pseudo-anisatin

A neurotoxin.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2656
Screen concentration 100.0 μM
Source Microsource (Natural product library)
PubChem CID 6708809
SMILES CC1CC(C2(C13CC(=O)C(C2(COC(=O)C3)C)(C)O)O)O
Standardized SMILES CC1CC(O)C2(O)C3(C)COC(=O)CC12CC(=O)C3(C)O
Molecular weight 298.3316
ALogP -0.76
H-bond donor count 3
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.8
% growth inhibition (Hom. pool) 2.64


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6708809
Download HIP data (tab-delimited text)  (excel)
Gene:GCD10(YNL062C)|FD-Score:3.33|P-value:4.27E-4|Clearance:0.4||SGD DESC:Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression Gene:PRP3(YDR473C)|FD-Score:3.2|P-value:6.84E-4|Clearance:0.4||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:GCD10(YNL062C)|FD-Score:3.33|P-value:4.27E-4|Clearance:0.4||SGD DESC:Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression Gene:PRP3(YDR473C)|FD-Score:3.2|P-value:6.84E-4|Clearance:0.4||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6708809
Download HOP data (tab-delimited text)  (excel)
Gene:CAR2(YLR438W)|FD-Score:3.23|P-value:6.24E-4||SGD DESC:L-ornithine transaminase (OTAse); catalyzes the second step of arginine degradation, expression is dually-regulated by allophanate induction and a specific arginine induction process; not nitrogen catabolite repression sensitive; protein abundance increases in response to DNA replication stress Gene:CBC2(YPL178W)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Small subunit of the heterodimeric cap binding complex that also contains Sto1p, component of the spliceosomal commitment complex; interacts with Npl3p, possibly to package mRNA for export from the nucleus; contains an RNA-binding motif Gene:DGA1(YOR245C)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Diacylglycerol acyltransferase, catalyzes the terminal step of triacylglycerol (TAG) formation, acylates diacylglycerol using acyl-CoA as an acyl donor, localized to lipid particles Gene:EMC6(YLL014W)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm F33D4.7/EMC-6, fly CG11781, human TMEM93 Gene:IME4(YGL192W)|FD-Score:3.15|P-value:8.21E-4||SGD DESC:Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids Gene:LDB7(YBL006C)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:MNN11(YJL183W)|FD-Score:-3.45|P-value:2.79E-4||SGD DESC:Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p Gene:MSN1(YOL116W)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:SYG1(YIL047C)|FD-Score:-3.29|P-value:4.99E-4||SGD DESC:Plasma membrane protein of unknown function; truncation and overexpression suppresses lethality of G-alpha protein deficiency Gene:YGL117W(YGL117W_p)|FD-Score:-3.36|P-value:3.88E-4||SGD DESC:Putative protein of unknown function Gene:YKL031W(YKL031W_d)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species Gene:YKR005C(YKR005C_p)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Putative protein of unknown function Gene:YMD8(YML038C)|FD-Score:3.26|P-value:5.54E-4||SGD DESC:Putative nucleotide sugar transporter, has similarity to Vrg4p Gene:YMR326C(YMR326C_d)|FD-Score:-3.22|P-value:6.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the telomere on the right arm of chromosome 13 Gene:CAR2(YLR438W)|FD-Score:3.23|P-value:6.24E-4||SGD DESC:L-ornithine transaminase (OTAse); catalyzes the second step of arginine degradation, expression is dually-regulated by allophanate induction and a specific arginine induction process; not nitrogen catabolite repression sensitive; protein abundance increases in response to DNA replication stress Gene:CBC2(YPL178W)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Small subunit of the heterodimeric cap binding complex that also contains Sto1p, component of the spliceosomal commitment complex; interacts with Npl3p, possibly to package mRNA for export from the nucleus; contains an RNA-binding motif Gene:DGA1(YOR245C)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Diacylglycerol acyltransferase, catalyzes the terminal step of triacylglycerol (TAG) formation, acylates diacylglycerol using acyl-CoA as an acyl donor, localized to lipid particles Gene:EMC6(YLL014W)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm F33D4.7/EMC-6, fly CG11781, human TMEM93 Gene:IME4(YGL192W)|FD-Score:3.15|P-value:8.21E-4||SGD DESC:Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids Gene:LDB7(YBL006C)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:MNN11(YJL183W)|FD-Score:-3.45|P-value:2.79E-4||SGD DESC:Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p Gene:MSN1(YOL116W)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:SYG1(YIL047C)|FD-Score:-3.29|P-value:4.99E-4||SGD DESC:Plasma membrane protein of unknown function; truncation and overexpression suppresses lethality of G-alpha protein deficiency Gene:YGL117W(YGL117W_p)|FD-Score:-3.36|P-value:3.88E-4||SGD DESC:Putative protein of unknown function Gene:YKL031W(YKL031W_d)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species Gene:YKR005C(YKR005C_p)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Putative protein of unknown function Gene:YMD8(YML038C)|FD-Score:3.26|P-value:5.54E-4||SGD DESC:Putative nucleotide sugar transporter, has similarity to Vrg4p Gene:YMR326C(YMR326C_d)|FD-Score:-3.22|P-value:6.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the telomere on the right arm of chromosome 13

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YNL062C3.334.27E-40.40GCD10Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression
YDR473C3.206.84E-40.40PRP3Splicing factor, component of the U4/U6-U5 snRNP complex
YLR339C_d2.800.002520.04YLR339C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPP0
YGL171W2.760.002870.04ROK1RNA-dependent ATPase; involved in pre-rRNA processing at sites A0, A1, and A2, and in control of cell cycle progression; contains two upstream open reading frames (uORFs) in 5' untranslated region which regulate translation
YPR137W2.720.003270.04RRP9Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein
YOR272W2.680.003680.02YTM1Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required for maturation of the large ribosomal subunit; has seven C-terminal WD repeats
YOR122C2.660.003860.03PFY1Profilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress
YKR081C2.640.004200.10RPF2Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles
YLR147C2.540.005590.27SMD3Core Sm protein Sm D3; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3
YDR087C2.270.011700.05RRP1Essential evolutionarily conserved nucleolar protein necessary for biogenesis of 60S ribosomal subunits and processing of pre-rRNAs to mature rRNAs, associated with several distinct 66S pre-ribosomal particles
YFR003C2.220.013100.02YPI1Regulatory subunit of the type I protein phosphatase (PP1) Glc7p; Glc7p participates in the regulation of a variety of metabolic processes including mitosis and glycogen metabolism; in vitro evidence suggests Ypi1p is an inhibitor of Glc7p while in vivo evidence suggests it is an activator; overproduction causes decreased cellular content of glycogen; partial depletion causes lithium sensitivity, while overproduction confers lithium-tolerance
YCR057C2.210.013700.05PWP2Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis
YLR167W2.150.015600.02RPS31Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog
YGL145W2.140.016200.01TIP20Peripheral membrane protein required for fusion of COPI vesicles with the ER; prohibits back-fusion of COPII vesicles with the ER; forms a tethering complex with Sec39p and Dsl1p that interacts with ER SNAREs Sec20p and Use1p
YOR259C2.130.016700.00RPT4One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBL006C3.671.21E-4LDB7Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions
YKL031W_d3.571.76E-4YKL031W_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species
YLL014W3.542.02E-4EMC6Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm F33D4.7/EMC-6, fly CG11781, human TMEM93
YKR005C_p3.502.34E-4YKR005C_pPutative protein of unknown function
YOR245C3.492.44E-4DGA1Diacylglycerol acyltransferase, catalyzes the terminal step of triacylglycerol (TAG) formation, acylates diacylglycerol using acyl-CoA as an acyl donor, localized to lipid particles
YPL178W3.442.88E-4CBC2Small subunit of the heterodimeric cap binding complex that also contains Sto1p, component of the spliceosomal commitment complex; interacts with Npl3p, possibly to package mRNA for export from the nucleus; contains an RNA-binding motif
YML038C3.265.54E-4YMD8Putative nucleotide sugar transporter, has similarity to Vrg4p
YLR438W3.236.24E-4CAR2L-ornithine transaminase (OTAse); catalyzes the second step of arginine degradation, expression is dually-regulated by allophanate induction and a specific arginine induction process; not nitrogen catabolite repression sensitive; protein abundance increases in response to DNA replication stress
YOL116W3.226.42E-4MSN1Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress
YGL192W3.158.21E-4IME4Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids
YGR097W3.080.00104ASK10Component of RNA polymerase II holoenzyme; phosphorylated in response to oxidative stress; has a role in destruction of Ssn8p; proposed to function in activation of the glycerol channel Fps1p; ASK10 has a paralog, RGC1, that arose from the whole genome duplication
YJL179W3.030.00123PFD1Subunit of heterohexameric prefoldin, which binds cytosolic chaperonin and transfers target proteins to it; involved in the biogenesis of actin and of alpha- and gamma-tubulin
YFL034C-A2.890.00192RPL22BRibosomal 60S subunit protein L22B; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22B has a paralog, RPL22A, that arose from the whole genome duplication
YGR056W2.870.00204RSC1Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook
YJR037W_d2.800.00255YJR037W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene HUL4/YJR036C; deletion mutant has decreased spore survival in Drosophila feces

GO enrichment analysis for SGTC_2656
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1016.13E-15SGTC_31309127414 49.5 μMChembridge (Drug-like library)14946460.0344828
0.0961.29E-13SGTC_8630466-0191 129.0 μMChemDiv (Drug-like library)7651600.057971cell wall
0.0898.80E-12SGTC_32859112818 20.9 μMChembridge (Drug-like library)170179470.0481928cell wall
0.0815.11E-10SGTC_29799012552 45.5 μMChembridge (Drug-like library)171009050.0277778
0.0791.16E-9SGTC_5914299-0174 154.0 μMChemDiv (Drug-like library)55090880.0666667
0.0765.28E-9SGTC_22157188065 200.0 μMChembridge (Fragment library)9402170.0327869
0.0757.68E-9SGTC_33219137793 26.8 μMChembridge (Drug-like library)49041920.0232558cell wall
0.0759.19E-9SGTC_1961st076550 68.7 μMTimTec (Natural product derivative library)15793370.0675676
0.0741.13E-8SGTC_1140364-0001 490.3 μMChemDiv (Drug-like library)40036550.0684932
0.0741.35E-8SGTC_31349127454 49.5 μMChembridge (Drug-like library)242628200.0588235
0.0741.36E-8SGTC_33569151811 4.5 μMChembridge (Drug-like library)18592540.0227273cell wall
0.0731.72E-8SGTC_32669136860 49.5 μMChembridge (Drug-like library)48712710.0238095cell wall
0.0732.05E-8SGTC_2529gangaleoidin 93.6 μMMicrosource (Natural product library)45651830.0759494
0.0723.24E-8SGTC_22287354201 200.0 μMChembridge (Fragment library)7534510.0588235endomembrane recycling
0.0672.34E-7SGTC_26682',4'-dihydroxychalcone 15.1 μMTimTec (Pure natural product library)53572180.046875

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1572epiandrosterone68.9 μM0.1875441302TimTec (Pure natural product library)290.44033.58812
SGTC_2540epiandrosterone100 μM0.1875441302TimTec (Pure natural product library)290.44033.58812
SGTC_2584androsterone48.74 μM0.18752754130Microsource (Natural product library)290.44033.58812cell wall
SGTC_2415hr heat shock (37°C) + cantharidin155.45 μM0.182545ICCB bioactive library196.199880.71704cell wall signaling
SGTC_2489cantharidin73.42 μM0.182545ICCB bioactive library196.199880.71704cell wall signaling
SGTC_408cantharidin100 μM0.182545ICCB bioactive library196.199880.71704cell wall signaling
SGTC_422cantharidin155 μM0.182545ICCB bioactive library196.199880.71704cell wall signaling
SGTC_1570verbenone133 μM0.17307729025TimTec (Pure natural product library)150.217562.08201
SGTC_25383',7'-epoxycaryophyllan-6-one100 μM0.1694924330530Microsource (Natural product library)236.349862.59902
SGTC_2405hr heat shock (37°C) + Streptovitacin300 μM0.16923191467Miscellaneous297.34682-0.35135heat shock/prefoldin
SGTC_421streptovitacin30 mM0.16923191467Miscellaneous297.34682-0.35135
SGTC_2650prasterone100 μM0.16923116759247Microsource (Natural product library)288.424423.33812
SGTC_2758dehydroepiandrosterone69.35 μM0.1692315881Miscellaneous288.424423.33812