hydroxyurea FDA approved compound

hydroxyurea


Established Yeast Drug Target : RNR1 RNR2 RNR3 RNR4

Anti-neoplastic. Inactivates ribonucleoside reductase by forming a free radical nitroxide that binds a tyrosyl free radical in the active site of the enzyme. This blocks the synthesis of deoxynucleotides, which inhibits DNA synthesis and induces synchronization or cell death in S-phase.

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 

PubChem MeSH terms: Antineoplastic Agents;Antisickling Agents;Enzyme Inhibitors;Nucleic Acid Synthesis Inhibitors



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_273
Screen concentration 18.1 mM
Source Miscellaneous
PubChem CID 3657
SMILES C(=O)(N)NO
Standardized SMILES NC(=O)NO
Molecular weight 76.0547
ALogP -1.03
H-bond donor count 3
H-bond acceptor count 2
Response signature heme biosynthesis & mitochondrial translocase

Pool Growth Kinetics
% growth inhibition (Het. pool) 14.35
% growth inhibition (Hom. pool) 6.7


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3657
Download HIP data (tab-delimited text)  (excel)
Gene:ACS2(YLR153C)|FD-Score:6.23|P-value:2.33E-10|Clearance:0.37||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions Gene:BET4(YJL031C)|FD-Score:4.81|P-value:7.55E-7|Clearance:0.13||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:CDC31(YOR257W)|FD-Score:3.19|P-value:7.05E-4|Clearance:0.12||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:DBP2(YNL112W)|FD-Score:5.73|P-value:4.89E-9|Clearance:0.32||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:ERG7(YHR072W)|FD-Score:4.63|P-value:1.84E-6|Clearance:0.04||SGD DESC:Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis Gene:HEM12(YDR047W)|FD-Score:49.8|P-value:0|Clearance:41.06||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:IMP4(YNL075W)|FD-Score:4.01|P-value:3.08E-5|Clearance:0.1||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing; interacts with Mpp10p; member of a superfamily of proteins that contain a sigma(70)-like motif and associate with RNAs Gene:KOG1(YHR186C)|FD-Score:5.15|P-value:1.30E-7|Clearance:0.07||SGD DESC:Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors Gene:MAK16(YAL025C)|FD-Score:3.48|P-value:2.52E-4|Clearance:0.04||SGD DESC:Essential nuclear protein, constituent of 66S pre-ribosomal particles; required for maturation of 25S and 5.8S rRNAs; required for maintenance of M1 satellite double-stranded RNA of the L-A virus Gene:NOP15(YNL110C)|FD-Score:5.28|P-value:6.55E-8|Clearance:0.12||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:PDS1(YDR113C)|FD-Score:4.42|P-value:4.83E-6|Clearance:0.18||SGD DESC:Securin, inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:PRE4(YFR050C)|FD-Score:4.25|P-value:1.08E-5|Clearance:0.24||SGD DESC:Beta 7 subunit of the 20S proteasome Gene:PRP5(YBR237W)|FD-Score:5.15|P-value:1.28E-7|Clearance:0||SGD DESC:RNA helicase in the DEAD-box family, necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA Gene:RAT1(YOR048C)|FD-Score:3.67|P-value:1.23E-4|Clearance:0.07||SGD DESC:Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination Gene:RLI1(YDR091C)|FD-Score:4.59|P-value:2.19E-6|Clearance:0.17||SGD DESC:Essential iron-sulfur protein required for ribosome biogenesis and translation initiation and termination; facilitates binding of a multifactor complex (MFC) of initiation factors to the small ribosomal subunit; predicted ABC family ATPase; forms a complex with Lto1p and Yae1p that may be involved in protection of the ribosome from damage due to reactive oxygen species Gene:RNR2(YJL026W)|FD-Score:3.9|P-value:4.84E-5|Clearance:0.04||SGD DESC:Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits Gene:RPC17(YJL011C)|FD-Score:3.43|P-value:2.97E-4|Clearance:0.02||SGD DESC:RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress Gene:RPN5(YDL147W)|FD-Score:3.23|P-value:6.09E-4|Clearance:0.04||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RPT3(YDR394W)|FD-Score:5.41|P-value:3.12E-8|Clearance:0.06||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B Gene:RPT5(YOR117W)|FD-Score:5.86|P-value:2.36E-9|Clearance:0.12||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 Gene:RSC58(YLR033W)|FD-Score:7.61|P-value:1.36E-14|Clearance:1.38||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC8(YFR037C)|FD-Score:4.68|P-value:1.44E-6|Clearance:0.01||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:SCC2(YDR180W)|FD-Score:3.41|P-value:3.19E-4|Clearance:0.14||SGD DESC:Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; evolutionarily-conserved adherin Gene:SFH1(YLR321C)|FD-Score:3.86|P-value:5.70E-5|Clearance:0.16||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:SLY1(YDR189W)|FD-Score:3.48|P-value:2.51E-4|Clearance:0||SGD DESC:Hydrophilic protein involved in vesicle trafficking between the ER and Golgi; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex Gene:SPB4(YFL002C)|FD-Score:3.28|P-value:5.22E-4|Clearance:0.04||SGD DESC:Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients Gene:TIF35(YDR429C)|FD-Score:5.09|P-value:1.83E-7|Clearance:0.01||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TIM54(YJL054W)|FD-Score:3.59|P-value:1.63E-4|Clearance:0.11||SGD DESC:Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane Gene:TOM22(YNL131W)|FD-Score:5.08|P-value:1.92E-7|Clearance:0.27||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:UBC9(YDL064W)|FD-Score:5.35|P-value:4.31E-8|Clearance:0.08||SGD DESC:SUMO-conjugating enzyme involved in the Smt3p conjugation pathway; nuclear protein required for S- and M-phase cyclin degradation and mitotic control; involved in proteolysis mediated by the anaphase-promoting complex cyclosome (APCC) Gene:UTP9(YHR196W)|FD-Score:3.7|P-value:1.08E-4|Clearance:0.03||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YDL163W(YDL163W_d)|FD-Score:4.67|P-value:1.49E-6|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene CDC9/YDL164C encoding DNA ligase Gene:YGR265W(YGR265W_d)|FD-Score:3.91|P-value:4.67E-5|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase Gene:YNL114C(YNL114C_d)|FD-Score:8.71|P-value:1.52E-18|Clearance:1.1||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit Gene:ACS2(YLR153C)|FD-Score:6.23|P-value:2.33E-10|Clearance:0.37||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions Gene:BET4(YJL031C)|FD-Score:4.81|P-value:7.55E-7|Clearance:0.13||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:CDC31(YOR257W)|FD-Score:3.19|P-value:7.05E-4|Clearance:0.12||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:DBP2(YNL112W)|FD-Score:5.73|P-value:4.89E-9|Clearance:0.32||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:ERG7(YHR072W)|FD-Score:4.63|P-value:1.84E-6|Clearance:0.04||SGD DESC:Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis Gene:HEM12(YDR047W)|FD-Score:49.8|P-value:0|Clearance:41.06||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:IMP4(YNL075W)|FD-Score:4.01|P-value:3.08E-5|Clearance:0.1||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing; interacts with Mpp10p; member of a superfamily of proteins that contain a sigma(70)-like motif and associate with RNAs Gene:KOG1(YHR186C)|FD-Score:5.15|P-value:1.30E-7|Clearance:0.07||SGD DESC:Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors Gene:MAK16(YAL025C)|FD-Score:3.48|P-value:2.52E-4|Clearance:0.04||SGD DESC:Essential nuclear protein, constituent of 66S pre-ribosomal particles; required for maturation of 25S and 5.8S rRNAs; required for maintenance of M1 satellite double-stranded RNA of the L-A virus Gene:NOP15(YNL110C)|FD-Score:5.28|P-value:6.55E-8|Clearance:0.12||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:PDS1(YDR113C)|FD-Score:4.42|P-value:4.83E-6|Clearance:0.18||SGD DESC:Securin, inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:PRE4(YFR050C)|FD-Score:4.25|P-value:1.08E-5|Clearance:0.24||SGD DESC:Beta 7 subunit of the 20S proteasome Gene:PRP5(YBR237W)|FD-Score:5.15|P-value:1.28E-7|Clearance:0||SGD DESC:RNA helicase in the DEAD-box family, necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA Gene:RAT1(YOR048C)|FD-Score:3.67|P-value:1.23E-4|Clearance:0.07||SGD DESC:Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination Gene:RLI1(YDR091C)|FD-Score:4.59|P-value:2.19E-6|Clearance:0.17||SGD DESC:Essential iron-sulfur protein required for ribosome biogenesis and translation initiation and termination; facilitates binding of a multifactor complex (MFC) of initiation factors to the small ribosomal subunit; predicted ABC family ATPase; forms a complex with Lto1p and Yae1p that may be involved in protection of the ribosome from damage due to reactive oxygen species Gene:RNR2(YJL026W)|FD-Score:3.9|P-value:4.84E-5|Clearance:0.04||SGD DESC:Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits Gene:RPC17(YJL011C)|FD-Score:3.43|P-value:2.97E-4|Clearance:0.02||SGD DESC:RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress Gene:RPN5(YDL147W)|FD-Score:3.23|P-value:6.09E-4|Clearance:0.04||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RPT3(YDR394W)|FD-Score:5.41|P-value:3.12E-8|Clearance:0.06||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B Gene:RPT5(YOR117W)|FD-Score:5.86|P-value:2.36E-9|Clearance:0.12||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 Gene:RSC58(YLR033W)|FD-Score:7.61|P-value:1.36E-14|Clearance:1.38||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC8(YFR037C)|FD-Score:4.68|P-value:1.44E-6|Clearance:0.01||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:SCC2(YDR180W)|FD-Score:3.41|P-value:3.19E-4|Clearance:0.14||SGD DESC:Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; evolutionarily-conserved adherin Gene:SFH1(YLR321C)|FD-Score:3.86|P-value:5.70E-5|Clearance:0.16||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:SLY1(YDR189W)|FD-Score:3.48|P-value:2.51E-4|Clearance:0||SGD DESC:Hydrophilic protein involved in vesicle trafficking between the ER and Golgi; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex Gene:SPB4(YFL002C)|FD-Score:3.28|P-value:5.22E-4|Clearance:0.04||SGD DESC:Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients Gene:TIF35(YDR429C)|FD-Score:5.09|P-value:1.83E-7|Clearance:0.01||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TIM54(YJL054W)|FD-Score:3.59|P-value:1.63E-4|Clearance:0.11||SGD DESC:Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane Gene:TOM22(YNL131W)|FD-Score:5.08|P-value:1.92E-7|Clearance:0.27||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:UBC9(YDL064W)|FD-Score:5.35|P-value:4.31E-8|Clearance:0.08||SGD DESC:SUMO-conjugating enzyme involved in the Smt3p conjugation pathway; nuclear protein required for S- and M-phase cyclin degradation and mitotic control; involved in proteolysis mediated by the anaphase-promoting complex cyclosome (APCC) Gene:UTP9(YHR196W)|FD-Score:3.7|P-value:1.08E-4|Clearance:0.03||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YDL163W(YDL163W_d)|FD-Score:4.67|P-value:1.49E-6|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene CDC9/YDL164C encoding DNA ligase Gene:YGR265W(YGR265W_d)|FD-Score:3.91|P-value:4.67E-5|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase Gene:YNL114C(YNL114C_d)|FD-Score:8.71|P-value:1.52E-18|Clearance:1.1||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3657
Download HOP data (tab-delimited text)  (excel)
Gene:AFT1(YGL071W)|FD-Score:8.69|P-value:1.74E-18||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AHC2(YCR082W)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex Gene:BPH1(YCR032W)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Protein homologous to Chediak-Higashi syndrome and Beige proteins; both of which are implicated in disease syndromes in human and mouse, respectively, due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting Gene:BSC5(YNR069C)|FD-Score:3.21|P-value:6.59E-4||SGD DESC:Protein of unknown function; shows homology with N-terminal end of Bul1p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough expression includes YNR068C and the locus for this readthrough is termed BUL3; Bul3p is involved in ubiquitin-mediated sorting of plasma membrane proteins; readthrough and shortened forms of Bul3p interact with Rsp5p differently in vitro Gene:CAD1(YDR423C)|FD-Score:5.29|P-value:6.05E-8||SGD DESC:AP-1-like basic leucine zipper (bZIP) transcriptional activator; involved in stress responses, iron metabolism, and pleiotropic drug resistance; controls a set of genes involved in stabilizing proteins; binds consensus sequence TTACTAA; CAD1 has a paralog, YAP1, that arose from the whole genome duplication Gene:COT1(YOR316C)|FD-Score:3.21|P-value:6.73E-4||SGD DESC:Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistance to cobalt and rhodium; protein abundance increases in response to DNA replication stress; COT1 has a paralog, ZRC1, that arose from the whole genome duplication Gene:CSM1(YCR086W)|FD-Score:7.78|P-value:3.67E-15||SGD DESC:Nucleolar protein that forms a complex with Lrs4p and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:DAP1(YPL170W)|FD-Score:8.7|P-value:1.67E-18||SGD DESC:Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis Gene:DAT1(YML113W)|FD-Score:-3.17|P-value:7.55E-4||SGD DESC:DNA binding protein that recognizes oligo(dA).oligo(dT) tracts; Arg side chain in its N-terminal pentad Gly-Arg-Lys-Pro-Gly repeat is required for DNA-binding; not essential for viability Gene:DOA4(YDR069C)|FD-Score:4.69|P-value:1.36E-6||SGD DESC:Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole Gene:DUN1(YDL101C)|FD-Score:6.02|P-value:8.87E-10||SGD DESC:Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair Gene:ECM4(YKR076W)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:Omega class glutathione transferase; not essential; similar to Ygr154cp; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:ELM1(YKL048C)|FD-Score:3.11|P-value:9.32E-4||SGD DESC:Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases, such as Kin4p; forms part of the bud neck ring Gene:FET4(YMR319C)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FMP48(YGR052W_p)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FUS2(YMR232W)|FD-Score:3.85|P-value:5.80E-5||SGD DESC:Cytoplasmic protein localized to the shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating Gene:GEA2(YEL022W)|FD-Score:-3.25|P-value:5.80E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:GET2(YER083C)|FD-Score:4.67|P-value:1.51E-6||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GLR1(YPL091W)|FD-Score:4.38|P-value:5.88E-6||SGD DESC:Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress Gene:HMT1(YBR034C)|FD-Score:3.91|P-value:4.65E-5||SGD DESC:Nuclear SAM-dependent mono- and asymmetric arginine dimethylating methyltransferase that modifies hnRNPs, including Npl3p and Hrp1p, affecting their activity and nuclear export; methylates U1 snRNP protein Snp1p and ribosomal protein Rps2p; interacts genetically with genes encoding components of Rpd3(L) and this interaction is important for Rpd3 recruitment to the subtelomeric region. Gene:HNT3(YOR258W)|FD-Score:3.89|P-value:5.02E-5||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:HPF1(YOL155C)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Haze-protective mannoprotein that reduces the particle size of aggregated proteins in white wines Gene:HSL7(YBR133C)|FD-Score:6.38|P-value:9.00E-11||SGD DESC:Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress Gene:IRC4(YDR540C)|FD-Score:4.03|P-value:2.85E-5||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:JEM1(YJL073W)|FD-Score:4.51|P-value:3.21E-6||SGD DESC:DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 Gene:LSM1(YJL124C)|FD-Score:4.23|P-value:1.14E-5||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:MAM1(YER106W)|FD-Score:4.16|P-value:1.60E-5||SGD DESC:Monopolin, kinetochore associated protein involved in chromosome attachment to meiotic spindle Gene:MMS4(YBR098W)|FD-Score:4.27|P-value:9.57E-6||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:NPT1(YOR209C)|FD-Score:4.41|P-value:5.17E-6||SGD DESC:Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus Gene:NTA1(YJR062C)|FD-Score:-3.18|P-value:7.32E-4||SGD DESC:Amidase, removes the amide group from N-terminal asparagine and glutamine residues to generate proteins with N-terminal aspartate and glutamate residues that are targets of ubiquitin-mediated degradation Gene:NVJ1(YHR195W)|FD-Score:4.42|P-value:4.85E-6||SGD DESC:Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN) Gene:OTU2(YHL013C)|FD-Score:5.25|P-value:7.55E-8||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; member of the ovarian tumor-like (OTU) superfamily of predicted cysteine proteases; shows cytoplasmic localization; protein abundance increases in response to DNA replication stress Gene:PCL1(YNL289W)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; is ubiquitinated by Dma1p; phosphorylation by Pho85p targets it for degradation; localizes to sites of polarized cell growth Gene:PKP2(YGL059W)|FD-Score:-3.23|P-value:6.26E-4||SGD DESC:Mitochondrial protein kinase; negatively regulates activity of the pyruvate dehydrogenase complex by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp1p and phosphatases Ptc5p and Ptc6p; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:PRO2(YOR323C)|FD-Score:7.51|P-value:2.93E-14||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PRY3(YJL078C)|FD-Score:3.37|P-value:3.83E-4||SGD DESC:Cell wall-associated protein involved in export of acetylated sterols; member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); role in mating efficiency; expression of full-length transcript is daughter cell-specific; in response to alpha factor, a short transcript starting at +452 is expressed and the long form is repressed by Ste12p Gene:RAD27(YKL113C)|FD-Score:4.95|P-value:3.72E-7||SGD DESC:5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family Gene:RAD54(YGL163C)|FD-Score:4.23|P-value:1.15E-5||SGD DESC:DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress Gene:RAD55(YDR076W)|FD-Score:4.12|P-value:1.92E-5||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p Gene:RAD57(YDR004W)|FD-Score:8.06|P-value:3.72E-16||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p Gene:RNR4(YGR180C)|FD-Score:5.58|P-value:1.20E-8||SGD DESC:Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:ROT2(YBR229C)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations Gene:RPB9(YGL070C)|FD-Score:3.99|P-value:3.25E-5||SGD DESC:RNA polymerase II subunit B12.6; contacts DNA; mutations affect transcription start site selection and fidelity of transcription Gene:RPS7A(YOR096W)|FD-Score:4.54|P-value:2.83E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7A has a paralog, RPS7B, that arose from the whole genome duplication Gene:SCJ1(YMR214W)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:One of several homologs of bacterial chaperone DnaJ, located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins Gene:SCW11(YGL028C)|FD-Score:3.98|P-value:3.38E-5||SGD DESC:Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on its regulation by Ste12p Gene:SPC72(YAL047C)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization Gene:STO1(YMR125W)|FD-Score:3.15|P-value:8.06E-4||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:STP1(YDR463W)|FD-Score:3.32|P-value:4.51E-4||SGD DESC:Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication Gene:SWI3(YJL176C)|FD-Score:4.6|P-value:2.15E-6||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; SWI/SNF regulates transcription by remodeling chromosomes; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; relocates to the cytosol under hypoxic conditions Gene:TIM11(YDR322C-A)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Subunit e of mitochondrial F1F0-ATPase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; essential for the dimeric and oligomeric state of ATP synthase Gene:TRP2(YER090W)|FD-Score:-3.75|P-value:8.84E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:-3.69|P-value:1.13E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TYW1(YPL207W)|FD-Score:6.8|P-value:5.17E-12||SGD DESC:Iron-sulfer protein required for synthesis of Wybutosine modified tRNA; Wybutosine is a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions; induction by Yap5p in response to iron provides protection from high iron toxicity; overexpression results in increased cellular iron Gene:UGX2(YDL169C)|FD-Score:3.19|P-value:7.22E-4||SGD DESC:Protein of unknown function, transcript accumulates in response to any combination of stress conditions Gene:UME6(YDR207C)|FD-Score:4.99|P-value:3.07E-7||SGD DESC:Key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rpd3p Gene:VID22(YLR373C)|FD-Score:4.1|P-value:2.03E-5||SGD DESC:Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles Gene:YBL083C(YBL083C_d)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 Gene:YCR015C(YCR015C_p)|FD-Score:-3.87|P-value:5.35E-5||SGD DESC:Putative protein of unknown function; YCR015C is not an essential gene Gene:YDR431W(YDR431W_d)|FD-Score:4.45|P-value:4.26E-6||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR174W-A(YGR174W-A_p)|FD-Score:-3.75|P-value:8.87E-5||SGD DESC:Putative protein of unknown function; predicted to have a role in cell budding based on computational guilt by association analysis Gene:YGR273C(YGR273C_p)|FD-Score:3.16|P-value:7.95E-4||SGD DESC:Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene Gene:YHR045W(YHR045W_p)|FD-Score:8.37|P-value:2.98E-17||SGD DESC:Putative protein of unknown function; possible role in iron metabolism and/or amino acid and carbohydrate metabolism; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum Gene:YJL027C(YJL027C_p)|FD-Score:32.7|P-value:3.91E-234||SGD DESC:Putative protein of unknown function Gene:YJL119C(YJL119C_d)|FD-Score:-3.31|P-value:4.67E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKR051W(YKR051W_p)|FD-Score:3.13|P-value:8.80E-4||SGD DESC:Putative protein of unknown function Gene:YME2(YMR302C)|FD-Score:4.7|P-value:1.32E-6||SGD DESC:Integral inner mitochondrial membrane protein with a role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases Gene:YNL109W(YNL109W_d)|FD-Score:3.13|P-value:8.65E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YNL108C Gene:YOL097W-A(YOL097W-A_p)|FD-Score:-3.82|P-value:6.59E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YOR059C(YOR059C_p)|FD-Score:3.24|P-value:5.96E-4||SGD DESC:Lipid particle protein of unknown function; contains a putative lipase serine active site; induced by transcription factor Rpn4p; protein abundance increases in response to DNA replication stress Gene:YOR309C(YOR309C_d)|FD-Score:5.38|P-value:3.66E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58 Gene:YPR097W(YPR097W)|FD-Score:3.81|P-value:6.90E-5||SGD DESC:Protein that contains a Phox homology (PX) domain and binds phosphoinositides; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPR123C(YPR123C_d)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPR197C(YPR197C_d)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:AFT1(YGL071W)|FD-Score:8.69|P-value:1.74E-18||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AHC2(YCR082W)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex Gene:BPH1(YCR032W)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Protein homologous to Chediak-Higashi syndrome and Beige proteins; both of which are implicated in disease syndromes in human and mouse, respectively, due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting Gene:BSC5(YNR069C)|FD-Score:3.21|P-value:6.59E-4||SGD DESC:Protein of unknown function; shows homology with N-terminal end of Bul1p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough expression includes YNR068C and the locus for this readthrough is termed BUL3; Bul3p is involved in ubiquitin-mediated sorting of plasma membrane proteins; readthrough and shortened forms of Bul3p interact with Rsp5p differently in vitro Gene:CAD1(YDR423C)|FD-Score:5.29|P-value:6.05E-8||SGD DESC:AP-1-like basic leucine zipper (bZIP) transcriptional activator; involved in stress responses, iron metabolism, and pleiotropic drug resistance; controls a set of genes involved in stabilizing proteins; binds consensus sequence TTACTAA; CAD1 has a paralog, YAP1, that arose from the whole genome duplication Gene:COT1(YOR316C)|FD-Score:3.21|P-value:6.73E-4||SGD DESC:Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistance to cobalt and rhodium; protein abundance increases in response to DNA replication stress; COT1 has a paralog, ZRC1, that arose from the whole genome duplication Gene:CSM1(YCR086W)|FD-Score:7.78|P-value:3.67E-15||SGD DESC:Nucleolar protein that forms a complex with Lrs4p and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:DAP1(YPL170W)|FD-Score:8.7|P-value:1.67E-18||SGD DESC:Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis Gene:DAT1(YML113W)|FD-Score:-3.17|P-value:7.55E-4||SGD DESC:DNA binding protein that recognizes oligo(dA).oligo(dT) tracts; Arg side chain in its N-terminal pentad Gly-Arg-Lys-Pro-Gly repeat is required for DNA-binding; not essential for viability Gene:DOA4(YDR069C)|FD-Score:4.69|P-value:1.36E-6||SGD DESC:Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole Gene:DUN1(YDL101C)|FD-Score:6.02|P-value:8.87E-10||SGD DESC:Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair Gene:ECM4(YKR076W)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:Omega class glutathione transferase; not essential; similar to Ygr154cp; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:ELM1(YKL048C)|FD-Score:3.11|P-value:9.32E-4||SGD DESC:Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases, such as Kin4p; forms part of the bud neck ring Gene:FET4(YMR319C)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FMP48(YGR052W_p)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FUS2(YMR232W)|FD-Score:3.85|P-value:5.80E-5||SGD DESC:Cytoplasmic protein localized to the shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating Gene:GEA2(YEL022W)|FD-Score:-3.25|P-value:5.80E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:GET2(YER083C)|FD-Score:4.67|P-value:1.51E-6||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GLR1(YPL091W)|FD-Score:4.38|P-value:5.88E-6||SGD DESC:Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress Gene:HMT1(YBR034C)|FD-Score:3.91|P-value:4.65E-5||SGD DESC:Nuclear SAM-dependent mono- and asymmetric arginine dimethylating methyltransferase that modifies hnRNPs, including Npl3p and Hrp1p, affecting their activity and nuclear export; methylates U1 snRNP protein Snp1p and ribosomal protein Rps2p; interacts genetically with genes encoding components of Rpd3(L) and this interaction is important for Rpd3 recruitment to the subtelomeric region. Gene:HNT3(YOR258W)|FD-Score:3.89|P-value:5.02E-5||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:HPF1(YOL155C)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Haze-protective mannoprotein that reduces the particle size of aggregated proteins in white wines Gene:HSL7(YBR133C)|FD-Score:6.38|P-value:9.00E-11||SGD DESC:Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress Gene:IRC4(YDR540C)|FD-Score:4.03|P-value:2.85E-5||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:JEM1(YJL073W)|FD-Score:4.51|P-value:3.21E-6||SGD DESC:DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 Gene:LSM1(YJL124C)|FD-Score:4.23|P-value:1.14E-5||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:MAM1(YER106W)|FD-Score:4.16|P-value:1.60E-5||SGD DESC:Monopolin, kinetochore associated protein involved in chromosome attachment to meiotic spindle Gene:MMS4(YBR098W)|FD-Score:4.27|P-value:9.57E-6||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:NPT1(YOR209C)|FD-Score:4.41|P-value:5.17E-6||SGD DESC:Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus Gene:NTA1(YJR062C)|FD-Score:-3.18|P-value:7.32E-4||SGD DESC:Amidase, removes the amide group from N-terminal asparagine and glutamine residues to generate proteins with N-terminal aspartate and glutamate residues that are targets of ubiquitin-mediated degradation Gene:NVJ1(YHR195W)|FD-Score:4.42|P-value:4.85E-6||SGD DESC:Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN) Gene:OTU2(YHL013C)|FD-Score:5.25|P-value:7.55E-8||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; member of the ovarian tumor-like (OTU) superfamily of predicted cysteine proteases; shows cytoplasmic localization; protein abundance increases in response to DNA replication stress Gene:PCL1(YNL289W)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; is ubiquitinated by Dma1p; phosphorylation by Pho85p targets it for degradation; localizes to sites of polarized cell growth Gene:PKP2(YGL059W)|FD-Score:-3.23|P-value:6.26E-4||SGD DESC:Mitochondrial protein kinase; negatively regulates activity of the pyruvate dehydrogenase complex by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp1p and phosphatases Ptc5p and Ptc6p; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:PRO2(YOR323C)|FD-Score:7.51|P-value:2.93E-14||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PRY3(YJL078C)|FD-Score:3.37|P-value:3.83E-4||SGD DESC:Cell wall-associated protein involved in export of acetylated sterols; member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); role in mating efficiency; expression of full-length transcript is daughter cell-specific; in response to alpha factor, a short transcript starting at +452 is expressed and the long form is repressed by Ste12p Gene:RAD27(YKL113C)|FD-Score:4.95|P-value:3.72E-7||SGD DESC:5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family Gene:RAD54(YGL163C)|FD-Score:4.23|P-value:1.15E-5||SGD DESC:DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress Gene:RAD55(YDR076W)|FD-Score:4.12|P-value:1.92E-5||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p Gene:RAD57(YDR004W)|FD-Score:8.06|P-value:3.72E-16||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p Gene:RNR4(YGR180C)|FD-Score:5.58|P-value:1.20E-8||SGD DESC:Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:ROT2(YBR229C)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Glucosidase II catalytic subunit required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations Gene:RPB9(YGL070C)|FD-Score:3.99|P-value:3.25E-5||SGD DESC:RNA polymerase II subunit B12.6; contacts DNA; mutations affect transcription start site selection and fidelity of transcription Gene:RPS7A(YOR096W)|FD-Score:4.54|P-value:2.83E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7A has a paralog, RPS7B, that arose from the whole genome duplication Gene:SCJ1(YMR214W)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:One of several homologs of bacterial chaperone DnaJ, located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins Gene:SCW11(YGL028C)|FD-Score:3.98|P-value:3.38E-5||SGD DESC:Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on its regulation by Ste12p Gene:SPC72(YAL047C)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization Gene:STO1(YMR125W)|FD-Score:3.15|P-value:8.06E-4||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:STP1(YDR463W)|FD-Score:3.32|P-value:4.51E-4||SGD DESC:Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication Gene:SWI3(YJL176C)|FD-Score:4.6|P-value:2.15E-6||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; SWI/SNF regulates transcription by remodeling chromosomes; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; relocates to the cytosol under hypoxic conditions Gene:TIM11(YDR322C-A)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Subunit e of mitochondrial F1F0-ATPase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; essential for the dimeric and oligomeric state of ATP synthase Gene:TRP2(YER090W)|FD-Score:-3.75|P-value:8.84E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:-3.69|P-value:1.13E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TYW1(YPL207W)|FD-Score:6.8|P-value:5.17E-12||SGD DESC:Iron-sulfer protein required for synthesis of Wybutosine modified tRNA; Wybutosine is a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions; induction by Yap5p in response to iron provides protection from high iron toxicity; overexpression results in increased cellular iron Gene:UGX2(YDL169C)|FD-Score:3.19|P-value:7.22E-4||SGD DESC:Protein of unknown function, transcript accumulates in response to any combination of stress conditions Gene:UME6(YDR207C)|FD-Score:4.99|P-value:3.07E-7||SGD DESC:Key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rpd3p Gene:VID22(YLR373C)|FD-Score:4.1|P-value:2.03E-5||SGD DESC:Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles Gene:YBL083C(YBL083C_d)|FD-Score:3.31|P-value:4.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 Gene:YCR015C(YCR015C_p)|FD-Score:-3.87|P-value:5.35E-5||SGD DESC:Putative protein of unknown function; YCR015C is not an essential gene Gene:YDR431W(YDR431W_d)|FD-Score:4.45|P-value:4.26E-6||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR174W-A(YGR174W-A_p)|FD-Score:-3.75|P-value:8.87E-5||SGD DESC:Putative protein of unknown function; predicted to have a role in cell budding based on computational guilt by association analysis Gene:YGR273C(YGR273C_p)|FD-Score:3.16|P-value:7.95E-4||SGD DESC:Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene Gene:YHR045W(YHR045W_p)|FD-Score:8.37|P-value:2.98E-17||SGD DESC:Putative protein of unknown function; possible role in iron metabolism and/or amino acid and carbohydrate metabolism; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum Gene:YJL027C(YJL027C_p)|FD-Score:32.7|P-value:3.91E-234||SGD DESC:Putative protein of unknown function Gene:YJL119C(YJL119C_d)|FD-Score:-3.31|P-value:4.67E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKR051W(YKR051W_p)|FD-Score:3.13|P-value:8.80E-4||SGD DESC:Putative protein of unknown function Gene:YME2(YMR302C)|FD-Score:4.7|P-value:1.32E-6||SGD DESC:Integral inner mitochondrial membrane protein with a role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases Gene:YNL109W(YNL109W_d)|FD-Score:3.13|P-value:8.65E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YNL108C Gene:YOL097W-A(YOL097W-A_p)|FD-Score:-3.82|P-value:6.59E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YOR059C(YOR059C_p)|FD-Score:3.24|P-value:5.96E-4||SGD DESC:Lipid particle protein of unknown function; contains a putative lipase serine active site; induced by transcription factor Rpn4p; protein abundance increases in response to DNA replication stress Gene:YOR309C(YOR309C_d)|FD-Score:5.38|P-value:3.66E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58 Gene:YPR097W(YPR097W)|FD-Score:3.81|P-value:6.90E-5||SGD DESC:Protein that contains a Phox homology (PX) domain and binds phosphoinositides; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPR123C(YPR123C_d)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPR197C(YPR197C_d)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR047W49.80041.10HEM12Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
YNL114C_d8.711.52E-181.10YNL114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit
YLR033W7.611.36E-141.38RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YLR153C6.232.33E-100.37ACS2Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions
YOR117W5.862.36E-90.12RPT5One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1
YNL112W5.734.89E-90.32DBP2ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites
YDR394W5.413.12E-80.06RPT3One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B
YDL064W5.354.31E-80.08UBC9SUMO-conjugating enzyme involved in the Smt3p conjugation pathway; nuclear protein required for S- and M-phase cyclin degradation and mitotic control; involved in proteolysis mediated by the anaphase-promoting complex cyclosome (APCC)
YNL110C5.286.55E-80.12NOP15Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm
YBR237W5.151.28E-70.00PRP5RNA helicase in the DEAD-box family, necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA
YHR186C5.151.30E-70.07KOG1Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors
YDR429C5.091.83E-70.01TIF35eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation
YNL131W5.081.92E-70.27TOM22Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes
YJL031C4.817.55E-70.13BET4Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p
YFR037C4.681.44E-60.01RSC8Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YJL027C_p32.703.91E-234YJL027C_pPutative protein of unknown function
YPL170W8.701.67E-18DAP1Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis
YGL071W8.691.74E-18AFT1Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
YHR045W_p8.372.98E-17YHR045W_pPutative protein of unknown function; possible role in iron metabolism and/or amino acid and carbohydrate metabolism; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum
YDR004W8.063.72E-16RAD57Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p
YCR086W7.783.67E-15CSM1Nucleolar protein that forms a complex with Lrs4p and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation
YOR323C7.512.93E-14PRO2Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis
YPL207W6.805.17E-12TYW1Iron-sulfer protein required for synthesis of Wybutosine modified tRNA; Wybutosine is a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions; induction by Yap5p in response to iron provides protection from high iron toxicity; overexpression results in increased cellular iron
YBR133C6.389.00E-11HSL7Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress
YDL101C6.028.87E-10DUN1Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair
YGR180C5.581.20E-8RNR4Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress
YOR309C_d5.383.66E-8YOR309C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58
YDR423C5.296.05E-8CAD1AP-1-like basic leucine zipper (bZIP) transcriptional activator; involved in stress responses, iron metabolism, and pleiotropic drug resistance; controls a set of genes involved in stabilizing proteins; binds consensus sequence TTACTAA; CAD1 has a paralog, YAP1, that arose from the whole genome duplication
YHL013C5.257.55E-8OTU2Protein of unknown function; may interact with ribosomes, based on co-purification experiments; member of the ovarian tumor-like (OTU) superfamily of predicted cysteine proteases; shows cytoplasmic localization; protein abundance increases in response to DNA replication stress
YDR207C4.993.07E-7UME6Key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rpd3p

GO enrichment analysis for SGTC_273
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.4151.30E-240SGTC_10258-0098 57.4 μMChemDiv (Drug-like library)67900320.0384615heme biosynthesis & mitochondrial translocase
0.2404.07E-78SGTC_2617dihydrotanshinone i 20.8 μMMicrosource (Natural product library)53167430.0204082copper-dependent oxidative stress
0.2332.42E-73SGTC_203k066-0004 30.9 μMChemDiv (Drug-like library)67954120.0196078RSC & ERG11
0.2189.18E-65SGTC_23369001819 198.7 μMChembridge (Fragment library)29896020.025641heme biosynthesis & mitochondrial translocase
0.2163.99E-63SGTC_1113artemisinin 1.2 μMTimTec (Natural product library)22400.0208333heme biosynthesis & mitochondrial translocase
0.2054.78E-57SGTC_10573448-5381 134.0 μMChemDiv (Drug-like library)5497911NAcalcium & mitochondrial duress
0.2002.79E-54SGTC_260302-0163 425.2 μMChemDiv (Drug-like library)31009150.0731707heme biosynthesis & mitochondrial translocase
0.1982.96E-53SGTC_8420271-0008 11.1 μMChemDiv (Drug-like library)12217090.027027heme biosynthesis & mitochondrial translocase
0.1931.36E-50SGTC_2519cryptotanshinone 88.0 μMMicrosource (Natural product library)1602540.0188679heme biosynthesis & mitochondrial translocase
0.1755.32E-42SGTC_1556plumbagin 1.5 μMTimTec (Pure natural product library)102050.0555556superoxide
0.1695.57E-39SGTC_208k072-0098 12.4 μMChemDiv (Drug-like library)59489010.0238095heme biosynthesis & mitochondrial translocase
0.1627.30E-36SGTC_1098clofazimine 15.1 μMNIH Clinical Collection27940.0166667heme biosynthesis & mitochondrial translocase
0.1584.37E-34SGTC_623k072-0108 16.9 μMChemDiv (Drug-like library)53828030.0222222heme biosynthesis & mitochondrial translocase
0.1576.03E-34SGTC_2129girgensonine 121.1 μMChembridge (Fragment library)4426380.0263158heme biosynthesis & mitochondrial translocase
0.1576.17E-34SGTC_20375169046 200.0 μMChembridge (Fragment library)57180220.073170760S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1564D-Arginine115 μM0.22580671070TimTec (Pure natural product library)174.20096-3.83144
SGTC_20305175110200 μM0.1764712831986Chembridge (Fragment library)188.182721.20523Golgi
SGTC_1791273-00636.77 μM0.1753108139ChemDiv (Drug-like library)366.56126.93522
SGTC_2261,3-diallylurea8.29 mM0.17391374537Miscellaneous140.182980.60321
SGTC_1050111-0055734.78 μM0.1724145179370ChemDiv (Drug-like library)272.181422.45511
SGTC_9272425-1185123 μM0.166667730375ChemDiv (Drug-like library)316.353243.13522
SGTC_1543azetidyl-2-carboxylic acid198 μM0.16666716486TimTec (Pure natural product library)101.10388-3.42323
SGTC_1796st049173110.4 μM0.16666794134TimTec (Natural product derivative library)181.18854-2.43734
SGTC_2274794709446.08 μM0.1666672971028Chembridge (Fragment library)246.2851.40323
SGTC_1704st02880570.8 μM0.1627912876101TimTec (Natural product derivative library)282.358622.5724