bromebric acid FDA approved compound

(E)-3-bromo-4-(4-methoxyphenyl)-4-oxobut-2-enoic acid

An antineoplastic and ion channel blocker.

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2750
Screen concentration 70.2 μM
Source Miscellaneous
PubChem CID 5358572
SMILES COC1=CC=C(C=C1)C(=O)C(=CC(=O)O)Br
Standardized SMILES COc1ccc(cc1)C(=O)C(=CC(=O)O)Br
Molecular weight 285.0908
ALogP 2.25
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 14.53
% growth inhibition (Hom. pool) 8.8


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5358572
Download HIP data (tab-delimited text)  (excel)
Gene:ALG13(YGL047W)|FD-Score:3.14|P-value:8.48E-4|Clearance:0.01||SGD DESC:Catalytic component of UDP-GlcNAc transferase; required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases; protein abundance increases in response to DNA replication stress Gene:ALG14(YBR070C)|FD-Score:3.71|P-value:1.03E-4|Clearance:0.06||SGD DESC:Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases Gene:BET5(YML077W)|FD-Score:3.94|P-value:4.00E-5|Clearance:0.04||SGD DESC:Component of the TRAPP (transport protein particle) complex, which plays an essential role in the vesicular transport from endoplasmic reticulum to Golgi Gene:CAK1(YFL029C)|FD-Score:3.91|P-value:4.67E-5|Clearance:0.14||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:CDC31(YOR257W)|FD-Score:5.34|P-value:4.66E-8|Clearance:0.72||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:EMW1(YNL313C)|FD-Score:-3.42|P-value:3.15E-4|Clearance:0||SGD DESC:Essential conserved protein with a role in cell wall integrity; contains six TPR (tetratricopeptide repeat) domains clustered in the C-terminal region; conditional mutant is suppressed by overexpression of GFA1; protein abundance increases in response to DNA replication stress Gene:MAK16(YAL025C)|FD-Score:3.76|P-value:8.38E-5|Clearance:0.05||SGD DESC:Essential nuclear protein, constituent of 66S pre-ribosomal particles; required for maturation of 25S and 5.8S rRNAs; required for maintenance of M1 satellite double-stranded RNA of the L-A virus Gene:PAM18(YLR008C)|FD-Score:-3.14|P-value:8.57E-4|Clearance:0||SGD DESC:Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); essential J-protein cochaperone that stimulates Ssc1p ATPase activity to drive import; inhibited by Pam16p Gene:PRE4(YFR050C)|FD-Score:3.65|P-value:1.32E-4|Clearance:0.23||SGD DESC:Beta 7 subunit of the 20S proteasome Gene:RPB8(YOR224C)|FD-Score:-3.64|P-value:1.36E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPL17A(YKL180W)|FD-Score:-3.25|P-value:5.73E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPP0(YLR340W)|FD-Score:4.62|P-value:1.94E-6|Clearance:0.67||SGD DESC:Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 Gene:RRP45(YDR280W)|FD-Score:3.42|P-value:3.14E-4|Clearance:0.04||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:SEC3(YER008C)|FD-Score:3.35|P-value:4.08E-4|Clearance:0.12||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress Gene:SPP2(YOR148C)|FD-Score:-3.6|P-value:1.56E-4|Clearance:0||SGD DESC:Essential protein that promotes the first step of splicing and is required for the final stages of spliceosome maturation; interacts with Prp2p, which may release Spp2p from the spliceosome following the first cleavage reaction Gene:SWI1(YPL016W)|FD-Score:3.13|P-value:8.89E-4|Clearance:0.09||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer Gene:TAF5(YBR198C)|FD-Score:-3.55|P-value:1.96E-4|Clearance:0||SGD DESC:Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:TRE2(YOR256C)|FD-Score:11.5|P-value:7.82E-31|Clearance:6.15||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication Gene:TUB1(YML085C)|FD-Score:3.22|P-value:6.48E-4|Clearance:0.08||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:YGL102C(YGL102C_d)|FD-Score:3.38|P-value:3.67E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential RPL28 gene encoding a large subunit ribosomal protein Gene:YLR339C(YLR339C_d)|FD-Score:3.23|P-value:6.17E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPP0 Gene:ALG13(YGL047W)|FD-Score:3.14|P-value:8.48E-4|Clearance:0.01||SGD DESC:Catalytic component of UDP-GlcNAc transferase; required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases; protein abundance increases in response to DNA replication stress Gene:ALG14(YBR070C)|FD-Score:3.71|P-value:1.03E-4|Clearance:0.06||SGD DESC:Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases Gene:BET5(YML077W)|FD-Score:3.94|P-value:4.00E-5|Clearance:0.04||SGD DESC:Component of the TRAPP (transport protein particle) complex, which plays an essential role in the vesicular transport from endoplasmic reticulum to Golgi Gene:CAK1(YFL029C)|FD-Score:3.91|P-value:4.67E-5|Clearance:0.14||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:CDC31(YOR257W)|FD-Score:5.34|P-value:4.66E-8|Clearance:0.72||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:EMW1(YNL313C)|FD-Score:-3.42|P-value:3.15E-4|Clearance:0||SGD DESC:Essential conserved protein with a role in cell wall integrity; contains six TPR (tetratricopeptide repeat) domains clustered in the C-terminal region; conditional mutant is suppressed by overexpression of GFA1; protein abundance increases in response to DNA replication stress Gene:MAK16(YAL025C)|FD-Score:3.76|P-value:8.38E-5|Clearance:0.05||SGD DESC:Essential nuclear protein, constituent of 66S pre-ribosomal particles; required for maturation of 25S and 5.8S rRNAs; required for maintenance of M1 satellite double-stranded RNA of the L-A virus Gene:PAM18(YLR008C)|FD-Score:-3.14|P-value:8.57E-4|Clearance:0||SGD DESC:Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); essential J-protein cochaperone that stimulates Ssc1p ATPase activity to drive import; inhibited by Pam16p Gene:PRE4(YFR050C)|FD-Score:3.65|P-value:1.32E-4|Clearance:0.23||SGD DESC:Beta 7 subunit of the 20S proteasome Gene:RPB8(YOR224C)|FD-Score:-3.64|P-value:1.36E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPL17A(YKL180W)|FD-Score:-3.25|P-value:5.73E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPP0(YLR340W)|FD-Score:4.62|P-value:1.94E-6|Clearance:0.67||SGD DESC:Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 Gene:RRP45(YDR280W)|FD-Score:3.42|P-value:3.14E-4|Clearance:0.04||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:SEC3(YER008C)|FD-Score:3.35|P-value:4.08E-4|Clearance:0.12||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress Gene:SPP2(YOR148C)|FD-Score:-3.6|P-value:1.56E-4|Clearance:0||SGD DESC:Essential protein that promotes the first step of splicing and is required for the final stages of spliceosome maturation; interacts with Prp2p, which may release Spp2p from the spliceosome following the first cleavage reaction Gene:SWI1(YPL016W)|FD-Score:3.13|P-value:8.89E-4|Clearance:0.09||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer Gene:TAF5(YBR198C)|FD-Score:-3.55|P-value:1.96E-4|Clearance:0||SGD DESC:Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:TRE2(YOR256C)|FD-Score:11.5|P-value:7.82E-31|Clearance:6.15||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication Gene:TUB1(YML085C)|FD-Score:3.22|P-value:6.48E-4|Clearance:0.08||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:YGL102C(YGL102C_d)|FD-Score:3.38|P-value:3.67E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential RPL28 gene encoding a large subunit ribosomal protein Gene:YLR339C(YLR339C_d)|FD-Score:3.23|P-value:6.17E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPP0

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5358572
Download HOP data (tab-delimited text)  (excel)
Gene:ACO1(YLR304C)|FD-Score:4.22|P-value:1.21E-5||SGD DESC:Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy Gene:AEP2(YMR282C)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:AIM41(YOR215C)|FD-Score:3.16|P-value:7.90E-4||SGD DESC:Putative protein of unknown function; the authentic protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss Gene:AIM6(YDL237W)|FD-Score:-3.5|P-value:2.35E-4||SGD DESC:Putative protein of unknown function, required for respiratory growth; YDL237W is not an essential gene Gene:APL1(YJR005W)|FD-Score:-3.58|P-value:1.74E-4||SGD DESC:Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex Gene:ARG81(YML099C)|FD-Score:-3.42|P-value:3.17E-4||SGD DESC:Zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type, involved in the regulation of arginine-responsive genes; acts with Arg80p and Arg82p Gene:ASN2(YGR124W)|FD-Score:5.58|P-value:1.23E-8||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN2 has a paralog, ASN1, that arose from the whole genome duplication Gene:ATP14(YLR295C)|FD-Score:3.31|P-value:4.67E-4||SGD DESC:Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress Gene:ATP20(YPR020W)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Subunit g of the mitochondrial F1F0 ATP synthase; reversibly phosphorylated on two residues; unphosphorylated form is required for dimerization of the ATP synthase complex Gene:BAT2(YJR148W)|FD-Score:4.03|P-value:2.83E-5||SGD DESC:Cytosolic branched-chain amino acid (BCAA) aminotransferase, preferentially involved in BCAA catabolism; homolog of murine ECA39; highly expressed during stationary phase and repressed during logarithmic phase Gene:BIT2(YBR270C_p)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Subunit of TORC2 membrane-associated complex; involved in regulation of actin cytoskeletal dynamics during polarized growth and cell wall integrity; interacts with Slm1p and Slm2p, homologous PH domain-containing TORC2 substrates; BIT2 has a paralog, BIT61, that arose from the whole genome duplication Gene:BRE4(YDL231C)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Zinc finger protein containing five transmembrane domains; null mutant exhibits strongly fragmented vacuoles and sensitivity to brefeldin A, a drug which is known to affect intracellular transport Gene:CCS1(YMR038C)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CLN1(YMR199W)|FD-Score:4.12|P-value:1.91E-5||SGD DESC:G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) Gene:CLN2(YPL256C)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) Gene:COQ5(YML110C)|FD-Score:3.83|P-value:6.51E-5||SGD DESC:2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes Gene:COT1(YOR316C)|FD-Score:5.07|P-value:2.01E-7||SGD DESC:Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistance to cobalt and rhodium; protein abundance increases in response to DNA replication stress; COT1 has a paralog, ZRC1, that arose from the whole genome duplication Gene:COX16(YJL003W)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:Mitochondrial inner membrane protein, required for assembly of cytochrome c oxidase Gene:COX20(YDR231C)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Mitochondrial inner membrane protein, required for proteolytic processing of Cox2p and its assembly into cytochrome c oxidase Gene:CUE5(YOR042W)|FD-Score:-4.02|P-value:2.93E-5||SGD DESC:Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:DIA4(YHR011W)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Probable mitochondrial seryl-tRNA synthetase, mutant displays increased invasive and pseudohyphal growth Gene:DIC1(YLR348C)|FD-Score:-4.43|P-value:4.68E-6||SGD DESC:Mitochondrial dicarboxylate carrier, integral membrane protein, catalyzes a dicarboxylate-phosphate exchange across the inner mitochondrial membrane, transports cytoplasmic dicarboxylates into the mitochondrial matrix Gene:DOG1(YHR044C)|FD-Score:-3.27|P-value:5.42E-4||SGD DESC:2-deoxyglucose-6-phosphate phosphatase; paralogous to DOG2; member of a family of low molecular weight phosphatases; confers 2-deoxyglucose resistance when overexpressed, in vivo substrate has not yet been identified Gene:DSE2(YHR143W)|FD-Score:3.38|P-value:3.60E-4||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:DTR1(YBR180W)|FD-Score:4.73|P-value:1.10E-6||SGD DESC:Putative dityrosine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for spore wall synthesis; expressed during sporulation Gene:DYN3(YMR299C)|FD-Score:4.29|P-value:9.09E-6||SGD DESC:Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration Gene:EMP65(YER140W_p)|FD-Score:-4.24|P-value:1.10E-5||SGD DESC:ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Slp1p; identified along with SLP1 in a screen for mutants defective in the unfolded protein response (UPR); proposed to function in the folding of integral membrane proteins; interacts genetically with MPS1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FDC1(YDR539W)|FD-Score:-3.51|P-value:2.21E-4||SGD DESC:Putative phenylacrylic acid decarboxylase; essential for the decarboxylation of aromatic carboxylic acids to the corresponding vinyl derivatives; homolog of E. coli UbiD; GFP-fusion protein localizes to the cytoplasm Gene:FIG4(YNL325C)|FD-Score:-3.62|P-value:1.47E-4||SGD DESC:Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain Gene:FLD1(YLR404W)|FD-Score:-5.02|P-value:2.58E-7||SGD DESC:Seipin protein involved in lipid droplet morphology, number, and size; proposed to be involved in lipid metabolism; related to the human BSCL2 which is associated with lipodystrophy Gene:FPR2(YDR519W)|FD-Score:4.63|P-value:1.86E-6||SGD DESC:Membrane-bound peptidyl-prolyl cis-trans isomerase (PPIase); binds to the drugs FK506 and rapamycin; expression pattern suggests possible involvement in ER protein trafficking; relocalizes from nucleus to vacuole upon DNA replication stress Gene:GAL3(YDR009W)|FD-Score:4.06|P-value:2.45E-5||SGD DESC:Transcriptional regulator; involved in activation of the GAL genes in response to galactose; forms a complex with Gal80p to relieve Gal80p inhibition of Gal4p; binds galactose and ATP but does not have galactokinase activity; GAL3 has a paralog, GAL1, that arose from the whole genome duplication Gene:GLC8(YMR311C)|FD-Score:3.12|P-value:8.91E-4||SGD DESC:Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress Gene:GLE2(YER107C)|FD-Score:3.23|P-value:6.27E-4||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GMC2(YLR445W)|FD-Score:-3.34|P-value:4.24E-4||SGD DESC:Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; transcription is regulated by Ume6p and induced in response to alpha factor Gene:GOT1(YMR292W)|FD-Score:-3.68|P-value:1.18E-4||SGD DESC:Homodimeric protein that is packaged into COPII vesicles and cycles between the ER and Golgi; involved in secretory transport but not directly required for aspects of transport assayed in vitro; may influence membrane composition Gene:GYP6(YJL044C)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:GTPase-activating protein (GAP) for the yeast Rab family member, Ypt6p; involved in vesicle mediated protein transport Gene:HFI1(YPL254W)|FD-Score:-3.73|P-value:9.52E-5||SGD DESC:Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions Gene:HXT8(YJL214W)|FD-Score:4.98|P-value:3.26E-7||SGD DESC:Protein of unknown function with similarity to hexose transporter family members, expression is induced by low levels of glucose and repressed by high levels of glucose Gene:INO4(YOL108C)|FD-Score:3.92|P-value:4.44E-5||SGD DESC:Transcription factor required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain Gene:LAP3(YNL239W)|FD-Score:-3.23|P-value:6.14E-4||SGD DESC:Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH Gene:LIA1(YJR070C)|FD-Score:3.83|P-value:6.37E-5||SGD DESC:Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress Gene:MEP3(YPR138C)|FD-Score:-5.1|P-value:1.74E-7||SGD DESC:Ammonium permease of high capacity and low affinity; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation ammonia permease Gene:MFB1(YDR219C)|FD-Score:-5.08|P-value:1.90E-7||SGD DESC:Mitochondria-associated F-box protein involved in maintenance of normal mitochondrial morphology; interacts with Skp1p through the F-box motif; preferentially localizes to the mother cell during budding Gene:MGR3(YMR115W)|FD-Score:-3.35|P-value:4.07E-4||SGD DESC:Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr1p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA Gene:MIC17(YMR002W)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Mitochondrial intermembrane space protein; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MNT3(YIL014W)|FD-Score:-3.82|P-value:6.75E-5||SGD DESC:Alpha-1,3-mannosyltransferase, adds the fourth and fifth alpha-1,3-linked mannose residues to O-linked glycans during protein O-glycosylation Gene:MRPL24(YMR193W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; two mitochondrial ribosomal proteins, YmL14 and YmL24, have been assigned to the same gene Gene:MRPL38(YKL170W)|FD-Score:5.15|P-value:1.32E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress Gene:MRPL8(YJL063C)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS12(YNR036C)|FD-Score:3.92|P-value:4.34E-5||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:MRPS35(YGR165W)|FD-Score:3.28|P-value:5.19E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets Gene:MUD1(YBR119W)|FD-Score:3.81|P-value:7.08E-5||SGD DESC:U1 snRNP A protein, homolog of human U1-A; involved in nuclear mRNA splicing Gene:NKP2(YLR315W)|FD-Score:6.81|P-value:4.89E-12||SGD DESC:Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein cnl2 Gene:OPI1(YHL020C)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance Gene:PEP4(YPL154C)|FD-Score:-3.64|P-value:1.34E-4||SGD DESC:Vacuolar aspartyl protease (proteinase A), required for the posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; synthesized as a zymogen, self-activates Gene:PHO91(YNR013C)|FD-Score:3.16|P-value:7.83E-4||SGD DESC:Low-affinity phosphate transporter of the vacuolar membrane; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth Gene:PIG2(YIL045W)|FD-Score:-3.36|P-value:3.90E-4||SGD DESC:Putative type-1 protein phosphatase targeting subunit that tethers Glc7p type-1 protein phosphatase to Gsy2p glycogen synthase Gene:POL32(YJR043C)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p Gene:POR2(YIL114C)|FD-Score:-4.49|P-value:3.64E-6||SGD DESC:Putative mitochondrial porin (voltage-dependent anion channel); not required for mitochondrial membrane permeability or mitochondrial osmotic stability; POR2 has a paralog, POR1, that arose from the whole genome duplication Gene:PSK2(YOL045W)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:PAS-domain containing serine/threonine protein kinase; regulates sugar flux and translation in response to an unknown metabolite by phosphorylating Ugp1p and Gsy2p (sugar flux) and Caf20p, Tif11p and Sro9p (translation); PSK2 has a paralog, PSK1, that arose from the whole genome duplication Gene:PTC4(YBR125C)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Cytoplasmic type 2C protein phosphatase (PP2C); identified as a high-copy number suppressor of cnb1 mpk1 synthetic lethality; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity Gene:RNR4(YGR180C)|FD-Score:5.72|P-value:5.17E-9||SGD DESC:Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:ROM1(YGR070W)|FD-Score:4.14|P-value:1.73E-5||SGD DESC:GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP Gene:RPE1(YJL121C)|FD-Score:4.5|P-value:3.33E-6||SGD DESC:D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress Gene:RPL13B(YMR142C)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Ribosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication Gene:RPL21B(YPL079W)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:Ribosomal 60S subunit protein L21B; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21B has a paralog, RPL21A, that arose from the whole genome duplication Gene:RPL34A(YER056C-A)|FD-Score:-3.37|P-value:3.72E-4||SGD DESC:Ribosomal 60S subunit protein L34A; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34A has a paralog, RPL34B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:5.39|P-value:3.60E-8||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS11B(YBR048W)|FD-Score:4.27|P-value:9.98E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11B has a paralog, RPS11A, that arose from the whole genome duplication Gene:RTC6(YPL183W-A)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:RVS161(YCR009C)|FD-Score:5.33|P-value:4.95E-8||SGD DESC:Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress Gene:SCW11(YGL028C)|FD-Score:3.19|P-value:7.12E-4||SGD DESC:Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on its regulation by Ste12p Gene:SEC66(YBR171W)|FD-Score:3.96|P-value:3.75E-5||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SOD1(YJR104C)|FD-Score:6.52|P-value:3.41E-11||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPC1(YJR010C-A)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Subunit of the signal peptidase complex (SPC); SPC cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER); homolog of the SPC12 subunit of mammalian signal peptidase complex; protein abundance increases in response to DNA replication stress Gene:STB2(YMR053C)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Protein that interacts with Sin3p in a two-hybrid assay and is part of a large protein complex with Sin3p and Stb1p Gene:TEF2(YBR118W)|FD-Score:3.1|P-value:9.83E-4||SGD DESC:Translational elongation factor EF-1 alpha; also encoded by TEF1; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes; may also have a role in tRNA re-export from the nucleus; TEF2 has a paralog, TEF1, that arose from the whole genome duplication Gene:TFB5(YDR079C-A)|FD-Score:3.1|P-value:9.74E-4||SGD DESC:Component of the RNA polymerase II general transcription and DNA repair factor TFIIH; involved in transcription initiation and in nucleotide-excision repair; homolog of Chlamydomonas reinhardtii REX1-S protein involved in DNA repair Gene:TPK2(YPL203W)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase Gene:TSC3(YBR058C-A)|FD-Score:5.82|P-value:2.94E-9||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TVP38(YKR088C)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; required for asymmetric localization of Kar9p during mitosis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:URM1(YIL008W)|FD-Score:3.9|P-value:4.72E-5||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:VMA13(YPR036W)|FD-Score:3.11|P-value:9.36E-4||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VPS41(YDR080W)|FD-Score:4.41|P-value:5.08E-6||SGD DESC:Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport Gene:WSS1(YHR134W)|FD-Score:9.43|P-value:2.01E-21||SGD DESC:Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response Gene:YBL055C(YBL055C)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases Gene:YBL070C(YBL070C_d)|FD-Score:3.75|P-value:8.93E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR287W(YBR287W_p)|FD-Score:3.15|P-value:8.07E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER; YBR287W is not an essential gene Gene:YCR015C(YCR015C_p)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Putative protein of unknown function; YCR015C is not an essential gene Gene:YCR087W(YCR087W_d)|FD-Score:3.1|P-value:9.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YCR087C-A; YCR087W is not an essential gene Gene:YDR115W(YDR115W_p)|FD-Score:3.76|P-value:8.55E-5||SGD DESC:Putative mitochondrial ribosomal protein of the large subunit; similar to E. coli L34 ribosomal protein; required for respiratory growth, as are most mitochondrial ribosomal proteins; protein increases in abundance and relocalizes to the plasma membrane upon DNA replication stress Gene:YEL020C(YEL020C_p)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Protein of unknown function with low sequence identity to Pdc1p; mRNA identified as translated by ribosome profiling data Gene:YFL019C(YFL019C_d)|FD-Score:6.19|P-value:2.98E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene Gene:YFL054C(YFL054C_p)|FD-Score:4.11|P-value:1.95E-5||SGD DESC:Putative channel-like protein; similar to Fps1p; mediates passive diffusion of glycerol in the presence of ethanol Gene:YGL230C(YGL230C_p)|FD-Score:-6.66|P-value:1.40E-11||SGD DESC:Putative protein of unknown function; non-essential gene Gene:YGR273C(YGR273C_p)|FD-Score:-3.4|P-value:3.40E-4||SGD DESC:Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene Gene:YHR003C(YHR003C)|FD-Score:4.41|P-value:5.14E-6||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YIM1(YMR152W)|FD-Score:-3.09|P-value:9.90E-4||SGD DESC:Protein of unknown function; null mutant displays sensitivity to DNA damaging agents; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:YIR043C(YIR043C)|FD-Score:-3.49|P-value:2.46E-4||SGD DESC:Possible pseudogene in strain S288C; YIR043C and the adjacent ORF, YIR044C, together may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family Gene:YKL068W-A(YKL068W-A_p)|FD-Score:4.27|P-value:9.78E-6||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YKU70(YMR284W)|FD-Score:-4.06|P-value:2.41E-5||SGD DESC:Subunit of the telomeric Ku complex (Yku70p-Yku80p), involved in telomere length maintenance, structure and telomere position effect; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair Gene:YLR149C(YLR149C_p)|FD-Score:4.06|P-value:2.43E-5||SGD DESC:Protein of unknown function; overexpression causes a cell cycle delay or arrest; null mutation results in a decrease in plasma membrane electron transport; YLR149C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YLR278C(YLR278C_p)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Zinc-cluster protein; GFP-fusion protein localizes to the nucleus; mutant shows moderate growth defect on caffeine; has a prion-domain like fragment that increases frequency of [URE3]; YLR278C is not an essential gene Gene:YML057C-A(YML057C-A_d)|FD-Score:3.18|P-value:7.48E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene CMP2/YML057W Gene:YNL024C(YNL024C_p)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Putative methyltransferase; has seven beta-strand methyltransferase motif; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; despite similarity to methyltransferases, null mutant does not display alterations in lysine methylation pattern Gene:YOR277C(YOR277C_d)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene CAF20 Gene:YVC1(YOR087W)|FD-Score:5.17|P-value:1.18E-7||SGD DESC:Vacuolar cation channel, mediates release of Ca(2+) from the vacuole in response to hyperosmotic shock Gene:ACO1(YLR304C)|FD-Score:4.22|P-value:1.21E-5||SGD DESC:Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy Gene:AEP2(YMR282C)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:AIM41(YOR215C)|FD-Score:3.16|P-value:7.90E-4||SGD DESC:Putative protein of unknown function; the authentic protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss Gene:AIM6(YDL237W)|FD-Score:-3.5|P-value:2.35E-4||SGD DESC:Putative protein of unknown function, required for respiratory growth; YDL237W is not an essential gene Gene:APL1(YJR005W)|FD-Score:-3.58|P-value:1.74E-4||SGD DESC:Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex Gene:ARG81(YML099C)|FD-Score:-3.42|P-value:3.17E-4||SGD DESC:Zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type, involved in the regulation of arginine-responsive genes; acts with Arg80p and Arg82p Gene:ASN2(YGR124W)|FD-Score:5.58|P-value:1.23E-8||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN2 has a paralog, ASN1, that arose from the whole genome duplication Gene:ATP14(YLR295C)|FD-Score:3.31|P-value:4.67E-4||SGD DESC:Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress Gene:ATP20(YPR020W)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Subunit g of the mitochondrial F1F0 ATP synthase; reversibly phosphorylated on two residues; unphosphorylated form is required for dimerization of the ATP synthase complex Gene:BAT2(YJR148W)|FD-Score:4.03|P-value:2.83E-5||SGD DESC:Cytosolic branched-chain amino acid (BCAA) aminotransferase, preferentially involved in BCAA catabolism; homolog of murine ECA39; highly expressed during stationary phase and repressed during logarithmic phase Gene:BIT2(YBR270C_p)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Subunit of TORC2 membrane-associated complex; involved in regulation of actin cytoskeletal dynamics during polarized growth and cell wall integrity; interacts with Slm1p and Slm2p, homologous PH domain-containing TORC2 substrates; BIT2 has a paralog, BIT61, that arose from the whole genome duplication Gene:BRE4(YDL231C)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Zinc finger protein containing five transmembrane domains; null mutant exhibits strongly fragmented vacuoles and sensitivity to brefeldin A, a drug which is known to affect intracellular transport Gene:CCS1(YMR038C)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CLN1(YMR199W)|FD-Score:4.12|P-value:1.91E-5||SGD DESC:G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) Gene:CLN2(YPL256C)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) Gene:COQ5(YML110C)|FD-Score:3.83|P-value:6.51E-5||SGD DESC:2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes Gene:COT1(YOR316C)|FD-Score:5.07|P-value:2.01E-7||SGD DESC:Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistance to cobalt and rhodium; protein abundance increases in response to DNA replication stress; COT1 has a paralog, ZRC1, that arose from the whole genome duplication Gene:COX16(YJL003W)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:Mitochondrial inner membrane protein, required for assembly of cytochrome c oxidase Gene:COX20(YDR231C)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Mitochondrial inner membrane protein, required for proteolytic processing of Cox2p and its assembly into cytochrome c oxidase Gene:CUE5(YOR042W)|FD-Score:-4.02|P-value:2.93E-5||SGD DESC:Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:DIA4(YHR011W)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Probable mitochondrial seryl-tRNA synthetase, mutant displays increased invasive and pseudohyphal growth Gene:DIC1(YLR348C)|FD-Score:-4.43|P-value:4.68E-6||SGD DESC:Mitochondrial dicarboxylate carrier, integral membrane protein, catalyzes a dicarboxylate-phosphate exchange across the inner mitochondrial membrane, transports cytoplasmic dicarboxylates into the mitochondrial matrix Gene:DOG1(YHR044C)|FD-Score:-3.27|P-value:5.42E-4||SGD DESC:2-deoxyglucose-6-phosphate phosphatase; paralogous to DOG2; member of a family of low molecular weight phosphatases; confers 2-deoxyglucose resistance when overexpressed, in vivo substrate has not yet been identified Gene:DSE2(YHR143W)|FD-Score:3.38|P-value:3.60E-4||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:DTR1(YBR180W)|FD-Score:4.73|P-value:1.10E-6||SGD DESC:Putative dityrosine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for spore wall synthesis; expressed during sporulation Gene:DYN3(YMR299C)|FD-Score:4.29|P-value:9.09E-6||SGD DESC:Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration Gene:EMP65(YER140W_p)|FD-Score:-4.24|P-value:1.10E-5||SGD DESC:ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Slp1p; identified along with SLP1 in a screen for mutants defective in the unfolded protein response (UPR); proposed to function in the folding of integral membrane proteins; interacts genetically with MPS1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FDC1(YDR539W)|FD-Score:-3.51|P-value:2.21E-4||SGD DESC:Putative phenylacrylic acid decarboxylase; essential for the decarboxylation of aromatic carboxylic acids to the corresponding vinyl derivatives; homolog of E. coli UbiD; GFP-fusion protein localizes to the cytoplasm Gene:FIG4(YNL325C)|FD-Score:-3.62|P-value:1.47E-4||SGD DESC:Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain Gene:FLD1(YLR404W)|FD-Score:-5.02|P-value:2.58E-7||SGD DESC:Seipin protein involved in lipid droplet morphology, number, and size; proposed to be involved in lipid metabolism; related to the human BSCL2 which is associated with lipodystrophy Gene:FPR2(YDR519W)|FD-Score:4.63|P-value:1.86E-6||SGD DESC:Membrane-bound peptidyl-prolyl cis-trans isomerase (PPIase); binds to the drugs FK506 and rapamycin; expression pattern suggests possible involvement in ER protein trafficking; relocalizes from nucleus to vacuole upon DNA replication stress Gene:GAL3(YDR009W)|FD-Score:4.06|P-value:2.45E-5||SGD DESC:Transcriptional regulator; involved in activation of the GAL genes in response to galactose; forms a complex with Gal80p to relieve Gal80p inhibition of Gal4p; binds galactose and ATP but does not have galactokinase activity; GAL3 has a paralog, GAL1, that arose from the whole genome duplication Gene:GLC8(YMR311C)|FD-Score:3.12|P-value:8.91E-4||SGD DESC:Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress Gene:GLE2(YER107C)|FD-Score:3.23|P-value:6.27E-4||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GMC2(YLR445W)|FD-Score:-3.34|P-value:4.24E-4||SGD DESC:Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; transcription is regulated by Ume6p and induced in response to alpha factor Gene:GOT1(YMR292W)|FD-Score:-3.68|P-value:1.18E-4||SGD DESC:Homodimeric protein that is packaged into COPII vesicles and cycles between the ER and Golgi; involved in secretory transport but not directly required for aspects of transport assayed in vitro; may influence membrane composition Gene:GYP6(YJL044C)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:GTPase-activating protein (GAP) for the yeast Rab family member, Ypt6p; involved in vesicle mediated protein transport Gene:HFI1(YPL254W)|FD-Score:-3.73|P-value:9.52E-5||SGD DESC:Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions Gene:HXT8(YJL214W)|FD-Score:4.98|P-value:3.26E-7||SGD DESC:Protein of unknown function with similarity to hexose transporter family members, expression is induced by low levels of glucose and repressed by high levels of glucose Gene:INO4(YOL108C)|FD-Score:3.92|P-value:4.44E-5||SGD DESC:Transcription factor required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain Gene:LAP3(YNL239W)|FD-Score:-3.23|P-value:6.14E-4||SGD DESC:Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH Gene:LIA1(YJR070C)|FD-Score:3.83|P-value:6.37E-5||SGD DESC:Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress Gene:MEP3(YPR138C)|FD-Score:-5.1|P-value:1.74E-7||SGD DESC:Ammonium permease of high capacity and low affinity; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation ammonia permease Gene:MFB1(YDR219C)|FD-Score:-5.08|P-value:1.90E-7||SGD DESC:Mitochondria-associated F-box protein involved in maintenance of normal mitochondrial morphology; interacts with Skp1p through the F-box motif; preferentially localizes to the mother cell during budding Gene:MGR3(YMR115W)|FD-Score:-3.35|P-value:4.07E-4||SGD DESC:Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr1p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA Gene:MIC17(YMR002W)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Mitochondrial intermembrane space protein; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MNT3(YIL014W)|FD-Score:-3.82|P-value:6.75E-5||SGD DESC:Alpha-1,3-mannosyltransferase, adds the fourth and fifth alpha-1,3-linked mannose residues to O-linked glycans during protein O-glycosylation Gene:MRPL24(YMR193W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; two mitochondrial ribosomal proteins, YmL14 and YmL24, have been assigned to the same gene Gene:MRPL38(YKL170W)|FD-Score:5.15|P-value:1.32E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress Gene:MRPL8(YJL063C)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS12(YNR036C)|FD-Score:3.92|P-value:4.34E-5||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:MRPS35(YGR165W)|FD-Score:3.28|P-value:5.19E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets Gene:MUD1(YBR119W)|FD-Score:3.81|P-value:7.08E-5||SGD DESC:U1 snRNP A protein, homolog of human U1-A; involved in nuclear mRNA splicing Gene:NKP2(YLR315W)|FD-Score:6.81|P-value:4.89E-12||SGD DESC:Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein cnl2 Gene:OPI1(YHL020C)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance Gene:PEP4(YPL154C)|FD-Score:-3.64|P-value:1.34E-4||SGD DESC:Vacuolar aspartyl protease (proteinase A), required for the posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; synthesized as a zymogen, self-activates Gene:PHO91(YNR013C)|FD-Score:3.16|P-value:7.83E-4||SGD DESC:Low-affinity phosphate transporter of the vacuolar membrane; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth Gene:PIG2(YIL045W)|FD-Score:-3.36|P-value:3.90E-4||SGD DESC:Putative type-1 protein phosphatase targeting subunit that tethers Glc7p type-1 protein phosphatase to Gsy2p glycogen synthase Gene:POL32(YJR043C)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p Gene:POR2(YIL114C)|FD-Score:-4.49|P-value:3.64E-6||SGD DESC:Putative mitochondrial porin (voltage-dependent anion channel); not required for mitochondrial membrane permeability or mitochondrial osmotic stability; POR2 has a paralog, POR1, that arose from the whole genome duplication Gene:PSK2(YOL045W)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:PAS-domain containing serine/threonine protein kinase; regulates sugar flux and translation in response to an unknown metabolite by phosphorylating Ugp1p and Gsy2p (sugar flux) and Caf20p, Tif11p and Sro9p (translation); PSK2 has a paralog, PSK1, that arose from the whole genome duplication Gene:PTC4(YBR125C)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Cytoplasmic type 2C protein phosphatase (PP2C); identified as a high-copy number suppressor of cnb1 mpk1 synthetic lethality; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity Gene:RNR4(YGR180C)|FD-Score:5.72|P-value:5.17E-9||SGD DESC:Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:ROM1(YGR070W)|FD-Score:4.14|P-value:1.73E-5||SGD DESC:GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP Gene:RPE1(YJL121C)|FD-Score:4.5|P-value:3.33E-6||SGD DESC:D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress Gene:RPL13B(YMR142C)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Ribosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication Gene:RPL21B(YPL079W)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:Ribosomal 60S subunit protein L21B; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21B has a paralog, RPL21A, that arose from the whole genome duplication Gene:RPL34A(YER056C-A)|FD-Score:-3.37|P-value:3.72E-4||SGD DESC:Ribosomal 60S subunit protein L34A; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34A has a paralog, RPL34B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:5.39|P-value:3.60E-8||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS11B(YBR048W)|FD-Score:4.27|P-value:9.98E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11B has a paralog, RPS11A, that arose from the whole genome duplication Gene:RTC6(YPL183W-A)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:RVS161(YCR009C)|FD-Score:5.33|P-value:4.95E-8||SGD DESC:Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress Gene:SCW11(YGL028C)|FD-Score:3.19|P-value:7.12E-4||SGD DESC:Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on its regulation by Ste12p Gene:SEC66(YBR171W)|FD-Score:3.96|P-value:3.75E-5||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SOD1(YJR104C)|FD-Score:6.52|P-value:3.41E-11||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPC1(YJR010C-A)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Subunit of the signal peptidase complex (SPC); SPC cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER); homolog of the SPC12 subunit of mammalian signal peptidase complex; protein abundance increases in response to DNA replication stress Gene:STB2(YMR053C)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Protein that interacts with Sin3p in a two-hybrid assay and is part of a large protein complex with Sin3p and Stb1p Gene:TEF2(YBR118W)|FD-Score:3.1|P-value:9.83E-4||SGD DESC:Translational elongation factor EF-1 alpha; also encoded by TEF1; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes; may also have a role in tRNA re-export from the nucleus; TEF2 has a paralog, TEF1, that arose from the whole genome duplication Gene:TFB5(YDR079C-A)|FD-Score:3.1|P-value:9.74E-4||SGD DESC:Component of the RNA polymerase II general transcription and DNA repair factor TFIIH; involved in transcription initiation and in nucleotide-excision repair; homolog of Chlamydomonas reinhardtii REX1-S protein involved in DNA repair Gene:TPK2(YPL203W)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase Gene:TSC3(YBR058C-A)|FD-Score:5.82|P-value:2.94E-9||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TVP38(YKR088C)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; required for asymmetric localization of Kar9p during mitosis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:URM1(YIL008W)|FD-Score:3.9|P-value:4.72E-5||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:VMA13(YPR036W)|FD-Score:3.11|P-value:9.36E-4||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VPS41(YDR080W)|FD-Score:4.41|P-value:5.08E-6||SGD DESC:Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport Gene:WSS1(YHR134W)|FD-Score:9.43|P-value:2.01E-21||SGD DESC:Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response Gene:YBL055C(YBL055C)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases Gene:YBL070C(YBL070C_d)|FD-Score:3.75|P-value:8.93E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR287W(YBR287W_p)|FD-Score:3.15|P-value:8.07E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER; YBR287W is not an essential gene Gene:YCR015C(YCR015C_p)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Putative protein of unknown function; YCR015C is not an essential gene Gene:YCR087W(YCR087W_d)|FD-Score:3.1|P-value:9.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YCR087C-A; YCR087W is not an essential gene Gene:YDR115W(YDR115W_p)|FD-Score:3.76|P-value:8.55E-5||SGD DESC:Putative mitochondrial ribosomal protein of the large subunit; similar to E. coli L34 ribosomal protein; required for respiratory growth, as are most mitochondrial ribosomal proteins; protein increases in abundance and relocalizes to the plasma membrane upon DNA replication stress Gene:YEL020C(YEL020C_p)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Protein of unknown function with low sequence identity to Pdc1p; mRNA identified as translated by ribosome profiling data Gene:YFL019C(YFL019C_d)|FD-Score:6.19|P-value:2.98E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene Gene:YFL054C(YFL054C_p)|FD-Score:4.11|P-value:1.95E-5||SGD DESC:Putative channel-like protein; similar to Fps1p; mediates passive diffusion of glycerol in the presence of ethanol Gene:YGL230C(YGL230C_p)|FD-Score:-6.66|P-value:1.40E-11||SGD DESC:Putative protein of unknown function; non-essential gene Gene:YGR273C(YGR273C_p)|FD-Score:-3.4|P-value:3.40E-4||SGD DESC:Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene Gene:YHR003C(YHR003C)|FD-Score:4.41|P-value:5.14E-6||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YIM1(YMR152W)|FD-Score:-3.09|P-value:9.90E-4||SGD DESC:Protein of unknown function; null mutant displays sensitivity to DNA damaging agents; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:YIR043C(YIR043C)|FD-Score:-3.49|P-value:2.46E-4||SGD DESC:Possible pseudogene in strain S288C; YIR043C and the adjacent ORF, YIR044C, together may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family Gene:YKL068W-A(YKL068W-A_p)|FD-Score:4.27|P-value:9.78E-6||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YKU70(YMR284W)|FD-Score:-4.06|P-value:2.41E-5||SGD DESC:Subunit of the telomeric Ku complex (Yku70p-Yku80p), involved in telomere length maintenance, structure and telomere position effect; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair Gene:YLR149C(YLR149C_p)|FD-Score:4.06|P-value:2.43E-5||SGD DESC:Protein of unknown function; overexpression causes a cell cycle delay or arrest; null mutation results in a decrease in plasma membrane electron transport; YLR149C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YLR278C(YLR278C_p)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Zinc-cluster protein; GFP-fusion protein localizes to the nucleus; mutant shows moderate growth defect on caffeine; has a prion-domain like fragment that increases frequency of [URE3]; YLR278C is not an essential gene Gene:YML057C-A(YML057C-A_d)|FD-Score:3.18|P-value:7.48E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene CMP2/YML057W Gene:YNL024C(YNL024C_p)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Putative methyltransferase; has seven beta-strand methyltransferase motif; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; despite similarity to methyltransferases, null mutant does not display alterations in lysine methylation pattern Gene:YOR277C(YOR277C_d)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene CAF20 Gene:YVC1(YOR087W)|FD-Score:5.17|P-value:1.18E-7||SGD DESC:Vacuolar cation channel, mediates release of Ca(2+) from the vacuole in response to hyperosmotic shock

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR256C11.507.82E-316.15TRE2Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication
YOR257W5.344.66E-80.72CDC31Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation
YLR340W4.621.94E-60.67RPP0Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10
YML077W3.944.00E-50.04BET5Component of the TRAPP (transport protein particle) complex, which plays an essential role in the vesicular transport from endoplasmic reticulum to Golgi
YFL029C3.914.67E-50.14CAK1Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases
YAL025C3.768.38E-50.05MAK16Essential nuclear protein, constituent of 66S pre-ribosomal particles; required for maturation of 25S and 5.8S rRNAs; required for maintenance of M1 satellite double-stranded RNA of the L-A virus
YBR070C3.711.03E-40.06ALG14Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases
YFR050C3.651.32E-40.23PRE4Beta 7 subunit of the 20S proteasome
YDR280W3.423.14E-40.04RRP45Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress
YGL102C_d3.383.67E-40.03YGL102C_dDubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential RPL28 gene encoding a large subunit ribosomal protein
YER008C3.354.08E-40.12SEC3Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress
YLR339C_d3.236.17E-40.01YLR339C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPP0
YML085C3.226.48E-40.08TUB1Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication
YGL047W3.148.48E-40.01ALG13Catalytic component of UDP-GlcNAc transferase; required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases; protein abundance increases in response to DNA replication stress
YPL016W3.138.89E-40.09SWI1Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YHR134W9.432.01E-21WSS1Sumoylated protein that localizes to a single spot on the nuclear periphery of mother cells but not daughters; interacts genetically with SMT3; UV-sensitive mutant phenotype and genetic interactions suggest a role in the DNA damage response
YLR315W6.814.89E-12NKP2Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein cnl2
YJR104C6.523.41E-11SOD1Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid
YFL019C_d6.192.98E-10YFL019C_dDubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene
YBR058C-A5.822.94E-9TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YGR180C5.725.17E-9RNR4Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress
YGR124W5.581.23E-8ASN2Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN2 has a paralog, ASN1, that arose from the whole genome duplication
YDL020C5.393.60E-8RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YCR009C5.334.95E-8RVS161Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress
YOR087W5.171.18E-7YVC1Vacuolar cation channel, mediates release of Ca(2+) from the vacuole in response to hyperosmotic shock
YKL170W5.151.32E-7MRPL38Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress
YOR316C5.072.01E-7COT1Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistance to cobalt and rhodium; protein abundance increases in response to DNA replication stress; COT1 has a paralog, ZRC1, that arose from the whole genome duplication
YJL214W4.983.26E-7HXT8Protein of unknown function with similarity to hexose transporter family members, expression is induced by low levels of glucose and repressed by high levels of glucose
YBR180W4.731.10E-6DTR1Putative dityrosine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for spore wall synthesis; expressed during sporulation
YDR519W4.631.86E-6FPR2Membrane-bound peptidyl-prolyl cis-trans isomerase (PPIase); binds to the drugs FK506 and rapamycin; expression pattern suggests possible involvement in ER protein trafficking; relocalizes from nucleus to vacuole upon DNA replication stress

GO enrichment analysis for SGTC_2750
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1611.40E-35SGTC_720r052-1644 49.9 μMChemDiv (Drug-like library)14228630.111111TSC3-RPN4
0.1476.25E-30SGTC_28pentachloropyridine N-oxide 1.6 μMChemDiv (Drug-like library)1402500.0238095TSC3-RPN4
0.1461.97E-29SGTC_2526patulin 37.6 μMMicrosource (Natural product library)46960.0754717
0.1331.03E-24SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.114286TSC3-RPN4
0.1322.99E-24SGTC_2701st077767 14.7 μMTimTec (Natural product derivative library)57211880.140351
0.1163.12E-19SGTC_26602',4'-dihydroxychalcone 17.1 μMTimTec (Pure natural product library)53572180.156863
0.1163.25E-19SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.125TSC3-RPN4
0.1165.35E-19SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0338983TSC3-RPN4
0.1132.44E-18SGTC_2499avocadenofuran 51.5 μMMicrosource (Natural product library)68577920.0754717
0.1134.00E-18SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.157895TSC3-RPN4
0.1063.33E-16SGTC_1813st051246 8.6 μMTimTec (Natural product derivative library)52919590.173077TSC3-RPN4
0.1041.31E-15SGTC_4283448-4153 16.5 μMChemDiv (Drug-like library)38348740.135135TSC3-RPN4
0.1032.27E-15SGTC_22256654152 15.4 μMChembridge (Fragment library)975820.106383
0.1023.41E-15SGTC_328k915-0120 63.5 μMChemDiv (Drug-like library)66245430.190476TSC3-RPN4
0.1001.63E-14SGTC_20905301565 104.4 μMChembridge (Fragment library)20563440.0689655TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_19284'-methoxychalcone40.3 μM0.395349641818TimTec (Natural product derivative library)238.281163.68502
SGTC_24984'-Methoxychalcone54.63 μM0.395349641818Microsource (Natural product library)238.281163.68502RPP1 & pyrimidine depletion
SGTC_70192-15824.23 μM0.377778252616ChemDiv (Drug-like library)398.089164.78702
SGTC_9383126-1755342 μM0.3751777771ChemDiv (Drug-like library)390.388723.01235
SGTC_2481547894845.16 μM0.36956577792Miscellaneous270.279962.89614PDR1
SGTC_5371334-0398209 μM0.3333335846986ChemDiv (Drug-like library)270.283263.06225
SGTC_11730443-0020122 μM0.3333335331295ChemDiv (Drug-like library)254.280563.44313TSC3-RPN4
SGTC_2491paeonol100 μM0.33333311092Microsource (Natural product library)166.17391.31113
SGTC_2961222-003974.9 μM0.3260875984176ChemDiv (Drug-like library)218.291524.22812
SGTC_25772',4'-dihydroxy-4-methoxychalcone15.32 μM0.325711223Microsource (Natural product library)270.279963.20124