rifampicin FDA approved compound

Inhibits bacterial DNA-dependent RNA polymerase.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2745
Screen concentration 24.3 μM
Source Miscellaneous
PubChem CID 25141428
SMILES CC1C=CC=C(C(=O)NC2=C(C3=C(C(=C4C(=C3C(=O)C2=CNN5CCN(CC5)C)C(=O)C(O4)(OC=CC(C(C(C(C(C(C1O)C)O)C)OC(=O)C)C)OC)C)C)O)O)C
Standardized SMILES COC1C=COC2(C)Oc3c(C)c(O)c4c(O)c(NC(=O)C(=CC=CC(C)C(O)C(C)C(O)C(C)C(OC(=O)C)C1C)C)c(C=NN5CCN(C)CC5)c(O)c4c3C2=O
Molecular weight 822.9402
ALogP 1.39
H-bond donor count 6
H-bond acceptor count 15
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 0
% growth inhibition (Hom. pool) 0


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 25141428
Download HIP data (tab-delimited text)  (excel)

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 25141428
Download HOP data (tab-delimited text)  (excel)
Gene:ATG23(YLR431C)|FD-Score:3.74|P-value:9.30E-5||SGD DESC:Peripheral membrane protein required for the cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p Gene:BEM3(YPL115C)|FD-Score:4.05|P-value:2.58E-5||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly Gene:GCN4(YEL009C)|FD-Score:3.15|P-value:8.23E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:HRD1(YOL013C)|FD-Score:3.22|P-value:6.32E-4||SGD DESC:Ubiquitin-protein ligase; required for endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger; likely plays a general role in targeting proteins that persistently associate with and potentially obstruct the ER-localized translocon Gene:IES4(YOR189W)|FD-Score:-3.11|P-value:9.46E-4||SGD DESC:Component of the INO80 chromatiin remodeling complex and target of the Mec1p/Tel1p DNA damage signaling pathway; proposed to link chromatin remodeling to replication checkpoint responses Gene:POP2(YNR052C)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:SFB3(YHR098C)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SOL2(YCR073W-A)|FD-Score:4.82|P-value:7.34E-7||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication Gene:TRM2(YKR056W)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:tRNA methyltransferase, 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; endo-exonuclease with a role in DNA repair Gene:YLL007C(YLL007C_p)|FD-Score:-4.02|P-value:2.86E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:ATG23(YLR431C)|FD-Score:3.74|P-value:9.30E-5||SGD DESC:Peripheral membrane protein required for the cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p Gene:BEM3(YPL115C)|FD-Score:4.05|P-value:2.58E-5||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly Gene:GCN4(YEL009C)|FD-Score:3.15|P-value:8.23E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:HRD1(YOL013C)|FD-Score:3.22|P-value:6.32E-4||SGD DESC:Ubiquitin-protein ligase; required for endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger; likely plays a general role in targeting proteins that persistently associate with and potentially obstruct the ER-localized translocon Gene:IES4(YOR189W)|FD-Score:-3.11|P-value:9.46E-4||SGD DESC:Component of the INO80 chromatiin remodeling complex and target of the Mec1p/Tel1p DNA damage signaling pathway; proposed to link chromatin remodeling to replication checkpoint responses Gene:POP2(YNR052C)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:SFB3(YHR098C)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SOL2(YCR073W-A)|FD-Score:4.82|P-value:7.34E-7||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication Gene:TRM2(YKR056W)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:tRNA methyltransferase, 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; endo-exonuclease with a role in DNA repair Gene:YLL007C(YLL007C_p)|FD-Score:-4.02|P-value:2.86E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YJL014W5.364.25E-82.76CCT3Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YGL055W2.600.004670.16OLE1Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
YKL145W2.440.007290.12RPT1One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance
YDL205C2.320.010200.10HEM3Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme
YDR023W2.220.013200.03SES1Cytosolic seryl-tRNA synthetase, class II aminoacyl-tRNA synthetase that aminoacylates tRNA(Ser), displays tRNA-dependent amino acid recognition which enhances discrimination of the serine substrate, interacts with peroxin Pex21p
YDR288W2.190.014300.02NSE3Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress
YGR198W2.170.015000.04YPP1Cargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene
YKL058W2.130.016700.08TOA2TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress
YIL078W2.050.020300.06THS1Threonyl-tRNA synthetase, essential cytoplasmic protein
YPL238C_d1.990.023300.03YPL238C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W
YJL015C_d1.960.024800.01YJL015C_dDubious open reading frame unlikely to encode a functional protein; expression if heat-inducible; located in promoter region of essential CCT3 gene encoding a subunit of the cytosolic chaperonin Cct ring complex, overlaps ORF YJL016W
YPR143W1.960.025100.01RRP15Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs
YGR119C1.950.025500.01NUP57FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p)
YLR336C1.950.025900.02SGD1Essential nuclear protein, required for biogenesis of the small ribosomal subunit; has a possible role in the osmoregulatory glycerol response; putative homolog of human NOM1 which is implicated in acute myeloid leukemia
YML031W1.930.026900.02NDC1Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YCR073W-A4.827.34E-7SOL2Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication
YPL115C4.052.58E-5BEM3Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly
YLR431C3.749.30E-5ATG23Peripheral membrane protein required for the cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p
YHR098C3.681.17E-4SFB3Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p
YKR056W3.482.47E-4TRM2tRNA methyltransferase, 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; endo-exonuclease with a role in DNA repair
YNR052C3.442.93E-4POP2RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation
YOL013C3.226.32E-4HRD1Ubiquitin-protein ligase; required for endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger; likely plays a general role in targeting proteins that persistently associate with and potentially obstruct the ER-localized translocon
YEL009C3.158.23E-4GCN4Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
YLR102C3.060.00109APC9Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
YJL112W3.030.00120MDV1Peripheral protein of cytosolic face of mitochondrial outer membrane; required for mitochondrial fission; interacts with Fis1p and with the dynamin-related GTPase Dnm1p; contains WD repeats; MDV1 has a paralog, CAF4, that arose from the whole genome duplication
YOL089C3.020.00125HAL9Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication
YGR184C2.980.00145UBR1E3 ubiquitin ligase (N-recognin); forms heterodimer with Rad6p to ubiquitinate substrates in the N-end rule pathway; regulates peptide transport via Cup9p ubiquitination; plays a major role in targeting cytosolic misfolded proteins for degradation; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS)
YJR025C2.950.00157BNA13-hydroxyanthranilic acid dioxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p
YPL040C2.930.00167ISM1Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth
YER110C2.930.00168KAP123Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1

GO enrichment analysis for SGTC_2745
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1534.30E-32SGTC_9831491-0821 12.2 μMChemDiv (Drug-like library)464951130.0393701
0.1186.94E-20SGTC_511013-0126 23.4 μMChemDiv (Drug-like library)16278500.0542636
0.1133.36E-18SGTC_3071501-1223 22.3 μMChemDiv (Drug-like library)28303290.046875
0.1111.46E-17SGTC_11710368-0077 21.4 μMChemDiv (Drug-like library)32397790.0487805
0.0953.09E-13SGTC_1912st060209 90.8 μMTimTec (Natural product derivative library)6887870.0655738
0.0936.28E-13SGTC_1697st025554 27.7 μMTimTec (Natural product derivative library)12695190.0833333
0.0921.62E-12SGTC_1875nocodazole 6.0 μMMiscellaneous41220.0601504tubulin folding & SWR complex
0.0898.04E-12SGTC_30919116372 49.5 μMChembridge (Drug-like library)171697930.072
0.0881.23E-11SGTC_2726cimetidine 79.3 μMMiscellaneous27560.0454545
0.0872.57E-11SGTC_21745790901 200.0 μMChembridge (Fragment library)7860200.0655738tubulin folding & SWR complex
0.0863.45E-11SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.0901639
0.0855.84E-11SGTC_31339127736 49.5 μMChembridge (Drug-like library)272434200.0977444
0.0849.65E-11SGTC_1654st012436 41.8 μMTimTec (Natural product derivative library)43231320.09375
0.0831.70E-10SGTC_11720370-0043 85.0 μMChemDiv (Drug-like library)3765850.0396825
0.0823.36E-10SGTC_21485546263 113.6 μMChembridge (Fragment library)7920970.0327869

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_466geldanamycin89.2 μM0.2388065288382ICCB bioactive library560.63592.3639RPP1 & pyrimidine depletion
SGTC_1578josamycin24.2 μM0.1616775282165TimTec (Pure natural product library)827.994962.957316
SGTC_2649anisomycin9.56 μM0.1578956610309ICCB bioactive library235.278980.89324
SGTC_395anisomycin6.25 μM0.1578956610309ICCB bioactive library235.278980.89324
SGTC_1872amphotericin b148 nM0.15131616667679Microsource (Natural product library)924.07902-2.6171218RNA pol III & RNase P/MRP
SGTC_2663amphotericin b100 μM0.15131616667679Microsource (Natural product library)924.07902-2.6171218
SGTC_259usnic acid108.93 μM0.148765646Miscellaneous344.315441.08427
SGTC_2690usnic acid100 μM0.148765646Miscellaneous344.315441.08427
SGTC_1079idarubicin2.36 μM0.147059636362NIH Clinical Collection533.954821.026610DNA damage response
SGTC_257daunorubicin18.5 μM0.14685330323Miscellaneous527.519860.628511anthracycline transcription coupled DNA repair
SGTC_2662daunorubicin22.37 μM0.14685330323Miscellaneous527.519860.628511anthracycline transcription coupled DNA repair
SGTC_2746daunorubicin9.88 μM0.14685330323Miscellaneous527.519860.628511anthracycline transcription coupled DNA repair
SGTC_2657nystatin74.44 μM0.14666711468707Miscellaneous926.0949-2.6041218
SGTC_277nystatin15.5 μM0.14666711468707Miscellaneous926.0949-2.6041218
SGTC_265mycophenolic acid32.93 μM0.144446541Miscellaneous320.33713.15726RPP1 & pyrimidine depletion
SGTC_2685mycophenolic acid18.85 μM0.144446541Miscellaneous320.33713.15726RNA pol III & RNase P/MRP
SGTC_920mycophenolic acid780.42 nM0.144446541TimTec (Natural product library)320.33713.15726
SGTC_2645abamectin100 μM0.1411765702208Microsource (Natural product library)873.076884.692314heme requiring