amoxapine FDA approved compound

8-chloro-6-piperazin-1-ylbenzo[b][1,4]benzoxazepine

A moderate and strong reuptake inhibitor of serotonin and norepinephrine.

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 

PubChem MeSH terms: Adrenergic Uptake Inhibitors;Antidepressive Agents, Second-Generation;Dopamine Antagonists;Neurotransmitter Uptake Inhibitors;Serotonin Uptake Inhibitors



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2731
Screen concentration 43.4 μM
Source Miscellaneous
PubChem CID 2170
SMILES C1CN(CCN1)C2=NC3=CC=CC=C3OC4=C2C=C(C=C4)Cl
Standardized SMILES Clc1ccc2Oc3ccccc3N=C(N4CCNCC4)c2c1
Molecular weight 313.7814
ALogP 2.9
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 2.86
% growth inhibition (Hom. pool) 2.62


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2170
Download HIP data (tab-delimited text)  (excel)
Gene:CCL1(YPR025C)|FD-Score:3.21|P-value:6.64E-4|Clearance:0.25||SGD DESC:Cyclin associated with protein kinase Kin28p, which is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters Gene:CWC2(YDL209C)|FD-Score:3.29|P-value:5.08E-4|Clearance:0.08||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:DIS3(YOL021C)|FD-Score:3.39|P-value:3.51E-4|Clearance:0.1||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:MES1(YGR264C)|FD-Score:4.82|P-value:7.10E-7|Clearance:1.02||SGD DESC:Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs Gene:PSA1(YDL055C)|FD-Score:3.78|P-value:7.95E-5|Clearance:0.39||SGD DESC:GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from GTP and mannose-1-phosphate in cell wall biosynthesis; required for normal cell wall structure Gene:RPA43(YOR340C)|FD-Score:3.8|P-value:7.29E-5|Clearance:0.02||SGD DESC:RNA polymerase I subunit A43 Gene:CCL1(YPR025C)|FD-Score:3.21|P-value:6.64E-4|Clearance:0.25||SGD DESC:Cyclin associated with protein kinase Kin28p, which is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters Gene:CWC2(YDL209C)|FD-Score:3.29|P-value:5.08E-4|Clearance:0.08||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:DIS3(YOL021C)|FD-Score:3.39|P-value:3.51E-4|Clearance:0.1||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:MES1(YGR264C)|FD-Score:4.82|P-value:7.10E-7|Clearance:1.02||SGD DESC:Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs Gene:PSA1(YDL055C)|FD-Score:3.78|P-value:7.95E-5|Clearance:0.39||SGD DESC:GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from GTP and mannose-1-phosphate in cell wall biosynthesis; required for normal cell wall structure Gene:RPA43(YOR340C)|FD-Score:3.8|P-value:7.29E-5|Clearance:0.02||SGD DESC:RNA polymerase I subunit A43

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2170
Download HOP data (tab-delimited text)  (excel)
Gene:DIG2(YDR480W)|FD-Score:-3.13|P-value:8.66E-4||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:ERV14(YGL054C)|FD-Score:3.17|P-value:7.54E-4||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:MSD1(YPL104W)|FD-Score:3.22|P-value:6.33E-4||SGD DESC:Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene Gene:PEX17(YNL214W)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Peroxisomal membrane peroxin and subunit of the docking complex that facilitates the import of peroxisomal matrix proteins; required for peroxisome biogenesis Gene:YLL007C(YLL007C_p)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YNL140C(YNL140C_p)|FD-Score:-3.24|P-value:6.02E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps THO2/YNL139C Gene:DIG2(YDR480W)|FD-Score:-3.13|P-value:8.66E-4||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:ERV14(YGL054C)|FD-Score:3.17|P-value:7.54E-4||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:MSD1(YPL104W)|FD-Score:3.22|P-value:6.33E-4||SGD DESC:Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene Gene:PEX17(YNL214W)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Peroxisomal membrane peroxin and subunit of the docking complex that facilitates the import of peroxisomal matrix proteins; required for peroxisome biogenesis Gene:YLL007C(YLL007C_p)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YNL140C(YNL140C_p)|FD-Score:-3.24|P-value:6.02E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps THO2/YNL139C

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGR264C4.827.10E-71.02MES1Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs
YOR340C3.807.29E-50.02RPA43RNA polymerase I subunit A43
YDL055C3.787.95E-50.39PSA1GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from GTP and mannose-1-phosphate in cell wall biosynthesis; required for normal cell wall structure
YOL021C3.393.51E-40.10DIS3Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress
YDL209C3.295.08E-40.08CWC2Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2
YPR025C3.216.64E-40.25CCL1Cyclin associated with protein kinase Kin28p, which is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters
YBL020W2.960.001540.05RFT1Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein
YJL097W2.910.001780.02PHS1Essential 3-hydroxyacyl-CoA dehydratase of the ER membrane, involved in elongation of very long-chain fatty acids; evolutionarily conserved, similar to mammalian PTPLA and PTPLB; involved in sphingolipid biosynthesis and protein trafficking
YHR019C2.890.001900.01DED81Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA
YDR478W2.890.001930.07SNM1Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP
YDL031W2.820.002390.17DBP10Putative ATP-dependent RNA helicase of the DEAD-box protein family, constituent of 66S pre-ribosomal particles; essential protein involved in ribosome biogenesis
YJR042W2.650.004030.04NUP85Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75
YGL099W2.610.004490.01LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YGL111W2.600.004670.05NSA1Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis
YLR459W2.550.005440.04GAB1GPI transamidase subunit, involved in attachment of glycosylphosphatidylinositol (GPI) anchors to proteins; may have a role in recognition of the attachment signal or of the lipid portion of GPI

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YNL214W3.413.27E-4PEX17Peroxisomal membrane peroxin and subunit of the docking complex that facilitates the import of peroxisomal matrix proteins; required for peroxisome biogenesis
YLL007C_p3.265.58E-4YLL007C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene
YPL104W3.226.33E-4MSD1Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene
YGL054C3.177.54E-4ERV14COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication
YMR179W3.070.00108SPT21Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p; localizes to nuclear foci that become diffuse upon DNA replication stress
YDL066W3.050.00114IDP1Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes
YIL133C2.980.00143RPL16ARibosomal 60S subunit protein L16A; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16A has a paralog, RPL16B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
YPL039W_p2.940.00166YPL039W_pPutative protein of unknown function; YPL039W is not an essential gene
YEL005C2.880.00199VAB2Protein with a potential role in vacuolar function, as suggested by its ability to bind Vac8p; likely member of BLOC complex involved in endosomal cargo sorting; Vab2p-GFP-fusion localizes to cytoplasm in punctate patter
YKL212W2.850.00221SAC1Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism
YBL006C2.800.00256LDB7Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions
YHL020C2.780.00272OPI1Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance
YML060W2.760.00289OGG1Mitochondrial glycosylase/lyase that specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA, contributes to UVA resistance
YNR068C_p2.760.00292YNR068C_pPutative protein of unknown function; exhibits homology to C-terminal end of Bul1p; expressed as a readthrough product of BSC5, the readthrough locus being termed BUL3; the BUL3 readthrough product is involved in ubiquitin-mediated sorting of plasma membrane proteins and interacts with WW domains of Rsp5p in vitro, but in a functionally different way than the non-readthrough form
YPL006W2.690.00360NCR1Vacuolar membrane protein that transits through the biosynthetic vacuolar protein sorting pathway, involved in sphingolipid metabolism; glycoprotein and functional orthologue of human Niemann Pick C1 (NPC1) protein

GO enrichment analysis for SGTC_2731
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1779.61E-43SGTC_2734clemastine 41.6 μMMiscellaneous269870.169014ERG2
0.1701.86E-39SGTC_2732terfenadine 20.0 μMMiscellaneous54050.102564ERG2
0.0984.24E-14SGTC_2714st079313 56.9 μMTimTec (Natural product derivative library)29397990.0789474
0.0898.88E-12SGTC_32579139206 33.0 μMChembridge (Drug-like library)49100410.138889
0.0872.75E-11SGTC_2720fluspirilene 36.9 μMMiscellaneous33960.111111ERG2
0.0863.22E-11SGTC_2724maprotiline 69.6 μMMiscellaneous40110.0869565ERG2
0.0855.16E-11SGTC_32589138477 49.5 μMChembridge (Drug-like library)49703550.142857
0.0807.02E-10SGTC_1558st069300 45.6 μMTimTec (Pure natural product library)242080000.0434783
0.0809.39E-10SGTC_2615-fluorouridine 15.4 μMMiscellaneous18210.0684932exosome
0.0782.05E-9SGTC_28519015922 71.4 μMChembridge (Drug-like library)29968930.0657895
0.0772.68E-9SGTC_1074carmofur 3.8 μMNIH Clinical Collection25770.0533333exosome
0.0741.15E-8SGTC_2646retinol 96.8 μMMicrosource (Natural product library)4453540.0253165
0.0741.27E-8SGTC_5371334-0398 209.0 μMChemDiv (Drug-like library)58469860.0675676
0.0731.65E-8SGTC_9701315-0089 19.5 μMChemDiv (Drug-like library)68110830.0705882RSC & ERG11
0.0723.14E-8SGTC_1820st053765 18.6 μMTimTec (Natural product derivative library)36908290.17284RPP1 & pyrimidine depletion

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2742clothiapine36.27 μM0.43859616351Miscellaneous343.873623.99103NEO1
SGTC_232clozapine119 μM0.4137932818Miscellaneous326.823263.47614NEO1
SGTC_443clozapine122 μM0.4137932818Miscellaneous326.823263.47614NEO1-PIK1
SGTC_235890371574 μM0.3157892388925Chembridge (Fragment library)231.250520.20513
SGTC_3393091-483371.4 μM0.3050853240077ChemDiv (Drug-like library)272.686463.1713iron homeostasis
SGTC_12690831-07963.05 μM0.3927758ChemDiv (Drug-like library)268.697763.1170160S ribosome export
SGTC_1723st0343072.55 μM0.290909867466TimTec (Natural product derivative library)297.949563.81402mitochondrial processes
SGTC_1766st04541444.77 μM0.285714253960TimTec (Natural product derivative library)273.671222.11214RPP1 & pyrimidine depletion
SGTC_1780st03844824.54 μM0.274194728868TimTec (Natural product derivative library)278.710883.21614iron homeostasis
SGTC_1896st05844189 μM0.271186688759TimTec (Natural product derivative library)224.640362.603
SGTC_2094077-0173175.59 μM0.26562515993494ChemDiv (Drug-like library)284.740223.53903