st078239

4-(pyrrolidine-1-carbonyl)-1H-quinolin-2-one

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2706
Screen concentration 82.5 μM
Source TimTec (Natural product derivative library)
PubChem CID 15434492
SMILES C1CCN(C1)C(=O)C2=CC(=O)NC3=CC=CC=C32
Standardized SMILES Oc1cc(C(=O)N2CCCC2)c3ccccc3n1
Molecular weight 242.2732
ALogP 1.09
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.51
% growth inhibition (Hom. pool) 1.18


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 15434492
Download HIP data (tab-delimited text)  (excel)
Gene:ARP9(YMR033W)|FD-Score:3.5|P-value:2.33E-4|Clearance:0.13||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:HSH49(YOR319W)|FD-Score:3.13|P-value:8.68E-4|Clearance:0.04||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:MEC1(YBR136W)|FD-Score:3.2|P-value:6.95E-4|Clearance:0.03||SGD DESC:Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres Gene:PMI40(YER003C)|FD-Score:4.81|P-value:7.50E-7|Clearance:0.52||SGD DESC:Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation Gene:PRE8(YML092C)|FD-Score:-3.43|P-value:3.03E-4|Clearance:0||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:PZF1(YPR186C)|FD-Score:-3.33|P-value:4.37E-4|Clearance:0||SGD DESC:Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA Gene:RFC1(YOR217W)|FD-Score:3.09|P-value:9.99E-4|Clearance:0.17||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:SAD1(YFR005C)|FD-Score:3.33|P-value:4.40E-4|Clearance:0||SGD DESC:Conserved zinc-finger domain protein involved in pre-mRNA splicing, required for assembly of U4 snRNA into the U4/U6 particle Gene:SAM35(YHR083W)|FD-Score:4.29|P-value:9.03E-6|Clearance:0.45||SGD DESC:Essential component of the sorting and assembly machinery (SAM complex or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane Gene:SEC53(YFL045C)|FD-Score:3.16|P-value:7.80E-4|Clearance:0.03||SGD DESC:Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen Gene:STH1(YIL126W)|FD-Score:3.32|P-value:4.47E-4|Clearance:0.13||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:TCP1(YDR212W)|FD-Score:3.84|P-value:6.14E-5|Clearance:0.34||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:TFB2(YPL122C)|FD-Score:3.36|P-value:3.84E-4|Clearance:0.04||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH Gene:TRR1(YDR353W)|FD-Score:-3.35|P-value:4.09E-4|Clearance:0||SGD DESC:Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication Gene:ARP9(YMR033W)|FD-Score:3.5|P-value:2.33E-4|Clearance:0.13||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:HSH49(YOR319W)|FD-Score:3.13|P-value:8.68E-4|Clearance:0.04||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:MEC1(YBR136W)|FD-Score:3.2|P-value:6.95E-4|Clearance:0.03||SGD DESC:Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres Gene:PMI40(YER003C)|FD-Score:4.81|P-value:7.50E-7|Clearance:0.52||SGD DESC:Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation Gene:PRE8(YML092C)|FD-Score:-3.43|P-value:3.03E-4|Clearance:0||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:PZF1(YPR186C)|FD-Score:-3.33|P-value:4.37E-4|Clearance:0||SGD DESC:Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA Gene:RFC1(YOR217W)|FD-Score:3.09|P-value:9.99E-4|Clearance:0.17||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:SAD1(YFR005C)|FD-Score:3.33|P-value:4.40E-4|Clearance:0||SGD DESC:Conserved zinc-finger domain protein involved in pre-mRNA splicing, required for assembly of U4 snRNA into the U4/U6 particle Gene:SAM35(YHR083W)|FD-Score:4.29|P-value:9.03E-6|Clearance:0.45||SGD DESC:Essential component of the sorting and assembly machinery (SAM complex or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane Gene:SEC53(YFL045C)|FD-Score:3.16|P-value:7.80E-4|Clearance:0.03||SGD DESC:Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen Gene:STH1(YIL126W)|FD-Score:3.32|P-value:4.47E-4|Clearance:0.13||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:TCP1(YDR212W)|FD-Score:3.84|P-value:6.14E-5|Clearance:0.34||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:TFB2(YPL122C)|FD-Score:3.36|P-value:3.84E-4|Clearance:0.04||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH Gene:TRR1(YDR353W)|FD-Score:-3.35|P-value:4.09E-4|Clearance:0||SGD DESC:Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 15434492
Download HOP data (tab-delimited text)  (excel)
Gene:AVL9(YLR114C)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:BSC1(YDL037C)|FD-Score:3.09|P-value:9.86E-4||SGD DESC:Protein of unconfirmed function, similar to cell surface flocculin Flo11p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression Gene:CBT1(YKL208W)|FD-Score:-3.51|P-value:2.22E-4||SGD DESC:Protein involved in 5' end processing of mitochondrial COB, 15S_rRNA, and RPM1 transcripts; may also have a role in 3' end processing of the COB pre-mRNA; displays genetic interaction with cell cycle-regulated kinase Dbf2p Gene:COS1(YNL336W)|FD-Score:4.87|P-value:5.45E-7||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:CSM3(YMR048W)|FD-Score:-3.71|P-value:1.04E-4||SGD DESC:Replication fork associated factor; required for stable replication fork pausing; component of the DNA replication checkpoint pathway; required for accurate chromosome segregation during meiosis; forms nuclear foci upon DNA replication stress Gene:CYB5(YNL111C)|FD-Score:3.97|P-value:3.62E-5||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:DAS1(YJL149W)|FD-Score:3.89|P-value:5.06E-5||SGD DESC:Putative SCF ubiquitin ligase F-box protein; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C Gene:DEG1(YFL001W)|FD-Score:4.25|P-value:1.06E-5||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability Gene:DYN2(YDR424C)|FD-Score:3.84|P-value:6.26E-5||SGD DESC:Cytoplasmic light chain dynein, microtubule motor protein; proposed to be involved in the assembly of the nuclear pore complex Gene:EFT1(YOR133W)|FD-Score:-4.1|P-value:2.06E-5||SGD DESC:Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication Gene:FRD1(YEL047C)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Soluble fumarate reductase; required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to Arxula adeninovorans fumarate reductase; protein abundance increases in response to DNA replication stress; FRD1 has a paralog, OSM1, that arose from the whole genome duplication Gene:FYV4(YHR059W)|FD-Score:4.2|P-value:1.33E-5||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GET3(YDL100C)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GUT1(YHL032C)|FD-Score:3.76|P-value:8.38E-5||SGD DESC:Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p Gene:HXT1(YHR094C)|FD-Score:4.33|P-value:7.53E-6||SGD DESC:Low-affinity glucose transporter of the major facilitator superfamily, expression is induced by Hxk2p in the presence of glucose and repressed by Rgt1p when glucose is limiting; HXT1 has a paralog, HXT6, what arose from the whole genome duplication Gene:IFA38(YBR159W)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides Gene:IRC21(YMR073C)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:JLP2(YMR132C_p)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:Protein of unknown function, contains sequence that closely resembles a J domain (typified by the E. coli DnaJ protein) Gene:KCS1(YDR017C)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; generation of high energy inositol pyrophosphates by Kcs1p is required for many processes such as vacuolar biogenesis, stress response and telomere maintenance Gene:LAS21(YJL062W)|FD-Score:3.15|P-value:8.20E-4||SGD DESC:Integral plasma membrane protein involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity Gene:LSM1(YJL124C)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:MEU1(YLR017W)|FD-Score:3.12|P-value:9.15E-4||SGD DESC:Methylthioadenosine phosphorylase (MTAP), catalyzes the initial step in the methionine salvage pathway; affects polyamine biosynthesis through regulation of ornithine decarboxylase (Spe1p) activity; regulates ADH2 gene expression Gene:MID2(YLR332W)|FD-Score:3.37|P-value:3.82E-4||SGD DESC:O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p Gene:MKK2(YPL140C)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk1p; MKK2 has a paralog, MKK1, that arose from the whole genome duplication Gene:MSC6(YOR354C)|FD-Score:3.28|P-value:5.28E-4||SGD DESC:Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:MTC7(YEL033W_p)|FD-Score:4.31|P-value:8.33E-6||SGD DESC:Predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant Gene:PAN3(YKL025C)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PNG1(YPL096W)|FD-Score:3.2|P-value:6.83E-4||SGD DESC:Conserved peptide N-glycanase required for deglycosylation of misfolded glycoproteins during proteasome-dependent degradation; localizes to the cytoplasm and nucleus; activity is enhanced by interaction with Rad23p Gene:PPZ2(YDR436W)|FD-Score:-3.23|P-value:6.09E-4||SGD DESC:Serine/threonine protein phosphatase Z, isoform of Ppz1p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance Gene:PRS3(YHL011C)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:PUF4(YGL014W)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors Gene:RFM1(YOR279C)|FD-Score:-3.11|P-value:9.46E-4||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RGS2(YOR107W)|FD-Score:3.15|P-value:8.04E-4||SGD DESC:Negative regulator of glucose-induced cAMP signaling; directly activates the GTPase activity of the heterotrimeric G protein alpha subunit Gpa2p Gene:RLM1(YPL089C)|FD-Score:4.13|P-value:1.83E-5||SGD DESC:MADS-box transcription factor; component of the protein kinase C-mediated MAP kinase pathway involved in the maintenance of cell integrity; phosphorylated and activated by the MAP-kinase Slt2p; RLM1 has a paralog, SMP1, that arose from the whole genome duplication Gene:RTN2(YDL204W)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; much less abundant than Rtn1p; rtn1 rtn2 yop1 triple mutant lacks tubular ER; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress; RTN2 has a paralog, RTN1, that arose from the whole genome duplication Gene:SAM4(YPL273W)|FD-Score:-3.24|P-value:6.00E-4||SGD DESC:S-adenosylmethionine-homocysteine methyltransferase, functions along with Mht1p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio Gene:SLM2(YNL047C)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm1p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; SLM2 has a paralog, SLM1, that arose from the whole genome duplication Gene:SMA1(YPL027W)|FD-Score:-3.19|P-value:7.01E-4||SGD DESC:Protein of unknown function involved in the assembly of the prospore membrane during sporulation; interacts with Spo14p Gene:SNL1(YIL016W)|FD-Score:-3.13|P-value:8.71E-4||SGD DESC:Ribosome-associated protein proposed to act in protein synthesis and nuclear pore complex biogenesis and maintenance as well as protein folding; has similarity to the mammalian BAG-1 protein Gene:SUR7(YML052W)|FD-Score:-4.39|P-value:5.54E-6||SGD DESC:Plasma membrane protein that localizes to furrow-like invaginations (MCC patches); component of eisosomes; associated with endocytosis, along with Pil1p and Lsp1p; sporulation and plasma membrane sphingolipid content are altered in mutants Gene:TEP1(YNL128W)|FD-Score:3.3|P-value:4.75E-4||SGD DESC:PTEN homolog with no demonstrated inositol lipid phosphatase activity; plays a role in normal sporulation; homolog of human tumor suppressor gene PTEN/MMAC1/TEP1 and fission yeast ptn1 Gene:THI20(YOL055C)|FD-Score:-6.04|P-value:7.74E-10||SGD DESC:Trifunctional enzyme of thiamine biosynthesis, degradation and salvage; has hydroxymethylpyrimidine (HMP) kinase, HMP-phosphate (HMP-P) kinase and thiaminase activities; member of a gene family with THI21 and THI22; HMP and HMP-P kinase activity redundant with Thi21p Gene:THR4(YCR053W)|FD-Score:-3.27|P-value:5.38E-4||SGD DESC:Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:VAM10(YOR068C)|FD-Score:-3.87|P-value:5.42E-5||SGD DESC:Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering Gene:VBA4(YDR119W_p)|FD-Score:-3.13|P-value:8.80E-4||SGD DESC:Protein of unknown function with proposed role as a basic amino acid permease based on phylogeny; GFP-fusion protein localizes to vacuolar membrane; physical interaction with Atg27p suggests a possible role in autophagy; non-essential gene Gene:VPS13(YLL040C)|FD-Score:3.96|P-value:3.81E-5||SGD DESC:Protein of unknown function; heterooligomeric or homooligomeric complex; peripherally associated with membranes; involved in sporulation, vacuolar protein sorting, prospore membrane formation and protein-Golgi retention; homologous to human CHAC and COH1 which are involved in chorea acanthocytosis and Cohen syndrome, respectively Gene:VPS25(YJR102C)|FD-Score:-3.43|P-value:3.07E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS3(YDR495C)|FD-Score:3.22|P-value:6.37E-4||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS75(YNL246W)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress Gene:WSC2(YNL283C)|FD-Score:4.45|P-value:4.37E-6||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response Gene:YBR238C(YBR238C)|FD-Score:4.02|P-value:2.89E-5||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YBR242W(YBR242W_p)|FD-Score:3.13|P-value:8.60E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene Gene:YDL041W(YDL041W_d)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YDR003W-A(YDR003W-A_p)|FD-Score:4.19|P-value:1.38E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YDR008C(YDR008C_d)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YKE2(YLR200W)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin Gene:YNR065C(YNR065C_p)|FD-Score:3.83|P-value:6.54E-5||SGD DESC:Protein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene Gene:YOR385W(YOR385W_p)|FD-Score:-4.26|P-value:1.02E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene Gene:YPL073C(YPL073C_d)|FD-Score:-3.6|P-value:1.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with verified gene UBP16/YPL072W; may interact with ribosome based on co-purification experiments Gene:YPL080C(YPL080C_d)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL182C(YPL182C_d)|FD-Score:3.16|P-value:7.84E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CTI6/YPL181W Gene:YPQ1(YOL092W_p)|FD-Score:3.19|P-value:7.18E-4||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter Gene:YPR011C(YPR011C_p)|FD-Score:-3.33|P-value:4.29E-4||SGD DESC:Putative transporter, member of the mitochondrial carrier family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPS3(YLR121C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:AVL9(YLR114C)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:BSC1(YDL037C)|FD-Score:3.09|P-value:9.86E-4||SGD DESC:Protein of unconfirmed function, similar to cell surface flocculin Flo11p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression Gene:CBT1(YKL208W)|FD-Score:-3.51|P-value:2.22E-4||SGD DESC:Protein involved in 5' end processing of mitochondrial COB, 15S_rRNA, and RPM1 transcripts; may also have a role in 3' end processing of the COB pre-mRNA; displays genetic interaction with cell cycle-regulated kinase Dbf2p Gene:COS1(YNL336W)|FD-Score:4.87|P-value:5.45E-7||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:CSM3(YMR048W)|FD-Score:-3.71|P-value:1.04E-4||SGD DESC:Replication fork associated factor; required for stable replication fork pausing; component of the DNA replication checkpoint pathway; required for accurate chromosome segregation during meiosis; forms nuclear foci upon DNA replication stress Gene:CYB5(YNL111C)|FD-Score:3.97|P-value:3.62E-5||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:DAS1(YJL149W)|FD-Score:3.89|P-value:5.06E-5||SGD DESC:Putative SCF ubiquitin ligase F-box protein; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C Gene:DEG1(YFL001W)|FD-Score:4.25|P-value:1.06E-5||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability Gene:DYN2(YDR424C)|FD-Score:3.84|P-value:6.26E-5||SGD DESC:Cytoplasmic light chain dynein, microtubule motor protein; proposed to be involved in the assembly of the nuclear pore complex Gene:EFT1(YOR133W)|FD-Score:-4.1|P-value:2.06E-5||SGD DESC:Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication Gene:FRD1(YEL047C)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Soluble fumarate reductase; required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to Arxula adeninovorans fumarate reductase; protein abundance increases in response to DNA replication stress; FRD1 has a paralog, OSM1, that arose from the whole genome duplication Gene:FYV4(YHR059W)|FD-Score:4.2|P-value:1.33E-5||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GET3(YDL100C)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GUT1(YHL032C)|FD-Score:3.76|P-value:8.38E-5||SGD DESC:Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p Gene:HXT1(YHR094C)|FD-Score:4.33|P-value:7.53E-6||SGD DESC:Low-affinity glucose transporter of the major facilitator superfamily, expression is induced by Hxk2p in the presence of glucose and repressed by Rgt1p when glucose is limiting; HXT1 has a paralog, HXT6, what arose from the whole genome duplication Gene:IFA38(YBR159W)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides Gene:IRC21(YMR073C)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:JLP2(YMR132C_p)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:Protein of unknown function, contains sequence that closely resembles a J domain (typified by the E. coli DnaJ protein) Gene:KCS1(YDR017C)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; generation of high energy inositol pyrophosphates by Kcs1p is required for many processes such as vacuolar biogenesis, stress response and telomere maintenance Gene:LAS21(YJL062W)|FD-Score:3.15|P-value:8.20E-4||SGD DESC:Integral plasma membrane protein involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity Gene:LSM1(YJL124C)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:MEU1(YLR017W)|FD-Score:3.12|P-value:9.15E-4||SGD DESC:Methylthioadenosine phosphorylase (MTAP), catalyzes the initial step in the methionine salvage pathway; affects polyamine biosynthesis through regulation of ornithine decarboxylase (Spe1p) activity; regulates ADH2 gene expression Gene:MID2(YLR332W)|FD-Score:3.37|P-value:3.82E-4||SGD DESC:O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p Gene:MKK2(YPL140C)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk1p; MKK2 has a paralog, MKK1, that arose from the whole genome duplication Gene:MSC6(YOR354C)|FD-Score:3.28|P-value:5.28E-4||SGD DESC:Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:MTC7(YEL033W_p)|FD-Score:4.31|P-value:8.33E-6||SGD DESC:Predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant Gene:PAN3(YKL025C)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PNG1(YPL096W)|FD-Score:3.2|P-value:6.83E-4||SGD DESC:Conserved peptide N-glycanase required for deglycosylation of misfolded glycoproteins during proteasome-dependent degradation; localizes to the cytoplasm and nucleus; activity is enhanced by interaction with Rad23p Gene:PPZ2(YDR436W)|FD-Score:-3.23|P-value:6.09E-4||SGD DESC:Serine/threonine protein phosphatase Z, isoform of Ppz1p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance Gene:PRS3(YHL011C)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:PUF4(YGL014W)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors Gene:RFM1(YOR279C)|FD-Score:-3.11|P-value:9.46E-4||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RGS2(YOR107W)|FD-Score:3.15|P-value:8.04E-4||SGD DESC:Negative regulator of glucose-induced cAMP signaling; directly activates the GTPase activity of the heterotrimeric G protein alpha subunit Gpa2p Gene:RLM1(YPL089C)|FD-Score:4.13|P-value:1.83E-5||SGD DESC:MADS-box transcription factor; component of the protein kinase C-mediated MAP kinase pathway involved in the maintenance of cell integrity; phosphorylated and activated by the MAP-kinase Slt2p; RLM1 has a paralog, SMP1, that arose from the whole genome duplication Gene:RTN2(YDL204W)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; much less abundant than Rtn1p; rtn1 rtn2 yop1 triple mutant lacks tubular ER; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress; RTN2 has a paralog, RTN1, that arose from the whole genome duplication Gene:SAM4(YPL273W)|FD-Score:-3.24|P-value:6.00E-4||SGD DESC:S-adenosylmethionine-homocysteine methyltransferase, functions along with Mht1p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio Gene:SLM2(YNL047C)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm1p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; SLM2 has a paralog, SLM1, that arose from the whole genome duplication Gene:SMA1(YPL027W)|FD-Score:-3.19|P-value:7.01E-4||SGD DESC:Protein of unknown function involved in the assembly of the prospore membrane during sporulation; interacts with Spo14p Gene:SNL1(YIL016W)|FD-Score:-3.13|P-value:8.71E-4||SGD DESC:Ribosome-associated protein proposed to act in protein synthesis and nuclear pore complex biogenesis and maintenance as well as protein folding; has similarity to the mammalian BAG-1 protein Gene:SUR7(YML052W)|FD-Score:-4.39|P-value:5.54E-6||SGD DESC:Plasma membrane protein that localizes to furrow-like invaginations (MCC patches); component of eisosomes; associated with endocytosis, along with Pil1p and Lsp1p; sporulation and plasma membrane sphingolipid content are altered in mutants Gene:TEP1(YNL128W)|FD-Score:3.3|P-value:4.75E-4||SGD DESC:PTEN homolog with no demonstrated inositol lipid phosphatase activity; plays a role in normal sporulation; homolog of human tumor suppressor gene PTEN/MMAC1/TEP1 and fission yeast ptn1 Gene:THI20(YOL055C)|FD-Score:-6.04|P-value:7.74E-10||SGD DESC:Trifunctional enzyme of thiamine biosynthesis, degradation and salvage; has hydroxymethylpyrimidine (HMP) kinase, HMP-phosphate (HMP-P) kinase and thiaminase activities; member of a gene family with THI21 and THI22; HMP and HMP-P kinase activity redundant with Thi21p Gene:THR4(YCR053W)|FD-Score:-3.27|P-value:5.38E-4||SGD DESC:Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:VAM10(YOR068C)|FD-Score:-3.87|P-value:5.42E-5||SGD DESC:Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering Gene:VBA4(YDR119W_p)|FD-Score:-3.13|P-value:8.80E-4||SGD DESC:Protein of unknown function with proposed role as a basic amino acid permease based on phylogeny; GFP-fusion protein localizes to vacuolar membrane; physical interaction with Atg27p suggests a possible role in autophagy; non-essential gene Gene:VPS13(YLL040C)|FD-Score:3.96|P-value:3.81E-5||SGD DESC:Protein of unknown function; heterooligomeric or homooligomeric complex; peripherally associated with membranes; involved in sporulation, vacuolar protein sorting, prospore membrane formation and protein-Golgi retention; homologous to human CHAC and COH1 which are involved in chorea acanthocytosis and Cohen syndrome, respectively Gene:VPS25(YJR102C)|FD-Score:-3.43|P-value:3.07E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS3(YDR495C)|FD-Score:3.22|P-value:6.37E-4||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS75(YNL246W)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress Gene:WSC2(YNL283C)|FD-Score:4.45|P-value:4.37E-6||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response Gene:YBR238C(YBR238C)|FD-Score:4.02|P-value:2.89E-5||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YBR242W(YBR242W_p)|FD-Score:3.13|P-value:8.60E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene Gene:YDL041W(YDL041W_d)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YDR003W-A(YDR003W-A_p)|FD-Score:4.19|P-value:1.38E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YDR008C(YDR008C_d)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YKE2(YLR200W)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin Gene:YNR065C(YNR065C_p)|FD-Score:3.83|P-value:6.54E-5||SGD DESC:Protein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene Gene:YOR385W(YOR385W_p)|FD-Score:-4.26|P-value:1.02E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene Gene:YPL073C(YPL073C_d)|FD-Score:-3.6|P-value:1.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with verified gene UBP16/YPL072W; may interact with ribosome based on co-purification experiments Gene:YPL080C(YPL080C_d)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL182C(YPL182C_d)|FD-Score:3.16|P-value:7.84E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CTI6/YPL181W Gene:YPQ1(YOL092W_p)|FD-Score:3.19|P-value:7.18E-4||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter Gene:YPR011C(YPR011C_p)|FD-Score:-3.33|P-value:4.29E-4||SGD DESC:Putative transporter, member of the mitochondrial carrier family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPS3(YLR121C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YER003C4.817.50E-70.52PMI40Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation
YHR083W4.299.03E-60.45SAM35Essential component of the sorting and assembly machinery (SAM complex or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane
YDR212W3.846.14E-50.34TCP1Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein
YMR033W3.502.33E-40.14ARP9Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YPL122C3.363.84E-40.04TFB2Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH
YFR005C3.334.40E-40.00SAD1Conserved zinc-finger domain protein involved in pre-mRNA splicing, required for assembly of U4 snRNA into the U4/U6 particle
YIL126W3.324.47E-40.12STH1ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p
YBR136W3.206.95E-40.03MEC1Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres
YFL045C3.167.80E-40.03SEC53Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen
YOR319W3.138.68E-40.04HSH49U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM)
YOR217W3.099.99E-40.17RFC1Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
YMR220W2.920.001740.02ERG8Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YDR240C2.900.001880.07SNU56Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex
YGR060W2.830.002350.02ERG25C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol
YPL252C2.800.002530.04YAH1Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YNL336W4.875.45E-7COS1Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins
YNL283C4.454.37E-6WSC2Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response
YHR094C4.337.53E-6HXT1Low-affinity glucose transporter of the major facilitator superfamily, expression is induced by Hxk2p in the presence of glucose and repressed by Rgt1p when glucose is limiting; HXT1 has a paralog, HXT6, what arose from the whole genome duplication
YEL033W_p4.318.33E-6MTC7_pPredicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant
YFL001W4.251.06E-5DEG1tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability
YHR059W4.201.33E-5FYV4Protein of unknown function, required for survival upon exposure to K1 killer toxin
YDR003W-A_p4.191.38E-5YDR003W-A_pPutative protein of unknown function; identified by expression profiling and mass spectrometry
YPL089C4.131.83E-5RLM1MADS-box transcription factor; component of the protein kinase C-mediated MAP kinase pathway involved in the maintenance of cell integrity; phosphorylated and activated by the MAP-kinase Slt2p; RLM1 has a paralog, SMP1, that arose from the whole genome duplication
YBR238C4.022.89E-5YBR238CMitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span
YNL111C3.973.62E-5CYB5Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation
YLL040C3.963.81E-5VPS13Protein of unknown function; heterooligomeric or homooligomeric complex; peripherally associated with membranes; involved in sporulation, vacuolar protein sorting, prospore membrane formation and protein-Golgi retention; homologous to human CHAC and COH1 which are involved in chorea acanthocytosis and Cohen syndrome, respectively
YJL149W3.895.06E-5DAS1Putative SCF ubiquitin ligase F-box protein; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C
YDR424C3.846.26E-5DYN2Cytoplasmic light chain dynein, microtubule motor protein; proposed to be involved in the assembly of the nuclear pore complex
YNR065C_p3.836.54E-5YNR065C_pProtein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene
YHL032C3.768.38E-5GUT1Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p

GO enrichment analysis for SGTC_2706
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0707.02E-8SGTC_412gemfibrozil 100.0 μMMiscellaneous34630.09375DNA intercalators
0.0631.23E-6SGTC_31409094243 49.5 μMChembridge (Drug-like library)192911120.19697
0.0621.73E-6SGTC_21485546263 113.6 μMChembridge (Fragment library)7920970.0483871
0.0587.10E-6SGTC_1463448-8650 53.1 μMChemDiv (Drug-like library)28914880.0886076NEO1-PIK1
0.0589.62E-6SGTC_74000s-0487 160.3 μMChemDiv (Drug-like library)6775480.131148
0.0571.47E-5SGTC_2662daunorubicin 22.4 μMMiscellaneous303230.119565anthracycline transcription coupled DNA repair
0.0571.36E-5SGTC_414piperonyl butoxide 100.0 μMMiscellaneous57940.0461538
0.0543.20E-5SGTC_1875nocodazole 6.0 μMMiscellaneous41220.0958904tubulin folding & SWR complex
0.0534.03E-5SGTC_13913442-0225 151.0 μMChemDiv (Drug-like library)52270750.166667
0.0534.18E-5SGTC_32759138069 49.5 μMChembridge (Drug-like library)176170070.1
0.0535.34E-5SGTC_331k048-0133 24.5 μMChemDiv (Drug-like library)68135820.0606061RSC & ERG11
0.0517.82E-5SGTC_342k048-0088 23.3 μMChemDiv (Drug-like library)68205700.0869565RSC & ERG11
0.0519.16E-5SGTC_27875284540 71.4 μMChembridge (Drug-like library)7369580.111111
0.0519.23E-5SGTC_1731st035964 63.0 μMTimTec (Natural product derivative library)67395750.0869565
0.0519.26E-5SGTC_1493970-0822 46.1 μMChemDiv (Drug-like library)31142500.0821918tubulin folding & SWR complex

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2715st07950056.4 μM0.32258128246688TimTec (Natural product derivative library)354.422822.92314
SGTC_22957971645200 μM0.31252978226Chembridge (Fragment library)204.268242.09911
SGTC_3096911746849.47 μM0.29090941316311Chembridge (Drug-like library)247.316121.1614
SGTC_21785812956200 μM0.2830195403383Chembridge (Fragment library)215.251121.49212
SGTC_900870-08634.61 μM0.2758621738164ChemDiv (Drug-like library)311.762263.53812
SGTC_1990st07205059.8 μM0.27586217233948TimTec (Natural product derivative library)334.433182.70514
SGTC_3043909230349.47 μM0.27272725236084Chembridge (Drug-like library)299.430443.97302
SGTC_14214029-024971.4 μM0.2678572894095ChemDiv (Drug-like library)283.3400233.71802
SGTC_2840900877171.43 μM0.2656252992978Chembridge (Drug-like library)364.437563.54613
SGTC_2214717929748.28 μM0.265306769744Chembridge (Fragment library)235.302121.27603superoxide