mycophenolic acid FDA approved compound

(E)-6-(4-hydroxy-6-methoxy-7-methyl-3-oxo-1H-2-benzofuran-5-yl)-4-methylhex-4-enoic acid

Mycophenolic acid is a potent reversible inhibitor of IMPDH (inosine monophosphate dehydrogenase), leading to a depletion of GMP and inhibition of de novo synthesis of purines. The resulting imbalance of nucleotide pools also results in inhibiting transcription-elongation.

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PubChem MeSH terms: Antibiotics, Antineoplastic;Enzyme Inhibitors



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2685
Screen concentration 18.9 μM
Source Miscellaneous
PubChem CID 446541
SMILES CC1=C(C(=C(C2=C1COC2=O)O)CC=C(C)CCC(=O)O)OC
Standardized SMILES COc1c(C)c2COC(=O)c2c(O)c1CC=C(C)CCC(=O)O
Molecular weight 320.3371
ALogP 3.16
H-bond donor count 2
H-bond acceptor count 6
Response signature RNA pol III & RNase P/MRP

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.35
% growth inhibition (Hom. pool) 5.43


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 446541
Download HIP data (tab-delimited text)  (excel)
Gene:CFT1(YDR301W)|FD-Score:-3.3|P-value:4.83E-4|Clearance:0||SGD DESC:RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF Gene:IPI1(YHR085W)|FD-Score:3.28|P-value:5.18E-4|Clearance:0.47||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; IPI1 is an essential gene Gene:IRA1(YBR140C)|FD-Score:-4.14|P-value:1.74E-5|Clearance:0||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:POP1(YNL221C)|FD-Score:9.05|P-value:6.88E-20|Clearance:3.46||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:RPB8(YOR224C)|FD-Score:4.16|P-value:1.62E-5|Clearance:0.88||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPC53(YDL150W)|FD-Score:4.91|P-value:4.53E-7|Clearance:0.76||SGD DESC:RNA polymerase III subunit C53 Gene:RPO31(YOR116C)|FD-Score:5.59|P-value:1.13E-8|Clearance:0.68||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:RPP1(YHR062C)|FD-Score:10.9|P-value:9.38E-28|Clearance:3.46||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:TOM40(YMR203W)|FD-Score:-3.1|P-value:9.76E-4|Clearance:0||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:CFT1(YDR301W)|FD-Score:-3.3|P-value:4.83E-4|Clearance:0||SGD DESC:RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF Gene:IPI1(YHR085W)|FD-Score:3.28|P-value:5.18E-4|Clearance:0.47||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; IPI1 is an essential gene Gene:IRA1(YBR140C)|FD-Score:-4.14|P-value:1.74E-5|Clearance:0||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:POP1(YNL221C)|FD-Score:9.05|P-value:6.88E-20|Clearance:3.46||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:RPB8(YOR224C)|FD-Score:4.16|P-value:1.62E-5|Clearance:0.88||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPC53(YDL150W)|FD-Score:4.91|P-value:4.53E-7|Clearance:0.76||SGD DESC:RNA polymerase III subunit C53 Gene:RPO31(YOR116C)|FD-Score:5.59|P-value:1.13E-8|Clearance:0.68||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:RPP1(YHR062C)|FD-Score:10.9|P-value:9.38E-28|Clearance:3.46||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:TOM40(YMR203W)|FD-Score:-3.1|P-value:9.76E-4|Clearance:0||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 446541
Download HOP data (tab-delimited text)  (excel)
Gene:ABF2(YMR072W)|FD-Score:-3.13|P-value:8.83E-4||SGD DESC:Mitochondrial DNA-binding protein involved in mitochondrial DNA replication and recombination, member of HMG1 DNA-binding protein family; activity may be regulated by protein kinase A phosphorylation Gene:ADK1(YDR226W)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress Gene:AIM2(YAL049C)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Cytoplasmic protein involved in mitochondrial function or organization; null mutant displays reduced frequency of mitochondrial genome loss; potential Hsp82p interactor Gene:COG8(YML071C)|FD-Score:3.2|P-value:6.98E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DNL4(YOR005C)|FD-Score:3.28|P-value:5.20E-4||SGD DESC:DNA ligase required for nonhomologous end-joining (NHEJ), forms stable heterodimer with required cofactor Lif1p, interacts with Nej1p; involved in meiosis, not essential for vegetative growth Gene:DYN3(YMR299C)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration Gene:ECM19(YLR390W)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:EFM1(YHL039W)|FD-Score:3.09|P-value:9.94E-4||SGD DESC:Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:ERC1(YHR032W)|FD-Score:3.4|P-value:3.36E-4||SGD DESC:Member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily; overproduction confers ethionine resistance and accumulation of S-adenosylmethionine Gene:FLR1(YBR008C)|FD-Score:3.21|P-value:6.69E-4||SGD DESC:Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; involved in efflux of fluconazole, diazaborine, benomyl, methotrexate, and other drugs; expression induced in cells treated with the mycotoxin patulin; relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:FRT1(YOR324C)|FD-Score:4.2|P-value:1.34E-5||SGD DESC:Tail-anchored ER membrane protein of unknown function; substrate of the phosphatase calcineurin; interacts with homolog Frt2p; promotes cell growth in stress conditions, possibly via a role in posttranslational translocation; FRT1 has a paralog, FRT2, that arose from the whole genome duplication Gene:HBT1(YDL223C)|FD-Score:4.53|P-value:3.02E-6||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:HIS7(YBR248C)|FD-Score:3.4|P-value:3.36E-4||SGD DESC:Imidazole glycerol phosphate synthase (glutamine amidotransferase:cyclase), catalyzes the fifth step of histidine biosynthesis and also produces 5-aminoimidazole-4-carboxamide ribotide (AICAR), a purine precursor Gene:HOM3(YER052C)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:IMH1(YLR309C)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Protein involved in vesicular transport, mediates transport between an endosomal compartment and the Golgi, contains a Golgi-localization (GRIP) domain that interacts with activated Arl1p-GTP to localize Imh1p to the Golgi Gene:IOC4(YMR044W)|FD-Score:3.18|P-value:7.41E-4||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing Gene:LCB5(YLR260W)|FD-Score:-3.21|P-value:6.72E-4||SGD DESC:Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules Gene:MIP6(YHR015W)|FD-Score:3.14|P-value:8.47E-4||SGD DESC:Putative RNA-binding protein; interacts with Mex67p, which is a component of the nuclear pore involved in nuclear mRNA export; MIP6 has a paralog, PES4, that arose from the whole genome duplication Gene:MRPS35(YGR165W)|FD-Score:-3.3|P-value:4.92E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets Gene:NGL2(YMR285C)|FD-Score:3.15|P-value:8.23E-4||SGD DESC:Protein involved in 5.8S rRNA processing; Ccr4p-like RNase required for correct 3'-end formation of 5.8S rRNA at site E; similar to Ngl1p; NGL2 has a paralog, NGL3, that arose from the whole genome duplication Gene:PEP7(YDR323C)|FD-Score:3.92|P-value:4.45E-5||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PHO80(YOL001W)|FD-Score:3.4|P-value:3.43E-4||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling Gene:SEC22(YLR268W)|FD-Score:-3.69|P-value:1.13E-4||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SPT3(YDR392W)|FD-Score:5.48|P-value:2.09E-8||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:THI12(YNL332W)|FD-Score:3.34|P-value:4.14E-4||SGD DESC:Protein involved in synthesis of the thiamine precursor hydroxymethylpyrimidine (HMP); member of a subtelomeric gene family including THI5, THI11, THI12, and THI13 Gene:THI74(YDR438W)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:Mitochondrial transporter repressible by thiamine; THI74 has a paralog, YML018C, that arose from the whole genome duplication Gene:UBP1(YDL122W)|FD-Score:3.43|P-value:3.01E-4||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains Gene:VHS2(YIL135C)|FD-Score:3.13|P-value:8.88E-4||SGD DESC:Cytoplasmic protein of unknown function; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; similar to Mlf3p Gene:VPS21(YOR089C)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:YBR220C(YBR220C_p)|FD-Score:-3.1|P-value:9.59E-4||SGD DESC:Putative protein of unknown function; YBR220C is not an essential gene Gene:YGL108C(YGL108C_p)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Protein of unknown function, predicted to be palmitoylated; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress Gene:YGR018C(YGR018C_p)|FD-Score:3.93|P-value:4.20E-5||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the uncharacterized ORF YGR017W Gene:YLR412C-A(YLR412C-A_p)|FD-Score:-3.2|P-value:6.99E-4||SGD DESC:Putative protein of unknown function Gene:YML089C(YML089C_d)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression induced by calcium shortage Gene:YPL197C(YPL197C_d)|FD-Score:3.11|P-value:9.24E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the ribosomal gene RPB7B Gene:ABF2(YMR072W)|FD-Score:-3.13|P-value:8.83E-4||SGD DESC:Mitochondrial DNA-binding protein involved in mitochondrial DNA replication and recombination, member of HMG1 DNA-binding protein family; activity may be regulated by protein kinase A phosphorylation Gene:ADK1(YDR226W)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress Gene:AIM2(YAL049C)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Cytoplasmic protein involved in mitochondrial function or organization; null mutant displays reduced frequency of mitochondrial genome loss; potential Hsp82p interactor Gene:COG8(YML071C)|FD-Score:3.2|P-value:6.98E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DNL4(YOR005C)|FD-Score:3.28|P-value:5.20E-4||SGD DESC:DNA ligase required for nonhomologous end-joining (NHEJ), forms stable heterodimer with required cofactor Lif1p, interacts with Nej1p; involved in meiosis, not essential for vegetative growth Gene:DYN3(YMR299C)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration Gene:ECM19(YLR390W)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:EFM1(YHL039W)|FD-Score:3.09|P-value:9.94E-4||SGD DESC:Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:ERC1(YHR032W)|FD-Score:3.4|P-value:3.36E-4||SGD DESC:Member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily; overproduction confers ethionine resistance and accumulation of S-adenosylmethionine Gene:FLR1(YBR008C)|FD-Score:3.21|P-value:6.69E-4||SGD DESC:Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; involved in efflux of fluconazole, diazaborine, benomyl, methotrexate, and other drugs; expression induced in cells treated with the mycotoxin patulin; relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:FRT1(YOR324C)|FD-Score:4.2|P-value:1.34E-5||SGD DESC:Tail-anchored ER membrane protein of unknown function; substrate of the phosphatase calcineurin; interacts with homolog Frt2p; promotes cell growth in stress conditions, possibly via a role in posttranslational translocation; FRT1 has a paralog, FRT2, that arose from the whole genome duplication Gene:HBT1(YDL223C)|FD-Score:4.53|P-value:3.02E-6||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:HIS7(YBR248C)|FD-Score:3.4|P-value:3.36E-4||SGD DESC:Imidazole glycerol phosphate synthase (glutamine amidotransferase:cyclase), catalyzes the fifth step of histidine biosynthesis and also produces 5-aminoimidazole-4-carboxamide ribotide (AICAR), a purine precursor Gene:HOM3(YER052C)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:IMH1(YLR309C)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Protein involved in vesicular transport, mediates transport between an endosomal compartment and the Golgi, contains a Golgi-localization (GRIP) domain that interacts with activated Arl1p-GTP to localize Imh1p to the Golgi Gene:IOC4(YMR044W)|FD-Score:3.18|P-value:7.41E-4||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing Gene:LCB5(YLR260W)|FD-Score:-3.21|P-value:6.72E-4||SGD DESC:Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules Gene:MIP6(YHR015W)|FD-Score:3.14|P-value:8.47E-4||SGD DESC:Putative RNA-binding protein; interacts with Mex67p, which is a component of the nuclear pore involved in nuclear mRNA export; MIP6 has a paralog, PES4, that arose from the whole genome duplication Gene:MRPS35(YGR165W)|FD-Score:-3.3|P-value:4.92E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets Gene:NGL2(YMR285C)|FD-Score:3.15|P-value:8.23E-4||SGD DESC:Protein involved in 5.8S rRNA processing; Ccr4p-like RNase required for correct 3'-end formation of 5.8S rRNA at site E; similar to Ngl1p; NGL2 has a paralog, NGL3, that arose from the whole genome duplication Gene:PEP7(YDR323C)|FD-Score:3.92|P-value:4.45E-5||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PHO80(YOL001W)|FD-Score:3.4|P-value:3.43E-4||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling Gene:SEC22(YLR268W)|FD-Score:-3.69|P-value:1.13E-4||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SPT3(YDR392W)|FD-Score:5.48|P-value:2.09E-8||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:THI12(YNL332W)|FD-Score:3.34|P-value:4.14E-4||SGD DESC:Protein involved in synthesis of the thiamine precursor hydroxymethylpyrimidine (HMP); member of a subtelomeric gene family including THI5, THI11, THI12, and THI13 Gene:THI74(YDR438W)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:Mitochondrial transporter repressible by thiamine; THI74 has a paralog, YML018C, that arose from the whole genome duplication Gene:UBP1(YDL122W)|FD-Score:3.43|P-value:3.01E-4||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains Gene:VHS2(YIL135C)|FD-Score:3.13|P-value:8.88E-4||SGD DESC:Cytoplasmic protein of unknown function; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; similar to Mlf3p Gene:VPS21(YOR089C)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:YBR220C(YBR220C_p)|FD-Score:-3.1|P-value:9.59E-4||SGD DESC:Putative protein of unknown function; YBR220C is not an essential gene Gene:YGL108C(YGL108C_p)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Protein of unknown function, predicted to be palmitoylated; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress Gene:YGR018C(YGR018C_p)|FD-Score:3.93|P-value:4.20E-5||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the uncharacterized ORF YGR017W Gene:YLR412C-A(YLR412C-A_p)|FD-Score:-3.2|P-value:6.99E-4||SGD DESC:Putative protein of unknown function Gene:YML089C(YML089C_d)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression induced by calcium shortage Gene:YPL197C(YPL197C_d)|FD-Score:3.11|P-value:9.24E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the ribosomal gene RPB7B

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR062C10.909.38E-283.46RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YNL221C9.056.88E-203.46POP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
YOR116C5.591.13E-80.68RPO31RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
YDL150W4.914.53E-70.76RPC53RNA polymerase III subunit C53
YOR224C4.161.62E-50.88RPB8RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III
YHR085W3.285.18E-40.47IPI1Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; IPI1 is an essential gene
YOR257W2.810.002480.16CDC31Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation
YJL019W2.650.004050.03MPS3Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p
YLR022C2.620.004390.14SDO1Essential protein involved in ribosome maturation; with Ria1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; ortholog of the human protein (SBDS) responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome; highly conserved across archaea and eukaryotes
YPR186C2.480.006570.02PZF1Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA
YFR004W2.460.006870.05RPN11Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress
YDL152W_d2.420.007860.01YDL152W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome
YGR172C2.410.007980.01YIP1Integral membrane protein required for the biogenesis of ER-derived COPII transport vesicles; interacts with Yif1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF4
YOR261C2.400.008090.01RPN8Essential, non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p
YDL153C2.390.008330.02SAS10Essential subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit; disrupts silencing when overproduced; mutant has increased aneuploidy tolerance

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR392W5.482.09E-8SPT3Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters
YDL223C4.533.02E-6HBT1Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis
YOR324C4.201.34E-5FRT1Tail-anchored ER membrane protein of unknown function; substrate of the phosphatase calcineurin; interacts with homolog Frt2p; promotes cell growth in stress conditions, possibly via a role in posttranslational translocation; FRT1 has a paralog, FRT2, that arose from the whole genome duplication
YGR018C_p3.934.20E-5YGR018C_pProtein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the uncharacterized ORF YGR017W
YDR323C3.924.45E-5PEP7Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance
YLR390W3.641.34E-4ECM19Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YER052C3.631.39E-4HOM3Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
YGL108C_p3.621.49E-4YGL108C_pProtein of unknown function, predicted to be palmitoylated; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress
YLR309C3.611.51E-4IMH1Protein involved in vesicular transport, mediates transport between an endosomal compartment and the Golgi, contains a Golgi-localization (GRIP) domain that interacts with activated Arl1p-GTP to localize Imh1p to the Golgi
YOR089C3.601.58E-4VPS21Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication
YML089C_d3.542.03E-4YML089C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression induced by calcium shortage
YDL122W3.433.01E-4UBP1Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains
YBR248C3.403.36E-4HIS7Imidazole glycerol phosphate synthase (glutamine amidotransferase:cyclase), catalyzes the fifth step of histidine biosynthesis and also produces 5-aminoimidazole-4-carboxamide ribotide (AICAR), a purine precursor
YHR032W3.403.36E-4ERC1Member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily; overproduction confers ethionine resistance and accumulation of S-adenosylmethionine
YOL001W3.403.43E-4PHO80Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling

GO enrichment analysis for SGTC_2685
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2662.64E-96SGTC_2411st077133 39.9 μMTimTec (Natural product derivative library)14031420.151899RNA pol III & RNase P/MRP
0.2604.32E-92SGTC_1872amphotericin b 148.0 nMMicrosource (Natural product library)166676790.0940171RNA pol III & RNase P/MRP
0.2553.55E-88SGTC_919tnp00227 403.6 nMTimTec (Natural product library)12692460.17284RNA pol III & RNase P/MRP
0.2464.00E-82SGTC_265mycophenolic acid 32.9 μMMiscellaneous4465411RPP1 & pyrimidine depletion
0.2281.75E-70SGTC_15346-azauridine 81.6 μMTimTec (Pure natural product library)2335020.0769231RNA pol III & RNase P/MRP
0.2042.28E-56SGTC_5394859-0462 2.5 μMChemDiv (Drug-like library)464994740.0222222RNA pol III & RNase P/MRP
0.2037.75E-56SGTC_2544sphondin 68.5 μMMicrosource (Natural product library)1081040.106667RNA pol III & RNase P/MRP
0.2018.92E-55SGTC_10164226-1401 38.0 μMChemDiv (Drug-like library)29016130.0879121RNA pol III & RNase P/MRP
0.1924.03E-50SGTC_32619137475 49.5 μMChembridge (Drug-like library)170276900.0689655RPP1 & pyrimidine depletion
0.1911.50E-49SGTC_2612dictamnine 15.5 μMMicrosource (Natural product library)680850.114286RNA pol III & RNase P/MRP
0.1713.79E-40SGTC_1642st009582 77.7 μMTimTec (Natural product derivative library)67393110.108108RPP1 & pyrimidine depletion
0.1681.62E-38SGTC_1930st074634 34.3 μMTimTec (Natural product derivative library)6842530.0657895RNA pol III & RNase P/MRP
0.1584.14E-34SGTC_2522biochanin a 4.9 μMMicrosource (Natural product library)52803730.146667RNA pol III & RNase P/MRP
0.1553.13E-33SGTC_2509methyl 7-deshydroxypyrogallin-4-carboxylate 54.6 μMMicrosource (Natural product library)50988840.169014RPP1 & pyrimidine depletion
0.1555.21E-33SGTC_28609025725 39.0 μMChembridge (Drug-like library)405462480.0697674RPP1 & pyrimidine depletion

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_265mycophenolic acid32.93 μM1446541Miscellaneous320.33713.15726RPP1 & pyrimidine depletion
SGTC_920mycophenolic acid780.42 nM1446541TimTec (Natural product library)320.33713.15726
SGTC_1123mangostin811.29 nM0.3382355281650TimTec (Natural product library)410.459645.93536mitochondrial response to ROS
SGTC_2594a-mangostin100 μM0.27142945934201Microsource (Natural product library)452.539386.61106
SGTC_2496diffractaic acid79.83 μM0.22857194870Microsource (Natural product library)374.384484.56127
SGTC_918glabranin770.73 nM0.220779124049TimTec (Natural product library)324.37044.47224
SGTC_2529gangaleoidin93.61 μM0.2179494565183Microsource (Natural product library)413.205564.8917
SGTC_2589lobaric acid100 μM0.20689773157Microsource (Natural product library)456.485025.99328
SGTC_2561acetosyringone100 μM0.2033917198Microsource (Natural product library)196.199881.29514
SGTC_1959st07709155.8 μM0.197183821356TimTec (Natural product derivative library)314.289462.61926RPP1 & pyrimidine depletion
SGTC_1593justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one54.9 μM0.194805442882TimTec (Pure natural product library)364.348143.60106redox potentiating