st078562

2-[(9-ethyl-6-oxo-3H-purin-8-yl)sulfanyl]-N-(2-ethylphenyl)acetamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2710
Screen concentration 53.7 μM
Source TimTec (Natural product derivative library)
PubChem CID 17250908
SMILES CCC1=CC=CC=C1NC(=O)CSC2=NC3=C(N2CC)NC=NC3=O
Standardized SMILES CCc1ccccc1NC(=O)CSc2nc3c(O)ncnc3n2CC
Molecular weight 357.4301
ALogP 2.41
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.51
% growth inhibition (Hom. pool) 1.18


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 17250908
Download HIP data (tab-delimited text)  (excel)
Gene:ABD1(YBR236C)|FD-Score:4.16|P-value:1.59E-5|Clearance:0.23||SGD DESC:Methyltransferase, catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA Gene:CCT2(YIL142W)|FD-Score:3.13|P-value:8.85E-4|Clearance:0||SGD DESC:Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT4(YDL143W)|FD-Score:5.06|P-value:2.09E-7|Clearance:0.56||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC48(YDL126C)|FD-Score:3.91|P-value:4.56E-5|Clearance:0.01||SGD DESC:AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP Gene:DBP2(YNL112W)|FD-Score:-6.89|P-value:2.77E-12|Clearance:0||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:DPB11(YJL090C)|FD-Score:3.19|P-value:7.08E-4|Clearance:0.06||SGD DESC:DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress Gene:DRS1(YLL008W)|FD-Score:3.85|P-value:6.02E-5|Clearance:0.17||SGD DESC:Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles Gene:HSH49(YOR319W)|FD-Score:3.93|P-value:4.31E-5|Clearance:0.01||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:MMS21(YEL019C)|FD-Score:3.13|P-value:8.81E-4|Clearance:0||SGD DESC:SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination Gene:NOG2(YNR053C)|FD-Score:4.5|P-value:3.39E-6|Clearance:0.18||SGD DESC:Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation Gene:PRP24(YMR268C)|FD-Score:3.36|P-value:3.84E-4|Clearance:0.07||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:RER2(YBR002C)|FD-Score:3.91|P-value:4.66E-5|Clearance:0.06||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RPA43(YOR340C)|FD-Score:-3.23|P-value:6.23E-4|Clearance:0||SGD DESC:RNA polymerase I subunit A43 Gene:RPN5(YDL147W)|FD-Score:3.29|P-value:5.00E-4|Clearance:0.1||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:SEC13(YLR208W)|FD-Score:-4.01|P-value:2.99E-5|Clearance:0||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC3(YER008C)|FD-Score:3.12|P-value:9.18E-4|Clearance:0.16||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress Gene:SPT15(YER148W)|FD-Score:3.68|P-value:1.17E-4|Clearance:0.03||SGD DESC:TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability Gene:TOM40(YMR203W)|FD-Score:-4.43|P-value:4.65E-6|Clearance:0||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:UGP1(YKL035W)|FD-Score:-3.93|P-value:4.20E-5|Clearance:0||SGD DESC:UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication Gene:UTP6(YDR449C)|FD-Score:3.65|P-value:1.29E-4|Clearance:0.29||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YDR187C(YDR187C_d)|FD-Score:4.32|P-value:7.72E-6|Clearance:0.16||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YGR114C(YGR114C_d)|FD-Score:3.12|P-value:8.93E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:ABD1(YBR236C)|FD-Score:4.16|P-value:1.59E-5|Clearance:0.23||SGD DESC:Methyltransferase, catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA Gene:CCT2(YIL142W)|FD-Score:3.13|P-value:8.85E-4|Clearance:0||SGD DESC:Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT4(YDL143W)|FD-Score:5.06|P-value:2.09E-7|Clearance:0.56||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC48(YDL126C)|FD-Score:3.91|P-value:4.56E-5|Clearance:0.01||SGD DESC:AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP Gene:DBP2(YNL112W)|FD-Score:-6.89|P-value:2.77E-12|Clearance:0||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:DPB11(YJL090C)|FD-Score:3.19|P-value:7.08E-4|Clearance:0.06||SGD DESC:DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress Gene:DRS1(YLL008W)|FD-Score:3.85|P-value:6.02E-5|Clearance:0.17||SGD DESC:Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles Gene:HSH49(YOR319W)|FD-Score:3.93|P-value:4.31E-5|Clearance:0.01||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:MMS21(YEL019C)|FD-Score:3.13|P-value:8.81E-4|Clearance:0||SGD DESC:SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination Gene:NOG2(YNR053C)|FD-Score:4.5|P-value:3.39E-6|Clearance:0.18||SGD DESC:Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation Gene:PRP24(YMR268C)|FD-Score:3.36|P-value:3.84E-4|Clearance:0.07||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:RER2(YBR002C)|FD-Score:3.91|P-value:4.66E-5|Clearance:0.06||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RPA43(YOR340C)|FD-Score:-3.23|P-value:6.23E-4|Clearance:0||SGD DESC:RNA polymerase I subunit A43 Gene:RPN5(YDL147W)|FD-Score:3.29|P-value:5.00E-4|Clearance:0.1||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:SEC13(YLR208W)|FD-Score:-4.01|P-value:2.99E-5|Clearance:0||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC3(YER008C)|FD-Score:3.12|P-value:9.18E-4|Clearance:0.16||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress Gene:SPT15(YER148W)|FD-Score:3.68|P-value:1.17E-4|Clearance:0.03||SGD DESC:TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability Gene:TOM40(YMR203W)|FD-Score:-4.43|P-value:4.65E-6|Clearance:0||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:UGP1(YKL035W)|FD-Score:-3.93|P-value:4.20E-5|Clearance:0||SGD DESC:UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication Gene:UTP6(YDR449C)|FD-Score:3.65|P-value:1.29E-4|Clearance:0.29||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YDR187C(YDR187C_d)|FD-Score:4.32|P-value:7.72E-6|Clearance:0.16||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YGR114C(YGR114C_d)|FD-Score:3.12|P-value:8.93E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 17250908
Download HOP data (tab-delimited text)  (excel)
Gene:ACS1(YAL054C)|FD-Score:-5.75|P-value:4.59E-9||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs2p, is the nuclear source of acetyl-coA for histone acetylation; expressed during growth on nonfermentable carbon sources and under aerobic conditions Gene:AGP1(YCL025C)|FD-Score:3.86|P-value:5.74E-5||SGD DESC:Low-affinity amino acid permease with broad substrate range; involved in uptake of asparagine, glutamine, and other amino acids; expression regulated by SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); AGP1 has a paralog, GNP1, that arose from the whole genome duplication Gene:ASI2(YNL159C)|FD-Score:3.2|P-value:6.75E-4||SGD DESC:Integral inner nuclear membrane protein that acts with Asi1p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals Gene:BRE5(YNR051C)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A Gene:BUD26(YDR241W_d)|FD-Score:3.83|P-value:6.49E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 1% of ORF overlaps the verified gene SNU56; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CKA2(YOR061W)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching Gene:COQ1(YBR003W)|FD-Score:3.81|P-value:7.08E-5||SGD DESC:Hexaprenyl pyrophosphate synthetase, catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis Gene:CWC21(YDR482C)|FD-Score:4.46|P-value:4.16E-6||SGD DESC:Protein involved in RNA splicing by the spliceosome; component of a complex containing Cef1p; interacts genetically with ISY1 and BUD13; may bind RNA; has similarity to S. pombe Cwf21p Gene:DOC1(YGL240W)|FD-Score:3.14|P-value:8.51E-4||SGD DESC:Processivity factor required for the ubiquitination activity of the anaphase promoting complex (APC), mediates the activity of the APC by contributing to substrate recognition; involved in cyclin proteolysis; contains a conserved DOC1 homology domain Gene:ENV9(YOR246C)|FD-Score:6.61|P-value:1.93E-11||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:GLO2(YDR272W)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Cytoplasmic glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate Gene:GLY1(YEL046C)|FD-Score:4.07|P-value:2.38E-5||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GND2(YGR256W)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone; GND2 has a paralog, GND1, that arose from the whole genome duplication Gene:GPM2(YDL021W)|FD-Score:-3.25|P-value:5.67E-4||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:GRR1(YJR090C)|FD-Score:3.81|P-value:6.96E-5||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:GSP2(YOR185C)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); Gsp1p homolog that is not required for viability; protein abundance increases in response to DNA replication stress Gene:HAL5(YJL165C)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication Gene:HXT1(YHR094C)|FD-Score:-3.56|P-value:1.88E-4||SGD DESC:Low-affinity glucose transporter of the major facilitator superfamily, expression is induced by Hxk2p in the presence of glucose and repressed by Rgt1p when glucose is limiting; HXT1 has a paralog, HXT6, what arose from the whole genome duplication Gene:KAR5(YMR065W)|FD-Score:4.35|P-value:6.81E-6||SGD DESC:Protein required for nuclear membrane fusion during karyogamy, localizes to the membrane with a soluble portion in the endoplasmic reticulum lumen, may form a complex with Jem1p and Kar2p; expression of the gene is regulated by pheromone Gene:MAD3(YJL013C)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Subunit of the spindle-assembly checkpoint complex, which delays anaphase onset in cells with defects in mitotic spindle assembly; pseudosubstrate inhibitor of APC(Cdc20), the anaphase promoting complex involved in securin (Pds1p) turnover Gene:MAG2(YLR427W)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Cytoplasmic protein of unknown function; induced in response to mycotoxin patulin; ubiquitinated protein similar to the human ring finger motif protein RNF10; predicted to be involved in repair of alkylated DNA due to interaction with MAG1 Gene:MIP6(YHR015W)|FD-Score:3.93|P-value:4.18E-5||SGD DESC:Putative RNA-binding protein; interacts with Mex67p, which is a component of the nuclear pore involved in nuclear mRNA export; MIP6 has a paralog, PES4, that arose from the whole genome duplication Gene:MNL1(YHR204W)|FD-Score:4.1|P-value:2.11E-5||SGD DESC:Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; in complex with Pdi1p, generates a Man7GlcNac2 oligosaccharide signal on glycoproteins destined for ubiquitin-proteasome degradation Gene:MRPL33(YMR286W)|FD-Score:-3.58|P-value:1.71E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSC1(YML128C)|FD-Score:-4.3|P-value:8.52E-6||SGD DESC:Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria and is phosphorylated Gene:NUM1(YDR150W)|FD-Score:-5.31|P-value:5.57E-8||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:NUP60(YAR002W)|FD-Score:4.29|P-value:9.10E-6||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; both NUP1 and NUP60 are homologous to human NUP153 Gene:PAC10(YGR078C)|FD-Score:4.6|P-value:2.10E-6||SGD DESC:Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding Gene:PHO80(YOL001W)|FD-Score:4.52|P-value:3.04E-6||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling Gene:PRM8(YGL053W)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Pheromone-regulated protein with 2 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; forms a complex with Prp9p in the ER; member of DUP240 gene family Gene:RAV1(YJR033C)|FD-Score:5.06|P-value:2.10E-7||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:SEG1(YMR086W)|FD-Score:-3.26|P-value:5.57E-4||SGD DESC:Component of eisosome required for proper eisosome assembly; precedes Pil1p/Lsp1p during eisosome formation and controls eisosome length and shape; Seg1p-GFP diffusely distributed and forms heterogeneous patches at the plasma membrane in small buds, also found in medium and large buds; may interact with ribosomes, based on co-purification experiments; expression is repressed by cAMP; similar to A. gossypii SEG gene and to S. pombe Sle1p, important for generating eisosomes Gene:SKN1(YGR143W)|FD-Score:4.05|P-value:2.58E-5||SGD DESC:Protein involved in sphingolipid biosynthesis; type II membrane protein with similarity to Kre6p Gene:SRL1(YOR247W)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication Gene:SRO77(YBL106C)|FD-Score:5.09|P-value:1.78E-7||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:STP1(YDR463W)|FD-Score:4.3|P-value:8.63E-6||SGD DESC:Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication Gene:STP22(YCL008C)|FD-Score:-3.16|P-value:8.02E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:THP1(YOL072W)|FD-Score:3.26|P-value:5.50E-4||SGD DESC:Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding Gene:TIR1(YER011W)|FD-Score:-3.1|P-value:9.67E-4||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expression is downregulated at acidic pH and induced by cold shock and anaerobiosis; abundance is increased in cells cultured without shaking Gene:TRP2(YER090W)|FD-Score:3.79|P-value:7.45E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:UGX2(YDL169C)|FD-Score:3.81|P-value:6.85E-5||SGD DESC:Protein of unknown function, transcript accumulates in response to any combination of stress conditions Gene:UTR1(YJR049C)|FD-Score:-3.72|P-value:9.82E-5||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:WSC2(YNL283C)|FD-Score:5.69|P-value:6.49E-9||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response Gene:XRS2(YDR369C)|FD-Score:3.46|P-value:2.73E-4||SGD DESC:Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling Gene:YDL041W(YDL041W_d)|FD-Score:4.4|P-value:5.40E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YDR090C(YDR090C_p)|FD-Score:3.77|P-value:8.32E-5||SGD DESC:Putative protein of unknown function Gene:YET3(YDL072C)|FD-Score:-3.28|P-value:5.19E-4||SGD DESC:Protein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein; protein abundance increases in response to DNA replication stress Gene:YHR127W(YHR127W)|FD-Score:-4.29|P-value:9.10E-6||SGD DESC:Protein of unknown function; localizes to the nucleus; required for asymmetric localization of Kar9p during mitosis Gene:YLR422W(YLR422W_p)|FD-Score:4.13|P-value:1.82E-5||SGD DESC:Protein of unknown function with similarity to human DOCK proteins (guanine nucleotide exchange factors); interacts with Ino4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, YLR422W is not an essential protein Gene:YML007C-A(YML007C-A_p)|FD-Score:3.11|P-value:9.48E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YMR119W-A(YMR119W-A_d)|FD-Score:3.84|P-value:6.12E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL034W(YNL034W_p)|FD-Score:-3.25|P-value:5.72E-4||SGD DESC:Putative protein of unknown function; YNL034W is not an essential gene Gene:YPR084W(YPR084W_p)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Putative protein of unknown function Gene:YRR1(YOR162C)|FD-Score:-3.28|P-value:5.20E-4||SGD DESC:Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication Gene:ACS1(YAL054C)|FD-Score:-5.75|P-value:4.59E-9||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs2p, is the nuclear source of acetyl-coA for histone acetylation; expressed during growth on nonfermentable carbon sources and under aerobic conditions Gene:AGP1(YCL025C)|FD-Score:3.86|P-value:5.74E-5||SGD DESC:Low-affinity amino acid permease with broad substrate range; involved in uptake of asparagine, glutamine, and other amino acids; expression regulated by SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); AGP1 has a paralog, GNP1, that arose from the whole genome duplication Gene:ASI2(YNL159C)|FD-Score:3.2|P-value:6.75E-4||SGD DESC:Integral inner nuclear membrane protein that acts with Asi1p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals Gene:BRE5(YNR051C)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A Gene:BUD26(YDR241W_d)|FD-Score:3.83|P-value:6.49E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 1% of ORF overlaps the verified gene SNU56; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CKA2(YOR061W)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching Gene:COQ1(YBR003W)|FD-Score:3.81|P-value:7.08E-5||SGD DESC:Hexaprenyl pyrophosphate synthetase, catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis Gene:CWC21(YDR482C)|FD-Score:4.46|P-value:4.16E-6||SGD DESC:Protein involved in RNA splicing by the spliceosome; component of a complex containing Cef1p; interacts genetically with ISY1 and BUD13; may bind RNA; has similarity to S. pombe Cwf21p Gene:DOC1(YGL240W)|FD-Score:3.14|P-value:8.51E-4||SGD DESC:Processivity factor required for the ubiquitination activity of the anaphase promoting complex (APC), mediates the activity of the APC by contributing to substrate recognition; involved in cyclin proteolysis; contains a conserved DOC1 homology domain Gene:ENV9(YOR246C)|FD-Score:6.61|P-value:1.93E-11||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:GLO2(YDR272W)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Cytoplasmic glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate Gene:GLY1(YEL046C)|FD-Score:4.07|P-value:2.38E-5||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GND2(YGR256W)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone; GND2 has a paralog, GND1, that arose from the whole genome duplication Gene:GPM2(YDL021W)|FD-Score:-3.25|P-value:5.67E-4||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:GRR1(YJR090C)|FD-Score:3.81|P-value:6.96E-5||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:GSP2(YOR185C)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); Gsp1p homolog that is not required for viability; protein abundance increases in response to DNA replication stress Gene:HAL5(YJL165C)|FD-Score:3.17|P-value:7.53E-4||SGD DESC:Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication Gene:HXT1(YHR094C)|FD-Score:-3.56|P-value:1.88E-4||SGD DESC:Low-affinity glucose transporter of the major facilitator superfamily, expression is induced by Hxk2p in the presence of glucose and repressed by Rgt1p when glucose is limiting; HXT1 has a paralog, HXT6, what arose from the whole genome duplication Gene:KAR5(YMR065W)|FD-Score:4.35|P-value:6.81E-6||SGD DESC:Protein required for nuclear membrane fusion during karyogamy, localizes to the membrane with a soluble portion in the endoplasmic reticulum lumen, may form a complex with Jem1p and Kar2p; expression of the gene is regulated by pheromone Gene:MAD3(YJL013C)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Subunit of the spindle-assembly checkpoint complex, which delays anaphase onset in cells with defects in mitotic spindle assembly; pseudosubstrate inhibitor of APC(Cdc20), the anaphase promoting complex involved in securin (Pds1p) turnover Gene:MAG2(YLR427W)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Cytoplasmic protein of unknown function; induced in response to mycotoxin patulin; ubiquitinated protein similar to the human ring finger motif protein RNF10; predicted to be involved in repair of alkylated DNA due to interaction with MAG1 Gene:MIP6(YHR015W)|FD-Score:3.93|P-value:4.18E-5||SGD DESC:Putative RNA-binding protein; interacts with Mex67p, which is a component of the nuclear pore involved in nuclear mRNA export; MIP6 has a paralog, PES4, that arose from the whole genome duplication Gene:MNL1(YHR204W)|FD-Score:4.1|P-value:2.11E-5||SGD DESC:Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; in complex with Pdi1p, generates a Man7GlcNac2 oligosaccharide signal on glycoproteins destined for ubiquitin-proteasome degradation Gene:MRPL33(YMR286W)|FD-Score:-3.58|P-value:1.71E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSC1(YML128C)|FD-Score:-4.3|P-value:8.52E-6||SGD DESC:Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria and is phosphorylated Gene:NUM1(YDR150W)|FD-Score:-5.31|P-value:5.57E-8||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:NUP60(YAR002W)|FD-Score:4.29|P-value:9.10E-6||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; both NUP1 and NUP60 are homologous to human NUP153 Gene:PAC10(YGR078C)|FD-Score:4.6|P-value:2.10E-6||SGD DESC:Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding Gene:PHO80(YOL001W)|FD-Score:4.52|P-value:3.04E-6||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling Gene:PRM8(YGL053W)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Pheromone-regulated protein with 2 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; forms a complex with Prp9p in the ER; member of DUP240 gene family Gene:RAV1(YJR033C)|FD-Score:5.06|P-value:2.10E-7||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:SEG1(YMR086W)|FD-Score:-3.26|P-value:5.57E-4||SGD DESC:Component of eisosome required for proper eisosome assembly; precedes Pil1p/Lsp1p during eisosome formation and controls eisosome length and shape; Seg1p-GFP diffusely distributed and forms heterogeneous patches at the plasma membrane in small buds, also found in medium and large buds; may interact with ribosomes, based on co-purification experiments; expression is repressed by cAMP; similar to A. gossypii SEG gene and to S. pombe Sle1p, important for generating eisosomes Gene:SKN1(YGR143W)|FD-Score:4.05|P-value:2.58E-5||SGD DESC:Protein involved in sphingolipid biosynthesis; type II membrane protein with similarity to Kre6p Gene:SRL1(YOR247W)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication Gene:SRO77(YBL106C)|FD-Score:5.09|P-value:1.78E-7||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:STP1(YDR463W)|FD-Score:4.3|P-value:8.63E-6||SGD DESC:Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication Gene:STP22(YCL008C)|FD-Score:-3.16|P-value:8.02E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:THP1(YOL072W)|FD-Score:3.26|P-value:5.50E-4||SGD DESC:Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding Gene:TIR1(YER011W)|FD-Score:-3.1|P-value:9.67E-4||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expression is downregulated at acidic pH and induced by cold shock and anaerobiosis; abundance is increased in cells cultured without shaking Gene:TRP2(YER090W)|FD-Score:3.79|P-value:7.45E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:UGX2(YDL169C)|FD-Score:3.81|P-value:6.85E-5||SGD DESC:Protein of unknown function, transcript accumulates in response to any combination of stress conditions Gene:UTR1(YJR049C)|FD-Score:-3.72|P-value:9.82E-5||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:WSC2(YNL283C)|FD-Score:5.69|P-value:6.49E-9||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response Gene:XRS2(YDR369C)|FD-Score:3.46|P-value:2.73E-4||SGD DESC:Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling Gene:YDL041W(YDL041W_d)|FD-Score:4.4|P-value:5.40E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YDR090C(YDR090C_p)|FD-Score:3.77|P-value:8.32E-5||SGD DESC:Putative protein of unknown function Gene:YET3(YDL072C)|FD-Score:-3.28|P-value:5.19E-4||SGD DESC:Protein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein; protein abundance increases in response to DNA replication stress Gene:YHR127W(YHR127W)|FD-Score:-4.29|P-value:9.10E-6||SGD DESC:Protein of unknown function; localizes to the nucleus; required for asymmetric localization of Kar9p during mitosis Gene:YLR422W(YLR422W_p)|FD-Score:4.13|P-value:1.82E-5||SGD DESC:Protein of unknown function with similarity to human DOCK proteins (guanine nucleotide exchange factors); interacts with Ino4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, YLR422W is not an essential protein Gene:YML007C-A(YML007C-A_p)|FD-Score:3.11|P-value:9.48E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YMR119W-A(YMR119W-A_d)|FD-Score:3.84|P-value:6.12E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL034W(YNL034W_p)|FD-Score:-3.25|P-value:5.72E-4||SGD DESC:Putative protein of unknown function; YNL034W is not an essential gene Gene:YPR084W(YPR084W_p)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Putative protein of unknown function Gene:YRR1(YOR162C)|FD-Score:-3.28|P-value:5.20E-4||SGD DESC:Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL143W5.062.09E-70.56CCT4Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YNR053C4.503.39E-60.18NOG2Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation
YDR187C_d4.327.72E-60.16YDR187C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W
YBR236C4.161.59E-50.23ABD1Methyltransferase, catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA
YOR319W3.934.31E-50.01HSH49U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM)
YDL126C3.914.56E-50.01CDC48AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP
YBR002C3.914.66E-50.06RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YLL008W3.856.02E-50.17DRS1Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles
YER148W3.681.17E-40.03SPT15TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability
YDR449C3.651.29E-40.29UTP6Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YMR268C3.363.84E-40.07PRP24Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling
YDL147W3.295.00E-40.10RPN5Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein
YJL090C3.197.08E-40.06DPB11DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress
YEL019C3.138.81E-40.00MMS21SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination
YIL142W3.138.85E-40.00CCT2Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOR246C6.611.93E-11ENV9Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts
YNL283C5.696.49E-9WSC2Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response
YBL106C5.091.78E-7SRO77Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication
YJR033C5.062.10E-7RAV1Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate
YGR078C4.602.10E-6PAC10Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding
YOL001W4.523.04E-6PHO80Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling
YDR482C4.464.16E-6CWC21Protein involved in RNA splicing by the spliceosome; component of a complex containing Cef1p; interacts genetically with ISY1 and BUD13; may bind RNA; has similarity to S. pombe Cwf21p
YDL041W_d4.405.40E-6YDL041W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C
YMR065W4.356.81E-6KAR5Protein required for nuclear membrane fusion during karyogamy, localizes to the membrane with a soluble portion in the endoplasmic reticulum lumen, may form a complex with Jem1p and Kar2p; expression of the gene is regulated by pheromone
YDR463W4.308.63E-6STP1Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication
YAR002W4.299.10E-6NUP60FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; both NUP1 and NUP60 are homologous to human NUP153
YLR422W_p4.131.82E-5YLR422W_pProtein of unknown function with similarity to human DOCK proteins (guanine nucleotide exchange factors); interacts with Ino4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, YLR422W is not an essential protein
YHR204W4.102.11E-5MNL1Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; in complex with Pdi1p, generates a Man7GlcNac2 oligosaccharide signal on glycoproteins destined for ubiquitin-proteasome degradation
YEL046C4.072.38E-5GLY1Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis
YGR143W4.052.58E-5SKN1Protein involved in sphingolipid biosynthesis; type II membrane protein with similarity to Kre6p

GO enrichment analysis for SGTC_2710
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1198.27E-20SGTC_2094077-0173 175.6 μMChemDiv (Drug-like library)159934940.0689655
0.0895.98E-12SGTC_13021159-0335 255.0 μMChemDiv (Drug-like library)6492840.1375
0.0872.00E-11SGTC_2715st079500 56.4 μMTimTec (Natural product derivative library)282466880.0978261
0.0832.05E-10SGTC_21545570129 200.0 μMChembridge (Fragment library)6733660.328358
0.0832.13E-10SGTC_2413inermin 26.1 μMTimTec (Natural product derivative library)915100.0348837
0.0806.75E-10SGTC_14294049-0205 9.5 μMChemDiv (Drug-like library)227744780.075
0.0791.48E-9SGTC_1871488-2104 32.7 μMChemDiv (Drug-like library)28528590.1calcium & mitochondrial duress
0.0764.49E-9SGTC_28839059145 45.5 μMChembridge (Drug-like library)164765100.120482
0.0765.69E-9SGTC_23047443016 91.4 μMChembridge (Fragment library)9398540.0853659
0.0714.24E-8SGTC_1774st048371 48.5 μMTimTec (Natural product derivative library)42427270.139241RNA processing & uracil transport
0.0691.16E-7SGTC_21635671233 125.0 μMChembridge (Fragment library)7010230.0833333
0.0691.16E-7SGTC_2712st079126 47.7 μMTimTec (Natural product derivative library)31529700.130435
0.0681.78E-7SGTC_3364111-0029 111.0 μMChemDiv (Drug-like library)31311750.0659341
0.0673.10E-7SGTC_2705st078069 42.8 μMTimTec (Natural product derivative library)15300610.11236
0.0664.41E-7SGTC_29679089538 49.9 μMChembridge (Drug-like library)7282160.120482

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_2708st07855956 μM0.75409817250902TimTec (Natural product derivative library)357.430062.43724
SGTC_2709st07856056 μM0.69841317250903TimTec (Natural product derivative library)357.430062.43724
SGTC_1944st07637156.9 μM0.53623224208455TimTec (Natural product derivative library)351.3312461.52726
SGTC_2707st07855666.6 μM0.5238117250899TimTec (Natural product derivative library)300.378742.89813
SGTC_2692st07742647.7 μM0.51388925238571TimTec (Natural product derivative library)419.499444.12124
SGTC_1943st07633165.2 μM0.44776124208415TimTec (Natural product derivative library)306.770642.72813
SGTC_1948st07632966.6 μM0.38028224208413TimTec (Natural product derivative library)300.378743.03613
SGTC_1942st07633065.2 μM0.37681224208414TimTec (Natural product derivative library)306.770642.72813
SGTC_1947st07633261.6 μM0.36486524208416TimTec (Natural product derivative library)324.7611032.93314
SGTC_552k242-0005476 μM0.3378384088318ChemDiv (Drug-like library)419.926525.8313