st077718

8-chloro-2,3,4,5-tetrahydro-1H-pyrido[4,3-b]indole

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2700
Screen concentration 96.8 μM
Source TimTec (Natural product derivative library)
PubChem CID 904970
SMILES C1CNCC2=C1NC3=C2C=C(C=C3)Cl
Standardized SMILES Clc1ccc2[nH]c3CCNCc3c2c1
Molecular weight 206.6714
ALogP 2.41
H-bond donor count 2
H-bond acceptor count 1
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.33
% growth inhibition (Hom. pool) 2.36


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 904970
Download HIP data (tab-delimited text)  (excel)
Gene:FAF1(YIL019W)|FD-Score:3.11|P-value:9.24E-4|Clearance:0.08||SGD DESC:Protein required for pre-rRNA processing and 40S ribosomal subunit assembly Gene:GPN2(YOR262W)|FD-Score:-3.55|P-value:1.90E-4|Clearance:0||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion Gene:HEM12(YDR047W)|FD-Score:3.26|P-value:5.61E-4|Clearance:0.11||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:MCM2(YBL023C)|FD-Score:3.14|P-value:8.31E-4|Clearance:0.03||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress Gene:MET4(YNL103W)|FD-Score:-3.66|P-value:1.24E-4|Clearance:0||SGD DESC:Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p Gene:MIM1(YOL026C)|FD-Score:4.02|P-value:2.92E-5|Clearance:0.35||SGD DESC:Mitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; present in a complex with Mim2p in the outer membrane that may create a local environment to facilitate membrane insertion of substrate proteins; also has a role in assembly of Tom20p into the TOM complex Gene:NAM9(YNL137C)|FD-Score:6.06|P-value:6.91E-10|Clearance:0.78||SGD DESC:Mitochondrial ribosomal component of the small subunit Gene:NDC1(YML031W)|FD-Score:3.53|P-value:2.08E-4|Clearance:0.14||SGD DESC:Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1 Gene:RHO1(YPR165W)|FD-Score:5.49|P-value:2.04E-8|Clearance:0.78||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPC11(YDR045C)|FD-Score:4.14|P-value:1.74E-5|Clearance:0.12||SGD DESC:RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS Gene:RVB2(YPL235W)|FD-Score:3.56|P-value:1.86E-4|Clearance:0.03||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SUP35(YDR172W)|FD-Score:4.71|P-value:1.27E-6|Clearance:0.57||SGD DESC:Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype Gene:TIM22(YDL217C)|FD-Score:3.39|P-value:3.45E-4|Clearance:0.14||SGD DESC:Essential core component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported Gene:YAH1(YPL252C)|FD-Score:-4.08|P-value:2.27E-5|Clearance:0||SGD DESC:Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin Gene:YGR073C(YGR073C_d)|FD-Score:3.67|P-value:1.22E-4|Clearance:0.11||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein Gene:FAF1(YIL019W)|FD-Score:3.11|P-value:9.24E-4|Clearance:0.08||SGD DESC:Protein required for pre-rRNA processing and 40S ribosomal subunit assembly Gene:GPN2(YOR262W)|FD-Score:-3.55|P-value:1.90E-4|Clearance:0||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion Gene:HEM12(YDR047W)|FD-Score:3.26|P-value:5.61E-4|Clearance:0.11||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:MCM2(YBL023C)|FD-Score:3.14|P-value:8.31E-4|Clearance:0.03||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress Gene:MET4(YNL103W)|FD-Score:-3.66|P-value:1.24E-4|Clearance:0||SGD DESC:Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p Gene:MIM1(YOL026C)|FD-Score:4.02|P-value:2.92E-5|Clearance:0.35||SGD DESC:Mitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; present in a complex with Mim2p in the outer membrane that may create a local environment to facilitate membrane insertion of substrate proteins; also has a role in assembly of Tom20p into the TOM complex Gene:NAM9(YNL137C)|FD-Score:6.06|P-value:6.91E-10|Clearance:0.78||SGD DESC:Mitochondrial ribosomal component of the small subunit Gene:NDC1(YML031W)|FD-Score:3.53|P-value:2.08E-4|Clearance:0.14||SGD DESC:Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1 Gene:RHO1(YPR165W)|FD-Score:5.49|P-value:2.04E-8|Clearance:0.78||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPC11(YDR045C)|FD-Score:4.14|P-value:1.74E-5|Clearance:0.12||SGD DESC:RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS Gene:RVB2(YPL235W)|FD-Score:3.56|P-value:1.86E-4|Clearance:0.03||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SUP35(YDR172W)|FD-Score:4.71|P-value:1.27E-6|Clearance:0.57||SGD DESC:Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype Gene:TIM22(YDL217C)|FD-Score:3.39|P-value:3.45E-4|Clearance:0.14||SGD DESC:Essential core component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported Gene:YAH1(YPL252C)|FD-Score:-4.08|P-value:2.27E-5|Clearance:0||SGD DESC:Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin Gene:YGR073C(YGR073C_d)|FD-Score:3.67|P-value:1.22E-4|Clearance:0.11||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 904970
Download HOP data (tab-delimited text)  (excel)
Gene:AAP1(YHR047C)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Arginine/alanine amino peptidase; overproduction stimulates glycogen accumulation; AAP1 has a paralog, APE2, that arose from the whole genome duplication Gene:AIM13(YFR011C)|FD-Score:3.24|P-value:5.94E-4||SGD DESC:Mitochondrial protein peripheral to the inner membrane; component of the mitochondrial inner membrane organizing system (MINOS, MitOS, or MICOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:CBT1(YKL208W)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Protein involved in 5' end processing of mitochondrial COB, 15S_rRNA, and RPM1 transcripts; may also have a role in 3' end processing of the COB pre-mRNA; displays genetic interaction with cell cycle-regulated kinase Dbf2p Gene:CPT1(YNL130C)|FD-Score:-5.26|P-value:7.31E-8||SGD DESC:Cholinephosphotransferase; required for phosphatidylcholine biosynthesis and for inositol-dependent regulation of EPT1 transcription; CPT1 has a paralog, EPT1, that arose from the whole genome duplication Gene:CSI1(YMR025W)|FD-Score:3.3|P-value:4.88E-4||SGD DESC:Subunit of the Cop9 signalosome, which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling; functional equivalent of canonical Csn6 subunit of the COP9 signalosome Gene:CTR1(YPR124W)|FD-Score:4.79|P-value:8.47E-7||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CYC7(YEL039C)|FD-Score:3.19|P-value:7.21E-4||SGD DESC:Cytochrome c isoform 2, expressed under hypoxic conditions; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; protein abundance increases in response to DNA replication stress; CYC7 has a paralog, CYC1, that arose from the whole genome duplication Gene:DAK2(YFL053W)|FD-Score:4.04|P-value:2.70E-5||SGD DESC:Dihydroxyacetone kinase, required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation Gene:ECI1(YLR284C)|FD-Score:3.29|P-value:5.00E-4||SGD DESC:Peroxisomal delta3,delta2-enoyl-CoA isomerase, hexameric protein that converts 3-hexenoyl-CoA to trans-2-hexenoyl-CoA, essential for the beta-oxidation of unsaturated fatty acids, oleate-induced Gene:ENT3(YJR125C)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p Gene:FPR4(YLR449W)|FD-Score:3.32|P-value:4.55E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase) (proline isomerase) localized to the nucleus; catalyzes isomerization of proline residues in histones H3 and H4, which affects lysine methylation of those histones Gene:FUN26(YAL022C)|FD-Score:4.56|P-value:2.62E-6||SGD DESC:Vacuolar membrane transporter with broad nucleoside selectivity; may regulate balance of nicotinamide riboside (NmR) levels between cytosol and vacuole, contributing to salvage of NmR for use in cytosolic NAD+ synthesis Gene:HPT1(YDR399W)|FD-Score:-3.78|P-value:7.70E-5||SGD DESC:Dimeric hypoxanthine-guanine phosphoribosyltransferase, catalyzes the transfer of the phosphoribosyl portion of 5-phosphoribosyl-alpha-1-pyrophosphate to a purine base (either guanine or hypoxanthine) to form pyrophosphate and a purine nucleotide (either guanosine monophosphate or inosine monophosphate); mutations in the human homolog HPRT1 can cause Lesch-Nyhan syndrome and Kelley-Seegmiller syndrome Gene:ILM1(YJR118C)|FD-Score:-3.85|P-value:5.95E-5||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:MDM12(YOL009C)|FD-Score:5.04|P-value:2.28E-7||SGD DESC:Mitochondrial outer membrane protein, required for transmission of mitochondria to daughter cells; component of the ERMES complex that links the ER to mitochondria; may influence import and assembly of outer membrane beta-barrel proteins Gene:MET28(YIR017C)|FD-Score:-3.72|P-value:9.90E-5||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator in the Cbf1p-Met4p-Met28p complex, participates in the regulation of sulfur metabolism Gene:MOH1(YBL049W)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase Gene:MRPL16(YBL038W)|FD-Score:4.69|P-value:1.39E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NNF2(YGR089W)|FD-Score:3.6|P-value:1.56E-4||SGD DESC:Protein that exhibits physical and genetic interactions with Rpb8p, which is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation Gene:PAC1(YOR269W)|FD-Score:-3.99|P-value:3.32E-5||SGD DESC:Involved in nuclear migration, part of the dynein/dynactin pathway; targets dynein to microtubule tips, which is necessary for sliding of microtubules along bud cortex; serves at interface between dynein's ATPase site and its microtubule binding stalk, causing individual dynein motors to remain attached to microtubules for long periods; synthetic lethal with bni1; homolog of human LIS1, mutations in which cause the severe brain disorder lissencephaly Gene:PAU17(YLL025W)|FD-Score:-4.11|P-value:1.96E-5||SGD DESC:Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; YLL025W is not an essential gene Gene:PCL9(YDL179W)|FD-Score:4.9|P-value:4.88E-7||SGD DESC:Cyclin, forms a functional kinase complex with Pho85p cyclin-dependent kinase (Cdk), expressed in late M/early G1 phase, activated by Swi5p Gene:PEP3(YLR148W)|FD-Score:-3.82|P-value:6.77E-5||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PEX30(YLR324W)|FD-Score:-3.12|P-value:9.11E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PIR3(YKL163W)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:O-glycosylated covalently-bound cell wall protein; required for cell wall stability; expression is cell cycle regulated, peaking in M/G1 and also subject to regulation by the cell integrity pathway; PIR3 has a paralog, HSP150, that arose from the whole genome duplication Gene:PYK2(YOR347C)|FD-Score:-3.38|P-value:3.60E-4||SGD DESC:Pyruvate kinase; appears to be modulated by phosphorylation; transcription repressed by glucose, and Pyk2p may be active under low glycolytic flux; PYK2 has a paralog, CDC19, that arose from the whole genome duplication Gene:RAS2(YNL098C)|FD-Score:3.3|P-value:4.79E-4||SGD DESC:GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes Gene:RBK1(YCR036W)|FD-Score:3.73|P-value:9.50E-5||SGD DESC:Putative ribokinase Gene:RPL13B(YMR142C)|FD-Score:-3.73|P-value:9.70E-5||SGD DESC:Ribosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication Gene:RPL29(YFR032C-A)|FD-Score:-3.34|P-value:4.15E-4||SGD DESC:Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog Gene:RPL31B(YLR406C)|FD-Score:4.43|P-value:4.69E-6||SGD DESC:Ribosomal 60S subunit protein L31B; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31B has a paralog, RPL31A, that arose from the whole genome duplication Gene:RPS16A(YMR143W)|FD-Score:3.93|P-value:4.30E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16A has a paralog, RPS16B, that arose from the whole genome duplication Gene:RPS1B(YML063W)|FD-Score:-3.6|P-value:1.60E-4||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1B has a paralog, RPS1A, that arose from the whole genome duplication Gene:RTC1(YOL138C)|FD-Score:-3.13|P-value:8.69E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; null mutation suppresses cdc13-1 temperature sensitivity; has N-terminal WD-40 repeats and a C-terminal RING motif Gene:SAN1(YDR143C)|FD-Score:-3.68|P-value:1.18E-4||SGD DESC:Ubiquitin-protein ligase; involved in the proteasome-dependent degradation of aberrant nuclear proteins; targets substrates with regions of exposed hydrophobicity containing 5 or more contiguous hydrophobic residues; contains intrinsically disordered regions that contribute to substrate recognition Gene:SEG1(YMR086W)|FD-Score:-3.3|P-value:4.76E-4||SGD DESC:Component of eisosome required for proper eisosome assembly; precedes Pil1p/Lsp1p during eisosome formation and controls eisosome length and shape; Seg1p-GFP diffusely distributed and forms heterogeneous patches at the plasma membrane in small buds, also found in medium and large buds; may interact with ribosomes, based on co-purification experiments; expression is repressed by cAMP; similar to A. gossypii SEG gene and to S. pombe Sle1p, important for generating eisosomes Gene:SLG1(YOR008C)|FD-Score:3.2|P-value:6.77E-4||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SLS1(YLR139C)|FD-Score:-3.54|P-value:2.02E-4||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SLT2(YHR030C)|FD-Score:3.17|P-value:7.64E-4||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SWA2(YDR320C)|FD-Score:-3.09|P-value:9.89E-4||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:SWT1(YOR166C)|FD-Score:-3.49|P-value:2.41E-4||SGD DESC:RNA endoribonuclease involved in perinuclear mRNP quality control via the turnover of aberrant, unprocessed pre-mRNAs; interacts with subunits of THO/TREX, TREX-2, and RNA polymerase II; contains a PIN (PilT N terminus) domain Gene:SYT1(YPR095C)|FD-Score:-3.23|P-value:6.24E-4||SGD DESC:Guanine nucleotide exchange factor (GEF) for Arf proteins; promotes activation of Arl1p, which recruits Imh1p to the Golgi; involved in vesicular transport; member of the Sec7-domain family; contains a PH domain Gene:TPN1(YGL186C)|FD-Score:3.27|P-value:5.34E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRP2(YER090W)|FD-Score:-3.24|P-value:6.06E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UTR1(YJR049C)|FD-Score:-4.09|P-value:2.13E-5||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:VPS1(YKR001C)|FD-Score:-3.61|P-value:1.56E-4||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS21(YOR089C)|FD-Score:-3.74|P-value:9.20E-5||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS27(YNR006W)|FD-Score:-3.24|P-value:5.97E-4||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:VPS60(YDR486C)|FD-Score:-3.81|P-value:6.90E-5||SGD DESC:Cytoplasmic and vacuolar membrane protein involved in late endosome to vacuole transport; required for normal filament maturation during pseudohyphal growth; may function in targeting cargo proteins for degradation; interacts with Vta1p Gene:VPS75(YNL246W)|FD-Score:4.17|P-value:1.51E-5||SGD DESC:NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress Gene:YAP7(YOL028C)|FD-Score:3.82|P-value:6.78E-5||SGD DESC:Putative basic leucine zipper (bZIP) transcription factor; YAP7 has a paralog, YAP5, that arose from the whole genome duplication Gene:YCK1(YHR135C)|FD-Score:3.79|P-value:7.68E-5||SGD DESC:Palmitoylated plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck2p in morphogenesis, proper septin assembly, endocytic trafficking; YCK1 has a paralog, YCK2, that arose from the whole genome duplication Gene:YCR016W(YCR016W_p)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis Gene:YCR025C(YCR025C_d)|FD-Score:5.05|P-value:2.17E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR025C is not an essential gene Gene:YEL014C(YEL014C_d)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFR012W-A(YFR012W-A_p)|FD-Score:4.18|P-value:1.48E-5||SGD DESC:Putative protein of unknown function; identified by homology Gene:YGL230C(YGL230C_p)|FD-Score:3.14|P-value:8.53E-4||SGD DESC:Putative protein of unknown function; non-essential gene Gene:YLR297W(YLR297W_p)|FD-Score:-3.86|P-value:5.61E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; not an essential gene; induced by treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from nucleus to vacuole upon DNA replication stress; YLR297W has a paralog, YOR186W, that arose from the whole genome duplication Gene:YML089C(YML089C_d)|FD-Score:3.21|P-value:6.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression induced by calcium shortage Gene:YNL184C(YNL184C_p)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOR022C(YOR022C_p)|FD-Score:4.76|P-value:9.56E-7||SGD DESC:Putative carboxylic ester hydrolase; similar to bovine phospholipase A1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOR053W(YOR053W_d)|FD-Score:4.44|P-value:4.45E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VHS3/YOR054C Gene:YPL073C(YPL073C_d)|FD-Score:-3.78|P-value:7.84E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with verified gene UBP16/YPL072W; may interact with ribosome based on co-purification experiments Gene:YPS3(YLR121C)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:AAP1(YHR047C)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Arginine/alanine amino peptidase; overproduction stimulates glycogen accumulation; AAP1 has a paralog, APE2, that arose from the whole genome duplication Gene:AIM13(YFR011C)|FD-Score:3.24|P-value:5.94E-4||SGD DESC:Mitochondrial protein peripheral to the inner membrane; component of the mitochondrial inner membrane organizing system (MINOS, MitOS, or MICOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:CBT1(YKL208W)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Protein involved in 5' end processing of mitochondrial COB, 15S_rRNA, and RPM1 transcripts; may also have a role in 3' end processing of the COB pre-mRNA; displays genetic interaction with cell cycle-regulated kinase Dbf2p Gene:CPT1(YNL130C)|FD-Score:-5.26|P-value:7.31E-8||SGD DESC:Cholinephosphotransferase; required for phosphatidylcholine biosynthesis and for inositol-dependent regulation of EPT1 transcription; CPT1 has a paralog, EPT1, that arose from the whole genome duplication Gene:CSI1(YMR025W)|FD-Score:3.3|P-value:4.88E-4||SGD DESC:Subunit of the Cop9 signalosome, which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling; functional equivalent of canonical Csn6 subunit of the COP9 signalosome Gene:CTR1(YPR124W)|FD-Score:4.79|P-value:8.47E-7||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CYC7(YEL039C)|FD-Score:3.19|P-value:7.21E-4||SGD DESC:Cytochrome c isoform 2, expressed under hypoxic conditions; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; protein abundance increases in response to DNA replication stress; CYC7 has a paralog, CYC1, that arose from the whole genome duplication Gene:DAK2(YFL053W)|FD-Score:4.04|P-value:2.70E-5||SGD DESC:Dihydroxyacetone kinase, required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation Gene:ECI1(YLR284C)|FD-Score:3.29|P-value:5.00E-4||SGD DESC:Peroxisomal delta3,delta2-enoyl-CoA isomerase, hexameric protein that converts 3-hexenoyl-CoA to trans-2-hexenoyl-CoA, essential for the beta-oxidation of unsaturated fatty acids, oleate-induced Gene:ENT3(YJR125C)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p Gene:FPR4(YLR449W)|FD-Score:3.32|P-value:4.55E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase) (proline isomerase) localized to the nucleus; catalyzes isomerization of proline residues in histones H3 and H4, which affects lysine methylation of those histones Gene:FUN26(YAL022C)|FD-Score:4.56|P-value:2.62E-6||SGD DESC:Vacuolar membrane transporter with broad nucleoside selectivity; may regulate balance of nicotinamide riboside (NmR) levels between cytosol and vacuole, contributing to salvage of NmR for use in cytosolic NAD+ synthesis Gene:HPT1(YDR399W)|FD-Score:-3.78|P-value:7.70E-5||SGD DESC:Dimeric hypoxanthine-guanine phosphoribosyltransferase, catalyzes the transfer of the phosphoribosyl portion of 5-phosphoribosyl-alpha-1-pyrophosphate to a purine base (either guanine or hypoxanthine) to form pyrophosphate and a purine nucleotide (either guanosine monophosphate or inosine monophosphate); mutations in the human homolog HPRT1 can cause Lesch-Nyhan syndrome and Kelley-Seegmiller syndrome Gene:ILM1(YJR118C)|FD-Score:-3.85|P-value:5.95E-5||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:MDM12(YOL009C)|FD-Score:5.04|P-value:2.28E-7||SGD DESC:Mitochondrial outer membrane protein, required for transmission of mitochondria to daughter cells; component of the ERMES complex that links the ER to mitochondria; may influence import and assembly of outer membrane beta-barrel proteins Gene:MET28(YIR017C)|FD-Score:-3.72|P-value:9.90E-5||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator in the Cbf1p-Met4p-Met28p complex, participates in the regulation of sulfur metabolism Gene:MOH1(YBL049W)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase Gene:MRPL16(YBL038W)|FD-Score:4.69|P-value:1.39E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NNF2(YGR089W)|FD-Score:3.6|P-value:1.56E-4||SGD DESC:Protein that exhibits physical and genetic interactions with Rpb8p, which is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation Gene:PAC1(YOR269W)|FD-Score:-3.99|P-value:3.32E-5||SGD DESC:Involved in nuclear migration, part of the dynein/dynactin pathway; targets dynein to microtubule tips, which is necessary for sliding of microtubules along bud cortex; serves at interface between dynein's ATPase site and its microtubule binding stalk, causing individual dynein motors to remain attached to microtubules for long periods; synthetic lethal with bni1; homolog of human LIS1, mutations in which cause the severe brain disorder lissencephaly Gene:PAU17(YLL025W)|FD-Score:-4.11|P-value:1.96E-5||SGD DESC:Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; YLL025W is not an essential gene Gene:PCL9(YDL179W)|FD-Score:4.9|P-value:4.88E-7||SGD DESC:Cyclin, forms a functional kinase complex with Pho85p cyclin-dependent kinase (Cdk), expressed in late M/early G1 phase, activated by Swi5p Gene:PEP3(YLR148W)|FD-Score:-3.82|P-value:6.77E-5||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PEX30(YLR324W)|FD-Score:-3.12|P-value:9.11E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PIR3(YKL163W)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:O-glycosylated covalently-bound cell wall protein; required for cell wall stability; expression is cell cycle regulated, peaking in M/G1 and also subject to regulation by the cell integrity pathway; PIR3 has a paralog, HSP150, that arose from the whole genome duplication Gene:PYK2(YOR347C)|FD-Score:-3.38|P-value:3.60E-4||SGD DESC:Pyruvate kinase; appears to be modulated by phosphorylation; transcription repressed by glucose, and Pyk2p may be active under low glycolytic flux; PYK2 has a paralog, CDC19, that arose from the whole genome duplication Gene:RAS2(YNL098C)|FD-Score:3.3|P-value:4.79E-4||SGD DESC:GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes Gene:RBK1(YCR036W)|FD-Score:3.73|P-value:9.50E-5||SGD DESC:Putative ribokinase Gene:RPL13B(YMR142C)|FD-Score:-3.73|P-value:9.70E-5||SGD DESC:Ribosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication Gene:RPL29(YFR032C-A)|FD-Score:-3.34|P-value:4.15E-4||SGD DESC:Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog Gene:RPL31B(YLR406C)|FD-Score:4.43|P-value:4.69E-6||SGD DESC:Ribosomal 60S subunit protein L31B; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31B has a paralog, RPL31A, that arose from the whole genome duplication Gene:RPS16A(YMR143W)|FD-Score:3.93|P-value:4.30E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16A has a paralog, RPS16B, that arose from the whole genome duplication Gene:RPS1B(YML063W)|FD-Score:-3.6|P-value:1.60E-4||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1B has a paralog, RPS1A, that arose from the whole genome duplication Gene:RTC1(YOL138C)|FD-Score:-3.13|P-value:8.69E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; null mutation suppresses cdc13-1 temperature sensitivity; has N-terminal WD-40 repeats and a C-terminal RING motif Gene:SAN1(YDR143C)|FD-Score:-3.68|P-value:1.18E-4||SGD DESC:Ubiquitin-protein ligase; involved in the proteasome-dependent degradation of aberrant nuclear proteins; targets substrates with regions of exposed hydrophobicity containing 5 or more contiguous hydrophobic residues; contains intrinsically disordered regions that contribute to substrate recognition Gene:SEG1(YMR086W)|FD-Score:-3.3|P-value:4.76E-4||SGD DESC:Component of eisosome required for proper eisosome assembly; precedes Pil1p/Lsp1p during eisosome formation and controls eisosome length and shape; Seg1p-GFP diffusely distributed and forms heterogeneous patches at the plasma membrane in small buds, also found in medium and large buds; may interact with ribosomes, based on co-purification experiments; expression is repressed by cAMP; similar to A. gossypii SEG gene and to S. pombe Sle1p, important for generating eisosomes Gene:SLG1(YOR008C)|FD-Score:3.2|P-value:6.77E-4||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SLS1(YLR139C)|FD-Score:-3.54|P-value:2.02E-4||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SLT2(YHR030C)|FD-Score:3.17|P-value:7.64E-4||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SWA2(YDR320C)|FD-Score:-3.09|P-value:9.89E-4||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:SWT1(YOR166C)|FD-Score:-3.49|P-value:2.41E-4||SGD DESC:RNA endoribonuclease involved in perinuclear mRNP quality control via the turnover of aberrant, unprocessed pre-mRNAs; interacts with subunits of THO/TREX, TREX-2, and RNA polymerase II; contains a PIN (PilT N terminus) domain Gene:SYT1(YPR095C)|FD-Score:-3.23|P-value:6.24E-4||SGD DESC:Guanine nucleotide exchange factor (GEF) for Arf proteins; promotes activation of Arl1p, which recruits Imh1p to the Golgi; involved in vesicular transport; member of the Sec7-domain family; contains a PH domain Gene:TPN1(YGL186C)|FD-Score:3.27|P-value:5.34E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRP2(YER090W)|FD-Score:-3.24|P-value:6.06E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UTR1(YJR049C)|FD-Score:-4.09|P-value:2.13E-5||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:VPS1(YKR001C)|FD-Score:-3.61|P-value:1.56E-4||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS21(YOR089C)|FD-Score:-3.74|P-value:9.20E-5||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS27(YNR006W)|FD-Score:-3.24|P-value:5.97E-4||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:VPS60(YDR486C)|FD-Score:-3.81|P-value:6.90E-5||SGD DESC:Cytoplasmic and vacuolar membrane protein involved in late endosome to vacuole transport; required for normal filament maturation during pseudohyphal growth; may function in targeting cargo proteins for degradation; interacts with Vta1p Gene:VPS75(YNL246W)|FD-Score:4.17|P-value:1.51E-5||SGD DESC:NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress Gene:YAP7(YOL028C)|FD-Score:3.82|P-value:6.78E-5||SGD DESC:Putative basic leucine zipper (bZIP) transcription factor; YAP7 has a paralog, YAP5, that arose from the whole genome duplication Gene:YCK1(YHR135C)|FD-Score:3.79|P-value:7.68E-5||SGD DESC:Palmitoylated plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck2p in morphogenesis, proper septin assembly, endocytic trafficking; YCK1 has a paralog, YCK2, that arose from the whole genome duplication Gene:YCR016W(YCR016W_p)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis Gene:YCR025C(YCR025C_d)|FD-Score:5.05|P-value:2.17E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR025C is not an essential gene Gene:YEL014C(YEL014C_d)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFR012W-A(YFR012W-A_p)|FD-Score:4.18|P-value:1.48E-5||SGD DESC:Putative protein of unknown function; identified by homology Gene:YGL230C(YGL230C_p)|FD-Score:3.14|P-value:8.53E-4||SGD DESC:Putative protein of unknown function; non-essential gene Gene:YLR297W(YLR297W_p)|FD-Score:-3.86|P-value:5.61E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; not an essential gene; induced by treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from nucleus to vacuole upon DNA replication stress; YLR297W has a paralog, YOR186W, that arose from the whole genome duplication Gene:YML089C(YML089C_d)|FD-Score:3.21|P-value:6.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression induced by calcium shortage Gene:YNL184C(YNL184C_p)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOR022C(YOR022C_p)|FD-Score:4.76|P-value:9.56E-7||SGD DESC:Putative carboxylic ester hydrolase; similar to bovine phospholipase A1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOR053W(YOR053W_d)|FD-Score:4.44|P-value:4.45E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VHS3/YOR054C Gene:YPL073C(YPL073C_d)|FD-Score:-3.78|P-value:7.84E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with verified gene UBP16/YPL072W; may interact with ribosome based on co-purification experiments Gene:YPS3(YLR121C)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YNL137C6.066.91E-100.78NAM9Mitochondrial ribosomal component of the small subunit
YPR165W5.492.04E-80.78RHO1GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
YDR172W4.711.27E-60.56SUP35Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype
YDR045C4.141.74E-50.12RPC11RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS
YOL026C4.022.92E-50.35MIM1Mitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; present in a complex with Mim2p in the outer membrane that may create a local environment to facilitate membrane insertion of substrate proteins; also has a role in assembly of Tom20p into the TOM complex
YGR073C_d3.671.22E-40.11YGR073C_dDubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein
YPL235W3.561.86E-40.03RVB2ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly
YML031W3.532.08E-40.14NDC1Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1
YDL217C3.393.45E-40.14TIM22Essential core component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported
YDR047W3.265.61E-40.11HEM12Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
YBL023C3.148.31E-40.03MCM2Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress
YIL019W3.119.24E-40.08FAF1Protein required for pre-rRNA processing and 40S ribosomal subunit assembly
YGR005C3.030.001200.05TFG2TFIIF (Transcription Factor II) middle subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP30
YDL120W2.980.001440.06YFH1Mitochondrial matrix iron chaperone; oxidizes and stores iron; interacts with Isu1p to promote Fe-S cluster assembly; mutation results in multiple Fe/S-dependent enzyme deficiencies; human frataxin homolog is mutated in Friedrich's ataxia
YKR037C2.920.001740.06SPC34Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YCR025C_d5.052.17E-7YCR025C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR025C is not an essential gene
YOL009C5.042.28E-7MDM12Mitochondrial outer membrane protein, required for transmission of mitochondria to daughter cells; component of the ERMES complex that links the ER to mitochondria; may influence import and assembly of outer membrane beta-barrel proteins
YDL179W4.904.88E-7PCL9Cyclin, forms a functional kinase complex with Pho85p cyclin-dependent kinase (Cdk), expressed in late M/early G1 phase, activated by Swi5p
YPR124W4.798.47E-7CTR1High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress
YOR022C_p4.769.56E-7YOR022C_pPutative carboxylic ester hydrolase; similar to bovine phospholipase A1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YBL038W4.691.39E-6MRPL16Mitochondrial ribosomal protein of the large subunit
YAL022C4.562.62E-6FUN26Vacuolar membrane transporter with broad nucleoside selectivity; may regulate balance of nicotinamide riboside (NmR) levels between cytosol and vacuole, contributing to salvage of NmR for use in cytosolic NAD+ synthesis
YOR053W_d4.444.45E-6YOR053W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VHS3/YOR054C
YLR406C4.434.69E-6RPL31BRibosomal 60S subunit protein L31B; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31B has a paralog, RPL31A, that arose from the whole genome duplication
YBL049W4.191.40E-5MOH1Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase
YFR012W-A_p4.181.48E-5YFR012W-A_pPutative protein of unknown function; identified by homology
YNL246W4.171.51E-5VPS75NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress
YFL053W4.042.70E-5DAK2Dihydroxyacetone kinase, required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation
YMR143W3.934.30E-5RPS16AProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16A has a paralog, RPS16B, that arose from the whole genome duplication
YOL028C3.826.78E-5YAP7Putative basic leucine zipper (bZIP) transcription factor; YAP7 has a paralog, YAP5, that arose from the whole genome duplication

GO enrichment analysis for SGTC_2700
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1096.03E-17SGTC_9052-aminofluorene 215.0 μMChemDiv (Drug-like library)15390.1cell wall signaling
0.1024.50E-15SGTC_32859112818 20.9 μMChembridge (Drug-like library)170179470.0945946cell wall
0.0961.36E-13SGTC_20285153890 36.4 μMChembridge (Fragment library)28309680.113208cell wall signaling
0.0953.18E-13SGTC_21495554786 194.8 μMChembridge (Fragment library)7450630.0645161cell wall signaling
0.0899.15E-12SGTC_33229139058 2.1 μMChembridge (Drug-like library)49089260.0512821cell wall
0.0881.18E-11SGTC_8830850-0245 24.6 μMChemDiv (Drug-like library)68232290.0533333cell wall
0.0881.40E-11SGTC_3600957-0399 1.0 μMChemDiv (Drug-like library)67523010.0487805cell wall
0.0881.53E-11SGTC_33569151811 4.5 μMChembridge (Drug-like library)18592540.05cell wall
0.0857.49E-11SGTC_22727805186 180.0 μMChembridge (Fragment library)29560620.0461538cell wall signaling
0.0814.22E-10SGTC_33509150499 3.6 μMChembridge (Drug-like library)49109960.0365854cell wall
0.0791.03E-9SGTC_29249010168 62.8 μMChembridge (Drug-like library)29939290.0597015cell wall signaling
0.0791.59E-9SGTC_5860957-0265 8.2 μMChemDiv (Drug-like library)67523090.0470588cell wall
0.0791.51E-9SGTC_483fpl-64176 144.0 μMICCB bioactive library34230.0757576cell wall
0.0756.61E-9SGTC_6631486-0733 13.9 μMChemDiv (Drug-like library)7224450.08cell wall signaling
0.0758.10E-9SGTC_28909046729 58.4 μMChembridge (Drug-like library)9033290.131148Golgi

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2702st07776896.8 μM0.523816484119TimTec (Natural product derivative library)206.671442.40521
SGTC_1957st07703562.4 μM0.425926705593TimTec (Natural product derivative library)278.73413.19212
SGTC_2414st07723292.9 μM0.354167729801TimTec (Natural product derivative library)215.247823.13622
SGTC_2696st07769174.32 μM0.326531757133TimTec (Natural product derivative library)200.279542.83611
SGTC_1885st05754699.9 μM0.32702008TimTec (Natural product derivative library)200.236482.18321
SGTC_1952st07703664.1 μM0.2881362937007TimTec (Natural product derivative library)258.315623.01412
SGTC_1793st04825672.23 μM0.278689672022TimTec (Natural product derivative library)274.315022.51113
SGTC_1670st0165954.04 μM0.269231597879TimTec (Natural product derivative library)265.149023.17821
SGTC_1720st0372989.48 μM0.269231597396TimTec (Natural product derivative library)266.133783.46721
SGTC_3660181-02064.31 μM0.25274874ChemDiv (Drug-like library)324.998684.8210Golgi