cimetidine FDA approved compound

1-cyano-2-methyl-3-[2-[(5-methyl-1H-imidazol-4-yl)methylsulfanyl]ethyl]guanidine

A P450 inhibitor, histamine H2-receptor antagonist.

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 

PubChem MeSH terms: Anti-Ulcer Agents;Enzyme Inhibitors;Histamine H2 Antagonists



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2726
Screen concentration 79.3 μM
Source Miscellaneous
PubChem CID 2756
SMILES CC1=C(N=CN1)CSCCNC(=NC)NC#N
Standardized SMILES CN=C(NCCSCc1[nH]cnc1C)NC#N
Molecular weight 252.3392
ALogP 0.01
H-bond donor count 3
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.74
% growth inhibition (Hom. pool) 1.31


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2756
Download HIP data (tab-delimited text)  (excel)
Gene:MTR4(YJL050W)|FD-Score:-3.54|P-value:2.03E-4|Clearance:0||SGD DESC:ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing Gene:NSE3(YDR288W)|FD-Score:3.69|P-value:1.12E-4|Clearance:0.63||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress Gene:NUP159(YIL115C)|FD-Score:4.41|P-value:5.12E-6|Clearance:0.63||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p) Gene:NUP49(YGL172W)|FD-Score:4.22|P-value:1.25E-5|Clearance:0.63||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:QRI1(YDL103C)|FD-Score:-3.98|P-value:3.49E-5|Clearance:0||SGD DESC:UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress Gene:RER2(YBR002C)|FD-Score:4.63|P-value:1.81E-6|Clearance:0.63||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:SPP2(YOR148C)|FD-Score:-3.54|P-value:1.99E-4|Clearance:0||SGD DESC:Essential protein that promotes the first step of splicing and is required for the final stages of spliceosome maturation; interacts with Prp2p, which may release Spp2p from the spliceosome following the first cleavage reaction Gene:TIM22(YDL217C)|FD-Score:-3.63|P-value:1.41E-4|Clearance:0||SGD DESC:Essential core component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported Gene:MTR4(YJL050W)|FD-Score:-3.54|P-value:2.03E-4|Clearance:0||SGD DESC:ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing Gene:NSE3(YDR288W)|FD-Score:3.69|P-value:1.12E-4|Clearance:0.63||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress Gene:NUP159(YIL115C)|FD-Score:4.41|P-value:5.12E-6|Clearance:0.63||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p) Gene:NUP49(YGL172W)|FD-Score:4.22|P-value:1.25E-5|Clearance:0.63||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:QRI1(YDL103C)|FD-Score:-3.98|P-value:3.49E-5|Clearance:0||SGD DESC:UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress Gene:RER2(YBR002C)|FD-Score:4.63|P-value:1.81E-6|Clearance:0.63||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:SPP2(YOR148C)|FD-Score:-3.54|P-value:1.99E-4|Clearance:0||SGD DESC:Essential protein that promotes the first step of splicing and is required for the final stages of spliceosome maturation; interacts with Prp2p, which may release Spp2p from the spliceosome following the first cleavage reaction Gene:TIM22(YDL217C)|FD-Score:-3.63|P-value:1.41E-4|Clearance:0||SGD DESC:Essential core component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2756
Download HOP data (tab-delimited text)  (excel)
Gene:ADE12(YNL220W)|FD-Score:-4.4|P-value:5.49E-6||SGD DESC:Adenylosuccinate synthase, catalyzes the first step in synthesis of adenosine monophosphate from inosine 5'monophosphate during purine nucleotide biosynthesis; exhibits binding to single-stranded autonomously replicating (ARS) core sequence Gene:AIM18(YHR198C)|FD-Score:5.45|P-value:2.56E-8||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ALK1(YGL021W)|FD-Score:-3.22|P-value:6.46E-4||SGD DESC:Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; ALK1 has a paralog, ALK2, that arose from the whole genome duplication; similar to mammalian haspins Gene:APL1(YJR005W)|FD-Score:3.11|P-value:9.46E-4||SGD DESC:Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex Gene:BGL2(YGR282C)|FD-Score:4.46|P-value:4.13E-6||SGD DESC:Endo-beta-1,3-glucanase, major protein of the cell wall, involved in cell wall maintenance Gene:DBP7(YKR024C)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions Gene:HUR1(YGL168W)|FD-Score:4.18|P-value:1.45E-5||SGD DESC:Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene Gene:HYR1(YIR037W)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Thiol peroxidase that functions as a hydroperoxide receptor to sense intracellular hydroperoxide levels and transduce a redox signal to the Yap1p transcription factor Gene:LIP2(YLR239C)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:MLH3(YPL164C)|FD-Score:3.17|P-value:7.66E-4||SGD DESC:Protein involved in DNA mismatch repair and crossing-over during meiotic recombination; forms a complex with Mlh1p; mammalian homolog is implicated mammalian microsatellite instability Gene:MSB4(YOL112W)|FD-Score:4.62|P-value:1.94E-6||SGD DESC:GTPase-activating protein of the Ras superfamily that acts primarily on Sec4p, localizes to the bud site and bud tip, has similarity to Msb3p; msb3 msb4 double mutation causes defects in secretion and actin organization Gene:MXR1(YER042W)|FD-Score:3.28|P-value:5.24E-4||SGD DESC:Methionine-S-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR2; involved in the regulation of lifespan Gene:NAB6(YML117W)|FD-Score:-3.86|P-value:5.60E-5||SGD DESC:Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP Gene:OPI6(YDL096C_d)|FD-Score:-3.78|P-value:7.86E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential Gene:PCS60(YBR222C)|FD-Score:-3.21|P-value:6.55E-4||SGD DESC:Peroxisomal protein that binds AMP and mRNA, localizes to both the peroxisomal peripheral membrane and matrix, expression is highly inducible by oleic acid, similar to E. coli long chain acyl-CoA synthetase Gene:PGM1(YKL127W)|FD-Score:-4.51|P-value:3.20E-6||SGD DESC:Phosphoglucomutase, minor isoform; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism Gene:RHR2(YIL053W)|FD-Score:5.46|P-value:2.42E-8||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:RNR4(YGR180C)|FD-Score:3.26|P-value:5.64E-4||SGD DESC:Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:RRT1(YBL048W_d)|FD-Score:3.14|P-value:8.56E-4||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:SOL2(YCR073W-A)|FD-Score:5.56|P-value:1.32E-8||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication Gene:SUS1(YBR111W-A)|FD-Score:4.19|P-value:1.42E-5||SGD DESC:Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP Gene:UBP14(YBR058C)|FD-Score:3.37|P-value:3.71E-4||SGD DESC:Ubiquitin-specific protease that specifically disassembles unanchored ubiquitin chains; involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; similar to human isopeptidase T Gene:YCL001W-A(YCL001W-A_p)|FD-Score:3.76|P-value:8.37E-5||SGD DESC:Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III Gene:YFL041W-A(YFL041W-A_p)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YJL016W(YJL016W_p)|FD-Score:3.15|P-value:8.04E-4||SGD DESC:Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; conserved in closely related Saccharomyces species Gene:YLR030W(YLR030W_p)|FD-Score:-3.64|P-value:1.34E-4||SGD DESC:Putative protein of unknown function Gene:YOR139C(YOR139C_d)|FD-Score:3.4|P-value:3.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W Gene:YPS7(YDR349C)|FD-Score:-3.24|P-value:5.92E-4||SGD DESC:Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; located in the cytoplasm and endoplasmic reticulum Gene:ADE12(YNL220W)|FD-Score:-4.4|P-value:5.49E-6||SGD DESC:Adenylosuccinate synthase, catalyzes the first step in synthesis of adenosine monophosphate from inosine 5'monophosphate during purine nucleotide biosynthesis; exhibits binding to single-stranded autonomously replicating (ARS) core sequence Gene:AIM18(YHR198C)|FD-Score:5.45|P-value:2.56E-8||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ALK1(YGL021W)|FD-Score:-3.22|P-value:6.46E-4||SGD DESC:Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; ALK1 has a paralog, ALK2, that arose from the whole genome duplication; similar to mammalian haspins Gene:APL1(YJR005W)|FD-Score:3.11|P-value:9.46E-4||SGD DESC:Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex Gene:BGL2(YGR282C)|FD-Score:4.46|P-value:4.13E-6||SGD DESC:Endo-beta-1,3-glucanase, major protein of the cell wall, involved in cell wall maintenance Gene:DBP7(YKR024C)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions Gene:HUR1(YGL168W)|FD-Score:4.18|P-value:1.45E-5||SGD DESC:Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene Gene:HYR1(YIR037W)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Thiol peroxidase that functions as a hydroperoxide receptor to sense intracellular hydroperoxide levels and transduce a redox signal to the Yap1p transcription factor Gene:LIP2(YLR239C)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:MLH3(YPL164C)|FD-Score:3.17|P-value:7.66E-4||SGD DESC:Protein involved in DNA mismatch repair and crossing-over during meiotic recombination; forms a complex with Mlh1p; mammalian homolog is implicated mammalian microsatellite instability Gene:MSB4(YOL112W)|FD-Score:4.62|P-value:1.94E-6||SGD DESC:GTPase-activating protein of the Ras superfamily that acts primarily on Sec4p, localizes to the bud site and bud tip, has similarity to Msb3p; msb3 msb4 double mutation causes defects in secretion and actin organization Gene:MXR1(YER042W)|FD-Score:3.28|P-value:5.24E-4||SGD DESC:Methionine-S-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR2; involved in the regulation of lifespan Gene:NAB6(YML117W)|FD-Score:-3.86|P-value:5.60E-5||SGD DESC:Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP Gene:OPI6(YDL096C_d)|FD-Score:-3.78|P-value:7.86E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential Gene:PCS60(YBR222C)|FD-Score:-3.21|P-value:6.55E-4||SGD DESC:Peroxisomal protein that binds AMP and mRNA, localizes to both the peroxisomal peripheral membrane and matrix, expression is highly inducible by oleic acid, similar to E. coli long chain acyl-CoA synthetase Gene:PGM1(YKL127W)|FD-Score:-4.51|P-value:3.20E-6||SGD DESC:Phosphoglucomutase, minor isoform; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism Gene:RHR2(YIL053W)|FD-Score:5.46|P-value:2.42E-8||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:RNR4(YGR180C)|FD-Score:3.26|P-value:5.64E-4||SGD DESC:Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:RRT1(YBL048W_d)|FD-Score:3.14|P-value:8.56E-4||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:SOL2(YCR073W-A)|FD-Score:5.56|P-value:1.32E-8||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication Gene:SUS1(YBR111W-A)|FD-Score:4.19|P-value:1.42E-5||SGD DESC:Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP Gene:UBP14(YBR058C)|FD-Score:3.37|P-value:3.71E-4||SGD DESC:Ubiquitin-specific protease that specifically disassembles unanchored ubiquitin chains; involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; similar to human isopeptidase T Gene:YCL001W-A(YCL001W-A_p)|FD-Score:3.76|P-value:8.37E-5||SGD DESC:Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III Gene:YFL041W-A(YFL041W-A_p)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YJL016W(YJL016W_p)|FD-Score:3.15|P-value:8.04E-4||SGD DESC:Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; conserved in closely related Saccharomyces species Gene:YLR030W(YLR030W_p)|FD-Score:-3.64|P-value:1.34E-4||SGD DESC:Putative protein of unknown function Gene:YOR139C(YOR139C_d)|FD-Score:3.4|P-value:3.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W Gene:YPS7(YDR349C)|FD-Score:-3.24|P-value:5.92E-4||SGD DESC:Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; located in the cytoplasm and endoplasmic reticulum

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBR002C4.631.81E-60.63RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YIL115C4.415.12E-60.63NUP159FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p)
YGL172W4.221.25E-50.63NUP49FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p)
YDR288W3.691.12E-40.63NSE3Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress
YOL021C3.060.001100.01DIS3Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress
YDL150W3.050.001130.19RPC53RNA polymerase III subunit C53
YJL202C_d2.860.002090.03YJL202C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 3' end of essential PRP21 gene encoding a subunit of the SF3a splicing factor complex
YGL093W2.830.002290.03SPC105Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components
YJR064W2.810.002510.01CCT5Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YDL143W2.790.002620.04CCT4Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YPR137W2.750.002960.10RRP9Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein
YJL009W_d2.650.004050.04YJL009W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex
YMR168C2.610.004540.01CEP3Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
YML085C2.600.004710.02TUB1Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication
YJL010C2.570.005040.06NOP9Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YCR073W-A5.561.32E-8SOL2Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication
YIL053W5.462.42E-8RHR2Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication
YHR198C5.452.56E-8AIM18Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss
YOL112W4.621.94E-6MSB4GTPase-activating protein of the Ras superfamily that acts primarily on Sec4p, localizes to the bud site and bud tip, has similarity to Msb3p; msb3 msb4 double mutation causes defects in secretion and actin organization
YGR282C4.464.13E-6BGL2Endo-beta-1,3-glucanase, major protein of the cell wall, involved in cell wall maintenance
YBR111W-A4.191.42E-5SUS1Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP
YGL168W4.181.45E-5HUR1Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene
YCL001W-A_p3.768.37E-5YCL001W-A_pPutative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III
YFL041W-A_p3.551.90E-4YFL041W-A_pPutative protein of unknown function; identified by fungal homology and RT-PCR
YLR239C3.452.85E-4LIP2Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups
YOR139C_d3.403.37E-4YOR139C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W
YBR058C3.373.71E-4UBP14Ubiquitin-specific protease that specifically disassembles unanchored ubiquitin chains; involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; similar to human isopeptidase T
YER042W3.285.24E-4MXR1Methionine-S-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR2; involved in the regulation of lifespan
YIR037W3.265.52E-4HYR1Thiol peroxidase that functions as a hydroperoxide receptor to sense intracellular hydroperoxide levels and transduce a redox signal to the Yap1p transcription factor
YGR180C3.265.64E-4RNR4Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress

GO enrichment analysis for SGTC_2726
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0921.25E-12SGTC_32279130768 49.5 μMChembridge (Drug-like library)177208990.0697674
0.0881.23E-11SGTC_2745rifampicin 24.3 μMMiscellaneous251414280.0454545
0.0808.32E-10SGTC_1863455-3465 67.3 μMChemDiv (Drug-like library)7552780.106061
0.0782.17E-9SGTC_1390099-0334 718.6 μMChemDiv (Drug-like library)976900.0857143
0.0782.39E-9SGTC_305-(2-thienylidene)-Rhodanine 28.7 μMChemDiv (Drug-like library)12411320.0273973
0.0741.15E-8SGTC_30919116372 49.5 μMChembridge (Drug-like library)171697930.0933333
0.0731.64E-8SGTC_1116jasmonic acid  1.6 μMTimTec (Natural product library)52811660.0547945
0.0723.43E-8SGTC_2231micatex 6.9 μMMiscellaneous207370.0689655
0.0714.28E-8SGTC_2728chlorpromazine 9.2 μMMiscellaneous27260.0519481
0.0715.06E-8SGTC_1723st034307 2.6 μMTimTec (Natural product derivative library)8674660.028169mitochondrial processes
0.0672.66E-7SGTC_7661391-0721 126.0 μMChemDiv (Drug-like library)10209190.0795455
0.0672.86E-7SGTC_2727riluzole 51.8 μMMiscellaneous50700.0422535mitochondrial processes
0.0649.03E-7SGTC_190279-0214 38.4 μMChemDiv (Drug-like library)35662740.030303TRP & mitochondrial translation
0.0631.25E-6SGTC_1360097-0015 404.5 μMChemDiv (Drug-like library)48613370.028985560S ribosome export
0.0621.99E-6SGTC_2505galangin 7.9 μMMicrosource (Natural product library)52816160.027027RPP1 & pyrimidine depletion

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1819st05344858.6 μM0.1780822857616TimTec (Natural product derivative library)300.378743.44715amide catabolism
SGTC_1755st04196045.19 μM0.1666672257073TimTec (Natural product derivative library)328.43194.15515
SGTC_23916285546200 μM0.151515846083Chembridge (Fragment library)208.644262.1612
SGTC_1769st04440664.4 μM0.150685670903TimTec (Natural product derivative library)310.3487433.5415
SGTC_11881403-00144.57 μM0.1492549614867ChemDiv (Drug-like library)297.174263.14225mitochondrial stress
SGTC_1641st00929947.1 μM0.1388894046054TimTec (Natural product derivative library)290.771243.46814mitochondrial processes
SGTC_3174495-0002170 μM0.13698615991618ChemDiv (Drug-like library)351.26143.07613
SGTC_20185133068143 μM0.134328785362Chembridge (Fragment library)186.20992.0212
SGTC_3273913785949.47 μM0.1343282377234Chembridge (Drug-like library)266.360764.53803
SGTC_1106tegaserod maleate11.7 μM0.1341466918369NIH Clinical Collection417.458881.87758