miltefosine FDA approved compound

hexadecyl 2-(trimethylazaniumyl)ethyl phosphate

Inhibitor of protein kinase C and of phosphatidylcholine synthesis.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2741
Screen concentration 3.0 μM
Source Miscellaneous
PubChem CID 3599
SMILES CCCCCCCCCCCCCCCCOP(=O)([O-])OCC[N+](C)(C)C
Standardized SMILES CCCCCCCCCCCCCCCCOP(=O)([O-])OCC[N+](C)(C)C
Molecular weight 407.568
ALogP 3.72
H-bond donor count 0
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 5.87
% growth inhibition (Hom. pool) 1.39


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3599
Download HIP data (tab-delimited text)  (excel)
Gene:CCT7(YJL111W)|FD-Score:4.18|P-value:1.45E-5|Clearance:0.42||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CEP3(YMR168C)|FD-Score:3.76|P-value:8.46E-5|Clearance:0.21||SGD DESC:Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain Gene:MCM1(YMR043W)|FD-Score:-3.79|P-value:7.43E-5|Clearance:0||SGD DESC:Transcription factor involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes Gene:PRI1(YIR008C)|FD-Score:3.55|P-value:1.89E-4|Clearance:0.29||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:RPB8(YOR224C)|FD-Score:-3.64|P-value:1.35E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RSC8(YFR037C)|FD-Score:-3.36|P-value:3.84E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:TIM54(YJL054W)|FD-Score:3.27|P-value:5.42E-4|Clearance:0.05||SGD DESC:Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane Gene:TUB1(YML085C)|FD-Score:3.13|P-value:8.71E-4|Clearance:0.05||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:YDR053W(YDR053W_d)|FD-Score:3.22|P-value:6.38E-4|Clearance:0.09||SGD DESC:Putative protein of unknown function; open reading frame overlaps 5' end of essential DBF4 gene encoding the regulatory subunit of the Cdc7p-Dbf4p kinase complex Gene:YLR230W(YLR230W_d)|FD-Score:-4|P-value:3.14E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity Gene:CCT7(YJL111W)|FD-Score:4.18|P-value:1.45E-5|Clearance:0.42||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CEP3(YMR168C)|FD-Score:3.76|P-value:8.46E-5|Clearance:0.21||SGD DESC:Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain Gene:MCM1(YMR043W)|FD-Score:-3.79|P-value:7.43E-5|Clearance:0||SGD DESC:Transcription factor involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes Gene:PRI1(YIR008C)|FD-Score:3.55|P-value:1.89E-4|Clearance:0.29||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:RPB8(YOR224C)|FD-Score:-3.64|P-value:1.35E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RSC8(YFR037C)|FD-Score:-3.36|P-value:3.84E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:TIM54(YJL054W)|FD-Score:3.27|P-value:5.42E-4|Clearance:0.05||SGD DESC:Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane Gene:TUB1(YML085C)|FD-Score:3.13|P-value:8.71E-4|Clearance:0.05||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:YDR053W(YDR053W_d)|FD-Score:3.22|P-value:6.38E-4|Clearance:0.09||SGD DESC:Putative protein of unknown function; open reading frame overlaps 5' end of essential DBF4 gene encoding the regulatory subunit of the Cdc7p-Dbf4p kinase complex Gene:YLR230W(YLR230W_d)|FD-Score:-4|P-value:3.14E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3599
Download HOP data (tab-delimited text)  (excel)
Gene:ADK1(YDR226W)|FD-Score:6.49|P-value:4.37E-11||SGD DESC:Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress Gene:ARC18(YLR370C)|FD-Score:4.98|P-value:3.16E-7||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARO1(YDR127W)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO3(YDR035W)|FD-Score:4.03|P-value:2.76E-5||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ARP5(YNL059C)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:ASC1(YMR116C)|FD-Score:3.13|P-value:8.88E-4||SGD DESC:G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 that inhibits translation; core component of the small (40S) ribosomal subunit; regulates P-body formation induced by replication stress; represses Gcn4p in the absence of amino acid starvation Gene:BEM3(YPL115C)|FD-Score:3.93|P-value:4.28E-5||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly Gene:BEM4(YPL161C)|FD-Score:4.41|P-value:5.15E-6||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:CCS1(YMR038C)|FD-Score:9.09|P-value:4.97E-20||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CCW12(YLR110C)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CDC73(YLR418C)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:COG8(YML071C)|FD-Score:4.43|P-value:4.65E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTR1(YPR124W)|FD-Score:6.31|P-value:1.42E-10||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:END3(YNL084C)|FD-Score:15.1|P-value:1.35E-51||SGD DESC:EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p Gene:FRK1(YPL141C)|FD-Score:3.17|P-value:7.61E-4||SGD DESC:Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; interacts with rRNA transcription and ribosome biogenesis factors and the long chain fatty acyl-CoA synthetase Faa3p; FRK1 has a paralog, KIN4, that arose from the whole genome duplication Gene:GAL11(YOL051W)|FD-Score:10.8|P-value:1.57E-27||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GAL7(YBR018C)|FD-Score:5.29|P-value:6.15E-8||SGD DESC:Galactose-1-phosphate uridyl transferase, synthesizes glucose-1-phosphate and UDP-galactose from UDP-D-glucose and alpha-D-galactose-1-phosphate in the second step of galactose catabolism Gene:GCN2(YDR283C)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN4(YEL009C)|FD-Score:6.47|P-value:4.86E-11||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GCR2(YNL199C)|FD-Score:6.99|P-value:1.36E-12||SGD DESC:Transcriptional activator of genes involved in glycolysis; interacts and functions with the DNA-binding protein Gcr1p Gene:GIM5(YML094W)|FD-Score:4.17|P-value:1.52E-5||SGD DESC:Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it Gene:HFI1(YPL254W)|FD-Score:7.44|P-value:5.11E-14||SGD DESC:Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions Gene:IBA57(YJR122W)|FD-Score:4.2|P-value:1.35E-5||SGD DESC:Mitochondrial matrix protein involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system Gene:IES2(YNL215W)|FD-Score:4.47|P-value:3.99E-6||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress Gene:INM1(YHR046C)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate Gene:IRC21(YMR073C)|FD-Score:7.4|P-value:7.07E-14||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:LDB17(YDL146W)|FD-Score:3.33|P-value:4.34E-4||SGD DESC:Protein involved in the regulation of endocytosis; transiently recruited to actin cortical patches in a SLA1-dependent manner after late coat component assembly; GFP-fusion protein localizes to the periphery, cytoplasm, bud, and bud neck Gene:LRS4(YDR439W)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:MEH1(YKR007W)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; loss results in a defect in vacuolar acidification Gene:MRPL31(YKL138C)|FD-Score:-3.6|P-value:1.57E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NPT1(YOR209C)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus Gene:OXA1(YER154W)|FD-Score:4.36|P-value:6.56E-6||SGD DESC:Mitochondrial inner membrane insertase, mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane, interacts with mitochondrial ribosomes; conserved from bacteria to animals Gene:PAC10(YGR078C)|FD-Score:3.12|P-value:8.90E-4||SGD DESC:Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding Gene:PGD1(YGL025C)|FD-Score:5.34|P-value:4.64E-8||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:RPN4(YDL020C)|FD-Score:-3.83|P-value:6.53E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RRD1(YIL153W)|FD-Score:7.91|P-value:1.29E-15||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:RSM7(YJR113C)|FD-Score:-3.27|P-value:5.41E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein Gene:RXT2(YBR095C)|FD-Score:4.54|P-value:2.85E-6||SGD DESC:Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth Gene:SBA1(YKL117W)|FD-Score:-3.25|P-value:5.68E-4||SGD DESC:Co-chaperone that binds to and regulates Hsp90 family chaperones; important for pp60v-src activity in yeast; homologous to the mammalian p23 proteins, and like p23 can regulate telomerase activity; protein abundance increases in response to DNA replication stress Gene:SHE4(YOR035C)|FD-Score:3.17|P-value:7.66E-4||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SIT4(YDL047W)|FD-Score:3.82|P-value:6.57E-5||SGD DESC:Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization Gene:SKI8(YGL213C)|FD-Score:-3.65|P-value:1.31E-4||SGD DESC:Ski complex component and WD-repeat protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; also required for meiotic double-strand break recombination; null mutants have superkiller phenotype Gene:SKY1(YMR216C)|FD-Score:9.41|P-value:2.37E-21||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SNF12(YNR023W)|FD-Score:4.24|P-value:1.11E-5||SGD DESC:73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive Gene:SNF2(YOR290C)|FD-Score:12.6|P-value:1.56E-36||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SOD1(YJR104C)|FD-Score:7.43|P-value:5.41E-14||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPR3(YGR059W)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Sporulation-specific homolog of the yeast CDC3/10/11/12 family of bud neck microfilament genes; septin protein involved in sporulation; regulated by ABFI Gene:SRB5(YGR104C)|FD-Score:5.74|P-value:4.63E-9||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance Gene:TDH3(YGR192C)|FD-Score:5.41|P-value:3.14E-8||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; TDH3 has a paralog, TDH2, that arose from the whole genome duplication Gene:TIP41(YPR040W)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress Gene:TRP1(YDR007W)|FD-Score:8.84|P-value:4.82E-19||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:7.29|P-value:1.54E-13||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:7.48|P-value:3.69E-14||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:24.2|P-value:2.10E-129||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:4.87|P-value:5.69E-7||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:UBR1(YGR184C)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:E3 ubiquitin ligase (N-recognin); forms heterodimer with Rad6p to ubiquitinate substrates in the N-end rule pathway; regulates peptide transport via Cup9p ubiquitination; plays a major role in targeting cytosolic misfolded proteins for degradation; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS) Gene:UME6(YDR207C)|FD-Score:6.03|P-value:8.22E-10||SGD DESC:Key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rpd3p Gene:WTM2(YOR229W)|FD-Score:3.22|P-value:6.44E-4||SGD DESC:Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; involved in response to replication stress; contains WD repeats Gene:YAP1802(YGR241C)|FD-Score:3.23|P-value:6.08E-4||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1802 has a paralog, YAP1801, that arose from the whole genome duplication Gene:YDR008C(YDR008C_d)|FD-Score:13.5|P-value:4.33E-42||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR239C(YDR239C)|FD-Score:3.82|P-value:6.81E-5||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YGL117W(YGL117W_p)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Putative protein of unknown function Gene:YIL002W-A(YIL002W-A_p)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YJL120W(YJL120W_d)|FD-Score:4.91|P-value:4.62E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YJL193W(YJL193W_p)|FD-Score:3.16|P-value:7.78E-4||SGD DESC:Putative protein of unknown function, predicted to encode a triose phosphate transporter subfamily member based on phylogenetic analysis; similar to YOR307C/SLY41; deletion mutant has a respiratory growth defect Gene:YLR111W(YLR111W_d)|FD-Score:3.3|P-value:4.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML122C(YML122C_d)|FD-Score:7.9|P-value:1.43E-15||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL198C(YNL198C_d)|FD-Score:5.2|P-value:9.88E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOL050C(YOL050C_d)|FD-Score:4.99|P-value:3.09E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YPR123C(YPR123C_d)|FD-Score:5.15|P-value:1.29E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPR197C(YPR197C_d)|FD-Score:3.15|P-value:8.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YRA2(YKL214C)|FD-Score:3.76|P-value:8.43E-5||SGD DESC:Member of the REF (RNA and export factor binding proteins) family; when overexpressed, can substitute for the function of Yra1p in export of poly(A)+ mRNA from the nucleus Gene:ADK1(YDR226W)|FD-Score:6.49|P-value:4.37E-11||SGD DESC:Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress Gene:ARC18(YLR370C)|FD-Score:4.98|P-value:3.16E-7||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARO1(YDR127W)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO3(YDR035W)|FD-Score:4.03|P-value:2.76E-5||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ARP5(YNL059C)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:ASC1(YMR116C)|FD-Score:3.13|P-value:8.88E-4||SGD DESC:G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 that inhibits translation; core component of the small (40S) ribosomal subunit; regulates P-body formation induced by replication stress; represses Gcn4p in the absence of amino acid starvation Gene:BEM3(YPL115C)|FD-Score:3.93|P-value:4.28E-5||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly Gene:BEM4(YPL161C)|FD-Score:4.41|P-value:5.15E-6||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:CCS1(YMR038C)|FD-Score:9.09|P-value:4.97E-20||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CCW12(YLR110C)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CDC73(YLR418C)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:COG8(YML071C)|FD-Score:4.43|P-value:4.65E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTR1(YPR124W)|FD-Score:6.31|P-value:1.42E-10||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:END3(YNL084C)|FD-Score:15.1|P-value:1.35E-51||SGD DESC:EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p Gene:FRK1(YPL141C)|FD-Score:3.17|P-value:7.61E-4||SGD DESC:Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; interacts with rRNA transcription and ribosome biogenesis factors and the long chain fatty acyl-CoA synthetase Faa3p; FRK1 has a paralog, KIN4, that arose from the whole genome duplication Gene:GAL11(YOL051W)|FD-Score:10.8|P-value:1.57E-27||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GAL7(YBR018C)|FD-Score:5.29|P-value:6.15E-8||SGD DESC:Galactose-1-phosphate uridyl transferase, synthesizes glucose-1-phosphate and UDP-galactose from UDP-D-glucose and alpha-D-galactose-1-phosphate in the second step of galactose catabolism Gene:GCN2(YDR283C)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN4(YEL009C)|FD-Score:6.47|P-value:4.86E-11||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GCR2(YNL199C)|FD-Score:6.99|P-value:1.36E-12||SGD DESC:Transcriptional activator of genes involved in glycolysis; interacts and functions with the DNA-binding protein Gcr1p Gene:GIM5(YML094W)|FD-Score:4.17|P-value:1.52E-5||SGD DESC:Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it Gene:HFI1(YPL254W)|FD-Score:7.44|P-value:5.11E-14||SGD DESC:Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions Gene:IBA57(YJR122W)|FD-Score:4.2|P-value:1.35E-5||SGD DESC:Mitochondrial matrix protein involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system Gene:IES2(YNL215W)|FD-Score:4.47|P-value:3.99E-6||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress Gene:INM1(YHR046C)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate Gene:IRC21(YMR073C)|FD-Score:7.4|P-value:7.07E-14||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:LDB17(YDL146W)|FD-Score:3.33|P-value:4.34E-4||SGD DESC:Protein involved in the regulation of endocytosis; transiently recruited to actin cortical patches in a SLA1-dependent manner after late coat component assembly; GFP-fusion protein localizes to the periphery, cytoplasm, bud, and bud neck Gene:LRS4(YDR439W)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:MEH1(YKR007W)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; loss results in a defect in vacuolar acidification Gene:MRPL31(YKL138C)|FD-Score:-3.6|P-value:1.57E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NPT1(YOR209C)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus Gene:OXA1(YER154W)|FD-Score:4.36|P-value:6.56E-6||SGD DESC:Mitochondrial inner membrane insertase, mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane, interacts with mitochondrial ribosomes; conserved from bacteria to animals Gene:PAC10(YGR078C)|FD-Score:3.12|P-value:8.90E-4||SGD DESC:Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding Gene:PGD1(YGL025C)|FD-Score:5.34|P-value:4.64E-8||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:RPN4(YDL020C)|FD-Score:-3.83|P-value:6.53E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RRD1(YIL153W)|FD-Score:7.91|P-value:1.29E-15||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:RSM7(YJR113C)|FD-Score:-3.27|P-value:5.41E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein Gene:RXT2(YBR095C)|FD-Score:4.54|P-value:2.85E-6||SGD DESC:Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth Gene:SBA1(YKL117W)|FD-Score:-3.25|P-value:5.68E-4||SGD DESC:Co-chaperone that binds to and regulates Hsp90 family chaperones; important for pp60v-src activity in yeast; homologous to the mammalian p23 proteins, and like p23 can regulate telomerase activity; protein abundance increases in response to DNA replication stress Gene:SHE4(YOR035C)|FD-Score:3.17|P-value:7.66E-4||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SIT4(YDL047W)|FD-Score:3.82|P-value:6.57E-5||SGD DESC:Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization Gene:SKI8(YGL213C)|FD-Score:-3.65|P-value:1.31E-4||SGD DESC:Ski complex component and WD-repeat protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; also required for meiotic double-strand break recombination; null mutants have superkiller phenotype Gene:SKY1(YMR216C)|FD-Score:9.41|P-value:2.37E-21||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SNF12(YNR023W)|FD-Score:4.24|P-value:1.11E-5||SGD DESC:73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive Gene:SNF2(YOR290C)|FD-Score:12.6|P-value:1.56E-36||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SOD1(YJR104C)|FD-Score:7.43|P-value:5.41E-14||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPR3(YGR059W)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Sporulation-specific homolog of the yeast CDC3/10/11/12 family of bud neck microfilament genes; septin protein involved in sporulation; regulated by ABFI Gene:SRB5(YGR104C)|FD-Score:5.74|P-value:4.63E-9||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance Gene:TDH3(YGR192C)|FD-Score:5.41|P-value:3.14E-8||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; TDH3 has a paralog, TDH2, that arose from the whole genome duplication Gene:TIP41(YPR040W)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress Gene:TRP1(YDR007W)|FD-Score:8.84|P-value:4.82E-19||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:7.29|P-value:1.54E-13||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:7.48|P-value:3.69E-14||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:24.2|P-value:2.10E-129||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:4.87|P-value:5.69E-7||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:UBR1(YGR184C)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:E3 ubiquitin ligase (N-recognin); forms heterodimer with Rad6p to ubiquitinate substrates in the N-end rule pathway; regulates peptide transport via Cup9p ubiquitination; plays a major role in targeting cytosolic misfolded proteins for degradation; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS) Gene:UME6(YDR207C)|FD-Score:6.03|P-value:8.22E-10||SGD DESC:Key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rpd3p Gene:WTM2(YOR229W)|FD-Score:3.22|P-value:6.44E-4||SGD DESC:Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; involved in response to replication stress; contains WD repeats Gene:YAP1802(YGR241C)|FD-Score:3.23|P-value:6.08E-4||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1802 has a paralog, YAP1801, that arose from the whole genome duplication Gene:YDR008C(YDR008C_d)|FD-Score:13.5|P-value:4.33E-42||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR239C(YDR239C)|FD-Score:3.82|P-value:6.81E-5||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YGL117W(YGL117W_p)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Putative protein of unknown function Gene:YIL002W-A(YIL002W-A_p)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YJL120W(YJL120W_d)|FD-Score:4.91|P-value:4.62E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YJL193W(YJL193W_p)|FD-Score:3.16|P-value:7.78E-4||SGD DESC:Putative protein of unknown function, predicted to encode a triose phosphate transporter subfamily member based on phylogenetic analysis; similar to YOR307C/SLY41; deletion mutant has a respiratory growth defect Gene:YLR111W(YLR111W_d)|FD-Score:3.3|P-value:4.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML122C(YML122C_d)|FD-Score:7.9|P-value:1.43E-15||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL198C(YNL198C_d)|FD-Score:5.2|P-value:9.88E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOL050C(YOL050C_d)|FD-Score:4.99|P-value:3.09E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YPR123C(YPR123C_d)|FD-Score:5.15|P-value:1.29E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPR197C(YPR197C_d)|FD-Score:3.15|P-value:8.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YRA2(YKL214C)|FD-Score:3.76|P-value:8.43E-5||SGD DESC:Member of the REF (RNA and export factor binding proteins) family; when overexpressed, can substitute for the function of Yra1p in export of poly(A)+ mRNA from the nucleus

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YJL111W4.181.45E-50.42CCT7Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance
YMR168C3.768.46E-50.21CEP3Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
YIR008C3.551.89E-40.29PRI1Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair
YJL054W3.275.42E-40.05TIM54Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane
YDR053W_d3.226.38E-40.09YDR053W_dPutative protein of unknown function; open reading frame overlaps 5' end of essential DBF4 gene encoding the regulatory subunit of the Cdc7p-Dbf4p kinase complex
YML085C3.138.71E-40.05TUB1Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication
YJL014W3.080.001030.09CCT3Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YDR180W2.990.001400.04SCC2Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; evolutionarily-conserved adherin
YGL044C2.950.001580.12RNA15Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping
YLL036C2.840.002290.08PRP19Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain
YLL003W2.760.002920.19SFI1Centrin (Cdc31p)-binding protein required for spindle pole body (SPB) duplication, localizes to the half-bridge of the SPB, required for progression through G(2)-M transition, has similarity to Xenopus laevis XCAP-C
YBR140C2.570.005070.06IRA1GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication
YKL013C2.510.005960.03ARC19Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
YHR062C2.480.006530.07RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YOL120C2.420.007850.11RPL18ARibosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR354W24.202.10E-129TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YNL084C15.101.35E-51END3EH domain-containing protein involved in endocytosis, actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p
YDR008C_d13.504.33E-42YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YOR290C12.601.56E-36SNF2Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p
YOL051W10.801.57E-27GAL11Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator
YMR216C9.412.37E-21SKY1SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1
YMR038C9.094.97E-20CCS1Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress
YDR007W8.844.82E-19TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YIL153W7.911.29E-15RRD1Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress
YML122C_d7.901.43E-15YML122C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YKL211C7.483.69E-14TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YPL254W7.445.11E-14HFI1Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions
YJR104C7.435.41E-14SOD1Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid
YMR073C7.407.07E-14IRC21Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study
YER090W7.291.54E-13TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p

GO enrichment analysis for SGTC_2741
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.3988.20E-223SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.03125
0.3938.53E-218SGTC_24885268135 45.7 μMMiscellaneous12703560.0169492
0.3903.13E-214SGTC_511013-0126 23.4 μMChemDiv (Drug-like library)16278500.0147059
0.3832.62E-205SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.0684932
0.3749.70E-196SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.03125
0.3731.57E-194SGTC_2618avocadyne 43.4 μMMicrosource (Natural product library)30151890.06
0.3713.14E-192SGTC_24785763493 32.3 μMMiscellaneous13655760.0483871
0.3672.22E-187SGTC_2669eugenol 100.0 μMMicrosource (Natural product library)33140.0566038
0.3651.76E-185SGTC_24865283202 22.4 μMMiscellaneous13777510.0175439
0.3646.24E-185SGTC_1290088-0017 176.6 μMChemDiv (Drug-like library)1824460.235294
0.3573.20E-177SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.0384615
0.3511.96E-170SGTC_18825615643 20.0 μMMiscellaneous22530750.04TRP & mitochondrial translation
0.3501.32E-169SGTC_1673st016611 27.3 μMTimTec (Natural product derivative library)52267990.0422535
0.3502.46E-169SGTC_1778st046819 58.9 μMTimTec (Natural product derivative library)6717150.0555556
0.3473.17E-166SGTC_2733amorolfine 100.0 μMMiscellaneous542600.046875plasma membrane duress

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_454lyso-paf C-166.67 μM0.675162126Miscellaneous481.6465423.08116
SGTC_4341-hexadecyl-2-methylglycero-3 PC8 μM0.6585371377ICCB bioactive library495.6731223.48906
SGTC_453enantio-paf c1610 μM0.613871343ICCB bioactive library523.6832223.4607
SGTC_455paf C-1610 μM0.6108156Miscellaneous523.6832223.4607
SGTC_435c-PAF6.7 μM0.5625124663ICCB bioactive library538.6978623.45617
SGTC_4711-acyl-paf4 μM0.53061216759367ICCB bioactive library537.6667423.22508DNA intercalators
SGTC_269exalamide29 μM0.253316Miscellaneous221.295463.05612ergosterol biosynthesis
SGTC_2248tofa1.1 μM0.244898115175Miscellaneous324.454986.85314sphingolipid biosynthesis & PDR1
SGTC_2416tofa880 nM0.244898115175Miscellaneous324.454986.85314sphingolipid biosynthesis & PDR1
SGTC_1290088-0017176.64 μM0.235294182446ChemDiv (Drug-like library)278.343483.63914
SGTC_13141222-001041.1 μM0.2352943526027ChemDiv (Drug-like library)251.321443.98514