nystatin FDA approved compound

(21E,23E,25E,27E,31E,33E)-20-[(2R,3S,4S,5S,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl]oxy-4,6,8,11,12,16,18,36-octahydroxy-35,37,38-trimethyl-2,14-dioxo-1-oxacyclooctatriaconta-21,23,25,27,31,33-hexaene-17-carboxylic acid

Nystatin is a polyene antifungal that binds irreversibly to ergosterol, resulting in disruption of membrane integrity and ultimately cell death.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2657
Screen concentration 74.4 μM
Source Miscellaneous
PubChem CID 11468707
SMILES CC1C=CC=CCCC=CC=CC=CC=CC(CC(C(C(CC(=O)CC(C(CCC(CC(CC(CC(=O)OC(C(C1O)C)C)O)O)O)O)O)O)C(=O)O)O)OC2C(C(C(C(O2)C)O)N)O
Standardized SMILES CC1OC(=O)CC(O)CC(O)CC(O)CCC(O)C(O)CC(=O)CC(O)C(C(O)CC(OC2OC(C)C(O)C(N)C2O)C=CC=CC=CC=CCCC=CC=CC(C)C(O)C1C)C(=O)O
Molecular weight 926.0949
ALogP -2.6
H-bond donor count 12
H-bond acceptor count 18
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 7.06
% growth inhibition (Hom. pool) 2.64


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 11468707
Download HIP data (tab-delimited text)  (excel)
Gene:ACT1(YFL039C)|FD-Score:3.78|P-value:7.71E-5|Clearance:0.03||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:FCP1(YMR277W)|FD-Score:3.73|P-value:9.68E-5|Clearance:0.16||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:GAA1(YLR088W)|FD-Score:4|P-value:3.10E-5|Clearance:0.19||SGD DESC:Subunit of the GPI (glycosylphosphatidylinositol):protein transamidase complex, removes the GPI-anchoring signal and attaches GPI to proteins in the ER Gene:GLN4(YOR168W)|FD-Score:3.36|P-value:3.84E-4|Clearance:0.2||SGD DESC:Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions Gene:HRP1(YOL123W)|FD-Score:-3.33|P-value:4.33E-4|Clearance:0||SGD DESC:Subunit of cleavage factor I, a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences Gene:KRI1(YNL308C)|FD-Score:3.82|P-value:6.78E-5|Clearance:0.03||SGD DESC:Essential nucleolar protein required for 40S ribosome biogenesis; associate with snR30; physically and functionally interacts with Krr1p Gene:NOP19(YGR251W)|FD-Score:3.74|P-value:9.03E-5|Clearance:0.02||SGD DESC:Ribosome biogenesis factor; nucleolar protein associated with pre-rRNA components of the 90S preribosome, required for cleavage of pre-rRNA at A0, A1 and A2 sites; interacts with RNA helicase Dhr2p and RNA helicase-like protein Utp25p; required for incorporation of Utp25p into preribosomes Gene:NPA3(YJR072C)|FD-Score:3.57|P-value:1.79E-4|Clearance:0.2||SGD DESC:Member of the conserved GPN-loop GTPase family; has a role in transport of RNA polymerase II to the nucleus; exhibits GTP-dependent binding to PolII; has ATPase activity; involved in sister chromatid cohesion; phosphorylated by the Pcl1p-Pho85p kinase complex; human homolog XAB1 interacts with human RNA polymerase II; protein abundance increases in response to DNA replication stress Gene:NUP85(YJR042W)|FD-Score:4.32|P-value:7.83E-6|Clearance:0.31||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75 Gene:PBN1(YCL052C)|FD-Score:-3.1|P-value:9.66E-4|Clearance:0||SGD DESC:Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X Gene:RER2(YBR002C)|FD-Score:3.75|P-value:8.76E-5|Clearance:0.01||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RRN6(YBL014C)|FD-Score:3.15|P-value:8.25E-4|Clearance:0.03||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:SEC1(YDR164C)|FD-Score:-3.64|P-value:1.34E-4|Clearance:0||SGD DESC:Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function Gene:SEC7(YDR170C)|FD-Score:4.7|P-value:1.33E-6|Clearance:0.38||SGD DESC:Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles Gene:SMC4(YLR086W)|FD-Score:-3.69|P-value:1.11E-4|Clearance:0||SGD DESC:Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate Gene:SNP1(YIL061C)|FD-Score:3.11|P-value:9.21E-4|Clearance:0.04||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress Gene:SPT16(YGL207W)|FD-Score:-3.32|P-value:4.46E-4|Clearance:0||SGD DESC:Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; some mutations cause reduced nucleosome occupancy over highly transcribed regions of the yeast genome Gene:TOP2(YNL088W)|FD-Score:3.16|P-value:7.82E-4|Clearance:0.01||SGD DESC:Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:UFE1(YOR075W)|FD-Score:3.15|P-value:8.17E-4|Clearance:0||SGD DESC:t-SNARE required for retrograde vesicular traffic and homotypic ER membrane fusion; forms a complex with the SNAREs Sec22p, Sec20p and Use1p to mediate fusion of Golgi-derived vesicles at the ER Gene:YLR076C(YLR076C_d)|FD-Score:3.15|P-value:8.23E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 Gene:YLR230W(YLR230W_d)|FD-Score:-3.14|P-value:8.55E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity Gene:ACT1(YFL039C)|FD-Score:3.78|P-value:7.71E-5|Clearance:0.03||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:FCP1(YMR277W)|FD-Score:3.73|P-value:9.68E-5|Clearance:0.16||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:GAA1(YLR088W)|FD-Score:4|P-value:3.10E-5|Clearance:0.19||SGD DESC:Subunit of the GPI (glycosylphosphatidylinositol):protein transamidase complex, removes the GPI-anchoring signal and attaches GPI to proteins in the ER Gene:GLN4(YOR168W)|FD-Score:3.36|P-value:3.84E-4|Clearance:0.2||SGD DESC:Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions Gene:HRP1(YOL123W)|FD-Score:-3.33|P-value:4.33E-4|Clearance:0||SGD DESC:Subunit of cleavage factor I, a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences Gene:KRI1(YNL308C)|FD-Score:3.82|P-value:6.78E-5|Clearance:0.03||SGD DESC:Essential nucleolar protein required for 40S ribosome biogenesis; associate with snR30; physically and functionally interacts with Krr1p Gene:NOP19(YGR251W)|FD-Score:3.74|P-value:9.03E-5|Clearance:0.02||SGD DESC:Ribosome biogenesis factor; nucleolar protein associated with pre-rRNA components of the 90S preribosome, required for cleavage of pre-rRNA at A0, A1 and A2 sites; interacts with RNA helicase Dhr2p and RNA helicase-like protein Utp25p; required for incorporation of Utp25p into preribosomes Gene:NPA3(YJR072C)|FD-Score:3.57|P-value:1.79E-4|Clearance:0.2||SGD DESC:Member of the conserved GPN-loop GTPase family; has a role in transport of RNA polymerase II to the nucleus; exhibits GTP-dependent binding to PolII; has ATPase activity; involved in sister chromatid cohesion; phosphorylated by the Pcl1p-Pho85p kinase complex; human homolog XAB1 interacts with human RNA polymerase II; protein abundance increases in response to DNA replication stress Gene:NUP85(YJR042W)|FD-Score:4.32|P-value:7.83E-6|Clearance:0.31||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75 Gene:PBN1(YCL052C)|FD-Score:-3.1|P-value:9.66E-4|Clearance:0||SGD DESC:Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X Gene:RER2(YBR002C)|FD-Score:3.75|P-value:8.76E-5|Clearance:0.01||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RRN6(YBL014C)|FD-Score:3.15|P-value:8.25E-4|Clearance:0.03||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:SEC1(YDR164C)|FD-Score:-3.64|P-value:1.34E-4|Clearance:0||SGD DESC:Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function Gene:SEC7(YDR170C)|FD-Score:4.7|P-value:1.33E-6|Clearance:0.38||SGD DESC:Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles Gene:SMC4(YLR086W)|FD-Score:-3.69|P-value:1.11E-4|Clearance:0||SGD DESC:Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate Gene:SNP1(YIL061C)|FD-Score:3.11|P-value:9.21E-4|Clearance:0.04||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress Gene:SPT16(YGL207W)|FD-Score:-3.32|P-value:4.46E-4|Clearance:0||SGD DESC:Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; some mutations cause reduced nucleosome occupancy over highly transcribed regions of the yeast genome Gene:TOP2(YNL088W)|FD-Score:3.16|P-value:7.82E-4|Clearance:0.01||SGD DESC:Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:UFE1(YOR075W)|FD-Score:3.15|P-value:8.17E-4|Clearance:0||SGD DESC:t-SNARE required for retrograde vesicular traffic and homotypic ER membrane fusion; forms a complex with the SNAREs Sec22p, Sec20p and Use1p to mediate fusion of Golgi-derived vesicles at the ER Gene:YLR076C(YLR076C_d)|FD-Score:3.15|P-value:8.23E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 Gene:YLR230W(YLR230W_d)|FD-Score:-3.14|P-value:8.55E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 11468707
Download HOP data (tab-delimited text)  (excel)
Gene:ACE2(YLR131C)|FD-Score:4.77|P-value:9.34E-7||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:AGA2(YGL032C)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Adhesion subunit of a-agglutinin of a-cells, C-terminal sequence acts as a ligand for alpha-agglutinin (Sag1p) during agglutination, modified with O-linked oligomannosyl chains, linked to anchorage subunit Aga1p via two disulfide bonds Gene:AIM13(YFR011C)|FD-Score:-4.4|P-value:5.38E-6||SGD DESC:Mitochondrial protein peripheral to the inner membrane; component of the mitochondrial inner membrane organizing system (MINOS, MitOS, or MICOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:ALE1(YOR175C)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Broad-specificity lysophospholipid acyltransferase, part of MBOAT family of membrane-bound O-acyltransferases; key component of Lands cycle; may have role in fatty acid exchange at sn-2 position of mature glycerophospholipids Gene:ALG12(YNR030W)|FD-Score:4|P-value:3.16E-5||SGD DESC:Alpha-1,6-mannosyltransferase localized to the ER; responsible for the addition of the alpha-1,6 mannose to dolichol-linked Man7GlcNAc2, acts in the dolichol pathway for N-glycosylation Gene:BNI4(YNL233W)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p Gene:BPH1(YCR032W)|FD-Score:3.22|P-value:6.35E-4||SGD DESC:Protein homologous to Chediak-Higashi syndrome and Beige proteins; both of which are implicated in disease syndromes in human and mouse, respectively, due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting Gene:CDC10(YCR002C)|FD-Score:3.3|P-value:4.90E-4||SGD DESC:Component of the septin ring, required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells; N-terminus interacts with phosphatidylinositol-4,5-bisphosphate; protein abundance increases under DNA damage stress Gene:CNE1(YAL058W)|FD-Score:3.31|P-value:4.64E-4||SGD DESC:Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast Gene:DIC1(YLR348C)|FD-Score:3.74|P-value:9.26E-5||SGD DESC:Mitochondrial dicarboxylate carrier, integral membrane protein, catalyzes a dicarboxylate-phosphate exchange across the inner mitochondrial membrane, transports cytoplasmic dicarboxylates into the mitochondrial matrix Gene:EDC3(YEL015W)|FD-Score:3.1|P-value:9.73E-4||SGD DESC:Non-essential conserved protein with a role in mRNA decapping; specifically affects the function of the decapping enzyme Dcp1p; localizes to cytoplasmic mRNA processing bodies; forms cytoplasmic foci upon DNA replication stress Gene:EMC1(YCL045C)|FD-Score:3.27|P-value:5.44E-4||SGD DESC:Member of conserved endoplasmic reticulum membrane complex; involved in efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; interacts with Gal80p; homologous to worm H17B01.4/EMC-1, fly CG2943, and human KIAA0090 Gene:FET4(YMR319C)|FD-Score:6.14|P-value:4.22E-10||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:GCY1(YOR120W)|FD-Score:4.79|P-value:8.46E-7||SGD DESC:Glycerol dehydrogenase;involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication Gene:GUP1(YGL084C)|FD-Score:3.76|P-value:8.56E-5||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:KAP123(YER110C)|FD-Score:3.16|P-value:8.02E-4||SGD DESC:Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1 Gene:KTI12(YKL110C)|FD-Score:3.22|P-value:6.52E-4||SGD DESC:Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p Gene:MIT1(YEL007W)|FD-Score:3.61|P-value:1.56E-4||SGD DESC:Transcriptional regulator of pseudohyphal growth; protein with sequence similarity to S. pombe gti1+ (gluconate transport inducer 1) and C. albicans Wor1 Gene:MNN2(YBR015C)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MTD1(YKR080W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase, plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline Gene:NCA3(YJL116C)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Protein involved in mitochondrion organization; functions with Nca2p to regulate mitochondrial expression of subunits 6 (Atp6p) and 8 (Atp8p) of the Fo-F1 ATP synthase; member of the SUN family; expression induced in cells treated with the mycotoxin patulin; NCA3 has a paralog, UTH1, that arose from the whole genome duplication Gene:PCI8(YIL071C)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain Gene:PCL7(YIL050W)|FD-Score:3.1|P-value:9.64E-4||SGD DESC:Pho85p cyclin of the Pho80p subfamily, forms a functional kinase complex with Pho85p which phosphorylates Mmr1p and is regulated by Pho81p; involved in glycogen metabolism, expression is cell-cycle regulated Gene:PER1(YCR044C)|FD-Score:-4.05|P-value:2.60E-5||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PLP1(YDR183W)|FD-Score:3.44|P-value:2.96E-4||SGD DESC:Protein that interacts with CCT (chaperonin containing TCP-1) complex and has a role in actin and tubulin folding; has weak similarity to phosducins, which are G-protein regulators Gene:RPH1(YER169W)|FD-Score:3.94|P-value:4.09E-5||SGD DESC:JmjC domain-containing histone demethylase; specifically demethylates H3K36 tri- and dimethyl modification states; associates with actively transcribed (RNA polymerase II) regions in vivo and specifically targets H3K36 in its trimethylation state as its substrate; transcriptional repressor of PHR1; Rph1p phosphorylation during DNA damage is under control of the MEC1-RAD53 pathway; RPH1 has a paralog, GIS1, that arose from the whole genome duplication Gene:RPL35B(YDL136W)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RPS18B(YML026C)|FD-Score:3.88|P-value:5.32E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18B has a paralog, RPS18A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SAP4(YGL229C)|FD-Score:3.19|P-value:7.20E-4||SGD DESC:Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p Gene:SCP1(YOR367W)|FD-Score:-3.13|P-value:8.72E-4||SGD DESC:Component of yeast cortical actin cytoskeleton, binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin Gene:SEY1(YOR165W)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Dynamin-like GTPase that mediates homotypic ER fusion; has a role in ER morphology; interacts physically and genetically with Yop1p and Rtn1p; functional ortholog of the human atlastin ATL1, defects in which cause a form of the human disease hereditary spastic paraplegia; homolog of Arabidopsis RHD3 Gene:SNG1(YGR197C)|FD-Score:3.11|P-value:9.42E-4||SGD DESC:Protein involved in resistance to nitrosoguanidine (MNNG) and 6-azauracil (6-AU); expression is regulated by transcription factors involved in multidrug resistance Gene:STF1(YDL130W-A)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Protein involved in regulation of the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p act as stabilizing factors that enhance inhibitory action of the Inh1p protein; protein abundance increases in response to DNA replication stress Gene:SWI6(YLR182W)|FD-Score:-3.19|P-value:7.15E-4||SGD DESC:Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators Gene:TSC3(YBR058C-A)|FD-Score:-3.33|P-value:4.35E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:YBR134W(YBR134W_d)|FD-Score:3.1|P-value:9.64E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDL057W(YDL057W_p)|FD-Score:-3.44|P-value:2.91E-4||SGD DESC:Putative protein of unknown function; YDL057W is not an essential gene Gene:YER097W(YER097W_d)|FD-Score:5.03|P-value:2.42E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER135C(YER135C_d)|FD-Score:3.83|P-value:6.45E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; YER135C is not an essential gene Gene:YFR018C(YFR018C_p)|FD-Score:5.04|P-value:2.39E-7||SGD DESC:Putative protein of unknown function Gene:YGL072C(YGL072C_d)|FD-Score:3.95|P-value:3.83E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YHR035W(YHR035W_p)|FD-Score:-3.55|P-value:1.92E-4||SGD DESC:Putative protein of unknown function; not an essential gene Gene:YIL025C(YIL025C_d)|FD-Score:3.22|P-value:6.48E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR111C(YJR111C_p)|FD-Score:3.96|P-value:3.68E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria Gene:YLR412C-A(YLR412C-A_p)|FD-Score:4.45|P-value:4.25E-6||SGD DESC:Putative protein of unknown function Gene:YML009C-A(YML009C-A_d)|FD-Score:3.75|P-value:8.89E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML047W-A(YML047W-A_d)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL193W(YNL193W_p)|FD-Score:3.23|P-value:6.27E-4||SGD DESC:Putative protein of unknown function; exhibits a two-hybrid interaction with Yhr151cp in a large-scale analysis Gene:YNL285W(YNL285W_d)|FD-Score:5.44|P-value:2.62E-8||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR170W(YOR170W_d)|FD-Score:3.83|P-value:6.38E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene LCB4 Gene:YOR228C(YOR228C)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YPS3(YLR121C)|FD-Score:-3.98|P-value:3.44E-5||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:ACE2(YLR131C)|FD-Score:4.77|P-value:9.34E-7||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:AGA2(YGL032C)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Adhesion subunit of a-agglutinin of a-cells, C-terminal sequence acts as a ligand for alpha-agglutinin (Sag1p) during agglutination, modified with O-linked oligomannosyl chains, linked to anchorage subunit Aga1p via two disulfide bonds Gene:AIM13(YFR011C)|FD-Score:-4.4|P-value:5.38E-6||SGD DESC:Mitochondrial protein peripheral to the inner membrane; component of the mitochondrial inner membrane organizing system (MINOS, MitOS, or MICOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:ALE1(YOR175C)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Broad-specificity lysophospholipid acyltransferase, part of MBOAT family of membrane-bound O-acyltransferases; key component of Lands cycle; may have role in fatty acid exchange at sn-2 position of mature glycerophospholipids Gene:ALG12(YNR030W)|FD-Score:4|P-value:3.16E-5||SGD DESC:Alpha-1,6-mannosyltransferase localized to the ER; responsible for the addition of the alpha-1,6 mannose to dolichol-linked Man7GlcNAc2, acts in the dolichol pathway for N-glycosylation Gene:BNI4(YNL233W)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p Gene:BPH1(YCR032W)|FD-Score:3.22|P-value:6.35E-4||SGD DESC:Protein homologous to Chediak-Higashi syndrome and Beige proteins; both of which are implicated in disease syndromes in human and mouse, respectively, due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting Gene:CDC10(YCR002C)|FD-Score:3.3|P-value:4.90E-4||SGD DESC:Component of the septin ring, required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells; N-terminus interacts with phosphatidylinositol-4,5-bisphosphate; protein abundance increases under DNA damage stress Gene:CNE1(YAL058W)|FD-Score:3.31|P-value:4.64E-4||SGD DESC:Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast Gene:DIC1(YLR348C)|FD-Score:3.74|P-value:9.26E-5||SGD DESC:Mitochondrial dicarboxylate carrier, integral membrane protein, catalyzes a dicarboxylate-phosphate exchange across the inner mitochondrial membrane, transports cytoplasmic dicarboxylates into the mitochondrial matrix Gene:EDC3(YEL015W)|FD-Score:3.1|P-value:9.73E-4||SGD DESC:Non-essential conserved protein with a role in mRNA decapping; specifically affects the function of the decapping enzyme Dcp1p; localizes to cytoplasmic mRNA processing bodies; forms cytoplasmic foci upon DNA replication stress Gene:EMC1(YCL045C)|FD-Score:3.27|P-value:5.44E-4||SGD DESC:Member of conserved endoplasmic reticulum membrane complex; involved in efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; interacts with Gal80p; homologous to worm H17B01.4/EMC-1, fly CG2943, and human KIAA0090 Gene:FET4(YMR319C)|FD-Score:6.14|P-value:4.22E-10||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:GCY1(YOR120W)|FD-Score:4.79|P-value:8.46E-7||SGD DESC:Glycerol dehydrogenase;involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication Gene:GUP1(YGL084C)|FD-Score:3.76|P-value:8.56E-5||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:KAP123(YER110C)|FD-Score:3.16|P-value:8.02E-4||SGD DESC:Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1 Gene:KTI12(YKL110C)|FD-Score:3.22|P-value:6.52E-4||SGD DESC:Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p Gene:MIT1(YEL007W)|FD-Score:3.61|P-value:1.56E-4||SGD DESC:Transcriptional regulator of pseudohyphal growth; protein with sequence similarity to S. pombe gti1+ (gluconate transport inducer 1) and C. albicans Wor1 Gene:MNN2(YBR015C)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MTD1(YKR080W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase, plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline Gene:NCA3(YJL116C)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Protein involved in mitochondrion organization; functions with Nca2p to regulate mitochondrial expression of subunits 6 (Atp6p) and 8 (Atp8p) of the Fo-F1 ATP synthase; member of the SUN family; expression induced in cells treated with the mycotoxin patulin; NCA3 has a paralog, UTH1, that arose from the whole genome duplication Gene:PCI8(YIL071C)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain Gene:PCL7(YIL050W)|FD-Score:3.1|P-value:9.64E-4||SGD DESC:Pho85p cyclin of the Pho80p subfamily, forms a functional kinase complex with Pho85p which phosphorylates Mmr1p and is regulated by Pho81p; involved in glycogen metabolism, expression is cell-cycle regulated Gene:PER1(YCR044C)|FD-Score:-4.05|P-value:2.60E-5||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PLP1(YDR183W)|FD-Score:3.44|P-value:2.96E-4||SGD DESC:Protein that interacts with CCT (chaperonin containing TCP-1) complex and has a role in actin and tubulin folding; has weak similarity to phosducins, which are G-protein regulators Gene:RPH1(YER169W)|FD-Score:3.94|P-value:4.09E-5||SGD DESC:JmjC domain-containing histone demethylase; specifically demethylates H3K36 tri- and dimethyl modification states; associates with actively transcribed (RNA polymerase II) regions in vivo and specifically targets H3K36 in its trimethylation state as its substrate; transcriptional repressor of PHR1; Rph1p phosphorylation during DNA damage is under control of the MEC1-RAD53 pathway; RPH1 has a paralog, GIS1, that arose from the whole genome duplication Gene:RPL35B(YDL136W)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RPS18B(YML026C)|FD-Score:3.88|P-value:5.32E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18B has a paralog, RPS18A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SAP4(YGL229C)|FD-Score:3.19|P-value:7.20E-4||SGD DESC:Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p Gene:SCP1(YOR367W)|FD-Score:-3.13|P-value:8.72E-4||SGD DESC:Component of yeast cortical actin cytoskeleton, binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin Gene:SEY1(YOR165W)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Dynamin-like GTPase that mediates homotypic ER fusion; has a role in ER morphology; interacts physically and genetically with Yop1p and Rtn1p; functional ortholog of the human atlastin ATL1, defects in which cause a form of the human disease hereditary spastic paraplegia; homolog of Arabidopsis RHD3 Gene:SNG1(YGR197C)|FD-Score:3.11|P-value:9.42E-4||SGD DESC:Protein involved in resistance to nitrosoguanidine (MNNG) and 6-azauracil (6-AU); expression is regulated by transcription factors involved in multidrug resistance Gene:STF1(YDL130W-A)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Protein involved in regulation of the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p act as stabilizing factors that enhance inhibitory action of the Inh1p protein; protein abundance increases in response to DNA replication stress Gene:SWI6(YLR182W)|FD-Score:-3.19|P-value:7.15E-4||SGD DESC:Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators Gene:TSC3(YBR058C-A)|FD-Score:-3.33|P-value:4.35E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:YBR134W(YBR134W_d)|FD-Score:3.1|P-value:9.64E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDL057W(YDL057W_p)|FD-Score:-3.44|P-value:2.91E-4||SGD DESC:Putative protein of unknown function; YDL057W is not an essential gene Gene:YER097W(YER097W_d)|FD-Score:5.03|P-value:2.42E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER135C(YER135C_d)|FD-Score:3.83|P-value:6.45E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; YER135C is not an essential gene Gene:YFR018C(YFR018C_p)|FD-Score:5.04|P-value:2.39E-7||SGD DESC:Putative protein of unknown function Gene:YGL072C(YGL072C_d)|FD-Score:3.95|P-value:3.83E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YHR035W(YHR035W_p)|FD-Score:-3.55|P-value:1.92E-4||SGD DESC:Putative protein of unknown function; not an essential gene Gene:YIL025C(YIL025C_d)|FD-Score:3.22|P-value:6.48E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR111C(YJR111C_p)|FD-Score:3.96|P-value:3.68E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria Gene:YLR412C-A(YLR412C-A_p)|FD-Score:4.45|P-value:4.25E-6||SGD DESC:Putative protein of unknown function Gene:YML009C-A(YML009C-A_d)|FD-Score:3.75|P-value:8.89E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML047W-A(YML047W-A_d)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL193W(YNL193W_p)|FD-Score:3.23|P-value:6.27E-4||SGD DESC:Putative protein of unknown function; exhibits a two-hybrid interaction with Yhr151cp in a large-scale analysis Gene:YNL285W(YNL285W_d)|FD-Score:5.44|P-value:2.62E-8||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR170W(YOR170W_d)|FD-Score:3.83|P-value:6.38E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene LCB4 Gene:YOR228C(YOR228C)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YPS3(YLR121C)|FD-Score:-3.98|P-value:3.44E-5||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR170C4.701.33E-60.38SEC7Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles
YJR042W4.327.83E-60.31NUP85Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75
YLR088W4.003.10E-50.19GAA1Subunit of the GPI (glycosylphosphatidylinositol):protein transamidase complex, removes the GPI-anchoring signal and attaches GPI to proteins in the ER
YNL308C3.826.78E-50.03KRI1Essential nucleolar protein required for 40S ribosome biogenesis; associate with snR30; physically and functionally interacts with Krr1p
YFL039C3.787.71E-50.03ACT1Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions
YBR002C3.758.76E-50.01RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YGR251W3.749.03E-50.02NOP19Ribosome biogenesis factor; nucleolar protein associated with pre-rRNA components of the 90S preribosome, required for cleavage of pre-rRNA at A0, A1 and A2 sites; interacts with RNA helicase Dhr2p and RNA helicase-like protein Utp25p; required for incorporation of Utp25p into preribosomes
YMR277W3.739.68E-50.16FCP1Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p)
YJR072C3.571.79E-40.20NPA3Member of the conserved GPN-loop GTPase family; has a role in transport of RNA polymerase II to the nucleus; exhibits GTP-dependent binding to PolII; has ATPase activity; involved in sister chromatid cohesion; phosphorylated by the Pcl1p-Pho85p kinase complex; human homolog XAB1 interacts with human RNA polymerase II; protein abundance increases in response to DNA replication stress
YOR168W3.363.84E-40.20GLN4Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions
YNL088W3.167.82E-40.01TOP2Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation
YOR075W3.158.17E-40.00UFE1t-SNARE required for retrograde vesicular traffic and homotypic ER membrane fusion; forms a complex with the SNAREs Sec22p, Sec20p and Use1p to mediate fusion of Golgi-derived vesicles at the ER
YLR076C_d3.158.23E-47.41E-4YLR076C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10
YBL014C3.158.25E-40.03RRN6Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
YIL061C3.119.21E-40.04SNP1Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR319C6.144.22E-10FET4Low-affinity Fe(II) transporter of the plasma membrane
YNL285W_d5.442.62E-8YNL285W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YFR018C_p5.042.39E-7YFR018C_pPutative protein of unknown function
YER097W_d5.032.42E-7YER097W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YOR120W4.798.46E-7GCY1Glycerol dehydrogenase;involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication
YLR131C4.779.34E-7ACE2Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication
YLR412C-A_p4.454.25E-6YLR412C-A_pPutative protein of unknown function
YNR030W4.003.16E-5ALG12Alpha-1,6-mannosyltransferase localized to the ER; responsible for the addition of the alpha-1,6 mannose to dolichol-linked Man7GlcNAc2, acts in the dolichol pathway for N-glycosylation
YJR111C_p3.963.68E-5YJR111C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria
YGL072C_d3.953.83E-5YGL072C_dDubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2
YER169W3.944.09E-5RPH1JmjC domain-containing histone demethylase; specifically demethylates H3K36 tri- and dimethyl modification states; associates with actively transcribed (RNA polymerase II) regions in vivo and specifically targets H3K36 in its trimethylation state as its substrate; transcriptional repressor of PHR1; Rph1p phosphorylation during DNA damage is under control of the MEC1-RAD53 pathway; RPH1 has a paralog, GIS1, that arose from the whole genome duplication
YML026C3.885.32E-5RPS18BProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18B has a paralog, RPS18A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
YOR170W_d3.836.38E-5YOR170W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene LCB4
YER135C_d3.836.45E-5YER135C_dDubious open reading frame unlikely to encode a protein; YER135C is not an essential gene
YGL084C3.768.56E-5GUP1Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport

GO enrichment analysis for SGTC_2657
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1023.42E-15SGTC_999SEC7.066 22.7 μMChemDiv (Drug-like library)7405110.0555556Golgi
0.0939.30E-13SGTC_32599138485 49.5 μMChembridge (Drug-like library)176461530.0366972
0.0815.34E-10SGTC_22426630449 61.3 μMChembridge (Fragment library)6912490.0588235Golgi
0.0799.67E-10SGTC_2674ligustilide 100.0 μMMicrosource (Natural product library)53190220.0882353
0.0732.34E-8SGTC_1505k297-0037 20.6 μMChemDiv (Drug-like library)40893960.0178571
0.0708.91E-8SGTC_14023909-7673 35.9 μMChemDiv (Drug-like library)53280140.0185185
0.0691.08E-7SGTC_9721319-0219 195.0 μMChemDiv (Drug-like library)239037020.0708661
0.0681.76E-7SGTC_316k060-0003 58.6 μMChemDiv (Drug-like library)61523140.017094
0.0672.33E-7SGTC_22277312221 64.1 μMChembridge (Fragment library)8871880.0471698
0.0673.09E-7SGTC_14414106-0040 13.0 μMChemDiv (Drug-like library)29041800.0350877
0.0655.07E-7SGTC_12500485-0349 40.2 μMChemDiv (Drug-like library)68427940.0571429
0.0655.89E-7SGTC_2676harmalol 100.0 μMMicrosource (Natural product library)54591870.0471698mitochondrial processes
0.0656.56E-7SGTC_2712st079126 47.7 μMTimTec (Natural product derivative library)31529700.0756303
0.0612.70E-6SGTC_1603453-0671 289.7 μMChemDiv (Drug-like library)53222680.064
0.0603.29E-6SGTC_30919116372 49.5 μMChembridge (Drug-like library)171697930.0642202

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_277nystatin15.5 μM111468707Miscellaneous926.0949-2.6041218
SGTC_1872amphotericin b148 nM0.67021316667679Microsource (Natural product library)924.07902-2.6171218RNA pol III & RNase P/MRP
SGTC_2663amphotericin b100 μM0.67021316667679Microsource (Natural product library)924.07902-2.6171218
SGTC_1574spiramycin23.7 μM0.2246386419898TimTec (Pure natural product library)843.052662.049416
SGTC_1578josamycin24.2 μM0.2137935282165TimTec (Pure natural product library)827.994962.957316
SGTC_2665lovastatin100 μM0.21238953232Miscellaneous404.539644.21815
SGTC_504lovastatin124 μM0.21238953232ICCB bioactive library404.539644.21815
SGTC_1545lovastatin49.4 μM0.21238912923612TimTec (Pure natural product library)404.539644.21815
SGTC_250brefeldin a552.24 μM0.1919196436187ICCB bioactive library280.359362.15224PDR1
SGTC_1079idarubicin2.36 μM0.189655636362NIH Clinical Collection533.954821.026610DNA damage response
SGTC_257daunorubicin18.5 μM0.17741930323Miscellaneous527.519860.628511anthracycline transcription coupled DNA repair
SGTC_2662daunorubicin22.37 μM0.17741930323Miscellaneous527.519860.628511anthracycline transcription coupled DNA repair
SGTC_2746daunorubicin9.88 μM0.17741930323Miscellaneous527.519860.628511anthracycline transcription coupled DNA repair
SGTC_272doxorubicin8.13 μM0.17322831703Miscellaneous543.51926-0.044612anthracycline transcription coupled DNA repair
SGTC_2747doxorubicin7.63 μM0.17322831703Miscellaneous543.51926-0.044612anthracycline transcription coupled DNA repair
SGTC_1085doxorubicin7.67 μM0.173228443939NIH Clinical Collection579.98020.338712anthracycline transcription coupled DNA repair
SGTC_1105epirubicin18.1 μM0.17322865348NIH Clinical Collection579.98020.338712anthracycline transcription coupled DNA repair