theaflavin digallate

[(2R,3R)-2-[1-[(2R,3R)-5,7-dihydroxy-3-(3,4,5-trihydroxybenzoyl)oxy-3,4-dihydro-2H-chromen-2-yl]-3,4,5-trihydroxy-6-oxobenzo[7]annulen-8-yl]-5,7-dihydroxy-3,4-dihydro-2H-chromen-3-yl] 3,4,5-trihydroxybenzoate

Theaflavin digallate is an antioxidant natural phenol found in black tea, and a theaflavin derivative.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2675
Screen concentration 100.0 μM
Source Microsource (Natural product library)
PubChem CID 467320
SMILES C1C(C(OC2=CC(=CC(=C21)O)O)C3=CC(=O)C(=C4C(=C3)C(=CC(=C4O)O)C5C(CC6=C(C=C(C=C6O5)O)O)OC(=O)C7=CC(=C(C(=C7)O)O)O)O)OC(=O)C8=CC(=C(C(=C8)O)O)O
Standardized SMILES OC1=CC(=Cc2c(cc(O)c(O)c2C1=O)C3Oc4cc(O)cc(O)c4CC3OC(=O)c5cc(O)c(O)c(O)c5)C6Oc7cc(O)cc(O)c7CC6OC(=O)c8cc(O)c(O)c(O)c8
Molecular weight 868.7022
ALogP 4.12
H-bond donor count 13
H-bond acceptor count 20
Response signature iron homeostasis

Pool Growth Kinetics
% growth inhibition (Het. pool) 22.08
% growth inhibition (Hom. pool) 15.43


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 467320
Download HIP data (tab-delimited text)  (excel)
Gene:ARP7(YPR034W)|FD-Score:-4.12|P-value:1.91E-5|Clearance:0||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:ARP9(YMR033W)|FD-Score:-4.46|P-value:4.01E-6|Clearance:0||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CBK1(YNL161W)|FD-Score:3.41|P-value:3.23E-4|Clearance:0.08||SGD DESC:Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress Gene:CDC31(YOR257W)|FD-Score:3.87|P-value:5.47E-5|Clearance:0.16||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:CIA1(YDR267C)|FD-Score:3.3|P-value:4.80E-4|Clearance:0.18||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia2p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; contains WD40 repeats; ortholog of human Ciao1 protein Gene:LSM2(YBL026W)|FD-Score:3.46|P-value:2.72E-4|Clearance:0.02||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MCD4(YKL165C)|FD-Score:3.67|P-value:1.20E-4|Clearance:0.22||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:NOP58(YOR310C)|FD-Score:4.58|P-value:2.32E-6|Clearance:0.02||SGD DESC:Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:NUS1(YDL193W)|FD-Score:-3.49|P-value:2.42E-4|Clearance:0||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PMI40(YER003C)|FD-Score:3.44|P-value:2.89E-4|Clearance:0.03||SGD DESC:Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation Gene:RFC1(YOR217W)|FD-Score:3.71|P-value:1.03E-4|Clearance:0.04||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RPC53(YDL150W)|FD-Score:-3.11|P-value:9.43E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C53 Gene:RPL25(YOL127W)|FD-Score:5.75|P-value:4.45E-9|Clearance:0.95||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPL32(YBL092W)|FD-Score:4.28|P-value:9.33E-6|Clearance:0.03||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:SGV1(YPR161C)|FD-Score:3.33|P-value:4.27E-4|Clearance:0.02||SGD DESC:Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins Gene:SSS1(YDR086C)|FD-Score:4.25|P-value:1.07E-5|Clearance:0.38||SGD DESC:Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p Gene:TFA2(YKR062W)|FD-Score:3.12|P-value:9.00E-4|Clearance:0.1||SGD DESC:TFIIE small subunit, involved in RNA polymerase II transcription initiation Gene:TIM12(YBR091C)|FD-Score:3.32|P-value:4.53E-4|Clearance:0.02||SGD DESC:Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins Gene:TOM40(YMR203W)|FD-Score:4.37|P-value:6.28E-6|Clearance:0.09||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:TRR1(YDR353W)|FD-Score:4.56|P-value:2.52E-6|Clearance:0.2||SGD DESC:Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication Gene:YDL196W(YDL196W_d)|FD-Score:5.53|P-value:1.64E-8|Clearance:0.95||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:ARP7(YPR034W)|FD-Score:-4.12|P-value:1.91E-5|Clearance:0||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:ARP9(YMR033W)|FD-Score:-4.46|P-value:4.01E-6|Clearance:0||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CBK1(YNL161W)|FD-Score:3.41|P-value:3.23E-4|Clearance:0.08||SGD DESC:Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress Gene:CDC31(YOR257W)|FD-Score:3.87|P-value:5.47E-5|Clearance:0.16||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:CIA1(YDR267C)|FD-Score:3.3|P-value:4.80E-4|Clearance:0.18||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia2p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; contains WD40 repeats; ortholog of human Ciao1 protein Gene:LSM2(YBL026W)|FD-Score:3.46|P-value:2.72E-4|Clearance:0.02||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MCD4(YKL165C)|FD-Score:3.67|P-value:1.20E-4|Clearance:0.22||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:NOP58(YOR310C)|FD-Score:4.58|P-value:2.32E-6|Clearance:0.02||SGD DESC:Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:NUS1(YDL193W)|FD-Score:-3.49|P-value:2.42E-4|Clearance:0||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PMI40(YER003C)|FD-Score:3.44|P-value:2.89E-4|Clearance:0.03||SGD DESC:Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation Gene:RFC1(YOR217W)|FD-Score:3.71|P-value:1.03E-4|Clearance:0.04||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RPC53(YDL150W)|FD-Score:-3.11|P-value:9.43E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C53 Gene:RPL25(YOL127W)|FD-Score:5.75|P-value:4.45E-9|Clearance:0.95||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPL32(YBL092W)|FD-Score:4.28|P-value:9.33E-6|Clearance:0.03||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:SGV1(YPR161C)|FD-Score:3.33|P-value:4.27E-4|Clearance:0.02||SGD DESC:Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins Gene:SSS1(YDR086C)|FD-Score:4.25|P-value:1.07E-5|Clearance:0.38||SGD DESC:Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p Gene:TFA2(YKR062W)|FD-Score:3.12|P-value:9.00E-4|Clearance:0.1||SGD DESC:TFIIE small subunit, involved in RNA polymerase II transcription initiation Gene:TIM12(YBR091C)|FD-Score:3.32|P-value:4.53E-4|Clearance:0.02||SGD DESC:Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins Gene:TOM40(YMR203W)|FD-Score:4.37|P-value:6.28E-6|Clearance:0.09||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:TRR1(YDR353W)|FD-Score:4.56|P-value:2.52E-6|Clearance:0.2||SGD DESC:Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication Gene:YDL196W(YDL196W_d)|FD-Score:5.53|P-value:1.64E-8|Clearance:0.95||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 467320
Download HOP data (tab-delimited text)  (excel)
Gene:APL5(YPL195W)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function Gene:APL6(YGR261C)|FD-Score:4.87|P-value:5.47E-7||SGD DESC:Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools Gene:APM3(YBR288C)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway Gene:APS3(YJL024C)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress Gene:ASC1(YMR116C)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 that inhibits translation; core component of the small (40S) ribosomal subunit; regulates P-body formation induced by replication stress; represses Gcn4p in the absence of amino acid starvation Gene:ASI1(YMR119W)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; ASI1 has a paralog, ASI3, that arose from the whole genome duplication Gene:ATP5(YDR298C)|FD-Score:-3.45|P-value:2.77E-4||SGD DESC:Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated Gene:ATX1(YNL259C)|FD-Score:4.25|P-value:1.05E-5||SGD DESC:Cytosolic copper metallochaperone that transports copper to the secretory vesicle copper transporter Ccc2p for eventual insertion into Fet3p, which is a multicopper oxidase required for high-affinity iron uptake Gene:BTS1(YPL069C)|FD-Score:3.12|P-value:9.09E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BTT1(YDR252W)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Beta3 subunit of the heterotrimeric nascent polypeptide-associated complex which binds ribosomes via its beta-subunits in close proximity to nascent polypeptides; interacts with Caf130p of the CCR4-NOT complex; similar to human BTF3 Gene:CCC1(YLR220W)|FD-Score:4.53|P-value:2.90E-6||SGD DESC:Putative vacuolar Fe2+/Mn2+ transporter; suppresses respiratory deficit of yfh1 mutants, which lack the ortholog of mammalian frataxin, by preventing mitochondrial iron accumulation; relative distribution to the vacuole decreases upon DNA replication stress Gene:CCC2(YDR270W)|FD-Score:7.28|P-value:1.71E-13||SGD DESC:Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism Gene:CHS5(YLR330W)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane Gene:DAL81(YIR023W)|FD-Score:-4.37|P-value:6.33E-6||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DBF2(YGR092W)|FD-Score:4.16|P-value:1.56E-5||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DIN7(YDR263C)|FD-Score:4.35|P-value:6.70E-6||SGD DESC:Mitochondrial nuclease functioning in DNA repair and replication, modulates the stability of the mitochondrial genome, induced by exposure to mutagens, also induced during meiosis at a time nearly coincident with commitment to recombination Gene:EMP24(YGL200C)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles Gene:ERV14(YGL054C)|FD-Score:7.1|P-value:6.22E-13||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:EUG1(YDR518W)|FD-Score:-4.07|P-value:2.38E-5||SGD DESC:Protein disulfide isomerase of the endoplasmic reticulum lumen; EUG1 has a paralog, PDI1, that arose from the whole genome duplication; function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER Gene:FAA2(YER015W)|FD-Score:3.79|P-value:7.53E-5||SGD DESC:Medium chain fatty acyl-CoA synthetase, activates imported fatty acids; accepts a wide range of fatty acid chain lengths with a preference for medium chains, C9:0-C13:0; localized to the peroxisome Gene:FET3(YMR058W)|FD-Score:8.46|P-value:1.31E-17||SGD DESC:Ferro-O2-oxidoreductase; required for high-affinity iron uptake and involved in mediating resistance to copper ion toxicity, belongs to class of integral membrane multicopper oxidases; protein abundance increases in response to DNA replication stress Gene:GAS4(YOL132W)|FD-Score:3.95|P-value:3.90E-5||SGD DESC:1,3-beta-glucanosyltransferase, involved with Gas2p in spore wall assembly; has similarity to Gas1p; localizes to the cell wall Gene:GCS1(YDL226C)|FD-Score:15.5|P-value:3.52E-54||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GEF1(YJR040W)|FD-Score:8.29|P-value:5.55E-17||SGD DESC:Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism Gene:GSC2(YGR032W)|FD-Score:-3.18|P-value:7.25E-4||SGD DESC:Catalytic subunit of 1,3-beta-glucan synthase, involved in formation of the inner layer of the spore wall; activity positively regulated by Rho1p and negatively by Smk1p; has similarity to an alternate catalytic subunit, Fks1p (Gsc1p) Gene:GTF1(YGR102C)|FD-Score:-3.1|P-value:9.56E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; transposon insertion mutant is salt sensitive and null mutant has growth defects; non-tagged protein is detected in purified mitochondria Gene:GYP1(YOR070C)|FD-Score:4.74|P-value:1.06E-6||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HRQ1(YDR291W_p)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:3'-5'DNA helicase that has DNA strand annealing activity; helicase activity is stimulated by fork structure and 3'-tail length of substrates; belongs to the widely conserved RecQ family of proteins which are involved in maintaining genomic integrity; similar to the human RecQ4p implicated in Rothmund-Thomson syndrome(RTS) Gene:HSM3(YBR272C)|FD-Score:3.16|P-value:7.93E-4||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; related to human 19S subunit S5b; structural study suggests Hsm3p is a scaffold protein for Rpt1p-Rpt2p complex formation Gene:IBA57(YJR122W)|FD-Score:-6.17|P-value:3.43E-10||SGD DESC:Mitochondrial matrix protein involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system Gene:IOC4(YMR044W)|FD-Score:-3.74|P-value:9.12E-5||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing Gene:KHA1(YJL094C)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Putative K+/H+ antiporter with a probable role in intracellular cation homeostasis, localized to Golgi vesicles and detected in highly purified mitochondria in high-throughput studies Gene:LNP1(YHR192W_p)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Lunapark family member involved in ER network formation; localizes to ER junctions and this localization is regulated by the yeast atlastin ortholog Sey1p; interacts with the reticulon protein Rtn1p; induced in response to the DNA-damaging agent MMS Gene:LRG1(YDL240W)|FD-Score:4.08|P-value:2.23E-5||SGD DESC:Putative GTPase-activating protein (GAP) involved in the Pkc1p-mediated signaling pathway that controls cell wall integrity; appears to specifically regulate 1,3-beta-glucan synthesis Gene:MEF1(YLR069C)|FD-Score:-3.3|P-value:4.77E-4||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MFM1(YPL060W)|FD-Score:3.33|P-value:4.42E-4||SGD DESC:Mitochondrial inner membrane magnesium transporter, involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p Gene:MGT1(YDL200C)|FD-Score:4.06|P-value:2.45E-5||SGD DESC:DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage Gene:MRPL22(YNL177C)|FD-Score:-4.11|P-value:1.94E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL35(YDR322W)|FD-Score:-4.18|P-value:1.45E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL51(YPR100W)|FD-Score:-3.17|P-value:7.69E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NHP6A(YPR052C)|FD-Score:3.25|P-value:5.79E-4||SGD DESC:High-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to chromosomes; functionally redundant with Nhp6Bp; homologous to mammalian HMGB1 and HMGB2; NHP6A has a paralog, NHP6B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:NHX1(YDR456W)|FD-Score:4.62|P-value:1.91E-6||SGD DESC:Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion Gene:NMD4(YLR363C)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:Protein that may be involved in nonsense-mediated mRNA decay; interacts with Nam7p, relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:PEP7(YDR323C)|FD-Score:4.6|P-value:2.08E-6||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PET100(YDR079W)|FD-Score:-3.71|P-value:1.03E-4||SGD DESC:Chaperone that specifically facilitates the assembly of cytochrome c oxidase, integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme Gene:PPM1(YDR435C)|FD-Score:3.86|P-value:5.65E-5||SGD DESC:Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits Gene:PUS9(YDL036C)|FD-Score:3.41|P-value:3.28E-4||SGD DESC:Mitochondrial tRNA:pseudouridine synthase, catalyzes the formation of pseudouridine at position 32 in mitochondrial tRNAs; contains an N-terminal mitochondrial targeting sequence Gene:RAV1(YJR033C)|FD-Score:5.44|P-value:2.60E-8||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:REG1(YDR028C)|FD-Score:-3.51|P-value:2.20E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RIM9(YMR063W)|FD-Score:3.4|P-value:3.43E-4||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RMD5(YDR255C)|FD-Score:3.11|P-value:9.39E-4||SGD DESC:Component of GID Complex that confers ubiquitin ligase (U3) activity; necessary for polyubiquitination and degradation of the gluconeogenic enzyme fructose-1,6-bisphosphatase; forms dimer with Fyv10p that is then recruited to GID Complex by Gid8p; also required for sporulation; conserved protein that has a degenerate RING finger domain Gene:RML2(YEL050C)|FD-Score:-3.15|P-value:8.22E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:RPL15B(YMR121C)|FD-Score:-3.49|P-value:2.42E-4||SGD DESC:Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:RRG9(YNL213C)|FD-Score:-4.06|P-value:2.45E-5||SGD DESC:Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RSM18(YER050C)|FD-Score:-3.84|P-value:6.23E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein Gene:RTF1(YGL244W)|FD-Score:-5.47|P-value:2.25E-8||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:SAC3(YDR159W)|FD-Score:-3.65|P-value:1.31E-4||SGD DESC:Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) Gene:SEC22(YLR268W)|FD-Score:5.44|P-value:2.59E-8||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SED1(YDR077W)|FD-Score:3.32|P-value:4.46E-4||SGD DESC:Major stress-induced structural GPI-cell wall glycoprotein in stationary-phase cells, associates with translating ribosomes, possible role in mitochondrial genome maintenance; ORF contains two distinct variable minisatellites Gene:SHE4(YOR035C)|FD-Score:-3.15|P-value:8.03E-4||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SLA1(YBL007C)|FD-Score:-3.89|P-value:5.11E-5||SGD DESC:Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains Gene:SLM5(YCR024C)|FD-Score:-3.2|P-value:6.84E-4||SGD DESC:Mitochondrial asparaginyl-tRNA synthetase Gene:SPF1(YEL031W)|FD-Score:-3.28|P-value:5.21E-4||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SPT2(YER161C)|FD-Score:3.1|P-value:9.59E-4||SGD DESC:Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins Gene:SPT4(YGR063C)|FD-Score:-3.39|P-value:3.54E-4||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:SUR4(YLR372W)|FD-Score:-3.31|P-value:4.63E-4||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SYS1(YJL004C)|FD-Score:7.1|P-value:6.20E-13||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TAT1(YBR069C)|FD-Score:-3.7|P-value:1.07E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:THP1(YOL072W)|FD-Score:-3.33|P-value:4.30E-4||SGD DESC:Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding Gene:TPP1(YMR156C)|FD-Score:-3.12|P-value:9.02E-4||SGD DESC:DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase Gene:TRP3(YKL211C)|FD-Score:-3.32|P-value:4.58E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TVP18(YMR071C)|FD-Score:4.06|P-value:2.46E-5||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:VAC14(YLR386W)|FD-Score:3.31|P-value:4.64E-4||SGD DESC:Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p Gene:VAM6(YDL077C)|FD-Score:5.6|P-value:1.04E-8||SGD DESC:Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p Gene:VMA1(YDL185W)|FD-Score:3.13|P-value:8.72E-4||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VPS20(YMR077C)|FD-Score:3.67|P-value:1.24E-4||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:VPS21(YOR089C)|FD-Score:5.46|P-value:2.41E-8||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS3(YDR495C)|FD-Score:5.38|P-value:3.68E-8||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS41(YDR080W)|FD-Score:7|P-value:1.31E-12||SGD DESC:Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport Gene:YBR074W(YBR074W_p)|FD-Score:3.89|P-value:5.00E-5||SGD DESC:Putative metalloprotease Gene:YBR238C(YBR238C)|FD-Score:-3.44|P-value:2.86E-4||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YDL023C(YDL023C_d)|FD-Score:-4.01|P-value:3.07E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in other Saccharomyces species; overlaps the verified gene GPD1; deletion confers sensitivity to GSAO; deletion in cyr1 mutant results in loss of stress resistance Gene:YDL026W(YDL026W_d)|FD-Score:3.27|P-value:5.30E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR114C(YDR114C_p)|FD-Score:-3.24|P-value:5.91E-4||SGD DESC:Putative protein of unknown function; deletion mutant exhibits poor growth at elevated pH and calcium Gene:YDR179W-A(YDR179W-A_p)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:Putative protein of unknown function Gene:YDR203W(YDR203W_d)|FD-Score:6.78|P-value:5.90E-12||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR269C(YDR269C_d)|FD-Score:10.5|P-value:4.96E-26||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR271C(YDR271C_d)|FD-Score:10.6|P-value:1.30E-26||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W Gene:YDR455C(YDR455C_d)|FD-Score:4.11|P-value:1.94E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YEF1(YEL041W)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication Gene:YGR064W(YGR064W_d)|FD-Score:-4.67|P-value:1.52E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YGR122W(YGR122W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YKL169C(YKL169C_d)|FD-Score:-3.13|P-value:8.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YLR125W(YLR125W_p)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Putative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene Gene:YMR320W(YMR320W_d)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOL118C(YOL118C_d)|FD-Score:4.49|P-value:3.64E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR123C(YPR123C_d)|FD-Score:4.95|P-value:3.64E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPS7(YDR349C)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; located in the cytoplasm and endoplasmic reticulum Gene:YPT31(YER031C)|FD-Score:4.12|P-value:1.90E-5||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication Gene:APL5(YPL195W)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function Gene:APL6(YGR261C)|FD-Score:4.87|P-value:5.47E-7||SGD DESC:Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools Gene:APM3(YBR288C)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway Gene:APS3(YJL024C)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress Gene:ASC1(YMR116C)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 that inhibits translation; core component of the small (40S) ribosomal subunit; regulates P-body formation induced by replication stress; represses Gcn4p in the absence of amino acid starvation Gene:ASI1(YMR119W)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; ASI1 has a paralog, ASI3, that arose from the whole genome duplication Gene:ATP5(YDR298C)|FD-Score:-3.45|P-value:2.77E-4||SGD DESC:Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated Gene:ATX1(YNL259C)|FD-Score:4.25|P-value:1.05E-5||SGD DESC:Cytosolic copper metallochaperone that transports copper to the secretory vesicle copper transporter Ccc2p for eventual insertion into Fet3p, which is a multicopper oxidase required for high-affinity iron uptake Gene:BTS1(YPL069C)|FD-Score:3.12|P-value:9.09E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BTT1(YDR252W)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Beta3 subunit of the heterotrimeric nascent polypeptide-associated complex which binds ribosomes via its beta-subunits in close proximity to nascent polypeptides; interacts with Caf130p of the CCR4-NOT complex; similar to human BTF3 Gene:CCC1(YLR220W)|FD-Score:4.53|P-value:2.90E-6||SGD DESC:Putative vacuolar Fe2+/Mn2+ transporter; suppresses respiratory deficit of yfh1 mutants, which lack the ortholog of mammalian frataxin, by preventing mitochondrial iron accumulation; relative distribution to the vacuole decreases upon DNA replication stress Gene:CCC2(YDR270W)|FD-Score:7.28|P-value:1.71E-13||SGD DESC:Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism Gene:CHS5(YLR330W)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane Gene:DAL81(YIR023W)|FD-Score:-4.37|P-value:6.33E-6||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DBF2(YGR092W)|FD-Score:4.16|P-value:1.56E-5||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DIN7(YDR263C)|FD-Score:4.35|P-value:6.70E-6||SGD DESC:Mitochondrial nuclease functioning in DNA repair and replication, modulates the stability of the mitochondrial genome, induced by exposure to mutagens, also induced during meiosis at a time nearly coincident with commitment to recombination Gene:EMP24(YGL200C)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles Gene:ERV14(YGL054C)|FD-Score:7.1|P-value:6.22E-13||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:EUG1(YDR518W)|FD-Score:-4.07|P-value:2.38E-5||SGD DESC:Protein disulfide isomerase of the endoplasmic reticulum lumen; EUG1 has a paralog, PDI1, that arose from the whole genome duplication; function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER Gene:FAA2(YER015W)|FD-Score:3.79|P-value:7.53E-5||SGD DESC:Medium chain fatty acyl-CoA synthetase, activates imported fatty acids; accepts a wide range of fatty acid chain lengths with a preference for medium chains, C9:0-C13:0; localized to the peroxisome Gene:FET3(YMR058W)|FD-Score:8.46|P-value:1.31E-17||SGD DESC:Ferro-O2-oxidoreductase; required for high-affinity iron uptake and involved in mediating resistance to copper ion toxicity, belongs to class of integral membrane multicopper oxidases; protein abundance increases in response to DNA replication stress Gene:GAS4(YOL132W)|FD-Score:3.95|P-value:3.90E-5||SGD DESC:1,3-beta-glucanosyltransferase, involved with Gas2p in spore wall assembly; has similarity to Gas1p; localizes to the cell wall Gene:GCS1(YDL226C)|FD-Score:15.5|P-value:3.52E-54||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GEF1(YJR040W)|FD-Score:8.29|P-value:5.55E-17||SGD DESC:Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism Gene:GSC2(YGR032W)|FD-Score:-3.18|P-value:7.25E-4||SGD DESC:Catalytic subunit of 1,3-beta-glucan synthase, involved in formation of the inner layer of the spore wall; activity positively regulated by Rho1p and negatively by Smk1p; has similarity to an alternate catalytic subunit, Fks1p (Gsc1p) Gene:GTF1(YGR102C)|FD-Score:-3.1|P-value:9.56E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; transposon insertion mutant is salt sensitive and null mutant has growth defects; non-tagged protein is detected in purified mitochondria Gene:GYP1(YOR070C)|FD-Score:4.74|P-value:1.06E-6||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HRQ1(YDR291W_p)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:3'-5'DNA helicase that has DNA strand annealing activity; helicase activity is stimulated by fork structure and 3'-tail length of substrates; belongs to the widely conserved RecQ family of proteins which are involved in maintaining genomic integrity; similar to the human RecQ4p implicated in Rothmund-Thomson syndrome(RTS) Gene:HSM3(YBR272C)|FD-Score:3.16|P-value:7.93E-4||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; related to human 19S subunit S5b; structural study suggests Hsm3p is a scaffold protein for Rpt1p-Rpt2p complex formation Gene:IBA57(YJR122W)|FD-Score:-6.17|P-value:3.43E-10||SGD DESC:Mitochondrial matrix protein involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system Gene:IOC4(YMR044W)|FD-Score:-3.74|P-value:9.12E-5||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing Gene:KHA1(YJL094C)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Putative K+/H+ antiporter with a probable role in intracellular cation homeostasis, localized to Golgi vesicles and detected in highly purified mitochondria in high-throughput studies Gene:LNP1(YHR192W_p)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Lunapark family member involved in ER network formation; localizes to ER junctions and this localization is regulated by the yeast atlastin ortholog Sey1p; interacts with the reticulon protein Rtn1p; induced in response to the DNA-damaging agent MMS Gene:LRG1(YDL240W)|FD-Score:4.08|P-value:2.23E-5||SGD DESC:Putative GTPase-activating protein (GAP) involved in the Pkc1p-mediated signaling pathway that controls cell wall integrity; appears to specifically regulate 1,3-beta-glucan synthesis Gene:MEF1(YLR069C)|FD-Score:-3.3|P-value:4.77E-4||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MFM1(YPL060W)|FD-Score:3.33|P-value:4.42E-4||SGD DESC:Mitochondrial inner membrane magnesium transporter, involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p Gene:MGT1(YDL200C)|FD-Score:4.06|P-value:2.45E-5||SGD DESC:DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage Gene:MRPL22(YNL177C)|FD-Score:-4.11|P-value:1.94E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL35(YDR322W)|FD-Score:-4.18|P-value:1.45E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL51(YPR100W)|FD-Score:-3.17|P-value:7.69E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NHP6A(YPR052C)|FD-Score:3.25|P-value:5.79E-4||SGD DESC:High-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to chromosomes; functionally redundant with Nhp6Bp; homologous to mammalian HMGB1 and HMGB2; NHP6A has a paralog, NHP6B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:NHX1(YDR456W)|FD-Score:4.62|P-value:1.91E-6||SGD DESC:Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion Gene:NMD4(YLR363C)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:Protein that may be involved in nonsense-mediated mRNA decay; interacts with Nam7p, relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:PEP7(YDR323C)|FD-Score:4.6|P-value:2.08E-6||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PET100(YDR079W)|FD-Score:-3.71|P-value:1.03E-4||SGD DESC:Chaperone that specifically facilitates the assembly of cytochrome c oxidase, integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme Gene:PPM1(YDR435C)|FD-Score:3.86|P-value:5.65E-5||SGD DESC:Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits Gene:PUS9(YDL036C)|FD-Score:3.41|P-value:3.28E-4||SGD DESC:Mitochondrial tRNA:pseudouridine synthase, catalyzes the formation of pseudouridine at position 32 in mitochondrial tRNAs; contains an N-terminal mitochondrial targeting sequence Gene:RAV1(YJR033C)|FD-Score:5.44|P-value:2.60E-8||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:REG1(YDR028C)|FD-Score:-3.51|P-value:2.20E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RIM9(YMR063W)|FD-Score:3.4|P-value:3.43E-4||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RMD5(YDR255C)|FD-Score:3.11|P-value:9.39E-4||SGD DESC:Component of GID Complex that confers ubiquitin ligase (U3) activity; necessary for polyubiquitination and degradation of the gluconeogenic enzyme fructose-1,6-bisphosphatase; forms dimer with Fyv10p that is then recruited to GID Complex by Gid8p; also required for sporulation; conserved protein that has a degenerate RING finger domain Gene:RML2(YEL050C)|FD-Score:-3.15|P-value:8.22E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:RPL15B(YMR121C)|FD-Score:-3.49|P-value:2.42E-4||SGD DESC:Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:RRG9(YNL213C)|FD-Score:-4.06|P-value:2.45E-5||SGD DESC:Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RSM18(YER050C)|FD-Score:-3.84|P-value:6.23E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein Gene:RTF1(YGL244W)|FD-Score:-5.47|P-value:2.25E-8||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:SAC3(YDR159W)|FD-Score:-3.65|P-value:1.31E-4||SGD DESC:Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) Gene:SEC22(YLR268W)|FD-Score:5.44|P-value:2.59E-8||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SED1(YDR077W)|FD-Score:3.32|P-value:4.46E-4||SGD DESC:Major stress-induced structural GPI-cell wall glycoprotein in stationary-phase cells, associates with translating ribosomes, possible role in mitochondrial genome maintenance; ORF contains two distinct variable minisatellites Gene:SHE4(YOR035C)|FD-Score:-3.15|P-value:8.03E-4||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SLA1(YBL007C)|FD-Score:-3.89|P-value:5.11E-5||SGD DESC:Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains Gene:SLM5(YCR024C)|FD-Score:-3.2|P-value:6.84E-4||SGD DESC:Mitochondrial asparaginyl-tRNA synthetase Gene:SPF1(YEL031W)|FD-Score:-3.28|P-value:5.21E-4||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SPT2(YER161C)|FD-Score:3.1|P-value:9.59E-4||SGD DESC:Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins Gene:SPT4(YGR063C)|FD-Score:-3.39|P-value:3.54E-4||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:SUR4(YLR372W)|FD-Score:-3.31|P-value:4.63E-4||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SYS1(YJL004C)|FD-Score:7.1|P-value:6.20E-13||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TAT1(YBR069C)|FD-Score:-3.7|P-value:1.07E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:THP1(YOL072W)|FD-Score:-3.33|P-value:4.30E-4||SGD DESC:Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding Gene:TPP1(YMR156C)|FD-Score:-3.12|P-value:9.02E-4||SGD DESC:DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase Gene:TRP3(YKL211C)|FD-Score:-3.32|P-value:4.58E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TVP18(YMR071C)|FD-Score:4.06|P-value:2.46E-5||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:VAC14(YLR386W)|FD-Score:3.31|P-value:4.64E-4||SGD DESC:Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p Gene:VAM6(YDL077C)|FD-Score:5.6|P-value:1.04E-8||SGD DESC:Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p Gene:VMA1(YDL185W)|FD-Score:3.13|P-value:8.72E-4||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VPS20(YMR077C)|FD-Score:3.67|P-value:1.24E-4||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:VPS21(YOR089C)|FD-Score:5.46|P-value:2.41E-8||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS3(YDR495C)|FD-Score:5.38|P-value:3.68E-8||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS41(YDR080W)|FD-Score:7|P-value:1.31E-12||SGD DESC:Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport Gene:YBR074W(YBR074W_p)|FD-Score:3.89|P-value:5.00E-5||SGD DESC:Putative metalloprotease Gene:YBR238C(YBR238C)|FD-Score:-3.44|P-value:2.86E-4||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YDL023C(YDL023C_d)|FD-Score:-4.01|P-value:3.07E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in other Saccharomyces species; overlaps the verified gene GPD1; deletion confers sensitivity to GSAO; deletion in cyr1 mutant results in loss of stress resistance Gene:YDL026W(YDL026W_d)|FD-Score:3.27|P-value:5.30E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR114C(YDR114C_p)|FD-Score:-3.24|P-value:5.91E-4||SGD DESC:Putative protein of unknown function; deletion mutant exhibits poor growth at elevated pH and calcium Gene:YDR179W-A(YDR179W-A_p)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:Putative protein of unknown function Gene:YDR203W(YDR203W_d)|FD-Score:6.78|P-value:5.90E-12||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR269C(YDR269C_d)|FD-Score:10.5|P-value:4.96E-26||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR271C(YDR271C_d)|FD-Score:10.6|P-value:1.30E-26||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W Gene:YDR455C(YDR455C_d)|FD-Score:4.11|P-value:1.94E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YEF1(YEL041W)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication Gene:YGR064W(YGR064W_d)|FD-Score:-4.67|P-value:1.52E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YGR122W(YGR122W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YKL169C(YKL169C_d)|FD-Score:-3.13|P-value:8.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YLR125W(YLR125W_p)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Putative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene Gene:YMR320W(YMR320W_d)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOL118C(YOL118C_d)|FD-Score:4.49|P-value:3.64E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR123C(YPR123C_d)|FD-Score:4.95|P-value:3.64E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPS7(YDR349C)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; located in the cytoplasm and endoplasmic reticulum Gene:YPT31(YER031C)|FD-Score:4.12|P-value:1.90E-5||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOL127W5.754.45E-90.94RPL25Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23
YDL196W_d5.531.64E-80.94YDL196W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
YOR310C4.582.32E-60.02NOP58Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA
YDR353W4.562.52E-60.20TRR1Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication
YMR203W4.376.28E-60.09TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YBL092W4.289.33E-60.03RPL32Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog
YDR086C4.251.07E-50.38SSS1Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p
YOR257W3.875.47E-50.16CDC31Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation
YOR217W3.711.03E-40.04RFC1Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
YKL165C3.671.20E-40.21MCD4Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes
YBL026W3.462.72E-40.02LSM2Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YER003C3.442.89E-40.03PMI40Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation
YNL161W3.413.23E-40.08CBK1Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress
YPR161C3.334.27E-40.02SGV1Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins
YBR091C3.324.53E-40.02TIM12Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDL226C15.503.52E-54GCS1ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p
YDR271C_d10.601.30E-26YDR271C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W
YDR269C_d10.504.96E-26YDR269C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YMR058W8.461.31E-17FET3Ferro-O2-oxidoreductase; required for high-affinity iron uptake and involved in mediating resistance to copper ion toxicity, belongs to class of integral membrane multicopper oxidases; protein abundance increases in response to DNA replication stress
YJR040W8.295.55E-17GEF1Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism
YDR270W7.281.71E-13CCC2Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism
YJL004C7.106.20E-13SYS1Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation
YGL054C7.106.22E-13ERV14COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication
YDR080W7.001.31E-12VPS41Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport
YDR203W_d6.785.90E-12YDR203W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YDL077C5.601.04E-8VAM6Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p
YOR089C5.462.41E-8VPS21Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication
YLR268W5.442.59E-8SEC22R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog
YJR033C5.442.60E-8RAV1Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate
YDR495C5.383.68E-8VPS3Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase

GO enrichment analysis for SGTC_2675
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.4156.51E-245SGTC_1658st012944 20.0 μMTimTec (Natural product derivative library)242072600.0555556iron homeostasis
0.4109.61E-239SGTC_22917917025 104.3 μMChembridge (Fragment library)29646120.0752688iron homeostasis
0.4058.38E-232SGTC_2666tannic acid 100.0 μMMicrosource (Natural product library)161342670.25iron homeostasis
0.3891.62E-212SGTC_22757947463 200.0 μMChembridge (Fragment library)12254140.0795455iron homeostasis
0.3821.37E-204SGTC_11103-hydroxy-3'-methoxyflavone 1.2 μMTimTec (Natural product library)6762960.127907iron homeostasis
0.3716.55E-192SGTC_263alizarin 107.9 μMMiscellaneous62930.152778iron homeostasis
0.3394.19E-159SGTC_23899091542 195.5 μMChembridge (Fragment library)36266240.0574713iron homeostasis
0.3307.78E-150SGTC_3740880-0231 82.3 μMChemDiv (Drug-like library)68335870.0891089iron homeostasis
0.3241.15E-144SGTC_2586curcumin 80.0 μMICCB bioactive library66103320.107527iron homeostasis
0.2839.39E-109SGTC_21535569715 200.0 μMChembridge (Fragment library)20547010.0823529iron homeostasis
0.2741.88E-102SGTC_20464110841 106.0 μMChembridge (Fragment library)441196300.0379747iron homeostasis
0.2681.13E-97SGTC_444curcumin 90.4 μMICCB bioactive library66103320.107527iron homeostasis
0.2611.23E-92SGTC_8750709-0210 25.7 μMChemDiv (Drug-like library)62513640.0555556fatty acid desaturase (OLE1)
0.2611.39E-92SGTC_3393091-4833 71.4 μMChemDiv (Drug-like library)32400770.0674157iron homeostasis
0.2402.25E-78SGTC_1660st012960 28.1 μMTimTec (Natural product derivative library)242072660.0978261iron homeostasis

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_402epigallocatechin gallate10 μM0.61904865064Miscellaneous458.371723.097811
SGTC_2509methyl 7-deshydroxypyrogallin-4-carboxylate54.59 μM0.2533335098884Microsource (Natural product library)262.21490.02636RPP1 & pyrimidine depletion
SGTC_2615purpurogallin-4-carboxylic acid100 μM0.25269315Microsource (Natural product library)264.18772-0.73757redox potentiating
SGTC_2666tannic acid100 μM0.2516134267Microsource (Natural product library)1701.198488.8132546iron homeostasis
SGTC_1529apigenin74 μM0.2133335280443TimTec (Pure natural product library)270.23692.4135
SGTC_2522biochanin a4.94 μM0.21255280373Microsource (Natural product library)284.263482.36625RNA pol III & RNase P/MRP
SGTC_1115galangin695.68 nM0.2105265281616TimTec (Natural product library)270.23692.11435RPP1 & pyrimidine depletion
SGTC_2505galangin7.89 μM0.2105265281616Microsource (Natural product library)270.23692.11435RPP1 & pyrimidine depletion
SGTC_1562morin hydrate59.1 μM0.21052618542136TimTec (Pure natural product library)338.266261.21679
SGTC_2515chrysin20.77 μM0.2105265281607Microsource (Natural product library)254.23752.65224RPP1 & pyrimidine depletion
SGTC_26335,7-dihydroxyisoflavone100 μM0.2105265377381Microsource (Natural product library)254.23752.38224