equilin

3-hydroxy-13-methyl-9,11,12,14,15,16-hexahydro-6H-cyclopenta[a]phenanthren-17-one

Equilin is an estrone commonly used hormone replacement therapy

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2678
Screen concentration 100.0 μM
Source Microsource (Natural product library)
PubChem CID 3247
SMILES CC12CCC3C(=CCC4=C3C=CC(=C4)O)C1CCC2=O
Standardized SMILES CC12CCC3C(=CCc4cc(O)ccc34)C1CCC2=O
Molecular weight 268.3502
ALogP 3.69
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 12.18
% growth inhibition (Hom. pool) 10.43


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3247
Download HIP data (tab-delimited text)  (excel)
Gene:CEF1(YMR213W)|FD-Score:3.37|P-value:3.71E-4|Clearance:0.31||SGD DESC:Essential splicing factor; associated with Prp19p and the spliceosome, contains an N-terminal c-Myb DNA binding motif necessary for cell viability but not for Prp19p association, evolutionarily conserved and homologous to S. pombe Cdc5p Gene:HYP2(YEL034W)|FD-Score:-3.47|P-value:2.61E-4|Clearance:0||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:LAS17(YOR181W)|FD-Score:3.6|P-value:1.59E-4|Clearance:0.31||SGD DESC:Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP) Gene:LCB2(YDR062W)|FD-Score:-3.94|P-value:4.12E-5|Clearance:0||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:PRP46(YPL151C)|FD-Score:3.41|P-value:3.23E-4|Clearance:0.31||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs Gene:PSF2(YJL072C)|FD-Score:-3.23|P-value:6.16E-4|Clearance:0||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:PUP1(YOR157C)|FD-Score:-3.16|P-value:7.99E-4|Clearance:0||SGD DESC:Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z Gene:RPB8(YOR224C)|FD-Score:-3.21|P-value:6.56E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPN6(YDL097C)|FD-Score:-3.62|P-value:1.50E-4|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:RPT4(YOR259C)|FD-Score:-3.45|P-value:2.75E-4|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization Gene:RSC58(YLR033W)|FD-Score:-3.13|P-value:8.77E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:YDR526C(YDR526C_d)|FD-Score:3.73|P-value:9.41E-5|Clearance:0.31||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:CEF1(YMR213W)|FD-Score:3.37|P-value:3.71E-4|Clearance:0.31||SGD DESC:Essential splicing factor; associated with Prp19p and the spliceosome, contains an N-terminal c-Myb DNA binding motif necessary for cell viability but not for Prp19p association, evolutionarily conserved and homologous to S. pombe Cdc5p Gene:HYP2(YEL034W)|FD-Score:-3.47|P-value:2.61E-4|Clearance:0||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:LAS17(YOR181W)|FD-Score:3.6|P-value:1.59E-4|Clearance:0.31||SGD DESC:Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP) Gene:LCB2(YDR062W)|FD-Score:-3.94|P-value:4.12E-5|Clearance:0||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:PRP46(YPL151C)|FD-Score:3.41|P-value:3.23E-4|Clearance:0.31||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs Gene:PSF2(YJL072C)|FD-Score:-3.23|P-value:6.16E-4|Clearance:0||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:PUP1(YOR157C)|FD-Score:-3.16|P-value:7.99E-4|Clearance:0||SGD DESC:Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z Gene:RPB8(YOR224C)|FD-Score:-3.21|P-value:6.56E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPN6(YDL097C)|FD-Score:-3.62|P-value:1.50E-4|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:RPT4(YOR259C)|FD-Score:-3.45|P-value:2.75E-4|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization Gene:RSC58(YLR033W)|FD-Score:-3.13|P-value:8.77E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:YDR526C(YDR526C_d)|FD-Score:3.73|P-value:9.41E-5|Clearance:0.31||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3247
Download HOP data (tab-delimited text)  (excel)
Gene:ACM1(YPL267W)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:Pseudosubstrate inhibitor of the anaphase-promoting complex/cyclosome (APC/C), that suppresses APC/C [Cdh1]-mediated proteolysis of mitotic cyclins; associates with Cdh1p, Bmh1p and Bmh2p; cell cycle regulated protein Gene:ALE1(YOR175C)|FD-Score:3.24|P-value:6.04E-4||SGD DESC:Broad-specificity lysophospholipid acyltransferase, part of MBOAT family of membrane-bound O-acyltransferases; key component of Lands cycle; may have role in fatty acid exchange at sn-2 position of mature glycerophospholipids Gene:ARO1(YDR127W)|FD-Score:4.68|P-value:1.46E-6||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO3(YDR035W)|FD-Score:3.89|P-value:5.10E-5||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ATF1(YOR377W)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Alcohol acetyltransferase; responsible for the major part of volatile acetate ester production during fermentation; main enzyme involved in terpenyl acetate synthesis; potential roles in lipid and sterol metabolism Gene:ATG32(YIL146C)|FD-Score:3.12|P-value:9.12E-4||SGD DESC:Mitochondrial outer membrane protein required to initiate mitophagy; recruits the autophagy adaptor protein Atg11p and the ubiquitin-like protein Atg8p to the mitochondrial surface to initiate mitophagy, the selective vacuolar degradation of mitochondria in response to starvation; can promote pexophagy when placed ectopically in the peroxisomal membrane Gene:ATP17(YDR377W)|FD-Score:3.12|P-value:8.96E-4||SGD DESC:Subunit f of the F0 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:BSD2(YBR290W)|FD-Score:3.24|P-value:5.89E-4||SGD DESC:Heavy metal ion homeostasis protein, facilitates trafficking of Smf1p and Smf2p metal transporters to the vacuole where they are degraded, controls metal ion transport, prevents metal hyperaccumulation, functions in copper detoxification Gene:CCP1(YKR066C)|FD-Score:3.76|P-value:8.37E-5||SGD DESC:Mitochondrial cytochrome-c peroxidase; degrades reactive oxygen species in mitochondria, involved in the response to oxidative stress Gene:DDC1(YPL194W)|FD-Score:3.18|P-value:7.34E-4||SGD DESC:DNA damage checkpoint protein; part of a PCNA-like complex required for DNA damage response, required for pachytene checkpoint to inhibit cell cycle in response to unrepaired recombination intermediates; potential Cdc28p substrate; forms nuclear foci upon DNA replication stress Gene:EMP47(YFL048C)|FD-Score:4.16|P-value:1.58E-5||SGD DESC:Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport Gene:ERC1(YHR032W)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:Member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily; overproduction confers ethionine resistance and accumulation of S-adenosylmethionine Gene:FSH3(YOR280C)|FD-Score:4.52|P-value:3.13E-6||SGD DESC:Putative serine hydrolase; likely target of Cyc8p-Tup1p-Rfx1p transcriptional regulation; sequence is similar to S. cerevisiae Fsh1p and Fsh2p and the human candidate tumor suppressor OVCA2 Gene:GAS2(YLR343W)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:1,3-beta-glucanosyltransferase, involved with Gas4p in spore wall assembly; has similarity to Gas1p Gene:GCN2(YDR283C)|FD-Score:4.95|P-value:3.68E-7||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN20(YFR009W)|FD-Score:5.48|P-value:2.12E-8||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:5.22|P-value:9.07E-8||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:5.31|P-value:5.47E-8||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GCV3(YAL044C)|FD-Score:-3.4|P-value:3.33E-4||SGD DESC:H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF Gene:GDA1(YEL042W)|FD-Score:3.68|P-value:1.19E-4||SGD DESC:Guanosine diphosphatase located in the Golgi, involved in the transport of GDP-mannose into the Golgi lumen by converting GDP to GMP after mannose is transferred its substrate Gene:GYP1(YOR070C)|FD-Score:4.53|P-value:2.89E-6||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HBN1(YCL026C-B_p)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Protein of unknown function; similar to bacterial nitroreductases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein becomes insoluble upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:HMG1(YML075C)|FD-Score:4.48|P-value:3.69E-6||SGD DESC:HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress Gene:HOC1(YJR075W)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Alpha-1,6-mannosyltransferase involved in cell wall mannan biosynthesis; subunit of a Golgi-localized complex that also contains Anp1p, Mnn9p, Mnn11p, and Mnn10p; identified as a suppressor of a cell lysis sensitive pkc1-371 allele Gene:HRT3(YLR097C)|FD-Score:3.13|P-value:8.74E-4||SGD DESC:Putative SCF-ubiquitin ligase F-box protein, based on both genetic and physical interactions and sequence similarity; identified in association with Cdc53p, Skp1p and Ubi4 in large and small-scale studies Gene:KEL1(YHR158C)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Protein required for proper cell fusion and cell morphology; functions in a complex with Kel2p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate Gene:LAG2(YOL025W)|FD-Score:-3.16|P-value:7.87E-4||SGD DESC:Protein that negatively regulates the SCF E3-ubiquitin ligase by interacting with and preventing neddyation of the cullin subunit, Cdc53p; longevity determinant that is preferentially expressed in young cells; similar to mammalian Cand1 Gene:MNN2(YBR015C)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:NAT5(YOR253W)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:NBP2(YDR162C)|FD-Score:3.14|P-value:8.35E-4||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:NGG1(YDR176W)|FD-Score:3.22|P-value:6.31E-4||SGD DESC:Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex Gene:NST1(YNL091W)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Protein of unknown function, mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1 Gene:NTG2(YOL043C)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair, localizes to the nucleus; sumoylated; NTG2 has a paralog, NTG1, that arose from the whole genome duplication Gene:PDR8(YLR266C)|FD-Score:4.09|P-value:2.12E-5||SGD DESC:Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; PDR8 has a paralog, YRR1, that arose from the whole genome duplication Gene:PMP1(YCR024C-A)|FD-Score:4.87|P-value:5.67E-7||SGD DESC:Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication Gene:POM152(YMR129W)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Glycoprotein subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NUP210 Gene:PUS5(YLR165C)|FD-Score:-3.34|P-value:4.22E-4||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine (Psi)-2819 in mitochondrial 21S rRNA; not essential for viability Gene:RIC1(YLR039C)|FD-Score:4.81|P-value:7.45E-7||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:SAP155(YFR040W)|FD-Score:4.15|P-value:1.66E-5||SGD DESC:Protein required for function of the Sit4p protein phosphatase; forms a complex with Sit4p; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p; protein abundance increases in response to DNA replication stress Gene:SWR1(YDR334W)|FD-Score:-3.89|P-value:5.06E-5||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:TAN1(YGL232W)|FD-Score:-3.9|P-value:4.75E-5||SGD DESC:Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress Gene:TCA17(YEL048C)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Subunit of TRAPPII, a multimeric GEF involved in intra-Golgi and endosome-to-Golgi transport; promotes association of TRAPPII-specific subunits with the core complex; sedlin related; human Sedlin mutations cause SEDT, a skeletal disorder Gene:THI21(YPL258C)|FD-Score:3.92|P-value:4.38E-5||SGD DESC:Hydroxymethylpyrimidine (HMP) and HMP-phosphate kinase; involved in thiamine biosynthesis; member of a gene family with THI20 and THI22; functionally redundant with Thi20p Gene:TNA1(YGR260W)|FD-Score:-4.24|P-value:1.14E-5||SGD DESC:High affinity nicotinic acid plasma membrane permease, responsible for uptake of low levels of nicotinic acid; expression of the gene increases in the absence of extracellular nicotinic acid or para-aminobenzoate (PABA) Gene:TPS2(YDR074W)|FD-Score:-3.2|P-value:6.88E-4||SGD DESC:Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in synthesis of the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress Gene:TRP2(YER090W)|FD-Score:4.75|P-value:9.94E-7||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:6.9|P-value:2.53E-12||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:5.24|P-value:7.96E-8||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:5.97|P-value:1.21E-9||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TUS1(YLR425W)|FD-Score:4.19|P-value:1.39E-5||SGD DESC:Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate Gene:YDR008C(YDR008C_d)|FD-Score:9.62|P-value:3.18E-22||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR149C(YDR149C_d)|FD-Score:4.98|P-value:3.23E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NUM1; null mutation blocks anaerobic growth Gene:YEF1(YEL041W)|FD-Score:-3.27|P-value:5.37E-4||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication Gene:YER158C(YER158C_p)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Protein of unknown function; potentially phosphorylated by Cdc28p; YER158C has a paralog, AFR1, that arose from the whole genome duplication Gene:YGR001C(YGR001C_p)|FD-Score:4.03|P-value:2.78E-5||SGD DESC:Putative protein of unknown function with similarity to methyltransferase family members; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; required for replication of Brome mosaic virus in S. cerevisiae Gene:YIA6(YIL006W)|FD-Score:-3.39|P-value:3.48E-4||SGD DESC:Mitochondrial NAD+ transporter, involved in the transport of NAD+ into the mitochondria (see also YEA6); member of the mitochondrial carrier subfamily; disputed role as a pyruvate transporter; has putative mouse and human orthologs Gene:YKR018C(YKR018C_p)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication Gene:YLR125W(YLR125W_p)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Putative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene Gene:YLR366W(YLR366W_d)|FD-Score:-3.37|P-value:3.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR364C-A Gene:YML009W-B(YML009W-B_d)|FD-Score:-3.17|P-value:7.65E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition Gene:YNL058C(YNL058C_p)|FD-Score:3.21|P-value:6.67E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YNL058C is not an essential gene Gene:YNL193W(YNL193W_p)|FD-Score:6.78|P-value:5.88E-12||SGD DESC:Putative protein of unknown function; exhibits a two-hybrid interaction with Yhr151cp in a large-scale analysis Gene:YOR121C(YOR121C_d)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GCY1/YOR120W Gene:YPL199C(YPL199C_p)|FD-Score:-3.53|P-value:2.04E-4||SGD DESC:Putative protein of unknown function, predicted to be palmitoylated Gene:YPL247C(YPL247C_p)|FD-Score:-3.18|P-value:7.33E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest Gene:YPT6(YLR262C)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:ACM1(YPL267W)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:Pseudosubstrate inhibitor of the anaphase-promoting complex/cyclosome (APC/C), that suppresses APC/C [Cdh1]-mediated proteolysis of mitotic cyclins; associates with Cdh1p, Bmh1p and Bmh2p; cell cycle regulated protein Gene:ALE1(YOR175C)|FD-Score:3.24|P-value:6.04E-4||SGD DESC:Broad-specificity lysophospholipid acyltransferase, part of MBOAT family of membrane-bound O-acyltransferases; key component of Lands cycle; may have role in fatty acid exchange at sn-2 position of mature glycerophospholipids Gene:ARO1(YDR127W)|FD-Score:4.68|P-value:1.46E-6||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO3(YDR035W)|FD-Score:3.89|P-value:5.10E-5||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ATF1(YOR377W)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Alcohol acetyltransferase; responsible for the major part of volatile acetate ester production during fermentation; main enzyme involved in terpenyl acetate synthesis; potential roles in lipid and sterol metabolism Gene:ATG32(YIL146C)|FD-Score:3.12|P-value:9.12E-4||SGD DESC:Mitochondrial outer membrane protein required to initiate mitophagy; recruits the autophagy adaptor protein Atg11p and the ubiquitin-like protein Atg8p to the mitochondrial surface to initiate mitophagy, the selective vacuolar degradation of mitochondria in response to starvation; can promote pexophagy when placed ectopically in the peroxisomal membrane Gene:ATP17(YDR377W)|FD-Score:3.12|P-value:8.96E-4||SGD DESC:Subunit f of the F0 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:BSD2(YBR290W)|FD-Score:3.24|P-value:5.89E-4||SGD DESC:Heavy metal ion homeostasis protein, facilitates trafficking of Smf1p and Smf2p metal transporters to the vacuole where they are degraded, controls metal ion transport, prevents metal hyperaccumulation, functions in copper detoxification Gene:CCP1(YKR066C)|FD-Score:3.76|P-value:8.37E-5||SGD DESC:Mitochondrial cytochrome-c peroxidase; degrades reactive oxygen species in mitochondria, involved in the response to oxidative stress Gene:DDC1(YPL194W)|FD-Score:3.18|P-value:7.34E-4||SGD DESC:DNA damage checkpoint protein; part of a PCNA-like complex required for DNA damage response, required for pachytene checkpoint to inhibit cell cycle in response to unrepaired recombination intermediates; potential Cdc28p substrate; forms nuclear foci upon DNA replication stress Gene:EMP47(YFL048C)|FD-Score:4.16|P-value:1.58E-5||SGD DESC:Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport Gene:ERC1(YHR032W)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:Member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily; overproduction confers ethionine resistance and accumulation of S-adenosylmethionine Gene:FSH3(YOR280C)|FD-Score:4.52|P-value:3.13E-6||SGD DESC:Putative serine hydrolase; likely target of Cyc8p-Tup1p-Rfx1p transcriptional regulation; sequence is similar to S. cerevisiae Fsh1p and Fsh2p and the human candidate tumor suppressor OVCA2 Gene:GAS2(YLR343W)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:1,3-beta-glucanosyltransferase, involved with Gas4p in spore wall assembly; has similarity to Gas1p Gene:GCN2(YDR283C)|FD-Score:4.95|P-value:3.68E-7||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN20(YFR009W)|FD-Score:5.48|P-value:2.12E-8||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:5.22|P-value:9.07E-8||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:5.31|P-value:5.47E-8||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GCV3(YAL044C)|FD-Score:-3.4|P-value:3.33E-4||SGD DESC:H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF Gene:GDA1(YEL042W)|FD-Score:3.68|P-value:1.19E-4||SGD DESC:Guanosine diphosphatase located in the Golgi, involved in the transport of GDP-mannose into the Golgi lumen by converting GDP to GMP after mannose is transferred its substrate Gene:GYP1(YOR070C)|FD-Score:4.53|P-value:2.89E-6||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HBN1(YCL026C-B_p)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Protein of unknown function; similar to bacterial nitroreductases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein becomes insoluble upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:HMG1(YML075C)|FD-Score:4.48|P-value:3.69E-6||SGD DESC:HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress Gene:HOC1(YJR075W)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Alpha-1,6-mannosyltransferase involved in cell wall mannan biosynthesis; subunit of a Golgi-localized complex that also contains Anp1p, Mnn9p, Mnn11p, and Mnn10p; identified as a suppressor of a cell lysis sensitive pkc1-371 allele Gene:HRT3(YLR097C)|FD-Score:3.13|P-value:8.74E-4||SGD DESC:Putative SCF-ubiquitin ligase F-box protein, based on both genetic and physical interactions and sequence similarity; identified in association with Cdc53p, Skp1p and Ubi4 in large and small-scale studies Gene:KEL1(YHR158C)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Protein required for proper cell fusion and cell morphology; functions in a complex with Kel2p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate Gene:LAG2(YOL025W)|FD-Score:-3.16|P-value:7.87E-4||SGD DESC:Protein that negatively regulates the SCF E3-ubiquitin ligase by interacting with and preventing neddyation of the cullin subunit, Cdc53p; longevity determinant that is preferentially expressed in young cells; similar to mammalian Cand1 Gene:MNN2(YBR015C)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:NAT5(YOR253W)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:NBP2(YDR162C)|FD-Score:3.14|P-value:8.35E-4||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:NGG1(YDR176W)|FD-Score:3.22|P-value:6.31E-4||SGD DESC:Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex Gene:NST1(YNL091W)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Protein of unknown function, mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1 Gene:NTG2(YOL043C)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair, localizes to the nucleus; sumoylated; NTG2 has a paralog, NTG1, that arose from the whole genome duplication Gene:PDR8(YLR266C)|FD-Score:4.09|P-value:2.12E-5||SGD DESC:Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; PDR8 has a paralog, YRR1, that arose from the whole genome duplication Gene:PMP1(YCR024C-A)|FD-Score:4.87|P-value:5.67E-7||SGD DESC:Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication Gene:POM152(YMR129W)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Glycoprotein subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NUP210 Gene:PUS5(YLR165C)|FD-Score:-3.34|P-value:4.22E-4||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine (Psi)-2819 in mitochondrial 21S rRNA; not essential for viability Gene:RIC1(YLR039C)|FD-Score:4.81|P-value:7.45E-7||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:SAP155(YFR040W)|FD-Score:4.15|P-value:1.66E-5||SGD DESC:Protein required for function of the Sit4p protein phosphatase; forms a complex with Sit4p; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p; protein abundance increases in response to DNA replication stress Gene:SWR1(YDR334W)|FD-Score:-3.89|P-value:5.06E-5||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:TAN1(YGL232W)|FD-Score:-3.9|P-value:4.75E-5||SGD DESC:Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress Gene:TCA17(YEL048C)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Subunit of TRAPPII, a multimeric GEF involved in intra-Golgi and endosome-to-Golgi transport; promotes association of TRAPPII-specific subunits with the core complex; sedlin related; human Sedlin mutations cause SEDT, a skeletal disorder Gene:THI21(YPL258C)|FD-Score:3.92|P-value:4.38E-5||SGD DESC:Hydroxymethylpyrimidine (HMP) and HMP-phosphate kinase; involved in thiamine biosynthesis; member of a gene family with THI20 and THI22; functionally redundant with Thi20p Gene:TNA1(YGR260W)|FD-Score:-4.24|P-value:1.14E-5||SGD DESC:High affinity nicotinic acid plasma membrane permease, responsible for uptake of low levels of nicotinic acid; expression of the gene increases in the absence of extracellular nicotinic acid or para-aminobenzoate (PABA) Gene:TPS2(YDR074W)|FD-Score:-3.2|P-value:6.88E-4||SGD DESC:Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in synthesis of the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress Gene:TRP2(YER090W)|FD-Score:4.75|P-value:9.94E-7||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:6.9|P-value:2.53E-12||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:5.24|P-value:7.96E-8||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:5.97|P-value:1.21E-9||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TUS1(YLR425W)|FD-Score:4.19|P-value:1.39E-5||SGD DESC:Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate Gene:YDR008C(YDR008C_d)|FD-Score:9.62|P-value:3.18E-22||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR149C(YDR149C_d)|FD-Score:4.98|P-value:3.23E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NUM1; null mutation blocks anaerobic growth Gene:YEF1(YEL041W)|FD-Score:-3.27|P-value:5.37E-4||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication Gene:YER158C(YER158C_p)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Protein of unknown function; potentially phosphorylated by Cdc28p; YER158C has a paralog, AFR1, that arose from the whole genome duplication Gene:YGR001C(YGR001C_p)|FD-Score:4.03|P-value:2.78E-5||SGD DESC:Putative protein of unknown function with similarity to methyltransferase family members; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; required for replication of Brome mosaic virus in S. cerevisiae Gene:YIA6(YIL006W)|FD-Score:-3.39|P-value:3.48E-4||SGD DESC:Mitochondrial NAD+ transporter, involved in the transport of NAD+ into the mitochondria (see also YEA6); member of the mitochondrial carrier subfamily; disputed role as a pyruvate transporter; has putative mouse and human orthologs Gene:YKR018C(YKR018C_p)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication Gene:YLR125W(YLR125W_p)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Putative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene Gene:YLR366W(YLR366W_d)|FD-Score:-3.37|P-value:3.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR364C-A Gene:YML009W-B(YML009W-B_d)|FD-Score:-3.17|P-value:7.65E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition Gene:YNL058C(YNL058C_p)|FD-Score:3.21|P-value:6.67E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YNL058C is not an essential gene Gene:YNL193W(YNL193W_p)|FD-Score:6.78|P-value:5.88E-12||SGD DESC:Putative protein of unknown function; exhibits a two-hybrid interaction with Yhr151cp in a large-scale analysis Gene:YOR121C(YOR121C_d)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GCY1/YOR120W Gene:YPL199C(YPL199C_p)|FD-Score:-3.53|P-value:2.04E-4||SGD DESC:Putative protein of unknown function, predicted to be palmitoylated Gene:YPL247C(YPL247C_p)|FD-Score:-3.18|P-value:7.33E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest Gene:YPT6(YLR262C)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR526C_d3.739.41E-50.31YDR526C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YOR181W3.601.59E-40.31LAS17Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP)
YPL151C3.413.23E-40.31PRP46Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs
YMR213W3.373.71E-40.31CEF1Essential splicing factor; associated with Prp19p and the spliceosome, contains an N-terminal c-Myb DNA binding motif necessary for cell viability but not for Prp19p association, evolutionarily conserved and homologous to S. pombe Cdc5p
YPL075W3.070.001080.11GCR1Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p
YDR228C2.960.001550.06PCF11mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping
YFL039C2.900.001850.02ACT1Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions
YLR066W2.880.001970.01SPC3Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23
YPR175W2.880.002010.03DPB2Second largest subunit of DNA polymerase II (DNA polymerase epsilon), required for maintenance of fidelity of chromosomal replication; expression peaks at the G1/S phase boundary; Cdc28p substrate
YMR149W2.850.002190.04SWP1Delta subunit of the oligosaccharyl transferase glycoprotein complex, which is required for N-linked glycosylation of proteins in the endoplasmic reticulum
YBR160W2.810.002510.04CDC28Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with G1 cyclins (CLNs) and G2/M cyclins (CLBs) which direct the CDK to specific substrates; involved in modulating membrane trafficking dynamics; protein abundance increases in response to DNA replication stress
YGR090W2.770.002840.13UTP22Component of the small-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals
YLR029C2.640.004190.02RPL15ARibosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication
YOR057W2.620.004390.07SGT1Cochaperone protein; regulates activity of adenylyl cyclase Cyr1p; involved in kinetochore complex assembly; associates with the SCF (Skp1p/Cdc53p/F box protein) ubiquitin ligase complex; acts as a linker between Skp1p and HSP90 complexes; protein abundance increases in response to DNA replication stress
YNR035C2.550.005310.05ARC35Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR008C_d9.623.18E-22YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YKL211C6.902.53E-12TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YNL193W_p6.785.88E-12YNL193W_pPutative protein of unknown function; exhibits a two-hybrid interaction with Yhr151cp in a large-scale analysis
YGL026C5.971.21E-9TRP5Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis
YFR009W5.482.12E-8GCN20Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA
YEL009C5.315.47E-8GCN4Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
YDR354W5.247.96E-8TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YKR026C5.229.07E-8GCN3Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression
YDR149C_d4.983.23E-7YDR149C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NUM1; null mutation blocks anaerobic growth
YDR283C4.953.68E-7GCN2Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control
YCR024C-A4.875.67E-7PMP1Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication
YLR039C4.817.45E-7RIC1Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes
YER090W4.759.94E-7TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YDR127W4.681.46E-6ARO1Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
YOR070C4.532.89E-6GYP1Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion

GO enrichment analysis for SGTC_2678
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.4117.31E-240SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.306452
0.3641.05E-184SGTC_511013-0126 23.4 μMChemDiv (Drug-like library)16278500.0632911
0.3639.00E-184SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.107143
0.3561.28E-175SGTC_1697st025554 27.7 μMTimTec (Natural product derivative library)12695190.0985916
0.3561.33E-175SGTC_1801486-1293 102.8 μMChemDiv (Drug-like library)41405360.0705882
0.3543.08E-174SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.306452
0.3523.95E-172SGTC_18825615643 20.0 μMMiscellaneous22530750.0813954TRP & mitochondrial translation
0.3515.50E-171SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.0483871
0.3517.50E-171SGTC_8191013-0258 27.7 μMChemDiv (Drug-like library)57531980.0714286
0.3511.89E-170SGTC_6770929-0063 136.0 μMChemDiv (Drug-like library)15395830.1
0.3339.09E-153SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.129412
0.3311.40E-150SGTC_1572epiandrosterone 68.9 μMTimTec (Pure natural product library)4413020.265625
0.3302.21E-150SGTC_18835-benzylidenerhodanine 20.0 μMMiscellaneous12732100.0757576TRP & mitochondrial translation
0.3282.69E-148SGTC_33089128332 17.6 μMChembridge (Drug-like library)173328050.0487805plasma membrane duress
0.3273.70E-147SGTC_1649st011737 51.3 μMTimTec (Natural product derivative library)17610350.0493827

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_406estrone100 μM0.55870Miscellaneous270.366083.93812
SGTC_1519esterone74 μM0.5698TimTec (Pure natural product library)270.366083.93812DNA intercalators
SGTC_1767st04499270.3 μM0.3593759600693TimTec (Natural product derivative library)284.395963.50123
SGTC_231b-estradiol524 μM0.33871450Miscellaneous272.381963.83822
SGTC_2650prasterone100 μM0.30645216759247Microsource (Natural product library)288.424423.33812
SGTC_2758dehydroepiandrosterone69.35 μM0.3064525881Miscellaneous288.424423.33812
SGTC_1587estradiol valerate56.1 μM0.28947413791TimTec (Pure natural product library)356.498385.79613
SGTC_416estradiol valerate100 μM0.28947413791TimTec (Pure natural product library)356.498385.79613
SGTC_1572epiandrosterone68.9 μM0.265625441302TimTec (Pure natural product library)290.44033.58812
SGTC_2540epiandrosterone100 μM0.265625441302TimTec (Pure natural product library)290.44033.58812
SGTC_2584androsterone48.74 μM0.2656252754130Microsource (Natural product library)290.44033.58812cell wall
SGTC_1745st08149574 μM0.26153813842402TimTec (Natural product derivative library)270.279962.94814RNA processing & uracil transport