mitomycin C FDA approved compound

Mitomycin is a bifunctional and trifunctional alkylating agent, cross-links DNA inhibiting DNA synthesis and function. Mitomycin is cell cycle phase-nonspecific.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2677
Screen concentration 100.0 μM
Source Miscellaneous
PubChem CID 5746
SMILES CC1=C(C(=O)C2=C(C1=O)N3CC4C(C3(C2COC(=O)N)OC)N4)N
Standardized SMILES COC12C3NC3CN1C4=C(C2COC(=O)N)C(=O)C(=C(C)C4=O)N
Molecular weight 334.3272
ALogP -1.55
H-bond donor count 3
H-bond acceptor count 8
Response signature DNA damage response

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.61
% growth inhibition (Hom. pool) 4.49


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5746
Download HIP data (tab-delimited text)  (excel)
Gene:ARP2(YDL029W)|FD-Score:3.4|P-value:3.43E-4|Clearance:0.19||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:IRA1(YBR140C)|FD-Score:3.48|P-value:2.51E-4|Clearance:0.08||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:LAS17(YOR181W)|FD-Score:3.2|P-value:6.98E-4|Clearance:0.18||SGD DESC:Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP) Gene:LSM2(YBL026W)|FD-Score:4.34|P-value:7.04E-6|Clearance:0.4||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:RPB7(YDR404C)|FD-Score:3.85|P-value:5.94E-5|Clearance:0.37||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPC19(YNL113W)|FD-Score:3.21|P-value:6.68E-4|Clearance:0.01||SGD DESC:RNA polymerase subunit AC19, common to RNA polymerases I and III Gene:SFH1(YLR321C)|FD-Score:3.94|P-value:4.07E-5|Clearance:0.09||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:ARP2(YDL029W)|FD-Score:3.4|P-value:3.43E-4|Clearance:0.19||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:IRA1(YBR140C)|FD-Score:3.48|P-value:2.51E-4|Clearance:0.08||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:LAS17(YOR181W)|FD-Score:3.2|P-value:6.98E-4|Clearance:0.18||SGD DESC:Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP) Gene:LSM2(YBL026W)|FD-Score:4.34|P-value:7.04E-6|Clearance:0.4||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:RPB7(YDR404C)|FD-Score:3.85|P-value:5.94E-5|Clearance:0.37||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPC19(YNL113W)|FD-Score:3.21|P-value:6.68E-4|Clearance:0.01||SGD DESC:RNA polymerase subunit AC19, common to RNA polymerases I and III Gene:SFH1(YLR321C)|FD-Score:3.94|P-value:4.07E-5|Clearance:0.09||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5746
Download HOP data (tab-delimited text)  (excel)
Gene:CDC26(YFR036W)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CHD1(YER164W)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Chromatin remodeler that regulates various aspects of transcription; acts in in conjunction with Isw1b to regulate chromatin structure and maintain chromatin integrity during transcription elongation by RNAP II by preventing trans-histone exchange over coding regions; contains a chromo domain, a helicase domain and a DNA-binding domain; component of both the SAGA and SLIK complexes Gene:CMC1(YKL137W)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Evolutionarily conserved copper-binding protein of the mitochondrial intermembrane space, may be involved in delivering copper from the matrix to the cytochrome c oxidase complex; contains a twin CX9C motif Gene:COS111(YBR203W)|FD-Score:5.28|P-value:6.41E-8||SGD DESC:Protein required for resistance to the antifungal drug ciclopirox olamine; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FUN26(YAL022C)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Vacuolar membrane transporter with broad nucleoside selectivity; may regulate balance of nicotinamide riboside (NmR) levels between cytosol and vacuole, contributing to salvage of NmR for use in cytosolic NAD+ synthesis Gene:GLY1(YEL046C)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:HRQ1(YDR291W_p)|FD-Score:6.35|P-value:1.08E-10||SGD DESC:3'-5'DNA helicase that has DNA strand annealing activity; helicase activity is stimulated by fork structure and 3'-tail length of substrates; belongs to the widely conserved RecQ family of proteins which are involved in maintaining genomic integrity; similar to the human RecQ4p implicated in Rothmund-Thomson syndrome(RTS) Gene:IDP1(YDL066W)|FD-Score:4.39|P-value:5.60E-6||SGD DESC:Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes Gene:MDM12(YOL009C)|FD-Score:3.85|P-value:5.87E-5||SGD DESC:Mitochondrial outer membrane protein, required for transmission of mitochondria to daughter cells; component of the ERMES complex that links the ER to mitochondria; may influence import and assembly of outer membrane beta-barrel proteins Gene:MMS2(YGL087C)|FD-Score:6.13|P-value:4.53E-10||SGD DESC:Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress Gene:MPH1(YIR002C)|FD-Score:4.23|P-value:1.16E-5||SGD DESC:3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds to flap DNA in an error-free bypass pathway and stimulates the activity of Rad27p and Dna2p; also involved in interstrand cross-link repair mutations confer a mutator phenotype; similarity to FANCM, a human Fanconi anemia complementation group protein that along with the MHF complex is involved in stabilizing and remodeling blocked replication forks; member of the SF2 DExD/H superfamily of helicases Gene:PHO80(YOL001W)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling Gene:PSO2(YMR137C)|FD-Score:4.59|P-value:2.23E-6||SGD DESC:Nuclease required for DNA single- and double-strand break repair; acts at a post-incision step in repair of breaks that result from interstrand cross-links produced by a variety of mono- and bi-functional psoralen derivatives; induced by UV-irradiation; forms nuclear foci upon DNA replication stress Gene:RAD1(YPL022W)|FD-Score:5.32|P-value:5.08E-8||SGD DESC:Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein Gene:RAD10(YML095C)|FD-Score:6.99|P-value:1.33E-12||SGD DESC:Single-stranded DNA endonuclease (with Rad1p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein Gene:RAD14(YMR201C)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein Gene:RAD18(YCR066W)|FD-Score:5.27|P-value:6.77E-8||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RAD2(YGR258C)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein Gene:RAD4(YER162C)|FD-Score:3.14|P-value:8.33E-4||SGD DESC:Protein that recognizes and binds damaged DNA (with Rad23p) during nucleotide excision repair; subunit of Nuclear Excision Repair Factor 2 (NEF2); also involved, with Rad23p, in turnover of ubiquitylated proteins Gene:RAD5(YLR032W)|FD-Score:7.02|P-value:1.14E-12||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:RAD51(YER095W)|FD-Score:4.11|P-value:1.98E-5||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RAD54(YGL163C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress Gene:RAD55(YDR076W)|FD-Score:7.21|P-value:2.80E-13||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p Gene:RAD57(YDR004W)|FD-Score:5.27|P-value:6.75E-8||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p Gene:RPL31A(YDL075W)|FD-Score:3.1|P-value:9.82E-4||SGD DESC:Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication Gene:RPS16A(YMR143W)|FD-Score:-4.78|P-value:8.75E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16A has a paralog, RPS16B, that arose from the whole genome duplication Gene:SAE3(YHR079C-A)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:Meiosis specific protein involved in DMC1-dependent meiotic recombination, forms heterodimer with Mei5p; proposed to be an assembly factor for Dmc1p Gene:UBC13(YDR092W)|FD-Score:4.74|P-value:1.05E-6||SGD DESC:Ubiquitin-conjugating enzyme involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus Gene:YBL029C-A(YBL029C-A_p)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress; has potential orthologs in Saccharomyces species and in Yarrowia lipolytica Gene:YBL095W(YBL095W_p)|FD-Score:3.75|P-value:8.88E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YDR271C(YDR271C_d)|FD-Score:3.17|P-value:7.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W Gene:YOR121C(YOR121C_d)|FD-Score:3.18|P-value:7.31E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GCY1/YOR120W Gene:CDC26(YFR036W)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CHD1(YER164W)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Chromatin remodeler that regulates various aspects of transcription; acts in in conjunction with Isw1b to regulate chromatin structure and maintain chromatin integrity during transcription elongation by RNAP II by preventing trans-histone exchange over coding regions; contains a chromo domain, a helicase domain and a DNA-binding domain; component of both the SAGA and SLIK complexes Gene:CMC1(YKL137W)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Evolutionarily conserved copper-binding protein of the mitochondrial intermembrane space, may be involved in delivering copper from the matrix to the cytochrome c oxidase complex; contains a twin CX9C motif Gene:COS111(YBR203W)|FD-Score:5.28|P-value:6.41E-8||SGD DESC:Protein required for resistance to the antifungal drug ciclopirox olamine; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FUN26(YAL022C)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Vacuolar membrane transporter with broad nucleoside selectivity; may regulate balance of nicotinamide riboside (NmR) levels between cytosol and vacuole, contributing to salvage of NmR for use in cytosolic NAD+ synthesis Gene:GLY1(YEL046C)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:HRQ1(YDR291W_p)|FD-Score:6.35|P-value:1.08E-10||SGD DESC:3'-5'DNA helicase that has DNA strand annealing activity; helicase activity is stimulated by fork structure and 3'-tail length of substrates; belongs to the widely conserved RecQ family of proteins which are involved in maintaining genomic integrity; similar to the human RecQ4p implicated in Rothmund-Thomson syndrome(RTS) Gene:IDP1(YDL066W)|FD-Score:4.39|P-value:5.60E-6||SGD DESC:Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes Gene:MDM12(YOL009C)|FD-Score:3.85|P-value:5.87E-5||SGD DESC:Mitochondrial outer membrane protein, required for transmission of mitochondria to daughter cells; component of the ERMES complex that links the ER to mitochondria; may influence import and assembly of outer membrane beta-barrel proteins Gene:MMS2(YGL087C)|FD-Score:6.13|P-value:4.53E-10||SGD DESC:Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress Gene:MPH1(YIR002C)|FD-Score:4.23|P-value:1.16E-5||SGD DESC:3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds to flap DNA in an error-free bypass pathway and stimulates the activity of Rad27p and Dna2p; also involved in interstrand cross-link repair mutations confer a mutator phenotype; similarity to FANCM, a human Fanconi anemia complementation group protein that along with the MHF complex is involved in stabilizing and remodeling blocked replication forks; member of the SF2 DExD/H superfamily of helicases Gene:PHO80(YOL001W)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling Gene:PSO2(YMR137C)|FD-Score:4.59|P-value:2.23E-6||SGD DESC:Nuclease required for DNA single- and double-strand break repair; acts at a post-incision step in repair of breaks that result from interstrand cross-links produced by a variety of mono- and bi-functional psoralen derivatives; induced by UV-irradiation; forms nuclear foci upon DNA replication stress Gene:RAD1(YPL022W)|FD-Score:5.32|P-value:5.08E-8||SGD DESC:Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein Gene:RAD10(YML095C)|FD-Score:6.99|P-value:1.33E-12||SGD DESC:Single-stranded DNA endonuclease (with Rad1p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein Gene:RAD14(YMR201C)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein Gene:RAD18(YCR066W)|FD-Score:5.27|P-value:6.77E-8||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RAD2(YGR258C)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein Gene:RAD4(YER162C)|FD-Score:3.14|P-value:8.33E-4||SGD DESC:Protein that recognizes and binds damaged DNA (with Rad23p) during nucleotide excision repair; subunit of Nuclear Excision Repair Factor 2 (NEF2); also involved, with Rad23p, in turnover of ubiquitylated proteins Gene:RAD5(YLR032W)|FD-Score:7.02|P-value:1.14E-12||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:RAD51(YER095W)|FD-Score:4.11|P-value:1.98E-5||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RAD54(YGL163C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress Gene:RAD55(YDR076W)|FD-Score:7.21|P-value:2.80E-13||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p Gene:RAD57(YDR004W)|FD-Score:5.27|P-value:6.75E-8||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p Gene:RPL31A(YDL075W)|FD-Score:3.1|P-value:9.82E-4||SGD DESC:Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication Gene:RPS16A(YMR143W)|FD-Score:-4.78|P-value:8.75E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16A has a paralog, RPS16B, that arose from the whole genome duplication Gene:SAE3(YHR079C-A)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:Meiosis specific protein involved in DMC1-dependent meiotic recombination, forms heterodimer with Mei5p; proposed to be an assembly factor for Dmc1p Gene:UBC13(YDR092W)|FD-Score:4.74|P-value:1.05E-6||SGD DESC:Ubiquitin-conjugating enzyme involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus Gene:YBL029C-A(YBL029C-A_p)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress; has potential orthologs in Saccharomyces species and in Yarrowia lipolytica Gene:YBL095W(YBL095W_p)|FD-Score:3.75|P-value:8.88E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YDR271C(YDR271C_d)|FD-Score:3.17|P-value:7.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W Gene:YOR121C(YOR121C_d)|FD-Score:3.18|P-value:7.31E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GCY1/YOR120W

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBL026W4.347.04E-60.40LSM2Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YLR321C3.944.07E-50.09SFH1Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog
YDR404C3.855.94E-50.37RPB7RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation
YBR140C3.482.51E-40.08IRA1GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication
YDL029W3.403.43E-40.19ARP2Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity
YNL113W3.216.68E-40.01RPC19RNA polymerase subunit AC19, common to RNA polymerases I and III
YOR181W3.206.98E-40.18LAS17Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP)
YPR144C3.020.001280.11NOC4Nucleolar protein, forms a complex with Nop14p that mediates maturation and nuclear export of 40S ribosomal subunits
YBL092W2.900.001860.02RPL32Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog
YMR211W2.880.002010.26DML1Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
YNL126W2.610.004490.04SPC98Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
YFR037C2.570.005110.02RSC8Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
YDR394W2.550.005420.01RPT3One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B
YPR104C2.540.005510.00FHL1Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants
YGR253C2.540.005550.01PUP2Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR076W7.212.80E-13RAD55Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p
YLR032W7.021.14E-12RAD5DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress
YML095C6.991.33E-12RAD10Single-stranded DNA endonuclease (with Rad1p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein
YDR291W_p6.351.08E-10HRQ1_p3'-5'DNA helicase that has DNA strand annealing activity; helicase activity is stimulated by fork structure and 3'-tail length of substrates; belongs to the widely conserved RecQ family of proteins which are involved in maintaining genomic integrity; similar to the human RecQ4p implicated in Rothmund-Thomson syndrome(RTS)
YGL087C6.134.53E-10MMS2Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress
YPL022W5.325.08E-8RAD1Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein
YBR203W5.286.41E-8COS111Protein required for resistance to the antifungal drug ciclopirox olamine; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YDR004W5.276.75E-8RAD57Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p
YCR066W5.276.77E-8RAD18E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA
YDR092W4.741.05E-6UBC13Ubiquitin-conjugating enzyme involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus
YMR137C4.592.23E-6PSO2Nuclease required for DNA single- and double-strand break repair; acts at a post-incision step in repair of breaks that result from interstrand cross-links produced by a variety of mono- and bi-functional psoralen derivatives; induced by UV-irradiation; forms nuclear foci upon DNA replication stress
YDL066W4.395.60E-6IDP1Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes
YIR002C4.231.16E-5MPH13'-5' DNA helicase involved in error-free bypass of DNA lesions; binds to flap DNA in an error-free bypass pathway and stimulates the activity of Rad27p and Dna2p; also involved in interstrand cross-link repair mutations confer a mutator phenotype; similarity to FANCM, a human Fanconi anemia complementation group protein that along with the MHF complex is involved in stabilizing and remodeling blocked replication forks; member of the SF2 DExD/H superfamily of helicases
YER095W4.111.98E-5RAD51Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein
YOL009C3.855.87E-5MDM12Mitochondrial outer membrane protein, required for transmission of mitochondria to daughter cells; component of the ERMES complex that links the ER to mitochondria; may influence import and assembly of outer membrane beta-barrel proteins

GO enrichment analysis for SGTC_2677
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.5200SGTC_2674-Nitroquinoline-1-oxide (4-NQO) 44.4 nMMiscellaneous59550.0263158DNA damage response
0.3231.65E-143SGTC_247mechlorethamine 29.7 μMMiscellaneous221335930.0327869DNA damage response
0.2519.01E-86SGTC_14254048-4433 7.3 μMChemDiv (Drug-like library)237349460.0222222copper-dependent oxidative stress
0.2481.82E-83SGTC_248streptozotocin 5.2 mMMiscellaneous53000.0740741DNA damage response
0.2468.80E-82SGTC_915methyl methanesulfonate (MMS) 110.0 mMMiscellaneous41560.0701754DNA damage response
0.2362.99E-75SGTC_1936st074407 74.3 μMTimTec (Natural product derivative library)8098130.0581395DNA damage response
0.2151.66E-62SGTC_5504123-0385 25.7 μMChemDiv (Drug-like library)57311230.0652174TSC3-RPN4
0.1999.48E-54SGTC_32549136651 49.5 μMChembridge (Drug-like library)164540300.0833333DNA damage response
0.1965.65E-52SGTC_1079idarubicin 2.4 μMNIH Clinical Collection6363620.12DNA damage response
0.1932.66E-50SGTC_244nsc-207895 1.3 μMMiscellaneous426400.0574713DNA damage response
0.1822.72E-45SGTC_4291112-0049 25.5 μMChemDiv (Drug-like library)28361700.045977DNA damage response
0.1818.91E-45SGTC_32479135149 49.5 μMChembridge (Drug-like library)89344360.0133333DNA damage response
0.1776.03E-43SGTC_8291635-0120 117.0 μMChemDiv (Drug-like library)2850540.012987DNA damage response
0.1633.13E-36SGTC_558k064-0027 21.3 μMChemDiv (Drug-like library)50885260.0481928DNA damage response
0.1628.88E-36SGTC_25354',4'-dimethoxydalbergione 51.2 μMMicrosource (Natural product library)3641060.075DNA damage response

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2395hr heat shock (37°C) + mitomycin C819.44 μM15746Miscellaneous334.32722-1.54738heat shock/prefoldin
SGTC_420mitomycin C81.9 mM15746Miscellaneous334.32722-1.54738
SGTC_1639Lupinine o-methylbenzoicacid ester hydrochloride61.8 μM0.162517037758TimTec (Natural product derivative library)323.857544.19413ergosterol biosynthesis
SGTC_1660st01296028.1 μM0.162524207266TimTec (Natural product derivative library)580.4471550.052815iron homeostasis
SGTC_22907904846200 μM0.1621622962154Chembridge (Fragment library)280.243612.17216
SGTC_2712st07912647.7 μM0.1590913152970TimTec (Natural product derivative library)419.426642.51617
SGTC_2894904862532.47 μM0.1558444092861Chembridge (Drug-like library)278.346781.38214
SGTC_1877wortmannin25 μM0.153061312145ICCB bioactive library428.431861.70508
SGTC_2422chlorphenesin carbamate384.24 μM0.1527782724Miscellaneous245.659581.59724
SGTC_2946906550271.43 μM0.15116316489101Chembridge (Drug-like library)324.37372.35613