rapamycin FDA approved compound


Established Yeast Drug Target : TOR1 TOR2

Rapamycin is an potent immunosuppressive and anticancer drug. It forms a complex with FKBP12 that binds to and inhibits the molecular target of rapamycin (mTOR). mTOR is a member of the phosphoinositide kinase-related kinase (PIKK) family that enhances cellular proliferation via the phosphoinositol 3-kinase/Akt signaling pathway. Inhibition of this pathway by rapamycin blocks downstream elements that result in cell cycle arrest in G1. that acts by inhibiting TOR. TOR (target of rapamycin) is a phosphatidylinositol kinase-related protein kinase that controls cell growth in response to nutrients.

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PubChem MeSH terms: Anti-Bacterial Agents;Antibiotics, Antineoplastic;Antifungal Agents;Immunosuppressive Agents



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2679
Screen concentration 1.0 nM
Source Microsource (Natural product library)
PubChem CID 5284616
SMILES CC1CCC2CC(C(=CC=CC=CC(CC(C(=O)C(C(C(=CC(C(=O)CC(OC(=O)C3CCCCN3C(=O)C(=O)C1(O2)O)C(C)CC4CCC(C(C4)OC)O)C)C)O)OC)C)C)C)OC
Standardized SMILES COC1CC(CC(C)C2CC(=O)C(C)C=C(C)C(O)C(OC)C(=O)C(C)CC(C)C=CC=CC=C(C)C(CC3CCC(C)C(O)(O3)C(=O)C(=O)N4CCCCC4C(=O)O2)OC)CCC1O
Molecular weight 914.1719
ALogP 6.3
H-bond donor count 3
H-bond acceptor count 13
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 74.85
% growth inhibition (Hom. pool) 49.59


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5284616
Download HIP data (tab-delimited text)  (excel)
Gene:ACC1(YNR016C)|FD-Score:6.21|P-value:2.61E-10|Clearance:0.53||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ACT1(YFL039C)|FD-Score:6.82|P-value:4.69E-12|Clearance:0.02||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:ARP7(YPR034W)|FD-Score:9.06|P-value:6.28E-20|Clearance:0.28||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:ARP9(YMR033W)|FD-Score:5.22|P-value:9.19E-8|Clearance:0.09||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:BRL1(YHR036W)|FD-Score:15.1|P-value:6.56E-52|Clearance:3.45||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:CCT3(YJL014W)|FD-Score:-5.97|P-value:1.19E-9|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT4(YDL143W)|FD-Score:-3.55|P-value:1.90E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT6(YDR188W)|FD-Score:-3.19|P-value:7.22E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif Gene:CCT7(YJL111W)|FD-Score:-4.37|P-value:6.17E-6|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CDC12(YHR107C)|FD-Score:7.45|P-value:4.73E-14|Clearance:0.63||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CDC20(YGL116W)|FD-Score:11.7|P-value:1.12E-31|Clearance:0.1||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CDC31(YOR257W)|FD-Score:11.3|P-value:6.10E-30|Clearance:0.8||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:CDC39(YCR093W)|FD-Score:5.69|P-value:6.48E-9|Clearance:0.38||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:CDC60(YPL160W)|FD-Score:3.41|P-value:3.23E-4|Clearance:0.04||SGD DESC:Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA Gene:CMD1(YBR109C)|FD-Score:-4.14|P-value:1.74E-5|Clearance:0||SGD DESC:Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin Gene:COP1(YDL145C)|FD-Score:-3.55|P-value:1.92E-4|Clearance:0||SGD DESC:Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway Gene:CRM1(YGR218W)|FD-Score:-3.24|P-value:6.05E-4|Clearance:0||SGD DESC:Major karyopherin, involved in export of proteins, RNAs, and ribosomal subunits from the nucleus; exportin Gene:CSE1(YGL238W)|FD-Score:4.31|P-value:8.28E-6|Clearance:0.11||SGD DESC:Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation Gene:DFR1(YOR236W)|FD-Score:4.05|P-value:2.57E-5|Clearance:0.03||SGD DESC:Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism Gene:EPL1(YFL024C)|FD-Score:7.87|P-value:1.72E-15|Clearance:0.43||SGD DESC:Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb Gene:ERD2(YBL040C)|FD-Score:-3.57|P-value:1.82E-4|Clearance:0||SGD DESC:HDEL receptor, an integral membrane protein that binds to the HDEL motif in proteins destined for retention in the endoplasmic reticulum; has a role in maintenance of normal levels of ER-resident proteins Gene:ERG1(YGR175C)|FD-Score:-3.47|P-value:2.58E-4|Clearance:0||SGD DESC:Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine Gene:ERG11(YHR007C)|FD-Score:7.94|P-value:9.90E-16|Clearance:0.03||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:GAA1(YLR088W)|FD-Score:-4.09|P-value:2.11E-5|Clearance:0||SGD DESC:Subunit of the GPI (glycosylphosphatidylinositol):protein transamidase complex, removes the GPI-anchoring signal and attaches GPI to proteins in the ER Gene:GCD10(YNL062C)|FD-Score:5.01|P-value:2.74E-7|Clearance:0.02||SGD DESC:Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression Gene:GCD11(YER025W)|FD-Score:5.31|P-value:5.49E-8|Clearance:0.05||SGD DESC:Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met Gene:GCD2(YGR083C)|FD-Score:3.87|P-value:5.38E-5|Clearance:0.2||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GCD7(YLR291C)|FD-Score:5.26|P-value:7.37E-8|Clearance:0.02||SGD DESC:Beta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GCR1(YPL075W)|FD-Score:4.84|P-value:6.47E-7|Clearance:0||SGD DESC:Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p Gene:GLC7(YER133W)|FD-Score:9.46|P-value:1.58E-21|Clearance:0.16||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GPI14(YJR013W)|FD-Score:8.78|P-value:8.07E-19|Clearance:0.82||SGD DESC:Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M Gene:IFH1(YLR223C)|FD-Score:3.27|P-value:5.39E-4|Clearance:0||SGD DESC:Coactivator that regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing Gene:KAP95(YLR347C)|FD-Score:4.84|P-value:6.56E-7|Clearance:0.01||SGD DESC:Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p Gene:KAR2(YJL034W)|FD-Score:-3.33|P-value:4.28E-4|Clearance:0||SGD DESC:ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p Gene:KOG1(YHR186C)|FD-Score:16.9|P-value:4.41E-64|Clearance:3.45||SGD DESC:Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors Gene:LCB2(YDR062W)|FD-Score:-3.55|P-value:1.96E-4|Clearance:0||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:LSM2(YBL026W)|FD-Score:3.27|P-value:5.44E-4|Clearance:0.03||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MAS1(YLR163C)|FD-Score:-3.63|P-value:1.42E-4|Clearance:0||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:MCD4(YKL165C)|FD-Score:-3.16|P-value:7.80E-4|Clearance:0||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:MED4(YOR174W)|FD-Score:-5.42|P-value:2.96E-8|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:NEO1(YIL048W)|FD-Score:11.6|P-value:3.55E-31|Clearance:0.25||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:NOP56(YLR197W)|FD-Score:-3.18|P-value:7.32E-4|Clearance:0||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:NOP8(YOL144W)|FD-Score:4.79|P-value:8.50E-7|Clearance:0.27||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:PKC1(YBL105C)|FD-Score:-7.95|P-value:9.68E-16|Clearance:0||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:POL12(YBL035C)|FD-Score:-3.6|P-value:1.60E-4|Clearance:0||SGD DESC:B subunit of DNA polymerase alpha-primase complex, required for initiation of DNA replication during mitotic and premeiotic DNA synthesis; also functions in telomere capping and length regulation Gene:PRE1(YER012W)|FD-Score:4.14|P-value:1.74E-5|Clearance:0.03||SGD DESC:Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle Gene:PRE2(YPR103W)|FD-Score:5.24|P-value:8.05E-8|Clearance:0.02||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:PRE4(YFR050C)|FD-Score:6.6|P-value:2.04E-11|Clearance:0.01||SGD DESC:Beta 7 subunit of the 20S proteasome Gene:PRE5(YMR314W)|FD-Score:3.56|P-value:1.88E-4|Clearance:0.03||SGD DESC:Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PRE7(YBL041W)|FD-Score:5.13|P-value:1.48E-7|Clearance:0.1||SGD DESC:Beta 6 subunit of the 20S proteasome Gene:PRE8(YML092C)|FD-Score:4.51|P-value:3.20E-6|Clearance:0.06||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:PSE1(YMR308C)|FD-Score:-3.59|P-value:1.68E-4|Clearance:0||SGD DESC:Karyopherin/importin that interacts with the nuclear pore complex; acts as the nuclear import receptor for specific proteins, including Pdr1p, Yap1p, Ste12p, and Aft1p Gene:PUP1(YOR157C)|FD-Score:3.41|P-value:3.21E-4|Clearance:0||SGD DESC:Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z Gene:REB1(YBR049C)|FD-Score:7.96|P-value:8.48E-16|Clearance:0.02||SGD DESC:RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication Gene:RET2(YFR051C)|FD-Score:-4.87|P-value:5.53E-7|Clearance:0||SGD DESC:Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RET3(YPL010W)|FD-Score:-3.2|P-value:6.96E-4|Clearance:0||SGD DESC:Zeta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RPB5(YBR154C)|FD-Score:5.03|P-value:2.47E-7|Clearance:0.02||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:RPL25(YOL127W)|FD-Score:6.8|P-value:5.26E-12|Clearance:0.12||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPL32(YBL092W)|FD-Score:4.45|P-value:4.27E-6|Clearance:0.03||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPL5(YPL131W)|FD-Score:3.51|P-value:2.27E-4|Clearance:0.09||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:RPN5(YDL147W)|FD-Score:6.4|P-value:7.57E-11|Clearance:0.09||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RPS13(YDR064W)|FD-Score:3.23|P-value:6.29E-4|Clearance:0.02||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:RPS15(YOL040C)|FD-Score:3.21|P-value:6.73E-4|Clearance:0.13||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RSC58(YLR033W)|FD-Score:8.78|P-value:7.89E-19|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC6(YCR052W)|FD-Score:3.24|P-value:5.96E-4|Clearance:0.02||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:RSC8(YFR037C)|FD-Score:4.99|P-value:3.00E-7|Clearance:0.11||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RSC9(YML127W)|FD-Score:4.82|P-value:7.04E-7|Clearance:0.01||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:RSP5(YER125W)|FD-Score:-4.35|P-value:6.90E-6|Clearance:0||SGD DESC:E3 ubiquitin ligase of the NEDD4 family; involved in regulating many cellular processes including MVB sorting, heat shock response, transcription, endocytosis, and ribosome stability; human homolog is involved in Liddle syndrome; mutant tolerates aneuploidy; ubiquitylates Sec23p Gene:RVB2(YPL235W)|FD-Score:4.42|P-value:4.97E-6|Clearance:0.11||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SEC21(YNL287W)|FD-Score:-3.94|P-value:4.09E-5|Clearance:0||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:SEC26(YDR238C)|FD-Score:-4.5|P-value:3.37E-6|Clearance:0||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SEC27(YGL137W)|FD-Score:-4.87|P-value:5.61E-7|Clearance:0||SGD DESC:Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP Gene:SES1(YDR023W)|FD-Score:9.62|P-value:3.28E-22|Clearance:0.16||SGD DESC:Cytosolic seryl-tRNA synthetase, class II aminoacyl-tRNA synthetase that aminoacylates tRNA(Ser), displays tRNA-dependent amino acid recognition which enhances discrimination of the serine substrate, interacts with peroxin Pex21p Gene:SGV1(YPR161C)|FD-Score:3.92|P-value:4.51E-5|Clearance:0.04||SGD DESC:Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins Gene:SIS1(YNL007C)|FD-Score:3.53|P-value:2.12E-4|Clearance:0.02||SGD DESC:Type II HSP40 co-chaperone that interacts with the HSP70 protein Ssa1p; not functionally redundant with Ydj1p due to due to substrate specificity; has similarity to bacterial DnaJ proteins; protein abundance increases in response to DNA replication stress Gene:SPB4(YFL002C)|FD-Score:3.63|P-value:1.42E-4|Clearance:0.07||SGD DESC:Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients Gene:SPT14(YPL175W)|FD-Score:4.81|P-value:7.52E-7|Clearance:0.02||SGD DESC:UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins Gene:SRB4(YER022W)|FD-Score:-3.46|P-value:2.72E-4|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination Gene:SRB7(YDR308C)|FD-Score:-5.25|P-value:7.73E-8|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; target of the global repressor Tup1p Gene:SSY5(YJL156C)|FD-Score:11.7|P-value:9.35E-32|Clearance:0.02||SGD DESC:Serine protease of SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); contains an inhibitory domain that dissociates in response to extracellular amino acids, freeing a catalytic domain to activate transcription factor Stp1p Gene:SUI3(YPL237W)|FD-Score:9.29|P-value:7.47E-21|Clearance:0.23||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:TCP1(YDR212W)|FD-Score:-3.55|P-value:1.94E-4|Clearance:0||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:TFA1(YKL028W)|FD-Score:4.01|P-value:2.97E-5|Clearance:0||SGD DESC:TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening Gene:TIF35(YDR429C)|FD-Score:-3.74|P-value:9.09E-5|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TIM12(YBR091C)|FD-Score:7.91|P-value:1.31E-15|Clearance:0.03||SGD DESC:Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins Gene:TIM54(YJL054W)|FD-Score:10.5|P-value:4.13E-26|Clearance:0.88||SGD DESC:Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane Gene:TOM40(YMR203W)|FD-Score:-3.51|P-value:2.25E-4|Clearance:0||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:TOR2(YKL203C)|FD-Score:17.9|P-value:6.33E-72|Clearance:3.45||SGD DESC:PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication Gene:TRE2(YOR256C)|FD-Score:4.88|P-value:5.27E-7|Clearance:0.04||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication Gene:TRM5(YHR070W)|FD-Score:6.59|P-value:2.23E-11|Clearance:0.12||SGD DESC:tRNA(m(1)G37)methyltransferase, methylates a tRNA base adjacent to the anticodon that has a role in prevention of frameshifting; highly conserved across Archaea, Bacteria, and Eukarya Gene:TRS20(YBR254C)|FD-Score:4.11|P-value:2.00E-5|Clearance:0.06||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; mutations in the human homolog cause the spondyloepiphyseal dysplasia tarda (SEDL) disorder Gene:TRS31(YDR472W)|FD-Score:6.32|P-value:1.33E-10|Clearance:0.1||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:TUB1(YML085C)|FD-Score:4.19|P-value:1.38E-5|Clearance:0.05||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:TUB2(YFL037W)|FD-Score:-3.88|P-value:5.20E-5|Clearance:0||SGD DESC:Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria Gene:UTP15(YMR093W)|FD-Score:3.67|P-value:1.21E-4|Clearance:0.04||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:UTP18(YJL069C)|FD-Score:3.37|P-value:3.71E-4|Clearance:0.1||SGD DESC:Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data Gene:UTP22(YGR090W)|FD-Score:-4.66|P-value:1.57E-6|Clearance:0||SGD DESC:Component of the small-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals Gene:UTP9(YHR196W)|FD-Score:3.38|P-value:3.68E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YDL196W(YDL196W_d)|FD-Score:-5.22|P-value:8.98E-8|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YDR187C(YDR187C_d)|FD-Score:-3.47|P-value:2.59E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YDR526C(YDR526C_d)|FD-Score:-4.18|P-value:1.48E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR114C(YGR114C_d)|FD-Score:-4.55|P-value:2.69E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YGR115C(YGR115C_d)|FD-Score:6.47|P-value:5.02E-11|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YOR203W(YOR203W_d)|FD-Score:-3.55|P-value:1.94E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps 5' end of essential DED1 gene required for translation initiation Gene:YPL238C(YPL238C_d)|FD-Score:4.01|P-value:3.02E-5|Clearance:0.1||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Gene:YPT1(YFL038C)|FD-Score:6.68|P-value:1.19E-11|Clearance:0.08||SGD DESC:Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p) Gene:YTH1(YPR107C)|FD-Score:-5.15|P-value:1.31E-7|Clearance:0||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:ACC1(YNR016C)|FD-Score:6.21|P-value:2.61E-10|Clearance:0.53||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ACT1(YFL039C)|FD-Score:6.82|P-value:4.69E-12|Clearance:0.02||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:ARP7(YPR034W)|FD-Score:9.06|P-value:6.28E-20|Clearance:0.28||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:ARP9(YMR033W)|FD-Score:5.22|P-value:9.19E-8|Clearance:0.09||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:BRL1(YHR036W)|FD-Score:15.1|P-value:6.56E-52|Clearance:3.45||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:CCT3(YJL014W)|FD-Score:-5.97|P-value:1.19E-9|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT4(YDL143W)|FD-Score:-3.55|P-value:1.90E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT6(YDR188W)|FD-Score:-3.19|P-value:7.22E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif Gene:CCT7(YJL111W)|FD-Score:-4.37|P-value:6.17E-6|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CDC12(YHR107C)|FD-Score:7.45|P-value:4.73E-14|Clearance:0.63||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CDC20(YGL116W)|FD-Score:11.7|P-value:1.12E-31|Clearance:0.1||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CDC31(YOR257W)|FD-Score:11.3|P-value:6.10E-30|Clearance:0.8||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:CDC39(YCR093W)|FD-Score:5.69|P-value:6.48E-9|Clearance:0.38||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:CDC60(YPL160W)|FD-Score:3.41|P-value:3.23E-4|Clearance:0.04||SGD DESC:Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA Gene:CMD1(YBR109C)|FD-Score:-4.14|P-value:1.74E-5|Clearance:0||SGD DESC:Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin Gene:COP1(YDL145C)|FD-Score:-3.55|P-value:1.92E-4|Clearance:0||SGD DESC:Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway Gene:CRM1(YGR218W)|FD-Score:-3.24|P-value:6.05E-4|Clearance:0||SGD DESC:Major karyopherin, involved in export of proteins, RNAs, and ribosomal subunits from the nucleus; exportin Gene:CSE1(YGL238W)|FD-Score:4.31|P-value:8.28E-6|Clearance:0.11||SGD DESC:Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation Gene:DFR1(YOR236W)|FD-Score:4.05|P-value:2.57E-5|Clearance:0.03||SGD DESC:Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism Gene:EPL1(YFL024C)|FD-Score:7.87|P-value:1.72E-15|Clearance:0.43||SGD DESC:Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb Gene:ERD2(YBL040C)|FD-Score:-3.57|P-value:1.82E-4|Clearance:0||SGD DESC:HDEL receptor, an integral membrane protein that binds to the HDEL motif in proteins destined for retention in the endoplasmic reticulum; has a role in maintenance of normal levels of ER-resident proteins Gene:ERG1(YGR175C)|FD-Score:-3.47|P-value:2.58E-4|Clearance:0||SGD DESC:Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine Gene:ERG11(YHR007C)|FD-Score:7.94|P-value:9.90E-16|Clearance:0.03||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:GAA1(YLR088W)|FD-Score:-4.09|P-value:2.11E-5|Clearance:0||SGD DESC:Subunit of the GPI (glycosylphosphatidylinositol):protein transamidase complex, removes the GPI-anchoring signal and attaches GPI to proteins in the ER Gene:GCD10(YNL062C)|FD-Score:5.01|P-value:2.74E-7|Clearance:0.02||SGD DESC:Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression Gene:GCD11(YER025W)|FD-Score:5.31|P-value:5.49E-8|Clearance:0.05||SGD DESC:Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met Gene:GCD2(YGR083C)|FD-Score:3.87|P-value:5.38E-5|Clearance:0.2||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GCD7(YLR291C)|FD-Score:5.26|P-value:7.37E-8|Clearance:0.02||SGD DESC:Beta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GCR1(YPL075W)|FD-Score:4.84|P-value:6.47E-7|Clearance:0||SGD DESC:Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p Gene:GLC7(YER133W)|FD-Score:9.46|P-value:1.58E-21|Clearance:0.16||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GPI14(YJR013W)|FD-Score:8.78|P-value:8.07E-19|Clearance:0.82||SGD DESC:Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M Gene:IFH1(YLR223C)|FD-Score:3.27|P-value:5.39E-4|Clearance:0||SGD DESC:Coactivator that regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing Gene:KAP95(YLR347C)|FD-Score:4.84|P-value:6.56E-7|Clearance:0.01||SGD DESC:Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p Gene:KAR2(YJL034W)|FD-Score:-3.33|P-value:4.28E-4|Clearance:0||SGD DESC:ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p Gene:KOG1(YHR186C)|FD-Score:16.9|P-value:4.41E-64|Clearance:3.45||SGD DESC:Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors Gene:LCB2(YDR062W)|FD-Score:-3.55|P-value:1.96E-4|Clearance:0||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:LSM2(YBL026W)|FD-Score:3.27|P-value:5.44E-4|Clearance:0.03||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MAS1(YLR163C)|FD-Score:-3.63|P-value:1.42E-4|Clearance:0||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:MCD4(YKL165C)|FD-Score:-3.16|P-value:7.80E-4|Clearance:0||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:MED4(YOR174W)|FD-Score:-5.42|P-value:2.96E-8|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:NEO1(YIL048W)|FD-Score:11.6|P-value:3.55E-31|Clearance:0.25||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:NOP56(YLR197W)|FD-Score:-3.18|P-value:7.32E-4|Clearance:0||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:NOP8(YOL144W)|FD-Score:4.79|P-value:8.50E-7|Clearance:0.27||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:PKC1(YBL105C)|FD-Score:-7.95|P-value:9.68E-16|Clearance:0||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:POL12(YBL035C)|FD-Score:-3.6|P-value:1.60E-4|Clearance:0||SGD DESC:B subunit of DNA polymerase alpha-primase complex, required for initiation of DNA replication during mitotic and premeiotic DNA synthesis; also functions in telomere capping and length regulation Gene:PRE1(YER012W)|FD-Score:4.14|P-value:1.74E-5|Clearance:0.03||SGD DESC:Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle Gene:PRE2(YPR103W)|FD-Score:5.24|P-value:8.05E-8|Clearance:0.02||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:PRE4(YFR050C)|FD-Score:6.6|P-value:2.04E-11|Clearance:0.01||SGD DESC:Beta 7 subunit of the 20S proteasome Gene:PRE5(YMR314W)|FD-Score:3.56|P-value:1.88E-4|Clearance:0.03||SGD DESC:Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PRE7(YBL041W)|FD-Score:5.13|P-value:1.48E-7|Clearance:0.1||SGD DESC:Beta 6 subunit of the 20S proteasome Gene:PRE8(YML092C)|FD-Score:4.51|P-value:3.20E-6|Clearance:0.06||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:PSE1(YMR308C)|FD-Score:-3.59|P-value:1.68E-4|Clearance:0||SGD DESC:Karyopherin/importin that interacts with the nuclear pore complex; acts as the nuclear import receptor for specific proteins, including Pdr1p, Yap1p, Ste12p, and Aft1p Gene:PUP1(YOR157C)|FD-Score:3.41|P-value:3.21E-4|Clearance:0||SGD DESC:Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z Gene:REB1(YBR049C)|FD-Score:7.96|P-value:8.48E-16|Clearance:0.02||SGD DESC:RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication Gene:RET2(YFR051C)|FD-Score:-4.87|P-value:5.53E-7|Clearance:0||SGD DESC:Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RET3(YPL010W)|FD-Score:-3.2|P-value:6.96E-4|Clearance:0||SGD DESC:Zeta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RPB5(YBR154C)|FD-Score:5.03|P-value:2.47E-7|Clearance:0.02||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:RPL25(YOL127W)|FD-Score:6.8|P-value:5.26E-12|Clearance:0.12||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPL32(YBL092W)|FD-Score:4.45|P-value:4.27E-6|Clearance:0.03||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPL5(YPL131W)|FD-Score:3.51|P-value:2.27E-4|Clearance:0.09||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:RPN5(YDL147W)|FD-Score:6.4|P-value:7.57E-11|Clearance:0.09||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RPS13(YDR064W)|FD-Score:3.23|P-value:6.29E-4|Clearance:0.02||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:RPS15(YOL040C)|FD-Score:3.21|P-value:6.73E-4|Clearance:0.13||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RSC58(YLR033W)|FD-Score:8.78|P-value:7.89E-19|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC6(YCR052W)|FD-Score:3.24|P-value:5.96E-4|Clearance:0.02||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:RSC8(YFR037C)|FD-Score:4.99|P-value:3.00E-7|Clearance:0.11||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RSC9(YML127W)|FD-Score:4.82|P-value:7.04E-7|Clearance:0.01||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:RSP5(YER125W)|FD-Score:-4.35|P-value:6.90E-6|Clearance:0||SGD DESC:E3 ubiquitin ligase of the NEDD4 family; involved in regulating many cellular processes including MVB sorting, heat shock response, transcription, endocytosis, and ribosome stability; human homolog is involved in Liddle syndrome; mutant tolerates aneuploidy; ubiquitylates Sec23p Gene:RVB2(YPL235W)|FD-Score:4.42|P-value:4.97E-6|Clearance:0.11||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SEC21(YNL287W)|FD-Score:-3.94|P-value:4.09E-5|Clearance:0||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:SEC26(YDR238C)|FD-Score:-4.5|P-value:3.37E-6|Clearance:0||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SEC27(YGL137W)|FD-Score:-4.87|P-value:5.61E-7|Clearance:0||SGD DESC:Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP Gene:SES1(YDR023W)|FD-Score:9.62|P-value:3.28E-22|Clearance:0.16||SGD DESC:Cytosolic seryl-tRNA synthetase, class II aminoacyl-tRNA synthetase that aminoacylates tRNA(Ser), displays tRNA-dependent amino acid recognition which enhances discrimination of the serine substrate, interacts with peroxin Pex21p Gene:SGV1(YPR161C)|FD-Score:3.92|P-value:4.51E-5|Clearance:0.04||SGD DESC:Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins Gene:SIS1(YNL007C)|FD-Score:3.53|P-value:2.12E-4|Clearance:0.02||SGD DESC:Type II HSP40 co-chaperone that interacts with the HSP70 protein Ssa1p; not functionally redundant with Ydj1p due to due to substrate specificity; has similarity to bacterial DnaJ proteins; protein abundance increases in response to DNA replication stress Gene:SPB4(YFL002C)|FD-Score:3.63|P-value:1.42E-4|Clearance:0.07||SGD DESC:Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients Gene:SPT14(YPL175W)|FD-Score:4.81|P-value:7.52E-7|Clearance:0.02||SGD DESC:UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins Gene:SRB4(YER022W)|FD-Score:-3.46|P-value:2.72E-4|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination Gene:SRB7(YDR308C)|FD-Score:-5.25|P-value:7.73E-8|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; target of the global repressor Tup1p Gene:SSY5(YJL156C)|FD-Score:11.7|P-value:9.35E-32|Clearance:0.02||SGD DESC:Serine protease of SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); contains an inhibitory domain that dissociates in response to extracellular amino acids, freeing a catalytic domain to activate transcription factor Stp1p Gene:SUI3(YPL237W)|FD-Score:9.29|P-value:7.47E-21|Clearance:0.23||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:TCP1(YDR212W)|FD-Score:-3.55|P-value:1.94E-4|Clearance:0||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:TFA1(YKL028W)|FD-Score:4.01|P-value:2.97E-5|Clearance:0||SGD DESC:TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening Gene:TIF35(YDR429C)|FD-Score:-3.74|P-value:9.09E-5|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TIM12(YBR091C)|FD-Score:7.91|P-value:1.31E-15|Clearance:0.03||SGD DESC:Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins Gene:TIM54(YJL054W)|FD-Score:10.5|P-value:4.13E-26|Clearance:0.88||SGD DESC:Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane Gene:TOM40(YMR203W)|FD-Score:-3.51|P-value:2.25E-4|Clearance:0||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:TOR2(YKL203C)|FD-Score:17.9|P-value:6.33E-72|Clearance:3.45||SGD DESC:PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication Gene:TRE2(YOR256C)|FD-Score:4.88|P-value:5.27E-7|Clearance:0.04||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication Gene:TRM5(YHR070W)|FD-Score:6.59|P-value:2.23E-11|Clearance:0.12||SGD DESC:tRNA(m(1)G37)methyltransferase, methylates a tRNA base adjacent to the anticodon that has a role in prevention of frameshifting; highly conserved across Archaea, Bacteria, and Eukarya Gene:TRS20(YBR254C)|FD-Score:4.11|P-value:2.00E-5|Clearance:0.06||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; mutations in the human homolog cause the spondyloepiphyseal dysplasia tarda (SEDL) disorder Gene:TRS31(YDR472W)|FD-Score:6.32|P-value:1.33E-10|Clearance:0.1||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:TUB1(YML085C)|FD-Score:4.19|P-value:1.38E-5|Clearance:0.05||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:TUB2(YFL037W)|FD-Score:-3.88|P-value:5.20E-5|Clearance:0||SGD DESC:Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria Gene:UTP15(YMR093W)|FD-Score:3.67|P-value:1.21E-4|Clearance:0.04||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:UTP18(YJL069C)|FD-Score:3.37|P-value:3.71E-4|Clearance:0.1||SGD DESC:Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data Gene:UTP22(YGR090W)|FD-Score:-4.66|P-value:1.57E-6|Clearance:0||SGD DESC:Component of the small-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals Gene:UTP9(YHR196W)|FD-Score:3.38|P-value:3.68E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YDL196W(YDL196W_d)|FD-Score:-5.22|P-value:8.98E-8|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YDR187C(YDR187C_d)|FD-Score:-3.47|P-value:2.59E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YDR526C(YDR526C_d)|FD-Score:-4.18|P-value:1.48E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR114C(YGR114C_d)|FD-Score:-4.55|P-value:2.69E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YGR115C(YGR115C_d)|FD-Score:6.47|P-value:5.02E-11|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YOR203W(YOR203W_d)|FD-Score:-3.55|P-value:1.94E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps 5' end of essential DED1 gene required for translation initiation Gene:YPL238C(YPL238C_d)|FD-Score:4.01|P-value:3.02E-5|Clearance:0.1||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Gene:YPT1(YFL038C)|FD-Score:6.68|P-value:1.19E-11|Clearance:0.08||SGD DESC:Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p) Gene:YTH1(YPR107C)|FD-Score:-5.15|P-value:1.31E-7|Clearance:0||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5284616
Download HOP data (tab-delimited text)  (excel)
Gene:AAT2(YLR027C)|FD-Score:12.2|P-value:2.52E-34||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:ACB1(YGR037C)|FD-Score:-3.74|P-value:9.20E-5||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ADE6(YGR061C)|FD-Score:-4.07|P-value:2.31E-5||SGD DESC:Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:AFT1(YGL071W)|FD-Score:-5.24|P-value:7.86E-8||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AFT2(YPL202C)|FD-Score:4.65|P-value:1.66E-6||SGD DESC:Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; AFT2 has a paralog, AFT1, that arose from the whole genome duplication Gene:AHP1(YLR109W)|FD-Score:-3.13|P-value:8.74E-4||SGD DESC:Thiol-specific peroxiredoxin, reduces hydroperoxides to protect against oxidative damage; function in vivo requires covalent conjugation to Urm1p Gene:AIM22(YJL046W)|FD-Score:3.18|P-value:7.30E-4||SGD DESC:Putative lipoate-protein ligase, required along with Lip2 and Lip5 for lipoylation of Lat1p and Kgd2p; similar to E. coli LplA; null mutant displays reduced frequency of mitochondrial genome loss Gene:ALD2(YMR170C)|FD-Score:3.31|P-value:4.64E-4||SGD DESC:Cytoplasmic aldehyde dehydrogenase, involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p Gene:APS1(YLR170C)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex Gene:ARC18(YLR370C)|FD-Score:5.78|P-value:3.70E-9||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARL3(YPL051W)|FD-Score:4.84|P-value:6.43E-7||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:ATP5(YDR298C)|FD-Score:-3.85|P-value:5.96E-5||SGD DESC:Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated Gene:ATS1(YAL020C)|FD-Score:9.71|P-value:1.38E-22||SGD DESC:Protein required, with Elongator complex, Kti11p, and Kti12p, for modification of wobble nucleosides in tRNA; has a potential role in regulatory interactions between microtubules and the cell cycle Gene:AVL9(YLR114C)|FD-Score:10.6|P-value:1.87E-26||SGD DESC:Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:BAR1(YIL015W)|FD-Score:5.85|P-value:2.47E-9||SGD DESC:Aspartyl protease secreted into the periplasmic space of mating type a cells, helps cells find mating partners, cleaves and inactivates alpha factor allowing cells to recover from alpha-factor-induced cell cycle arrest Gene:BCK1(YJL095W)|FD-Score:4.04|P-value:2.70E-5||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BDS1(YOL164W)|FD-Score:-3.68|P-value:1.16E-4||SGD DESC:Bacterially-derived sulfatase required for use of alkyl- and aryl-sulfates as sulfur sources Gene:BEM3(YPL115C)|FD-Score:4.01|P-value:3.00E-5||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly Gene:BEM4(YPL161C)|FD-Score:-5.4|P-value:3.29E-8||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BMH1(YER177W)|FD-Score:5.84|P-value:2.66E-9||SGD DESC:14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:BNI4(YNL233W)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p Gene:BRE5(YNR051C)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A Gene:BRO1(YPL084W)|FD-Score:6.08|P-value:6.07E-10||SGD DESC:Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes Gene:BRR1(YPR057W)|FD-Score:3.15|P-value:8.15E-4||SGD DESC:snRNP protein component of spliceosomal snRNPs, required for pre-mRNA splicing and snRNP biogenesis; in null mutant newly-synthesized snRNAs are destabilized and 3'-end processing is slowed Gene:BUD26(YDR241W_d)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 1% of ORF overlaps the verified gene SNU56; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:BUD27(YFL023W)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP Gene:BUD30(YDL151C_d)|FD-Score:4.09|P-value:2.14E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 96% of ORF overlaps the verified gene RPC53; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:BUL1(YMR275C)|FD-Score:3.21|P-value:6.71E-4||SGD DESC:Ubiquitin-binding component of the Rsp5p E3-ubiquitin ligase complex; disruption causes temperature-sensitive growth, overexpression causes missorting of amino acid permeases; BUL1 has a paralog, BUL2, that arose from the whole genome duplication Gene:CAF40(YNL288W)|FD-Score:7.37|P-value:8.83E-14||SGD DESC:Evolutionarily conserved subunit of the CCR4-NOT complex involved in controlling mRNA initiation, elongation and degradation; binds Cdc39p Gene:CCR4(YAL021C)|FD-Score:4.93|P-value:4.03E-7||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:CCS1(YMR038C)|FD-Score:-6.77|P-value:6.61E-12||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CIN8(YEL061C)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation Gene:CKA2(YOR061W)|FD-Score:5.54|P-value:1.54E-8||SGD DESC:Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching Gene:CLB5(YPR120C)|FD-Score:4.87|P-value:5.67E-7||SGD DESC:B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 phase Gene:CLC1(YGR167W)|FD-Score:8.12|P-value:2.38E-16||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:CLG1(YGL215W)|FD-Score:3.91|P-value:4.67E-5||SGD DESC:Cyclin-like protein that interacts with Pho85p; has sequence similarity to G1 cyclins PCL1 and PCL2 Gene:CNB1(YKL190W)|FD-Score:-8.35|P-value:3.53E-17||SGD DESC:Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress Gene:CNL1(YDR357C_p)|FD-Score:-3.69|P-value:1.12E-4||SGD DESC:Protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:CSF1(YLR087C)|FD-Score:-4.31|P-value:8.06E-6||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CTR1(YPR124W)|FD-Score:-4.85|P-value:6.05E-7||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CYS4(YGR155W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:DAL81(YIR023W)|FD-Score:3.79|P-value:7.43E-5||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DEG1(YFL001W)|FD-Score:5.39|P-value:3.56E-8||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability Gene:DEP1(YAL013W)|FD-Score:-3.34|P-value:4.15E-4||SGD DESC:Transcriptional modulator involved in regulation of structural phospholipid biosynthesis genes and metabolically unrelated genes, as well as maintenance of telomeres, mating efficiency, and sporulation Gene:DFG16(YOR030W)|FD-Score:-4.86|P-value:5.83E-7||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:DHH1(YDL160C)|FD-Score:10.1|P-value:4.51E-24||SGD DESC:Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, may have a role in mRNA export and translation; C-terminus of Dhh1p interacts with Ngr1p and promotes POR1, but not EDC1 mRNA decay; forms cytoplasmic foci upon DNA replication stress Gene:DID2(YKR035W-A)|FD-Score:7.39|P-value:7.33E-14||SGD DESC:Class E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors Gene:DID4(YKL002W)|FD-Score:5.05|P-value:2.26E-7||SGD DESC:Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis Gene:DIN7(YDR263C)|FD-Score:-3.93|P-value:4.33E-5||SGD DESC:Mitochondrial nuclease functioning in DNA repair and replication, modulates the stability of the mitochondrial genome, induced by exposure to mutagens, also induced during meiosis at a time nearly coincident with commitment to recombination Gene:DLS1(YJL065C)|FD-Score:3.75|P-value:8.67E-5||SGD DESC:Subunit of ISW2/yCHRAC chromatin accessibility complex; ISW2/yCHRAC also includes Itc1p, Isw2p, and Dpb4p; involved in inheritance of telomeric silencing; DLS1 has a paralog, DPB3, that arose from the whole genome duplication Gene:DOA1(YKL213C)|FD-Score:4.17|P-value:1.52E-5||SGD DESC:WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress Gene:DOA4(YDR069C)|FD-Score:6.98|P-value:1.53E-12||SGD DESC:Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole Gene:DPH1(YIL103W)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Protein required, along with Dph2p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph2p and Kti11p Gene:DXO1(YDR370C_p)|FD-Score:-3.24|P-value:6.00E-4||SGD DESC:Protein with decapping and 5′-3′ exoRNase activity; similar to Rai1p Gene:ECM33(YBR078W)|FD-Score:8.26|P-value:7.46E-17||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:EDE1(YBL047C)|FD-Score:5.66|P-value:7.63E-9||SGD DESC:Endocytic protein; involved in a network of interactions with other endocytic proteins, binds membranes in a ubiquitin-dependent manner, may also bind ubiquitinated membrane-associated proteins; interacts with Cmk2 and functions upstream of CMK2 in regulating non-apoptotic cell death Gene:ELP2(YGR200C)|FD-Score:10.4|P-value:1.70E-25||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin Gene:ELP3(YPL086C)|FD-Score:6.31|P-value:1.44E-10||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; exhibits histone acetyltransferase activity that is directed to histones H3 and H4; disruption confers resistance to K. lactis zymotoxin Gene:ELP4(YPL101W)|FD-Score:10.5|P-value:3.58E-26||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:ELP6(YMR312W)|FD-Score:7.89|P-value:1.56E-15||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:EMC2(YJR088C)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm Y57G7A.10/EMC-2, fly CG17556, human TTC35 Gene:END3(YNL084C)|FD-Score:14.5|P-value:1.09E-47||SGD DESC:EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p Gene:ERG2(YMR202W)|FD-Score:5.36|P-value:4.14E-8||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG4(YGL012W)|FD-Score:5.68|P-value:6.72E-9||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FAA2(YER015W)|FD-Score:-3.3|P-value:4.86E-4||SGD DESC:Medium chain fatty acyl-CoA synthetase, activates imported fatty acids; accepts a wide range of fatty acid chain lengths with a preference for medium chains, C9:0-C13:0; localized to the peroxisome Gene:FAB1(YFR019W)|FD-Score:5.46|P-value:2.42E-8||SGD DESC:1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis Gene:FKH2(YNL068C)|FD-Score:3.13|P-value:8.82E-4||SGD DESC:Forkhead family transcription factor; plays a major role in the expression of G2/M phase genes; positively regulates transcriptional elongation; negative role in chromatin silencing at HML and HMR; substrate of the Cdc28p/Clb5p kinase; FKH2 has a paralog, FKH1, that arose from the whole genome duplication Gene:FKS1(YLR342W)|FD-Score:4.58|P-value:2.32E-6||SGD DESC:Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling Gene:FPR1(YNL135C)|FD-Score:-16|P-value:6.23E-58||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase), binds to the drugs FK506 and rapamycin; also binds to the nonhistone chromatin binding protein Hmo1p and may regulate its assembly or function Gene:FPS1(YLL043W)|FD-Score:10.7|P-value:4.81E-27||SGD DESC:Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress Gene:GAL11(YOL051W)|FD-Score:-4.74|P-value:1.08E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GAT1(YFL021W)|FD-Score:-3.29|P-value:4.93E-4||SGD DESC:Transcriptional activator of genes involved in nitrogen catabolite repression; contains a GATA-1-type zinc finger DNA-binding motif; activity and localization regulated by nitrogen limitation and Ure2p Gene:GCN20(YFR009W)|FD-Score:-3.84|P-value:6.16E-5||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN5(YGR252W)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GCR2(YNL199C)|FD-Score:-6.11|P-value:4.87E-10||SGD DESC:Transcriptional activator of genes involved in glycolysis; interacts and functions with the DNA-binding protein Gcr1p Gene:GCS1(YDL226C)|FD-Score:-9.93|P-value:1.61E-23||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GDA1(YEL042W)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Guanosine diphosphatase located in the Golgi, involved in the transport of GDP-mannose into the Golgi lumen by converting GDP to GMP after mannose is transferred its substrate Gene:GGA2(YHR108W)|FD-Score:7.06|P-value:8.56E-13||SGD DESC:Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin Gene:GID8(YMR135C)|FD-Score:4.02|P-value:2.96E-5||SGD DESC:Subunit of GID Complex, binds strongly to central component Vid30p; GID Complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; recruits Rmd5p, Fyv10 and Vid28p to GID Complex; contains LisH, CTLH, and CRA domains that mediate binding to Vid30p (LisH) and Rmd5p and Vid28p (CTLH and CRA); dosage-dependent regulator of START Gene:GLE2(YER107C)|FD-Score:-3.18|P-value:7.34E-4||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GLN3(YER040W)|FD-Score:-5.46|P-value:2.34E-8||SGD DESC:Transcriptional activator of genes regulated by nitrogen catabolite repression (NCR), localization and activity regulated by quality of nitrogen source Gene:GOS1(YHL031C)|FD-Score:3.25|P-value:5.77E-4||SGD DESC:v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 Gene:GTF1(YGR102C)|FD-Score:-4.69|P-value:1.40E-6||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; transposon insertion mutant is salt sensitive and null mutant has growth defects; non-tagged protein is detected in purified mitochondria Gene:GTR1(YML121W)|FD-Score:16.6|P-value:4.26E-62||SGD DESC:Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB Gene:GTR2(YGR163W)|FD-Score:13|P-value:5.45E-39||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:GVP36(YIL041W)|FD-Score:3.84|P-value:6.24E-5||SGD DESC:BAR domain-containing protein that localizes to both early and late Golgi vesicles; required for adaptation to varying nutrient concentrations, fluid-phase endocytosis, polarization of the actin cytoskeleton, and vacuole biogenesis Gene:HFI1(YPL254W)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions Gene:HMG1(YML075C)|FD-Score:-4.47|P-value:3.85E-6||SGD DESC:HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress Gene:HOM2(YDR158W)|FD-Score:18.1|P-value:1.72E-73||SGD DESC:Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:HOM3(YER052C)|FD-Score:25.3|P-value:5.45E-141||SGD DESC:Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:HOM6(YJR139C)|FD-Score:12.5|P-value:6.93E-36||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:HSP78(YDR258C)|FD-Score:-3.56|P-value:1.88E-4||SGD DESC:Oligomeric mitochondrial matrix chaperone that cooperates with Ssc1p in mitochondrial thermotolerance after heat shock; able to prevent the aggregation of misfolded proteins as well as resolubilize protein aggregates Gene:IKI1(YHR187W)|FD-Score:15.7|P-value:8.27E-56||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin Gene:IKI3(YLR384C)|FD-Score:9.89|P-value:2.31E-23||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD) Gene:ILM1(YJR118C)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:IMP2'(YIL154C)|FD-Score:-3.53|P-value:2.08E-4||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:IPT1(YDR072C)|FD-Score:-3.34|P-value:4.23E-4||SGD DESC:Inositolphosphotransferase, involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin Gene:IRC13(YOR235W_d)|FD-Score:3.26|P-value:5.50E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci Gene:IRC21(YMR073C)|FD-Score:-8.14|P-value:2.04E-16||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:IRS4(YKR019C)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:IWR1(YDL115C)|FD-Score:3.26|P-value:5.55E-4||SGD DESC:RNA polymerase II transport factor, conserved from yeast to humans; also has a role in transporting RNA polymerase III into the nucleus; interacts with most of the RNAP II subunits; nucleo-cytoplasmic shuttling protein; deletion causes hypersensitivity to K1 killer toxin; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:JJJ3(YJR097W)|FD-Score:7.67|P-value:8.83E-15||SGD DESC:Protein of unknown function, contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4 Gene:KTI11(YBL071W-A)|FD-Score:4.03|P-value:2.78E-5||SGD DESC:Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs Gene:KTI12(YKL110C)|FD-Score:10.6|P-value:2.11E-26||SGD DESC:Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p Gene:LAP3(YNL239W)|FD-Score:4.66|P-value:1.61E-6||SGD DESC:Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH Gene:LDB19(YOR322C)|FD-Score:7.05|P-value:8.88E-13||SGD DESC:Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane; null mutant has reduced affinity for alcian blue dye Gene:LGE1(YPL055C)|FD-Score:-3.44|P-value:2.86E-4||SGD DESC:Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division Gene:LIP2(YLR239C)|FD-Score:3.13|P-value:8.73E-4||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:LSM1(YJL124C)|FD-Score:4.53|P-value:2.90E-6||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:LSM6(YDR378C)|FD-Score:4.11|P-value:1.95E-5||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:LSM7(YNL147W)|FD-Score:6.81|P-value:5.04E-12||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:MCT1(YOR221C)|FD-Score:4.42|P-value:4.93E-6||SGD DESC:Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling Gene:MDM34(YGL219C)|FD-Score:-3.82|P-value:6.59E-5||SGD DESC:Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MEH1(YKR007W)|FD-Score:10|P-value:7.71E-24||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; loss results in a defect in vacuolar acidification Gene:MEP1(YGR121C)|FD-Score:-3.4|P-value:3.43E-4||SGD DESC:Ammonium permease; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation Gene:MET22(YOL064C)|FD-Score:-3.96|P-value:3.74E-5||SGD DESC:Bisphosphate-3'-nucleotidase, involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway Gene:MFT1(YML062C)|FD-Score:5.48|P-value:2.15E-8||SGD DESC:Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance Gene:MID2(YLR332W)|FD-Score:3.86|P-value:5.74E-5||SGD DESC:O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p Gene:MLP1(YKR095W)|FD-Score:7.03|P-value:1.00E-12||SGD DESC:Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved with Tel1p in telomere length control; involved with Pml1p and Pml39p in nuclear retention of unspliced mRNAs Gene:MLP2(YIL149C)|FD-Score:3.72|P-value:9.78E-5||SGD DESC:Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length Gene:MOD5(YOR274W)|FD-Score:14|P-value:4.59E-45||SGD DESC:Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis Gene:MOT3(YMR070W)|FD-Score:6.23|P-value:2.27E-10||SGD DESC:Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion Gene:MRC1(YCL061C)|FD-Score:3.23|P-value:6.15E-4||SGD DESC:S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres Gene:MSN5(YDR335W)|FD-Score:-5.25|P-value:7.53E-8||SGD DESC:Karyopherin involved in nuclear import and export of proteins, including import of replication protein A and export of Swi6p, Far1p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm Gene:MSW1(YDR268W)|FD-Score:-6.43|P-value:6.17E-11||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:MTC2(YKL098W)|FD-Score:-3.3|P-value:4.89E-4||SGD DESC:Protein of unknown function; mtc2 is synthetically sick with cdc13-1 Gene:MTQ2(YDR140W)|FD-Score:-4.75|P-value:1.02E-6||SGD DESC:S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC Gene:NAT1(YDL040C)|FD-Score:6.07|P-value:6.46E-10||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:NBP2(YDR162C)|FD-Score:3.22|P-value:6.32E-4||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:NCL1(YBL024W)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120 Gene:NCS2(YNL119W)|FD-Score:12.3|P-value:3.44E-35||SGD DESC:Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:NCS6(YGL211W)|FD-Score:9.84|P-value:3.77E-23||SGD DESC:Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:NEM1(YHR004C)|FD-Score:4.04|P-value:2.62E-5||SGD DESC:Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard Gene:NPL3(YDR432W)|FD-Score:6.36|P-value:9.77E-11||SGD DESC:RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; has a role in repressing translation initiation by binding eIF4G; required for pre-mRNA splicing; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in the cytoplasm; protein abundance increases in response to DNA replication stress Gene:NPR1(YNL183C)|FD-Score:-4.14|P-value:1.75E-5||SGD DESC:Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex Gene:NRP1(YDL167C)|FD-Score:-4.53|P-value:2.89E-6||SGD DESC:Putative RNA binding protein of unknown function; localizes to stress granules induced by glucose deprivation; predicted to be involved in ribosome biogenesis Gene:OCA5(YHL029C_p)|FD-Score:5.9|P-value:1.78E-9||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:OPI8(YKR035C_d)|FD-Score:7.62|P-value:1.27E-14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A Gene:PAC10(YGR078C)|FD-Score:4.25|P-value:1.05E-5||SGD DESC:Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding Gene:PAR32(YDL173W)|FD-Score:5.72|P-value:5.32E-9||SGD DESC:Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene Gene:PCI8(YIL071C)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain Gene:PCL8(YPL219W)|FD-Score:3.25|P-value:5.79E-4||SGD DESC:Cyclin, interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation Gene:PDC1(YLR044C)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde; subject to glucose-, ethanol-, and autoregulation; involved in amino acid catabolism Gene:PEP7(YDR323C)|FD-Score:5.96|P-value:1.28E-9||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PEP8(YJL053W)|FD-Score:7.11|P-value:5.89E-13||SGD DESC:Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress Gene:PEX10(YDR265W)|FD-Score:-3.14|P-value:8.49E-4||SGD DESC:Peroxisomal membrane E3 ubiquitin ligase, required for for Ubc4p-dependent Pex5p ubiquitination and peroxisomal matrix protein import; contains zinc-binding RING domain; mutations in human homolog cause various peroxisomal disorders Gene:PFA5(YDR459C)|FD-Score:-3.24|P-value:6.02E-4||SGD DESC:Palmitoyltransferase with autoacylation activity; likely functions in pathway(s) outside Ras; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain Gene:PFK2(YMR205C)|FD-Score:-3.23|P-value:6.26E-4||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PGD1(YGL025C)|FD-Score:-10.2|P-value:8.31E-25||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PHO80(YOL001W)|FD-Score:6.14|P-value:4.12E-10||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling Gene:PHO81(YGR233C)|FD-Score:3.23|P-value:6.10E-4||SGD DESC:Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress Gene:PHO86(YJL117W)|FD-Score:-3.33|P-value:4.34E-4||SGD DESC:Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress Gene:PIB2(YGL023C)|FD-Score:3.16|P-value:7.76E-4||SGD DESC:Protein of unknown function; contains FYVE domain; similar to Fab1 and Vps27 Gene:PIN4(YBL051C)|FD-Score:7.33|P-value:1.16E-13||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:PKH2(YOL100W)|FD-Score:4.51|P-value:3.23E-6||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p Gene:PMR1(YGL167C)|FD-Score:-4.61|P-value:1.97E-6||SGD DESC:High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease Gene:PPM1(YDR435C)|FD-Score:-7.85|P-value:2.04E-15||SGD DESC:Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits Gene:PSK2(YOL045W)|FD-Score:-3.32|P-value:4.53E-4||SGD DESC:PAS-domain containing serine/threonine protein kinase; regulates sugar flux and translation in response to an unknown metabolite by phosphorylating Ugp1p and Gsy2p (sugar flux) and Caf20p, Tif11p and Sro9p (translation); PSK2 has a paralog, PSK1, that arose from the whole genome duplication Gene:PSY2(YNL201C)|FD-Score:-3.74|P-value:9.22E-5||SGD DESC:Subunit of protein phosphatase PP4 complex; active complex is composed of catalytic subunit Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3 Gene:PUF3(YLL013C)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins Gene:RAD33(YML011C)|FD-Score:4.44|P-value:4.47E-6||SGD DESC:Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:RAM1(YDL090C)|FD-Score:3.79|P-value:7.60E-5||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RAV1(YJR033C)|FD-Score:3.86|P-value:5.61E-5||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:RAV2(YDR202C)|FD-Score:3.9|P-value:4.88E-5||SGD DESC:Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme Gene:RCY1(YJL204C)|FD-Score:-6.24|P-value:2.16E-10||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RIM101(YHL027W)|FD-Score:-6.59|P-value:2.15E-11||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RIM13(YMR154C)|FD-Score:-4.23|P-value:1.14E-5||SGD DESC:Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB Gene:RIM15(YFL033C)|FD-Score:7.3|P-value:1.46E-13||SGD DESC:Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase Gene:RIM20(YOR275C)|FD-Score:-5.31|P-value:5.41E-8||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation Gene:RIM21(YNL294C)|FD-Score:-4.14|P-value:1.75E-5||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RIM8(YGL045W)|FD-Score:-4.84|P-value:6.49E-7||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:RIM9(YMR063W)|FD-Score:-7.3|P-value:1.39E-13||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RKM4(YDR257C)|FD-Score:-3.57|P-value:1.76E-4||SGD DESC:Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 55); nuclear SET-domain containing protein Gene:ROM2(YLR371W)|FD-Score:3.74|P-value:9.36E-5||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:ROX1(YPR065W)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Heme-dependent repressor of hypoxic genes; mediates aerobic transcriptional repression of hypoxia induced genes such as COX5b and CYC7; repressor function regulated through decreased promoter occupancy in response to oxidative stress; contains an HMG domain that is responsible for DNA bending activity; involved in the hyperosmotic stress resistance Gene:RPL12B(YDR418W)|FD-Score:3.25|P-value:5.79E-4||SGD DESC:Ribosomal 60S subunit protein L12B; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12B has a paralog, RPL12A, that arose from the whole genome duplication Gene:RPL14B(YHL001W)|FD-Score:-3.45|P-value:2.80E-4||SGD DESC:Ribosomal 60S subunit protein L14B; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14B has a paralog, RPL14A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPL20A(YMR242C)|FD-Score:-3.71|P-value:1.03E-4||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RPL34B(YIL052C)|FD-Score:8.13|P-value:2.18E-16||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:RPL35B(YDL136W)|FD-Score:-5.52|P-value:1.68E-8||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RPL6A(YML073C)|FD-Score:-3.6|P-value:1.61E-4||SGD DESC:Ribosomal 60S subunit protein L6A; N-terminally acetylated; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6A has a paralog, RPL6B, that arose from the whole genome duplication Gene:RPS24A(YER074W)|FD-Score:4.66|P-value:1.59E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication Gene:RPS25A(YGR027C)|FD-Score:4.66|P-value:1.61E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S25, no bacterial homolog; RPS25A has a paralog, RPS25B, that arose from the whole genome duplication Gene:RRD1(YIL153W)|FD-Score:-8.92|P-value:2.34E-19||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:RSA1(YPL193W)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly Gene:RSM18(YER050C)|FD-Score:-4.02|P-value:2.88E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein Gene:RTC3(YHR087W)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity; protein abundance increases in response to DNA replication stress Gene:RTG1(YOL067C)|FD-Score:4.54|P-value:2.82E-6||SGD DESC:Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus Gene:RTG2(YGL252C)|FD-Score:10.8|P-value:1.17E-27||SGD DESC:Sensor of mitochondrial dysfunction; regulates the subcellular location of Rtg1p and Rtg3p, transcriptional activators of the retrograde (RTG) and TOR pathways; Rtg2p is inhibited by the phosphorylated form of Mks1p Gene:RTG3(YBL103C)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Basic helix-loop-helix-leucine zipper (bHLH/Zip) transcription factor that forms a complex with another bHLH/Zip protein, Rtg1p, to activate the retrograde (RTG) and TOR pathways Gene:RTS3(YGR161C_p)|FD-Score:3.85|P-value:5.92E-5||SGD DESC:Putative component of the protein phosphatase type 2A complex Gene:RXT2(YBR095C)|FD-Score:-5.27|P-value:6.79E-8||SGD DESC:Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth Gene:RXT3(YDL076C)|FD-Score:-4.1|P-value:2.10E-5||SGD DESC:Subunit of the RPD3L complex; involved in histone deacetylation; protein abundance increases in response to DNA replication stress Gene:SAC1(YKL212W)|FD-Score:3.94|P-value:4.15E-5||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAC3(YDR159W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) Gene:SAC6(YDR129C)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SAM3(YPL274W)|FD-Score:3.18|P-value:7.40E-4||SGD DESC:High-affinity S-adenosylmethionine permease, required for utilization of S-adenosylmethionine as a sulfur source; has similarity to S-methylmethionine permease Mmp1p Gene:SBE2(YDR351W)|FD-Score:-3.5|P-value:2.29E-4||SGD DESC:Protein involved in the transport of cell wall components from the Golgi to the cell surface; required for bud growth Gene:SBP1(YHL034C)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Protein that binds eIF4G and has a role in repression of translation; has an RGG motif; found in cytoplasmic P bodies; found associated with small nucleolar RNAs snR10 and snR11 Gene:SDC1(YDR469W)|FD-Score:-4.88|P-value:5.30E-7||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30 Gene:SEC22(YLR268W)|FD-Score:-3.2|P-value:6.81E-4||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SEC66(YBR171W)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SED1(YDR077W)|FD-Score:-4.11|P-value:2.00E-5||SGD DESC:Major stress-induced structural GPI-cell wall glycoprotein in stationary-phase cells, associates with translating ribosomes, possible role in mitochondrial genome maintenance; ORF contains two distinct variable minisatellites Gene:SEE1(YIL064W)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Probable lysine methyltransferase involved in the dimethylation of eEF1A (Tef1p/Tef2p); sequence similarity to S-adenosylmethionine-dependent methyltransferases of the seven beta-strand family; role in vesicular transport Gene:SEM1(YDR363W-A)|FD-Score:4.61|P-value:1.97E-6||SGD DESC:Component of lid subcomplex of the 26S proteasome regulatory subunit; involved in mRNA export mediated by the TREX-2 complex (Sac3p-Thp1p); ortholog of human DSS1; protein abundance increases in response to DNA replication stress Gene:SER1(YOR184W)|FD-Score:9.49|P-value:1.15E-21||SGD DESC:3-phosphoserine aminotransferase; catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p; protein abundance increases in response to DNA replication stress Gene:SER2(YGR208W)|FD-Score:12.5|P-value:6.88E-36||SGD DESC:Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source Gene:SET2(YJL168C)|FD-Score:4.41|P-value:5.14E-6||SGD DESC:Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36, which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain of Rpo21p; histone methylation activity is regulated by phosphorylation status of Rpo21p Gene:SET3(YKR029C)|FD-Score:4.3|P-value:8.55E-6||SGD DESC:Defining member of the SET3 histone deacetylase complex which is a meiosis-specific repressor of sporulation genes; necessary for efficient transcription by RNAPII; one of two yeast proteins that contains both SET and PHD domains; SET3 has a paralog, SET4, that arose from the whole genome duplication Gene:SGT2(YOR007C)|FD-Score:-3.47|P-value:2.64E-4||SGD DESC:Glutamine-rich cytoplasmic cochaperone; serves as a scaffold bringing together Get4, Get5p, and other TRC complex members that are required to mediate posttranslational insertion of tail-anchored proteins into the ER membrane; interacts with the prion domain of Sup35p; amyloid sensor; plays a role in targeting chaperones to prion aggregates; has similarity to human cochaperone SGT; forms cytoplasmic foci upon DNA replication stress Gene:SHE4(YOR035C)|FD-Score:12.6|P-value:1.00E-36||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SHM2(YLR058C)|FD-Score:7.69|P-value:7.61E-15||SGD DESC:Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis Gene:SHU2(YDR078C)|FD-Score:-3.38|P-value:3.61E-4||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function Gene:SIN4(YNL236W)|FD-Score:-8.05|P-value:4.12E-16||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription Gene:SIP5(YMR140W)|FD-Score:4.38|P-value:5.80E-6||SGD DESC:Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase; forms cytoplasmic foci upon DNA replication stress Gene:SIZ1(YDR409W)|FD-Score:-3.52|P-value:2.12E-4||SGD DESC:SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring Gene:SKN7(YHR206W)|FD-Score:4.08|P-value:2.29E-5||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SLA1(YBL007C)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains Gene:SLG1(YOR008C)|FD-Score:9.26|P-value:1.02E-20||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SLM4(YBR077C)|FD-Score:17|P-value:4.42E-65||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4 Gene:SMI1(YGR229C)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:SNF11(YDR073W)|FD-Score:-3.82|P-value:6.55E-5||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; interacts with a highly conserved 40-residue sequence of Snf2p; relocates to the cytosol under hypoxic conditions Gene:SNF7(YLR025W)|FD-Score:7.33|P-value:1.13E-13||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SNQ2(YDR011W)|FD-Score:-3.27|P-value:5.44E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SNX3(YOR357C)|FD-Score:4.63|P-value:1.80E-6||SGD DESC:Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p Gene:SNX4(YJL036W)|FD-Score:4.56|P-value:2.52E-6||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SOD1(YJR104C)|FD-Score:-4.87|P-value:5.68E-7||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPF1(YEL031W)|FD-Score:-6.01|P-value:9.30E-10||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SPO7(YAL009W)|FD-Score:4.35|P-value:6.89E-6||SGD DESC:Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation Gene:SPT3(YDR392W)|FD-Score:-6.75|P-value:7.33E-12||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SPT8(YLR055C)|FD-Score:-6.24|P-value:2.16E-10||SGD DESC:Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters Gene:SRB5(YGR104C)|FD-Score:-4.4|P-value:5.45E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance Gene:SRN2(YLR119W)|FD-Score:7.01|P-value:1.20E-12||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus Gene:SSN2(YDR443C)|FD-Score:-3.5|P-value:2.35E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:SSQ1(YLR369W)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Mitochondrial hsp70-type molecular chaperone, required for assembly of iron/sulfur clusters into proteins at a step after cluster synthesis, and for maturation of Yfh1p, which is a homolog of human frataxin implicated in Friedreich's ataxia Gene:STP22(YCL008C)|FD-Score:5.27|P-value:6.91E-8||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:SUS1(YBR111W-A)|FD-Score:6.74|P-value:8.14E-12||SGD DESC:Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP Gene:SWF1(YDR126W)|FD-Score:7.06|P-value:8.11E-13||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:SWI3(YJL176C)|FD-Score:-5.65|P-value:8.16E-9||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; SWI/SNF regulates transcription by remodeling chromosomes; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; relocates to the cytosol under hypoxic conditions Gene:SYF2(YGR129W)|FD-Score:-4.19|P-value:1.42E-5||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:SYS1(YJL004C)|FD-Score:6.64|P-value:1.53E-11||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TAT1(YBR069C)|FD-Score:4.57|P-value:2.47E-6||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TAT2(YOL020W)|FD-Score:-3.38|P-value:3.68E-4||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:TCO89(YPL180W)|FD-Score:7.23|P-value:2.45E-13||SGD DESC:Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p), a complex that regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to rapamycin Gene:TEA1(YOR337W)|FD-Score:4.04|P-value:2.67E-5||SGD DESC:Ty1 enhancer activator required for full levels of Ty enhancer-mediated transcription; C6 zinc cluster DNA-binding protein Gene:THI74(YDR438W)|FD-Score:-3.13|P-value:8.66E-4||SGD DESC:Mitochondrial transporter repressible by thiamine; THI74 has a paralog, YML018C, that arose from the whole genome duplication Gene:THR1(YHR025W)|FD-Score:9.21|P-value:1.64E-20||SGD DESC:Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:THR4(YCR053W)|FD-Score:25.6|P-value:5.86E-145||SGD DESC:Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TIM18(YOR297C)|FD-Score:5.01|P-value:2.79E-7||SGD DESC:Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; may mediate assembly or stability of the complex Gene:TIP41(YPR040W)|FD-Score:-4.61|P-value:2.04E-6||SGD DESC:Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress Gene:TLG2(YOL018C)|FD-Score:5|P-value:2.88E-7||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TOR1(YJR066W)|FD-Score:12.9|P-value:3.11E-38||SGD DESC:PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication Gene:TPP1(YMR156C)|FD-Score:4.33|P-value:7.34E-6||SGD DESC:DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase Gene:TPS2(YDR074W)|FD-Score:-4.73|P-value:1.11E-6||SGD DESC:Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in synthesis of the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress Gene:TUM1(YOR251C)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Rhodanese domain sulfur transferase, accepts persulfite from Nfs1p and transfers it to Uba4p in the pathway for 2-thiolation of the wobble uridine base of tRNAs; also stimulates sulfur transfer by Nfs1p; may be mitochondrially localized Gene:TUS1(YLR425W)|FD-Score:3.74|P-value:9.09E-5||SGD DESC:Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate Gene:UBA4(YHR111W)|FD-Score:8.26|P-value:7.33E-17||SGD DESC:Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p Gene:UBI4(YLL039C)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Ubiquitin; becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-26S proteasome system; essential for the cellular stress response; encoded as a polyubiquitin precursor comprised of 5 head-to-tail repeats; protein abundance increases in response to DNA replication stress Gene:UBP3(YER151C)|FD-Score:3.92|P-value:4.37E-5||SGD DESC:Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress Gene:ULS1(YOR191W)|FD-Score:3.13|P-value:8.62E-4||SGD DESC:Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:UME1(YPL139C)|FD-Score:-3.29|P-value:4.99E-4||SGD DESC:Negative regulator of meiosis, required for repression of a subset of meiotic genes during vegetative growth, binding of histone deacetylase Rpd3p required for activity, contains a NEE box and a WD repeat motif; homologous with Wtm1p, Wtm2p Gene:UPC2(YDR213W)|FD-Score:-3.15|P-value:8.17E-4||SGD DESC:Sterol regulatory element binding protein; induces transcription of sterol biosynthetic genes and of DAN/TIR gene products; relocates from intracellular membranes to perinuclear foci on sterol depletion; UPC2 has a paralog, ECM22, that arose from the whole genome duplication Gene:URM1(YIL008W)|FD-Score:8.73|P-value:1.29E-18||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:VAC17(YCL063W)|FD-Score:4.4|P-value:5.41E-6||SGD DESC:Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p Gene:VAM10(YOR068C)|FD-Score:4.11|P-value:2.02E-5||SGD DESC:Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering Gene:VAM3(YOR106W)|FD-Score:4.13|P-value:1.81E-5||SGD DESC:Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region Gene:VAM6(YDL077C)|FD-Score:10.8|P-value:2.70E-27||SGD DESC:Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p Gene:VAM7(YGL212W)|FD-Score:12.7|P-value:2.27E-37||SGD DESC:Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress Gene:VID28(YIL017C)|FD-Score:3.73|P-value:9.47E-5||SGD DESC:GID Complex subunit, serves as adaptor for regulatory subunit Vid24p; protein involved in proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase); localized to the nucleus and the cytoplasm Gene:VMS1(YDR049W)|FD-Score:8.38|P-value:2.75E-17||SGD DESC:Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans Gene:VPS13(YLL040C)|FD-Score:4.82|P-value:7.19E-7||SGD DESC:Protein of unknown function; heterooligomeric or homooligomeric complex; peripherally associated with membranes; involved in sporulation, vacuolar protein sorting, prospore membrane formation and protein-Golgi retention; homologous to human CHAC and COH1 which are involved in chorea acanthocytosis and Cohen syndrome, respectively Gene:VPS17(YOR132W)|FD-Score:6.83|P-value:4.30E-12||SGD DESC:Subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde protein transport; peripheral membrane protein that assembles onto the membrane with Vps5p to promote vesicle formation; required for recruiting the retromer complex to the endosome membranes Gene:VPS24(YKL041W)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:VPS25(YJR102C)|FD-Score:5.66|P-value:7.60E-9||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS27(YNR006W)|FD-Score:6.98|P-value:1.45E-12||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:VPS29(YHR012W)|FD-Score:8.6|P-value:3.91E-18||SGD DESC:Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval Gene:VPS3(YDR495C)|FD-Score:6.3|P-value:1.45E-10||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS35(YJL154C)|FD-Score:12.1|P-value:3.45E-34||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:VPS4(YPR173C)|FD-Score:6.53|P-value:3.28E-11||SGD DESC:AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism Gene:VPS41(YDR080W)|FD-Score:8.13|P-value:2.07E-16||SGD DESC:Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport Gene:VPS5(YOR069W)|FD-Score:6.1|P-value:5.35E-10||SGD DESC:Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes Gene:VPS55(YJR044C)|FD-Score:3.18|P-value:7.29E-4||SGD DESC:Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP) Gene:VPS70(YJR126C)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:Protein of unknown function involved in vacuolar protein sorting Gene:VPS75(YNL246W)|FD-Score:5.08|P-value:1.84E-7||SGD DESC:NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress Gene:VPS9(YML097C)|FD-Score:4.77|P-value:9.32E-7||SGD DESC:A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin Gene:WHI3(YNL197C)|FD-Score:4.13|P-value:1.78E-5||SGD DESC:RNA binding protein that sequesters CLN3 mRNA in cytoplasmic foci;; Tpk1 (PKA) mediated phosphorylation of Whi3 affects G1/S transition by modulating Cln3 transcription; cytoplasmic retention factor for Cdc28p and associated cyclins; regulates cell fate and dose-dependently regulates the critical cell size required for passage through Start; WHI3 has a paralog, WHI4, that arose from the whole genome duplication Gene:YBL071C-B(YBL071C-B_p)|FD-Score:4.18|P-value:1.44E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBL071C(YBL071C_d)|FD-Score:5.41|P-value:3.24E-8||SGD DESC:Dubious open reading frame, predicted protein contains a peroxisomal targeting signal Gene:YBP2(YGL060W)|FD-Score:4|P-value:3.11E-5||SGD DESC:Central kinetochore associated protein; mediates mitotic progression; interacts with several central kinetochore proteins and centromeric histone Cse4p; role in resistance to oxidative stress; similar to Slk19p; YBP2 has a paralog, YBP1, that arose from the whole genome duplication Gene:YBR196C-A(YBR196C-A_p)|FD-Score:-4.33|P-value:7.34E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YCR075W-A(YCR075W-A_p)|FD-Score:6|P-value:9.95E-10||SGD DESC:Putative protein of unknown function; identified by homology to Ashbya gossypii; YCR075W-A has a paralog, YNR034W-A, that arose from the whole genome duplication Gene:YDL023C(YDL023C_d)|FD-Score:4.9|P-value:4.74E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in other Saccharomyces species; overlaps the verified gene GPD1; deletion confers sensitivity to GSAO; deletion in cyr1 mutant results in loss of stress resistance Gene:YDL041W(YDL041W_d)|FD-Score:6.83|P-value:4.32E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YDL172C(YDL172C_d)|FD-Score:9.6|P-value:4.12E-22||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL183C(YDL183C)|FD-Score:-4.96|P-value:3.53E-7||SGD DESC:Mitochondrial inner-membrane protein thought to be involved in the formation of an active mitochondrial K+/H+ exchanger (KHE) system; non-essential gene Gene:YDR290W(YDR290W_d)|FD-Score:-3.33|P-value:4.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Gene:YDR417C(YDR417C_d)|FD-Score:3.8|P-value:7.22E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RPL12B/YDR418W Gene:YEN1(YER041W)|FD-Score:-4.09|P-value:2.18E-5||SGD DESC:Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p Gene:YGL042C(YGL042C_d)|FD-Score:4.75|P-value:1.02E-6||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene DST1 Gene:YGL072C(YGL072C_d)|FD-Score:7.34|P-value:1.09E-13||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGL188C-A(YGL188C-A_p)|FD-Score:4.46|P-value:4.10E-6||SGD DESC:Putative protein of unknown function Gene:YGR111W(YGR111W_p)|FD-Score:-3.69|P-value:1.10E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YGR122W(YGR122W)|FD-Score:-4.63|P-value:1.87E-6||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YGR164W(YGR164W_d)|FD-Score:-5.19|P-value:1.04E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIA6(YIL006W)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Mitochondrial NAD+ transporter, involved in the transport of NAD+ into the mitochondria (see also YEA6); member of the mitochondrial carrier subfamily; disputed role as a pyruvate transporter; has putative mouse and human orthologs Gene:YJL193W(YJL193W_p)|FD-Score:-3.19|P-value:7.20E-4||SGD DESC:Putative protein of unknown function, predicted to encode a triose phosphate transporter subfamily member based on phylogenetic analysis; similar to YOR307C/SLY41; deletion mutant has a respiratory growth defect Gene:YKE2(YLR200W)|FD-Score:3.85|P-value:5.95E-5||SGD DESC:Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin Gene:YKL118W(YKL118W_d)|FD-Score:-3.36|P-value:3.84E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YKR018C(YKR018C_p)|FD-Score:-3.52|P-value:2.18E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication Gene:YLR149C(YLR149C_p)|FD-Score:-3.18|P-value:7.40E-4||SGD DESC:Protein of unknown function; overexpression causes a cell cycle delay or arrest; null mutation results in a decrease in plasma membrane electron transport; YLR149C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YLR171W(YLR171W_d)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR224W(YLR224W_p)|FD-Score:3.82|P-value:6.64E-5||SGD DESC:F-box protein and component of SCF ubiquitin ligase complexes involved in ubiquitin-dependent protein catabolism; readily monoubiquitinated in vitro by SCF-Ubc4 complexes; YLR224W is not an essential gene Gene:YLR434C(YLR434C_d)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W Gene:YML094C-A(YML094C-A_d)|FD-Score:4.67|P-value:1.49E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO Gene:YNL120C(YNL120C_d)|FD-Score:3.95|P-value:3.96E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion enhances replication of Brome mosaic virus in S. cerevisiae, but likely due to effects on the overlapping gene Gene:YNL235C(YNL235C_d)|FD-Score:-4.68|P-value:1.43E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex Gene:YNR005C(YNR005C_d)|FD-Score:5.97|P-value:1.19E-9||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL050C(YOL050C_d)|FD-Score:-5.95|P-value:1.36E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YOR1(YGR281W)|FD-Score:4.02|P-value:2.90E-5||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter mediates export of many different organic anions including oligomycin; similar to human cystic fibrosis transmembrane receptor (CFTR) Gene:YOR199W(YOR199W_d)|FD-Score:-3.53|P-value:2.11E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL062W(YPL062W_d)|FD-Score:-3.43|P-value:2.97E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation Gene:YPL102C(YPL102C_d)|FD-Score:8.4|P-value:2.29E-17||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4 Gene:YPR084W(YPR084W_p)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Putative protein of unknown function Gene:YPR123C(YPR123C_d)|FD-Score:-7.82|P-value:2.74E-15||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPR197C(YPR197C_d)|FD-Score:-4.71|P-value:1.23E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPT7(YML001W)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7 Gene:ZAP1(YJL056C)|FD-Score:-4.53|P-value:2.92E-6||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains Gene:ZUO1(YGR285C)|FD-Score:3.12|P-value:9.04E-4||SGD DESC:Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p Gene:AAT2(YLR027C)|FD-Score:12.2|P-value:2.52E-34||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:ACB1(YGR037C)|FD-Score:-3.74|P-value:9.20E-5||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ADE6(YGR061C)|FD-Score:-4.07|P-value:2.31E-5||SGD DESC:Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:AFT1(YGL071W)|FD-Score:-5.24|P-value:7.86E-8||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AFT2(YPL202C)|FD-Score:4.65|P-value:1.66E-6||SGD DESC:Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; AFT2 has a paralog, AFT1, that arose from the whole genome duplication Gene:AHP1(YLR109W)|FD-Score:-3.13|P-value:8.74E-4||SGD DESC:Thiol-specific peroxiredoxin, reduces hydroperoxides to protect against oxidative damage; function in vivo requires covalent conjugation to Urm1p Gene:AIM22(YJL046W)|FD-Score:3.18|P-value:7.30E-4||SGD DESC:Putative lipoate-protein ligase, required along with Lip2 and Lip5 for lipoylation of Lat1p and Kgd2p; similar to E. coli LplA; null mutant displays reduced frequency of mitochondrial genome loss Gene:ALD2(YMR170C)|FD-Score:3.31|P-value:4.64E-4||SGD DESC:Cytoplasmic aldehyde dehydrogenase, involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p Gene:APS1(YLR170C)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex Gene:ARC18(YLR370C)|FD-Score:5.78|P-value:3.70E-9||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARL3(YPL051W)|FD-Score:4.84|P-value:6.43E-7||SGD DESC:GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor Gene:ATP5(YDR298C)|FD-Score:-3.85|P-value:5.96E-5||SGD DESC:Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated Gene:ATS1(YAL020C)|FD-Score:9.71|P-value:1.38E-22||SGD DESC:Protein required, with Elongator complex, Kti11p, and Kti12p, for modification of wobble nucleosides in tRNA; has a potential role in regulatory interactions between microtubules and the cell cycle Gene:AVL9(YLR114C)|FD-Score:10.6|P-value:1.87E-26||SGD DESC:Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:BAR1(YIL015W)|FD-Score:5.85|P-value:2.47E-9||SGD DESC:Aspartyl protease secreted into the periplasmic space of mating type a cells, helps cells find mating partners, cleaves and inactivates alpha factor allowing cells to recover from alpha-factor-induced cell cycle arrest Gene:BCK1(YJL095W)|FD-Score:4.04|P-value:2.70E-5||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BDS1(YOL164W)|FD-Score:-3.68|P-value:1.16E-4||SGD DESC:Bacterially-derived sulfatase required for use of alkyl- and aryl-sulfates as sulfur sources Gene:BEM3(YPL115C)|FD-Score:4.01|P-value:3.00E-5||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly Gene:BEM4(YPL161C)|FD-Score:-5.4|P-value:3.29E-8||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BMH1(YER177W)|FD-Score:5.84|P-value:2.66E-9||SGD DESC:14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:BNI4(YNL233W)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p Gene:BRE5(YNR051C)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A Gene:BRO1(YPL084W)|FD-Score:6.08|P-value:6.07E-10||SGD DESC:Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes Gene:BRR1(YPR057W)|FD-Score:3.15|P-value:8.15E-4||SGD DESC:snRNP protein component of spliceosomal snRNPs, required for pre-mRNA splicing and snRNP biogenesis; in null mutant newly-synthesized snRNAs are destabilized and 3'-end processing is slowed Gene:BUD26(YDR241W_d)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 1% of ORF overlaps the verified gene SNU56; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:BUD27(YFL023W)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP Gene:BUD30(YDL151C_d)|FD-Score:4.09|P-value:2.14E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 96% of ORF overlaps the verified gene RPC53; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:BUL1(YMR275C)|FD-Score:3.21|P-value:6.71E-4||SGD DESC:Ubiquitin-binding component of the Rsp5p E3-ubiquitin ligase complex; disruption causes temperature-sensitive growth, overexpression causes missorting of amino acid permeases; BUL1 has a paralog, BUL2, that arose from the whole genome duplication Gene:CAF40(YNL288W)|FD-Score:7.37|P-value:8.83E-14||SGD DESC:Evolutionarily conserved subunit of the CCR4-NOT complex involved in controlling mRNA initiation, elongation and degradation; binds Cdc39p Gene:CCR4(YAL021C)|FD-Score:4.93|P-value:4.03E-7||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:CCS1(YMR038C)|FD-Score:-6.77|P-value:6.61E-12||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CIN8(YEL061C)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation Gene:CKA2(YOR061W)|FD-Score:5.54|P-value:1.54E-8||SGD DESC:Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching Gene:CLB5(YPR120C)|FD-Score:4.87|P-value:5.67E-7||SGD DESC:B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 phase Gene:CLC1(YGR167W)|FD-Score:8.12|P-value:2.38E-16||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:CLG1(YGL215W)|FD-Score:3.91|P-value:4.67E-5||SGD DESC:Cyclin-like protein that interacts with Pho85p; has sequence similarity to G1 cyclins PCL1 and PCL2 Gene:CNB1(YKL190W)|FD-Score:-8.35|P-value:3.53E-17||SGD DESC:Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress Gene:CNL1(YDR357C_p)|FD-Score:-3.69|P-value:1.12E-4||SGD DESC:Protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:CSF1(YLR087C)|FD-Score:-4.31|P-value:8.06E-6||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CTR1(YPR124W)|FD-Score:-4.85|P-value:6.05E-7||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CYS4(YGR155W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:DAL81(YIR023W)|FD-Score:3.79|P-value:7.43E-5||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DEG1(YFL001W)|FD-Score:5.39|P-value:3.56E-8||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability Gene:DEP1(YAL013W)|FD-Score:-3.34|P-value:4.15E-4||SGD DESC:Transcriptional modulator involved in regulation of structural phospholipid biosynthesis genes and metabolically unrelated genes, as well as maintenance of telomeres, mating efficiency, and sporulation Gene:DFG16(YOR030W)|FD-Score:-4.86|P-value:5.83E-7||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:DHH1(YDL160C)|FD-Score:10.1|P-value:4.51E-24||SGD DESC:Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, may have a role in mRNA export and translation; C-terminus of Dhh1p interacts with Ngr1p and promotes POR1, but not EDC1 mRNA decay; forms cytoplasmic foci upon DNA replication stress Gene:DID2(YKR035W-A)|FD-Score:7.39|P-value:7.33E-14||SGD DESC:Class E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors Gene:DID4(YKL002W)|FD-Score:5.05|P-value:2.26E-7||SGD DESC:Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis Gene:DIN7(YDR263C)|FD-Score:-3.93|P-value:4.33E-5||SGD DESC:Mitochondrial nuclease functioning in DNA repair and replication, modulates the stability of the mitochondrial genome, induced by exposure to mutagens, also induced during meiosis at a time nearly coincident with commitment to recombination Gene:DLS1(YJL065C)|FD-Score:3.75|P-value:8.67E-5||SGD DESC:Subunit of ISW2/yCHRAC chromatin accessibility complex; ISW2/yCHRAC also includes Itc1p, Isw2p, and Dpb4p; involved in inheritance of telomeric silencing; DLS1 has a paralog, DPB3, that arose from the whole genome duplication Gene:DOA1(YKL213C)|FD-Score:4.17|P-value:1.52E-5||SGD DESC:WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress Gene:DOA4(YDR069C)|FD-Score:6.98|P-value:1.53E-12||SGD DESC:Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole Gene:DPH1(YIL103W)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Protein required, along with Dph2p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph2p and Kti11p Gene:DXO1(YDR370C_p)|FD-Score:-3.24|P-value:6.00E-4||SGD DESC:Protein with decapping and 5′-3′ exoRNase activity; similar to Rai1p Gene:ECM33(YBR078W)|FD-Score:8.26|P-value:7.46E-17||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:EDE1(YBL047C)|FD-Score:5.66|P-value:7.63E-9||SGD DESC:Endocytic protein; involved in a network of interactions with other endocytic proteins, binds membranes in a ubiquitin-dependent manner, may also bind ubiquitinated membrane-associated proteins; interacts with Cmk2 and functions upstream of CMK2 in regulating non-apoptotic cell death Gene:ELP2(YGR200C)|FD-Score:10.4|P-value:1.70E-25||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin Gene:ELP3(YPL086C)|FD-Score:6.31|P-value:1.44E-10||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; exhibits histone acetyltransferase activity that is directed to histones H3 and H4; disruption confers resistance to K. lactis zymotoxin Gene:ELP4(YPL101W)|FD-Score:10.5|P-value:3.58E-26||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:ELP6(YMR312W)|FD-Score:7.89|P-value:1.56E-15||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:EMC2(YJR088C)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm Y57G7A.10/EMC-2, fly CG17556, human TTC35 Gene:END3(YNL084C)|FD-Score:14.5|P-value:1.09E-47||SGD DESC:EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p Gene:ERG2(YMR202W)|FD-Score:5.36|P-value:4.14E-8||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG4(YGL012W)|FD-Score:5.68|P-value:6.72E-9||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FAA2(YER015W)|FD-Score:-3.3|P-value:4.86E-4||SGD DESC:Medium chain fatty acyl-CoA synthetase, activates imported fatty acids; accepts a wide range of fatty acid chain lengths with a preference for medium chains, C9:0-C13:0; localized to the peroxisome Gene:FAB1(YFR019W)|FD-Score:5.46|P-value:2.42E-8||SGD DESC:1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis Gene:FKH2(YNL068C)|FD-Score:3.13|P-value:8.82E-4||SGD DESC:Forkhead family transcription factor; plays a major role in the expression of G2/M phase genes; positively regulates transcriptional elongation; negative role in chromatin silencing at HML and HMR; substrate of the Cdc28p/Clb5p kinase; FKH2 has a paralog, FKH1, that arose from the whole genome duplication Gene:FKS1(YLR342W)|FD-Score:4.58|P-value:2.32E-6||SGD DESC:Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling Gene:FPR1(YNL135C)|FD-Score:-16|P-value:6.23E-58||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase), binds to the drugs FK506 and rapamycin; also binds to the nonhistone chromatin binding protein Hmo1p and may regulate its assembly or function Gene:FPS1(YLL043W)|FD-Score:10.7|P-value:4.81E-27||SGD DESC:Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress Gene:GAL11(YOL051W)|FD-Score:-4.74|P-value:1.08E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GAT1(YFL021W)|FD-Score:-3.29|P-value:4.93E-4||SGD DESC:Transcriptional activator of genes involved in nitrogen catabolite repression; contains a GATA-1-type zinc finger DNA-binding motif; activity and localization regulated by nitrogen limitation and Ure2p Gene:GCN20(YFR009W)|FD-Score:-3.84|P-value:6.16E-5||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN5(YGR252W)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GCR2(YNL199C)|FD-Score:-6.11|P-value:4.87E-10||SGD DESC:Transcriptional activator of genes involved in glycolysis; interacts and functions with the DNA-binding protein Gcr1p Gene:GCS1(YDL226C)|FD-Score:-9.93|P-value:1.61E-23||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GDA1(YEL042W)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Guanosine diphosphatase located in the Golgi, involved in the transport of GDP-mannose into the Golgi lumen by converting GDP to GMP after mannose is transferred its substrate Gene:GGA2(YHR108W)|FD-Score:7.06|P-value:8.56E-13||SGD DESC:Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin Gene:GID8(YMR135C)|FD-Score:4.02|P-value:2.96E-5||SGD DESC:Subunit of GID Complex, binds strongly to central component Vid30p; GID Complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; recruits Rmd5p, Fyv10 and Vid28p to GID Complex; contains LisH, CTLH, and CRA domains that mediate binding to Vid30p (LisH) and Rmd5p and Vid28p (CTLH and CRA); dosage-dependent regulator of START Gene:GLE2(YER107C)|FD-Score:-3.18|P-value:7.34E-4||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GLN3(YER040W)|FD-Score:-5.46|P-value:2.34E-8||SGD DESC:Transcriptional activator of genes regulated by nitrogen catabolite repression (NCR), localization and activity regulated by quality of nitrogen source Gene:GOS1(YHL031C)|FD-Score:3.25|P-value:5.77E-4||SGD DESC:v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 Gene:GTF1(YGR102C)|FD-Score:-4.69|P-value:1.40E-6||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; transposon insertion mutant is salt sensitive and null mutant has growth defects; non-tagged protein is detected in purified mitochondria Gene:GTR1(YML121W)|FD-Score:16.6|P-value:4.26E-62||SGD DESC:Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB Gene:GTR2(YGR163W)|FD-Score:13|P-value:5.45E-39||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:GVP36(YIL041W)|FD-Score:3.84|P-value:6.24E-5||SGD DESC:BAR domain-containing protein that localizes to both early and late Golgi vesicles; required for adaptation to varying nutrient concentrations, fluid-phase endocytosis, polarization of the actin cytoskeleton, and vacuole biogenesis Gene:HFI1(YPL254W)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions Gene:HMG1(YML075C)|FD-Score:-4.47|P-value:3.85E-6||SGD DESC:HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress Gene:HOM2(YDR158W)|FD-Score:18.1|P-value:1.72E-73||SGD DESC:Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:HOM3(YER052C)|FD-Score:25.3|P-value:5.45E-141||SGD DESC:Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:HOM6(YJR139C)|FD-Score:12.5|P-value:6.93E-36||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:HSP78(YDR258C)|FD-Score:-3.56|P-value:1.88E-4||SGD DESC:Oligomeric mitochondrial matrix chaperone that cooperates with Ssc1p in mitochondrial thermotolerance after heat shock; able to prevent the aggregation of misfolded proteins as well as resolubilize protein aggregates Gene:IKI1(YHR187W)|FD-Score:15.7|P-value:8.27E-56||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin Gene:IKI3(YLR384C)|FD-Score:9.89|P-value:2.31E-23||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD) Gene:ILM1(YJR118C)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:IMP2'(YIL154C)|FD-Score:-3.53|P-value:2.08E-4||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:IPT1(YDR072C)|FD-Score:-3.34|P-value:4.23E-4||SGD DESC:Inositolphosphotransferase, involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin Gene:IRC13(YOR235W_d)|FD-Score:3.26|P-value:5.50E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci Gene:IRC21(YMR073C)|FD-Score:-8.14|P-value:2.04E-16||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:IRS4(YKR019C)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:IWR1(YDL115C)|FD-Score:3.26|P-value:5.55E-4||SGD DESC:RNA polymerase II transport factor, conserved from yeast to humans; also has a role in transporting RNA polymerase III into the nucleus; interacts with most of the RNAP II subunits; nucleo-cytoplasmic shuttling protein; deletion causes hypersensitivity to K1 killer toxin; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:JJJ3(YJR097W)|FD-Score:7.67|P-value:8.83E-15||SGD DESC:Protein of unknown function, contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4 Gene:KTI11(YBL071W-A)|FD-Score:4.03|P-value:2.78E-5||SGD DESC:Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs Gene:KTI12(YKL110C)|FD-Score:10.6|P-value:2.11E-26||SGD DESC:Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p Gene:LAP3(YNL239W)|FD-Score:4.66|P-value:1.61E-6||SGD DESC:Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH Gene:LDB19(YOR322C)|FD-Score:7.05|P-value:8.88E-13||SGD DESC:Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane; null mutant has reduced affinity for alcian blue dye Gene:LGE1(YPL055C)|FD-Score:-3.44|P-value:2.86E-4||SGD DESC:Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division Gene:LIP2(YLR239C)|FD-Score:3.13|P-value:8.73E-4||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:LSM1(YJL124C)|FD-Score:4.53|P-value:2.90E-6||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:LSM6(YDR378C)|FD-Score:4.11|P-value:1.95E-5||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:LSM7(YNL147W)|FD-Score:6.81|P-value:5.04E-12||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:MCT1(YOR221C)|FD-Score:4.42|P-value:4.93E-6||SGD DESC:Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling Gene:MDM34(YGL219C)|FD-Score:-3.82|P-value:6.59E-5||SGD DESC:Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MEH1(YKR007W)|FD-Score:10|P-value:7.71E-24||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; loss results in a defect in vacuolar acidification Gene:MEP1(YGR121C)|FD-Score:-3.4|P-value:3.43E-4||SGD DESC:Ammonium permease; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation Gene:MET22(YOL064C)|FD-Score:-3.96|P-value:3.74E-5||SGD DESC:Bisphosphate-3'-nucleotidase, involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway Gene:MFT1(YML062C)|FD-Score:5.48|P-value:2.15E-8||SGD DESC:Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance Gene:MID2(YLR332W)|FD-Score:3.86|P-value:5.74E-5||SGD DESC:O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p Gene:MLP1(YKR095W)|FD-Score:7.03|P-value:1.00E-12||SGD DESC:Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved with Tel1p in telomere length control; involved with Pml1p and Pml39p in nuclear retention of unspliced mRNAs Gene:MLP2(YIL149C)|FD-Score:3.72|P-value:9.78E-5||SGD DESC:Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length Gene:MOD5(YOR274W)|FD-Score:14|P-value:4.59E-45||SGD DESC:Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis Gene:MOT3(YMR070W)|FD-Score:6.23|P-value:2.27E-10||SGD DESC:Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion Gene:MRC1(YCL061C)|FD-Score:3.23|P-value:6.15E-4||SGD DESC:S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres Gene:MSN5(YDR335W)|FD-Score:-5.25|P-value:7.53E-8||SGD DESC:Karyopherin involved in nuclear import and export of proteins, including import of replication protein A and export of Swi6p, Far1p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm Gene:MSW1(YDR268W)|FD-Score:-6.43|P-value:6.17E-11||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:MTC2(YKL098W)|FD-Score:-3.3|P-value:4.89E-4||SGD DESC:Protein of unknown function; mtc2 is synthetically sick with cdc13-1 Gene:MTQ2(YDR140W)|FD-Score:-4.75|P-value:1.02E-6||SGD DESC:S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC Gene:NAT1(YDL040C)|FD-Score:6.07|P-value:6.46E-10||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:NBP2(YDR162C)|FD-Score:3.22|P-value:6.32E-4||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:NCL1(YBL024W)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120 Gene:NCS2(YNL119W)|FD-Score:12.3|P-value:3.44E-35||SGD DESC:Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:NCS6(YGL211W)|FD-Score:9.84|P-value:3.77E-23||SGD DESC:Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:NEM1(YHR004C)|FD-Score:4.04|P-value:2.62E-5||SGD DESC:Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard Gene:NPL3(YDR432W)|FD-Score:6.36|P-value:9.77E-11||SGD DESC:RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; has a role in repressing translation initiation by binding eIF4G; required for pre-mRNA splicing; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in the cytoplasm; protein abundance increases in response to DNA replication stress Gene:NPR1(YNL183C)|FD-Score:-4.14|P-value:1.75E-5||SGD DESC:Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex Gene:NRP1(YDL167C)|FD-Score:-4.53|P-value:2.89E-6||SGD DESC:Putative RNA binding protein of unknown function; localizes to stress granules induced by glucose deprivation; predicted to be involved in ribosome biogenesis Gene:OCA5(YHL029C_p)|FD-Score:5.9|P-value:1.78E-9||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:OPI8(YKR035C_d)|FD-Score:7.62|P-value:1.27E-14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A Gene:PAC10(YGR078C)|FD-Score:4.25|P-value:1.05E-5||SGD DESC:Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding Gene:PAR32(YDL173W)|FD-Score:5.72|P-value:5.32E-9||SGD DESC:Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene Gene:PCI8(YIL071C)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain Gene:PCL8(YPL219W)|FD-Score:3.25|P-value:5.79E-4||SGD DESC:Cyclin, interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation Gene:PDC1(YLR044C)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde; subject to glucose-, ethanol-, and autoregulation; involved in amino acid catabolism Gene:PEP7(YDR323C)|FD-Score:5.96|P-value:1.28E-9||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PEP8(YJL053W)|FD-Score:7.11|P-value:5.89E-13||SGD DESC:Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress Gene:PEX10(YDR265W)|FD-Score:-3.14|P-value:8.49E-4||SGD DESC:Peroxisomal membrane E3 ubiquitin ligase, required for for Ubc4p-dependent Pex5p ubiquitination and peroxisomal matrix protein import; contains zinc-binding RING domain; mutations in human homolog cause various peroxisomal disorders Gene:PFA5(YDR459C)|FD-Score:-3.24|P-value:6.02E-4||SGD DESC:Palmitoyltransferase with autoacylation activity; likely functions in pathway(s) outside Ras; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain Gene:PFK2(YMR205C)|FD-Score:-3.23|P-value:6.26E-4||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PGD1(YGL025C)|FD-Score:-10.2|P-value:8.31E-25||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PHO80(YOL001W)|FD-Score:6.14|P-value:4.12E-10||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling Gene:PHO81(YGR233C)|FD-Score:3.23|P-value:6.10E-4||SGD DESC:Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress Gene:PHO86(YJL117W)|FD-Score:-3.33|P-value:4.34E-4||SGD DESC:Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress Gene:PIB2(YGL023C)|FD-Score:3.16|P-value:7.76E-4||SGD DESC:Protein of unknown function; contains FYVE domain; similar to Fab1 and Vps27 Gene:PIN4(YBL051C)|FD-Score:7.33|P-value:1.16E-13||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:PKH2(YOL100W)|FD-Score:4.51|P-value:3.23E-6||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p Gene:PMR1(YGL167C)|FD-Score:-4.61|P-value:1.97E-6||SGD DESC:High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease Gene:PPM1(YDR435C)|FD-Score:-7.85|P-value:2.04E-15||SGD DESC:Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits Gene:PSK2(YOL045W)|FD-Score:-3.32|P-value:4.53E-4||SGD DESC:PAS-domain containing serine/threonine protein kinase; regulates sugar flux and translation in response to an unknown metabolite by phosphorylating Ugp1p and Gsy2p (sugar flux) and Caf20p, Tif11p and Sro9p (translation); PSK2 has a paralog, PSK1, that arose from the whole genome duplication Gene:PSY2(YNL201C)|FD-Score:-3.74|P-value:9.22E-5||SGD DESC:Subunit of protein phosphatase PP4 complex; active complex is composed of catalytic subunit Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3 Gene:PUF3(YLL013C)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins Gene:RAD33(YML011C)|FD-Score:4.44|P-value:4.47E-6||SGD DESC:Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:RAM1(YDL090C)|FD-Score:3.79|P-value:7.60E-5||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RAV1(YJR033C)|FD-Score:3.86|P-value:5.61E-5||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:RAV2(YDR202C)|FD-Score:3.9|P-value:4.88E-5||SGD DESC:Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme Gene:RCY1(YJL204C)|FD-Score:-6.24|P-value:2.16E-10||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RIM101(YHL027W)|FD-Score:-6.59|P-value:2.15E-11||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RIM13(YMR154C)|FD-Score:-4.23|P-value:1.14E-5||SGD DESC:Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB Gene:RIM15(YFL033C)|FD-Score:7.3|P-value:1.46E-13||SGD DESC:Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase Gene:RIM20(YOR275C)|FD-Score:-5.31|P-value:5.41E-8||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation Gene:RIM21(YNL294C)|FD-Score:-4.14|P-value:1.75E-5||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RIM8(YGL045W)|FD-Score:-4.84|P-value:6.49E-7||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:RIM9(YMR063W)|FD-Score:-7.3|P-value:1.39E-13||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RKM4(YDR257C)|FD-Score:-3.57|P-value:1.76E-4||SGD DESC:Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 55); nuclear SET-domain containing protein Gene:ROM2(YLR371W)|FD-Score:3.74|P-value:9.36E-5||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:ROX1(YPR065W)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Heme-dependent repressor of hypoxic genes; mediates aerobic transcriptional repression of hypoxia induced genes such as COX5b and CYC7; repressor function regulated through decreased promoter occupancy in response to oxidative stress; contains an HMG domain that is responsible for DNA bending activity; involved in the hyperosmotic stress resistance Gene:RPL12B(YDR418W)|FD-Score:3.25|P-value:5.79E-4||SGD DESC:Ribosomal 60S subunit protein L12B; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12B has a paralog, RPL12A, that arose from the whole genome duplication Gene:RPL14B(YHL001W)|FD-Score:-3.45|P-value:2.80E-4||SGD DESC:Ribosomal 60S subunit protein L14B; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14B has a paralog, RPL14A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPL20A(YMR242C)|FD-Score:-3.71|P-value:1.03E-4||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RPL34B(YIL052C)|FD-Score:8.13|P-value:2.18E-16||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:RPL35B(YDL136W)|FD-Score:-5.52|P-value:1.68E-8||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RPL6A(YML073C)|FD-Score:-3.6|P-value:1.61E-4||SGD DESC:Ribosomal 60S subunit protein L6A; N-terminally acetylated; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6A has a paralog, RPL6B, that arose from the whole genome duplication Gene:RPS24A(YER074W)|FD-Score:4.66|P-value:1.59E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication Gene:RPS25A(YGR027C)|FD-Score:4.66|P-value:1.61E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S25, no bacterial homolog; RPS25A has a paralog, RPS25B, that arose from the whole genome duplication Gene:RRD1(YIL153W)|FD-Score:-8.92|P-value:2.34E-19||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:RSA1(YPL193W)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly Gene:RSM18(YER050C)|FD-Score:-4.02|P-value:2.88E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein Gene:RTC3(YHR087W)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity; protein abundance increases in response to DNA replication stress Gene:RTG1(YOL067C)|FD-Score:4.54|P-value:2.82E-6||SGD DESC:Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus Gene:RTG2(YGL252C)|FD-Score:10.8|P-value:1.17E-27||SGD DESC:Sensor of mitochondrial dysfunction; regulates the subcellular location of Rtg1p and Rtg3p, transcriptional activators of the retrograde (RTG) and TOR pathways; Rtg2p is inhibited by the phosphorylated form of Mks1p Gene:RTG3(YBL103C)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Basic helix-loop-helix-leucine zipper (bHLH/Zip) transcription factor that forms a complex with another bHLH/Zip protein, Rtg1p, to activate the retrograde (RTG) and TOR pathways Gene:RTS3(YGR161C_p)|FD-Score:3.85|P-value:5.92E-5||SGD DESC:Putative component of the protein phosphatase type 2A complex Gene:RXT2(YBR095C)|FD-Score:-5.27|P-value:6.79E-8||SGD DESC:Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth Gene:RXT3(YDL076C)|FD-Score:-4.1|P-value:2.10E-5||SGD DESC:Subunit of the RPD3L complex; involved in histone deacetylation; protein abundance increases in response to DNA replication stress Gene:SAC1(YKL212W)|FD-Score:3.94|P-value:4.15E-5||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAC3(YDR159W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) Gene:SAC6(YDR129C)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SAM3(YPL274W)|FD-Score:3.18|P-value:7.40E-4||SGD DESC:High-affinity S-adenosylmethionine permease, required for utilization of S-adenosylmethionine as a sulfur source; has similarity to S-methylmethionine permease Mmp1p Gene:SBE2(YDR351W)|FD-Score:-3.5|P-value:2.29E-4||SGD DESC:Protein involved in the transport of cell wall components from the Golgi to the cell surface; required for bud growth Gene:SBP1(YHL034C)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Protein that binds eIF4G and has a role in repression of translation; has an RGG motif; found in cytoplasmic P bodies; found associated with small nucleolar RNAs snR10 and snR11 Gene:SDC1(YDR469W)|FD-Score:-4.88|P-value:5.30E-7||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30 Gene:SEC22(YLR268W)|FD-Score:-3.2|P-value:6.81E-4||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SEC66(YBR171W)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SED1(YDR077W)|FD-Score:-4.11|P-value:2.00E-5||SGD DESC:Major stress-induced structural GPI-cell wall glycoprotein in stationary-phase cells, associates with translating ribosomes, possible role in mitochondrial genome maintenance; ORF contains two distinct variable minisatellites Gene:SEE1(YIL064W)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Probable lysine methyltransferase involved in the dimethylation of eEF1A (Tef1p/Tef2p); sequence similarity to S-adenosylmethionine-dependent methyltransferases of the seven beta-strand family; role in vesicular transport Gene:SEM1(YDR363W-A)|FD-Score:4.61|P-value:1.97E-6||SGD DESC:Component of lid subcomplex of the 26S proteasome regulatory subunit; involved in mRNA export mediated by the TREX-2 complex (Sac3p-Thp1p); ortholog of human DSS1; protein abundance increases in response to DNA replication stress Gene:SER1(YOR184W)|FD-Score:9.49|P-value:1.15E-21||SGD DESC:3-phosphoserine aminotransferase; catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p; protein abundance increases in response to DNA replication stress Gene:SER2(YGR208W)|FD-Score:12.5|P-value:6.88E-36||SGD DESC:Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source Gene:SET2(YJL168C)|FD-Score:4.41|P-value:5.14E-6||SGD DESC:Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36, which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain of Rpo21p; histone methylation activity is regulated by phosphorylation status of Rpo21p Gene:SET3(YKR029C)|FD-Score:4.3|P-value:8.55E-6||SGD DESC:Defining member of the SET3 histone deacetylase complex which is a meiosis-specific repressor of sporulation genes; necessary for efficient transcription by RNAPII; one of two yeast proteins that contains both SET and PHD domains; SET3 has a paralog, SET4, that arose from the whole genome duplication Gene:SGT2(YOR007C)|FD-Score:-3.47|P-value:2.64E-4||SGD DESC:Glutamine-rich cytoplasmic cochaperone; serves as a scaffold bringing together Get4, Get5p, and other TRC complex members that are required to mediate posttranslational insertion of tail-anchored proteins into the ER membrane; interacts with the prion domain of Sup35p; amyloid sensor; plays a role in targeting chaperones to prion aggregates; has similarity to human cochaperone SGT; forms cytoplasmic foci upon DNA replication stress Gene:SHE4(YOR035C)|FD-Score:12.6|P-value:1.00E-36||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SHM2(YLR058C)|FD-Score:7.69|P-value:7.61E-15||SGD DESC:Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis Gene:SHU2(YDR078C)|FD-Score:-3.38|P-value:3.61E-4||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function Gene:SIN4(YNL236W)|FD-Score:-8.05|P-value:4.12E-16||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription Gene:SIP5(YMR140W)|FD-Score:4.38|P-value:5.80E-6||SGD DESC:Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase; forms cytoplasmic foci upon DNA replication stress Gene:SIZ1(YDR409W)|FD-Score:-3.52|P-value:2.12E-4||SGD DESC:SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring Gene:SKN7(YHR206W)|FD-Score:4.08|P-value:2.29E-5||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SLA1(YBL007C)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains Gene:SLG1(YOR008C)|FD-Score:9.26|P-value:1.02E-20||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SLM4(YBR077C)|FD-Score:17|P-value:4.42E-65||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4 Gene:SMI1(YGR229C)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:SNF11(YDR073W)|FD-Score:-3.82|P-value:6.55E-5||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; interacts with a highly conserved 40-residue sequence of Snf2p; relocates to the cytosol under hypoxic conditions Gene:SNF7(YLR025W)|FD-Score:7.33|P-value:1.13E-13||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SNQ2(YDR011W)|FD-Score:-3.27|P-value:5.44E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SNX3(YOR357C)|FD-Score:4.63|P-value:1.80E-6||SGD DESC:Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p Gene:SNX4(YJL036W)|FD-Score:4.56|P-value:2.52E-6||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SOD1(YJR104C)|FD-Score:-4.87|P-value:5.68E-7||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPF1(YEL031W)|FD-Score:-6.01|P-value:9.30E-10||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SPO7(YAL009W)|FD-Score:4.35|P-value:6.89E-6||SGD DESC:Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation Gene:SPT3(YDR392W)|FD-Score:-6.75|P-value:7.33E-12||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SPT8(YLR055C)|FD-Score:-6.24|P-value:2.16E-10||SGD DESC:Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters Gene:SRB5(YGR104C)|FD-Score:-4.4|P-value:5.45E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance Gene:SRN2(YLR119W)|FD-Score:7.01|P-value:1.20E-12||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus Gene:SSN2(YDR443C)|FD-Score:-3.5|P-value:2.35E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:SSQ1(YLR369W)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Mitochondrial hsp70-type molecular chaperone, required for assembly of iron/sulfur clusters into proteins at a step after cluster synthesis, and for maturation of Yfh1p, which is a homolog of human frataxin implicated in Friedreich's ataxia Gene:STP22(YCL008C)|FD-Score:5.27|P-value:6.91E-8||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:SUS1(YBR111W-A)|FD-Score:6.74|P-value:8.14E-12||SGD DESC:Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP Gene:SWF1(YDR126W)|FD-Score:7.06|P-value:8.11E-13||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:SWI3(YJL176C)|FD-Score:-5.65|P-value:8.16E-9||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; SWI/SNF regulates transcription by remodeling chromosomes; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; relocates to the cytosol under hypoxic conditions Gene:SYF2(YGR129W)|FD-Score:-4.19|P-value:1.42E-5||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:SYS1(YJL004C)|FD-Score:6.64|P-value:1.53E-11||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TAT1(YBR069C)|FD-Score:4.57|P-value:2.47E-6||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TAT2(YOL020W)|FD-Score:-3.38|P-value:3.68E-4||SGD DESC:High affinity tryptophan and tyrosine permease, overexpression confers FK506 and FTY720 resistance Gene:TCO89(YPL180W)|FD-Score:7.23|P-value:2.45E-13||SGD DESC:Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p), a complex that regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to rapamycin Gene:TEA1(YOR337W)|FD-Score:4.04|P-value:2.67E-5||SGD DESC:Ty1 enhancer activator required for full levels of Ty enhancer-mediated transcription; C6 zinc cluster DNA-binding protein Gene:THI74(YDR438W)|FD-Score:-3.13|P-value:8.66E-4||SGD DESC:Mitochondrial transporter repressible by thiamine; THI74 has a paralog, YML018C, that arose from the whole genome duplication Gene:THR1(YHR025W)|FD-Score:9.21|P-value:1.64E-20||SGD DESC:Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:THR4(YCR053W)|FD-Score:25.6|P-value:5.86E-145||SGD DESC:Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TIM18(YOR297C)|FD-Score:5.01|P-value:2.79E-7||SGD DESC:Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; may mediate assembly or stability of the complex Gene:TIP41(YPR040W)|FD-Score:-4.61|P-value:2.04E-6||SGD DESC:Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress Gene:TLG2(YOL018C)|FD-Score:5|P-value:2.88E-7||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TOR1(YJR066W)|FD-Score:12.9|P-value:3.11E-38||SGD DESC:PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication Gene:TPP1(YMR156C)|FD-Score:4.33|P-value:7.34E-6||SGD DESC:DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase Gene:TPS2(YDR074W)|FD-Score:-4.73|P-value:1.11E-6||SGD DESC:Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in synthesis of the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress Gene:TUM1(YOR251C)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Rhodanese domain sulfur transferase, accepts persulfite from Nfs1p and transfers it to Uba4p in the pathway for 2-thiolation of the wobble uridine base of tRNAs; also stimulates sulfur transfer by Nfs1p; may be mitochondrially localized Gene:TUS1(YLR425W)|FD-Score:3.74|P-value:9.09E-5||SGD DESC:Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate Gene:UBA4(YHR111W)|FD-Score:8.26|P-value:7.33E-17||SGD DESC:Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p Gene:UBI4(YLL039C)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Ubiquitin; becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-26S proteasome system; essential for the cellular stress response; encoded as a polyubiquitin precursor comprised of 5 head-to-tail repeats; protein abundance increases in response to DNA replication stress Gene:UBP3(YER151C)|FD-Score:3.92|P-value:4.37E-5||SGD DESC:Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress Gene:ULS1(YOR191W)|FD-Score:3.13|P-value:8.62E-4||SGD DESC:Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:UME1(YPL139C)|FD-Score:-3.29|P-value:4.99E-4||SGD DESC:Negative regulator of meiosis, required for repression of a subset of meiotic genes during vegetative growth, binding of histone deacetylase Rpd3p required for activity, contains a NEE box and a WD repeat motif; homologous with Wtm1p, Wtm2p Gene:UPC2(YDR213W)|FD-Score:-3.15|P-value:8.17E-4||SGD DESC:Sterol regulatory element binding protein; induces transcription of sterol biosynthetic genes and of DAN/TIR gene products; relocates from intracellular membranes to perinuclear foci on sterol depletion; UPC2 has a paralog, ECM22, that arose from the whole genome duplication Gene:URM1(YIL008W)|FD-Score:8.73|P-value:1.29E-18||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:VAC17(YCL063W)|FD-Score:4.4|P-value:5.41E-6||SGD DESC:Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p Gene:VAM10(YOR068C)|FD-Score:4.11|P-value:2.02E-5||SGD DESC:Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering Gene:VAM3(YOR106W)|FD-Score:4.13|P-value:1.81E-5||SGD DESC:Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region Gene:VAM6(YDL077C)|FD-Score:10.8|P-value:2.70E-27||SGD DESC:Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p Gene:VAM7(YGL212W)|FD-Score:12.7|P-value:2.27E-37||SGD DESC:Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress Gene:VID28(YIL017C)|FD-Score:3.73|P-value:9.47E-5||SGD DESC:GID Complex subunit, serves as adaptor for regulatory subunit Vid24p; protein involved in proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase); localized to the nucleus and the cytoplasm Gene:VMS1(YDR049W)|FD-Score:8.38|P-value:2.75E-17||SGD DESC:Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans Gene:VPS13(YLL040C)|FD-Score:4.82|P-value:7.19E-7||SGD DESC:Protein of unknown function; heterooligomeric or homooligomeric complex; peripherally associated with membranes; involved in sporulation, vacuolar protein sorting, prospore membrane formation and protein-Golgi retention; homologous to human CHAC and COH1 which are involved in chorea acanthocytosis and Cohen syndrome, respectively Gene:VPS17(YOR132W)|FD-Score:6.83|P-value:4.30E-12||SGD DESC:Subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde protein transport; peripheral membrane protein that assembles onto the membrane with Vps5p to promote vesicle formation; required for recruiting the retromer complex to the endosome membranes Gene:VPS24(YKL041W)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); forms an ESCRT-III subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway Gene:VPS25(YJR102C)|FD-Score:5.66|P-value:7.60E-9||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS27(YNR006W)|FD-Score:6.98|P-value:1.45E-12||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:VPS29(YHR012W)|FD-Score:8.6|P-value:3.91E-18||SGD DESC:Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval Gene:VPS3(YDR495C)|FD-Score:6.3|P-value:1.45E-10||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS35(YJL154C)|FD-Score:12.1|P-value:3.45E-34||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:VPS4(YPR173C)|FD-Score:6.53|P-value:3.28E-11||SGD DESC:AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism Gene:VPS41(YDR080W)|FD-Score:8.13|P-value:2.07E-16||SGD DESC:Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport Gene:VPS5(YOR069W)|FD-Score:6.1|P-value:5.35E-10||SGD DESC:Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes Gene:VPS55(YJR044C)|FD-Score:3.18|P-value:7.29E-4||SGD DESC:Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP) Gene:VPS70(YJR126C)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:Protein of unknown function involved in vacuolar protein sorting Gene:VPS75(YNL246W)|FD-Score:5.08|P-value:1.84E-7||SGD DESC:NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress Gene:VPS9(YML097C)|FD-Score:4.77|P-value:9.32E-7||SGD DESC:A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin Gene:WHI3(YNL197C)|FD-Score:4.13|P-value:1.78E-5||SGD DESC:RNA binding protein that sequesters CLN3 mRNA in cytoplasmic foci;; Tpk1 (PKA) mediated phosphorylation of Whi3 affects G1/S transition by modulating Cln3 transcription; cytoplasmic retention factor for Cdc28p and associated cyclins; regulates cell fate and dose-dependently regulates the critical cell size required for passage through Start; WHI3 has a paralog, WHI4, that arose from the whole genome duplication Gene:YBL071C-B(YBL071C-B_p)|FD-Score:4.18|P-value:1.44E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBL071C(YBL071C_d)|FD-Score:5.41|P-value:3.24E-8||SGD DESC:Dubious open reading frame, predicted protein contains a peroxisomal targeting signal Gene:YBP2(YGL060W)|FD-Score:4|P-value:3.11E-5||SGD DESC:Central kinetochore associated protein; mediates mitotic progression; interacts with several central kinetochore proteins and centromeric histone Cse4p; role in resistance to oxidative stress; similar to Slk19p; YBP2 has a paralog, YBP1, that arose from the whole genome duplication Gene:YBR196C-A(YBR196C-A_p)|FD-Score:-4.33|P-value:7.34E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YCR075W-A(YCR075W-A_p)|FD-Score:6|P-value:9.95E-10||SGD DESC:Putative protein of unknown function; identified by homology to Ashbya gossypii; YCR075W-A has a paralog, YNR034W-A, that arose from the whole genome duplication Gene:YDL023C(YDL023C_d)|FD-Score:4.9|P-value:4.74E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in other Saccharomyces species; overlaps the verified gene GPD1; deletion confers sensitivity to GSAO; deletion in cyr1 mutant results in loss of stress resistance Gene:YDL041W(YDL041W_d)|FD-Score:6.83|P-value:4.32E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YDL172C(YDL172C_d)|FD-Score:9.6|P-value:4.12E-22||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL183C(YDL183C)|FD-Score:-4.96|P-value:3.53E-7||SGD DESC:Mitochondrial inner-membrane protein thought to be involved in the formation of an active mitochondrial K+/H+ exchanger (KHE) system; non-essential gene Gene:YDR290W(YDR290W_d)|FD-Score:-3.33|P-value:4.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Gene:YDR417C(YDR417C_d)|FD-Score:3.8|P-value:7.22E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RPL12B/YDR418W Gene:YEN1(YER041W)|FD-Score:-4.09|P-value:2.18E-5||SGD DESC:Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p Gene:YGL042C(YGL042C_d)|FD-Score:4.75|P-value:1.02E-6||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene DST1 Gene:YGL072C(YGL072C_d)|FD-Score:7.34|P-value:1.09E-13||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGL188C-A(YGL188C-A_p)|FD-Score:4.46|P-value:4.10E-6||SGD DESC:Putative protein of unknown function Gene:YGR111W(YGR111W_p)|FD-Score:-3.69|P-value:1.10E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YGR122W(YGR122W)|FD-Score:-4.63|P-value:1.87E-6||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YGR164W(YGR164W_d)|FD-Score:-5.19|P-value:1.04E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIA6(YIL006W)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Mitochondrial NAD+ transporter, involved in the transport of NAD+ into the mitochondria (see also YEA6); member of the mitochondrial carrier subfamily; disputed role as a pyruvate transporter; has putative mouse and human orthologs Gene:YJL193W(YJL193W_p)|FD-Score:-3.19|P-value:7.20E-4||SGD DESC:Putative protein of unknown function, predicted to encode a triose phosphate transporter subfamily member based on phylogenetic analysis; similar to YOR307C/SLY41; deletion mutant has a respiratory growth defect Gene:YKE2(YLR200W)|FD-Score:3.85|P-value:5.95E-5||SGD DESC:Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin Gene:YKL118W(YKL118W_d)|FD-Score:-3.36|P-value:3.84E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YKR018C(YKR018C_p)|FD-Score:-3.52|P-value:2.18E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication Gene:YLR149C(YLR149C_p)|FD-Score:-3.18|P-value:7.40E-4||SGD DESC:Protein of unknown function; overexpression causes a cell cycle delay or arrest; null mutation results in a decrease in plasma membrane electron transport; YLR149C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YLR171W(YLR171W_d)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR224W(YLR224W_p)|FD-Score:3.82|P-value:6.64E-5||SGD DESC:F-box protein and component of SCF ubiquitin ligase complexes involved in ubiquitin-dependent protein catabolism; readily monoubiquitinated in vitro by SCF-Ubc4 complexes; YLR224W is not an essential gene Gene:YLR434C(YLR434C_d)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W Gene:YML094C-A(YML094C-A_d)|FD-Score:4.67|P-value:1.49E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO Gene:YNL120C(YNL120C_d)|FD-Score:3.95|P-value:3.96E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion enhances replication of Brome mosaic virus in S. cerevisiae, but likely due to effects on the overlapping gene Gene:YNL235C(YNL235C_d)|FD-Score:-4.68|P-value:1.43E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex Gene:YNR005C(YNR005C_d)|FD-Score:5.97|P-value:1.19E-9||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL050C(YOL050C_d)|FD-Score:-5.95|P-value:1.36E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YOR1(YGR281W)|FD-Score:4.02|P-value:2.90E-5||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter mediates export of many different organic anions including oligomycin; similar to human cystic fibrosis transmembrane receptor (CFTR) Gene:YOR199W(YOR199W_d)|FD-Score:-3.53|P-value:2.11E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL062W(YPL062W_d)|FD-Score:-3.43|P-value:2.97E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation Gene:YPL102C(YPL102C_d)|FD-Score:8.4|P-value:2.29E-17||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4 Gene:YPR084W(YPR084W_p)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Putative protein of unknown function Gene:YPR123C(YPR123C_d)|FD-Score:-7.82|P-value:2.74E-15||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPR197C(YPR197C_d)|FD-Score:-4.71|P-value:1.23E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPT7(YML001W)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7 Gene:ZAP1(YJL056C)|FD-Score:-4.53|P-value:2.92E-6||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains Gene:ZUO1(YGR285C)|FD-Score:3.12|P-value:9.04E-4||SGD DESC:Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YKL203C17.906.33E-723.45TOR2PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
YHR186C16.904.41E-643.45KOG1Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors
YHR036W15.106.56E-523.45BRL1Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export
YJL156C11.709.35E-320.02SSY5Serine protease of SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); contains an inhibitory domain that dissociates in response to extracellular amino acids, freeing a catalytic domain to activate transcription factor Stp1p
YGL116W11.701.12E-310.10CDC20Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
YIL048W11.603.55E-310.25NEO1Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus
YOR257W11.306.10E-300.80CDC31Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation
YJL054W10.504.13E-260.88TIM54Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane
YDR023W9.623.28E-220.16SES1Cytosolic seryl-tRNA synthetase, class II aminoacyl-tRNA synthetase that aminoacylates tRNA(Ser), displays tRNA-dependent amino acid recognition which enhances discrimination of the serine substrate, interacts with peroxin Pex21p
YER133W9.461.58E-210.16GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YPL237W9.297.47E-210.23SUI3Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding
YPR034W9.066.28E-200.28ARP7Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YLR033W8.787.89E-190.00RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YJR013W8.788.07E-190.82GPI14Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M
YBR049C7.968.48E-160.02REB1RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YCR053W25.605.86E-145THR4Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway
YER052C25.305.45E-141HOM3Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
YDR158W18.101.72E-73HOM2Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
YBR077C17.004.42E-65SLM4Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4
YML121W16.604.26E-62GTR1Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB
YHR187W15.708.27E-56IKI1Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin
YNL084C14.501.09E-47END3EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p
YOR274W14.004.59E-45MOD5Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis
YGR163W13.005.45E-39GTR2Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD
YJR066W12.903.11E-38TOR1PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication
YGL212W12.702.27E-37VAM7Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress
YOR035C12.601.00E-36SHE4Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization
YGR208W12.506.88E-36SER2Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source
YJR139C12.506.93E-36HOM6Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions
YNL119W12.303.44E-35NCS2Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae

GO enrichment analysis for SGTC_2679
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.7610SGTC_1844rapamycin 4.0 nMMiscellaneous52846161
0.2261.87E-69SGTC_163TUB2.036 7.5 μMChemDiv (Drug-like library)159922070.0588235
0.2214.31E-66SGTC_1873rapamycin 2.0 nMMiscellaneous52846161
0.2214.56E-66SGTC_70192-1582 4.2 μMChemDiv (Drug-like library)2526160.0551181
0.1782.06E-43SGTC_1877wortmannin 25.0 μMICCB bioactive library3121450.100671
0.1783.95E-43SGTC_445e6 berbamine 66.0 μMICCB bioactive library66102680.0787879NEO1-PIK1
0.1681.07E-38SGTC_221trifluoperazine 10.3 μMMiscellaneous55660.05NEO1-PIK1
0.1626.59E-36SGTC_3014119-0100 24.5 μMChemDiv (Drug-like library)28010.0447761NEO1
0.1591.32E-34SGTC_232clozapine 119.0 μMMiscellaneous28180.0437956NEO1
0.1511.25E-31SGTC_2651TUB2.036 5.0 μMChemDiv (Drug-like library)159922070.0588235
0.1447.80E-29SGTC_1754st042065 16.1 μMTimTec (Natural product derivative library)11262360.0461538NEO1-PIK1
0.1425.76E-28SGTC_590330-0160 1.3 μMChemDiv (Drug-like library)42934820.0425532
0.1427.69E-28SGTC_2233sulbentine 40.4 μMMiscellaneous676860.0393701
0.1402.85E-27SGTC_1803st050068 52.4 μMTimTec (Natural product derivative library)39840590.0503597NEO1-PIK1
0.1404.33E-27SGTC_180111-0177 500.0 nMChemDiv (Drug-like library)7217520.0512821

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1844rapamycin4 nM15284616Miscellaneous914.171866.295313
SGTC_1873rapamycin2 nM15284616Miscellaneous914.171866.295313
SGTC_233rapamycin362 pM15284616Miscellaneous914.171866.295313
SGTC_1104tacrolimus29.8 μM0.35915523581796NIH Clinical Collection804.018164.832312
SGTC_2689tacrolimus100 μM0.35915523581796NIH Clinical Collection804.018164.832312
SGTC_2645abamectin100 μM0.2181825702208Microsource (Natural product library)873.076884.692314heme requiring
SGTC_466geldanamycin89.2 μM0.1610745288382ICCB bioactive library560.63592.3639RPP1 & pyrimidine depletion
SGTC_1872amphotericin b148 nM0.15923616667679Microsource (Natural product library)924.07902-2.6171218RNA pol III & RNase P/MRP
SGTC_2663amphotericin b100 μM0.15923616667679Microsource (Natural product library)924.07902-2.6171218
SGTC_2665lovastatin100 μM0.15714353232Miscellaneous404.539644.21815
SGTC_504lovastatin124 μM0.15714353232ICCB bioactive library404.539644.21815
SGTC_1545lovastatin49.4 μM0.15714312923612TimTec (Pure natural product library)404.539644.21815
SGTC_1578josamycin24.2 μM0.1485715282165TimTec (Pure natural product library)827.994962.957316
SGTC_2420aureobasidin a50 nM0.1481489963430Miscellaneous1101.419486.007411
SGTC_2567aureobasidin a60 nM0.1481489963430Miscellaneous1101.419486.007411
SGTC_1574spiramycin23.7 μM0.1479296419898TimTec (Pure natural product library)843.052662.049416
SGTC_246cycloheximide667 μM0.14166716760040ICCB bioactive library281.347420.93324