st080858

4-[(Z)-2-(9H-pyrido[3,4-b]indol-1-yl)ethenyl]benzonitrile

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2716
Screen concentration 38.6 μM
Source TimTec (Natural product derivative library)
PubChem CID 5296050
SMILES C1=CC=C2C(=C1)C3=C(N2)C(=NC=C3)C=CC4=CC=C(C=C4)C#N
Standardized SMILES N#Cc1ccc(C=Cc2[nH]ccc3c4ccccc4nc23)cc1
Molecular weight 295.3373
ALogP 4.47
H-bond donor count 1
H-bond acceptor count 2
Response signature 60S ribosome export

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.24
% growth inhibition (Hom. pool) 4.61


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5296050
Download HIP data (tab-delimited text)  (excel)
Gene:CDC36(YDL165W)|FD-Score:4.87|P-value:5.68E-7|Clearance:1.26||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:DIS3(YOL021C)|FD-Score:-4.43|P-value:4.71E-6|Clearance:0||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:GLC7(YER133W)|FD-Score:3.23|P-value:6.22E-4|Clearance:0.02||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:LSG1(YGL099W)|FD-Score:10.2|P-value:1.33E-24|Clearance:5.31||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MET4(YNL103W)|FD-Score:3.21|P-value:6.72E-4|Clearance:0.05||SGD DESC:Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p Gene:NMD3(YHR170W)|FD-Score:3.49|P-value:2.41E-4|Clearance:0.26||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:POL31(YJR006W)|FD-Score:3.21|P-value:6.61E-4|Clearance:0||SGD DESC:Subunit of DNA polymerase delta (polymerase III); essential for cell viability; involved in DNA replication and DNA repair Gene:PRI2(YKL045W)|FD-Score:3.15|P-value:8.05E-4|Clearance:0.12||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:PSE1(YMR308C)|FD-Score:3.61|P-value:1.55E-4|Clearance:0.12||SGD DESC:Karyopherin/importin that interacts with the nuclear pore complex; acts as the nuclear import receptor for specific proteins, including Pdr1p, Yap1p, Ste12p, and Aft1p Gene:SEC31(YDL195W)|FD-Score:-6.72|P-value:9.24E-12|Clearance:0||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:UTP6(YDR449C)|FD-Score:-3.92|P-value:4.42E-5|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:CDC36(YDL165W)|FD-Score:4.87|P-value:5.68E-7|Clearance:1.26||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:DIS3(YOL021C)|FD-Score:-4.43|P-value:4.71E-6|Clearance:0||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:GLC7(YER133W)|FD-Score:3.23|P-value:6.22E-4|Clearance:0.02||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:LSG1(YGL099W)|FD-Score:10.2|P-value:1.33E-24|Clearance:5.31||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MET4(YNL103W)|FD-Score:3.21|P-value:6.72E-4|Clearance:0.05||SGD DESC:Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p Gene:NMD3(YHR170W)|FD-Score:3.49|P-value:2.41E-4|Clearance:0.26||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:POL31(YJR006W)|FD-Score:3.21|P-value:6.61E-4|Clearance:0||SGD DESC:Subunit of DNA polymerase delta (polymerase III); essential for cell viability; involved in DNA replication and DNA repair Gene:PRI2(YKL045W)|FD-Score:3.15|P-value:8.05E-4|Clearance:0.12||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:PSE1(YMR308C)|FD-Score:3.61|P-value:1.55E-4|Clearance:0.12||SGD DESC:Karyopherin/importin that interacts with the nuclear pore complex; acts as the nuclear import receptor for specific proteins, including Pdr1p, Yap1p, Ste12p, and Aft1p Gene:SEC31(YDL195W)|FD-Score:-6.72|P-value:9.24E-12|Clearance:0||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:UTP6(YDR449C)|FD-Score:-3.92|P-value:4.42E-5|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5296050
Download HOP data (tab-delimited text)  (excel)
Gene:AGC1(YPR021C)|FD-Score:4.33|P-value:7.53E-6||SGD DESC:Mitochondrial amino acid transporter, acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism and nitrogen compound biosynthesis Gene:AIM21(YIR003W)|FD-Score:3.3|P-value:4.84E-4||SGD DESC:Protein of unknown function involved in mitochondrial migration along actin filament; may interact with ribosomes; GFP-fusion protein colocalizes with Sac1p to the actin cytoskeleton Gene:ALY2(YJL084C)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:Alpha arrestin that controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; phosphorylated by Npr1p and also by cyclin-CDK complex Pcl7p-Pho85p; promotes endocytosis of plasma membrane proteins; ALY2 has a paralog, ALY1, that arose from the whole genome duplication Gene:ARP8(YOR141C)|FD-Score:5.04|P-value:2.30E-7||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes; has mRNA binding activity Gene:AVT2(YEL064C)|FD-Score:-3.24|P-value:5.89E-4||SGD DESC:Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BEM4(YPL161C)|FD-Score:5.77|P-value:3.96E-9||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BUD17(YNR027W)|FD-Score:-3.35|P-value:4.05E-4||SGD DESC:Putative pyridoxal kinase, a key enzyme in vitamin B6 metabolism; involved in bud-site selection; diploid mutants display a random rather than a bipolar budding pattern; similarity to yeast BUD16 and human pyridoxal kinase (PDXK) Gene:CBP4(YGR174C)|FD-Score:3.98|P-value:3.51E-5||SGD DESC:Mitochondrial protein required for assembly of cytochrome bc1 complex; interacts with the Cbp3p-Cbp6p complex and newly synthesized cytochrome b (Cobp) to promote assembly of Cobp into the cytochrome bc1 complex Gene:COG8(YML071C)|FD-Score:3.11|P-value:9.27E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CSI1(YMR025W)|FD-Score:4.95|P-value:3.64E-7||SGD DESC:Subunit of the Cop9 signalosome, which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling; functional equivalent of canonical Csn6 subunit of the COP9 signalosome Gene:DDP1(YOR163W)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:Polyphosphate phosphatase; hydrolyzes diphosphorylated inositol polyphosphates and diadenosine polyphosphates; high specificity for diadenosine hexa- and pentaphosphates; contains endopolyphosphatase activity with a high affinity for polyphosphates, an activity also observed for its human DIPP homologs; possesses mRNA decapping activity; nudix hydrolase family member; protein abundance increases in response to DNA replication stress Gene:DGA1(YOR245C)|FD-Score:3.62|P-value:1.44E-4||SGD DESC:Diacylglycerol acyltransferase, catalyzes the terminal step of triacylglycerol (TAG) formation, acylates diacylglycerol using acyl-CoA as an acyl donor, localized to lipid particles Gene:DLD3(YEL071W)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:D-lactate dehydrogenase, part of the retrograde regulon which consists of genes whose expression is stimulated by damage to mitochondria and reduced in cells grown with glutamate as the sole nitrogen source, located in the cytoplasm Gene:ECM27(YJR106W)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants are hypersensitive to Papulacandin B; null mutants have increased plasmid loss; displays a two-hybrid interaction with Pdr5p Gene:EMC4(YGL231C)|FD-Score:-3.43|P-value:3.04E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4, fly CG11137, human TMM85 Gene:EUG1(YDR518W)|FD-Score:3.17|P-value:7.56E-4||SGD DESC:Protein disulfide isomerase of the endoplasmic reticulum lumen; EUG1 has a paralog, PDI1, that arose from the whole genome duplication; function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER Gene:FAR10(YLR238W)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far11p; potential Cdc28p substrate Gene:GEA1(YJR031C)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication Gene:HKR1(YDR420W)|FD-Score:-3.7|P-value:1.10E-4||SGD DESC:Mucin family member that functions as an osmosensor in the Sho1p-mediated HOG pathway with Msb2p; proposed to be a negative regulator of filamentous growth; mutant displays defects in beta-1,3 glucan synthesis and bud site selection Gene:HOG1(YLR113W)|FD-Score:-3.34|P-value:4.16E-4||SGD DESC:Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNA Pol II in response to osmotic shock; acts via three independent osmosensors; mitophagy-specific regulator; mediates the recruitment and activation of RNA Pol II at Hot1p-dependent promoters; localization regulated by Ptp2p and Ptp3p; protein abundance increases in response to DNA replication stress Gene:INP51(YIL002C)|FD-Score:4.06|P-value:2.45E-5||SGD DESC:Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth Gene:IRS4(YKR019C)|FD-Score:6.31|P-value:1.41E-10||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:IXR1(YKL032C)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (high mobility group box) domains, which confer the ability to bend cisplatin-modified DNA; mediates aerobic transcriptional repression of COX5b Gene:IZH3(YLR023C)|FD-Score:3.98|P-value:3.50E-5||SGD DESC:Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family, expression induced by zinc deficiency; deletion reduces sensitivity to elevated zinc and shortens lag phase, overexpression reduces Zap1p activity Gene:JLP1(YLL057C)|FD-Score:3.16|P-value:7.96E-4||SGD DESC:Fe(II)-dependent sulfonate/alpha-ketoglutarate dioxygenase, involved in sulfonate catabolism for use as a sulfur source; contains sequence that resembles a J domain (typified by the E. coli DnaJ protein); induced by sulphur starvation Gene:MCA1(YOR197W)|FD-Score:3.98|P-value:3.46E-5||SGD DESC:Ca2+-dependent cysteine protease; may cleave specific substrates during the stress response; regulates apoptosis upon H2O2 treatment; required for clearance of insoluble protein aggregates during normal growth; implicated in cell cycle dynamics; undergoes autocatalytic processing; similar to mammalian metacaspases, but exists as a monomer due to an extra pair of anti-parallel beta-strands that form a continuous beta-sheet, blocking potential dimerization Gene:MRPL28(YDR462W)|FD-Score:-3.4|P-value:3.38E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MRPL49(YJL096W)|FD-Score:-4.16|P-value:1.61E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:OM14(YBR230C)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:Integral mitochondrial outer membrane protein; abundance is decreased in cells grown in glucose relative to other carbon sources; appears to contain 3 alpha-helical transmembrane segments; ORF encodes a 97-basepair intron Gene:OSM1(YJR051W)|FD-Score:4.03|P-value:2.74E-5||SGD DESC:Fumarate reductase, catalyzes the reduction of fumarate to succinate; required for the reoxidation of intracellular NADH under anaerobic conditions; mutations cause osmotic sensitivity; OSM1 has a paralog, FRD1, that arose from the whole genome duplication Gene:PDR1(YGL013C)|FD-Score:4.37|P-value:6.27E-6||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PML39(YML107C)|FD-Score:4.2|P-value:1.33E-5||SGD DESC:Protein required for nuclear retention of unspliced pre-mRNAs along with Mlp1p and Pml1p; anchored to nuclear pore complex via Mlp1p and Mlp2p; found with the subset of nuclear pores farthest from the nucleolus; may interact with ribosomes Gene:PMS1(YNL082W)|FD-Score:4.21|P-value:1.25E-5||SGD DESC:ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL Gene:RIC1(YLR039C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:ROG3(YFR022W)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Protein that binds the ubiquitin ligase Rsp5p via its 2 PY motifs; mutation suppresses the temperature sensitivity of an mck1 rim11 double mutant; proposed to regulate the endocytosis of plasma membrane proteins; ROG3 has a paralog, ROD1, that arose from the whole genome duplication Gene:RPL20A(YMR242C)|FD-Score:3.79|P-value:7.48E-5||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RTC1(YOL138C)|FD-Score:-3.33|P-value:4.27E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; null mutation suppresses cdc13-1 temperature sensitivity; has N-terminal WD-40 repeats and a C-terminal RING motif Gene:SER2(YGR208W)|FD-Score:5.86|P-value:2.35E-9||SGD DESC:Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source Gene:SFB2(YNL049C)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Component of the Sec23p-Sfb2p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb3p Gene:SLS1(YLR139C)|FD-Score:-3.2|P-value:6.96E-4||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SLX9(YGR081C)|FD-Score:-3.75|P-value:8.70E-5||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SMY1(YKL079W)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:Protein that interacts with Myo2p, proposed to be involved in exocytosis; N-terminal domain is related to the motor domain of kinesins Gene:SSD1(YDR293C)|FD-Score:5.11|P-value:1.65E-7||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:STP22(YCL008C)|FD-Score:3.1|P-value:9.83E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:SUL2(YLR092W)|FD-Score:3.45|P-value:2.84E-4||SGD DESC:High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates Gene:THI72(YOR192C)|FD-Score:3.93|P-value:4.26E-5||SGD DESC:Transporter of thiamine or related compound; shares sequence similarity with Thi7p Gene:TRM3(YDL112W)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:2'-O-ribose methyltransferase, catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs Gene:TRM44(YPL030W)|FD-Score:-3.11|P-value:9.50E-4||SGD DESC:tRNA(Ser) Um(44) 2'-O-methyltransferase; involved in maintaining levels of the tRNA-Ser species tS(CGA) and tS(UGA); conserved among metazoans and fungi but there does not appear to be a homolog in plants; TRM44 is a non-essential gene Gene:TRM82(YDR165W)|FD-Score:-3.29|P-value:5.03E-4||SGD DESC:Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p Gene:TSC3(YBR058C-A)|FD-Score:4.79|P-value:8.17E-7||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VMA3(YEL027W)|FD-Score:-3.42|P-value:3.17E-4||SGD DESC:Proteolipid subunit of the vacuolar H(+)-ATPase V0 sector (subunit c; dicyclohexylcarbodiimide binding subunit); required for vacuolar acidification and important for copper and iron metal ion homeostasis Gene:VPS20(YMR077C)|FD-Score:-3.85|P-value:5.93E-5||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:YBR085C-A(YBR085C-A_p)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus; protein abundance increases in response to DNA replication stress Gene:YBR224W(YBR224W_d)|FD-Score:-4.05|P-value:2.51E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene TDP1 Gene:YDL041W(YDL041W_d)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YER077C(YER077C_p)|FD-Score:-3.64|P-value:1.34E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport Gene:YJL118W(YJL118W)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; YJL18W is a non-essential gene; deletion enhances the toxicity of heterologously expressed human alpha-synuclein Gene:YJL160C(YJL160C_p)|FD-Score:-3.37|P-value:3.76E-4||SGD DESC:Putative protein of unknown function; member of the PIR (proteins with internal repeats) family of cell wall proteins; non-essential gene that is required for sporulation; mRNA is weakly cell cycle regulated, peaking in mitosis; YJL160C has a paralog, PIR1, that arose from the whole genome duplication Gene:YLL047W(YLL047W_d)|FD-Score:-3.9|P-value:4.80E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RNP1 Gene:YLR030W(YLR030W_p)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Putative protein of unknown function Gene:YLR124W(YLR124W_d)|FD-Score:4.83|P-value:6.86E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR264C-A(YLR264C-A_p)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:Putative protein of unknown function Gene:YMR031W-A(YMR031W-A_d)|FD-Score:-3.31|P-value:4.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C Gene:YNL235C(YNL235C_d)|FD-Score:3.16|P-value:7.89E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex Gene:YOL079W(YOL079W_d)|FD-Score:3.09|P-value:9.98E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR223W(YOR223W)|FD-Score:4.94|P-value:3.82E-7||SGD DESC:Subunit of the DSC ubiquitin ligase complex; protein of unknown function that localizes to the ER and vacuole lumen; overexpression affects endocytic protein trafficking; ortholog of fission yeast dsc3 Gene:YOR292C(YOR292C_p)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YOR292C is not an essential gene Gene:AGC1(YPR021C)|FD-Score:4.33|P-value:7.53E-6||SGD DESC:Mitochondrial amino acid transporter, acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism and nitrogen compound biosynthesis Gene:AIM21(YIR003W)|FD-Score:3.3|P-value:4.84E-4||SGD DESC:Protein of unknown function involved in mitochondrial migration along actin filament; may interact with ribosomes; GFP-fusion protein colocalizes with Sac1p to the actin cytoskeleton Gene:ALY2(YJL084C)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:Alpha arrestin that controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; phosphorylated by Npr1p and also by cyclin-CDK complex Pcl7p-Pho85p; promotes endocytosis of plasma membrane proteins; ALY2 has a paralog, ALY1, that arose from the whole genome duplication Gene:ARP8(YOR141C)|FD-Score:5.04|P-value:2.30E-7||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes; has mRNA binding activity Gene:AVT2(YEL064C)|FD-Score:-3.24|P-value:5.89E-4||SGD DESC:Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BEM4(YPL161C)|FD-Score:5.77|P-value:3.96E-9||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BUD17(YNR027W)|FD-Score:-3.35|P-value:4.05E-4||SGD DESC:Putative pyridoxal kinase, a key enzyme in vitamin B6 metabolism; involved in bud-site selection; diploid mutants display a random rather than a bipolar budding pattern; similarity to yeast BUD16 and human pyridoxal kinase (PDXK) Gene:CBP4(YGR174C)|FD-Score:3.98|P-value:3.51E-5||SGD DESC:Mitochondrial protein required for assembly of cytochrome bc1 complex; interacts with the Cbp3p-Cbp6p complex and newly synthesized cytochrome b (Cobp) to promote assembly of Cobp into the cytochrome bc1 complex Gene:COG8(YML071C)|FD-Score:3.11|P-value:9.27E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CSI1(YMR025W)|FD-Score:4.95|P-value:3.64E-7||SGD DESC:Subunit of the Cop9 signalosome, which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling; functional equivalent of canonical Csn6 subunit of the COP9 signalosome Gene:DDP1(YOR163W)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:Polyphosphate phosphatase; hydrolyzes diphosphorylated inositol polyphosphates and diadenosine polyphosphates; high specificity for diadenosine hexa- and pentaphosphates; contains endopolyphosphatase activity with a high affinity for polyphosphates, an activity also observed for its human DIPP homologs; possesses mRNA decapping activity; nudix hydrolase family member; protein abundance increases in response to DNA replication stress Gene:DGA1(YOR245C)|FD-Score:3.62|P-value:1.44E-4||SGD DESC:Diacylglycerol acyltransferase, catalyzes the terminal step of triacylglycerol (TAG) formation, acylates diacylglycerol using acyl-CoA as an acyl donor, localized to lipid particles Gene:DLD3(YEL071W)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:D-lactate dehydrogenase, part of the retrograde regulon which consists of genes whose expression is stimulated by damage to mitochondria and reduced in cells grown with glutamate as the sole nitrogen source, located in the cytoplasm Gene:ECM27(YJR106W)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants are hypersensitive to Papulacandin B; null mutants have increased plasmid loss; displays a two-hybrid interaction with Pdr5p Gene:EMC4(YGL231C)|FD-Score:-3.43|P-value:3.04E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4, fly CG11137, human TMM85 Gene:EUG1(YDR518W)|FD-Score:3.17|P-value:7.56E-4||SGD DESC:Protein disulfide isomerase of the endoplasmic reticulum lumen; EUG1 has a paralog, PDI1, that arose from the whole genome duplication; function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER Gene:FAR10(YLR238W)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far11p; potential Cdc28p substrate Gene:GEA1(YJR031C)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication Gene:HKR1(YDR420W)|FD-Score:-3.7|P-value:1.10E-4||SGD DESC:Mucin family member that functions as an osmosensor in the Sho1p-mediated HOG pathway with Msb2p; proposed to be a negative regulator of filamentous growth; mutant displays defects in beta-1,3 glucan synthesis and bud site selection Gene:HOG1(YLR113W)|FD-Score:-3.34|P-value:4.16E-4||SGD DESC:Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNA Pol II in response to osmotic shock; acts via three independent osmosensors; mitophagy-specific regulator; mediates the recruitment and activation of RNA Pol II at Hot1p-dependent promoters; localization regulated by Ptp2p and Ptp3p; protein abundance increases in response to DNA replication stress Gene:INP51(YIL002C)|FD-Score:4.06|P-value:2.45E-5||SGD DESC:Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth Gene:IRS4(YKR019C)|FD-Score:6.31|P-value:1.41E-10||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:IXR1(YKL032C)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (high mobility group box) domains, which confer the ability to bend cisplatin-modified DNA; mediates aerobic transcriptional repression of COX5b Gene:IZH3(YLR023C)|FD-Score:3.98|P-value:3.50E-5||SGD DESC:Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family, expression induced by zinc deficiency; deletion reduces sensitivity to elevated zinc and shortens lag phase, overexpression reduces Zap1p activity Gene:JLP1(YLL057C)|FD-Score:3.16|P-value:7.96E-4||SGD DESC:Fe(II)-dependent sulfonate/alpha-ketoglutarate dioxygenase, involved in sulfonate catabolism for use as a sulfur source; contains sequence that resembles a J domain (typified by the E. coli DnaJ protein); induced by sulphur starvation Gene:MCA1(YOR197W)|FD-Score:3.98|P-value:3.46E-5||SGD DESC:Ca2+-dependent cysteine protease; may cleave specific substrates during the stress response; regulates apoptosis upon H2O2 treatment; required for clearance of insoluble protein aggregates during normal growth; implicated in cell cycle dynamics; undergoes autocatalytic processing; similar to mammalian metacaspases, but exists as a monomer due to an extra pair of anti-parallel beta-strands that form a continuous beta-sheet, blocking potential dimerization Gene:MRPL28(YDR462W)|FD-Score:-3.4|P-value:3.38E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MRPL49(YJL096W)|FD-Score:-4.16|P-value:1.61E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:OM14(YBR230C)|FD-Score:3.3|P-value:4.83E-4||SGD DESC:Integral mitochondrial outer membrane protein; abundance is decreased in cells grown in glucose relative to other carbon sources; appears to contain 3 alpha-helical transmembrane segments; ORF encodes a 97-basepair intron Gene:OSM1(YJR051W)|FD-Score:4.03|P-value:2.74E-5||SGD DESC:Fumarate reductase, catalyzes the reduction of fumarate to succinate; required for the reoxidation of intracellular NADH under anaerobic conditions; mutations cause osmotic sensitivity; OSM1 has a paralog, FRD1, that arose from the whole genome duplication Gene:PDR1(YGL013C)|FD-Score:4.37|P-value:6.27E-6||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PML39(YML107C)|FD-Score:4.2|P-value:1.33E-5||SGD DESC:Protein required for nuclear retention of unspliced pre-mRNAs along with Mlp1p and Pml1p; anchored to nuclear pore complex via Mlp1p and Mlp2p; found with the subset of nuclear pores farthest from the nucleolus; may interact with ribosomes Gene:PMS1(YNL082W)|FD-Score:4.21|P-value:1.25E-5||SGD DESC:ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL Gene:RIC1(YLR039C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:ROG3(YFR022W)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Protein that binds the ubiquitin ligase Rsp5p via its 2 PY motifs; mutation suppresses the temperature sensitivity of an mck1 rim11 double mutant; proposed to regulate the endocytosis of plasma membrane proteins; ROG3 has a paralog, ROD1, that arose from the whole genome duplication Gene:RPL20A(YMR242C)|FD-Score:3.79|P-value:7.48E-5||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RTC1(YOL138C)|FD-Score:-3.33|P-value:4.27E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; null mutation suppresses cdc13-1 temperature sensitivity; has N-terminal WD-40 repeats and a C-terminal RING motif Gene:SER2(YGR208W)|FD-Score:5.86|P-value:2.35E-9||SGD DESC:Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source Gene:SFB2(YNL049C)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Component of the Sec23p-Sfb2p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb3p Gene:SLS1(YLR139C)|FD-Score:-3.2|P-value:6.96E-4||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SLX9(YGR081C)|FD-Score:-3.75|P-value:8.70E-5||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SMY1(YKL079W)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:Protein that interacts with Myo2p, proposed to be involved in exocytosis; N-terminal domain is related to the motor domain of kinesins Gene:SSD1(YDR293C)|FD-Score:5.11|P-value:1.65E-7||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:STP22(YCL008C)|FD-Score:3.1|P-value:9.83E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:SUL2(YLR092W)|FD-Score:3.45|P-value:2.84E-4||SGD DESC:High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates Gene:THI72(YOR192C)|FD-Score:3.93|P-value:4.26E-5||SGD DESC:Transporter of thiamine or related compound; shares sequence similarity with Thi7p Gene:TRM3(YDL112W)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:2'-O-ribose methyltransferase, catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs Gene:TRM44(YPL030W)|FD-Score:-3.11|P-value:9.50E-4||SGD DESC:tRNA(Ser) Um(44) 2'-O-methyltransferase; involved in maintaining levels of the tRNA-Ser species tS(CGA) and tS(UGA); conserved among metazoans and fungi but there does not appear to be a homolog in plants; TRM44 is a non-essential gene Gene:TRM82(YDR165W)|FD-Score:-3.29|P-value:5.03E-4||SGD DESC:Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p Gene:TSC3(YBR058C-A)|FD-Score:4.79|P-value:8.17E-7||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VMA3(YEL027W)|FD-Score:-3.42|P-value:3.17E-4||SGD DESC:Proteolipid subunit of the vacuolar H(+)-ATPase V0 sector (subunit c; dicyclohexylcarbodiimide binding subunit); required for vacuolar acidification and important for copper and iron metal ion homeostasis Gene:VPS20(YMR077C)|FD-Score:-3.85|P-value:5.93E-5||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:YBR085C-A(YBR085C-A_p)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus; protein abundance increases in response to DNA replication stress Gene:YBR224W(YBR224W_d)|FD-Score:-4.05|P-value:2.51E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene TDP1 Gene:YDL041W(YDL041W_d)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YER077C(YER077C_p)|FD-Score:-3.64|P-value:1.34E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport Gene:YJL118W(YJL118W)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; YJL18W is a non-essential gene; deletion enhances the toxicity of heterologously expressed human alpha-synuclein Gene:YJL160C(YJL160C_p)|FD-Score:-3.37|P-value:3.76E-4||SGD DESC:Putative protein of unknown function; member of the PIR (proteins with internal repeats) family of cell wall proteins; non-essential gene that is required for sporulation; mRNA is weakly cell cycle regulated, peaking in mitosis; YJL160C has a paralog, PIR1, that arose from the whole genome duplication Gene:YLL047W(YLL047W_d)|FD-Score:-3.9|P-value:4.80E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RNP1 Gene:YLR030W(YLR030W_p)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Putative protein of unknown function Gene:YLR124W(YLR124W_d)|FD-Score:4.83|P-value:6.86E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR264C-A(YLR264C-A_p)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:Putative protein of unknown function Gene:YMR031W-A(YMR031W-A_d)|FD-Score:-3.31|P-value:4.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C Gene:YNL235C(YNL235C_d)|FD-Score:3.16|P-value:7.89E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex Gene:YOL079W(YOL079W_d)|FD-Score:3.09|P-value:9.98E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR223W(YOR223W)|FD-Score:4.94|P-value:3.82E-7||SGD DESC:Subunit of the DSC ubiquitin ligase complex; protein of unknown function that localizes to the ER and vacuole lumen; overexpression affects endocytic protein trafficking; ortholog of fission yeast dsc3 Gene:YOR292C(YOR292C_p)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YOR292C is not an essential gene

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL099W10.201.33E-245.31LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YDL165W4.875.68E-71.26CDC36Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor
YMR308C3.611.55E-40.12PSE1Karyopherin/importin that interacts with the nuclear pore complex; acts as the nuclear import receptor for specific proteins, including Pdr1p, Yap1p, Ste12p, and Aft1p
YHR170W3.492.41E-40.26NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YER133W3.236.22E-40.02GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YJR006W3.216.61E-40.00POL31Subunit of DNA polymerase delta (polymerase III); essential for cell viability; involved in DNA replication and DNA repair
YNL103W3.216.72E-40.05MET4Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p
YKL045W3.158.05E-40.12PRI2Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair
YNL137C3.030.001210.16NAM9Mitochondrial ribosomal component of the small subunit
YPL093W2.870.002050.02NOG1Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins
YKL153W_d2.850.002200.03YKL153W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant
YLR359W2.810.002440.00ADE13Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency
YER148W2.810.002470.02SPT15TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability
YER172C2.790.002650.04BRR2RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis; homologous to human U5-200kD
YKL082C2.750.002995.24E-4RRP14Essential protein, constituent of 66S pre-ribosomal particles; interacts with proteins involved in ribosomal biogenesis and cell polarity; member of the SURF-6 family

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKR019C6.311.41E-10IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
YGR208W5.862.35E-9SER2Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source
YPL161C5.773.96E-9BEM4Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length
YDR293C5.111.65E-7SSD1Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function
YOR141C5.042.30E-7ARP8Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes; has mRNA binding activity
YMR025W4.953.64E-7CSI1Subunit of the Cop9 signalosome, which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling; functional equivalent of canonical Csn6 subunit of the COP9 signalosome
YOR223W4.943.82E-7YOR223WSubunit of the DSC ubiquitin ligase complex; protein of unknown function that localizes to the ER and vacuole lumen; overexpression affects endocytic protein trafficking; ortholog of fission yeast dsc3
YLR124W_d4.836.86E-7YLR124W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YBR058C-A4.798.17E-7TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YGL013C4.376.27E-6PDR1Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication
YPR021C4.337.53E-6AGC1Mitochondrial amino acid transporter, acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism and nitrogen compound biosynthesis
YLR264C-A_p4.261.04E-5YLR264C-A_pPutative protein of unknown function
YLR030W_p4.241.12E-5YLR030W_pPutative protein of unknown function
YEL071W4.221.24E-5DLD3D-lactate dehydrogenase, part of the retrograde regulon which consists of genes whose expression is stimulated by damage to mitochondria and reduced in cells grown with glutamate as the sole nitrogen source, located in the cytoplasm
YNL082W4.211.25E-5PMS1ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL

GO enrichment analysis for SGTC_2716
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2171.10E-63SGTC_618k072-0232 33.2 μMChemDiv (Drug-like library)58727560.14705960S ribosome export
0.1611.54E-35SGTC_4253825-7017 13.1 μMChemDiv (Drug-like library)33974400.109589
0.1598.24E-35SGTC_1070indatraline 8.8 μMNIH Clinical Collection103144720.073529460S ribosome export
0.1591.30E-34SGTC_970535-0701 19.1 μMChemDiv (Drug-like library)28320130.078947460S ribosome export
0.1526.54E-32SGTC_14944358-1428 177.0 μMChemDiv (Drug-like library)15645710.0588235
0.1512.24E-31SGTC_2740felodipine 17.1 μMMiscellaneous33330.0506329
0.1507.05E-31SGTC_7651319-0117 244.0 μMChemDiv (Drug-like library)34877460.11363660S ribosome export
0.1491.51E-30SGTC_1775st081588 35.2 μMTimTec (Natural product derivative library)14264430.034482860S ribosome export
0.1453.71E-29SGTC_850868-0259 160.1 μMChemDiv (Drug-like library)831730.074626960S ribosome export
0.1391.29E-26SGTC_22067254556 147.0 μMChembridge (Fragment library)43989050.070422560S ribosome export
0.1382.17E-26SGTC_1700st030840 7.9 μMTimTec (Natural product derivative library)54993070.0430108PDR1
0.1351.68E-25SGTC_14033909-7815 62.9 μMChemDiv (Drug-like library)66154830.101266
0.1321.63E-24SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.196721TSC3-RPN4
0.1321.70E-24SGTC_8103825-7150 143.0 μMChemDiv (Drug-like library)223304110.10256460S ribosome export
0.1322.54E-24SGTC_1971st069429 63.5 μMTimTec (Natural product derivative library)12465220.093333360S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1721st0821529.27 μM0.5849065296051TimTec (Natural product derivative library)314.337364.3551360S ribosome export
SGTC_1516st013856110 μM0.4565225281404TimTec (Pure natural product library)182.22122.45511
SGTC_1547harmine94.2 μM0.2807025280953Miscellaneous212.247182.43812ergosterol biosynthesis
SGTC_2525harmine47.85 μM0.2807025280953Miscellaneous212.247182.43812ergosterol biosynthesis
SGTC_2366907044160.58 μM0.2692316837235Chembridge (Fragment library)197.232541.09912
SGTC_6861319-0111510 μM0.2307694562600ChemDiv (Drug-like library)392.4094451560S ribosome export
SGTC_9531188-00459.7 μM0.228076144426ChemDiv (Drug-like library)245.31844.9170160S ribosome export
SGTC_6890833-003998 μM0.224495374726ChemDiv (Drug-like library)195.259723.15101ergosterol depletion effects on membrane
SGTC_1350933-000426.35 μM0.21538521230617ChemDiv (Drug-like library)235.240762.9721360S ribosome export
SGTC_5221273-001011.6 μM0.2153855705015ChemDiv (Drug-like library)265.266743.6321360S ribosome export
SGTC_1953st07680012.1 μM0.214286297591TimTec (Natural product derivative library)219.281124.53410