piperine

(2E,4E)-5-(1,3-benzodioxol-5-yl)-1-piperidin-1-ylpenta-2,4-dien-1-one

Piperine is an alkaloid from black pepper that shows chemopreventive, cytotoxic, anti-inflammatory, and antioxidant effects. It is suggested that piperine exerts its chemopreventive effect by augmenting antioxidant defense system and modulating lipid peroxidation.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2683
Screen concentration 63.0 μM
Source TimTec (Pure natural product library)
PubChem CID 638024
SMILES C1CCN(CC1)C(=O)C=CC=CC2=CC3=C(C=C2)OCO3
Standardized SMILES O=C(C=CC=Cc1ccc2OCOc2c1)N3CCCCC3
Molecular weight 285.3377
ALogP 2.86
H-bond donor count 0
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 16.88
% growth inhibition (Hom. pool) 12.29


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 638024
Download HIP data (tab-delimited text)  (excel)
Gene:MOT1(YPL082C)|FD-Score:3.33|P-value:4.34E-4|Clearance:0.1||SGD DESC:Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA Gene:NTF2(YER009W)|FD-Score:3.19|P-value:7.09E-4|Clearance:0.18||SGD DESC:Nuclear envelope protein, interacts with GDP-bound Gsp1p and with proteins of the nuclear pore to transport Gsp1p into the nucleus where it is an essential player in nucleocytoplasmic transport Gene:RFT1(YBL020W)|FD-Score:3.23|P-value:6.15E-4|Clearance:0.04||SGD DESC:Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein Gene:RPF2(YKR081C)|FD-Score:3.48|P-value:2.54E-4|Clearance:0.15||SGD DESC:Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles Gene:SAM50(YNL026W)|FD-Score:-3.17|P-value:7.57E-4|Clearance:0||SGD DESC:Essential component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; homologous to bacterial Omp85 Gene:SCM3(YDL139C)|FD-Score:3.19|P-value:7.09E-4|Clearance:0||SGD DESC:Nonhistone component of centromeric chromatin; binds stoichiometrically to CenH3-H4 histones, required for kinetochore assembly; required for G2/M progression and localization of Cse4p; may protect Cse4p from ubiquitylation; homolog of mammalian HJURP Gene:YCL041C(YCL041C_d)|FD-Score:3.87|P-value:5.39E-5|Clearance:0.4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps both the verified gene PDI1/YCL043C and the uncharacterized gene YCL042W Gene:MOT1(YPL082C)|FD-Score:3.33|P-value:4.34E-4|Clearance:0.1||SGD DESC:Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA Gene:NTF2(YER009W)|FD-Score:3.19|P-value:7.09E-4|Clearance:0.18||SGD DESC:Nuclear envelope protein, interacts with GDP-bound Gsp1p and with proteins of the nuclear pore to transport Gsp1p into the nucleus where it is an essential player in nucleocytoplasmic transport Gene:RFT1(YBL020W)|FD-Score:3.23|P-value:6.15E-4|Clearance:0.04||SGD DESC:Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein Gene:RPF2(YKR081C)|FD-Score:3.48|P-value:2.54E-4|Clearance:0.15||SGD DESC:Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles Gene:SAM50(YNL026W)|FD-Score:-3.17|P-value:7.57E-4|Clearance:0||SGD DESC:Essential component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; homologous to bacterial Omp85 Gene:SCM3(YDL139C)|FD-Score:3.19|P-value:7.09E-4|Clearance:0||SGD DESC:Nonhistone component of centromeric chromatin; binds stoichiometrically to CenH3-H4 histones, required for kinetochore assembly; required for G2/M progression and localization of Cse4p; may protect Cse4p from ubiquitylation; homolog of mammalian HJURP Gene:YCL041C(YCL041C_d)|FD-Score:3.87|P-value:5.39E-5|Clearance:0.4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps both the verified gene PDI1/YCL043C and the uncharacterized gene YCL042W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 638024
Download HOP data (tab-delimited text)  (excel)
Gene:ADH6(YMR318C)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:NADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance; protein abundance increases in response to DNA replication stress Gene:ADP1(YCR011C)|FD-Score:4.08|P-value:2.30E-5||SGD DESC:Putative ATP-dependent permease of the ABC transporter family of proteins Gene:ASG7(YJL170C)|FD-Score:3.13|P-value:8.82E-4||SGD DESC:Protein that regulates signaling from a G protein beta subunit Ste4p and its relocalization within the cell; specific to a-cells and induced by alpha-factor Gene:BNA6(YFR047C)|FD-Score:4.5|P-value:3.34E-6||SGD DESC:Quinolinate phosphoribosyl transferase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:CBC2(YPL178W)|FD-Score:-3.38|P-value:3.67E-4||SGD DESC:Small subunit of the heterodimeric cap binding complex that also contains Sto1p, component of the spliceosomal commitment complex; interacts with Npl3p, possibly to package mRNA for export from the nucleus; contains an RNA-binding motif Gene:CCW12(YLR110C)|FD-Score:4.19|P-value:1.42E-5||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CDC26(YFR036W)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:COG5(YNL051W)|FD-Score:4.18|P-value:1.47E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DAL81(YIR023W)|FD-Score:9.81|P-value:4.90E-23||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:FMP48(YGR052W_p)|FD-Score:-3.14|P-value:8.59E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:GCN2(YDR283C)|FD-Score:3.1|P-value:9.82E-4||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GSM1(YJL103C)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Putative zinc cluster protein of unknown function; proposed to be involved in the regulation of energy metabolism, based on patterns of expression and sequence analysis Gene:HPC2(YBR215W)|FD-Score:-3.7|P-value:1.09E-4||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; mutants display synthetic defects with subunits of FACT, a complex that allows passage of RNA Pol II through nucleosomes Gene:JJJ3(YJR097W)|FD-Score:3.25|P-value:5.75E-4||SGD DESC:Protein of unknown function, contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4 Gene:KTR2(YKR061W)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Mannosyltransferase involved in N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR2 has a paralog, YUR1, that arose from the whole genome duplication Gene:MDM36(YPR083W)|FD-Score:-3.53|P-value:2.09E-4||SGD DESC:Mitochondrial protein required for normal mitochondrial morphology and inheritance; proposed involvement in the formation of Dnm1p and Num1p-containing cortical anchor complexes that promote mitochondrial fission Gene:MNT2(YGL257C)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:Mannosyltransferase involved in adding the 4th and 5th mannose residues of O-linked glycans Gene:MSN1(YOL116W)|FD-Score:4.06|P-value:2.46E-5||SGD DESC:Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:NGG1(YDR176W)|FD-Score:4.67|P-value:1.52E-6||SGD DESC:Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex Gene:RAM1(YDL090C)|FD-Score:3.92|P-value:4.38E-5||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RFM1(YOR279C)|FD-Score:4.27|P-value:9.62E-6||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RHR2(YIL053W)|FD-Score:-3.56|P-value:1.85E-4||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:RPL6B(YLR448W)|FD-Score:3.29|P-value:5.04E-4||SGD DESC:Ribosomal 60S subunit protein L6B; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6B has a paralog, RPL6A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS29A(YLR388W)|FD-Score:3.99|P-value:3.35E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication Gene:SQS1(YNL224C)|FD-Score:3.31|P-value:4.65E-4||SGD DESC:Protein that stimulates the ATPase and helicase activities of Prp43p; acts with Prp43p to stimulate 18s rRNA maturation by Nob1p; overexpression antagonizes the suppression of splicing defects by spp382 mutants; component of pre-ribosomal particles; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:SUR2(YDR297W)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis Gene:SUR4(YLR372W)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SWI4(YER111C)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p Gene:SWR1(YDR334W)|FD-Score:-3.54|P-value:2.02E-4||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:SYS1(YJL004C)|FD-Score:4.46|P-value:4.07E-6||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TRP1(YDR007W)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:7.99|P-value:6.63E-16||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:10.3|P-value:2.56E-25||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:6.12|P-value:4.76E-10||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:YDR008C(YDR008C_d)|FD-Score:8.47|P-value:1.26E-17||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR541C(YDR541C_p)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Putative dihydrokaempferol 4-reductase Gene:YER156C(YER156C_p)|FD-Score:3.29|P-value:5.06E-4||SGD DESC:Putative protein of unknown function; interacts with Hsp82p and copurifies with Ipl1p; expression is copper responsive and downregulated in strains deleted for MAC1, a copper-responsive transcription factor; similarity to mammalian MYG1 Gene:YER186C(YER186C_p)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Putative protein of unknown function Gene:YFL032W(YFL032W_d)|FD-Score:4.43|P-value:4.71E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene Gene:YFR045W(YFR045W_p)|FD-Score:3.28|P-value:5.19E-4||SGD DESC:Putative mitochondrial transport protein; null mutant is viable, exhibits decreased levels of chitin and normal resistance to calcofluor white Gene:YIL102C(YIL102C_p)|FD-Score:-3.23|P-value:6.13E-4||SGD DESC:Putative protein of unknown function Gene:YKL071W(YKL071W_p)|FD-Score:4.41|P-value:5.27E-6||SGD DESC:Putative protein of unknown function; expression induced in cells treated with the mycotoxin patulin, and also the quinone methide triterpene celastrol; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YPR053C(YPR053C_d)|FD-Score:3.25|P-value:5.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene NHP6A/YPR052C Gene:ADH6(YMR318C)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:NADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance; protein abundance increases in response to DNA replication stress Gene:ADP1(YCR011C)|FD-Score:4.08|P-value:2.30E-5||SGD DESC:Putative ATP-dependent permease of the ABC transporter family of proteins Gene:ASG7(YJL170C)|FD-Score:3.13|P-value:8.82E-4||SGD DESC:Protein that regulates signaling from a G protein beta subunit Ste4p and its relocalization within the cell; specific to a-cells and induced by alpha-factor Gene:BNA6(YFR047C)|FD-Score:4.5|P-value:3.34E-6||SGD DESC:Quinolinate phosphoribosyl transferase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:CBC2(YPL178W)|FD-Score:-3.38|P-value:3.67E-4||SGD DESC:Small subunit of the heterodimeric cap binding complex that also contains Sto1p, component of the spliceosomal commitment complex; interacts with Npl3p, possibly to package mRNA for export from the nucleus; contains an RNA-binding motif Gene:CCW12(YLR110C)|FD-Score:4.19|P-value:1.42E-5||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CDC26(YFR036W)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:COG5(YNL051W)|FD-Score:4.18|P-value:1.47E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DAL81(YIR023W)|FD-Score:9.81|P-value:4.90E-23||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:FMP48(YGR052W_p)|FD-Score:-3.14|P-value:8.59E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:GCN2(YDR283C)|FD-Score:3.1|P-value:9.82E-4||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GSM1(YJL103C)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Putative zinc cluster protein of unknown function; proposed to be involved in the regulation of energy metabolism, based on patterns of expression and sequence analysis Gene:HPC2(YBR215W)|FD-Score:-3.7|P-value:1.09E-4||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; mutants display synthetic defects with subunits of FACT, a complex that allows passage of RNA Pol II through nucleosomes Gene:JJJ3(YJR097W)|FD-Score:3.25|P-value:5.75E-4||SGD DESC:Protein of unknown function, contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4 Gene:KTR2(YKR061W)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Mannosyltransferase involved in N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR2 has a paralog, YUR1, that arose from the whole genome duplication Gene:MDM36(YPR083W)|FD-Score:-3.53|P-value:2.09E-4||SGD DESC:Mitochondrial protein required for normal mitochondrial morphology and inheritance; proposed involvement in the formation of Dnm1p and Num1p-containing cortical anchor complexes that promote mitochondrial fission Gene:MNT2(YGL257C)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:Mannosyltransferase involved in adding the 4th and 5th mannose residues of O-linked glycans Gene:MSN1(YOL116W)|FD-Score:4.06|P-value:2.46E-5||SGD DESC:Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:NGG1(YDR176W)|FD-Score:4.67|P-value:1.52E-6||SGD DESC:Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex Gene:RAM1(YDL090C)|FD-Score:3.92|P-value:4.38E-5||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RFM1(YOR279C)|FD-Score:4.27|P-value:9.62E-6||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RHR2(YIL053W)|FD-Score:-3.56|P-value:1.85E-4||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:RPL6B(YLR448W)|FD-Score:3.29|P-value:5.04E-4||SGD DESC:Ribosomal 60S subunit protein L6B; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6B has a paralog, RPL6A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS29A(YLR388W)|FD-Score:3.99|P-value:3.35E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication Gene:SQS1(YNL224C)|FD-Score:3.31|P-value:4.65E-4||SGD DESC:Protein that stimulates the ATPase and helicase activities of Prp43p; acts with Prp43p to stimulate 18s rRNA maturation by Nob1p; overexpression antagonizes the suppression of splicing defects by spp382 mutants; component of pre-ribosomal particles; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:SUR2(YDR297W)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis Gene:SUR4(YLR372W)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SWI4(YER111C)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p Gene:SWR1(YDR334W)|FD-Score:-3.54|P-value:2.02E-4||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:SYS1(YJL004C)|FD-Score:4.46|P-value:4.07E-6||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TRP1(YDR007W)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:7.99|P-value:6.63E-16||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:10.3|P-value:2.56E-25||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:6.12|P-value:4.76E-10||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:YDR008C(YDR008C_d)|FD-Score:8.47|P-value:1.26E-17||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR541C(YDR541C_p)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Putative dihydrokaempferol 4-reductase Gene:YER156C(YER156C_p)|FD-Score:3.29|P-value:5.06E-4||SGD DESC:Putative protein of unknown function; interacts with Hsp82p and copurifies with Ipl1p; expression is copper responsive and downregulated in strains deleted for MAC1, a copper-responsive transcription factor; similarity to mammalian MYG1 Gene:YER186C(YER186C_p)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Putative protein of unknown function Gene:YFL032W(YFL032W_d)|FD-Score:4.43|P-value:4.71E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene Gene:YFR045W(YFR045W_p)|FD-Score:3.28|P-value:5.19E-4||SGD DESC:Putative mitochondrial transport protein; null mutant is viable, exhibits decreased levels of chitin and normal resistance to calcofluor white Gene:YIL102C(YIL102C_p)|FD-Score:-3.23|P-value:6.13E-4||SGD DESC:Putative protein of unknown function Gene:YKL071W(YKL071W_p)|FD-Score:4.41|P-value:5.27E-6||SGD DESC:Putative protein of unknown function; expression induced in cells treated with the mycotoxin patulin, and also the quinone methide triterpene celastrol; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YPR053C(YPR053C_d)|FD-Score:3.25|P-value:5.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene NHP6A/YPR052C

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YCL041C_d3.875.39E-50.40YCL041C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps both the verified gene PDI1/YCL043C and the uncharacterized gene YCL042W
YKR081C3.482.54E-40.15RPF2Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles
YPL082C3.334.34E-40.10MOT1Essential protein involved in regulation of transcription; removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity; the Mot1p-Spt15p-DNA ternary complex contains unbent DNA
YBL020W3.236.15E-40.04RFT1Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein
YDL139C3.197.09E-41.17E-4SCM3Nonhistone component of centromeric chromatin; binds stoichiometrically to CenH3-H4 histones, required for kinetochore assembly; required for G2/M progression and localization of Cse4p; may protect Cse4p from ubiquitylation; homolog of mammalian HJURP
YER009W3.197.09E-40.17NTF2Nuclear envelope protein, interacts with GDP-bound Gsp1p and with proteins of the nuclear pore to transport Gsp1p into the nucleus where it is an essential player in nucleocytoplasmic transport
YGR251W3.020.001280.04NOP19Ribosome biogenesis factor; nucleolar protein associated with pre-rRNA components of the 90S preribosome, required for cleavage of pre-rRNA at A0, A1 and A2 sites; interacts with RNA helicase Dhr2p and RNA helicase-like protein Utp25p; required for incorporation of Utp25p into preribosomes
YGR098C2.970.001480.06ESP1Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress
YGL055W2.910.001810.03OLE1Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
YEL019C2.880.002000.03MMS21SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination
YLR310C2.850.002200.02CDC25Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1
YNL152W2.830.002340.07INN1Essential protein that associates with contractile actomyosin ring; required for ingression of the plasma membrane into the bud neck during cytokinesis; C2 domain, a membrane targeting module, is required for function; activates chitin synthase activity of Chs2p during cytokinesis
YLR230W_d2.750.002940.16YLR230W_dDubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity
YGL099W2.590.004770.05LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YGL150C2.540.005530.01INO80ATPase and nucleosome spacing factor, subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; has a role in modulating stress gene transcription

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKL211C10.302.56E-25TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YIR023W9.814.90E-23DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YDR008C_d8.471.26E-17YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YER090W7.996.63E-16TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YDR354W6.124.76E-10TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YDR176W4.671.52E-6NGG1Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex
YFR047C4.503.34E-6BNA6Quinolinate phosphoribosyl transferase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p
YJL004C4.464.07E-6SYS1Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation
YFL032W_d4.434.71E-6YFL032W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene
YKL071W_p4.415.27E-6YKL071W_pPutative protein of unknown function; expression induced in cells treated with the mycotoxin patulin, and also the quinone methide triterpene celastrol; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
YOR279C4.279.62E-6RFM1DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance
YLR110C4.191.42E-5CCW12Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication
YNL051W4.181.47E-5COG5Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YFR036W4.161.61E-5CDC26Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
YCR011C4.082.30E-5ADP1Putative ATP-dependent permease of the ABC transporter family of proteins

GO enrichment analysis for SGTC_2683
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.4503.77E-292SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.0454545
0.4197.95E-250SGTC_20935331342 194.4 μMChembridge (Fragment library)338390.107692
0.4152.91E-245SGTC_21976588183 93.1 μMChembridge (Fragment library)8841360.111111
0.4102.40E-238SGTC_2618avocadyne 43.4 μMMicrosource (Natural product library)3015189NA
0.3971.18E-221SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.0517241
0.3902.96E-213SGTC_21375261020 140.0 μMChembridge (Fragment library)7636550.0869565
0.3684.62E-189SGTC_1824st053723 68.6 μMTimTec (Natural product derivative library)6752250.106061
0.3633.63E-183SGTC_14964469-0713 57.4 μMChemDiv (Drug-like library)7466910.0375
0.3601.79E-180SGTC_1699st031318 29.1 μMTimTec (Natural product derivative library)53406580.190476
0.3516.33E-171SGTC_15412',3',6-trimethoxyflavone 64.0 μMTimTec (Pure natural product library)6888010.112676
0.3501.52E-169SGTC_22547938975 95.2 μMChembridge (Fragment library)29692770.0571429
0.3491.21E-168SGTC_22246650123 117.6 μMChembridge (Fragment library)29083700.0958904
0.3441.03E-163SGTC_29399044578 71.4 μMChembridge (Drug-like library)26536340.126582
0.3416.36E-161SGTC_1975st070967 30.8 μMTimTec (Natural product derivative library)2613910.0952381
0.3401.14E-159SGTC_1801486-1293 102.8 μMChemDiv (Drug-like library)41405360.0875

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1594piperine70.1 μM1638024TimTec (Pure natural product library)285.337662.86403
SGTC_2587piperic acid92.2 μM0.6363645370536Microsource (Natural product library)218.20542.16314
SGTC_7991310-0557151 μM0.4897965354492ChemDiv (Drug-like library)252.264683.4703superoxide
SGTC_1837st05547266.5 μM0.45614676091TimTec (Natural product derivative library)301.380123.68703
SGTC_1795st04954562.3 μM0.4259265291882TimTec (Natural product derivative library)321.15484.79803TSC3-RPN4
SGTC_14404100-155040 μM0.421053742806ChemDiv (Drug-like library)268.267382.23914
SGTC_13661683-011321.9 μM0.42708623ChemDiv (Drug-like library)224.233121.75925
SGTC_1689st02026553.49 μM0.4137935758320TimTec (Natural product derivative library)336.38443.40405
SGTC_1710st03175861.5 μM0.3870975394341TimTec (Natural product derivative library)325.31542.70515
SGTC_1845st0567533.01 μM0.3846155375839TimTec (Natural product derivative library)207.182762.09104TSC3-RPN4