st079126

methyl 4-[4-(2-methoxy-2-oxoethoxy)phenyl]-2-methyl-5-oxo-1,4-dihydroindeno[1,2-b]pyridine-3-carboxylate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2712
Screen concentration 47.7 μM
Source TimTec (Natural product derivative library)
PubChem CID 3152970
SMILES CC1=C(C(C2=C(N1)C3=CC=CC=C3C2=O)C4=CC=C(C=C4)OCC(=O)OC)C(=O)OC
Standardized SMILES COC(=O)COc1ccc(cc1)C2C(=C(C)N=C3C2=C(O)c4ccccc34)C(=O)OC
Molecular weight 419.4266
ALogP 2.52
H-bond donor count 1
H-bond acceptor count 7
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 4.01
% growth inhibition (Hom. pool) -1.26


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3152970
Download HIP data (tab-delimited text)  (excel)
Gene:ACT1(YFL039C)|FD-Score:3.45|P-value:2.76E-4|Clearance:0.03||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:BCP1(YDR361C)|FD-Score:3.13|P-value:8.87E-4|Clearance:0||SGD DESC:Essential protein involved in nuclear export of Mss4p, which is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport Gene:BET4(YJL031C)|FD-Score:3.61|P-value:1.52E-4|Clearance:0.01||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:ENP2(YGR145W)|FD-Score:3.59|P-value:1.65E-4|Clearance:0.14||SGD DESC:Essential nucleolar protein, required for biogenesis of the small ribosomal subunit; contains WD repeats, interacts with Mpp10p and Bfr2p, and has homology to Spb1p Gene:GPI11(YDR302W)|FD-Score:3.81|P-value:7.06E-5|Clearance:0.19||SGD DESC:ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog Gene:MMS21(YEL019C)|FD-Score:3.86|P-value:5.58E-5|Clearance:0.06||SGD DESC:SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination Gene:MYO2(YOR326W)|FD-Score:-4.73|P-value:1.11E-6|Clearance:0||SGD DESC:Type V myosin motor involved in actin-based transport of cargos; required for the polarized delivery of secretory vesicles, the vacuole, late Golgi elements, peroxisomes, and the mitotic spindle; MYO2 has a paralog, MYO4, that arose from the whole genome duplication Gene:NOC2(YOR206W)|FD-Score:4.27|P-value:9.92E-6|Clearance:0.4||SGD DESC:Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors Gene:ORC3(YLL004W)|FD-Score:-3.16|P-value:8.00E-4|Clearance:0||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:PDS1(YDR113C)|FD-Score:3.2|P-value:6.92E-4|Clearance:0.07||SGD DESC:Securin, inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:PRE8(YML092C)|FD-Score:-4.37|P-value:6.27E-6|Clearance:0||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:RRN6(YBL014C)|FD-Score:3.13|P-value:8.65E-4|Clearance:0.01||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:RVB2(YPL235W)|FD-Score:4.69|P-value:1.35E-6|Clearance:0.43||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SKI6(YGR195W)|FD-Score:3.42|P-value:3.12E-4|Clearance:0.22||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4) Gene:TRE2(YOR256C)|FD-Score:3.13|P-value:8.89E-4|Clearance:0.05||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication Gene:UGP1(YKL035W)|FD-Score:-4.64|P-value:1.71E-6|Clearance:0||SGD DESC:UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication Gene:YAH1(YPL252C)|FD-Score:-4.61|P-value:2.03E-6|Clearance:0||SGD DESC:Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin Gene:YLR076C(YLR076C_d)|FD-Score:3.6|P-value:1.59E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 Gene:ACT1(YFL039C)|FD-Score:3.45|P-value:2.76E-4|Clearance:0.03||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:BCP1(YDR361C)|FD-Score:3.13|P-value:8.87E-4|Clearance:0||SGD DESC:Essential protein involved in nuclear export of Mss4p, which is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport Gene:BET4(YJL031C)|FD-Score:3.61|P-value:1.52E-4|Clearance:0.01||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:ENP2(YGR145W)|FD-Score:3.59|P-value:1.65E-4|Clearance:0.14||SGD DESC:Essential nucleolar protein, required for biogenesis of the small ribosomal subunit; contains WD repeats, interacts with Mpp10p and Bfr2p, and has homology to Spb1p Gene:GPI11(YDR302W)|FD-Score:3.81|P-value:7.06E-5|Clearance:0.19||SGD DESC:ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog Gene:MMS21(YEL019C)|FD-Score:3.86|P-value:5.58E-5|Clearance:0.06||SGD DESC:SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination Gene:MYO2(YOR326W)|FD-Score:-4.73|P-value:1.11E-6|Clearance:0||SGD DESC:Type V myosin motor involved in actin-based transport of cargos; required for the polarized delivery of secretory vesicles, the vacuole, late Golgi elements, peroxisomes, and the mitotic spindle; MYO2 has a paralog, MYO4, that arose from the whole genome duplication Gene:NOC2(YOR206W)|FD-Score:4.27|P-value:9.92E-6|Clearance:0.4||SGD DESC:Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors Gene:ORC3(YLL004W)|FD-Score:-3.16|P-value:8.00E-4|Clearance:0||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:PDS1(YDR113C)|FD-Score:3.2|P-value:6.92E-4|Clearance:0.07||SGD DESC:Securin, inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:PRE8(YML092C)|FD-Score:-4.37|P-value:6.27E-6|Clearance:0||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:RRN6(YBL014C)|FD-Score:3.13|P-value:8.65E-4|Clearance:0.01||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:RVB2(YPL235W)|FD-Score:4.69|P-value:1.35E-6|Clearance:0.43||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SKI6(YGR195W)|FD-Score:3.42|P-value:3.12E-4|Clearance:0.22||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4) Gene:TRE2(YOR256C)|FD-Score:3.13|P-value:8.89E-4|Clearance:0.05||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication Gene:UGP1(YKL035W)|FD-Score:-4.64|P-value:1.71E-6|Clearance:0||SGD DESC:UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication Gene:YAH1(YPL252C)|FD-Score:-4.61|P-value:2.03E-6|Clearance:0||SGD DESC:Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin Gene:YLR076C(YLR076C_d)|FD-Score:3.6|P-value:1.59E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3152970
Download HOP data (tab-delimited text)  (excel)
Gene:ABP140(YOR239W)|FD-Score:-3.63|P-value:1.41E-4||SGD DESC:AdoMet-dependent tRNA methyltransferase and actin binding protein; C-terminal domain is responsible for 3-methylcytidine modification of residue 32 of the tRNA anticodon loop of tRNA-Thr and tRNA-Ser and contains an S-adenosylmethionine (AdoMet) binding motif; N-terminal actin binding sequence interacts with actin filaments and localizes to actin patches and cables; N- and C-terminal domains are encoded in separate ORFs that are translated into one protein via a +1 frameshift Gene:ACE2(YLR131C)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:ACS1(YAL054C)|FD-Score:-3.15|P-value:8.08E-4||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs2p, is the nuclear source of acetyl-coA for histone acetylation; expressed during growth on nonfermentable carbon sources and under aerobic conditions Gene:ADK2(YER170W)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Mitochondrial adenylate kinase, catalyzes the reversible synthesis of GTP and AMP from GDP and ADP; may serve as a back-up for synthesizing GTP or ADP depending on metabolic conditions; 3' sequence of ADK2 varies with strain background Gene:AVT4(YNL101W)|FD-Score:4.14|P-value:1.74E-5||SGD DESC:Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BDS1(YOL164W)|FD-Score:-3.11|P-value:9.32E-4||SGD DESC:Bacterially-derived sulfatase required for use of alkyl- and aryl-sulfates as sulfur sources Gene:CAR2(YLR438W)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:L-ornithine transaminase (OTAse); catalyzes the second step of arginine degradation, expression is dually-regulated by allophanate induction and a specific arginine induction process; not nitrogen catabolite repression sensitive; protein abundance increases in response to DNA replication stress Gene:CPR4(YCR069W)|FD-Score:-3.18|P-value:7.32E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway Gene:CSH1(YBR161W)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication Gene:DGR1(YNL130C-A_p)|FD-Score:-3.27|P-value:5.29E-4||SGD DESC:Protein of unknown function; dgr1 null mutant is resistant to 2-deoxy-D-glucose Gene:ELA1(YNL230C)|FD-Score:3.19|P-value:7.15E-4||SGD DESC:Elongin A; F-box protein that forms a heterodimer with Elc1p and is required for ubiquitin-dependent degradation of the RNA Polymerase II subunit Rpo21p; subunit of the Elongin-Cullin-Socs (ECS) ligase complex Gene:ETR1(YBR026C)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:2-enoyl thioester reductase, member of the medium chain dehydrogenase/reductase family; localized to in mitochondria, where it has a probable role in fatty acid synthesis Gene:FEN1(YCR034W)|FD-Score:5.8|P-value:3.25E-9||SGD DESC:Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; FEN1 has a paralog, ELO1, that arose from the whole genome duplication Gene:FET4(YMR319C)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:GEP5(YLR091W)|FD-Score:3.22|P-value:6.48E-4||SGD DESC:Protein of unknown function, required for mitochondrial genome maintenance; detected in highly purified mitochondria in high-throughput studies; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine Gene:GLO2(YDR272W)|FD-Score:4.1|P-value:2.10E-5||SGD DESC:Cytoplasmic glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate Gene:GSY2(YLR258W)|FD-Score:3.97|P-value:3.58E-5||SGD DESC:Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:GTS1(YGL181W)|FD-Score:3.16|P-value:7.77E-4||SGD DESC:Protein involved in Arf3p regulation and in transcription regulation; localizes to the nucleus and to endocytic patches; contains an N-terminal Zn-finger and ArfGAP homology domain, a C-terminal glutamine-rich region, and a UBA (ubiquitin associated) domain; gts1 mutations affect budding, cell size, heat tolerance, sporulation, life span, ultradian rhythms, endocytosis; expression oscillates in a pattern similar to metabolic oscillations Gene:IRC5(YFR038W)|FD-Score:4.86|P-value:5.88E-7||SGD DESC:Putative ATPase containing the DEAD/H helicase-related sequence motif; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRS4(YKR019C)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LTV1(YKL143W)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Component of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; required for ribosomal small subunit export from nucleus; required for growth at low temperature Gene:MET10(YFR030W)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:Subunit alpha of assimilatory sulfite reductase, which converts sulfite into sulfide Gene:MPC54(YOR177C)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Component of the meiotic outer plaque, a membrane-organizing center which is assembled on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; potential Cdc28p substrate Gene:MSB1(YOR188W)|FD-Score:3.74|P-value:9.25E-5||SGD DESC:Protein of unknown function; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MSY1(YPL097W)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Mitochondrial tyrosyl-tRNA synthetase Gene:MXR1(YER042W)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Methionine-S-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR2; involved in the regulation of lifespan Gene:PFK2(YMR205C)|FD-Score:3.46|P-value:2.66E-4||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PHO80(YOL001W)|FD-Score:4.52|P-value:3.09E-6||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling Gene:PHO86(YJL117W)|FD-Score:4.11|P-value:1.99E-5||SGD DESC:Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress Gene:RIM1(YCR028C-A)|FD-Score:4.47|P-value:3.98E-6||SGD DESC:Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication Gene:RPI1(YIL119C)|FD-Score:-3.95|P-value:3.83E-5||SGD DESC:Transcription factor with allelic differences between S288C and Sigma1278b; mediates fermentation stress tolerance by modulating cell wall integrity; overexpression suppresses heat shock sensitivity of wild-type RAS2 overexpression and also suppresses cell lysis defect of mpk1 mutation; allele from S288c can confer fMAPK pathway independent transcription of FLO11; S288C and Sigma1278b alleles differ in number of tandem repeats within ORF Gene:RRT5(YFR032C_p)|FD-Score:3.26|P-value:5.57E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; expressed at high levels during sporulation Gene:SEE1(YIL064W)|FD-Score:-3.39|P-value:3.44E-4||SGD DESC:Probable lysine methyltransferase involved in the dimethylation of eEF1A (Tef1p/Tef2p); sequence similarity to S-adenosylmethionine-dependent methyltransferases of the seven beta-strand family; role in vesicular transport Gene:SIP5(YMR140W)|FD-Score:-3.41|P-value:3.24E-4||SGD DESC:Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase; forms cytoplasmic foci upon DNA replication stress Gene:SPT10(YJL127C)|FD-Score:4.07|P-value:2.39E-5||SGD DESC:Putative histone acetylase with a role in transcriptional silencing, sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box Gene:STE23(YLR389C)|FD-Score:-3.29|P-value:4.92E-4||SGD DESC:Metalloprotease involved, with homolog Axl1p, in N-terminal processing of pro-a-factor to the mature form; expressed in both haploids and diploids; member of the insulin-degrading enzyme family Gene:SWE1(YJL187C)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Protein kinase that regulates the G2/M transition by inhibition of Cdc28p kinase activity; localizes to the nucleus and to the daughter side of the mother-bud neck; homolog of S. pombe Wee1p; potential Cdc28p substrate Gene:TIF2(YJL138C)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF2 has a paralog, TIF1, that arose from the whole genome duplication Gene:TPO4(YOR273C)|FD-Score:4.45|P-value:4.32E-6||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane Gene:TRM44(YPL030W)|FD-Score:4.76|P-value:9.81E-7||SGD DESC:tRNA(Ser) Um(44) 2'-O-methyltransferase; involved in maintaining levels of the tRNA-Ser species tS(CGA) and tS(UGA); conserved among metazoans and fungi but there does not appear to be a homolog in plants; TRM44 is a non-essential gene Gene:YAR047C(YAR047C_d)|FD-Score:-3.33|P-value:4.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCL046W(YCL046W_d)|FD-Score:3.11|P-value:9.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C Gene:YDR161W(YDR161W_p)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:YDR444W(YDR444W_p)|FD-Score:4.82|P-value:7.02E-7||SGD DESC:Putative hydrolase acting on ester bonds Gene:YDR535C(YDR535C_d)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene. Gene:YGR035W-A(YGR035W-A_p)|FD-Score:4.63|P-value:1.82E-6||SGD DESC:Putative protein of unknown function Gene:YGR122W(YGR122W)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YGR259C(YGR259C_d)|FD-Score:3.14|P-value:8.47E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF TNA1/YGR260W Gene:YHI9(YHR029C)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Protein of unknown function; null mutant is defective in unfolded protein response; possibly involved in a membrane regulation metabolic pathway; member of the PhzF superfamily, though most likely not involved in phenazine production Gene:YHR095W(YHR095W_d)|FD-Score:-3.91|P-value:4.60E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL096C(YIL096C_p)|FD-Score:-3.42|P-value:3.11E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; associates with precursors of the 60S ribosomal subunit; predicted to be involved in ribosome biogenesis Gene:YKL123W(YKL123W_d)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene SSH4 Gene:YLR031W(YLR031W_p)|FD-Score:-3.22|P-value:6.45E-4||SGD DESC:Putative protein of unknown function Gene:YMD8(YML038C)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Putative nucleotide sugar transporter, has similarity to Vrg4p Gene:YMR013W-A(YMR013W-A_d)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the characterized snoRNA gene snR73 Gene:YMR265C(YMR265C_p)|FD-Score:4.73|P-value:1.13E-6||SGD DESC:Putative protein of unknown function Gene:YNL108C(YNL108C_p)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Putative protein of unknown function; similar to prokaryotic phosphotransfer enzymes; null mutant shows alterations in glucose metabolism; GFP-fusion protein localizes to the cytoplasm and nucleus; YNL108C has a paralog, TFC7, that arose from the whole genome duplication Gene:YNL226W(YNL226W_d)|FD-Score:3.8|P-value:7.24E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene JJJ1/YNL227C Gene:YOL118C(YOL118C_d)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR314W(YOR314W_d)|FD-Score:-4.45|P-value:4.34E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YSC83(YHR017W)|FD-Score:3.2|P-value:6.91E-4||SGD DESC:Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83 Gene:ABP140(YOR239W)|FD-Score:-3.63|P-value:1.41E-4||SGD DESC:AdoMet-dependent tRNA methyltransferase and actin binding protein; C-terminal domain is responsible for 3-methylcytidine modification of residue 32 of the tRNA anticodon loop of tRNA-Thr and tRNA-Ser and contains an S-adenosylmethionine (AdoMet) binding motif; N-terminal actin binding sequence interacts with actin filaments and localizes to actin patches and cables; N- and C-terminal domains are encoded in separate ORFs that are translated into one protein via a +1 frameshift Gene:ACE2(YLR131C)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:ACS1(YAL054C)|FD-Score:-3.15|P-value:8.08E-4||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs2p, is the nuclear source of acetyl-coA for histone acetylation; expressed during growth on nonfermentable carbon sources and under aerobic conditions Gene:ADK2(YER170W)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Mitochondrial adenylate kinase, catalyzes the reversible synthesis of GTP and AMP from GDP and ADP; may serve as a back-up for synthesizing GTP or ADP depending on metabolic conditions; 3' sequence of ADK2 varies with strain background Gene:AVT4(YNL101W)|FD-Score:4.14|P-value:1.74E-5||SGD DESC:Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BDS1(YOL164W)|FD-Score:-3.11|P-value:9.32E-4||SGD DESC:Bacterially-derived sulfatase required for use of alkyl- and aryl-sulfates as sulfur sources Gene:CAR2(YLR438W)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:L-ornithine transaminase (OTAse); catalyzes the second step of arginine degradation, expression is dually-regulated by allophanate induction and a specific arginine induction process; not nitrogen catabolite repression sensitive; protein abundance increases in response to DNA replication stress Gene:CPR4(YCR069W)|FD-Score:-3.18|P-value:7.32E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway Gene:CSH1(YBR161W)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication Gene:DGR1(YNL130C-A_p)|FD-Score:-3.27|P-value:5.29E-4||SGD DESC:Protein of unknown function; dgr1 null mutant is resistant to 2-deoxy-D-glucose Gene:ELA1(YNL230C)|FD-Score:3.19|P-value:7.15E-4||SGD DESC:Elongin A; F-box protein that forms a heterodimer with Elc1p and is required for ubiquitin-dependent degradation of the RNA Polymerase II subunit Rpo21p; subunit of the Elongin-Cullin-Socs (ECS) ligase complex Gene:ETR1(YBR026C)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:2-enoyl thioester reductase, member of the medium chain dehydrogenase/reductase family; localized to in mitochondria, where it has a probable role in fatty acid synthesis Gene:FEN1(YCR034W)|FD-Score:5.8|P-value:3.25E-9||SGD DESC:Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; FEN1 has a paralog, ELO1, that arose from the whole genome duplication Gene:FET4(YMR319C)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:GEP5(YLR091W)|FD-Score:3.22|P-value:6.48E-4||SGD DESC:Protein of unknown function, required for mitochondrial genome maintenance; detected in highly purified mitochondria in high-throughput studies; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine Gene:GLO2(YDR272W)|FD-Score:4.1|P-value:2.10E-5||SGD DESC:Cytoplasmic glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate Gene:GSY2(YLR258W)|FD-Score:3.97|P-value:3.58E-5||SGD DESC:Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:GTS1(YGL181W)|FD-Score:3.16|P-value:7.77E-4||SGD DESC:Protein involved in Arf3p regulation and in transcription regulation; localizes to the nucleus and to endocytic patches; contains an N-terminal Zn-finger and ArfGAP homology domain, a C-terminal glutamine-rich region, and a UBA (ubiquitin associated) domain; gts1 mutations affect budding, cell size, heat tolerance, sporulation, life span, ultradian rhythms, endocytosis; expression oscillates in a pattern similar to metabolic oscillations Gene:IRC5(YFR038W)|FD-Score:4.86|P-value:5.88E-7||SGD DESC:Putative ATPase containing the DEAD/H helicase-related sequence motif; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRS4(YKR019C)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LTV1(YKL143W)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Component of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; required for ribosomal small subunit export from nucleus; required for growth at low temperature Gene:MET10(YFR030W)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:Subunit alpha of assimilatory sulfite reductase, which converts sulfite into sulfide Gene:MPC54(YOR177C)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Component of the meiotic outer plaque, a membrane-organizing center which is assembled on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; potential Cdc28p substrate Gene:MSB1(YOR188W)|FD-Score:3.74|P-value:9.25E-5||SGD DESC:Protein of unknown function; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MSY1(YPL097W)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Mitochondrial tyrosyl-tRNA synthetase Gene:MXR1(YER042W)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Methionine-S-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR2; involved in the regulation of lifespan Gene:PFK2(YMR205C)|FD-Score:3.46|P-value:2.66E-4||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PHO80(YOL001W)|FD-Score:4.52|P-value:3.09E-6||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling Gene:PHO86(YJL117W)|FD-Score:4.11|P-value:1.99E-5||SGD DESC:Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress Gene:RIM1(YCR028C-A)|FD-Score:4.47|P-value:3.98E-6||SGD DESC:Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication Gene:RPI1(YIL119C)|FD-Score:-3.95|P-value:3.83E-5||SGD DESC:Transcription factor with allelic differences between S288C and Sigma1278b; mediates fermentation stress tolerance by modulating cell wall integrity; overexpression suppresses heat shock sensitivity of wild-type RAS2 overexpression and also suppresses cell lysis defect of mpk1 mutation; allele from S288c can confer fMAPK pathway independent transcription of FLO11; S288C and Sigma1278b alleles differ in number of tandem repeats within ORF Gene:RRT5(YFR032C_p)|FD-Score:3.26|P-value:5.57E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; expressed at high levels during sporulation Gene:SEE1(YIL064W)|FD-Score:-3.39|P-value:3.44E-4||SGD DESC:Probable lysine methyltransferase involved in the dimethylation of eEF1A (Tef1p/Tef2p); sequence similarity to S-adenosylmethionine-dependent methyltransferases of the seven beta-strand family; role in vesicular transport Gene:SIP5(YMR140W)|FD-Score:-3.41|P-value:3.24E-4||SGD DESC:Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase; forms cytoplasmic foci upon DNA replication stress Gene:SPT10(YJL127C)|FD-Score:4.07|P-value:2.39E-5||SGD DESC:Putative histone acetylase with a role in transcriptional silencing, sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box Gene:STE23(YLR389C)|FD-Score:-3.29|P-value:4.92E-4||SGD DESC:Metalloprotease involved, with homolog Axl1p, in N-terminal processing of pro-a-factor to the mature form; expressed in both haploids and diploids; member of the insulin-degrading enzyme family Gene:SWE1(YJL187C)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Protein kinase that regulates the G2/M transition by inhibition of Cdc28p kinase activity; localizes to the nucleus and to the daughter side of the mother-bud neck; homolog of S. pombe Wee1p; potential Cdc28p substrate Gene:TIF2(YJL138C)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF2 has a paralog, TIF1, that arose from the whole genome duplication Gene:TPO4(YOR273C)|FD-Score:4.45|P-value:4.32E-6||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane Gene:TRM44(YPL030W)|FD-Score:4.76|P-value:9.81E-7||SGD DESC:tRNA(Ser) Um(44) 2'-O-methyltransferase; involved in maintaining levels of the tRNA-Ser species tS(CGA) and tS(UGA); conserved among metazoans and fungi but there does not appear to be a homolog in plants; TRM44 is a non-essential gene Gene:YAR047C(YAR047C_d)|FD-Score:-3.33|P-value:4.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCL046W(YCL046W_d)|FD-Score:3.11|P-value:9.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C Gene:YDR161W(YDR161W_p)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:YDR444W(YDR444W_p)|FD-Score:4.82|P-value:7.02E-7||SGD DESC:Putative hydrolase acting on ester bonds Gene:YDR535C(YDR535C_d)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene. Gene:YGR035W-A(YGR035W-A_p)|FD-Score:4.63|P-value:1.82E-6||SGD DESC:Putative protein of unknown function Gene:YGR122W(YGR122W)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YGR259C(YGR259C_d)|FD-Score:3.14|P-value:8.47E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF TNA1/YGR260W Gene:YHI9(YHR029C)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Protein of unknown function; null mutant is defective in unfolded protein response; possibly involved in a membrane regulation metabolic pathway; member of the PhzF superfamily, though most likely not involved in phenazine production Gene:YHR095W(YHR095W_d)|FD-Score:-3.91|P-value:4.60E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL096C(YIL096C_p)|FD-Score:-3.42|P-value:3.11E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; associates with precursors of the 60S ribosomal subunit; predicted to be involved in ribosome biogenesis Gene:YKL123W(YKL123W_d)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene SSH4 Gene:YLR031W(YLR031W_p)|FD-Score:-3.22|P-value:6.45E-4||SGD DESC:Putative protein of unknown function Gene:YMD8(YML038C)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Putative nucleotide sugar transporter, has similarity to Vrg4p Gene:YMR013W-A(YMR013W-A_d)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the characterized snoRNA gene snR73 Gene:YMR265C(YMR265C_p)|FD-Score:4.73|P-value:1.13E-6||SGD DESC:Putative protein of unknown function Gene:YNL108C(YNL108C_p)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Putative protein of unknown function; similar to prokaryotic phosphotransfer enzymes; null mutant shows alterations in glucose metabolism; GFP-fusion protein localizes to the cytoplasm and nucleus; YNL108C has a paralog, TFC7, that arose from the whole genome duplication Gene:YNL226W(YNL226W_d)|FD-Score:3.8|P-value:7.24E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene JJJ1/YNL227C Gene:YOL118C(YOL118C_d)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR314W(YOR314W_d)|FD-Score:-4.45|P-value:4.34E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YSC83(YHR017W)|FD-Score:3.2|P-value:6.91E-4||SGD DESC:Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPL235W4.691.35E-60.43RVB2ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly
YOR206W4.279.92E-60.40NOC2Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors
YEL019C3.865.58E-50.06MMS21SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination
YDR302W3.817.06E-50.19GPI11ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog
YJL031C3.611.52E-40.01BET4Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p
YLR076C_d3.601.59E-40.01YLR076C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10
YGR145W3.591.65E-40.14ENP2Essential nucleolar protein, required for biogenesis of the small ribosomal subunit; contains WD repeats, interacts with Mpp10p and Bfr2p, and has homology to Spb1p
YFL039C3.452.76E-40.03ACT1Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions
YGR195W3.423.12E-40.22SKI6Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4)
YDR113C3.206.92E-40.07PDS1Securin, inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation
YBL014C3.138.65E-40.01RRN6Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
YDR361C3.138.87E-47.56E-4BCP1Essential protein involved in nuclear export of Mss4p, which is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport
YOR256C3.138.89E-40.05TRE2Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication
YBR257W3.080.001040.07POP4Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
YLR145W3.010.001320.32RMP1Subunit of RNase MRP, which processes pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; unlike most subunits, not shared between RNase MRP and nuclear RNase P

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YCR034W5.803.25E-9FEN1Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; FEN1 has a paralog, ELO1, that arose from the whole genome duplication
YFR038W4.865.88E-7IRC5Putative ATPase containing the DEAD/H helicase-related sequence motif; null mutant displays increased levels of spontaneous Rad52p foci
YDR444W_p4.827.02E-7YDR444W_pPutative hydrolase acting on ester bonds
YPL030W4.769.81E-7TRM44tRNA(Ser) Um(44) 2'-O-methyltransferase; involved in maintaining levels of the tRNA-Ser species tS(CGA) and tS(UGA); conserved among metazoans and fungi but there does not appear to be a homolog in plants; TRM44 is a non-essential gene
YMR265C_p4.731.13E-6YMR265C_pPutative protein of unknown function
YGR035W-A_p4.631.82E-6YGR035W-A_pPutative protein of unknown function
YOL001W4.523.09E-6PHO80Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling
YCR028C-A4.473.98E-6RIM1Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication
YOR273C4.454.32E-6TPO4Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane
YNL101W4.141.74E-5AVT4Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
YJL117W4.111.99E-5PHO86Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress
YDR272W4.102.10E-5GLO2Cytoplasmic glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate
YJL127C4.072.39E-5SPT10Putative histone acetylase with a role in transcriptional silencing, sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box
YLR258W3.973.58E-5GSY2Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress
YNL226W_d3.807.24E-5YNL226W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene JJJ1/YNL227C

GO enrichment analysis for SGTC_2712
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0691.16E-7SGTC_2710st078562 53.7 μMTimTec (Natural product derivative library)172509080.130435
0.0673.05E-7SGTC_9811488-1094 54.9 μMChemDiv (Drug-like library)34495220.146067
0.0656.56E-7SGTC_2657nystatin 74.4 μMMiscellaneous114687070.0756303
0.0649.57E-7SGTC_14534237-0541 195.0 μMChemDiv (Drug-like library)7398920.0843373heme biosynthesis & mitochondrial translocase
0.0613.08E-6SGTC_14033909-7815 62.9 μMChemDiv (Drug-like library)66154830.101124
0.0613.19E-6SGTC_316k060-0003 58.6 μMChemDiv (Drug-like library)61523140.0568182
0.0595.48E-6SGTC_31739105693 49.5 μMChembridge (Drug-like library)170966580.166667
0.0587.93E-6SGTC_1074carmofur 3.8 μMNIH Clinical Collection25770.126582exosome
0.0588.66E-6SGTC_33469158254 43.6 μMChembridge (Drug-like library)176395000.128205
0.0588.95E-6SGTC_1034k072-0165 12.7 μMChemDiv (Drug-like library)58354610.0238095Golgi
0.0571.12E-5SGTC_1050111-0055 734.8 μMChemDiv (Drug-like library)51793700.0571429
0.0571.13E-5SGTC_2549cedrol 20.9 μMMicrosource (Natural product library)67086650.0263158
0.0571.35E-5SGTC_1633st005664 67.9 μMTimTec (Natural product derivative library)20638220.121622
0.0561.60E-5SGTC_29429052777 62.2 μMChembridge (Drug-like library)64695030.0897436
0.0561.62E-5SGTC_12380333-0287 48.8 μMChemDiv (Drug-like library)72260.0410959Golgi

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2740felodipine17.08 μM0.3428573333Miscellaneous384.253763.54915
SGTC_488nitrendipine139 μM0.3424664507Miscellaneous360.36122.11517
SGTC_1080nisoldipine24.4 μM0.3378384499Miscellaneous388.414362.95817TSC3-RPN4
SGTC_481bay k-864450 μM0.3287672303ICCB bioactive library356.296512.67318
SGTC_1707st0323236.32 μM0.3066673132057TimTec (Natural product derivative library)436.258162.82624DNA damage response
SGTC_486niguldipine82 μM0.2857141236Miscellaneous609.711365.40718amide catabolism
SGTC_1662st01405452 μM0.2752838777TimTec (Natural product derivative library)360.362742.92816TSC3-RPN4
SGTC_1822st05246842 μM0.2717392873678TimTec (Natural product derivative library)475.920283.87516
SGTC_487nimodipine119 μM0.2682934497Miscellaneous418.440342.36118
SGTC_3620310-01009.03 μM0.267606790235ChemDiv (Drug-like library)279.246962.8605RPP1 & pyrimidine depletion