dactinomycin FDA approved compound

bis[1-[10,13-dimethyl-2,5,8,11,14-pentaoxo-3,9-di(propan-2-yl)-1,4,7,10,13-pentazabicyclo[13.3.0]octadecan-6-yl]ethyl] 2-amino-4,6-dimethyl-3-oxophenoxazine-1,9-dicarboxylate

An Actinomycin D Classic transcription inhibitor.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2661
Screen concentration 13.0 μM
Source Microsource (Natural product library)
PubChem CID 45006140
SMILES CC1=C2C(=C(C=C1)C(=O)OC(C)C3C(=O)NC(C(=O)N4CCCC4C(=O)N(CC(=O)N(C(C(=O)N3)C(C)C)C)C)C(C)C)N=C5C(=C(C(=O)C(=C5O2)C)N)C(=O)OC(C)C6C(=O)NC(C(=O)N7CCCC7C(=O)N(CC(=O)N(C(C(=O)N6)C(C)C)C)C)C(C)C
Standardized SMILES CC(C)C1NC(=O)C(NC(=O)C(C(C)C)N(C)C(=O)CN(C)C(=O)C2CCCN2C1=O)C(C)OC(=O)C3=C(N)C(=O)C(=C4Oc5c(C)ccc(C(=O)OC(C)C6NC(=O)C(C(C)C)N(C)C(=O)CN(C)C(=O)C7CCCN7C(=O)C(NC6=O)C(C)C)c5N=C34)C
Molecular weight 1255.417
ALogP 0.36
H-bond donor count 5
H-bond acceptor count 18
Response signature redox potentiating

Pool Growth Kinetics
% growth inhibition (Het. pool) 0
% growth inhibition (Hom. pool) 1.26


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 45006140
Download HIP data (tab-delimited text)  (excel)
Gene:CIA1(YDR267C)|FD-Score:3.09|P-value:9.86E-4|Clearance:0.06||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia2p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; contains WD40 repeats; ortholog of human Ciao1 protein Gene:ERG11(YHR007C)|FD-Score:3.67|P-value:1.21E-4|Clearance:0.46||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ESP1(YGR098C)|FD-Score:-3.7|P-value:1.07E-4|Clearance:0||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:PRI2(YKL045W)|FD-Score:3.69|P-value:1.10E-4|Clearance:0.02||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:RLP24(YLR009W)|FD-Score:3.22|P-value:6.51E-4|Clearance:0.04||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:SAM35(YHR083W)|FD-Score:3.17|P-value:7.59E-4|Clearance:0.08||SGD DESC:Essential component of the sorting and assembly machinery (SAM complex or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane Gene:TRS31(YDR472W)|FD-Score:4.43|P-value:4.65E-6|Clearance:0.74||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:CIA1(YDR267C)|FD-Score:3.09|P-value:9.86E-4|Clearance:0.06||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia2p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; contains WD40 repeats; ortholog of human Ciao1 protein Gene:ERG11(YHR007C)|FD-Score:3.67|P-value:1.21E-4|Clearance:0.46||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ESP1(YGR098C)|FD-Score:-3.7|P-value:1.07E-4|Clearance:0||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:PRI2(YKL045W)|FD-Score:3.69|P-value:1.10E-4|Clearance:0.02||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:RLP24(YLR009W)|FD-Score:3.22|P-value:6.51E-4|Clearance:0.04||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:SAM35(YHR083W)|FD-Score:3.17|P-value:7.59E-4|Clearance:0.08||SGD DESC:Essential component of the sorting and assembly machinery (SAM complex or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane Gene:TRS31(YDR472W)|FD-Score:4.43|P-value:4.65E-6|Clearance:0.74||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 45006140
Download HOP data (tab-delimited text)  (excel)
Gene:ABF2(YMR072W)|FD-Score:3.83|P-value:6.47E-5||SGD DESC:Mitochondrial DNA-binding protein involved in mitochondrial DNA replication and recombination, member of HMG1 DNA-binding protein family; activity may be regulated by protein kinase A phosphorylation Gene:ADY2(YCR010C)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Acetate transporter required for normal sporulation; phosphorylated in mitochondria; ADY2 has a paralog, ATO2, that arose from the whole genome duplication Gene:ALD6(YPL061W)|FD-Score:4.43|P-value:4.79E-6||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:AMN1(YBR158W)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Protein required for daughter cell separation, multiple mitotic checkpoints, and chromosome stability; contains 12 degenerate leucine-rich repeat motifs; expression is induced by the Mitotic Exit Network (MEN) Gene:ARF1(YDL192W)|FD-Score:6.76|P-value:6.72E-12||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication Gene:ARR2(YPR200C)|FD-Score:4.06|P-value:2.46E-5||SGD DESC:Arsenate reductase required for arsenate resistance; converts arsenate to arsenite which can then be exported from cells by Arr3p Gene:BUD16(YEL029C)|FD-Score:3.17|P-value:7.62E-4||SGD DESC:Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) Gene:CBC2(YPL178W)|FD-Score:4.03|P-value:2.77E-5||SGD DESC:Small subunit of the heterodimeric cap binding complex that also contains Sto1p, component of the spliceosomal commitment complex; interacts with Npl3p, possibly to package mRNA for export from the nucleus; contains an RNA-binding motif Gene:DUG1(YFR044C)|FD-Score:3.85|P-value:5.96E-5||SGD DESC:Cys-Gly metallo-di-peptidase; forms a complex with Dug2p and Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:DYN2(YDR424C)|FD-Score:3.12|P-value:9.16E-4||SGD DESC:Cytoplasmic light chain dynein, microtubule motor protein; proposed to be involved in the assembly of the nuclear pore complex Gene:ECM5(YMR176W)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Non-essential protein of unknown function; contains ATP/GTP-binding site motif A; null mutant exhibits cellular volume up to four times greater than wild-type, also large drooping buds with elongated necks; relative distribution to the nucleus increases upon DNA replication stress Gene:FAR8(YMR029C)|FD-Score:-3.23|P-value:6.20E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p Gene:FLO1(YAR050W)|FD-Score:-3.22|P-value:6.39E-4||SGD DESC:Lectin-like protein involved in flocculation, cell wall protein that binds to mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin sensitive and heat resistant; similar to Flo5p Gene:FLR1(YBR008C)|FD-Score:-3.4|P-value:3.32E-4||SGD DESC:Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; involved in efflux of fluconazole, diazaborine, benomyl, methotrexate, and other drugs; expression induced in cells treated with the mycotoxin patulin; relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:FYV7(YLR068W)|FD-Score:4.63|P-value:1.81E-6||SGD DESC:Essential protein required for maturation of 18S rRNA; required for survival upon exposure to K1 killer toxin Gene:HAM1(YJR069C)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Nucleoside triphosphate pyrophosphohydrolase; active against a wide range of substrates including ITP, dITP and XTP; mediates exclusion of noncanonical purines and pyrimadines from deoxyribonucleoside triphosphate pools; mutant is sensitive to the base analog 6-N-hydroxylaminopurine; protein abundance increases in response to DNA replication stress Gene:JEN1(YKL217W)|FD-Score:3.32|P-value:4.58E-4||SGD DESC:Monocarboxylate/proton symporter of the plasma membrane; transport activity is dependent on the pH gradient across the membrane; mediates high-affinity uptake of carbon sources lactate, pyuvate, and acetate, and also of the micronutrient selenite, whose structure mimics that of monocarboxylates; expression and localization are tightly regulated, with transcription repression, mRNA degradation, and protein endocytosis and degradation all occurring in the presence of glucose Gene:KAR9(YPL269W)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase Gene:KIP2(YPL155C)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:Kinesin-related motor protein involved in mitotic spindle positioning, stabilizes microtubules by targeting Bik1p to the plus end; Kip2p levels are controlled during the cell cycle Gene:LEM3(YNL323W)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:MDM20(YOL076W)|FD-Score:3.24|P-value:6.05E-4||SGD DESC:Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly Gene:MKK2(YPL140C)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk1p; MKK2 has a paralog, MKK1, that arose from the whole genome duplication Gene:MRF1(YGL143C)|FD-Score:3.1|P-value:9.76E-4||SGD DESC:Mitochondrial translation release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability Gene:MTQ1(YNL063W)|FD-Score:-3.14|P-value:8.58E-4||SGD DESC:S-adenosylmethionine-dependent methyltransferase; methylates translational release factor Mrf1p; similar to E.coli PrmC; is not an essential gene Gene:NUM1(YDR150W)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:NUP53(YMR153W)|FD-Score:-3.27|P-value:5.29E-4||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport; involved in regulation of transcription and mitosis; induces membrane tubulation, which may contribute to nuclear pore assembly; NUP53 has a paralog, ASM4, that arose from the whole genome duplication Gene:PAF1(YBR279W)|FD-Score:3.99|P-value:3.37E-5||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 Gene:PEP12(YOR036W)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PEP3(YLR148W)|FD-Score:4.01|P-value:3.03E-5||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PEP7(YDR323C)|FD-Score:4.63|P-value:1.86E-6||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PET112(YBL080C)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog Gene:PET20(YPL159C)|FD-Score:4.54|P-value:2.76E-6||SGD DESC:Mitochondrial protein, required for respiratory growth under some conditions and for stability of the mitochondrial genome Gene:PUT2(YHR037W)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:Delta-1-pyrroline-5-carboxylate dehydrogenase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of the human homolog causes HPII, an autosomal recessive inborn error of metabolism Gene:RAX1(YOR301W)|FD-Score:-3.91|P-value:4.70E-5||SGD DESC:Protein involved in bud site selection during bipolar budding; localization requires Rax2p; has similarity to members of the insulin-related peptide superfamily Gene:RGP1(YDR137W)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RPA49(YNL248C)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:RNA polymerase I subunit A49 Gene:RPN10(YHR200W)|FD-Score:3.32|P-value:4.51E-4||SGD DESC:Non-ATPase base subunit of the 19S regulatory particle (RP) of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the RP; binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein Gene:RPP1B(YDL130W)|FD-Score:3.28|P-value:5.23E-4||SGD DESC:Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component Gene:RSM23(YGL129C)|FD-Score:3.74|P-value:9.09E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to mammalian apoptosis mediator proteins; null mutation prevents induction of apoptosis by overproduction of metacaspase Mca1p Gene:SKG1(YKR100C)|FD-Score:3.76|P-value:8.53E-5||SGD DESC:Transmembrane protein with a role in cell wall polymer composition; localizes on the inner surface of the plasma membrane at the bud and in the daughter cell Gene:SNF12(YNR023W)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive Gene:TPN1(YGL186C)|FD-Score:4.05|P-value:2.55E-5||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRX1(YLR043C)|FD-Score:3.74|P-value:9.04E-5||SGD DESC:Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p, required for ER-Golgi transport and vacuole inheritance; protein abundance increases in response to DNA replication stress Gene:VMA1(YDL185W)|FD-Score:3.15|P-value:8.17E-4||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA13(YPR036W)|FD-Score:4.96|P-value:3.54E-7||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VMA16(YHR026W)|FD-Score:4.53|P-value:3.02E-6||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:VMA7(YGR020C)|FD-Score:3.75|P-value:9.00E-5||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VMA9(YCL005W-A)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis Gene:VPS1(YKR001C)|FD-Score:3.8|P-value:7.13E-5||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS20(YMR077C)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:VPS21(YOR089C)|FD-Score:4.4|P-value:5.30E-6||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS25(YJR102C)|FD-Score:5.14|P-value:1.37E-7||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS3(YDR495C)|FD-Score:5.61|P-value:1.02E-8||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS36(YLR417W)|FD-Score:3.72|P-value:9.89E-5||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:VPS63(YLR261C_d)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YBR090C(YBR090C_p)|FD-Score:-3.83|P-value:6.53E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:YBR174C(YBR174C_d)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective Gene:YDL068W(YDL068W_d)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL108C(YGL108C_p)|FD-Score:3.11|P-value:9.28E-4||SGD DESC:Protein of unknown function, predicted to be palmitoylated; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress Gene:YKL097C(YKL097C_d)|FD-Score:3.15|P-value:8.21E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species Gene:YLR282C(YLR282C_d)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition Gene:YOR139C(YOR139C_d)|FD-Score:4.83|P-value:6.68E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W Gene:YPR197C(YPR197C_d)|FD-Score:4.42|P-value:4.92E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPT6(YLR262C)|FD-Score:5.67|P-value:7.13E-9||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:YRR1(YOR162C)|FD-Score:5.56|P-value:1.35E-8||SGD DESC:Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication Gene:ZRC1(YMR243C)|FD-Score:3.14|P-value:8.37E-4||SGD DESC:Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication Gene:ABF2(YMR072W)|FD-Score:3.83|P-value:6.47E-5||SGD DESC:Mitochondrial DNA-binding protein involved in mitochondrial DNA replication and recombination, member of HMG1 DNA-binding protein family; activity may be regulated by protein kinase A phosphorylation Gene:ADY2(YCR010C)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Acetate transporter required for normal sporulation; phosphorylated in mitochondria; ADY2 has a paralog, ATO2, that arose from the whole genome duplication Gene:ALD6(YPL061W)|FD-Score:4.43|P-value:4.79E-6||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:AMN1(YBR158W)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Protein required for daughter cell separation, multiple mitotic checkpoints, and chromosome stability; contains 12 degenerate leucine-rich repeat motifs; expression is induced by the Mitotic Exit Network (MEN) Gene:ARF1(YDL192W)|FD-Score:6.76|P-value:6.72E-12||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication Gene:ARR2(YPR200C)|FD-Score:4.06|P-value:2.46E-5||SGD DESC:Arsenate reductase required for arsenate resistance; converts arsenate to arsenite which can then be exported from cells by Arr3p Gene:BUD16(YEL029C)|FD-Score:3.17|P-value:7.62E-4||SGD DESC:Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) Gene:CBC2(YPL178W)|FD-Score:4.03|P-value:2.77E-5||SGD DESC:Small subunit of the heterodimeric cap binding complex that also contains Sto1p, component of the spliceosomal commitment complex; interacts with Npl3p, possibly to package mRNA for export from the nucleus; contains an RNA-binding motif Gene:DUG1(YFR044C)|FD-Score:3.85|P-value:5.96E-5||SGD DESC:Cys-Gly metallo-di-peptidase; forms a complex with Dug2p and Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:DYN2(YDR424C)|FD-Score:3.12|P-value:9.16E-4||SGD DESC:Cytoplasmic light chain dynein, microtubule motor protein; proposed to be involved in the assembly of the nuclear pore complex Gene:ECM5(YMR176W)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Non-essential protein of unknown function; contains ATP/GTP-binding site motif A; null mutant exhibits cellular volume up to four times greater than wild-type, also large drooping buds with elongated necks; relative distribution to the nucleus increases upon DNA replication stress Gene:FAR8(YMR029C)|FD-Score:-3.23|P-value:6.20E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p Gene:FLO1(YAR050W)|FD-Score:-3.22|P-value:6.39E-4||SGD DESC:Lectin-like protein involved in flocculation, cell wall protein that binds to mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin sensitive and heat resistant; similar to Flo5p Gene:FLR1(YBR008C)|FD-Score:-3.4|P-value:3.32E-4||SGD DESC:Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; involved in efflux of fluconazole, diazaborine, benomyl, methotrexate, and other drugs; expression induced in cells treated with the mycotoxin patulin; relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:FYV7(YLR068W)|FD-Score:4.63|P-value:1.81E-6||SGD DESC:Essential protein required for maturation of 18S rRNA; required for survival upon exposure to K1 killer toxin Gene:HAM1(YJR069C)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Nucleoside triphosphate pyrophosphohydrolase; active against a wide range of substrates including ITP, dITP and XTP; mediates exclusion of noncanonical purines and pyrimadines from deoxyribonucleoside triphosphate pools; mutant is sensitive to the base analog 6-N-hydroxylaminopurine; protein abundance increases in response to DNA replication stress Gene:JEN1(YKL217W)|FD-Score:3.32|P-value:4.58E-4||SGD DESC:Monocarboxylate/proton symporter of the plasma membrane; transport activity is dependent on the pH gradient across the membrane; mediates high-affinity uptake of carbon sources lactate, pyuvate, and acetate, and also of the micronutrient selenite, whose structure mimics that of monocarboxylates; expression and localization are tightly regulated, with transcription repression, mRNA degradation, and protein endocytosis and degradation all occurring in the presence of glucose Gene:KAR9(YPL269W)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase Gene:KIP2(YPL155C)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:Kinesin-related motor protein involved in mitotic spindle positioning, stabilizes microtubules by targeting Bik1p to the plus end; Kip2p levels are controlled during the cell cycle Gene:LEM3(YNL323W)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:MDM20(YOL076W)|FD-Score:3.24|P-value:6.05E-4||SGD DESC:Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly Gene:MKK2(YPL140C)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk1p; MKK2 has a paralog, MKK1, that arose from the whole genome duplication Gene:MRF1(YGL143C)|FD-Score:3.1|P-value:9.76E-4||SGD DESC:Mitochondrial translation release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability Gene:MTQ1(YNL063W)|FD-Score:-3.14|P-value:8.58E-4||SGD DESC:S-adenosylmethionine-dependent methyltransferase; methylates translational release factor Mrf1p; similar to E.coli PrmC; is not an essential gene Gene:NUM1(YDR150W)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:NUP53(YMR153W)|FD-Score:-3.27|P-value:5.29E-4||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport; involved in regulation of transcription and mitosis; induces membrane tubulation, which may contribute to nuclear pore assembly; NUP53 has a paralog, ASM4, that arose from the whole genome duplication Gene:PAF1(YBR279W)|FD-Score:3.99|P-value:3.37E-5||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 Gene:PEP12(YOR036W)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PEP3(YLR148W)|FD-Score:4.01|P-value:3.03E-5||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PEP7(YDR323C)|FD-Score:4.63|P-value:1.86E-6||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PET112(YBL080C)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog Gene:PET20(YPL159C)|FD-Score:4.54|P-value:2.76E-6||SGD DESC:Mitochondrial protein, required for respiratory growth under some conditions and for stability of the mitochondrial genome Gene:PUT2(YHR037W)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:Delta-1-pyrroline-5-carboxylate dehydrogenase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of the human homolog causes HPII, an autosomal recessive inborn error of metabolism Gene:RAX1(YOR301W)|FD-Score:-3.91|P-value:4.70E-5||SGD DESC:Protein involved in bud site selection during bipolar budding; localization requires Rax2p; has similarity to members of the insulin-related peptide superfamily Gene:RGP1(YDR137W)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RPA49(YNL248C)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:RNA polymerase I subunit A49 Gene:RPN10(YHR200W)|FD-Score:3.32|P-value:4.51E-4||SGD DESC:Non-ATPase base subunit of the 19S regulatory particle (RP) of the 26S proteasome; N-terminus plays a role in maintaining the structural integrity of the RP; binds selectively to polyubiquitin chains; homolog of the mammalian S5a protein Gene:RPP1B(YDL130W)|FD-Score:3.28|P-value:5.23E-4||SGD DESC:Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component Gene:RSM23(YGL129C)|FD-Score:3.74|P-value:9.09E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to mammalian apoptosis mediator proteins; null mutation prevents induction of apoptosis by overproduction of metacaspase Mca1p Gene:SKG1(YKR100C)|FD-Score:3.76|P-value:8.53E-5||SGD DESC:Transmembrane protein with a role in cell wall polymer composition; localizes on the inner surface of the plasma membrane at the bud and in the daughter cell Gene:SNF12(YNR023W)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive Gene:TPN1(YGL186C)|FD-Score:4.05|P-value:2.55E-5||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRX1(YLR043C)|FD-Score:3.74|P-value:9.04E-5||SGD DESC:Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system which protects cells against oxidative and reductive stress; forms LMA1 complex with Pbi2p; acts as a cofactor for Tsa1p, required for ER-Golgi transport and vacuole inheritance; protein abundance increases in response to DNA replication stress Gene:VMA1(YDL185W)|FD-Score:3.15|P-value:8.17E-4||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA13(YPR036W)|FD-Score:4.96|P-value:3.54E-7||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VMA16(YHR026W)|FD-Score:4.53|P-value:3.02E-6||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:VMA7(YGR020C)|FD-Score:3.75|P-value:9.00E-5||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VMA9(YCL005W-A)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis Gene:VPS1(YKR001C)|FD-Score:3.8|P-value:7.13E-5||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS20(YMR077C)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:VPS21(YOR089C)|FD-Score:4.4|P-value:5.30E-6||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS25(YJR102C)|FD-Score:5.14|P-value:1.37E-7||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS3(YDR495C)|FD-Score:5.61|P-value:1.02E-8||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS36(YLR417W)|FD-Score:3.72|P-value:9.89E-5||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:VPS63(YLR261C_d)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YBR090C(YBR090C_p)|FD-Score:-3.83|P-value:6.53E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:YBR174C(YBR174C_d)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective Gene:YDL068W(YDL068W_d)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL108C(YGL108C_p)|FD-Score:3.11|P-value:9.28E-4||SGD DESC:Protein of unknown function, predicted to be palmitoylated; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress Gene:YKL097C(YKL097C_d)|FD-Score:3.15|P-value:8.21E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species Gene:YLR282C(YLR282C_d)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition Gene:YOR139C(YOR139C_d)|FD-Score:4.83|P-value:6.68E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W Gene:YPR197C(YPR197C_d)|FD-Score:4.42|P-value:4.92E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPT6(YLR262C)|FD-Score:5.67|P-value:7.13E-9||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:YRR1(YOR162C)|FD-Score:5.56|P-value:1.35E-8||SGD DESC:Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication Gene:ZRC1(YMR243C)|FD-Score:3.14|P-value:8.37E-4||SGD DESC:Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR472W4.434.65E-60.74TRS31One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic
YKL045W3.691.10E-40.02PRI2Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair
YHR007C3.671.21E-40.46ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YLR009W3.226.51E-40.04RLP24Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp
YHR083W3.177.59E-40.08SAM35Essential component of the sorting and assembly machinery (SAM complex or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane
YDR267C3.099.86E-40.06CIA1Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia2p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; contains WD40 repeats; ortholog of human Ciao1 protein
YLL018C3.040.001190.00DPS1Aspartyl-tRNA synthetase, primarily cytoplasmic; homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation
YER146W3.040.001200.18LSM5Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA
YJL194W2.860.002140.02CDC6Essential ATP-binding protein required for DNA replication; component of the pre-replicative complex (pre-RC) which requires ORC to associate with chromatin and is in turn required for Mcm2-7p DNA association; homologous to S. pombe Cdc18p; relocalizes from nucleus to cytoplasm upon DNA replication stress
YBL105C2.830.002300.03PKC1Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
YLR145W2.800.002520.01RMP1Subunit of RNase MRP, which processes pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; unlike most subunits, not shared between RNase MRP and nuclear RNase P
YBR190W_d2.800.002590.05YBR190W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W
YBR237W2.740.003060.06PRP5RNA helicase in the DEAD-box family, necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA
YLR005W2.680.003690.03SSL1Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p
YNL272C2.650.004030.01SEC2Guanyl-nucleotide exchange factor for the small G-protein Sec4p; essential for post-Golgi vesicle transport and for autophagy; associates with the exocyst, via exocyst subunit Sec15p, on secretory vesicles

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDL192W6.766.72E-12ARF1ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication
YLR262C5.677.13E-9YPT6Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6
YDR495C5.611.02E-8VPS3Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase
YOR162C5.561.35E-8YRR1Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication
YJR102C5.141.37E-7VPS25Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome
YPR036W4.963.54E-7VMA13Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase
YOR139C_d4.836.68E-7YOR139C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W
YLR068W4.631.81E-6FYV7Essential protein required for maturation of 18S rRNA; required for survival upon exposure to K1 killer toxin
YDR323C4.631.86E-6PEP7Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance
YPL159C4.542.76E-6PET20Mitochondrial protein, required for respiratory growth under some conditions and for stability of the mitochondrial genome
YHR026W4.533.02E-6VMA16Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain
YPL061W4.434.79E-6ALD6Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress
YPR197C_d4.424.92E-6YPR197C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YOR089C4.405.30E-6VPS21Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication
YMR077C4.161.61E-5VPS20Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes

GO enrichment analysis for SGTC_2661
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1882.66E-48SGTC_748act1.228 77.9 μMChemDiv (Drug-like library)44215050.0183486redox potentiating
0.1871.41E-47SGTC_8410251-0215 91.2 μMChemDiv (Drug-like library)38886140.038835
0.1734.57E-41SGTC_1593justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one 54.9 μMTimTec (Pure natural product library)4428820.0731707redox potentiating
0.1603.70E-35SGTC_14954469-0029 237.0 μMChemDiv (Drug-like library)7936800.0495868
0.1579.82E-34SGTC_2567aureobasidin a 60.0 nMMiscellaneous99634300.246377
0.1476.92E-30SGTC_391gliotoxin 8.3 μMMiscellaneous62230.0708661
0.1479.61E-30SGTC_459nigericin 15.3 μMICCB bioactive library342300.0696203
0.1432.08E-28SGTC_1687st020778 55.4 μMTimTec (Natural product derivative library)42592990.078125
0.1432.67E-28SGTC_6704204-0025 119.0 μMChemDiv (Drug-like library)7190390.0940171copper-dependent oxidative stress
0.1398.16E-27SGTC_1873rapamycin 2.0 nMMiscellaneous52846160.117647
0.1374.66E-26SGTC_1240136-0217 7.1 μMChemDiv (Drug-like library)39769050.0714286mitochondrial stress
0.1271.18E-22SGTC_1660st012960 28.1 μMTimTec (Natural product derivative library)242072660.171171iron homeostasis
0.1262.32E-22SGTC_2615purpurogallin-4-carboxylic acid 100.0 μMMicrosource (Natural product library)2693150.0625redox potentiating
0.1232.20E-21SGTC_102gallobenzophenone 83.6 μMChemDiv (Drug-like library)708370.0761905redox potentiating
0.1223.79E-21SGTC_2737bleomycin 30.5 nMMiscellaneous162113180.0694444Golgi

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_2420aureobasidin a50 nM0.2463779963430Miscellaneous1101.419486.007411
SGTC_2567aureobasidin a60 nM0.2463779963430Miscellaneous1101.419486.007411
SGTC_1639Lupinine o-methylbenzoicacid ester hydrochloride61.8 μM0.17117117037758TimTec (Natural product derivative library)323.857544.19413ergosterol biosynthesis
SGTC_1660st01296028.1 μM0.17117124207266TimTec (Natural product derivative library)580.4471550.052815iron homeostasis
SGTC_351caspofungin25 nM0.1575769963413Miscellaneous1093.31312-2.891618cell wall
SGTC_1986st07181245 μM0.15211838594TimTec (Natural product derivative library)426.247382.35827
SGTC_10244507-039526 μM0.1512614201836ChemDiv (Drug-like library)349.3568030.67916TSC3-RPN4
SGTC_1983st07181346.9 μM0.15079411838595TimTec (Natural product derivative library)426.247382.35827RPP1 & pyrimidine depletion
SGTC_1981st07182647 μM0.14960611838600TimTec (Natural product derivative library)425.355231.971210
SGTC_15034585-001261.3 μM0.148762922052ChemDiv (Drug-like library)338.464943.81214
SGTC_1656st01292111 μM0.1481486732821TimTec (Natural product derivative library)375.14928-0.315