rotenone

Inhibits electron transfer from iron-sulfur centers in mitochondria.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_271
Screen concentration 740.0 μM
Source Miscellaneous
PubChem CID 5102
SMILES CC(=C)C1CC2=C(O1)C=CC3=C2OC4COC5=CC(=C(C=C5C4C3=O)OC)OC
Standardized SMILES COc1cc2OCC3Oc4c5CC(Oc5ccc4C(=O)C3c2cc1OC)C(=C)C
Molecular weight 394.4172
ALogP 3.93
H-bond donor count 0
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.12
% growth inhibition (Hom. pool) 4.56


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5102
Download HIP data (tab-delimited text)  (excel)
Gene:APC1(YNL172W)|FD-Score:4.12|P-value:1.87E-5|Clearance:0.72||SGD DESC:Largest subunit of the Anaphase-Promoting Complex/Cyclosome; APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:COG3(YER157W)|FD-Score:-4.71|P-value:1.24E-6|Clearance:0||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:FBA1(YKL060C)|FD-Score:3.11|P-value:9.47E-4|Clearance:0.02||SGD DESC:Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversion of fructose 1,6 bisphosphate to glyceraldehyde-3-P and dihydroxyacetone-P; locates to mitochondrial outer surface upon oxidative stress Gene:GAB1(YLR459W)|FD-Score:3.83|P-value:6.38E-5|Clearance:0.72||SGD DESC:GPI transamidase subunit, involved in attachment of glycosylphosphatidylinositol (GPI) anchors to proteins; may have a role in recognition of the attachment signal or of the lipid portion of GPI Gene:YGR114C(YGR114C_d)|FD-Score:4.04|P-value:2.66E-5|Clearance:0.72||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:APC1(YNL172W)|FD-Score:4.12|P-value:1.87E-5|Clearance:0.72||SGD DESC:Largest subunit of the Anaphase-Promoting Complex/Cyclosome; APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:COG3(YER157W)|FD-Score:-4.71|P-value:1.24E-6|Clearance:0||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:FBA1(YKL060C)|FD-Score:3.11|P-value:9.47E-4|Clearance:0.02||SGD DESC:Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversion of fructose 1,6 bisphosphate to glyceraldehyde-3-P and dihydroxyacetone-P; locates to mitochondrial outer surface upon oxidative stress Gene:GAB1(YLR459W)|FD-Score:3.83|P-value:6.38E-5|Clearance:0.72||SGD DESC:GPI transamidase subunit, involved in attachment of glycosylphosphatidylinositol (GPI) anchors to proteins; may have a role in recognition of the attachment signal or of the lipid portion of GPI Gene:YGR114C(YGR114C_d)|FD-Score:4.04|P-value:2.66E-5|Clearance:0.72||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5102
Download HOP data (tab-delimited text)  (excel)
Gene:AAP1(YHR047C)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:Arginine/alanine amino peptidase; overproduction stimulates glycogen accumulation; AAP1 has a paralog, APE2, that arose from the whole genome duplication Gene:ANR2(YKL047W_p)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Putative protein of unknown function, predicted to be palmitoylated; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:APE3(YBR286W)|FD-Score:4.7|P-value:1.32E-6||SGD DESC:Vacuolar aminopeptidase Y, processed to mature form by Prb1p Gene:ATG7(YHR171W)|FD-Score:-3.72|P-value:1.00E-4||SGD DESC:Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation Gene:ATO3(YDR384C)|FD-Score:5.48|P-value:2.10E-8||SGD DESC:Plasma membrane protein, regulation pattern suggests a possible role in export of ammonia from the cell; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family of putative transporters Gene:AVT5(YBL089W)|FD-Score:6.17|P-value:3.35E-10||SGD DESC:Putative transporter; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters; AVT5 has a paralog, AVT6, that arose from the whole genome duplication Gene:AYR1(YIL124W)|FD-Score:4.66|P-value:1.56E-6||SGD DESC:NADPH-dependent 1-acyl dihydroxyacetone phosphate reductase found in lipid particles, ER, and mitochondrial outer membrane; involved in phosphatidic acid biosynthesis; required for spore germination; capable of metabolizing steroid hormones Gene:BNI4(YNL233W)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p Gene:BTS1(YPL069C)|FD-Score:-3.53|P-value:2.05E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CKA2(YOR061W)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching Gene:COG6(YNL041C)|FD-Score:8.42|P-value:1.83E-17||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:5.83|P-value:2.76E-9||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DAT1(YML113W)|FD-Score:-3.22|P-value:6.47E-4||SGD DESC:DNA binding protein that recognizes oligo(dA).oligo(dT) tracts; Arg side chain in its N-terminal pentad Gly-Arg-Lys-Pro-Gly repeat is required for DNA-binding; not essential for viability Gene:DGK1(YOR311C)|FD-Score:3.12|P-value:8.93E-4||SGD DESC:Diacylglycerol kinase, localized to the endoplasmic reticulum (ER); overproduction induces enlargement of ER-like membrane structures and suppresses a temperature-sensitive sly1 mutation; contains a CTP transferase domain Gene:DTD1(YDL219W)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:D-Tyr-tRNA(Tyr) deacylase, functions in protein translation, may affect nonsense suppression via alteration of the protein synthesis machinery; ubiquitous among eukaryotes Gene:ELG1(YOR144C)|FD-Score:3.31|P-value:4.63E-4||SGD DESC:Subunit of an alternative replication factor C complex important for DNA replication and genome integrity; suppresses spontaneous DNA damage; involved in homologous recombination-mediated repair and telomere homeostasis; required for PCNA (Pol30p) unloading during DNA replication Gene:EMI5(YOL071W)|FD-Score:4.43|P-value:4.70E-6||SGD DESC:Subunit of succinate dehydrogenase, which couples succinate oxidation to ubiquinone reduction; required for FAD cofactor attachment to Sdh1p; mutations in human ortholog PGL2 are associated with neuroendocrine tumors (paraganglioma) Gene:FLO11(YIR019C)|FD-Score:3.98|P-value:3.43E-5||SGD DESC:GPI-anchored cell surface glycoprotein (flocculin); required for pseudohyphal formation, invasive growth, flocculation, and biofilms; transcriptionally regulated by the MAPK pathway (via Ste12p and Tec1p) and the cAMP pathway (via Flo8p); required for the formation of fibrous interconnections between cells in a colony of a wild S. cerevisiae strain Gene:FMP48(YGR052W_p)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FUR4(YBR021W)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GCN3(YKR026C)|FD-Score:3.13|P-value:8.79E-4||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GPG1(YGL121C)|FD-Score:3.19|P-value:7.19E-4||SGD DESC:Proposed gamma subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p; involved in regulation of pseudohyphal growth; requires Gpb1p or Gpb2p to interact with Gpa2p; overproduction causes prion curing Gene:HSP26(YBR072W)|FD-Score:4.86|P-value:5.79E-7||SGD DESC:Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; oligomer activation requires heat-induced conformational change; also has mRNA binding activity Gene:LAG2(YOL025W)|FD-Score:-3.19|P-value:7.00E-4||SGD DESC:Protein that negatively regulates the SCF E3-ubiquitin ligase by interacting with and preventing neddyation of the cullin subunit, Cdc53p; longevity determinant that is preferentially expressed in young cells; similar to mammalian Cand1 Gene:LOH1(YJL038C)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Protein of unknown function with proposed roles in maintenance of genome integrity and also in spore wall assembly; induced during sporulation; repressed during vegetative growth by Sum1p and Hst1p; sequence similar to IRC1 Gene:MAK3(YPR051W)|FD-Score:4.01|P-value:3.07E-5||SGD DESC:Catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus Gene:MAP1(YLR244C)|FD-Score:3.15|P-value:8.24E-4||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p Gene:MCM16(YPR046W)|FD-Score:3.12|P-value:9.16E-4||SGD DESC:Component of the Ctf19 complex and the COMA subcomplex; involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-H and fission yeast fta3 Gene:MEU1(YLR017W)|FD-Score:4.05|P-value:2.61E-5||SGD DESC:Methylthioadenosine phosphorylase (MTAP), catalyzes the initial step in the methionine salvage pathway; affects polyamine biosynthesis through regulation of ornithine decarboxylase (Spe1p) activity; regulates ADH2 gene expression Gene:MRI1(YPR118W)|FD-Score:-3.16|P-value:8.00E-4||SGD DESC:5'-methylthioribose-1-phosphate isomerase; catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway Gene:NHX1(YDR456W)|FD-Score:3.86|P-value:5.71E-5||SGD DESC:Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion Gene:NUP84(YDL116W)|FD-Score:3.38|P-value:3.63E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP107 Gene:PCK1(YKR097W)|FD-Score:5.2|P-value:9.87E-8||SGD DESC:Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol Gene:PCL1(YNL289W)|FD-Score:5.37|P-value:3.92E-8||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; is ubiquitinated by Dma1p; phosphorylation by Pho85p targets it for degradation; localizes to sites of polarized cell growth Gene:PEX13(YLR191W)|FD-Score:4.59|P-value:2.26E-6||SGD DESC:Integral peroxisomal membrane protein required for translocation of peroxisomal matrix proteins, interacts with the PTS1 signal recognition factor Pex5p and the PTS2 signal recognition factor Pex7p, forms a complex with Pex14p and Pex17p Gene:PIP2(YOR363C)|FD-Score:3.29|P-value:4.97E-4||SGD DESC:Autoregulatory oleate-specific transcriptional activator of peroxisome proliferation, contains Zn(2)-Cys(6) cluster domain, forms heterodimer with Oaf1p, binds oleate response elements (OREs), activates beta-oxidation genes; PIP2 has a paralog, OAF1, that arose from the whole genome duplication Gene:PMS1(YNL082W)|FD-Score:4.76|P-value:9.65E-7||SGD DESC:ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL Gene:PNG1(YPL096W)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Conserved peptide N-glycanase required for deglycosylation of misfolded glycoproteins during proteasome-dependent degradation; localizes to the cytoplasm and nucleus; activity is enhanced by interaction with Rad23p Gene:PPT2(YPL148C)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PRM5(YIL117C)|FD-Score:5.78|P-value:3.68E-9||SGD DESC:Pheromone-regulated protein, predicted to have 1 transmembrane segment; induced during cell integrity signaling Gene:PRS1(YKL181W)|FD-Score:3.77|P-value:8.23E-5||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:RAD28(YDR030C)|FD-Score:4.33|P-value:7.60E-6||SGD DESC:Protein involved in DNA repair, related to the human CSA protein that is involved in transcription-coupled repair nucleotide excision repair Gene:RBK1(YCR036W)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Putative ribokinase Gene:REX2(YLR059C)|FD-Score:3.18|P-value:7.26E-4||SGD DESC:3'-5' RNA exonuclease; involved in 3'-end processing of U4 and U5 snRNAs, 5S and 5.8S rRNAs, and RNase P and RNase MRP RNA; localized to mitochondria and null suppresses escape of mtDNA to nucleus in yme1 yme2 mutants; RNase D exonuclease Gene:RIM4(YHL024W)|FD-Score:5.37|P-value:3.91E-8||SGD DESC:Putative RNA-binding protein required for the expression of early and middle sporulation genes Gene:RPH1(YER169W)|FD-Score:-3.38|P-value:3.68E-4||SGD DESC:JmjC domain-containing histone demethylase; specifically demethylates H3K36 tri- and dimethyl modification states; associates with actively transcribed (RNA polymerase II) regions in vivo and specifically targets H3K36 in its trimethylation state as its substrate; transcriptional repressor of PHR1; Rph1p phosphorylation during DNA damage is under control of the MEC1-RAD53 pathway; RPH1 has a paralog, GIS1, that arose from the whole genome duplication Gene:SCT1(YBL011W)|FD-Score:3.78|P-value:7.81E-5||SGD DESC:Glycerol 3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase of the glycerolipid biosynthesis pathway, prefers 16-carbon fatty acids, similar to Gpt2p, gene is constitutively transcribed Gene:SLF1(YDR515W)|FD-Score:-3.25|P-value:5.76E-4||SGD DESC:RNA binding protein that associates with polysomes; may be involved in regulating mRNA translation; involved in the copper-dependent mineralization of copper sulfide complexes on cell surface in cells cultured in copper salts; SLF1 has a paralog, SRO9, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SPR28(YDR218C)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Sporulation-specific homolog of the yeast CDC3/10/11/12 family of bud neck microfilament genes; meiotic septin expressed at high levels during meiotic divisions and ascospore formation Gene:SWR1(YDR334W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:TMA23(YMR269W)|FD-Score:3.82|P-value:6.55E-5||SGD DESC:Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan Gene:TPM2(YIL138C)|FD-Score:3.76|P-value:8.52E-5||SGD DESC:Minor isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; appears to have distinct and also overlapping functions with Tpm1p; TPM2 has a paralog, TPM1, that arose from the whole genome duplication Gene:TRM82(YDR165W)|FD-Score:3.25|P-value:5.77E-4||SGD DESC:Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p Gene:TRP4(YDR354W)|FD-Score:3.21|P-value:6.69E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TUL1(YKL034W)|FD-Score:3.13|P-value:8.68E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1 Gene:TUM1(YOR251C)|FD-Score:3.92|P-value:4.52E-5||SGD DESC:Rhodanese domain sulfur transferase, accepts persulfite from Nfs1p and transfers it to Uba4p in the pathway for 2-thiolation of the wobble uridine base of tRNAs; also stimulates sulfur transfer by Nfs1p; may be mitochondrially localized Gene:UBI4(YLL039C)|FD-Score:-4.43|P-value:4.67E-6||SGD DESC:Ubiquitin; becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-26S proteasome system; essential for the cellular stress response; encoded as a polyubiquitin precursor comprised of 5 head-to-tail repeats; protein abundance increases in response to DNA replication stress Gene:UTP30(YKR060W)|FD-Score:-3.12|P-value:8.91E-4||SGD DESC:Putative subunit of U3-containing 90S preribosome complex; complex is involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:VPS4(YPR173C)|FD-Score:3.93|P-value:4.29E-5||SGD DESC:AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism Gene:VPS71(YML041C)|FD-Score:-3.66|P-value:1.25E-4||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YAR023C(YAR023C_p)|FD-Score:-3.52|P-value:2.16E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family Gene:YBR113W(YBR113W_d)|FD-Score:4.64|P-value:1.71E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CYC8 Gene:YBR206W(YBR206W_d)|FD-Score:3.96|P-value:3.74E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KTR3 Gene:YCR007C(YCR007C_p)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene Gene:YDR215C(YDR215C_d)|FD-Score:-3.3|P-value:4.77E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein Gene:YFL012W(YFL012W_p)|FD-Score:3.19|P-value:7.03E-4||SGD DESC:Putative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin Gene:YFL019C(YFL019C_d)|FD-Score:-3.09|P-value:9.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene Gene:YGR125W(YGR125W_p)|FD-Score:3.88|P-value:5.27E-5||SGD DESC:Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YHK8(YHR048W_p)|FD-Score:-3.35|P-value:4.10E-4||SGD DESC:Presumed antiporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; expression of gene is up-regulated in cells exhibiting reduced susceptibility to azoles Gene:YIG1(YPL201C)|FD-Score:3.75|P-value:8.79E-5||SGD DESC:Protein that interacts with glycerol 3-phosphatase and plays a role in anaerobic glycerol production; localizes to the nucleus and cytosol Gene:YJR003C(YJR003C_p)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; predicted to be involved in ribosome biogenesis Gene:YKL118W(YKL118W_d)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YLR342W-A(YLR342W-A_p)|FD-Score:3.75|P-value:8.96E-5||SGD DESC:Putative protein of unknown function Gene:YML007C-A(YML007C-A_p)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YML020W(YML020W_p)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Putative protein of unknown function Gene:YMR001C-A(YMR001C-A_p)|FD-Score:4.2|P-value:1.31E-5||SGD DESC:Putative protein of unknown function Gene:YNL095C(YNL095C_p)|FD-Score:-4.66|P-value:1.59E-6||SGD DESC:Putative protein of unknown function; predicted to contain a transmembrane domain; not an essential gene; YNL095C has a paralog, ECM3, that arose from the whole genome duplication Gene:YNL338W(YNL338W_d)|FD-Score:3.18|P-value:7.44E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps TEL14L-XC, which is Telomeric X element Core sequence on the left arm of Chromosome XIV Gene:YNR064C(YNR064C)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Epoxide hydrolase, member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides Gene:YOR055W(YOR055W_d)|FD-Score:3.26|P-value:5.55E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPK9(YOR291W)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-type ATPase Spf1p; homolog of human ATP13A2 (PARK9), mutations in which are associated with Parkinson disease and Kufor-Rakeb syndrome Gene:YSR3(YKR053C)|FD-Score:4.01|P-value:3.09E-5||SGD DESC:Dihydrosphingosine 1-phosphate phosphatase; membrane protein involved in sphingolipid metabolism; YSR3 has a paralog, LCB3, that arose from the whole genome duplication Gene:AAP1(YHR047C)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:Arginine/alanine amino peptidase; overproduction stimulates glycogen accumulation; AAP1 has a paralog, APE2, that arose from the whole genome duplication Gene:ANR2(YKL047W_p)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Putative protein of unknown function, predicted to be palmitoylated; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:APE3(YBR286W)|FD-Score:4.7|P-value:1.32E-6||SGD DESC:Vacuolar aminopeptidase Y, processed to mature form by Prb1p Gene:ATG7(YHR171W)|FD-Score:-3.72|P-value:1.00E-4||SGD DESC:Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation Gene:ATO3(YDR384C)|FD-Score:5.48|P-value:2.10E-8||SGD DESC:Plasma membrane protein, regulation pattern suggests a possible role in export of ammonia from the cell; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family of putative transporters Gene:AVT5(YBL089W)|FD-Score:6.17|P-value:3.35E-10||SGD DESC:Putative transporter; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters; AVT5 has a paralog, AVT6, that arose from the whole genome duplication Gene:AYR1(YIL124W)|FD-Score:4.66|P-value:1.56E-6||SGD DESC:NADPH-dependent 1-acyl dihydroxyacetone phosphate reductase found in lipid particles, ER, and mitochondrial outer membrane; involved in phosphatidic acid biosynthesis; required for spore germination; capable of metabolizing steroid hormones Gene:BNI4(YNL233W)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p Gene:BTS1(YPL069C)|FD-Score:-3.53|P-value:2.05E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CKA2(YOR061W)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching Gene:COG6(YNL041C)|FD-Score:8.42|P-value:1.83E-17||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:5.83|P-value:2.76E-9||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DAT1(YML113W)|FD-Score:-3.22|P-value:6.47E-4||SGD DESC:DNA binding protein that recognizes oligo(dA).oligo(dT) tracts; Arg side chain in its N-terminal pentad Gly-Arg-Lys-Pro-Gly repeat is required for DNA-binding; not essential for viability Gene:DGK1(YOR311C)|FD-Score:3.12|P-value:8.93E-4||SGD DESC:Diacylglycerol kinase, localized to the endoplasmic reticulum (ER); overproduction induces enlargement of ER-like membrane structures and suppresses a temperature-sensitive sly1 mutation; contains a CTP transferase domain Gene:DTD1(YDL219W)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:D-Tyr-tRNA(Tyr) deacylase, functions in protein translation, may affect nonsense suppression via alteration of the protein synthesis machinery; ubiquitous among eukaryotes Gene:ELG1(YOR144C)|FD-Score:3.31|P-value:4.63E-4||SGD DESC:Subunit of an alternative replication factor C complex important for DNA replication and genome integrity; suppresses spontaneous DNA damage; involved in homologous recombination-mediated repair and telomere homeostasis; required for PCNA (Pol30p) unloading during DNA replication Gene:EMI5(YOL071W)|FD-Score:4.43|P-value:4.70E-6||SGD DESC:Subunit of succinate dehydrogenase, which couples succinate oxidation to ubiquinone reduction; required for FAD cofactor attachment to Sdh1p; mutations in human ortholog PGL2 are associated with neuroendocrine tumors (paraganglioma) Gene:FLO11(YIR019C)|FD-Score:3.98|P-value:3.43E-5||SGD DESC:GPI-anchored cell surface glycoprotein (flocculin); required for pseudohyphal formation, invasive growth, flocculation, and biofilms; transcriptionally regulated by the MAPK pathway (via Ste12p and Tec1p) and the cAMP pathway (via Flo8p); required for the formation of fibrous interconnections between cells in a colony of a wild S. cerevisiae strain Gene:FMP48(YGR052W_p)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FUR4(YBR021W)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:GCN3(YKR026C)|FD-Score:3.13|P-value:8.79E-4||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GPG1(YGL121C)|FD-Score:3.19|P-value:7.19E-4||SGD DESC:Proposed gamma subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p; involved in regulation of pseudohyphal growth; requires Gpb1p or Gpb2p to interact with Gpa2p; overproduction causes prion curing Gene:HSP26(YBR072W)|FD-Score:4.86|P-value:5.79E-7||SGD DESC:Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; oligomer activation requires heat-induced conformational change; also has mRNA binding activity Gene:LAG2(YOL025W)|FD-Score:-3.19|P-value:7.00E-4||SGD DESC:Protein that negatively regulates the SCF E3-ubiquitin ligase by interacting with and preventing neddyation of the cullin subunit, Cdc53p; longevity determinant that is preferentially expressed in young cells; similar to mammalian Cand1 Gene:LOH1(YJL038C)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Protein of unknown function with proposed roles in maintenance of genome integrity and also in spore wall assembly; induced during sporulation; repressed during vegetative growth by Sum1p and Hst1p; sequence similar to IRC1 Gene:MAK3(YPR051W)|FD-Score:4.01|P-value:3.07E-5||SGD DESC:Catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus Gene:MAP1(YLR244C)|FD-Score:3.15|P-value:8.24E-4||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p Gene:MCM16(YPR046W)|FD-Score:3.12|P-value:9.16E-4||SGD DESC:Component of the Ctf19 complex and the COMA subcomplex; involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-H and fission yeast fta3 Gene:MEU1(YLR017W)|FD-Score:4.05|P-value:2.61E-5||SGD DESC:Methylthioadenosine phosphorylase (MTAP), catalyzes the initial step in the methionine salvage pathway; affects polyamine biosynthesis through regulation of ornithine decarboxylase (Spe1p) activity; regulates ADH2 gene expression Gene:MRI1(YPR118W)|FD-Score:-3.16|P-value:8.00E-4||SGD DESC:5'-methylthioribose-1-phosphate isomerase; catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway Gene:NHX1(YDR456W)|FD-Score:3.86|P-value:5.71E-5||SGD DESC:Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion Gene:NUP84(YDL116W)|FD-Score:3.38|P-value:3.63E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP107 Gene:PCK1(YKR097W)|FD-Score:5.2|P-value:9.87E-8||SGD DESC:Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol Gene:PCL1(YNL289W)|FD-Score:5.37|P-value:3.92E-8||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; is ubiquitinated by Dma1p; phosphorylation by Pho85p targets it for degradation; localizes to sites of polarized cell growth Gene:PEX13(YLR191W)|FD-Score:4.59|P-value:2.26E-6||SGD DESC:Integral peroxisomal membrane protein required for translocation of peroxisomal matrix proteins, interacts with the PTS1 signal recognition factor Pex5p and the PTS2 signal recognition factor Pex7p, forms a complex with Pex14p and Pex17p Gene:PIP2(YOR363C)|FD-Score:3.29|P-value:4.97E-4||SGD DESC:Autoregulatory oleate-specific transcriptional activator of peroxisome proliferation, contains Zn(2)-Cys(6) cluster domain, forms heterodimer with Oaf1p, binds oleate response elements (OREs), activates beta-oxidation genes; PIP2 has a paralog, OAF1, that arose from the whole genome duplication Gene:PMS1(YNL082W)|FD-Score:4.76|P-value:9.65E-7||SGD DESC:ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL Gene:PNG1(YPL096W)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Conserved peptide N-glycanase required for deglycosylation of misfolded glycoproteins during proteasome-dependent degradation; localizes to the cytoplasm and nucleus; activity is enhanced by interaction with Rad23p Gene:PPT2(YPL148C)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PRM5(YIL117C)|FD-Score:5.78|P-value:3.68E-9||SGD DESC:Pheromone-regulated protein, predicted to have 1 transmembrane segment; induced during cell integrity signaling Gene:PRS1(YKL181W)|FD-Score:3.77|P-value:8.23E-5||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:RAD28(YDR030C)|FD-Score:4.33|P-value:7.60E-6||SGD DESC:Protein involved in DNA repair, related to the human CSA protein that is involved in transcription-coupled repair nucleotide excision repair Gene:RBK1(YCR036W)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Putative ribokinase Gene:REX2(YLR059C)|FD-Score:3.18|P-value:7.26E-4||SGD DESC:3'-5' RNA exonuclease; involved in 3'-end processing of U4 and U5 snRNAs, 5S and 5.8S rRNAs, and RNase P and RNase MRP RNA; localized to mitochondria and null suppresses escape of mtDNA to nucleus in yme1 yme2 mutants; RNase D exonuclease Gene:RIM4(YHL024W)|FD-Score:5.37|P-value:3.91E-8||SGD DESC:Putative RNA-binding protein required for the expression of early and middle sporulation genes Gene:RPH1(YER169W)|FD-Score:-3.38|P-value:3.68E-4||SGD DESC:JmjC domain-containing histone demethylase; specifically demethylates H3K36 tri- and dimethyl modification states; associates with actively transcribed (RNA polymerase II) regions in vivo and specifically targets H3K36 in its trimethylation state as its substrate; transcriptional repressor of PHR1; Rph1p phosphorylation during DNA damage is under control of the MEC1-RAD53 pathway; RPH1 has a paralog, GIS1, that arose from the whole genome duplication Gene:SCT1(YBL011W)|FD-Score:3.78|P-value:7.81E-5||SGD DESC:Glycerol 3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase of the glycerolipid biosynthesis pathway, prefers 16-carbon fatty acids, similar to Gpt2p, gene is constitutively transcribed Gene:SLF1(YDR515W)|FD-Score:-3.25|P-value:5.76E-4||SGD DESC:RNA binding protein that associates with polysomes; may be involved in regulating mRNA translation; involved in the copper-dependent mineralization of copper sulfide complexes on cell surface in cells cultured in copper salts; SLF1 has a paralog, SRO9, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SPR28(YDR218C)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Sporulation-specific homolog of the yeast CDC3/10/11/12 family of bud neck microfilament genes; meiotic septin expressed at high levels during meiotic divisions and ascospore formation Gene:SWR1(YDR334W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:TMA23(YMR269W)|FD-Score:3.82|P-value:6.55E-5||SGD DESC:Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan Gene:TPM2(YIL138C)|FD-Score:3.76|P-value:8.52E-5||SGD DESC:Minor isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; appears to have distinct and also overlapping functions with Tpm1p; TPM2 has a paralog, TPM1, that arose from the whole genome duplication Gene:TRM82(YDR165W)|FD-Score:3.25|P-value:5.77E-4||SGD DESC:Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p Gene:TRP4(YDR354W)|FD-Score:3.21|P-value:6.69E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TUL1(YKL034W)|FD-Score:3.13|P-value:8.68E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1 Gene:TUM1(YOR251C)|FD-Score:3.92|P-value:4.52E-5||SGD DESC:Rhodanese domain sulfur transferase, accepts persulfite from Nfs1p and transfers it to Uba4p in the pathway for 2-thiolation of the wobble uridine base of tRNAs; also stimulates sulfur transfer by Nfs1p; may be mitochondrially localized Gene:UBI4(YLL039C)|FD-Score:-4.43|P-value:4.67E-6||SGD DESC:Ubiquitin; becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-26S proteasome system; essential for the cellular stress response; encoded as a polyubiquitin precursor comprised of 5 head-to-tail repeats; protein abundance increases in response to DNA replication stress Gene:UTP30(YKR060W)|FD-Score:-3.12|P-value:8.91E-4||SGD DESC:Putative subunit of U3-containing 90S preribosome complex; complex is involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:VPS4(YPR173C)|FD-Score:3.93|P-value:4.29E-5||SGD DESC:AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism Gene:VPS71(YML041C)|FD-Score:-3.66|P-value:1.25E-4||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YAR023C(YAR023C_p)|FD-Score:-3.52|P-value:2.16E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family Gene:YBR113W(YBR113W_d)|FD-Score:4.64|P-value:1.71E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CYC8 Gene:YBR206W(YBR206W_d)|FD-Score:3.96|P-value:3.74E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KTR3 Gene:YCR007C(YCR007C_p)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene Gene:YDR215C(YDR215C_d)|FD-Score:-3.3|P-value:4.77E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein Gene:YFL012W(YFL012W_p)|FD-Score:3.19|P-value:7.03E-4||SGD DESC:Putative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin Gene:YFL019C(YFL019C_d)|FD-Score:-3.09|P-value:9.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene Gene:YGR125W(YGR125W_p)|FD-Score:3.88|P-value:5.27E-5||SGD DESC:Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YHK8(YHR048W_p)|FD-Score:-3.35|P-value:4.10E-4||SGD DESC:Presumed antiporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; expression of gene is up-regulated in cells exhibiting reduced susceptibility to azoles Gene:YIG1(YPL201C)|FD-Score:3.75|P-value:8.79E-5||SGD DESC:Protein that interacts with glycerol 3-phosphatase and plays a role in anaerobic glycerol production; localizes to the nucleus and cytosol Gene:YJR003C(YJR003C_p)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; predicted to be involved in ribosome biogenesis Gene:YKL118W(YKL118W_d)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YLR342W-A(YLR342W-A_p)|FD-Score:3.75|P-value:8.96E-5||SGD DESC:Putative protein of unknown function Gene:YML007C-A(YML007C-A_p)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YML020W(YML020W_p)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Putative protein of unknown function Gene:YMR001C-A(YMR001C-A_p)|FD-Score:4.2|P-value:1.31E-5||SGD DESC:Putative protein of unknown function Gene:YNL095C(YNL095C_p)|FD-Score:-4.66|P-value:1.59E-6||SGD DESC:Putative protein of unknown function; predicted to contain a transmembrane domain; not an essential gene; YNL095C has a paralog, ECM3, that arose from the whole genome duplication Gene:YNL338W(YNL338W_d)|FD-Score:3.18|P-value:7.44E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps TEL14L-XC, which is Telomeric X element Core sequence on the left arm of Chromosome XIV Gene:YNR064C(YNR064C)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Epoxide hydrolase, member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides Gene:YOR055W(YOR055W_d)|FD-Score:3.26|P-value:5.55E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPK9(YOR291W)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-type ATPase Spf1p; homolog of human ATP13A2 (PARK9), mutations in which are associated with Parkinson disease and Kufor-Rakeb syndrome Gene:YSR3(YKR053C)|FD-Score:4.01|P-value:3.09E-5||SGD DESC:Dihydrosphingosine 1-phosphate phosphatase; membrane protein involved in sphingolipid metabolism; YSR3 has a paralog, LCB3, that arose from the whole genome duplication

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YNL172W4.121.87E-50.72APC1Largest subunit of the Anaphase-Promoting Complex/Cyclosome; APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; localizes to nuclear foci that become diffuse upon DNA replication stress
YGR114C_d4.042.66E-50.72YGR114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
YLR459W3.836.38E-50.72GAB1GPI transamidase subunit, involved in attachment of glycosylphosphatidylinositol (GPI) anchors to proteins; may have a role in recognition of the attachment signal or of the lipid portion of GPI
YKL060C3.119.47E-40.02FBA1Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversion of fructose 1,6 bisphosphate to glyceraldehyde-3-P and dihydroxyacetone-P; locates to mitochondrial outer surface upon oxidative stress
YOR176W3.090.001000.17HEM15Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway
YML114C2.920.001770.12TAF8TFIID subunit (65 kDa), involved in RNA polymerase II transcription initiation
YJL104W2.800.002570.13PAM16Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); forms a 1:1 subcomplex with Pam18p and inhibits its cochaperone activity; contains a J-like domain
YER009W2.670.003760.04NTF2Nuclear envelope protein, interacts with GDP-bound Gsp1p and with proteins of the nuclear pore to transport Gsp1p into the nucleus where it is an essential player in nucleocytoplasmic transport
YDR365C2.630.004250.07ESF1Nucleolar protein involved in pre-rRNA processing; depletion causes severely decreased 18S rRNA levels
YKL028W2.560.005290.06TFA1TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening
YGL123W2.490.006340.02RPS2Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5
YIL003W2.480.006650.02CFD1Highly conserved, iron-sulfur cluster binding protein localized in the cytoplasm; forms a complex with Nbp35p that is involved in iron-sulfur protein assembly in the cytosol
YIL142W2.460.006940.02CCT2Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YHR069C2.440.007260.01RRP4Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2)
YKL036C_d2.440.007370.03YKL036C_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YNL041C8.421.83E-17COG6Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YBL089W6.173.35E-10AVT5Putative transporter; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters; AVT5 has a paralog, AVT6, that arose from the whole genome duplication
YGL005C5.832.76E-9COG7Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YIL117C5.783.68E-9PRM5Pheromone-regulated protein, predicted to have 1 transmembrane segment; induced during cell integrity signaling
YDR384C5.482.10E-8ATO3Plasma membrane protein, regulation pattern suggests a possible role in export of ammonia from the cell; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family of putative transporters
YHL024W5.373.91E-8RIM4Putative RNA-binding protein required for the expression of early and middle sporulation genes
YNL289W5.373.92E-8PCL1Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; is ubiquitinated by Dma1p; phosphorylation by Pho85p targets it for degradation; localizes to sites of polarized cell growth
YKR097W5.209.87E-8PCK1Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol
YBR072W4.865.79E-7HSP26Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; oligomer activation requires heat-induced conformational change; also has mRNA binding activity
YNL082W4.769.65E-7PMS1ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL
YBR286W4.701.32E-6APE3Vacuolar aminopeptidase Y, processed to mature form by Prb1p
YIL124W4.661.56E-6AYR1NADPH-dependent 1-acyl dihydroxyacetone phosphate reductase found in lipid particles, ER, and mitochondrial outer membrane; involved in phosphatidic acid biosynthesis; required for spore germination; capable of metabolizing steroid hormones
YBR113W_d4.641.71E-6YBR113W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CYC8
YLR191W4.592.26E-6PEX13Integral peroxisomal membrane protein required for translocation of peroxisomal matrix proteins, interacts with the PTS1 signal recognition factor Pex5p and the PTS2 signal recognition factor Pex7p, forms a complex with Pex14p and Pex17p
YOL071W4.434.70E-6EMI5Subunit of succinate dehydrogenase, which couples succinate oxidation to ubiquinone reduction; required for FAD cofactor attachment to Sdh1p; mutations in human ortholog PGL2 are associated with neuroendocrine tumors (paraganglioma)

GO enrichment analysis for SGTC_271
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1157.97E-19SGTC_1162k048-0130 20.1 μMChemDiv (Drug-like library)67974060.047619Golgi
0.0953.22E-13SGTC_6320781-2353 4.7 μMChemDiv (Drug-like library)68115150.0408163Golgi
0.0937.58E-13SGTC_10603448-7350 82.0 μMChemDiv (Drug-like library)5701780.0769231Golgi
0.0897.36E-12SGTC_2737bleomycin 30.5 nMMiscellaneous162113180.042328Golgi
0.0872.44E-11SGTC_250brefeldin a 552.2 μMICCB bioactive library64361870.104651PDR1
0.0841.02E-10SGTC_1985st071382 65.5 μMTimTec (Natural product derivative library)61232370.134146
0.0831.55E-10SGTC_342k048-0088 23.3 μMChemDiv (Drug-like library)68205700.0795455RSC & ERG11
0.0822.20E-10SGTC_7371950-0117 57.9 μMChemDiv (Drug-like library)53935130.0537634
0.0815.31E-10SGTC_40960-0234 25.7 μMChemDiv (Drug-like library)68211120.0721649Golgi
0.0791.02E-9SGTC_624k048-0052 28.0 μMChemDiv (Drug-like library)68355310.0666667Golgi
0.0772.93E-9SGTC_7461095-0025 46.6 μMChemDiv (Drug-like library)67492860.0465116RSC & ERG11
0.0741.21E-8SGTC_1139k015-0024 46.9 μMChemDiv (Drug-like library)27666520.0833333PDR1
0.0732.14E-8SGTC_9332914-0560 114.0 μMChemDiv (Drug-like library)4638870.037037
0.0722.64E-8SGTC_30579095946 49.5 μMChembridge (Drug-like library)245400430.103448Golgi
0.0723.55E-8SGTC_20405100729 123.0 μMChembridge (Fragment library)118400.114286

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2619obliquin87.91 μM0.3142866708593Microsource (Natural product library)244.242682.77704cell wall
SGTC_15382',3',6-trimethoxyflavanone63.6 μM0.2763164213774TimTec (Pure natural product library)314.332523.0505
SGTC_2603homopterocarpin100 μM0.26087101795Microsource (Natural product library)284.306542.90804
SGTC_2559tetrahydrosappanone a trimethyl ether100 μM0.259746708784Microsource (Natural product library)330.374983.00615
SGTC_5313788-1495174 μM0.2467532896655ChemDiv (Drug-like library)299.321182.37814unfolded protein response
SGTC_3135912774249.47 μM0.2439023643468Chembridge (Drug-like library)427.45041.77316
SGTC_1518st03856790.8 μM0.24242477966TimTec (Pure natural product library)220.221282.31304
SGTC_1866st05763864 μM0.236111688802TimTec (Natural product derivative library)312.316643.08705
SGTC_2714st07931356.9 μM0.2337662939799TimTec (Natural product derivative library)351.319753.46916
SGTC_15465-methoxyflavanone78.7 μM0.232877147795TimTec (Pure natural product library)254.280563.08303