maprotiline FDA approved compound

A tricyclic antidepressant.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2724
Screen concentration 69.6 μM
Source Miscellaneous
PubChem CID 4011
SMILES CNCCCC12CCC(C3=CC=CC=C31)C4=CC=CC=C24
Standardized SMILES CNCCCC12CCC(c3ccccc13)c4ccccc24
Molecular weight 277.4033
ALogP 4.12
H-bond donor count 1
H-bond acceptor count 1
Response signature ERG2

Pool Growth Kinetics
% growth inhibition (Het. pool) 7.94
% growth inhibition (Hom. pool) 2.43


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4011
Download HIP data (tab-delimited text)  (excel)
Gene:ACT1(YFL039C)|FD-Score:-3.7|P-value:1.07E-4|Clearance:0||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:GWT1(YJL091C)|FD-Score:3.3|P-value:4.86E-4|Clearance:0.3||SGD DESC:Protein involved in the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) to form glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors Gene:LSG1(YGL099W)|FD-Score:5.8|P-value:3.31E-9|Clearance:1.27||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MMS21(YEL019C)|FD-Score:3.67|P-value:1.20E-4|Clearance:0.26||SGD DESC:SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination Gene:NMD3(YHR170W)|FD-Score:4.53|P-value:2.97E-6|Clearance:0.25||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:OST1(YJL002C)|FD-Score:3.97|P-value:3.53E-5|Clearance:0.09||SGD DESC:Alpha subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:PBN1(YCL052C)|FD-Score:-3.33|P-value:4.27E-4|Clearance:0||SGD DESC:Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X Gene:RPS20(YHL015W)|FD-Score:-3.2|P-value:6.80E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:SDH3(YKL141W)|FD-Score:4.27|P-value:9.58E-6|Clearance:0.09||SGD DESC:Subunit of both succinate dehydrogenase and of TIM22 translocase; functions as the cytochrome b subunit of succinate dehydrogenase, which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex Gene:SEC27(YGL137W)|FD-Score:4.11|P-value:1.98E-5|Clearance:0.14||SGD DESC:Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP Gene:SPN1(YPR133C)|FD-Score:-4.94|P-value:3.97E-7|Clearance:0||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:SSY5(YJL156C)|FD-Score:4.18|P-value:1.43E-5|Clearance:0.07||SGD DESC:Serine protease of SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); contains an inhibitory domain that dissociates in response to extracellular amino acids, freeing a catalytic domain to activate transcription factor Stp1p Gene:TIF11(YMR260C)|FD-Score:3.35|P-value:4.09E-4|Clearance:0.05||SGD DESC:Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3 Gene:VHT1(YGR065C)|FD-Score:-3.45|P-value:2.82E-4|Clearance:0||SGD DESC:High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin Gene:YGR115C(YGR115C_d)|FD-Score:3.89|P-value:5.07E-5|Clearance:0.21||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YKT6(YKL196C)|FD-Score:3.41|P-value:3.25E-4|Clearance:0.06||SGD DESC:Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus Gene:ACT1(YFL039C)|FD-Score:-3.7|P-value:1.07E-4|Clearance:0||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:GWT1(YJL091C)|FD-Score:3.3|P-value:4.86E-4|Clearance:0.3||SGD DESC:Protein involved in the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) to form glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors Gene:LSG1(YGL099W)|FD-Score:5.8|P-value:3.31E-9|Clearance:1.27||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MMS21(YEL019C)|FD-Score:3.67|P-value:1.20E-4|Clearance:0.26||SGD DESC:SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination Gene:NMD3(YHR170W)|FD-Score:4.53|P-value:2.97E-6|Clearance:0.25||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:OST1(YJL002C)|FD-Score:3.97|P-value:3.53E-5|Clearance:0.09||SGD DESC:Alpha subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:PBN1(YCL052C)|FD-Score:-3.33|P-value:4.27E-4|Clearance:0||SGD DESC:Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X Gene:RPS20(YHL015W)|FD-Score:-3.2|P-value:6.80E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:SDH3(YKL141W)|FD-Score:4.27|P-value:9.58E-6|Clearance:0.09||SGD DESC:Subunit of both succinate dehydrogenase and of TIM22 translocase; functions as the cytochrome b subunit of succinate dehydrogenase, which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex Gene:SEC27(YGL137W)|FD-Score:4.11|P-value:1.98E-5|Clearance:0.14||SGD DESC:Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP Gene:SPN1(YPR133C)|FD-Score:-4.94|P-value:3.97E-7|Clearance:0||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:SSY5(YJL156C)|FD-Score:4.18|P-value:1.43E-5|Clearance:0.07||SGD DESC:Serine protease of SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); contains an inhibitory domain that dissociates in response to extracellular amino acids, freeing a catalytic domain to activate transcription factor Stp1p Gene:TIF11(YMR260C)|FD-Score:3.35|P-value:4.09E-4|Clearance:0.05||SGD DESC:Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3 Gene:VHT1(YGR065C)|FD-Score:-3.45|P-value:2.82E-4|Clearance:0||SGD DESC:High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin Gene:YGR115C(YGR115C_d)|FD-Score:3.89|P-value:5.07E-5|Clearance:0.21||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YKT6(YKL196C)|FD-Score:3.41|P-value:3.25E-4|Clearance:0.06||SGD DESC:Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4011
Download HOP data (tab-delimited text)  (excel)
Gene:ADE8(YDR408C)|FD-Score:-3.17|P-value:7.67E-4||SGD DESC:Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:AFT1(YGL071W)|FD-Score:4.31|P-value:8.34E-6||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AIM46(YHR199C)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:APL1(YJR005W)|FD-Score:3.17|P-value:7.64E-4||SGD DESC:Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex Gene:ARL1(YBR164C)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ATP15(YPL271W)|FD-Score:-3.13|P-value:8.60E-4||SGD DESC:Epsilon subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:BOP2(YLR267W_p)|FD-Score:3.76|P-value:8.66E-5||SGD DESC:Protein of unknown function Gene:CHD1(YER164W)|FD-Score:-3.41|P-value:3.20E-4||SGD DESC:Chromatin remodeler that regulates various aspects of transcription; acts in in conjunction with Isw1b to regulate chromatin structure and maintain chromatin integrity during transcription elongation by RNAP II by preventing trans-histone exchange over coding regions; contains a chromo domain, a helicase domain and a DNA-binding domain; component of both the SAGA and SLIK complexes Gene:CNB1(YKL190W)|FD-Score:4.3|P-value:8.46E-6||SGD DESC:Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress Gene:DCN1(YLR128W)|FD-Score:3.97|P-value:3.52E-5||SGD DESC:Scaffold-type E3 ligase required for cullin neddylation and ubiquitin ligase activation; contains a ubiquitin-binding domain (UBA) for ubiquitin and Nedd8 (Rub1p) interaction and a PONY domain involved in cullin binding and neddylation Gene:DDR2(YOL052C-A)|FD-Score:3.43|P-value:3.01E-4||SGD DESC:Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication Gene:DIT1(YDR403W)|FD-Score:3.24|P-value:5.91E-4||SGD DESC:Sporulation-specific enzyme required for spore wall maturation, involved in the production of a soluble LL-dityrosine-containing precursor of the spore wall; transcripts accumulate at the time of prospore enclosure Gene:ECM27(YJR106W)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants are hypersensitive to Papulacandin B; null mutants have increased plasmid loss; displays a two-hybrid interaction with Pdr5p Gene:EMI1(YDR512C)|FD-Score:-3.26|P-value:5.55E-4||SGD DESC:Non-essential protein required for transcriptional induction of the early meiotic-specific transcription factor IME1, also required for sporulation; contains twin cysteine-x9-cysteine motifs Gene:ERG2(YMR202W)|FD-Score:16.9|P-value:5.03E-64||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERV14(YGL054C)|FD-Score:9.66|P-value:2.17E-22||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FAR8(YMR029C)|FD-Score:4.36|P-value:6.38E-6||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p Gene:FRA2(YGL220W)|FD-Score:3.27|P-value:5.37E-4||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel Gene:GPD1(YDL022W)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity Gene:HYR1(YIR037W)|FD-Score:-3.49|P-value:2.46E-4||SGD DESC:Thiol peroxidase that functions as a hydroperoxide receptor to sense intracellular hydroperoxide levels and transduce a redox signal to the Yap1p transcription factor Gene:IDP2(YLR174W)|FD-Score:3.1|P-value:9.63E-4||SGD DESC:Cytosolic NADP-specific isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate; levels are elevated during growth on non-fermentable carbon sources and reduced during growth on glucose; IDP2 has a paralog, IDP3, that arose from the whole genome duplication Gene:IKI3(YLR384C)|FD-Score:-3.91|P-value:4.54E-5||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD) Gene:ITC1(YGL133W)|FD-Score:3.29|P-value:5.05E-4||SGD DESC:Subunit of the ATP-dependent Isw2p-Itc1p chromatin remodeling complex, required for repression of a-specific genes, repression of early meiotic genes during mitotic growth, and repression of INO1; similar to mammalian Acf1p, the regulatory subunit of the mammalian ATP-utilizing chromatin assembly and modifying factor (ACF) complex Gene:LSB6(YJL100W)|FD-Score:4.18|P-value:1.48E-5||SGD DESC:Type II phosphatidylinositol 4-kinase that binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization Gene:MGA2(YIR033W)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Spt23p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:MMS1(YPR164W)|FD-Score:3.26|P-value:5.55E-4||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; regulates Ty1 transposition; involved with Rtt101p in nonfunctional rRNA decay Gene:MOH1(YBL049W)|FD-Score:5.95|P-value:1.32E-9||SGD DESC:Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase Gene:MSA1(YOR066W)|FD-Score:-3.16|P-value:7.92E-4||SGD DESC:Activator of G1-specific transcription factors, MBF and SBF; involved in regulation of the timing of G1-specific gene transcription and cell cycle initiation; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:MSN4(YKL062W)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN4 has a paralog, MSN2, that arose from the whole genome duplication Gene:NUR1(YDL089W)|FD-Score:-4.76|P-value:9.86E-7||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:ODC1(YPL134C)|FD-Score:-3.43|P-value:3.02E-4||SGD DESC:Mitochondrial inner membrane transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for lysine and glutamate biosynthesis and lysine catabolism; suppresses, in multicopy, an fmc1 null mutation Gene:OPI6(YDL096C_d)|FD-Score:3.98|P-value:3.39E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential Gene:OXA1(YER154W)|FD-Score:-3.13|P-value:8.72E-4||SGD DESC:Mitochondrial inner membrane insertase, mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane, interacts with mitochondrial ribosomes; conserved from bacteria to animals Gene:PEP4(YPL154C)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Vacuolar aspartyl protease (proteinase A), required for the posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; synthesized as a zymogen, self-activates Gene:PET10(YKR046C)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Protein of unknown function that co-purifies with lipid particles; expression pattern suggests a role in respiratory growth; computational analysis of large-scale protein-protein interaction data suggests a role in ATP/ADP exchange Gene:RAD52(YML032C)|FD-Score:-3.09|P-value:9.85E-4||SGD DESC:Protein that stimulates strand exchange by facilitating Rad51p binding to single-stranded DNA; anneals complementary single-stranded DNA; involved in the repair of double-strand breaks in DNA during vegetative growth and meiosis Gene:REC104(YHR157W)|FD-Score:3.17|P-value:7.66E-4||SGD DESC:Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination Gene:RPS17B(YDR447C)|FD-Score:-4.17|P-value:1.54E-5||SGD DESC:Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17B has a paralog, RPS17A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPS6A(YPL090C)|FD-Score:-3.33|P-value:4.41E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6A has a paralog, RPS6B, that arose from the whole genome duplication Gene:SOL4(YGR248W)|FD-Score:4.88|P-value:5.35E-7||SGD DESC:6-phosphogluconolactonase; protein abundance increases in response to DNA replication stress; SOL4 has a paralog, SOL3, that arose from the whole genome duplication Gene:STE24(YJR117W)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans Gene:SUS1(YBR111W-A)|FD-Score:3.98|P-value:3.52E-5||SGD DESC:Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP Gene:SYH1(YPL105C)|FD-Score:3.31|P-value:4.65E-4||SGD DESC:Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication Gene:TDA3(YHR009C_p)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Putative oxidoreductase involved in late endosome to Golgi transport; physical and genetical interactions with Btn2p; null mutant is viable, has extended S phase, and sensitive to expression of top1-T722A allele; similar to human FOXRED1 Gene:TDA4(YJR116W_p)|FD-Score:4.05|P-value:2.59E-5||SGD DESC:Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele Gene:TUB3(YML124C)|FD-Score:-3.13|P-value:8.63E-4||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:UGA2(YBR006W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:VPS45(YGL095C)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YBL008W-A(YBL008W-A_p)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBL062W(YBL062W_d)|FD-Score:-3.19|P-value:7.12E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBL071C-B(YBL071C-B_p)|FD-Score:-4.61|P-value:2.02E-6||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YDR010C(YDR010C_d)|FD-Score:3.31|P-value:4.62E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR133C(YDR133C_d)|FD-Score:3.83|P-value:6.50E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR134C Gene:YDR537C(YDR537C_d)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, almost completely overlaps verified ORF PAD1/YDR538W Gene:YGR127W(YGR127W_p)|FD-Score:4.14|P-value:1.73E-5||SGD DESC:Putative protein of unknown function; expression is regulated by Msn2p/Msn4p, indicating a possible role in stress response Gene:YGR259C(YGR259C_d)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF TNA1/YGR260W Gene:YIL055C(YIL055C_p)|FD-Score:4.34|P-value:7.28E-6||SGD DESC:Putative protein of unknown function Gene:YLL020C(YLL020C_d)|FD-Score:4.1|P-value:2.04E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KNS1 Gene:YLR255C(YLR255C_d)|FD-Score:3.13|P-value:8.78E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR182W-A(YMR182W-A_p)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Putative protein of unknown function Gene:YNL140C(YNL140C_p)|FD-Score:-3.23|P-value:6.21E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps THO2/YNL139C Gene:YPL185W(YPL185W_d)|FD-Score:3.19|P-value:7.03E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene UIP4/YPL186C Gene:YPT31(YER031C)|FD-Score:5.04|P-value:2.34E-7||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication Gene:YPT35(YHR105W)|FD-Score:-3.11|P-value:9.39E-4||SGD DESC:Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport Gene:YPT6(YLR262C)|FD-Score:-3.29|P-value:5.05E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:ADE8(YDR408C)|FD-Score:-3.17|P-value:7.67E-4||SGD DESC:Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:AFT1(YGL071W)|FD-Score:4.31|P-value:8.34E-6||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AIM46(YHR199C)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:APL1(YJR005W)|FD-Score:3.17|P-value:7.64E-4||SGD DESC:Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex Gene:ARL1(YBR164C)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ATP15(YPL271W)|FD-Score:-3.13|P-value:8.60E-4||SGD DESC:Epsilon subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:BOP2(YLR267W_p)|FD-Score:3.76|P-value:8.66E-5||SGD DESC:Protein of unknown function Gene:CHD1(YER164W)|FD-Score:-3.41|P-value:3.20E-4||SGD DESC:Chromatin remodeler that regulates various aspects of transcription; acts in in conjunction with Isw1b to regulate chromatin structure and maintain chromatin integrity during transcription elongation by RNAP II by preventing trans-histone exchange over coding regions; contains a chromo domain, a helicase domain and a DNA-binding domain; component of both the SAGA and SLIK complexes Gene:CNB1(YKL190W)|FD-Score:4.3|P-value:8.46E-6||SGD DESC:Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress Gene:DCN1(YLR128W)|FD-Score:3.97|P-value:3.52E-5||SGD DESC:Scaffold-type E3 ligase required for cullin neddylation and ubiquitin ligase activation; contains a ubiquitin-binding domain (UBA) for ubiquitin and Nedd8 (Rub1p) interaction and a PONY domain involved in cullin binding and neddylation Gene:DDR2(YOL052C-A)|FD-Score:3.43|P-value:3.01E-4||SGD DESC:Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication Gene:DIT1(YDR403W)|FD-Score:3.24|P-value:5.91E-4||SGD DESC:Sporulation-specific enzyme required for spore wall maturation, involved in the production of a soluble LL-dityrosine-containing precursor of the spore wall; transcripts accumulate at the time of prospore enclosure Gene:ECM27(YJR106W)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants are hypersensitive to Papulacandin B; null mutants have increased plasmid loss; displays a two-hybrid interaction with Pdr5p Gene:EMI1(YDR512C)|FD-Score:-3.26|P-value:5.55E-4||SGD DESC:Non-essential protein required for transcriptional induction of the early meiotic-specific transcription factor IME1, also required for sporulation; contains twin cysteine-x9-cysteine motifs Gene:ERG2(YMR202W)|FD-Score:16.9|P-value:5.03E-64||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERV14(YGL054C)|FD-Score:9.66|P-value:2.17E-22||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FAR8(YMR029C)|FD-Score:4.36|P-value:6.38E-6||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p Gene:FRA2(YGL220W)|FD-Score:3.27|P-value:5.37E-4||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel Gene:GPD1(YDL022W)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity Gene:HYR1(YIR037W)|FD-Score:-3.49|P-value:2.46E-4||SGD DESC:Thiol peroxidase that functions as a hydroperoxide receptor to sense intracellular hydroperoxide levels and transduce a redox signal to the Yap1p transcription factor Gene:IDP2(YLR174W)|FD-Score:3.1|P-value:9.63E-4||SGD DESC:Cytosolic NADP-specific isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate; levels are elevated during growth on non-fermentable carbon sources and reduced during growth on glucose; IDP2 has a paralog, IDP3, that arose from the whole genome duplication Gene:IKI3(YLR384C)|FD-Score:-3.91|P-value:4.54E-5||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD) Gene:ITC1(YGL133W)|FD-Score:3.29|P-value:5.05E-4||SGD DESC:Subunit of the ATP-dependent Isw2p-Itc1p chromatin remodeling complex, required for repression of a-specific genes, repression of early meiotic genes during mitotic growth, and repression of INO1; similar to mammalian Acf1p, the regulatory subunit of the mammalian ATP-utilizing chromatin assembly and modifying factor (ACF) complex Gene:LSB6(YJL100W)|FD-Score:4.18|P-value:1.48E-5||SGD DESC:Type II phosphatidylinositol 4-kinase that binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization Gene:MGA2(YIR033W)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Spt23p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:MMS1(YPR164W)|FD-Score:3.26|P-value:5.55E-4||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; regulates Ty1 transposition; involved with Rtt101p in nonfunctional rRNA decay Gene:MOH1(YBL049W)|FD-Score:5.95|P-value:1.32E-9||SGD DESC:Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase Gene:MSA1(YOR066W)|FD-Score:-3.16|P-value:7.92E-4||SGD DESC:Activator of G1-specific transcription factors, MBF and SBF; involved in regulation of the timing of G1-specific gene transcription and cell cycle initiation; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:MSN4(YKL062W)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN4 has a paralog, MSN2, that arose from the whole genome duplication Gene:NUR1(YDL089W)|FD-Score:-4.76|P-value:9.86E-7||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:ODC1(YPL134C)|FD-Score:-3.43|P-value:3.02E-4||SGD DESC:Mitochondrial inner membrane transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for lysine and glutamate biosynthesis and lysine catabolism; suppresses, in multicopy, an fmc1 null mutation Gene:OPI6(YDL096C_d)|FD-Score:3.98|P-value:3.39E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential Gene:OXA1(YER154W)|FD-Score:-3.13|P-value:8.72E-4||SGD DESC:Mitochondrial inner membrane insertase, mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane, interacts with mitochondrial ribosomes; conserved from bacteria to animals Gene:PEP4(YPL154C)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Vacuolar aspartyl protease (proteinase A), required for the posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; synthesized as a zymogen, self-activates Gene:PET10(YKR046C)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Protein of unknown function that co-purifies with lipid particles; expression pattern suggests a role in respiratory growth; computational analysis of large-scale protein-protein interaction data suggests a role in ATP/ADP exchange Gene:RAD52(YML032C)|FD-Score:-3.09|P-value:9.85E-4||SGD DESC:Protein that stimulates strand exchange by facilitating Rad51p binding to single-stranded DNA; anneals complementary single-stranded DNA; involved in the repair of double-strand breaks in DNA during vegetative growth and meiosis Gene:REC104(YHR157W)|FD-Score:3.17|P-value:7.66E-4||SGD DESC:Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination Gene:RPS17B(YDR447C)|FD-Score:-4.17|P-value:1.54E-5||SGD DESC:Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17B has a paralog, RPS17A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPS6A(YPL090C)|FD-Score:-3.33|P-value:4.41E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6A has a paralog, RPS6B, that arose from the whole genome duplication Gene:SOL4(YGR248W)|FD-Score:4.88|P-value:5.35E-7||SGD DESC:6-phosphogluconolactonase; protein abundance increases in response to DNA replication stress; SOL4 has a paralog, SOL3, that arose from the whole genome duplication Gene:STE24(YJR117W)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans Gene:SUS1(YBR111W-A)|FD-Score:3.98|P-value:3.52E-5||SGD DESC:Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP Gene:SYH1(YPL105C)|FD-Score:3.31|P-value:4.65E-4||SGD DESC:Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication Gene:TDA3(YHR009C_p)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Putative oxidoreductase involved in late endosome to Golgi transport; physical and genetical interactions with Btn2p; null mutant is viable, has extended S phase, and sensitive to expression of top1-T722A allele; similar to human FOXRED1 Gene:TDA4(YJR116W_p)|FD-Score:4.05|P-value:2.59E-5||SGD DESC:Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele Gene:TUB3(YML124C)|FD-Score:-3.13|P-value:8.63E-4||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:UGA2(YBR006W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:VPS45(YGL095C)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YBL008W-A(YBL008W-A_p)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBL062W(YBL062W_d)|FD-Score:-3.19|P-value:7.12E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBL071C-B(YBL071C-B_p)|FD-Score:-4.61|P-value:2.02E-6||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YDR010C(YDR010C_d)|FD-Score:3.31|P-value:4.62E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR133C(YDR133C_d)|FD-Score:3.83|P-value:6.50E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR134C Gene:YDR537C(YDR537C_d)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, almost completely overlaps verified ORF PAD1/YDR538W Gene:YGR127W(YGR127W_p)|FD-Score:4.14|P-value:1.73E-5||SGD DESC:Putative protein of unknown function; expression is regulated by Msn2p/Msn4p, indicating a possible role in stress response Gene:YGR259C(YGR259C_d)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF TNA1/YGR260W Gene:YIL055C(YIL055C_p)|FD-Score:4.34|P-value:7.28E-6||SGD DESC:Putative protein of unknown function Gene:YLL020C(YLL020C_d)|FD-Score:4.1|P-value:2.04E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KNS1 Gene:YLR255C(YLR255C_d)|FD-Score:3.13|P-value:8.78E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR182W-A(YMR182W-A_p)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Putative protein of unknown function Gene:YNL140C(YNL140C_p)|FD-Score:-3.23|P-value:6.21E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps THO2/YNL139C Gene:YPL185W(YPL185W_d)|FD-Score:3.19|P-value:7.03E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene UIP4/YPL186C Gene:YPT31(YER031C)|FD-Score:5.04|P-value:2.34E-7||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication Gene:YPT35(YHR105W)|FD-Score:-3.11|P-value:9.39E-4||SGD DESC:Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport Gene:YPT6(YLR262C)|FD-Score:-3.29|P-value:5.05E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL099W5.803.31E-91.27LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YHR170W4.532.97E-60.25NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YKL141W4.279.58E-60.09SDH3Subunit of both succinate dehydrogenase and of TIM22 translocase; functions as the cytochrome b subunit of succinate dehydrogenase, which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex
YJL156C4.181.43E-50.07SSY5Serine protease of SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); contains an inhibitory domain that dissociates in response to extracellular amino acids, freeing a catalytic domain to activate transcription factor Stp1p
YGL137W4.111.98E-50.14SEC27Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP
YJL002C3.973.53E-50.09OST1Alpha subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins
YGR115C_d3.895.07E-50.21YGR115C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C
YEL019C3.671.20E-40.26MMS21SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination
YKL196C3.413.25E-40.06YKT6Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus
YMR260C3.354.09E-40.05TIF11Translation initiation factor eIF1A, essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3
YJL091C3.304.86E-40.30GWT1Protein involved in the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) to form glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors
YDR412W2.990.001380.03RRP17Component of the pre-60S pre-ribosomal particle; required for cell viability under standard (aerobic) conditions but not under anaerobic conditions; exonuclease required for 5′ end processing of pre-60S ribosomal RNA
YBL020W2.960.001530.05RFT1Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein
YKL203C2.920.001770.05TOR2PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
YOR218C_d2.870.002080.04YOR218C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene RFC1/YOR217W

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR202W16.905.03E-64ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
YGL054C9.662.17E-22ERV14COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication
YBL049W5.951.32E-9MOH1Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase
YER031C5.042.34E-7YPT31Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication
YGR248W4.885.35E-7SOL46-phosphogluconolactonase; protein abundance increases in response to DNA replication stress; SOL4 has a paralog, SOL3, that arose from the whole genome duplication
YMR029C4.366.38E-6FAR8Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p
YIL055C_p4.347.28E-6YIL055C_pPutative protein of unknown function
YGL071W4.318.34E-6AFT1Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
YKL190W4.308.46E-6CNB1Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress
YHR009C_p4.261.03E-5TDA3_pPutative oxidoreductase involved in late endosome to Golgi transport; physical and genetical interactions with Btn2p; null mutant is viable, has extended S phase, and sensitive to expression of top1-T722A allele; similar to human FOXRED1
YJL100W4.181.48E-5LSB6Type II phosphatidylinositol 4-kinase that binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization
YGR127W_p4.141.73E-5YGR127W_pPutative protein of unknown function; expression is regulated by Msn2p/Msn4p, indicating a possible role in stress response
YLL020C_d4.102.04E-5YLL020C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KNS1
YJR116W_p4.052.59E-5TDA4_pPutative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele
YDL096C_d3.983.39E-5OPI6_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential

GO enrichment analysis for SGTC_2724
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.3253.29E-145SGTC_2734clemastine 41.6 μMMiscellaneous269870.151515ERG2
0.3014.94E-124SGTC_2720fluspirilene 36.9 μMMiscellaneous33960.136986ERG2
0.3007.53E-123SGTC_12440416-0015 52.5 μMChemDiv (Drug-like library)962010.12766ERG2
0.2925.43E-116SGTC_234nsc-17383 10.7 μMMiscellaneous4089740.0972222ERG2
0.2723.91E-100SGTC_21445628481 200.0 μMChembridge (Fragment library)28183990.118644ERG2
0.2681.25E-97SGTC_28207989630 71.4 μMChembridge (Drug-like library)29840980.0958904ERG2
0.2608.90E-92SGTC_12063978-0017 132.0 μMChemDiv (Drug-like library)38736770.0897436ERG2
0.2593.64E-91SGTC_1826st055655 41.6 μMTimTec (Natural product derivative library)6761680.0985916ERG2
0.2483.51E-83SGTC_12470448-0043 5.8 μMChemDiv (Drug-like library)30890130.078125ERG2
0.2282.50E-70SGTC_20555235184 200.0 μMChembridge (Fragment library)28360340.0714286ERG2
0.2251.18E-68SGTC_13601598-0022 39.8 μMChemDiv (Drug-like library)7615250.118644ERG2
0.2251.51E-68SGTC_2699st077704 54.6 μMTimTec (Natural product derivative library)6458520.1endomembrane recycling
0.2109.87E-60SGTC_2694st077685 81.9 μMTimTec (Natural product derivative library)28380960.151515endomembrane recycling
0.2011.00E-54SGTC_9541189-0853 2.9 μMChemDiv (Drug-like library)28281940.137931endomembrane recycling
0.1982.40E-53SGTC_7243910-0327 137.0 μMChemDiv (Drug-like library)28854830.101449ERG2

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1081sertraline6 μM0.25925963009NIH Clinical Collection342.690525.3782160S ribosome export
SGTC_1070indatraline8.81 μM0.24074110314472NIH Clinical Collection328.663944.9222160S ribosome export
SGTC_25303-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene92.62 μM0.2407416708760Microsource (Natural product library)216.31873.35611
SGTC_2771fluoxetine41.05 μM0.2068973386Miscellaneous309.326134.03315
SGTC_3240913352749.47 μM0.217617455Chembridge (Drug-like library)349.4197033.01215
SGTC_12300170-03653.77 μM0.1967214018932ChemDiv (Drug-like library)317.50876.36312endomembrane recycling
SGTC_1094duloxetine25.4 μM0.19354860834NIH Clinical Collection333.87554.22823RPP1 & pyrimidine depletion
SGTC_1103duloxetine36.1 μM0.19354860835NIH Clinical Collection297.414563.84613
SGTC_1752st04481983.04 μM0.188679441209TimTec (Natural product derivative library)204.178821.45304
SGTC_11463579-10237 μM0.1875X1146ChemDiv (Drug-like library)381.507835.48843
SGTC_7420kpi-0133140 μM0.1864412728852ChemDiv (Drug-like library)297.434483.98912ERG2