usnic acid

2,6-diacetyl-7,9-dihydroxy-8,9b-dimethyldibenzofuran-1,3-dione

An anti-infective, antifungal and antitrichomonal agent.

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 

PubChem MeSH terms: Anti-Infective Agents;Antifungal Agents;Antitrichomonal Agents



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2690
Screen concentration 100.0 μM
Source Miscellaneous
PubChem CID 5646
SMILES CC1=C(C(=C2C(=C1O)C3(C(=CC(=O)C(C3=O)C(=O)C)O2)C)C(=O)C)O
Standardized SMILES CC(=O)C1C(=O)C=C2Oc3c(C(=O)C)c(O)c(C)c(O)c3C2(C)C1=O
Molecular weight 344.3154
ALogP 1.08
H-bond donor count 2
H-bond acceptor count 7
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 0
% growth inhibition (Hom. pool) -1.09


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5646
Download HIP data (tab-delimited text)  (excel)
Gene:ALG2(YGL065C)|FD-Score:3.33|P-value:4.37E-4|Clearance:0.14||SGD DESC:Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol Gene:ARC40(YBR234C)|FD-Score:-3.45|P-value:2.78E-4|Clearance:0||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:CDC34(YDR054C)|FD-Score:-5.58|P-value:1.23E-8|Clearance:0||SGD DESC:Ubiquitin-conjugating enzyme (E2); catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation; protein abundance increases in response to DNA replication stress Gene:CDC8(YJR057W)|FD-Score:3.7|P-value:1.06E-4|Clearance:0.05||SGD DESC:Thymidylate and uridylate kinase, functions in de novo biosynthesis of pyrimidine deoxyribonucleotides; converts dTMP to dTDP and dUMP to dUTP; essential for mitotic and meiotic DNA replication; homologous to S. pombe Tmp1p Gene:DHR2(YKL078W)|FD-Score:3.19|P-value:7.20E-4|Clearance:0.21||SGD DESC:Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis Gene:ERD2(YBL040C)|FD-Score:4.48|P-value:3.82E-6|Clearance:0.33||SGD DESC:HDEL receptor, an integral membrane protein that binds to the HDEL motif in proteins destined for retention in the endoplasmic reticulum; has a role in maintenance of normal levels of ER-resident proteins Gene:GNA1(YFL017C)|FD-Score:-9.52|P-value:9.01E-22|Clearance:0||SGD DESC:Evolutionarily conserved glucosamine-6-phosphate acetyltransferase required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA Gene:KEI1(YDR367W)|FD-Score:3.34|P-value:4.15E-4|Clearance:0.01||SGD DESC:Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene Gene:LCP5(YER127W)|FD-Score:-4.09|P-value:2.14E-5|Clearance:0||SGD DESC:Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus Gene:MTR4(YJL050W)|FD-Score:7.11|P-value:5.76E-13|Clearance:2.09||SGD DESC:ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing Gene:NCB2(YDR397C)|FD-Score:3.87|P-value:5.45E-5|Clearance:0.16||SGD DESC:Subunit of a heterodimeric NC2 transcription regulator complex with Bur6p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2beta Gene:NOP56(YLR197W)|FD-Score:5.02|P-value:2.62E-7|Clearance:0.54||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:PRP38(YGR075C)|FD-Score:3.46|P-value:2.68E-4|Clearance:0.12||SGD DESC:Unique component of the U4/U6.U5 tri-snRNP particle, required for conformational changes which result in the catalytic activation of the spliceosome; dispensible for spliceosome assembly Gene:RAT1(YOR048C)|FD-Score:-3.67|P-value:1.20E-4|Clearance:0||SGD DESC:Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination Gene:RIM2(YBR192W)|FD-Score:3.98|P-value:3.39E-5|Clearance:0.11||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RLP24(YLR009W)|FD-Score:4.14|P-value:1.73E-5|Clearance:0.16||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:SDH3(YKL141W)|FD-Score:3.66|P-value:1.27E-4|Clearance:0.2||SGD DESC:Subunit of both succinate dehydrogenase and of TIM22 translocase; functions as the cytochrome b subunit of succinate dehydrogenase, which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex Gene:ALG2(YGL065C)|FD-Score:3.33|P-value:4.37E-4|Clearance:0.14||SGD DESC:Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol Gene:ARC40(YBR234C)|FD-Score:-3.45|P-value:2.78E-4|Clearance:0||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:CDC34(YDR054C)|FD-Score:-5.58|P-value:1.23E-8|Clearance:0||SGD DESC:Ubiquitin-conjugating enzyme (E2); catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation; protein abundance increases in response to DNA replication stress Gene:CDC8(YJR057W)|FD-Score:3.7|P-value:1.06E-4|Clearance:0.05||SGD DESC:Thymidylate and uridylate kinase, functions in de novo biosynthesis of pyrimidine deoxyribonucleotides; converts dTMP to dTDP and dUMP to dUTP; essential for mitotic and meiotic DNA replication; homologous to S. pombe Tmp1p Gene:DHR2(YKL078W)|FD-Score:3.19|P-value:7.20E-4|Clearance:0.21||SGD DESC:Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis Gene:ERD2(YBL040C)|FD-Score:4.48|P-value:3.82E-6|Clearance:0.33||SGD DESC:HDEL receptor, an integral membrane protein that binds to the HDEL motif in proteins destined for retention in the endoplasmic reticulum; has a role in maintenance of normal levels of ER-resident proteins Gene:GNA1(YFL017C)|FD-Score:-9.52|P-value:9.01E-22|Clearance:0||SGD DESC:Evolutionarily conserved glucosamine-6-phosphate acetyltransferase required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA Gene:KEI1(YDR367W)|FD-Score:3.34|P-value:4.15E-4|Clearance:0.01||SGD DESC:Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene Gene:LCP5(YER127W)|FD-Score:-4.09|P-value:2.14E-5|Clearance:0||SGD DESC:Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus Gene:MTR4(YJL050W)|FD-Score:7.11|P-value:5.76E-13|Clearance:2.09||SGD DESC:ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing Gene:NCB2(YDR397C)|FD-Score:3.87|P-value:5.45E-5|Clearance:0.16||SGD DESC:Subunit of a heterodimeric NC2 transcription regulator complex with Bur6p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2beta Gene:NOP56(YLR197W)|FD-Score:5.02|P-value:2.62E-7|Clearance:0.54||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:PRP38(YGR075C)|FD-Score:3.46|P-value:2.68E-4|Clearance:0.12||SGD DESC:Unique component of the U4/U6.U5 tri-snRNP particle, required for conformational changes which result in the catalytic activation of the spliceosome; dispensible for spliceosome assembly Gene:RAT1(YOR048C)|FD-Score:-3.67|P-value:1.20E-4|Clearance:0||SGD DESC:Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination Gene:RIM2(YBR192W)|FD-Score:3.98|P-value:3.39E-5|Clearance:0.11||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RLP24(YLR009W)|FD-Score:4.14|P-value:1.73E-5|Clearance:0.16||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:SDH3(YKL141W)|FD-Score:3.66|P-value:1.27E-4|Clearance:0.2||SGD DESC:Subunit of both succinate dehydrogenase and of TIM22 translocase; functions as the cytochrome b subunit of succinate dehydrogenase, which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5646
Download HOP data (tab-delimited text)  (excel)
Gene:AAD4(YDL243C)|FD-Score:4.33|P-value:7.38E-6||SGD DESC:Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin Gene:ACE2(YLR131C)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:AIM25(YJR100C)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Putative protein of unknown function; non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to murine NOR1; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:AIM37(YNL100W)|FD-Score:3.28|P-value:5.26E-4||SGD DESC:Mitochondrial inner membrane protein; component of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:ALD6(YPL061W)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:APE4(YHR113W)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Cytoplasmic aspartyl aminopeptidase that may also function in the vacuole; CVT pathway cargo protein; cleaves unblocked N-terminal acidic amino acids from peptide substrates; forms a 12-subunit homo-oligomer; M18 metalloprotease family Gene:ASN2(YGR124W)|FD-Score:-4.13|P-value:1.85E-5||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN2 has a paralog, ASN1, that arose from the whole genome duplication Gene:ATG7(YHR171W)|FD-Score:-3.72|P-value:9.97E-5||SGD DESC:Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation Gene:ATH1(YPR026W)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Acid trehalase required for utilization of extracellular trehalose Gene:ATR1(YML116W)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Multidrug efflux pump of the major facilitator superfamily; required for resistance to aminotriazole and 4-nitroquinoline-N-oxide; ATR1 has a paralog, YMR279C, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:BIK1(YCL029C)|FD-Score:6.76|P-value:6.84E-12||SGD DESC:Microtubule-associated protein, component of the interface between microtubules and kinetochore, involved in sister chromatid separation; essential in polyploid cells but not in haploid or diploid cells; ortholog of mammalian CLIP-170 Gene:BUD3(YCL014W)|FD-Score:3.78|P-value:7.92E-5||SGD DESC:Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding Gene:CBP1(YJL209W)|FD-Score:-4.23|P-value:1.18E-5||SGD DESC:Mitochondrial protein, regulator of COB mRNA stability and translation; interacts with the 5'-untranslated region of the COB mRNA; found in a complex at the inner membrane along with Pet309p; localizes to mitochondrial foci upon DNA replication stress Gene:CBR1(YIL043C)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia Gene:CHL4(YDR254W)|FD-Score:3.75|P-value:8.76E-5||SGD DESC:Outer kinetochore protein required for chromosome stability; involved in new kinetochore assembly and sister chromatid cohesion; peripheral component of the Ctf19 kinetochore complex that interacts with Ctf19p, Ctf3p, Iml3p and Mif2p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-N and fission yeast mis15 Gene:COX7(YMR256C)|FD-Score:-5.54|P-value:1.49E-8||SGD DESC:Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CYK3(YDL117W)|FD-Score:-3.72|P-value:9.83E-5||SGD DESC:SH3-domain protein located in the bud neck and cytokinetic actin ring; relocalizes from bud neck to nucleus upon DNA replication stress; activates the chitin synthase activity of Chs2p during cytokinesis; suppressor of growth and cytokinesis defects of chs2 phospho-mutants Gene:DCC1(YCL016C)|FD-Score:-3.19|P-value:7.04E-4||SGD DESC:Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance Gene:DGA1(YOR245C)|FD-Score:3.26|P-value:5.62E-4||SGD DESC:Diacylglycerol acyltransferase, catalyzes the terminal step of triacylglycerol (TAG) formation, acylates diacylglycerol using acyl-CoA as an acyl donor, localized to lipid particles Gene:DOA1(YKL213C)|FD-Score:3.97|P-value:3.58E-5||SGD DESC:WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress Gene:ECM2(YBR065C)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Pre-mRNA splicing factor, facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p Gene:ELF1(YKL160W)|FD-Score:3.13|P-value:8.66E-4||SGD DESC:Transcription elongation factor that contains a conserved zinc finger domain; implicated in the maintenance of proper chromatin structure in actively transcribed regions; deletion inhibits Brome mosaic virus (BMV) gene expression Gene:EXO5(YBR163W)|FD-Score:-3.35|P-value:4.01E-4||SGD DESC:Mitochondrial 5'-3' exonuclease and sliding exonuclease, required for mitochondrial genome maintenance; distantly related to the RecB nuclease domain of bacterial RecBCD recombinases; may be regulated by the transcription factor Ace2 Gene:FLR1(YBR008C)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; involved in efflux of fluconazole, diazaborine, benomyl, methotrexate, and other drugs; expression induced in cells treated with the mycotoxin patulin; relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:FMP45(YDL222C)|FD-Score:3.15|P-value:8.25E-4||SGD DESC:Integral membrane protein localized to mitochondria (untagged protein); required for sporulation and maintaining sphingolipid content; has sequence similarity to SUR7 and YNL194C Gene:FPR2(YDR519W)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:Membrane-bound peptidyl-prolyl cis-trans isomerase (PPIase); binds to the drugs FK506 and rapamycin; expression pattern suggests possible involvement in ER protein trafficking; relocalizes from nucleus to vacuole upon DNA replication stress Gene:GIT1(YCR098C)|FD-Score:3.22|P-value:6.39E-4||SGD DESC:Plasma membrane permease, mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability Gene:GLO1(YML004C)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Monomeric glyoxalase I, catalyzes the detoxification of methylglyoxal (a by-product of glycolysis) via condensation with glutathione to produce S-D-lactoylglutathione; expression regulated by methylglyoxal levels and osmotic stress Gene:GRX7(YBR014C)|FD-Score:4.51|P-value:3.21E-6||SGD DESC:Cis-golgi localized monothiol glutaredoxin; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; does not bind metal ions; GRX7 has a paralog, GRX6, that arose from the whole genome duplication Gene:HST2(YPL015C)|FD-Score:5.54|P-value:1.48E-8||SGD DESC:Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export Gene:HTZ1(YOL012C)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin Gene:IDS2(YJL146W)|FD-Score:3.86|P-value:5.61E-5||SGD DESC:Protein involved in modulation of Ime2p activity during meiosis, appears to act indirectly to promote Ime2p-mediated late meiotic functions; found in growing cells and degraded during sporulation Gene:IFM1(YOL023W)|FD-Score:-4.59|P-value:2.19E-6||SGD DESC:Mitochondrial translation initiation factor 2 Gene:IKS1(YJL057C)|FD-Score:4.64|P-value:1.77E-6||SGD DESC:Protein kinase of unknown cellular role; putative serine/threonine kinase; expression is induced during mild heat stress; deletion mutants are hypersensitive to copper sulphate and resistant to sorbate; interacts with an N-terminal fragment of Sst2p Gene:IRC13(YOR235W_d)|FD-Score:-3.97|P-value:3.55E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci Gene:KEL2(YGR238C)|FD-Score:3.13|P-value:8.71E-4||SGD DESC:Protein that functions in a complex with Kel1p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate Gene:LDH1(YBR204C)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Serine hydrolase; exhibits active esterase plus weak triacylglycerol lipase activities; proposed role in lipid homeostasis, regulating phospholipid and non-polar lipid levels and required for mobilization of LD-stored lipids; localizes to the lipid droplet (LD) surface; contains a classical serine containing catalytic triad (GxSxG motif) Gene:LIA1(YJR070C)|FD-Score:4.16|P-value:1.58E-5||SGD DESC:Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress Gene:LRE1(YCL051W)|FD-Score:5.47|P-value:2.28E-8||SGD DESC:Protein involved in control of cell wall structure and stress response; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway; <br>LRE1 has a paralog, HLR1, that arose from the whole genome duplication Gene:LSB6(YJL100W)|FD-Score:9.5|P-value:1.07E-21||SGD DESC:Type II phosphatidylinositol 4-kinase that binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization Gene:MDY2(YOL111C)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes Gene:MEP2(YNL142W)|FD-Score:-3.21|P-value:6.67E-4||SGD DESC:Ammonium permease involved in regulation of pseudohyphal growth; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation Gene:MLF3(YNL074C)|FD-Score:3.95|P-value:3.99E-5||SGD DESC:Serine-rich protein of unknown function, predicted to be palmitoylated; overproduction suppresses the growth inhibition caused by exposure to the immunosuppressant leflunomide Gene:MLP2(YIL149C)|FD-Score:-3.84|P-value:6.15E-5||SGD DESC:Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length Gene:MND2(YIR025W)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:MOS1(YCL057C-A)|FD-Score:3.3|P-value:4.79E-4||SGD DESC:Mitochondrial protein essential for proper inner membrane organization; conserved component of the mitochondrial inner membrane organizing system (MICOS, MINOS, or MitOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture; ortholog of human MINOS1 Gene:MRPL22(YNL177C)|FD-Score:-3.15|P-value:8.07E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL8(YJL063C)|FD-Score:-3.56|P-value:1.82E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSR1(YHR091C)|FD-Score:-4.16|P-value:1.61E-5||SGD DESC:Mitochondrial arginyl-tRNA synthetase; mutations in human ortholog are associated with pontocerebellar hypoplasia type 6 Gene:MUS81(YDR386W)|FD-Score:3.28|P-value:5.15E-4||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:PFK26(YIL107C)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:6-phosphofructo-2-kinase, inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate; has negligible fructose-2,6-bisphosphatase activity; transcriptional regulation involves protein kinase A Gene:PHR1(YOR386W)|FD-Score:-3.1|P-value:9.66E-4||SGD DESC:DNA photolyase involved in photoreactivation, repairs pyrimidine dimers in the presence of visible light; induced by DNA damage; regulated by transcriptional repressor Rph1p Gene:PMU1(YKL128C)|FD-Score:5.19|P-value:1.04E-7||SGD DESC:Putative phosphomutase, contains a region homologous to the active site of phosphomutases; overexpression suppresses the histidine auxotrophy of an ade3 ade16 ade17 triple mutant and the temperature sensitivity of a tps2 mutant Gene:PNS1(YOR161C)|FD-Score:-3.18|P-value:7.36E-4||SGD DESC:Protein of unknown function; has similarity to Torpedo californica tCTL1p, which is postulated to be a choline transporter, neither null mutation nor overexpression affects choline transport Gene:PTP3(YER075C)|FD-Score:3.88|P-value:5.31E-5||SGD DESC:Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the cytoplasm Gene:PUF6(YDR496C)|FD-Score:4.84|P-value:6.58E-7||SGD DESC:Pumilio-homology domain protein that binds the 3' UTR of ASH1 mRNA and represses its translation, resulting in proper asymmetric localization of ASH1 mRNA; also co-sediments with the 60S ribosomal subunit and is required for its biogenesis Gene:RAD1(YPL022W)|FD-Score:5.61|P-value:1.03E-8||SGD DESC:Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein Gene:RAD2(YGR258C)|FD-Score:4.65|P-value:1.65E-6||SGD DESC:Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein Gene:RPL12A(YEL054C)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Ribosomal 60S subunit protein L12A; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12A has a paralog, RPL12B, that arose from the whole genome duplication Gene:RPL34B(YIL052C)|FD-Score:-5.01|P-value:2.74E-7||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:RPS7A(YOR096W)|FD-Score:-3.22|P-value:6.46E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7A has a paralog, RPS7B, that arose from the whole genome duplication Gene:RTR2(YDR066C_p)|FD-Score:5.59|P-value:1.15E-8||SGD DESC:Protein of unknown function; exhibits genetic interactions with Rtr1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YDR066C is not an essential gene; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress; RTR2 has a paralog, RTR1, that arose from the whole genome duplication Gene:RTT102(YGR275W)|FD-Score:3.31|P-value:4.63E-4||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; suggested role in chromosome maintenance; possible weak regulator of Ty1 transposition; protein abundance increases in response to DNA replication stress Gene:SAP155(YFR040W)|FD-Score:3.25|P-value:5.71E-4||SGD DESC:Protein required for function of the Sit4p protein phosphatase; forms a complex with Sit4p; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p; protein abundance increases in response to DNA replication stress Gene:SAP4(YGL229C)|FD-Score:3.19|P-value:7.12E-4||SGD DESC:Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p Gene:SEG2(YKL105C_p)|FD-Score:4.52|P-value:3.11E-6||SGD DESC:Eisosome component and paralog of Seg1p; likely plays only minor role in eisosome assembly; shown to interact with Seg1p; similar to A. gossypii SEG gene which is important for stabilizing eisosomes Gene:SEY1(YOR165W)|FD-Score:-3.1|P-value:9.71E-4||SGD DESC:Dynamin-like GTPase that mediates homotypic ER fusion; has a role in ER morphology; interacts physically and genetically with Yop1p and Rtn1p; functional ortholog of the human atlastin ATL1, defects in which cause a form of the human disease hereditary spastic paraplegia; homolog of Arabidopsis RHD3 Gene:SKS1(YPL026C)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:Putative serine/threonine protein kinase; involved in the adaptation to low concentrations of glucose independent of the SNF3 regulated pathway Gene:SLS1(YLR139C)|FD-Score:-3.84|P-value:6.18E-5||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SNF5(YBR289W)|FD-Score:3.17|P-value:7.52E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions Gene:SOV1(YMR066W)|FD-Score:-3.38|P-value:3.60E-4||SGD DESC:Mitochondrial protein of unknown function Gene:THI3(YDL080C)|FD-Score:4.35|P-value:6.79E-6||SGD DESC:Probable alpha-ketoisocaproate decarboxylase, may have a role in catabolism of amino acids to long-chain and complex alcohols; required for expression of enzymes involved in thiamine biosynthesis Gene:THI74(YDR438W)|FD-Score:4.98|P-value:3.13E-7||SGD DESC:Mitochondrial transporter repressible by thiamine; THI74 has a paralog, YML018C, that arose from the whole genome duplication Gene:TIM8(YJR135W-A)|FD-Score:4.39|P-value:5.60E-6||SGD DESC:Mitochondrial intermembrane space protein, forms a complex with Tim13p that delivers a subset of hydrophobic proteins to the TIM22 complex for inner membrane insertion; homolog of human TIMM8A, implicated in Mohr-Tranebjaerg syndrome Gene:TMA64(YDR117C)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; in mammals the corresponding protein, eIF2D, has been shown to possess translation initiation factor activity Gene:TOD6(YBL054W)|FD-Score:5.99|P-value:1.04E-9||SGD DESC:PAC motif binding protein involved in rRNA and ribosome biogenesis; subunit of the RPD3L histone deacetylase complex; Myb-like HTH transcription factor; hypophosphorylated by rapamycin treatment in a Sch9p-dependent manner; TOD6 has a paralog, DOT6, that arose from the whole genome duplication Gene:TPO4(YOR273C)|FD-Score:3.15|P-value:8.03E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane Gene:TPS2(YDR074W)|FD-Score:-3.17|P-value:7.55E-4||SGD DESC:Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in synthesis of the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress Gene:TRS65(YGR166W)|FD-Score:-4.65|P-value:1.65E-6||SGD DESC:Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response Gene:YBL008W-A(YBL008W-A_p)|FD-Score:4.45|P-value:4.25E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YCL075W(YCL075W)|FD-Score:6.95|P-value:1.82E-12||SGD DESC:Pseudogene: encodes fragment of Ty Pol protein Gene:YCR087W(YCR087W_d)|FD-Score:-3.18|P-value:7.31E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YCR087C-A; YCR087W is not an essential gene Gene:YDL121C(YDL121C_p)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDL121C is not an essential protein Gene:YER158C(YER158C_p)|FD-Score:4.28|P-value:9.18E-6||SGD DESC:Protein of unknown function; potentially phosphorylated by Cdc28p; YER158C has a paralog, AFR1, that arose from the whole genome duplication Gene:YFL042C(YFL042C_p)|FD-Score:5|P-value:2.81E-7||SGD DESC:Putative protein of unknown function; YFL042C is not an essential gene Gene:YFR012W-A(YFR012W-A_p)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Putative protein of unknown function; identified by homology Gene:YGL132W(YGL132W_d)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SNT2/YGL131C Gene:YIL054W(YIL054W_p)|FD-Score:-3.14|P-value:8.55E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YIL089W(YIL089W)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Protein of unknown function found in the ER and vacuole lumen; overexpression of YIL089W affects endocytic protein trafficking Gene:YJL067W(YJL067W_d)|FD-Score:6.84|P-value:3.99E-12||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR087W(YJR087W_d)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified genes STE18 and ECM2 Gene:YLR001C(YLR001C_p)|FD-Score:3.78|P-value:7.88E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; predicted to be palmitoylated Gene:YLR211C(YLR211C_p)|FD-Score:4.17|P-value:1.52E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR211C is not an essential gene; ORF contains an intron Gene:YMR082C(YMR082C_d)|FD-Score:-3.2|P-value:6.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNG1(YOR064C)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3, has similarity to the human tumor suppressor ING1 Gene:YNL143C(YNL143C_p)|FD-Score:-3.45|P-value:2.77E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YTA6(YPL074W)|FD-Score:4.28|P-value:9.14E-6||SGD DESC:Putative ATPase of the CDC48/PAS1/SEC18 (AAA) family; localized to the cortex of mother cells but not to daughter cells; relocalizes from cytoplasm to plasma membrane foci upon DNA replication stress Gene:ZDS1(YMR273C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Protein with a role in regulating Swe1p-dependent polarized growth; involved in maintaining Cdc55p in the cytoplasm where it promotes mitotic entry; involved in mitotic exit through Cdc14p regulation; interacts with silencing proteins at telomeres; has a role in Bcy1p localization; implicated in mRNA nuclear export; ZDS1 has a paralog, ZDS2, that arose from the whole genome duplication Gene:AAD4(YDL243C)|FD-Score:4.33|P-value:7.38E-6||SGD DESC:Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin Gene:ACE2(YLR131C)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:AIM25(YJR100C)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Putative protein of unknown function; non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to murine NOR1; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:AIM37(YNL100W)|FD-Score:3.28|P-value:5.26E-4||SGD DESC:Mitochondrial inner membrane protein; component of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:ALD6(YPL061W)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:APE4(YHR113W)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Cytoplasmic aspartyl aminopeptidase that may also function in the vacuole; CVT pathway cargo protein; cleaves unblocked N-terminal acidic amino acids from peptide substrates; forms a 12-subunit homo-oligomer; M18 metalloprotease family Gene:ASN2(YGR124W)|FD-Score:-4.13|P-value:1.85E-5||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN2 has a paralog, ASN1, that arose from the whole genome duplication Gene:ATG7(YHR171W)|FD-Score:-3.72|P-value:9.97E-5||SGD DESC:Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation Gene:ATH1(YPR026W)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Acid trehalase required for utilization of extracellular trehalose Gene:ATR1(YML116W)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Multidrug efflux pump of the major facilitator superfamily; required for resistance to aminotriazole and 4-nitroquinoline-N-oxide; ATR1 has a paralog, YMR279C, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:BIK1(YCL029C)|FD-Score:6.76|P-value:6.84E-12||SGD DESC:Microtubule-associated protein, component of the interface between microtubules and kinetochore, involved in sister chromatid separation; essential in polyploid cells but not in haploid or diploid cells; ortholog of mammalian CLIP-170 Gene:BUD3(YCL014W)|FD-Score:3.78|P-value:7.92E-5||SGD DESC:Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding Gene:CBP1(YJL209W)|FD-Score:-4.23|P-value:1.18E-5||SGD DESC:Mitochondrial protein, regulator of COB mRNA stability and translation; interacts with the 5'-untranslated region of the COB mRNA; found in a complex at the inner membrane along with Pet309p; localizes to mitochondrial foci upon DNA replication stress Gene:CBR1(YIL043C)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia Gene:CHL4(YDR254W)|FD-Score:3.75|P-value:8.76E-5||SGD DESC:Outer kinetochore protein required for chromosome stability; involved in new kinetochore assembly and sister chromatid cohesion; peripheral component of the Ctf19 kinetochore complex that interacts with Ctf19p, Ctf3p, Iml3p and Mif2p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-N and fission yeast mis15 Gene:COX7(YMR256C)|FD-Score:-5.54|P-value:1.49E-8||SGD DESC:Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CYK3(YDL117W)|FD-Score:-3.72|P-value:9.83E-5||SGD DESC:SH3-domain protein located in the bud neck and cytokinetic actin ring; relocalizes from bud neck to nucleus upon DNA replication stress; activates the chitin synthase activity of Chs2p during cytokinesis; suppressor of growth and cytokinesis defects of chs2 phospho-mutants Gene:DCC1(YCL016C)|FD-Score:-3.19|P-value:7.04E-4||SGD DESC:Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance Gene:DGA1(YOR245C)|FD-Score:3.26|P-value:5.62E-4||SGD DESC:Diacylglycerol acyltransferase, catalyzes the terminal step of triacylglycerol (TAG) formation, acylates diacylglycerol using acyl-CoA as an acyl donor, localized to lipid particles Gene:DOA1(YKL213C)|FD-Score:3.97|P-value:3.58E-5||SGD DESC:WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress Gene:ECM2(YBR065C)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Pre-mRNA splicing factor, facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p Gene:ELF1(YKL160W)|FD-Score:3.13|P-value:8.66E-4||SGD DESC:Transcription elongation factor that contains a conserved zinc finger domain; implicated in the maintenance of proper chromatin structure in actively transcribed regions; deletion inhibits Brome mosaic virus (BMV) gene expression Gene:EXO5(YBR163W)|FD-Score:-3.35|P-value:4.01E-4||SGD DESC:Mitochondrial 5'-3' exonuclease and sliding exonuclease, required for mitochondrial genome maintenance; distantly related to the RecB nuclease domain of bacterial RecBCD recombinases; may be regulated by the transcription factor Ace2 Gene:FLR1(YBR008C)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; involved in efflux of fluconazole, diazaborine, benomyl, methotrexate, and other drugs; expression induced in cells treated with the mycotoxin patulin; relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:FMP45(YDL222C)|FD-Score:3.15|P-value:8.25E-4||SGD DESC:Integral membrane protein localized to mitochondria (untagged protein); required for sporulation and maintaining sphingolipid content; has sequence similarity to SUR7 and YNL194C Gene:FPR2(YDR519W)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:Membrane-bound peptidyl-prolyl cis-trans isomerase (PPIase); binds to the drugs FK506 and rapamycin; expression pattern suggests possible involvement in ER protein trafficking; relocalizes from nucleus to vacuole upon DNA replication stress Gene:GIT1(YCR098C)|FD-Score:3.22|P-value:6.39E-4||SGD DESC:Plasma membrane permease, mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability Gene:GLO1(YML004C)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Monomeric glyoxalase I, catalyzes the detoxification of methylglyoxal (a by-product of glycolysis) via condensation with glutathione to produce S-D-lactoylglutathione; expression regulated by methylglyoxal levels and osmotic stress Gene:GRX7(YBR014C)|FD-Score:4.51|P-value:3.21E-6||SGD DESC:Cis-golgi localized monothiol glutaredoxin; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; does not bind metal ions; GRX7 has a paralog, GRX6, that arose from the whole genome duplication Gene:HST2(YPL015C)|FD-Score:5.54|P-value:1.48E-8||SGD DESC:Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export Gene:HTZ1(YOL012C)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin Gene:IDS2(YJL146W)|FD-Score:3.86|P-value:5.61E-5||SGD DESC:Protein involved in modulation of Ime2p activity during meiosis, appears to act indirectly to promote Ime2p-mediated late meiotic functions; found in growing cells and degraded during sporulation Gene:IFM1(YOL023W)|FD-Score:-4.59|P-value:2.19E-6||SGD DESC:Mitochondrial translation initiation factor 2 Gene:IKS1(YJL057C)|FD-Score:4.64|P-value:1.77E-6||SGD DESC:Protein kinase of unknown cellular role; putative serine/threonine kinase; expression is induced during mild heat stress; deletion mutants are hypersensitive to copper sulphate and resistant to sorbate; interacts with an N-terminal fragment of Sst2p Gene:IRC13(YOR235W_d)|FD-Score:-3.97|P-value:3.55E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci Gene:KEL2(YGR238C)|FD-Score:3.13|P-value:8.71E-4||SGD DESC:Protein that functions in a complex with Kel1p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate Gene:LDH1(YBR204C)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Serine hydrolase; exhibits active esterase plus weak triacylglycerol lipase activities; proposed role in lipid homeostasis, regulating phospholipid and non-polar lipid levels and required for mobilization of LD-stored lipids; localizes to the lipid droplet (LD) surface; contains a classical serine containing catalytic triad (GxSxG motif) Gene:LIA1(YJR070C)|FD-Score:4.16|P-value:1.58E-5||SGD DESC:Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress Gene:LRE1(YCL051W)|FD-Score:5.47|P-value:2.28E-8||SGD DESC:Protein involved in control of cell wall structure and stress response; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway; <br>LRE1 has a paralog, HLR1, that arose from the whole genome duplication Gene:LSB6(YJL100W)|FD-Score:9.5|P-value:1.07E-21||SGD DESC:Type II phosphatidylinositol 4-kinase that binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization Gene:MDY2(YOL111C)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Protein with a role in insertion of tail-anchored proteins into the ER membrane; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes Gene:MEP2(YNL142W)|FD-Score:-3.21|P-value:6.67E-4||SGD DESC:Ammonium permease involved in regulation of pseudohyphal growth; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation Gene:MLF3(YNL074C)|FD-Score:3.95|P-value:3.99E-5||SGD DESC:Serine-rich protein of unknown function, predicted to be palmitoylated; overproduction suppresses the growth inhibition caused by exposure to the immunosuppressant leflunomide Gene:MLP2(YIL149C)|FD-Score:-3.84|P-value:6.15E-5||SGD DESC:Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length Gene:MND2(YIR025W)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:MOS1(YCL057C-A)|FD-Score:3.3|P-value:4.79E-4||SGD DESC:Mitochondrial protein essential for proper inner membrane organization; conserved component of the mitochondrial inner membrane organizing system (MICOS, MINOS, or MitOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture; ortholog of human MINOS1 Gene:MRPL22(YNL177C)|FD-Score:-3.15|P-value:8.07E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL8(YJL063C)|FD-Score:-3.56|P-value:1.82E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSR1(YHR091C)|FD-Score:-4.16|P-value:1.61E-5||SGD DESC:Mitochondrial arginyl-tRNA synthetase; mutations in human ortholog are associated with pontocerebellar hypoplasia type 6 Gene:MUS81(YDR386W)|FD-Score:3.28|P-value:5.15E-4||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:PFK26(YIL107C)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:6-phosphofructo-2-kinase, inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate; has negligible fructose-2,6-bisphosphatase activity; transcriptional regulation involves protein kinase A Gene:PHR1(YOR386W)|FD-Score:-3.1|P-value:9.66E-4||SGD DESC:DNA photolyase involved in photoreactivation, repairs pyrimidine dimers in the presence of visible light; induced by DNA damage; regulated by transcriptional repressor Rph1p Gene:PMU1(YKL128C)|FD-Score:5.19|P-value:1.04E-7||SGD DESC:Putative phosphomutase, contains a region homologous to the active site of phosphomutases; overexpression suppresses the histidine auxotrophy of an ade3 ade16 ade17 triple mutant and the temperature sensitivity of a tps2 mutant Gene:PNS1(YOR161C)|FD-Score:-3.18|P-value:7.36E-4||SGD DESC:Protein of unknown function; has similarity to Torpedo californica tCTL1p, which is postulated to be a choline transporter, neither null mutation nor overexpression affects choline transport Gene:PTP3(YER075C)|FD-Score:3.88|P-value:5.31E-5||SGD DESC:Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the cytoplasm Gene:PUF6(YDR496C)|FD-Score:4.84|P-value:6.58E-7||SGD DESC:Pumilio-homology domain protein that binds the 3' UTR of ASH1 mRNA and represses its translation, resulting in proper asymmetric localization of ASH1 mRNA; also co-sediments with the 60S ribosomal subunit and is required for its biogenesis Gene:RAD1(YPL022W)|FD-Score:5.61|P-value:1.03E-8||SGD DESC:Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein Gene:RAD2(YGR258C)|FD-Score:4.65|P-value:1.65E-6||SGD DESC:Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein Gene:RPL12A(YEL054C)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Ribosomal 60S subunit protein L12A; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12A has a paralog, RPL12B, that arose from the whole genome duplication Gene:RPL34B(YIL052C)|FD-Score:-5.01|P-value:2.74E-7||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:RPS7A(YOR096W)|FD-Score:-3.22|P-value:6.46E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7A has a paralog, RPS7B, that arose from the whole genome duplication Gene:RTR2(YDR066C_p)|FD-Score:5.59|P-value:1.15E-8||SGD DESC:Protein of unknown function; exhibits genetic interactions with Rtr1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YDR066C is not an essential gene; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress; RTR2 has a paralog, RTR1, that arose from the whole genome duplication Gene:RTT102(YGR275W)|FD-Score:3.31|P-value:4.63E-4||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; suggested role in chromosome maintenance; possible weak regulator of Ty1 transposition; protein abundance increases in response to DNA replication stress Gene:SAP155(YFR040W)|FD-Score:3.25|P-value:5.71E-4||SGD DESC:Protein required for function of the Sit4p protein phosphatase; forms a complex with Sit4p; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p; protein abundance increases in response to DNA replication stress Gene:SAP4(YGL229C)|FD-Score:3.19|P-value:7.12E-4||SGD DESC:Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p Gene:SEG2(YKL105C_p)|FD-Score:4.52|P-value:3.11E-6||SGD DESC:Eisosome component and paralog of Seg1p; likely plays only minor role in eisosome assembly; shown to interact with Seg1p; similar to A. gossypii SEG gene which is important for stabilizing eisosomes Gene:SEY1(YOR165W)|FD-Score:-3.1|P-value:9.71E-4||SGD DESC:Dynamin-like GTPase that mediates homotypic ER fusion; has a role in ER morphology; interacts physically and genetically with Yop1p and Rtn1p; functional ortholog of the human atlastin ATL1, defects in which cause a form of the human disease hereditary spastic paraplegia; homolog of Arabidopsis RHD3 Gene:SKS1(YPL026C)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:Putative serine/threonine protein kinase; involved in the adaptation to low concentrations of glucose independent of the SNF3 regulated pathway Gene:SLS1(YLR139C)|FD-Score:-3.84|P-value:6.18E-5||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SNF5(YBR289W)|FD-Score:3.17|P-value:7.52E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions Gene:SOV1(YMR066W)|FD-Score:-3.38|P-value:3.60E-4||SGD DESC:Mitochondrial protein of unknown function Gene:THI3(YDL080C)|FD-Score:4.35|P-value:6.79E-6||SGD DESC:Probable alpha-ketoisocaproate decarboxylase, may have a role in catabolism of amino acids to long-chain and complex alcohols; required for expression of enzymes involved in thiamine biosynthesis Gene:THI74(YDR438W)|FD-Score:4.98|P-value:3.13E-7||SGD DESC:Mitochondrial transporter repressible by thiamine; THI74 has a paralog, YML018C, that arose from the whole genome duplication Gene:TIM8(YJR135W-A)|FD-Score:4.39|P-value:5.60E-6||SGD DESC:Mitochondrial intermembrane space protein, forms a complex with Tim13p that delivers a subset of hydrophobic proteins to the TIM22 complex for inner membrane insertion; homolog of human TIMM8A, implicated in Mohr-Tranebjaerg syndrome Gene:TMA64(YDR117C)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; in mammals the corresponding protein, eIF2D, has been shown to possess translation initiation factor activity Gene:TOD6(YBL054W)|FD-Score:5.99|P-value:1.04E-9||SGD DESC:PAC motif binding protein involved in rRNA and ribosome biogenesis; subunit of the RPD3L histone deacetylase complex; Myb-like HTH transcription factor; hypophosphorylated by rapamycin treatment in a Sch9p-dependent manner; TOD6 has a paralog, DOT6, that arose from the whole genome duplication Gene:TPO4(YOR273C)|FD-Score:3.15|P-value:8.03E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane Gene:TPS2(YDR074W)|FD-Score:-3.17|P-value:7.55E-4||SGD DESC:Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in synthesis of the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress Gene:TRS65(YGR166W)|FD-Score:-4.65|P-value:1.65E-6||SGD DESC:Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response Gene:YBL008W-A(YBL008W-A_p)|FD-Score:4.45|P-value:4.25E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YCL075W(YCL075W)|FD-Score:6.95|P-value:1.82E-12||SGD DESC:Pseudogene: encodes fragment of Ty Pol protein Gene:YCR087W(YCR087W_d)|FD-Score:-3.18|P-value:7.31E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YCR087C-A; YCR087W is not an essential gene Gene:YDL121C(YDL121C_p)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDL121C is not an essential protein Gene:YER158C(YER158C_p)|FD-Score:4.28|P-value:9.18E-6||SGD DESC:Protein of unknown function; potentially phosphorylated by Cdc28p; YER158C has a paralog, AFR1, that arose from the whole genome duplication Gene:YFL042C(YFL042C_p)|FD-Score:5|P-value:2.81E-7||SGD DESC:Putative protein of unknown function; YFL042C is not an essential gene Gene:YFR012W-A(YFR012W-A_p)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Putative protein of unknown function; identified by homology Gene:YGL132W(YGL132W_d)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SNT2/YGL131C Gene:YIL054W(YIL054W_p)|FD-Score:-3.14|P-value:8.55E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YIL089W(YIL089W)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Protein of unknown function found in the ER and vacuole lumen; overexpression of YIL089W affects endocytic protein trafficking Gene:YJL067W(YJL067W_d)|FD-Score:6.84|P-value:3.99E-12||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR087W(YJR087W_d)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified genes STE18 and ECM2 Gene:YLR001C(YLR001C_p)|FD-Score:3.78|P-value:7.88E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; predicted to be palmitoylated Gene:YLR211C(YLR211C_p)|FD-Score:4.17|P-value:1.52E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR211C is not an essential gene; ORF contains an intron Gene:YMR082C(YMR082C_d)|FD-Score:-3.2|P-value:6.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNG1(YOR064C)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3, has similarity to the human tumor suppressor ING1 Gene:YNL143C(YNL143C_p)|FD-Score:-3.45|P-value:2.77E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YTA6(YPL074W)|FD-Score:4.28|P-value:9.14E-6||SGD DESC:Putative ATPase of the CDC48/PAS1/SEC18 (AAA) family; localized to the cortex of mother cells but not to daughter cells; relocalizes from cytoplasm to plasma membrane foci upon DNA replication stress Gene:ZDS1(YMR273C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Protein with a role in regulating Swe1p-dependent polarized growth; involved in maintaining Cdc55p in the cytoplasm where it promotes mitotic entry; involved in mitotic exit through Cdc14p regulation; interacts with silencing proteins at telomeres; has a role in Bcy1p localization; implicated in mRNA nuclear export; ZDS1 has a paralog, ZDS2, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YJL050W7.115.76E-132.09MTR4ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing
YLR197W5.022.62E-70.54NOP56Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects
YBL040C4.483.82E-60.34ERD2HDEL receptor, an integral membrane protein that binds to the HDEL motif in proteins destined for retention in the endoplasmic reticulum; has a role in maintenance of normal levels of ER-resident proteins
YLR009W4.141.73E-50.16RLP24Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp
YBR192W3.983.39E-50.11RIM2Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
YDR397C3.875.45E-50.17NCB2Subunit of a heterodimeric NC2 transcription regulator complex with Bur6p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2beta
YJR057W3.701.06E-40.05CDC8Thymidylate and uridylate kinase, functions in de novo biosynthesis of pyrimidine deoxyribonucleotides; converts dTMP to dTDP and dUMP to dUTP; essential for mitotic and meiotic DNA replication; homologous to S. pombe Tmp1p
YKL141W3.661.27E-40.20SDH3Subunit of both succinate dehydrogenase and of TIM22 translocase; functions as the cytochrome b subunit of succinate dehydrogenase, which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex
YGR075C3.462.68E-40.12PRP38Unique component of the U4/U6.U5 tri-snRNP particle, required for conformational changes which result in the catalytic activation of the spliceosome; dispensible for spliceosome assembly
YDR367W3.344.15E-40.01KEI1Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene
YGL065C3.334.37E-40.14ALG2Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol
YKL078W3.197.20E-40.21DHR2Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis
YFR051C2.970.001470.03RET2Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER
YNR054C2.950.001600.07ESF2Essential nucleolar protein involved in pre-18S rRNA processing; binds to RNA and stimulates ATPase activity of Dbp8; involved in assembly of the small subunit (SSU) processome
YOR203W_d2.880.001970.07YOR203W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps 5' end of essential DED1 gene required for translation initiation

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YJL100W9.501.07E-21LSB6Type II phosphatidylinositol 4-kinase that binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization
YCL075W6.951.82E-12YCL075WPseudogene: encodes fragment of Ty Pol protein
YJL067W_d6.843.99E-12YJL067W_dDubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
YCL029C6.766.84E-12BIK1Microtubule-associated protein, component of the interface between microtubules and kinetochore, involved in sister chromatid separation; essential in polyploid cells but not in haploid or diploid cells; ortholog of mammalian CLIP-170
YBL054W5.991.04E-9TOD6PAC motif binding protein involved in rRNA and ribosome biogenesis; subunit of the RPD3L histone deacetylase complex; Myb-like HTH transcription factor; hypophosphorylated by rapamycin treatment in a Sch9p-dependent manner; TOD6 has a paralog, DOT6, that arose from the whole genome duplication
YPL022W5.611.03E-8RAD1Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein
YDR066C_p5.591.15E-8RTR2_pProtein of unknown function; exhibits genetic interactions with Rtr1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YDR066C is not an essential gene; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress; RTR2 has a paralog, RTR1, that arose from the whole genome duplication
YPL015C5.541.48E-8HST2Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export
YCL051W5.472.28E-8LRE1Protein involved in control of cell wall structure and stress response; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway;
LRE1 has a paralog, HLR1, that arose from the whole genome duplication
YKL128C5.191.04E-7PMU1Putative phosphomutase, contains a region homologous to the active site of phosphomutases; overexpression suppresses the histidine auxotrophy of an ade3 ade16 ade17 triple mutant and the temperature sensitivity of a tps2 mutant
YFL042C_p5.002.81E-7YFL042C_pPutative protein of unknown function; YFL042C is not an essential gene
YDR438W4.983.13E-7THI74Mitochondrial transporter repressible by thiamine; THI74 has a paralog, YML018C, that arose from the whole genome duplication
YDR496C4.846.58E-7PUF6Pumilio-homology domain protein that binds the 3' UTR of ASH1 mRNA and represses its translation, resulting in proper asymmetric localization of ASH1 mRNA; also co-sediments with the 60S ribosomal subunit and is required for its biogenesis
YGR258C4.651.65E-6RAD2Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein
YJL057C4.641.77E-6IKS1Protein kinase of unknown cellular role; putative serine/threonine kinase; expression is induced during mild heat stress; deletion mutants are hypersensitive to copper sulphate and resistant to sorbate; interacts with an N-terminal fragment of Sst2p

GO enrichment analysis for SGTC_2690
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0938.98E-13SGTC_2674-Nitroquinoline-1-oxide (4-NQO) 44.4 nMMiscellaneous59550.0441176DNA damage response
0.0691.08E-7SGTC_2694st077685 81.9 μMTimTec (Natural product derivative library)28380960.075endomembrane recycling
0.0631.12E-6SGTC_2420aureobasidin a 50.0 nMMiscellaneous99634300.0666667
0.0571.33E-5SGTC_29899073649 71.4 μMChembridge (Drug-like library)213672020.0843373
0.0552.17E-5SGTC_11443486-0183 12.5 μMChemDiv (Drug-like library)67440690.0675676
0.0525.71E-5SGTC_2115tiliquinol 12.8 μMChembridge (Fragment library)712080.0952381
0.0491.54E-4SGTC_3823229-0966 23.2 μMChemDiv (Drug-like library)57223700.0506329
0.0472.90E-4SGTC_23759072683 200.0 μMChembridge (Fragment library)167980290.08mitochondrial processes
0.0473.48E-4SGTC_7150868-0130 184.0 μMChemDiv (Drug-like library)28762580.097561
0.0464.05E-4SGTC_24495812982 45.8 μMMiscellaneous7761850.0945946
0.0464.68E-4SGTC_33459148410 3.6 μMChembridge (Drug-like library)49035960.0574713
0.0448.18E-4SGTC_24425158906 48.5 μMMiscellaneous18938350.0641026
0.0430.00100SGTC_1513013-0144 2.3 μMChemDiv (Drug-like library)9065580.0617284iron homeostasis
0.0420.00123SGTC_2687chenodiol 100.0 μMMicrosource (Natural product library)57021810.0963855
0.0420.00129SGTC_13651672-3266 55.4 μMChemDiv (Drug-like library)64158730.0421053

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_259usnic acid108.93 μM15646Miscellaneous344.315441.08427
SGTC_2529gangaleoidin93.61 μM0.2133334565183Microsource (Natural product library)413.205564.8917
SGTC_21235259487200 μM0.196972173474Chembridge (Fragment library)223.268281.09114
SGTC_1570verbenone133 μM0.19642929025TimTec (Pure natural product library)150.217562.08201
SGTC_2561acetosyringone100 μM0.19642917198Microsource (Natural product library)196.199881.29514
SGTC_2496diffractaic acid79.83 μM0.18840694870Microsource (Natural product library)374.384484.56127
SGTC_13973532-019020.1 μM0.18755322280ChemDiv (Drug-like library)230.259163.57713
SGTC_25534-methylesculetin85.09 μM0.1864415319502Microsource (Natural product library)192.168121.86224
SGTC_1569carminic acid40.6 μM0.18518514749TimTec (Pure natural product library)492.3864-0.436913
SGTC_2490carminic acid95.39 μM0.18518514749TimTec (Pure natural product library)492.3864-0.436913
SGTC_1959st07709155.8 μM0.173913821356TimTec (Natural product derivative library)314.289462.61926RPP1 & pyrimidine depletion