fluspirilene FDA approved compound

8-[4,4-bis(4-fluorophenyl)butyl]-1-phenyl-1,3,8-triazaspiro[4.5]decan-4-one

An atypical antipsychotic.

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 

PubChem MeSH terms: Antipsychotic Agents;Dopamine Antagonists



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2720
Screen concentration 36.9 μM
Source Miscellaneous
PubChem CID 3396
SMILES C1CN(CCC12C(=O)NCN2C3=CC=CC=C3)CCCC(C4=CC=C(C=C4)F)C5=CC=C(C=C5)F
Standardized SMILES Fc1ccc(cc1)C(CCCN2CCC3(CC2)N(CNC3=O)c4ccccc4)c5ccc(F)cc5
Molecular weight 475.5727
ALogP 5.55
H-bond donor count 1
H-bond acceptor count 5
Response signature ERG2

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.38
% growth inhibition (Hom. pool) 6.28


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3396
Download HIP data (tab-delimited text)  (excel)
Gene:BET1(YIL004C)|FD-Score:3.97|P-value:3.57E-5|Clearance:0.41||SGD DESC:Type II membrane protein required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins Gene:CCT7(YJL111W)|FD-Score:4.66|P-value:1.55E-6|Clearance:0.41||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CSE1(YGL238W)|FD-Score:3.16|P-value:7.76E-4|Clearance:0||SGD DESC:Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation Gene:ERG29(YMR134W_p)|FD-Score:-5.17|P-value:1.16E-7|Clearance:0||SGD DESC:Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes Gene:RPP1(YHR062C)|FD-Score:4.35|P-value:6.85E-6|Clearance:0.41||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRP9(YPR137W)|FD-Score:3.16|P-value:7.86E-4|Clearance:0.14||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:SEC27(YGL137W)|FD-Score:3.68|P-value:1.15E-4|Clearance:0.41||SGD DESC:Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP Gene:SPP2(YOR148C)|FD-Score:3.27|P-value:5.39E-4|Clearance:0.1||SGD DESC:Essential protein that promotes the first step of splicing and is required for the final stages of spliceosome maturation; interacts with Prp2p, which may release Spp2p from the spliceosome following the first cleavage reaction Gene:SRP14(YDL092W)|FD-Score:-4.15|P-value:1.66E-5|Clearance:0||SGD DESC:Signal recognition particle (SRP) subunit, interacts with the RNA component of SRP to form the Alu domain, which is the region of SRP responsible for arrest of nascent chain elongation during membrane targeting; homolog of mammalian SRP14 Gene:TIF11(YMR260C)|FD-Score:4.79|P-value:8.41E-7|Clearance:0.41||SGD DESC:Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3 Gene:TRS31(YDR472W)|FD-Score:-3.91|P-value:4.65E-5|Clearance:0||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:YKT6(YKL196C)|FD-Score:3.93|P-value:4.31E-5|Clearance:0.41||SGD DESC:Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus Gene:YMR290W-A(YMR290W-A_d)|FD-Score:-3.51|P-value:2.23E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:BET1(YIL004C)|FD-Score:3.97|P-value:3.57E-5|Clearance:0.41||SGD DESC:Type II membrane protein required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins Gene:CCT7(YJL111W)|FD-Score:4.66|P-value:1.55E-6|Clearance:0.41||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CSE1(YGL238W)|FD-Score:3.16|P-value:7.76E-4|Clearance:0||SGD DESC:Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation Gene:ERG29(YMR134W_p)|FD-Score:-5.17|P-value:1.16E-7|Clearance:0||SGD DESC:Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes Gene:RPP1(YHR062C)|FD-Score:4.35|P-value:6.85E-6|Clearance:0.41||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRP9(YPR137W)|FD-Score:3.16|P-value:7.86E-4|Clearance:0.14||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:SEC27(YGL137W)|FD-Score:3.68|P-value:1.15E-4|Clearance:0.41||SGD DESC:Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP Gene:SPP2(YOR148C)|FD-Score:3.27|P-value:5.39E-4|Clearance:0.1||SGD DESC:Essential protein that promotes the first step of splicing and is required for the final stages of spliceosome maturation; interacts with Prp2p, which may release Spp2p from the spliceosome following the first cleavage reaction Gene:SRP14(YDL092W)|FD-Score:-4.15|P-value:1.66E-5|Clearance:0||SGD DESC:Signal recognition particle (SRP) subunit, interacts with the RNA component of SRP to form the Alu domain, which is the region of SRP responsible for arrest of nascent chain elongation during membrane targeting; homolog of mammalian SRP14 Gene:TIF11(YMR260C)|FD-Score:4.79|P-value:8.41E-7|Clearance:0.41||SGD DESC:Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3 Gene:TRS31(YDR472W)|FD-Score:-3.91|P-value:4.65E-5|Clearance:0||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:YKT6(YKL196C)|FD-Score:3.93|P-value:4.31E-5|Clearance:0.41||SGD DESC:Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus Gene:YMR290W-A(YMR290W-A_d)|FD-Score:-3.51|P-value:2.23E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3396
Download HOP data (tab-delimited text)  (excel)
Gene:AFT1(YGL071W)|FD-Score:3.41|P-value:3.26E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AIM18(YHR198C)|FD-Score:-3.13|P-value:8.83E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ARP5(YNL059C)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:CNM67(YNL225C)|FD-Score:3.83|P-value:6.33E-5||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:CRD1(YDL142C)|FD-Score:3.1|P-value:9.75E-4||SGD DESC:Cardiolipin synthase; produces cardiolipin, which is a phospholipid of the mitochondrial inner membrane that is required for normal mitochondrial membrane potential and function; also required for normal vacuolar ion homeostasis Gene:DAL80(YKR034W)|FD-Score:3.99|P-value:3.33E-5||SGD DESC:Negative regulator of genes in multiple nitrogen degradation pathways; expression is regulated by nitrogen levels and by Gln3p; member of the GATA-binding family, forms homodimers and heterodimers with Gzf3p; DAL80 has a paralog, GZF3, that arose from the whole genome duplication Gene:DSF2(YBR007C_p)|FD-Score:4.04|P-value:2.62E-5||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:EAP1(YKL204W)|FD-Score:4.23|P-value:1.15E-5||SGD DESC:eIF4E-associated protein, competes with eIF4G for binding to eIF4E; accelerates mRNA degradation by promoting decapping, facilitated by interaction with eIF4E; essential for Puf5p mediated repression; associates with Puf5p and Dhh1p; inhibits cap-dependent translation; functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade Gene:ECM11(YDR446W)|FD-Score:-3.71|P-value:1.04E-4||SGD DESC:Non-essential protein apparently involved in meiosis, GFP fusion protein is present in discrete clusters in the nucleus throughout mitosis; may be involved in maintaining chromatin structure Gene:ERG2(YMR202W)|FD-Score:14.9|P-value:2.93E-50||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG4(YGL012W)|FD-Score:4.16|P-value:1.60E-5||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:ERG5(YMR015C)|FD-Score:5.27|P-value:6.78E-8||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:ERV14(YGL054C)|FD-Score:7.26|P-value:1.93E-13||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FPR4(YLR449W)|FD-Score:3.25|P-value:5.73E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase) (proline isomerase) localized to the nucleus; catalyzes isomerization of proline residues in histones H3 and H4, which affects lysine methylation of those histones Gene:GCN5(YGR252W)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GEA1(YJR031C)|FD-Score:-3.43|P-value:3.01E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication Gene:GUP1(YGL084C)|FD-Score:-3.16|P-value:7.91E-4||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HOS1(YPR068C)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Class I histone deacetylase (HDAC) family member that deacetylates Smc3p on lysine residues at anaphase onset; has sequence similarity to Hda1p, Rpd3p, Hos2p, and Hos3p; interacts with the Tup1p-Ssn6p corepressor complex Gene:IMP2'(YIL154C)|FD-Score:6.04|P-value:7.80E-10||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:LEM3(YNL323W)|FD-Score:3.28|P-value:5.12E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:MEH1(YKR007W)|FD-Score:4.18|P-value:1.49E-5||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; loss results in a defect in vacuolar acidification Gene:MET32(YDR253C)|FD-Score:3.5|P-value:2.32E-4||SGD DESC:Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET32 has a paralog, MET31, that arose from the whole genome duplication Gene:MMM1(YLL006W)|FD-Score:-3.95|P-value:3.99E-5||SGD DESC:ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MSA1(YOR066W)|FD-Score:3.24|P-value:5.92E-4||SGD DESC:Activator of G1-specific transcription factors, MBF and SBF; involved in regulation of the timing of G1-specific gene transcription and cell cycle initiation; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:MSD1(YPL104W)|FD-Score:4.18|P-value:1.43E-5||SGD DESC:Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene Gene:NUP120(YKL057C)|FD-Score:4.47|P-value:3.94E-6||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160 Gene:OXA1(YER154W)|FD-Score:-4.2|P-value:1.36E-5||SGD DESC:Mitochondrial inner membrane insertase, mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane, interacts with mitochondrial ribosomes; conserved from bacteria to animals Gene:PEX28(YHR150W)|FD-Score:-3.26|P-value:5.62E-4||SGD DESC:Peroxisomal integral membrane peroxin, involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p Gene:PRR2(YDL214C)|FD-Score:3.15|P-value:8.06E-4||SGD DESC:Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor; mutant has increased aneuploidy tolerance Gene:PUS1(YPL212C)|FD-Score:4.68|P-value:1.43E-6||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; nuclear protein that appears to be involved in tRNA export; also acts on U2 snRNA Gene:PXL1(YKR090W)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Protein that localizes to sites of polarized growth; required for selection and/or maintenance of polarized growth sites, may modulate signaling by the GTPases Cdc42p and Rho1p; contains LIM domains and has similarity to metazoan paxillin; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RLF2(YPR018W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:SEA4(YBL104C)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites Gene:SFP1(YLR403W)|FD-Score:3.21|P-value:6.67E-4||SGD DESC:Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion Gene:SGE1(YPR198W)|FD-Score:-3.58|P-value:1.71E-4||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SSO2(YMR183C)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane; syntaxin homolog that is functionally redundant with Sso1p Gene:SSP120(YLR250W)|FD-Score:3.22|P-value:6.37E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:STV1(YMR054W)|FD-Score:3.79|P-value:7.67E-5||SGD DESC:Subunit a of the vacuolar-ATPase V0 domain, one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole Gene:VMA9(YCL005W-A)|FD-Score:3.95|P-value:3.86E-5||SGD DESC:Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis Gene:VPS45(YGL095C)|FD-Score:4.8|P-value:8.13E-7||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YML122C(YML122C_d)|FD-Score:3.25|P-value:5.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPK1(YKL126W)|FD-Score:9.32|P-value:5.79E-21||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL088W(YPL088W_p)|FD-Score:3.19|P-value:7.14E-4||SGD DESC:Putative aryl alcohol dehydrogenase; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance Gene:ZUO1(YGR285C)|FD-Score:3.83|P-value:6.43E-5||SGD DESC:Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p Gene:AFT1(YGL071W)|FD-Score:3.41|P-value:3.26E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AIM18(YHR198C)|FD-Score:-3.13|P-value:8.83E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ARP5(YNL059C)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:CNM67(YNL225C)|FD-Score:3.83|P-value:6.33E-5||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:CRD1(YDL142C)|FD-Score:3.1|P-value:9.75E-4||SGD DESC:Cardiolipin synthase; produces cardiolipin, which is a phospholipid of the mitochondrial inner membrane that is required for normal mitochondrial membrane potential and function; also required for normal vacuolar ion homeostasis Gene:DAL80(YKR034W)|FD-Score:3.99|P-value:3.33E-5||SGD DESC:Negative regulator of genes in multiple nitrogen degradation pathways; expression is regulated by nitrogen levels and by Gln3p; member of the GATA-binding family, forms homodimers and heterodimers with Gzf3p; DAL80 has a paralog, GZF3, that arose from the whole genome duplication Gene:DSF2(YBR007C_p)|FD-Score:4.04|P-value:2.62E-5||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:EAP1(YKL204W)|FD-Score:4.23|P-value:1.15E-5||SGD DESC:eIF4E-associated protein, competes with eIF4G for binding to eIF4E; accelerates mRNA degradation by promoting decapping, facilitated by interaction with eIF4E; essential for Puf5p mediated repression; associates with Puf5p and Dhh1p; inhibits cap-dependent translation; functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade Gene:ECM11(YDR446W)|FD-Score:-3.71|P-value:1.04E-4||SGD DESC:Non-essential protein apparently involved in meiosis, GFP fusion protein is present in discrete clusters in the nucleus throughout mitosis; may be involved in maintaining chromatin structure Gene:ERG2(YMR202W)|FD-Score:14.9|P-value:2.93E-50||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG4(YGL012W)|FD-Score:4.16|P-value:1.60E-5||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:ERG5(YMR015C)|FD-Score:5.27|P-value:6.78E-8||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:ERV14(YGL054C)|FD-Score:7.26|P-value:1.93E-13||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FPR4(YLR449W)|FD-Score:3.25|P-value:5.73E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase) (proline isomerase) localized to the nucleus; catalyzes isomerization of proline residues in histones H3 and H4, which affects lysine methylation of those histones Gene:GCN5(YGR252W)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GEA1(YJR031C)|FD-Score:-3.43|P-value:3.01E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication Gene:GUP1(YGL084C)|FD-Score:-3.16|P-value:7.91E-4||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HOS1(YPR068C)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Class I histone deacetylase (HDAC) family member that deacetylates Smc3p on lysine residues at anaphase onset; has sequence similarity to Hda1p, Rpd3p, Hos2p, and Hos3p; interacts with the Tup1p-Ssn6p corepressor complex Gene:IMP2'(YIL154C)|FD-Score:6.04|P-value:7.80E-10||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:LEM3(YNL323W)|FD-Score:3.28|P-value:5.12E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:MEH1(YKR007W)|FD-Score:4.18|P-value:1.49E-5||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; loss results in a defect in vacuolar acidification Gene:MET32(YDR253C)|FD-Score:3.5|P-value:2.32E-4||SGD DESC:Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET32 has a paralog, MET31, that arose from the whole genome duplication Gene:MMM1(YLL006W)|FD-Score:-3.95|P-value:3.99E-5||SGD DESC:ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MSA1(YOR066W)|FD-Score:3.24|P-value:5.92E-4||SGD DESC:Activator of G1-specific transcription factors, MBF and SBF; involved in regulation of the timing of G1-specific gene transcription and cell cycle initiation; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:MSD1(YPL104W)|FD-Score:4.18|P-value:1.43E-5||SGD DESC:Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene Gene:NUP120(YKL057C)|FD-Score:4.47|P-value:3.94E-6||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160 Gene:OXA1(YER154W)|FD-Score:-4.2|P-value:1.36E-5||SGD DESC:Mitochondrial inner membrane insertase, mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane, interacts with mitochondrial ribosomes; conserved from bacteria to animals Gene:PEX28(YHR150W)|FD-Score:-3.26|P-value:5.62E-4||SGD DESC:Peroxisomal integral membrane peroxin, involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p Gene:PRR2(YDL214C)|FD-Score:3.15|P-value:8.06E-4||SGD DESC:Serine/threonine protein kinase that inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor; mutant has increased aneuploidy tolerance Gene:PUS1(YPL212C)|FD-Score:4.68|P-value:1.43E-6||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; nuclear protein that appears to be involved in tRNA export; also acts on U2 snRNA Gene:PXL1(YKR090W)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Protein that localizes to sites of polarized growth; required for selection and/or maintenance of polarized growth sites, may modulate signaling by the GTPases Cdc42p and Rho1p; contains LIM domains and has similarity to metazoan paxillin; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RLF2(YPR018W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:SEA4(YBL104C)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites Gene:SFP1(YLR403W)|FD-Score:3.21|P-value:6.67E-4||SGD DESC:Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion Gene:SGE1(YPR198W)|FD-Score:-3.58|P-value:1.71E-4||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SSO2(YMR183C)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane; syntaxin homolog that is functionally redundant with Sso1p Gene:SSP120(YLR250W)|FD-Score:3.22|P-value:6.37E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:STV1(YMR054W)|FD-Score:3.79|P-value:7.67E-5||SGD DESC:Subunit a of the vacuolar-ATPase V0 domain, one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole Gene:VMA9(YCL005W-A)|FD-Score:3.95|P-value:3.86E-5||SGD DESC:Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis Gene:VPS45(YGL095C)|FD-Score:4.8|P-value:8.13E-7||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YML122C(YML122C_d)|FD-Score:3.25|P-value:5.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPK1(YKL126W)|FD-Score:9.32|P-value:5.79E-21||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL088W(YPL088W_p)|FD-Score:3.19|P-value:7.14E-4||SGD DESC:Putative aryl alcohol dehydrogenase; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance Gene:ZUO1(YGR285C)|FD-Score:3.83|P-value:6.43E-5||SGD DESC:Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YMR260C4.798.41E-70.41TIF11Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3
YJL111W4.661.55E-60.41CCT7Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance
YHR062C4.356.85E-60.41RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YIL004C3.973.57E-50.41BET1Type II membrane protein required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins
YKL196C3.934.31E-50.41YKT6Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus
YGL137W3.681.15E-40.41SEC27Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP
YOR148C3.275.39E-40.10SPP2Essential protein that promotes the first step of splicing and is required for the final stages of spliceosome maturation; interacts with Prp2p, which may release Spp2p from the spliceosome following the first cleavage reaction
YGL238W3.167.76E-40.00CSE1Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation
YPR137W3.167.86E-40.14RRP9Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein
YLR277C3.020.001270.10YSH1Putative endoribonuclease; subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of mRNAs and snoRNAs; protein abundance increases in response to DNA replication stress; YSH1 has a paralog, SYC1, that arose from the whole genome duplication
YOR260W2.920.001760.09GCD1Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YMR043W2.830.002350.03MCM1Transcription factor involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes
YOR232W2.800.002590.02MGE1Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE
YKL045W2.780.002750.07PRI2Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair
YLR197W2.700.003420.02NOP56Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR202W14.902.93E-50ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
YKL126W9.325.79E-21YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YGL054C7.261.93E-13ERV14COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication
YIL154C6.047.80E-10IMP2'Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat
YMR015C5.276.78E-8ERG5C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs
YGL095C4.808.13E-7VPS45Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment
YPL212C4.681.43E-6PUS1tRNA:pseudouridine synthase, introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; nuclear protein that appears to be involved in tRNA export; also acts on U2 snRNA
YKL057C4.473.94E-6NUP120Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160
YKL204W4.231.15E-5EAP1eIF4E-associated protein, competes with eIF4G for binding to eIF4E; accelerates mRNA degradation by promoting decapping, facilitated by interaction with eIF4E; essential for Puf5p mediated repression; associates with Puf5p and Dhh1p; inhibits cap-dependent translation; functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade
YPL104W4.181.43E-5MSD1Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene
YKR007W4.181.49E-5MEH1Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; loss results in a defect in vacuolar acidification
YGL012W4.161.60E-5ERG4C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol
YBR007C_p4.042.62E-5DSF2_pDeletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress
YKR034W3.993.33E-5DAL80Negative regulator of genes in multiple nitrogen degradation pathways; expression is regulated by nitrogen levels and by Gln3p; member of the GATA-binding family, forms homodimers and heterodimers with Gzf3p; DAL80 has a paralog, GZF3, that arose from the whole genome duplication
YCL005W-A3.953.86E-5VMA9Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis

GO enrichment analysis for SGTC_2720
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.4001.10E-225SGTC_234nsc-17383 10.7 μMMiscellaneous4089740.0681818ERG2
0.3836.25E-205SGTC_1826st055655 41.6 μMTimTec (Natural product derivative library)6761680.0941176ERG2
0.3531.11E-172SGTC_14504112-4260 37.6 μMChemDiv (Drug-like library)16331750.091954calcium & mitochondrial duress
0.3514.67E-170SGTC_21445628481 200.0 μMChembridge (Fragment library)28183990.0657895ERG2
0.3425.35E-161SGTC_7243910-0327 137.0 μMChemDiv (Drug-like library)28854830.0833333ERG2
0.3113.10E-132SGTC_12440416-0015 52.5 μMChemDiv (Drug-like library)962010.0967742ERG2
0.3031.96E-125SGTC_2732terfenadine 20.0 μMMiscellaneous54050.27027ERG2
0.3014.94E-124SGTC_2724maprotiline 69.6 μMMiscellaneous40110.136986ERG2
0.2973.12E-120SGTC_2734clemastine 41.6 μMMiscellaneous269870.123457ERG2
0.2932.01E-116SGTC_1464k208-0027 110.0 μMChemDiv (Drug-like library)X14640.153846
0.2878.25E-112SGTC_2523spermine 47.7 μMMicrosource (Natural product library)11030.0333333calcium & mitochondrial duress
0.2846.86E-110SGTC_8410251-0215 91.2 μMChemDiv (Drug-like library)38886140.192982
0.2775.42E-104SGTC_400oxethazaine 37.5 μMMiscellaneous46210.103896calcium & mitochondrial duress
0.2751.00E-102SGTC_540159-0055 9.0 μMChemDiv (Drug-like library)33190990.0843373ERG2
0.2751.22E-102SGTC_1627st003713 25.6 μMTimTec (Natural product derivative library)161950700.0891089calcium & mitochondrial duress

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_440fluspirilene35.1 μM13396ICCB bioactive library475.5727465.54515
SGTC_433pimozide108 μM0.46268716362Miscellaneous461.5461665.52114
SGTC_2249haloperidol116.36 μM0.2916673559Miscellaneous375.8642233.88714ergosterol depletion effects on membrane
SGTC_258haloperidol50.78 μM0.2916673559Miscellaneous375.8642233.88714fatty acid desaturase (OLE1)
SGTC_2725haloperidol53.21 μM0.2916673559Miscellaneous375.8642233.88714plasma membrane duress
SGTC_2732terfenadine20 μM0.270275405Miscellaneous471.673446.49823ERG2
SGTC_12830922-012516.4 μM0.253360884ChemDiv (Drug-like library)425.951024.84904
SGTC_10194262-02123.4 μM0.2285714677213ChemDiv (Drug-like library)352.4881635.20803ergosterol depletion effects on membrane
SGTC_3199911131849.47 μM0.2207798548020Chembridge (Drug-like library)381.3672733.73317
SGTC_2755lomerizine42.69 μM0.2162163949Miscellaneous468.5355065.41807
SGTC_14274048-4900550 nM0.21538524132586ChemDiv (Drug-like library)307.472365.79401
SGTC_2155557042376 μM0.215385797402Chembridge (Fragment library)250.3118632.54403ergosterol biosynthesis