st077426

N-(4-ethylphenyl)-2-[[9-(4-methylphenyl)-6-oxo-3H-purin-8-yl]sulfanyl]acetamide

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2692
Screen concentration 47.7 μM
Source TimTec (Natural product derivative library)
PubChem CID 25238571
SMILES CCC1=CC=C(C=C1)NC(=O)CSC2=NC3=C(N2C4=CC=C(C=C4)C)NC=NC3=O
Standardized SMILES CCc1ccc(NC(=O)CSc2nc3c(O)ncnc3n2c4ccc(C)cc4)cc1
Molecular weight 419.4994
ALogP 4.12
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.6
% growth inhibition (Hom. pool) 0


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 25238571
Download HIP data (tab-delimited text)  (excel)
Gene:COG2(YGR120C)|FD-Score:5.08|P-value:1.86E-7|Clearance:0.63||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:FMN1(YDR236C)|FD-Score:3.3|P-value:4.75E-4|Clearance:0.07||SGD DESC:Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane Gene:GPN2(YOR262W)|FD-Score:-3.59|P-value:1.65E-4|Clearance:0||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion Gene:HEM3(YDL205C)|FD-Score:3.61|P-value:1.54E-4|Clearance:0.09||SGD DESC:Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme Gene:MCM7(YBR202W)|FD-Score:3.32|P-value:4.52E-4|Clearance:0.01||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:MTW1(YAL034W-A)|FD-Score:3.52|P-value:2.15E-4|Clearance:0.07||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly Gene:NCP1(YHR042W)|FD-Score:3.45|P-value:2.84E-4|Clearance:0.13||SGD DESC:NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p Gene:NOP4(YPL043W)|FD-Score:3.13|P-value:8.70E-4|Clearance:0.05||SGD DESC:Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs) Gene:RNA1(YMR235C)|FD-Score:5.18|P-value:1.10E-7|Clearance:0.63||SGD DESC:GTPase activating protein (GAP) for Gsp1p, involved in nuclear transport Gene:RPN7(YPR108W)|FD-Score:-3.34|P-value:4.16E-4|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits Gene:RRP45(YDR280W)|FD-Score:4.49|P-value:3.53E-6|Clearance:0.63||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:SEC24(YIL109C)|FD-Score:3.24|P-value:6.00E-4|Clearance:0.11||SGD DESC:Component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb2p and Sfb3p Gene:SPC42(YKL042W)|FD-Score:-5.06|P-value:2.07E-7|Clearance:0||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane Gene:STU1(YBL034C)|FD-Score:5.45|P-value:2.55E-8|Clearance:0.63||SGD DESC:Component of the mitotic spindle that binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles Gene:TRS23(YDR246W)|FD-Score:3.86|P-value:5.71E-5|Clearance:0.03||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic; human homolog is TRAPPC4 Gene:UTP5(YDR398W)|FD-Score:4.61|P-value:1.98E-6|Clearance:0.63||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:YLR198C(YLR198C_d)|FD-Score:3.82|P-value:6.59E-5|Clearance:0.21||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W Gene:COG2(YGR120C)|FD-Score:5.08|P-value:1.86E-7|Clearance:0.63||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:FMN1(YDR236C)|FD-Score:3.3|P-value:4.75E-4|Clearance:0.07||SGD DESC:Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane Gene:GPN2(YOR262W)|FD-Score:-3.59|P-value:1.65E-4|Clearance:0||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion Gene:HEM3(YDL205C)|FD-Score:3.61|P-value:1.54E-4|Clearance:0.09||SGD DESC:Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme Gene:MCM7(YBR202W)|FD-Score:3.32|P-value:4.52E-4|Clearance:0.01||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:MTW1(YAL034W-A)|FD-Score:3.52|P-value:2.15E-4|Clearance:0.07||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly Gene:NCP1(YHR042W)|FD-Score:3.45|P-value:2.84E-4|Clearance:0.13||SGD DESC:NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p Gene:NOP4(YPL043W)|FD-Score:3.13|P-value:8.70E-4|Clearance:0.05||SGD DESC:Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs) Gene:RNA1(YMR235C)|FD-Score:5.18|P-value:1.10E-7|Clearance:0.63||SGD DESC:GTPase activating protein (GAP) for Gsp1p, involved in nuclear transport Gene:RPN7(YPR108W)|FD-Score:-3.34|P-value:4.16E-4|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits Gene:RRP45(YDR280W)|FD-Score:4.49|P-value:3.53E-6|Clearance:0.63||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:SEC24(YIL109C)|FD-Score:3.24|P-value:6.00E-4|Clearance:0.11||SGD DESC:Component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb2p and Sfb3p Gene:SPC42(YKL042W)|FD-Score:-5.06|P-value:2.07E-7|Clearance:0||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane Gene:STU1(YBL034C)|FD-Score:5.45|P-value:2.55E-8|Clearance:0.63||SGD DESC:Component of the mitotic spindle that binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles Gene:TRS23(YDR246W)|FD-Score:3.86|P-value:5.71E-5|Clearance:0.03||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic; human homolog is TRAPPC4 Gene:UTP5(YDR398W)|FD-Score:4.61|P-value:1.98E-6|Clearance:0.63||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:YLR198C(YLR198C_d)|FD-Score:3.82|P-value:6.59E-5|Clearance:0.21||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 25238571
Download HOP data (tab-delimited text)  (excel)
Gene:ACB1(YGR037C)|FD-Score:3.73|P-value:9.51E-5||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ASI2(YNL159C)|FD-Score:4.63|P-value:1.86E-6||SGD DESC:Integral inner nuclear membrane protein that acts with Asi1p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals Gene:ATP23(YNR020C)|FD-Score:-5.26|P-value:7.33E-8||SGD DESC:Putative metalloprotease of the mitochondrial inner membrane, required for processing of Atp6p; has an additional role in assembly of the F0 sector of the F1F0 ATP synthase complex; substrate of the Mia40p-Erv1p disulfide relay system, and folding is assisted by Mia40p Gene:ATR1(YML116W)|FD-Score:4.43|P-value:4.61E-6||SGD DESC:Multidrug efflux pump of the major facilitator superfamily; required for resistance to aminotriazole and 4-nitroquinoline-N-oxide; ATR1 has a paralog, YMR279C, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:BAS1(YKR099W)|FD-Score:3.4|P-value:3.36E-4||SGD DESC:Myb-related transcription factor involved in regulating basal and induced expression of genes of the purine and histidine biosynthesis pathways; also involved in regulation of meiotic recombination at specific genes Gene:BCH2(YKR027W)|FD-Score:-3.49|P-value:2.46E-4||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BCH2 has a paralog, CHS6, that arose from the whole genome duplication Gene:BDH1(YAL060W)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:NAD-dependent (R,R)-butanediol dehydrogenase, catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source Gene:CCH1(YGR217W)|FD-Score:-3.26|P-value:5.49E-4||SGD DESC:Voltage-gated high-affinity calcium channel involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and co-localizes with Mid1p, suggesting Cch1p and Mid1p function together Gene:CIT3(YPR001W)|FD-Score:4.69|P-value:1.39E-6||SGD DESC:Dual specificity mitochondrial citrate and methylcitrate synthase; catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and that of propionyl-CoA and oxaloacetate to form 2-methylcitrate Gene:CLD1(YGR110W)|FD-Score:-3.16|P-value:7.83E-4||SGD DESC:Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58 Gene:CNA1(YLR433C)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:Calcineurin A; one isoform (the other is CMP2) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1 Gene:COX13(YGL191W)|FD-Score:3.39|P-value:3.43E-4||SGD DESC:Subunit VIa of cytochrome c oxidase; present in a subclass of cytochrome c oxidase complexes that may have a role in mimimizing generation of reactive oxygen species; not essential for cytochrome c oxidase activity but may modulate activity in response to ATP; required for assembly of Rcf2p into cytochrome c oxidase - cytochrome bc1 supercomplexes Gene:COX15(YER141W)|FD-Score:4.13|P-value:1.80E-5||SGD DESC:Protein required for the hydroxylation of heme O to form heme A, which is an essential prosthetic group for cytochrome c oxidase Gene:COX7(YMR256C)|FD-Score:7.79|P-value:3.44E-15||SGD DESC:Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CWH43(YCR017C)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion Gene:DAP1(YPL170W)|FD-Score:3.28|P-value:5.28E-4||SGD DESC:Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis Gene:DMA1(YHR115C)|FD-Score:4.55|P-value:2.71E-6||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma2p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8 protein, similar to human Chfr; contains FHA and RING finger Gene:DOT1(YDR440W)|FD-Score:-3.76|P-value:8.45E-5||SGD DESC:Nucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response Gene:ECM15(YBL001C)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Non-essential protein of unknown function, likely exists as tetramer, may be regulated by the binding of small-molecule ligands (possibly sulfate ions), may have a role in yeast cell-wall biogenesis Gene:ECM7(YLR443W)|FD-Score:-3.14|P-value:8.30E-4||SGD DESC:Non-essential putative integral membrane protein with a role in calcium uptake; mutant has cell wall defects and Ca+ uptake deficiencies; transcription is induced under conditions of zinc deficiency Gene:FAR11(YNL127W)|FD-Score:4.93|P-value:4.14E-7||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:GDS1(YOR355W)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:Protein of unknown function, required for growth on glycerol as a carbon source; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GEP5(YLR091W)|FD-Score:-4.19|P-value:1.37E-5||SGD DESC:Protein of unknown function, required for mitochondrial genome maintenance; detected in highly purified mitochondria in high-throughput studies; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine Gene:GRX7(YBR014C)|FD-Score:3.13|P-value:8.74E-4||SGD DESC:Cis-golgi localized monothiol glutaredoxin; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; does not bind metal ions; GRX7 has a paralog, GRX6, that arose from the whole genome duplication Gene:GSY2(YLR258W)|FD-Score:-3.48|P-value:2.47E-4||SGD DESC:Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:HSL7(YBR133C)|FD-Score:8.64|P-value:2.81E-18||SGD DESC:Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress Gene:HTA1(YDR225W)|FD-Score:6.18|P-value:3.19E-10||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:IMG1(YCR046C)|FD-Score:3.23|P-value:6.27E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, required for respiration and for maintenance of the mitochondrial genome Gene:IRC22(YEL001C)|FD-Score:3.17|P-value:7.60E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; YEL001C is non-essential; null mutant displays increased levels of spontaneous Rad52p foci Gene:LST4(YKL176C)|FD-Score:-3.31|P-value:4.59E-4||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MOS1(YCL057C-A)|FD-Score:3.38|P-value:3.68E-4||SGD DESC:Mitochondrial protein essential for proper inner membrane organization; conserved component of the mitochondrial inner membrane organizing system (MICOS, MINOS, or MitOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture; ortholog of human MINOS1 Gene:MRS3(YJL133W)|FD-Score:5.2|P-value:1.02E-7||SGD DESC:Iron transporter, mediates Fe2+ transport across inner mito membrane; mitochondrial carrier family member; active under low-iron conditions; may transport other cations; MRS3 has a paralog, MRS4, that arose from the whole genome duplication Gene:MTC5(YDR128W)|FD-Score:4.21|P-value:1.30E-5||SGD DESC:Subunit of the SEA (Seh1-associated) complex; SEA is a coatomer-related complex that associates dynamically with the vacuole; has N-terminal WD-40 repeats and a C-terminal RING motif; mtc5 is synthetically sick with cdc13-1; relative distribution to vacuolar membrane punctae decreases upon DNA replication stress Gene:NAM7(YMR080C)|FD-Score:-3.34|P-value:4.18E-4||SGD DESC:ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance; forms cytoplasmic foci upon DNA replication stress Gene:NUC1(YJL208C)|FD-Score:4.13|P-value:1.79E-5||SGD DESC:Major mitochondrial nuclease, has RNAse and DNA endo- and exonucleolytic activities; has roles in mitochondrial recombination, apoptosis and maintenance of polyploidy Gene:NUP170(YBL079W)|FD-Score:4.07|P-value:2.31E-5||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication Gene:OPI9(YLR338W_d)|FD-Score:3.12|P-value:9.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:PAU23(YLR037C)|FD-Score:3.36|P-value:3.92E-4||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:PKH3(YDR466W)|FD-Score:-3.8|P-value:7.17E-5||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:PLB3(YOL011W)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro Gene:PMT7(YDR307W_p)|FD-Score:3.28|P-value:5.16E-4||SGD DESC:Putative protein mannosyltransferase similar to Pmt1p; has a potential role in protein O-glycosylation Gene:PPT1(YGR123C)|FD-Score:3.98|P-value:3.42E-5||SGD DESC:Protein serine/threonine phosphatase, regulates Hsp90 chaperone by affecting its ATPase and cochaperone binding activities; has similarity to human phosphatase PP5; present in both the nucleus and cytoplasm; expressed during logarithmic growth Gene:RAD18(YCR066W)|FD-Score:3.11|P-value:9.22E-4||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:REC114(YMR133W)|FD-Score:4.7|P-value:1.33E-6||SGD DESC:Protein involved in early stages of meiotic recombination; possibly involved in the coordination of recombination and meiotic division; mutations lead to premature initiation of the first meiotic division Gene:ROG1(YGL144C)|FD-Score:-3.61|P-value:1.50E-4||SGD DESC:Protein with putative serine active lipase domain; ROG1 has a paralog, YDL109C, that arose from the whole genome duplication Gene:RPL31A(YDL075W)|FD-Score:-4.19|P-value:1.39E-5||SGD DESC:Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication Gene:RPL40B(YKR094C)|FD-Score:-3.77|P-value:8.26E-5||SGD DESC:Ubiquitin-ribosomal 60S subunit protein L40B fusion protein; cleaved to yield ubiquitin and ribosomal protein L40B; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40B has a paralog, RPL40A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:3.31|P-value:4.64E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS25A(YGR027C)|FD-Score:-3.58|P-value:1.75E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S25, no bacterial homolog; RPS25A has a paralog, RPS25B, that arose from the whole genome duplication Gene:SAS5(YOR213C)|FD-Score:3.3|P-value:4.87E-4||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity Gene:SBE22(YHR103W)|FD-Score:3.29|P-value:4.92E-4||SGD DESC:Protein involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; involved in bud growth Gene:SEF1(YBL066C)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Putative transcription factor, has homolog in Kluyveromyces lactis Gene:SIZ1(YDR409W)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring Gene:SNO4(YMR322C_p)|FD-Score:5.95|P-value:1.33E-9||SGD DESC:Possible chaperone and cysteine protease, similar to bacterial Hsp31 and yeast Hsp31p, Hsp32p, and Hsp33p; DJ-1/ThiJ/PfpI superfamily member; predicted involvement in pyridoxine metabolism; induced by mild heat stress and copper deprivation Gene:SPI1(YER150W)|FD-Score:3.87|P-value:5.49E-5||SGD DESC:GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; similar to Sed1p Gene:SPO16(YHR153C)|FD-Score:3.12|P-value:9.02E-4||SGD DESC:Meiosis-specific protein involved in synaptonemal complex assembly; implicated in regulation of crossover formation; required for sporulation Gene:SRY1(YKL218C)|FD-Score:-4.08|P-value:2.27E-5||SGD DESC:3-hydroxyaspartate dehydratase, deaminates L-threo-3-hydroxyaspartate to form oxaloacetate and ammonia; required in the presence of hydroxyaspartate; highly similar to mouse serine racemase (Srr) but has no serine racemase activity Gene:STI1(YOR027W)|FD-Score:3.74|P-value:9.22E-5||SGD DESC:Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop Gene:TPO3(YPR156C)|FD-Score:6.07|P-value:6.40E-10||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; TPO3 has a paralog, TPO2, that arose from the whole genome duplication Gene:UIP4(YPL186C)|FD-Score:-6.04|P-value:7.69E-10||SGD DESC:Protein that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates; detected in a phosphorylated state in the mitochondrial outer membrane; also detected in ER and nuclear envelope Gene:XBP1(YIL101C)|FD-Score:3.22|P-value:6.37E-4||SGD DESC:Transcriptional repressor; binds to promoter sequences of the cyclin genes, CYS3, and SMF2; expression is induced by stress or starvation during mitosis, and late in meiosis; member of the Swi4p/Mbp1p family; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:YBR196C-A(YBR196C-A_p)|FD-Score:3.1|P-value:9.75E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR061W(YDR061W_p)|FD-Score:-4.12|P-value:1.87E-5||SGD DESC:Protein with similarity to ATP-binding cassette (ABC) transporter family members; lacks predicted membrane-spanning regions; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance Gene:YDR169C-A(YDR169C-A_p)|FD-Score:4.69|P-value:1.39E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YER039C-A(YER039C-A_p)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:Putative protein of unknown function; YER039C-A is not an essential gene Gene:YER097W(YER097W_d)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER121W(YER121W_p)|FD-Score:5.67|P-value:7.13E-9||SGD DESC:Putative protein of unknown function; may be involved in phosphatase regulation and/or generation of precursor metabolites and energy Gene:YGR125W(YGR125W_p)|FD-Score:-4.16|P-value:1.62E-5||SGD DESC:Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YIR044C(YIR044C)|FD-Score:4.61|P-value:2.05E-6||SGD DESC:Possible pseudogene in strain S288C; YIR044C and the adjacent ORF, YIR043C, together may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family Gene:YLR202C(YLR202C_d)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron Gene:YMR099C(YMR099C)|FD-Score:-3.6|P-value:1.61E-4||SGD DESC:Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS Gene:YMR253C(YMR253C_p)|FD-Score:4.45|P-value:4.26E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene Gene:YNL200C(YNL200C)|FD-Score:3.28|P-value:5.27E-4||SGD DESC:NADHX epimerase; catalyzes isomerization of (R)- and (S)-NADHX; homologous to AIBP in mammals and the N- terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YNL295W(YNL295W_p)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Putative protein of unknown function Gene:YNR029C(YNR029C_p)|FD-Score:4.37|P-value:6.22E-6||SGD DESC:Putative protein of unknown function, deletion confers reduced fitness in saline Gene:YNR061C(YNR061C_p)|FD-Score:3.17|P-value:7.72E-4||SGD DESC:Protein of unknown function; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:YOL160W(YOL160W_d)|FD-Score:3.24|P-value:5.89E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR020W-A(YOR020W-A_p)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Putative protein of unknown function, conserved in A. gossypii; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOR300W(YOR300W_d)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps with verified gene BUD7/YOR299W; mutation affects bipolar budding and bud site selection, though phenotype could be due to the mutation's effects on BUD7 Gene:YOS9(YDR057W)|FD-Score:-3.12|P-value:8.95E-4||SGD DESC:ER quality-control lectin; integral subunit of the HRD ligase; binds to glycans with terminal alpha-1,6 linked mannose on misfolded N-glycosylated proteins and participates in targeting proteins to ERAD; member of the OS-9 protein family Gene:YOX1(YML027W)|FD-Score:4.47|P-value:3.86E-6||SGD DESC:Homeobox transcriptional repressor; binds to Mcm1p and to early cell cycle boxes (ECBs) in the promoters of cell cycle-regulated genes expressed in M/G1 phase; expression is cell cycle-regulated; potential Cdc28p substrate; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOX1 has a paralog, YHP1, that arose from the whole genome duplication Gene:YPR077C(YPR077C_d)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression increased by deletion of NAP1 Gene:ACB1(YGR037C)|FD-Score:3.73|P-value:9.51E-5||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ASI2(YNL159C)|FD-Score:4.63|P-value:1.86E-6||SGD DESC:Integral inner nuclear membrane protein that acts with Asi1p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals Gene:ATP23(YNR020C)|FD-Score:-5.26|P-value:7.33E-8||SGD DESC:Putative metalloprotease of the mitochondrial inner membrane, required for processing of Atp6p; has an additional role in assembly of the F0 sector of the F1F0 ATP synthase complex; substrate of the Mia40p-Erv1p disulfide relay system, and folding is assisted by Mia40p Gene:ATR1(YML116W)|FD-Score:4.43|P-value:4.61E-6||SGD DESC:Multidrug efflux pump of the major facilitator superfamily; required for resistance to aminotriazole and 4-nitroquinoline-N-oxide; ATR1 has a paralog, YMR279C, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:BAS1(YKR099W)|FD-Score:3.4|P-value:3.36E-4||SGD DESC:Myb-related transcription factor involved in regulating basal and induced expression of genes of the purine and histidine biosynthesis pathways; also involved in regulation of meiotic recombination at specific genes Gene:BCH2(YKR027W)|FD-Score:-3.49|P-value:2.46E-4||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BCH2 has a paralog, CHS6, that arose from the whole genome duplication Gene:BDH1(YAL060W)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:NAD-dependent (R,R)-butanediol dehydrogenase, catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source Gene:CCH1(YGR217W)|FD-Score:-3.26|P-value:5.49E-4||SGD DESC:Voltage-gated high-affinity calcium channel involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and co-localizes with Mid1p, suggesting Cch1p and Mid1p function together Gene:CIT3(YPR001W)|FD-Score:4.69|P-value:1.39E-6||SGD DESC:Dual specificity mitochondrial citrate and methylcitrate synthase; catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and that of propionyl-CoA and oxaloacetate to form 2-methylcitrate Gene:CLD1(YGR110W)|FD-Score:-3.16|P-value:7.83E-4||SGD DESC:Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58 Gene:CNA1(YLR433C)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:Calcineurin A; one isoform (the other is CMP2) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1 Gene:COX13(YGL191W)|FD-Score:3.39|P-value:3.43E-4||SGD DESC:Subunit VIa of cytochrome c oxidase; present in a subclass of cytochrome c oxidase complexes that may have a role in mimimizing generation of reactive oxygen species; not essential for cytochrome c oxidase activity but may modulate activity in response to ATP; required for assembly of Rcf2p into cytochrome c oxidase - cytochrome bc1 supercomplexes Gene:COX15(YER141W)|FD-Score:4.13|P-value:1.80E-5||SGD DESC:Protein required for the hydroxylation of heme O to form heme A, which is an essential prosthetic group for cytochrome c oxidase Gene:COX7(YMR256C)|FD-Score:7.79|P-value:3.44E-15||SGD DESC:Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CWH43(YCR017C)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion Gene:DAP1(YPL170W)|FD-Score:3.28|P-value:5.28E-4||SGD DESC:Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis Gene:DMA1(YHR115C)|FD-Score:4.55|P-value:2.71E-6||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma2p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8 protein, similar to human Chfr; contains FHA and RING finger Gene:DOT1(YDR440W)|FD-Score:-3.76|P-value:8.45E-5||SGD DESC:Nucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response Gene:ECM15(YBL001C)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Non-essential protein of unknown function, likely exists as tetramer, may be regulated by the binding of small-molecule ligands (possibly sulfate ions), may have a role in yeast cell-wall biogenesis Gene:ECM7(YLR443W)|FD-Score:-3.14|P-value:8.30E-4||SGD DESC:Non-essential putative integral membrane protein with a role in calcium uptake; mutant has cell wall defects and Ca+ uptake deficiencies; transcription is induced under conditions of zinc deficiency Gene:FAR11(YNL127W)|FD-Score:4.93|P-value:4.14E-7||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:GDS1(YOR355W)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:Protein of unknown function, required for growth on glycerol as a carbon source; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GEP5(YLR091W)|FD-Score:-4.19|P-value:1.37E-5||SGD DESC:Protein of unknown function, required for mitochondrial genome maintenance; detected in highly purified mitochondria in high-throughput studies; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine Gene:GRX7(YBR014C)|FD-Score:3.13|P-value:8.74E-4||SGD DESC:Cis-golgi localized monothiol glutaredoxin; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; does not bind metal ions; GRX7 has a paralog, GRX6, that arose from the whole genome duplication Gene:GSY2(YLR258W)|FD-Score:-3.48|P-value:2.47E-4||SGD DESC:Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:HSL7(YBR133C)|FD-Score:8.64|P-value:2.81E-18||SGD DESC:Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress Gene:HTA1(YDR225W)|FD-Score:6.18|P-value:3.19E-10||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:IMG1(YCR046C)|FD-Score:3.23|P-value:6.27E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, required for respiration and for maintenance of the mitochondrial genome Gene:IRC22(YEL001C)|FD-Score:3.17|P-value:7.60E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; YEL001C is non-essential; null mutant displays increased levels of spontaneous Rad52p foci Gene:LST4(YKL176C)|FD-Score:-3.31|P-value:4.59E-4||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MOS1(YCL057C-A)|FD-Score:3.38|P-value:3.68E-4||SGD DESC:Mitochondrial protein essential for proper inner membrane organization; conserved component of the mitochondrial inner membrane organizing system (MICOS, MINOS, or MitOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture; ortholog of human MINOS1 Gene:MRS3(YJL133W)|FD-Score:5.2|P-value:1.02E-7||SGD DESC:Iron transporter, mediates Fe2+ transport across inner mito membrane; mitochondrial carrier family member; active under low-iron conditions; may transport other cations; MRS3 has a paralog, MRS4, that arose from the whole genome duplication Gene:MTC5(YDR128W)|FD-Score:4.21|P-value:1.30E-5||SGD DESC:Subunit of the SEA (Seh1-associated) complex; SEA is a coatomer-related complex that associates dynamically with the vacuole; has N-terminal WD-40 repeats and a C-terminal RING motif; mtc5 is synthetically sick with cdc13-1; relative distribution to vacuolar membrane punctae decreases upon DNA replication stress Gene:NAM7(YMR080C)|FD-Score:-3.34|P-value:4.18E-4||SGD DESC:ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance; forms cytoplasmic foci upon DNA replication stress Gene:NUC1(YJL208C)|FD-Score:4.13|P-value:1.79E-5||SGD DESC:Major mitochondrial nuclease, has RNAse and DNA endo- and exonucleolytic activities; has roles in mitochondrial recombination, apoptosis and maintenance of polyploidy Gene:NUP170(YBL079W)|FD-Score:4.07|P-value:2.31E-5||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication Gene:OPI9(YLR338W_d)|FD-Score:3.12|P-value:9.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:PAU23(YLR037C)|FD-Score:3.36|P-value:3.92E-4||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:PKH3(YDR466W)|FD-Score:-3.8|P-value:7.17E-5||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:PLB3(YOL011W)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro Gene:PMT7(YDR307W_p)|FD-Score:3.28|P-value:5.16E-4||SGD DESC:Putative protein mannosyltransferase similar to Pmt1p; has a potential role in protein O-glycosylation Gene:PPT1(YGR123C)|FD-Score:3.98|P-value:3.42E-5||SGD DESC:Protein serine/threonine phosphatase, regulates Hsp90 chaperone by affecting its ATPase and cochaperone binding activities; has similarity to human phosphatase PP5; present in both the nucleus and cytoplasm; expressed during logarithmic growth Gene:RAD18(YCR066W)|FD-Score:3.11|P-value:9.22E-4||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:REC114(YMR133W)|FD-Score:4.7|P-value:1.33E-6||SGD DESC:Protein involved in early stages of meiotic recombination; possibly involved in the coordination of recombination and meiotic division; mutations lead to premature initiation of the first meiotic division Gene:ROG1(YGL144C)|FD-Score:-3.61|P-value:1.50E-4||SGD DESC:Protein with putative serine active lipase domain; ROG1 has a paralog, YDL109C, that arose from the whole genome duplication Gene:RPL31A(YDL075W)|FD-Score:-4.19|P-value:1.39E-5||SGD DESC:Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication Gene:RPL40B(YKR094C)|FD-Score:-3.77|P-value:8.26E-5||SGD DESC:Ubiquitin-ribosomal 60S subunit protein L40B fusion protein; cleaved to yield ubiquitin and ribosomal protein L40B; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40B has a paralog, RPL40A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:3.31|P-value:4.64E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS25A(YGR027C)|FD-Score:-3.58|P-value:1.75E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S25, no bacterial homolog; RPS25A has a paralog, RPS25B, that arose from the whole genome duplication Gene:SAS5(YOR213C)|FD-Score:3.3|P-value:4.87E-4||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity Gene:SBE22(YHR103W)|FD-Score:3.29|P-value:4.92E-4||SGD DESC:Protein involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; involved in bud growth Gene:SEF1(YBL066C)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Putative transcription factor, has homolog in Kluyveromyces lactis Gene:SIZ1(YDR409W)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring Gene:SNO4(YMR322C_p)|FD-Score:5.95|P-value:1.33E-9||SGD DESC:Possible chaperone and cysteine protease, similar to bacterial Hsp31 and yeast Hsp31p, Hsp32p, and Hsp33p; DJ-1/ThiJ/PfpI superfamily member; predicted involvement in pyridoxine metabolism; induced by mild heat stress and copper deprivation Gene:SPI1(YER150W)|FD-Score:3.87|P-value:5.49E-5||SGD DESC:GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; similar to Sed1p Gene:SPO16(YHR153C)|FD-Score:3.12|P-value:9.02E-4||SGD DESC:Meiosis-specific protein involved in synaptonemal complex assembly; implicated in regulation of crossover formation; required for sporulation Gene:SRY1(YKL218C)|FD-Score:-4.08|P-value:2.27E-5||SGD DESC:3-hydroxyaspartate dehydratase, deaminates L-threo-3-hydroxyaspartate to form oxaloacetate and ammonia; required in the presence of hydroxyaspartate; highly similar to mouse serine racemase (Srr) but has no serine racemase activity Gene:STI1(YOR027W)|FD-Score:3.74|P-value:9.22E-5||SGD DESC:Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop Gene:TPO3(YPR156C)|FD-Score:6.07|P-value:6.40E-10||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; TPO3 has a paralog, TPO2, that arose from the whole genome duplication Gene:UIP4(YPL186C)|FD-Score:-6.04|P-value:7.69E-10||SGD DESC:Protein that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates; detected in a phosphorylated state in the mitochondrial outer membrane; also detected in ER and nuclear envelope Gene:XBP1(YIL101C)|FD-Score:3.22|P-value:6.37E-4||SGD DESC:Transcriptional repressor; binds to promoter sequences of the cyclin genes, CYS3, and SMF2; expression is induced by stress or starvation during mitosis, and late in meiosis; member of the Swi4p/Mbp1p family; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:YBR196C-A(YBR196C-A_p)|FD-Score:3.1|P-value:9.75E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR061W(YDR061W_p)|FD-Score:-4.12|P-value:1.87E-5||SGD DESC:Protein with similarity to ATP-binding cassette (ABC) transporter family members; lacks predicted membrane-spanning regions; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance Gene:YDR169C-A(YDR169C-A_p)|FD-Score:4.69|P-value:1.39E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YER039C-A(YER039C-A_p)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:Putative protein of unknown function; YER039C-A is not an essential gene Gene:YER097W(YER097W_d)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER121W(YER121W_p)|FD-Score:5.67|P-value:7.13E-9||SGD DESC:Putative protein of unknown function; may be involved in phosphatase regulation and/or generation of precursor metabolites and energy Gene:YGR125W(YGR125W_p)|FD-Score:-4.16|P-value:1.62E-5||SGD DESC:Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YIR044C(YIR044C)|FD-Score:4.61|P-value:2.05E-6||SGD DESC:Possible pseudogene in strain S288C; YIR044C and the adjacent ORF, YIR043C, together may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family Gene:YLR202C(YLR202C_d)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron Gene:YMR099C(YMR099C)|FD-Score:-3.6|P-value:1.61E-4||SGD DESC:Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase), likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS Gene:YMR253C(YMR253C_p)|FD-Score:4.45|P-value:4.26E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene Gene:YNL200C(YNL200C)|FD-Score:3.28|P-value:5.27E-4||SGD DESC:NADHX epimerase; catalyzes isomerization of (R)- and (S)-NADHX; homologous to AIBP in mammals and the N- terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YNL295W(YNL295W_p)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Putative protein of unknown function Gene:YNR029C(YNR029C_p)|FD-Score:4.37|P-value:6.22E-6||SGD DESC:Putative protein of unknown function, deletion confers reduced fitness in saline Gene:YNR061C(YNR061C_p)|FD-Score:3.17|P-value:7.72E-4||SGD DESC:Protein of unknown function; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:YOL160W(YOL160W_d)|FD-Score:3.24|P-value:5.89E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR020W-A(YOR020W-A_p)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Putative protein of unknown function, conserved in A. gossypii; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOR300W(YOR300W_d)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; overlaps with verified gene BUD7/YOR299W; mutation affects bipolar budding and bud site selection, though phenotype could be due to the mutation's effects on BUD7 Gene:YOS9(YDR057W)|FD-Score:-3.12|P-value:8.95E-4||SGD DESC:ER quality-control lectin; integral subunit of the HRD ligase; binds to glycans with terminal alpha-1,6 linked mannose on misfolded N-glycosylated proteins and participates in targeting proteins to ERAD; member of the OS-9 protein family Gene:YOX1(YML027W)|FD-Score:4.47|P-value:3.86E-6||SGD DESC:Homeobox transcriptional repressor; binds to Mcm1p and to early cell cycle boxes (ECBs) in the promoters of cell cycle-regulated genes expressed in M/G1 phase; expression is cell cycle-regulated; potential Cdc28p substrate; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOX1 has a paralog, YHP1, that arose from the whole genome duplication Gene:YPR077C(YPR077C_d)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression increased by deletion of NAP1

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBL034C5.452.55E-80.63STU1Component of the mitotic spindle that binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles
YMR235C5.181.10E-70.63RNA1GTPase activating protein (GAP) for Gsp1p, involved in nuclear transport
YGR120C5.081.86E-70.63COG2Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YDR398W4.611.98E-60.63UTP5Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit
YDR280W4.493.53E-60.63RRP45Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress
YDR246W3.865.71E-50.03TRS23One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic; human homolog is TRAPPC4
YLR198C_d3.826.59E-50.21YLR198C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W
YDL205C3.611.54E-40.09HEM3Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme
YAL034W-A3.522.15E-40.07MTW1Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly
YHR042W3.452.84E-40.13NCP1NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p
YBR202W3.324.52E-40.01MCM7Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex
YDR236C3.304.75E-40.07FMN1Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane
YIL109C3.246.00E-40.11SEC24Component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb2p and Sfb3p
YPL043W3.138.70E-40.05NOP4Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs)
YDR437W3.080.001030.04GPI19Subunit of GPI-GlcNAc transferase involved in synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), which is the first intermediate in glycosylphosphatidylinositol (GPI) anchor synthesis, shares similarity with mammalian PIG-P

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR133C8.642.81E-18HSL7Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress
YMR256C7.793.44E-15COX7Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain
YDR225W6.183.19E-10HTA1Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p
YPR156C6.076.40E-10TPO3Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; TPO3 has a paralog, TPO2, that arose from the whole genome duplication
YMR322C_p5.951.33E-9SNO4_pPossible chaperone and cysteine protease, similar to bacterial Hsp31 and yeast Hsp31p, Hsp32p, and Hsp33p; DJ-1/ThiJ/PfpI superfamily member; predicted involvement in pyridoxine metabolism; induced by mild heat stress and copper deprivation
YER121W_p5.677.13E-9YER121W_pPutative protein of unknown function; may be involved in phosphatase regulation and/or generation of precursor metabolites and energy
YJL133W5.201.02E-7MRS3Iron transporter, mediates Fe2+ transport across inner mito membrane; mitochondrial carrier family member; active under low-iron conditions; may transport other cations; MRS3 has a paralog, MRS4, that arose from the whole genome duplication
YNL127W4.934.14E-7FAR11Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2
YMR133W4.701.33E-6REC114Protein involved in early stages of meiotic recombination; possibly involved in the coordination of recombination and meiotic division; mutations lead to premature initiation of the first meiotic division
YDR169C-A_p4.691.39E-6YDR169C-A_pPutative protein of unknown function; identified by fungal homology and RT-PCR
YPR001W4.691.39E-6CIT3Dual specificity mitochondrial citrate and methylcitrate synthase; catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and that of propionyl-CoA and oxaloacetate to form 2-methylcitrate
YNL159C4.631.86E-6ASI2Integral inner nuclear membrane protein that acts with Asi1p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals
YIR044C4.612.05E-6YIR044CPossible pseudogene in strain S288C; YIR044C and the adjacent ORF, YIR043C, together may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family
YHR115C4.552.71E-6DMA1Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma2p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8 protein, similar to human Chfr; contains FHA and RING finger
YML027W4.473.86E-6YOX1Homeobox transcriptional repressor; binds to Mcm1p and to early cell cycle boxes (ECBs) in the promoters of cell cycle-regulated genes expressed in M/G1 phase; expression is cell cycle-regulated; potential Cdc28p substrate; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOX1 has a paralog, YHP1, that arose from the whole genome duplication

GO enrichment analysis for SGTC_2692
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2042.82E-56SGTC_2693st077581 40.7 μMTimTec (Natural product derivative library)252387610.106195
0.0758.96E-9SGTC_1832st054113 55.0 μMTimTec (Natural product derivative library)12512670.0606061
0.0655.85E-7SGTC_1915st067105 30.6 μMTimTec (Natural product derivative library)697510.0857143RNA pol III & RNase P/MRP
0.0655.90E-7SGTC_5931203-0218 61.7 μMChemDiv (Drug-like library)36669560.125
0.0612.32E-6SGTC_2748diazoxide 86.7 μMMiscellaneous30190.102564
0.0571.19E-5SGTC_8860866-0150 153.0 μMChemDiv (Drug-like library)2714630.0886076
0.0561.82E-5SGTC_7981287-0182 476.0 μMChemDiv (Drug-like library)16497550.0625
0.0552.56E-5SGTC_25354',4'-dimethoxydalbergione 51.2 μMMicrosource (Natural product library)3641060.0705882DNA damage response
0.0552.73E-5SGTC_2686alizarin 95.4 μMMiscellaneous62930.0666667RPP1 & pyrimidine depletion
0.0534.79E-5SGTC_32519135047 49.5 μMChembridge (Drug-like library)176829630.13186860S ribosome export
0.0526.10E-5SGTC_6104215-0184 155.0 μMChemDiv (Drug-like library)56050150.126316exosome
0.0526.57E-5SGTC_21435623677 140.5 μMChembridge (Fragment library)53753130.0833333
0.0527.46E-5SGTC_20275150920 200.0 μMChembridge (Fragment library)7867150.105882
0.0518.05E-5SGTC_13281441-0025 69.2 μMChemDiv (Drug-like library)45626010.1625
0.0518.95E-5SGTC_9391165-0479 45.3 μMChemDiv (Drug-like library)13773470.0945946

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2709st07856056 μM0.54929617250903TimTec (Natural product derivative library)357.430062.43724
SGTC_2710st07856253.72 μM0.51388917250908TimTec (Natural product derivative library)357.430062.40724
SGTC_2708st07855956 μM0.50684917250902TimTec (Natural product derivative library)357.430062.43724
SGTC_2707st07855666.6 μM0.517250899TimTec (Natural product derivative library)300.378742.89813
SGTC_1944st07637156.9 μM0.47297324208455TimTec (Natural product derivative library)351.3312461.52726
SGTC_1948st07632966.6 μM0.40277824208413TimTec (Natural product derivative library)300.378743.03613
SGTC_1942st07633065.2 μM0.424208414TimTec (Natural product derivative library)306.770642.72813
SGTC_1943st07633165.2 μM0.35135124208415TimTec (Natural product derivative library)306.770642.72813
SGTC_1947st07633261.6 μM0.33333324208416TimTec (Natural product derivative library)324.7611032.93314
SGTC_3297911750271.43 μM0.3108117453720Chembridge (Drug-like library)355.210341.93624