1(YLL046C)|FD-Score:-3.49|P-value:2.46E-4||SGD DESC:Ribonucleoprotein that contains two RNA recognition motifs (RRM) Gene:SET6(YPL165C)|FD-Score:-3.45|P-value:2.75E-4||SGD DESC:SET domain protein of unknown function; deletion heterozygote is sensitive to compounds that target ergosterol biosynthesis, may be involved in compound availability Gene:SLZ1(YNL196C_p)|FD-Score:3.14|P-value:8.40E-4||SGD DESC:Sporulation-specific protein with a leucine zipper motif Gene:SRS2(YJL092W)|FD-Score:4.17|P-value:1.52E-5||SGD DESC:DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability Gene:SUN4(YNL066W)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Cell wall protein related to glucanases, possibly involved in cell wall septation; member of the SUN family Gene:TGL5(YOR081C)|FD-Score:-4.1|P-value:2.03E-5||SGD DESC:Bifunctional enzyme with triacylglycerol lipase and lysophosphatidic acid acyltransferase activity; involved in triacylglycerol mobilization; localizes to lipid particles; potential Cdc28p substrate Gene:VAM3(YOR106W)|FD-Score:5.06|P-value:2.06E-7||SGD DESC:Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region Gene:YBR027C(YBR027C_d)|FD-Score:3.11|P-value:9.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR032W(YBR032W_d)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YBR238C(YBR238C)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YDR179W-A(YDR179W-A_p)|FD-Score:3.26|P-value:5.60E-4||SGD DESC:Putative protein of unknown function Gene:YDR278C(YDR278C_d)|FD-Score:-3.46|P-value:2.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER091C-A(YER091C-A_d)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL161W(YIL161W_p)|FD-Score:3.37|P-value:3.78E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; mRNA is enriched in Scp160p-associated mRNPs; YIL161W is a non-essential gene Gene:YJR018W(YJR018W_d)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR052C-A(YMR052C-A_d)|FD-Score:4.06|P-value:2.43E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNG1(YOR064C)|FD-Score:-3.29|P-value:4.96E-4||SGD DESC:Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3, has similarity to the human tumor suppressor ING1 Gene:YNL228W(YNL228W_d)|FD-Score:3.53|P-value:2.12E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF YNL227C/JJJ1 Gene:YNL285W(YNL285W_d)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR072W(YOR072W_d)|FD-Score:4.12|P-value:1.88E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YPL025C(YPL025C_d)|FD-Score:-3.86|P-value:5.57E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL247C(YPL247C_p)|FD-Score:4.74|P-value:1.08E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest Gene:ALE1(YOR175C)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Broad-specificity lysophospholipid acyltransferase, part of MBOAT family of membrane-bound O-acyltransferases; key component of Lands cycle; may have role in fatty acid exchange at sn-2 position of mature glycerophospholipids Gene:ATG21(YPL100W)|FD-Score:3.25|P-value:5.80E-4||SGD DESC:Phosphoinositide binding protein required for vesicle formation in the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein Gene:CAR1(YPL111W)|FD-Score:3.14|P-value:8.56E-4||SGD DESC:Arginase, responsible for arginine degradation, expression responds to both induction by arginine and nitrogen catabolite repression; disruption enhances freeze tolerance Gene:CIN1(YOR349W)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:COX9(YDL067C)|FD-Score:-3.15|P-value:8.28E-4||SGD DESC:Subunit VIIa of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:DAL3(YIR032C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Ureidoglycolate hydrolase, converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:DCC1(YCL016C)|FD-Score:3.8|P-value:7.22E-5||SGD DESC:Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance Gene:EST1(YLR233C)|FD-Score:-3.73|P-value:9.59E-5||SGD DESC:TLC1 RNA-associated factor involved in telomere length regulation as recruitment subunit of telomerase; has G-quadruplex promoting activity that is required for telomere elongation; possible role in activating telomere-bound Est2p-TLC1-RNA Gene:GLO2(YDR272W)|FD-Score:3.9|P-value:4.81E-5||SGD DESC:Cytoplasmic glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate Gene:GYP1(YOR070C)|FD-Score:-3.4|P-value:3.40E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HRK1(YOR267C)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Protein kinase; implicated in activation of the plasma membrane H(+)-ATPase Pma1p in response to glucose metabolism; plays a role in ion homeostasis; protein abundance increases in response to DNA replication stress Gene:IRC13(YOR235W_d)|FD-Score:3.31|P-value:4.65E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci Gene:IRC18(YJL037W)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Putative protein of unknown function; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRC7(YFR055W_p)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:Beta-lyase involved in the production of thiols; null mutant displays increased levels of spontaneous Rad52p foci; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner and by copper levels in a Mac1-dependent manner Gene:MSY1(YPL097W)|FD-Score:3.21|P-value:6.59E-4||SGD DESC:Mitochondrial tyrosyl-tRNA synthetase Gene:NPP1(YCR026C)|FD-Score:3.73|P-value:9.60E-5||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP1 has a paralog, NPP2, that arose from the whole genome duplication Gene:PTC7(YHR076W)|FD-Score:-3.76|P-value:8.57E-5||SGD DESC:Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope Gene:PYC2(YBR218C)|FD-Score:3.94|P-value:4.01E-5||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication Gene:RNP1(YLL046C)|FD-Score:-3.49|P-value:2.46E-4||SGD DESC:Ribonucleoprotein that contains two RNA recognition motifs (RRM) Gene:SET6(YPL165C)|FD-Score:-3.45|P-value:2.75E-4||SGD DESC:SET domain protein of unknown function; deletion heterozygote is sensitive to compounds that target ergosterol biosynthesis, may be involved in compound availability Gene:SLZ1(YNL196C_p)|FD-Score:3.14|P-value:8.40E-4||SGD DESC:Sporulation-specific protein with a leucine zipper motif Gene:SRS2(YJL092W)|FD-Score:4.17|P-value:1.52E-5||SGD DESC:DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability Gene:SUN4(YNL066W)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Cell wall protein related to glucanases, possibly involved in cell wall septation; member of the SUN family Gene:TGL5(YOR081C)|FD-Score:-4.1|P-value:2.03E-5||SGD DESC:Bifunctional enzyme with triacylglycerol lipase and lysophosphatidic acid acyltransferase activity; involved in triacylglycerol mobilization; localizes to lipid particles; potential Cdc28p substrate Gene:VAM3(YOR106W)|FD-Score:5.06|P-value:2.06E-7||SGD DESC:Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region Gene:YBR027C(YBR027C_d)|FD-Score:3.11|P-value:9.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR032W(YBR032W_d)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YBR238C(YBR238C)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YDR179W-A(YDR179W-A_p)|FD-Score:3.26|P-value:5.60E-4||SGD DESC:Putative protein of unknown function Gene:YDR278C(YDR278C_d)|FD-Score:-3.46|P-value:2.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER091C-A(YER091C-A_d)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL161W(YIL161W_p)|FD-Score:3.37|P-value:3.78E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; mRNA is enriched in Scp160p-associated mRNPs; YIL161W is a non-essential gene Gene:YJR018W(YJR018W_d)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR052C-A(YMR052C-A_d)|FD-Score:4.06|P-value:2.43E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNG1(YOR064C)|FD-Score:-3.29|P-value:4.96E-4||SGD DESC:Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3, has similarity to the human tumor suppressor ING1 Gene:YNL228W(YNL228W_d)|FD-Score:3.53|P-value:2.12E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF YNL227C/JJJ1 Gene:YNL285W(YNL285W_d)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR072W(YOR072W_d)|FD-Score:4.12|P-value:1.88E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YPL025C(YPL025C_d)|FD-Score:-3.86|P-value:5.57E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL247C(YPL247C_p)|FD-Score:4.74|P-value:1.08E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR033W5.052.25E-70.58RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YOR272W4.651.68E-60.58YTM1Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required for maturation of the large ribosomal subunit; has seven C-terminal WD repeats
YPR136C_d4.072.40E-50.21YPR136C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9
YKL028W3.855.81E-50.01TFA1TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening
YPL237W3.855.95E-50.07SUI3Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding
YKR062W3.787.85E-50.21TFA2TFIIE small subunit, involved in RNA polymerase II transcription initiation
YFR003C3.571.80E-40.08YPI1Regulatory subunit of the type I protein phosphatase (PP1) Glc7p; Glc7p participates in the regulation of a variety of metabolic processes including mitosis and glycogen metabolism; in vitro evidence suggests Ypi1p is an inhibitor of Glc7p while in vivo evidence suggests it is an activator; overproduction causes decreased cellular content of glycogen; partial depletion causes lithium sensitivity, while overproduction confers lithium-tolerance
YOR103C3.492.43E-40.14OST2Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins
YOL120C3.344.14E-40.17RPL18ARibosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication
YKL078W3.177.67E-40.03DHR2Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis
YBR142W3.148.39E-40.23MAK5Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits
YLR009W2.910.001810.10RLP24Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp
YLR230W_d2.810.002500.02YLR230W_dDubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity
YLR075W2.780.002690.00RPL10Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects
YHL015W2.780.002710.11RPS20Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOR106W5.062.06E-7VAM3Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region
YPL247C_p4.741.08E-6YPL247C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest
YJL092W4.171.52E-5SRS2DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability
YOR072W_d4.121.88E-5YOR072W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive
YMR052C-A_d4.062.43E-5YMR052C-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YBR218C3.944.01E-5PYC2Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication
YDR272W3.904.81E-5GLO2Cytoplasmic glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate
YCL016C3.807.22E-5DCC1Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance
YCR026C3.739.60E-5NPP1Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP1 has a paralog, NPP2, that arose from the whole genome duplication
YOR267C3.711.03E-4HRK1Protein kinase; implicated in activation of the plasma membrane H(+)-ATPase Pma1p in response to glucose metabolism; plays a role in ion homeostasis; protein abundance increases in response to DNA replication stress
YNL066W3.661.25E-4SUN4Cell wall protein related to glucanases, possibly involved in cell wall septation; member of the SUN family
YNL285W_d3.641.36E-4YNL285W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YJL037W3.631.44E-4IRC18Putative protein of unknown function; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52p foci
YIR032C3.611.53E-4DAL3Ureidoglycolate hydrolase, converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression sensitive to nitrogen catabolite repression
YOR175C3.591.63E-4ALE1Broad-specificity lysophospholipid acyltransferase, part of MBOAT family of membrane-bound O-acyltransferases; key component of Lands cycle; may have role in fatty acid exchange at sn-2 position of mature glycerophospholipids

GO enrichment analysis for SGTC_2654
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1032.53E-15SGTC_14464239-0190 62.0 μMChemDiv (Drug-like library)13400850.0615385
0.0961.84E-13SGTC_21866124449 200.0 μMChembridge (Fragment library)38341820.0243902RSC complex & mRNA processing
0.0921.25E-12SGTC_9701315-0089 19.5 μMChemDiv (Drug-like library)68110830.0378788RSC & ERG11
0.0863.92E-11SGTC_9721319-0219 195.0 μMChemDiv (Drug-like library)239037020.0428571
0.0759.78E-9SGTC_30199081971 49.5 μMChembridge (Drug-like library)164518030.03125
0.0741.38E-8SGTC_27827736369 71.4 μMChembridge (Drug-like library)29508360.0487805
0.0722.83E-8SGTC_8251988-2224 89.6 μMChemDiv (Drug-like library)11808590.0551181cell wall signaling
0.0723.75E-8SGTC_643k048-0106 28.4 μMChemDiv (Drug-like library)67639200.0241935RSC & ERG11
0.0681.62E-7SGTC_22297354935 190.0 μMChembridge (Fragment library)3181120.00854701RSC complex & mRNA processing
0.0681.58E-7SGTC_7093807-1509 243.0 μMChemDiv (Drug-like library)18866770.016129RSC complex & mRNA processing
0.0681.97E-7SGTC_31309127414 49.5 μMChembridge (Drug-like library)14946460.0373134
0.0682.01E-7SGTC_23489022543 33.4 μMChembridge (Fragment library)31626780.042735mitochondrial processes
0.0672.88E-7SGTC_1722st034304 3.7 μMTimTec (Natural product derivative library)40361480.027027mitochondrial processes
0.0655.14E-7SGTC_1652st011932 78.0 μMTimTec (Natural product derivative library)15510760.0756303RSC complex & mRNA processing
0.0656.85E-7SGTC_2534helenine 20.1 μMMicrosource (Natural product library)727240.0956522RSC complex & mRNA processing

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1630st00520931.3 μM0.1824823367314TimTec (Natural product derivative library)639.904746.46507
SGTC_2665lovastatin100 μM0.17653232Miscellaneous404.539644.21815
SGTC_504lovastatin124 μM0.17653232ICCB bioactive library404.539644.21815
SGTC_1545lovastatin49.4 μM0.17612923612TimTec (Pure natural product library)404.539644.21815
SGTC_1812st0520574.33 μM0.1753645418TimTec (Natural product derivative library)422.664224.01203calcium & mitochondrial duress
SGTC_2074185-00104.49 μM0.1683761753ChemDiv (Drug-like library)427.66245.12413endomembrane recycling
SGTC_1609st00157446.4 μM0.1626023960012TimTec (Natural product derivative library)430.576923.5615
SGTC_1715st03216010.23 μM0.1596645289825TimTec (Natural product derivative library)371.513022.21813
SGTC_1580hydrocortisone 21-hemisuccinate43.2 μM0.1562516623TimTec (Pure natural product library)462.532661.50738
SGTC_1578josamycin24.2 μM0.155285282165TimTec (Pure natural product library)827.994962.957316
SGTC_1579chlormadinone acetate49.4 μM0.15126145114258Miscellaneous448.979523.70605
SGTC_2649anisomycin9.56 μM0.1481486610309ICCB bioactive library235.278980.89324