dehydroepiandrosterone FDA approved compound

(3S,8R,9S,10R,13S,14S)-3-hydroxy-10,13-dimethyl-1,2,3,4,7,8,9,11,12,14,15,16-dodecahydrocyclopenta[a]phenanthren-17-one

A major C19 steroid produced by the adrenal cortex. It is also produced in small quantities in the testis and the ovary. Dehydroepiandrosterone (DHEA) can be converted to testosterone; androstenedione; estradiol; and estrone. Most of DHEA is sulfated (dehydroepiandrosterone sulfate) before secretion. [PubChem]

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2758
Screen concentration 69.3 μM
Source Miscellaneous
PubChem CID 5881
SMILES CC12CCC3C(C1CCC2=O)CC=C4C3(CCC(C4)O)C
Standardized SMILES CC12CCC3C(CC=C4CC(O)CCC34C)C1CCC2=O
Molecular weight 288.4244
ALogP 3.34
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 18.98
% growth inhibition (Hom. pool) 9.47


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5881
Download HIP data (tab-delimited text)  (excel)
Gene:BET5(YML077W)|FD-Score:5.21|P-value:9.39E-8|Clearance:0.96||SGD DESC:Component of the TRAPP (transport protein particle) complex, which plays an essential role in the vesicular transport from endoplasmic reticulum to Golgi Gene:CBK1(YNL161W)|FD-Score:3.46|P-value:2.66E-4|Clearance:0.03||SGD DESC:Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress Gene:CDC24(YAL041W)|FD-Score:3.43|P-value:3.02E-4|Clearance:0.06||SGD DESC:Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:CTF13(YMR094W)|FD-Score:3.5|P-value:2.36E-4|Clearance:0.03||SGD DESC:Subunit of the CBF3 complex, which binds to the CDE III element of centromeres, bending the DNA upon binding, and may be involved in sister chromatid cohesion during mitosis Gene:DED81(YHR019C)|FD-Score:3.24|P-value:6.06E-4|Clearance:0.05||SGD DESC:Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA Gene:GCD11(YER025W)|FD-Score:-4.15|P-value:1.66E-5|Clearance:0||SGD DESC:Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met Gene:HAS1(YMR290C)|FD-Score:3.18|P-value:7.29E-4|Clearance:0.01||SGD DESC:ATP-dependent RNA helicase; localizes to both the nuclear periphery and nucleolus; highly enriched in nuclear pore complex fractions; constituent of 66S pre-ribosomal particles Gene:KRE9(YJL174W)|FD-Score:4.15|P-value:1.68E-5|Clearance:0.09||SGD DESC:Glycoprotein involved in cell wall beta-glucan assembly; null mutation leads to severe growth defects, aberrant multibudded morphology, and mating defects Gene:MCM10(YIL150C)|FD-Score:-3.64|P-value:1.35E-4|Clearance:0||SGD DESC:Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins Gene:MMF1(YIL051C)|FD-Score:-3.76|P-value:8.61E-5|Clearance:0||SGD DESC:Mitochondrial protein required for transamination of isoleucine but not of valine or leucine; may regulate specificity of branched-chain transaminases Bat1p and Bat2p; interacts genetically with mitochondrial ribosomal protein genes Gene:MNP1(YGL068W)|FD-Score:3.37|P-value:3.82E-4|Clearance:0.02||SGD DESC:Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth Gene:NOB1(YOR056C)|FD-Score:3.16|P-value:8.02E-4|Clearance:0.04||SGD DESC:Protein involved in proteasomal and 40S ribosomal subunit biogenesis; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA; cleavage is activated by Fun12p, a GTPase and translation initiation factor; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:OLE1(YGL055W)|FD-Score:3.88|P-value:5.14E-5|Clearance:0.13||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:POP3(YNL282W)|FD-Score:5.81|P-value:3.14E-9|Clearance:0.96||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRP19(YLL036C)|FD-Score:3.27|P-value:5.35E-4|Clearance:0.04||SGD DESC:Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain Gene:RFA2(YNL312W)|FD-Score:-3.99|P-value:3.35E-5|Clearance:0||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress Gene:RFC3(YNL290W)|FD-Score:3.11|P-value:9.24E-4|Clearance:0.02||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RNR2(YJL026W)|FD-Score:4.26|P-value:1.04E-5|Clearance:0.11||SGD DESC:Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits Gene:RPC31(YNL151C)|FD-Score:-3.34|P-value:4.26E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C31 Gene:RRP17(YDR412W)|FD-Score:3.75|P-value:8.87E-5|Clearance:0.25||SGD DESC:Component of the pre-60S pre-ribosomal particle; required for cell viability under standard (aerobic) conditions but not under anaerobic conditions; exonuclease required for 5′ end processing of pre-60S ribosomal RNA Gene:SEN54(YPL083C)|FD-Score:3.35|P-value:4.08E-4|Clearance:0.01||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p Gene:TAF12(YDR145W)|FD-Score:3.1|P-value:9.74E-4|Clearance:0.21||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:TIM23(YNR017W)|FD-Score:3.17|P-value:7.55E-4|Clearance:0.02||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein import into mitochondrial matrix and inner membrane; with Tim17p, contributes to architecture and function of the import channel Gene:TRS20(YBR254C)|FD-Score:3.33|P-value:4.30E-4|Clearance:0.06||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; mutations in the human homolog cause the spondyloepiphyseal dysplasia tarda (SEDL) disorder Gene:TSC10(YBR265W)|FD-Score:3.97|P-value:3.57E-5|Clearance:0.09||SGD DESC:3-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family Gene:UTP4(YDR324C)|FD-Score:4.06|P-value:2.48E-5|Clearance:0.09||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript Gene:YAH1(YPL252C)|FD-Score:3.47|P-value:2.64E-4|Clearance:0||SGD DESC:Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin Gene:YCL041C(YCL041C_d)|FD-Score:6.71|P-value:9.48E-12|Clearance:0.96||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps both the verified gene PDI1/YCL043C and the uncharacterized gene YCL042W Gene:BET5(YML077W)|FD-Score:5.21|P-value:9.39E-8|Clearance:0.96||SGD DESC:Component of the TRAPP (transport protein particle) complex, which plays an essential role in the vesicular transport from endoplasmic reticulum to Golgi Gene:CBK1(YNL161W)|FD-Score:3.46|P-value:2.66E-4|Clearance:0.03||SGD DESC:Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress Gene:CDC24(YAL041W)|FD-Score:3.43|P-value:3.02E-4|Clearance:0.06||SGD DESC:Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:CTF13(YMR094W)|FD-Score:3.5|P-value:2.36E-4|Clearance:0.03||SGD DESC:Subunit of the CBF3 complex, which binds to the CDE III element of centromeres, bending the DNA upon binding, and may be involved in sister chromatid cohesion during mitosis Gene:DED81(YHR019C)|FD-Score:3.24|P-value:6.06E-4|Clearance:0.05||SGD DESC:Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA Gene:GCD11(YER025W)|FD-Score:-4.15|P-value:1.66E-5|Clearance:0||SGD DESC:Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met Gene:HAS1(YMR290C)|FD-Score:3.18|P-value:7.29E-4|Clearance:0.01||SGD DESC:ATP-dependent RNA helicase; localizes to both the nuclear periphery and nucleolus; highly enriched in nuclear pore complex fractions; constituent of 66S pre-ribosomal particles Gene:KRE9(YJL174W)|FD-Score:4.15|P-value:1.68E-5|Clearance:0.09||SGD DESC:Glycoprotein involved in cell wall beta-glucan assembly; null mutation leads to severe growth defects, aberrant multibudded morphology, and mating defects Gene:MCM10(YIL150C)|FD-Score:-3.64|P-value:1.35E-4|Clearance:0||SGD DESC:Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins Gene:MMF1(YIL051C)|FD-Score:-3.76|P-value:8.61E-5|Clearance:0||SGD DESC:Mitochondrial protein required for transamination of isoleucine but not of valine or leucine; may regulate specificity of branched-chain transaminases Bat1p and Bat2p; interacts genetically with mitochondrial ribosomal protein genes Gene:MNP1(YGL068W)|FD-Score:3.37|P-value:3.82E-4|Clearance:0.02||SGD DESC:Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth Gene:NOB1(YOR056C)|FD-Score:3.16|P-value:8.02E-4|Clearance:0.04||SGD DESC:Protein involved in proteasomal and 40S ribosomal subunit biogenesis; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA; cleavage is activated by Fun12p, a GTPase and translation initiation factor; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:OLE1(YGL055W)|FD-Score:3.88|P-value:5.14E-5|Clearance:0.13||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:POP3(YNL282W)|FD-Score:5.81|P-value:3.14E-9|Clearance:0.96||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRP19(YLL036C)|FD-Score:3.27|P-value:5.35E-4|Clearance:0.04||SGD DESC:Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain Gene:RFA2(YNL312W)|FD-Score:-3.99|P-value:3.35E-5|Clearance:0||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress Gene:RFC3(YNL290W)|FD-Score:3.11|P-value:9.24E-4|Clearance:0.02||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RNR2(YJL026W)|FD-Score:4.26|P-value:1.04E-5|Clearance:0.11||SGD DESC:Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits Gene:RPC31(YNL151C)|FD-Score:-3.34|P-value:4.26E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C31 Gene:RRP17(YDR412W)|FD-Score:3.75|P-value:8.87E-5|Clearance:0.25||SGD DESC:Component of the pre-60S pre-ribosomal particle; required for cell viability under standard (aerobic) conditions but not under anaerobic conditions; exonuclease required for 5′ end processing of pre-60S ribosomal RNA Gene:SEN54(YPL083C)|FD-Score:3.35|P-value:4.08E-4|Clearance:0.01||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p Gene:TAF12(YDR145W)|FD-Score:3.1|P-value:9.74E-4|Clearance:0.21||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:TIM23(YNR017W)|FD-Score:3.17|P-value:7.55E-4|Clearance:0.02||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein import into mitochondrial matrix and inner membrane; with Tim17p, contributes to architecture and function of the import channel Gene:TRS20(YBR254C)|FD-Score:3.33|P-value:4.30E-4|Clearance:0.06||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; mutations in the human homolog cause the spondyloepiphyseal dysplasia tarda (SEDL) disorder Gene:TSC10(YBR265W)|FD-Score:3.97|P-value:3.57E-5|Clearance:0.09||SGD DESC:3-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family Gene:UTP4(YDR324C)|FD-Score:4.06|P-value:2.48E-5|Clearance:0.09||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript Gene:YAH1(YPL252C)|FD-Score:3.47|P-value:2.64E-4|Clearance:0||SGD DESC:Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin Gene:YCL041C(YCL041C_d)|FD-Score:6.71|P-value:9.48E-12|Clearance:0.96||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps both the verified gene PDI1/YCL043C and the uncharacterized gene YCL042W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5881
Download HOP data (tab-delimited text)  (excel)
Gene:AAC1(YMR056C)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; phosphorylated; Aac1p is a minor isoform while Pet9p is the major ADP/ATP translocator; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:AGE1(YDR524C)|FD-Score:3.79|P-value:7.61E-5||SGD DESC:ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in the secretory and endocytic pathways; contains C2C2H2 cysteine/histidine motif Gene:ANT1(YPR128C)|FD-Score:3.19|P-value:7.03E-4||SGD DESC:Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation Gene:ARG3(YJL088W)|FD-Score:3.11|P-value:9.28E-4||SGD DESC:Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine Gene:ARG5,6(YER069W)|FD-Score:7.38|P-value:7.96E-14||SGD DESC:Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine Gene:ARO1(YDR127W)|FD-Score:16.1|P-value:1.33E-58||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO2(YGL148W)|FD-Score:6.35|P-value:1.06E-10||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ARO3(YDR035W)|FD-Score:14.3|P-value:5.81E-47||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ASH1(YKL185W)|FD-Score:-3.11|P-value:9.23E-4||SGD DESC:Zinc-finger inhibitor of HO transcription; mRNA is localized and translated in the distal tip of anaphase cells, resulting in accumulation of Ash1p in daughter cell nuclei and inhibition of HO expression; potential Cdc28p substrate Gene:ASR1(YPR093C)|FD-Score:4.35|P-value:6.73E-6||SGD DESC:Ubiquitin ligase that modifies and regulates RNA Pol II; involved in a putative alcohol-responsive signaling pathway; accumulates in the nucleus under alcohol stress; contains a Ring/PHD finger domain similar to the mammalian rA9 protein Gene:BIT2(YBR270C_p)|FD-Score:4.15|P-value:1.63E-5||SGD DESC:Subunit of TORC2 membrane-associated complex; involved in regulation of actin cytoskeletal dynamics during polarized growth and cell wall integrity; interacts with Slm1p and Slm2p, homologous PH domain-containing TORC2 substrates; BIT2 has a paralog, BIT61, that arose from the whole genome duplication Gene:BUD21(YOR078W)|FD-Score:-4.59|P-value:2.21E-6||SGD DESC:Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated as bud-site selection mutant that displays a random budding pattern Gene:CAR1(YPL111W)|FD-Score:7.71|P-value:6.53E-15||SGD DESC:Arginase, responsible for arginine degradation, expression responds to both induction by arginine and nitrogen catabolite repression; disruption enhances freeze tolerance Gene:CIR2(YOR356W)|FD-Score:7.82|P-value:2.65E-15||SGD DESC:Putative ortholog of human electron transfer flavoprotein dehydrogenase (ETF-dH); found in a large supramolecular complex with other mitochondrial dehydrogenases; may have a role in oxidative stress response Gene:COG7(YGL005C)|FD-Score:3.2|P-value:6.88E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:4.32|P-value:7.90E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CRN1(YLR429W)|FD-Score:4.93|P-value:4.20E-7||SGD DESC:Coronin, cortical actin cytoskeletal component that associates with the Arp2p/Arp3p complex to regulate its activity; plays a role in regulation of actin patch assembly Gene:CTH1(YDR151C)|FD-Score:-3.57|P-value:1.82E-4||SGD DESC:Member of the CCCH zinc finger family; has similarity to mammalian Tis11 protein, which activates transcription and also has a role in mRNA degradation; may function with Tis11p in iron homeostasis Gene:DIC1(YLR348C)|FD-Score:-4.3|P-value:8.39E-6||SGD DESC:Mitochondrial dicarboxylate carrier, integral membrane protein, catalyzes a dicarboxylate-phosphate exchange across the inner mitochondrial membrane, transports cytoplasmic dicarboxylates into the mitochondrial matrix Gene:DUF1(YOL087C)|FD-Score:3.2|P-value:6.93E-4||SGD DESC:Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid Gene:ECM33(YBR078W)|FD-Score:-4.25|P-value:1.08E-5||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:FAR3(YMR052W)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:FEN2(YCR028C)|FD-Score:-9.06|P-value:6.25E-20||SGD DESC:Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:GCN2(YDR283C)|FD-Score:8.33|P-value:3.91E-17||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN20(YFR009W)|FD-Score:5.16|P-value:1.25E-7||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:12.4|P-value:1.23E-35||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:25.3|P-value:1.67E-141||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GGA1(YDR358W)|FD-Score:3.84|P-value:6.18E-5||SGD DESC:Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi Gene:GIP1(YBR045C)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:Meiosis-specific regulatory subunit of the Glc7p protein phosphatase, regulates spore wall formation and septin organization, required for expression of some late meiotic genes and for normal localization of Glc7p Gene:GPD1(YDL022W)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity Gene:GPD2(YOL059W)|FD-Score:7.54|P-value:2.42E-14||SGD DESC:NAD-dependent glycerol 3-phosphate dehydrogenase, homolog of Gpd1p, expression is controlled by an oxygen-independent signaling pathway required to regulate metabolism under anoxic conditions; located in cytosol and mitochondria; constitutively active but is inactivated via phosphorylation by energy-stress responsive kinase SNF1 Gene:GPT2(YKR067W)|FD-Score:4.51|P-value:3.25E-6||SGD DESC:Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen Gene:GSF2(YML048W)|FD-Score:4.71|P-value:1.27E-6||SGD DESC:ER localized integral membrane protein that may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression Gene:HNT3(YOR258W)|FD-Score:-3.29|P-value:5.01E-4||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:HOR7(YMR251W-A)|FD-Score:-3.21|P-value:6.60E-4||SGD DESC:Protein of unknown function; overexpression suppresses Ca2+ sensitivity of mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p; transcription is induced under hyperosmotic stress and repressed by alpha factor; HOR7 has a paralog, DDR2, that arose from the whole genome duplication Gene:HTD2(YHR067W)|FD-Score:-4.94|P-value:3.84E-7||SGD DESC:Mitochondrial 3-hydroxyacyl-thioester dehydratase involved in fatty acid biosynthesis, required for respiratory growth and for normal mitochondrial morphology Gene:IDP2(YLR174W)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:Cytosolic NADP-specific isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate; levels are elevated during growth on non-fermentable carbon sources and reduced during growth on glucose; IDP2 has a paralog, IDP3, that arose from the whole genome duplication Gene:INP51(YIL002C)|FD-Score:3.34|P-value:4.25E-4||SGD DESC:Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth Gene:IRC16(YPR038W_d)|FD-Score:3.17|P-value:7.54E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene YPR037C; null mutant displays increased levels of spontaneous Rad52p foci Gene:ISU1(YPL135W)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Conserved protein of the mitochondrial matrix, performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable Gene:KAP123(YER110C)|FD-Score:-3.27|P-value:5.42E-4||SGD DESC:Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1 Gene:KIN2(YLR096W)|FD-Score:3.12|P-value:9.17E-4||SGD DESC:Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p Gene:LRO1(YNR008W)|FD-Score:4.02|P-value:2.93E-5||SGD DESC:Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase Gene:LSB5(YCL034W)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Protein of unknown function; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; may mediate disassembly of the Pan1 complex from the endocytic coat Gene:MDM34(YGL219C)|FD-Score:3.37|P-value:3.72E-4||SGD DESC:Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MET17(YLR303W)|FD-Score:6.39|P-value:8.13E-11||SGD DESC:Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis Gene:MET3(YJR010W)|FD-Score:-3.22|P-value:6.32E-4||SGD DESC:ATP sulfurylase, catalyzes the primary step of intracellular sulfate activation, essential for assimilatory reduction of sulfate to sulfide, involved in methionine metabolism Gene:MF(ALPHA)2(YGL089C)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:Mating pheromone alpha-factor, made by alpha cells; interacts with mating type a cells to induce cell cycle arrest and other responses leading to mating; also encoded by MF(ALPHA)1, which is more highly expressed than MF(ALPHA)2 Gene:MMS2(YGL087C)|FD-Score:-3.13|P-value:8.84E-4||SGD DESC:Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress Gene:MNR2(YKL064W)|FD-Score:4.48|P-value:3.73E-6||SGD DESC:Vacuolar membrane protein required for magnesium homeostasis; putative magnesium transporter; has similarity to Alr1p and Alr2p, which mediate influx of Mg2+ and other divalent cations Gene:MSO1(YNR049C)|FD-Score:3.31|P-value:4.68E-4||SGD DESC:Probable component of the secretory vesicle docking complex; acts at a late step in secretion; shows genetic and physical interactions with Sec1p; required for prospore membrane formation during sporulation; relocalizes from bud neck to nucleus upon DNA replication stress Gene:MSY1(YPL097W)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Mitochondrial tyrosyl-tRNA synthetase Gene:NIT3(YLR351C)|FD-Score:3.81|P-value:6.85E-5||SGD DESC:Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member Gene:NMA111(YNL123W)|FD-Score:4.49|P-value:3.64E-6||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:NOP6(YDL213C)|FD-Score:-3.16|P-value:7.91E-4||SGD DESC:rRNA-binding protein required for 40S ribosomal subunit biogenesis; contains an RNA recognition motif (RRM); hydrophilin essential to overcome the stress of the desiccation-rehydration process; NOP6 may be a fungal-specific gene as no homologs have been yet identified in higher eukaryotes Gene:NUP170(YBL079W)|FD-Score:-3.92|P-value:4.52E-5||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication Gene:OPI3(YJR073C)|FD-Score:3.89|P-value:5.08E-5||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:ORT1(YOR130C)|FD-Score:3.95|P-value:3.84E-5||SGD DESC:Ornithine transporter of the mitochondrial inner membrane, exports ornithine from mitochondria as part of arginine biosynthesis; human ortholog is associated with hyperammonaemia-hyperornithinaemia-homocitrullinuria (HHH) syndrome Gene:PDR1(YGL013C)|FD-Score:-3.32|P-value:4.54E-4||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PDR11(YIL013C)|FD-Score:3.11|P-value:9.33E-4||SGD DESC:ATP-binding cassette (ABC) transporter, multidrug transporter involved in multiple drug resistance; mediates sterol uptake when sterol biosynthesis is compromised; regulated by Pdr1p; required for anaerobic growth Gene:PDR16(YNL231C)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress Gene:PEX1(YKL197C)|FD-Score:-3.84|P-value:6.05E-5||SGD DESC:AAA-peroxin that heterodimerizes with AAA-peroxin Pex6p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol; induced by oleic acid and upregulated during anaerobiosis Gene:PKH2(YOL100W)|FD-Score:-3.62|P-value:1.46E-4||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p Gene:PKP2(YGL059W)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Mitochondrial protein kinase; negatively regulates activity of the pyruvate dehydrogenase complex by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp1p and phosphatases Ptc5p and Ptc6p; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:POP2(YNR052C)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PRM8(YGL053W)|FD-Score:4.42|P-value:4.92E-6||SGD DESC:Pheromone-regulated protein with 2 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; forms a complex with Prp9p in the ER; member of DUP240 gene family Gene:ROM2(YLR371W)|FD-Score:-6.14|P-value:4.19E-10||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL22B(YFL034C-A)|FD-Score:4|P-value:3.14E-5||SGD DESC:Ribosomal 60S subunit protein L22B; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22B has a paralog, RPL22A, that arose from the whole genome duplication Gene:RRG7(YOR305W)|FD-Score:-3.24|P-value:5.88E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YOR305W is not an essential gene Gene:RTC6(YPL183W-A)|FD-Score:-3.26|P-value:5.65E-4||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:SAC6(YDR129C)|FD-Score:-3.12|P-value:9.13E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SAN1(YDR143C)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Ubiquitin-protein ligase; involved in the proteasome-dependent degradation of aberrant nuclear proteins; targets substrates with regions of exposed hydrophobicity containing 5 or more contiguous hydrophobic residues; contains intrinsically disordered regions that contribute to substrate recognition Gene:SLG1(YOR008C)|FD-Score:-3.29|P-value:5.09E-4||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SNF12(YNR023W)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive Gene:SPG3(YDR504C)|FD-Score:-3.5|P-value:2.34E-4||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources Gene:SRT1(YMR101C)|FD-Score:4.11|P-value:2.00E-5||SGD DESC:Cis-prenyltransferase involved in synthesis of long-chain dolichols (19-22 isoprene units; as opposed to Rer2p which synthesizes shorter-chain dolichols); localizes to lipid bodies; transcription is induced during stationary phase Gene:STB6(YKL072W)|FD-Score:-3.55|P-value:1.91E-4||SGD DESC:Protein that binds Sin3p in a two-hybrid assay Gene:SVF1(YDR346C)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Protein with a potential role in cell survival pathways, required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance Gene:SVP26(YHR181W)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Integral membrane protein of the early Golgi apparatus and endoplasmic reticulum, involved in COP II vesicle transport; may also function to promote retention of proteins in the early Golgi compartment Gene:SWC7(YLR385C)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:Protein of unknown function, component of the Swr1p complex that incorporates Htz1p into chromatin Gene:TAT1(YBR069C)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TDA11(YHR159W_p)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele Gene:TMS1(YDR105C)|FD-Score:4.73|P-value:1.15E-6||SGD DESC:Vacuolar membrane protein of unknown function that is conserved in mammals; predicted to contain eleven transmembrane helices; interacts with Pdr5p, a protein involved in multidrug resistance Gene:TRE1(YPL176C)|FD-Score:4.3|P-value:8.72E-6||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:TRP1(YDR007W)|FD-Score:21.8|P-value:3.32E-105||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:22.4|P-value:1.17E-111||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:27.1|P-value:1.29E-161||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:24.7|P-value:2.50E-135||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:10.5|P-value:4.98E-26||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TSR2(YLR435W)|FD-Score:-3.29|P-value:5.09E-4||SGD DESC:Protein with a potential role in pre-rRNA processing Gene:UBP3(YER151C)|FD-Score:-3.25|P-value:5.70E-4||SGD DESC:Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress Gene:UBP5(YER144C)|FD-Score:3.12|P-value:8.97E-4||SGD DESC:Putative ubiquitin-specific protease, closest paralog of Doa4p but has no functional overlap; concentrates at the bud neck Gene:UBP6(YFR010W)|FD-Score:-3.46|P-value:2.72E-4||SGD DESC:Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance Gene:UGX2(YDL169C)|FD-Score:3.5|P-value:2.37E-4||SGD DESC:Protein of unknown function, transcript accumulates in response to any combination of stress conditions Gene:URA1(YKL216W)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:Dihydroorotate dehydrogenase, catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid Gene:YAL066W(YAL066W_d)|FD-Score:3.34|P-value:4.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YAR029W(YAR029W_p)|FD-Score:3.28|P-value:5.24E-4||SGD DESC:Member of DUP240 gene family but contains no transmembrane domains; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YBL086C(YBL086C_p)|FD-Score:3.28|P-value:5.10E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YBL096C(YBL096C_d)|FD-Score:-3.35|P-value:4.06E-4||SGD DESC:Non-essential protein of unknown function Gene:YDL068W(YDL068W_d)|FD-Score:-3.1|P-value:9.57E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR008C(YDR008C_d)|FD-Score:15.8|P-value:1.33E-56||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR199W(YDR199W_d)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64; computationally predicted to have thiol-disulfide oxidoreductase activity Gene:YEL008W(YEL008W_d)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Hypothetical protein predicted to be involved in metabolism Gene:YER175W-A(YER175W-A_p)|FD-Score:-3.5|P-value:2.33E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YER188W(YER188W_d)|FD-Score:-3.67|P-value:1.21E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; large-scale analyses show mRNA expression increases under anaerobic conditions and two-hybrid interactions with Sst2p Gene:YFL012W(YFL012W_p)|FD-Score:5.74|P-value:4.67E-9||SGD DESC:Putative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin Gene:YFR035C(YFR035C_p)|FD-Score:3.92|P-value:4.45E-5||SGD DESC:Putative protein of unknown function, deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YGL015C(YGL015C_p)|FD-Score:9.86|P-value:3.23E-23||SGD DESC:Putative protein of unknown function; null mutants accumulate cargo in the Golgi Gene:YGL117W(YGL117W_p)|FD-Score:15.3|P-value:4.44E-53||SGD DESC:Putative protein of unknown function Gene:YHR035W(YHR035W_p)|FD-Score:-4.02|P-value:2.89E-5||SGD DESC:Putative protein of unknown function; not an essential gene Gene:YHR078W(YHR078W_p)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:High osmolarity-regulated gene of unknown function Gene:YJL064W(YJL064W_d)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YJL065C/DLS1 Gene:YML002W(YML002W_p)|FD-Score:-4.43|P-value:4.78E-6||SGD DESC:Putative protein of unknown function; expression induced by heat and by calcium shortage Gene:YML009C-A(YML009C-A_d)|FD-Score:-3.2|P-value:6.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL140C(YNL140C_p)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps THO2/YNL139C Gene:YNL276C(YNL276C_d)|FD-Score:3.16|P-value:7.84E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene MET2/YNL277W Gene:YPL162C(YPL162C_p)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of vacuole with cell cycle-correlated morphology Gene:YPT52(YKR014C)|FD-Score:3.13|P-value:8.64E-4||SGD DESC:Rab family GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; protein abundance increases in response to DNA replication stress Gene:YPT53(YNL093W)|FD-Score:5.33|P-value:4.87E-8||SGD DESC:Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication Gene:YTA7(YGR270W)|FD-Score:-3.42|P-value:3.13E-4||SGD DESC:Protein that localizes to chromatin and has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; potentially phosphorylated by Cdc28p Gene:AAC1(YMR056C)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; phosphorylated; Aac1p is a minor isoform while Pet9p is the major ADP/ATP translocator; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:AGE1(YDR524C)|FD-Score:3.79|P-value:7.61E-5||SGD DESC:ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in the secretory and endocytic pathways; contains C2C2H2 cysteine/histidine motif Gene:ANT1(YPR128C)|FD-Score:3.19|P-value:7.03E-4||SGD DESC:Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation Gene:ARG3(YJL088W)|FD-Score:3.11|P-value:9.28E-4||SGD DESC:Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine Gene:ARG5,6(YER069W)|FD-Score:7.38|P-value:7.96E-14||SGD DESC:Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine Gene:ARO1(YDR127W)|FD-Score:16.1|P-value:1.33E-58||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO2(YGL148W)|FD-Score:6.35|P-value:1.06E-10||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ARO3(YDR035W)|FD-Score:14.3|P-value:5.81E-47||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ASH1(YKL185W)|FD-Score:-3.11|P-value:9.23E-4||SGD DESC:Zinc-finger inhibitor of HO transcription; mRNA is localized and translated in the distal tip of anaphase cells, resulting in accumulation of Ash1p in daughter cell nuclei and inhibition of HO expression; potential Cdc28p substrate Gene:ASR1(YPR093C)|FD-Score:4.35|P-value:6.73E-6||SGD DESC:Ubiquitin ligase that modifies and regulates RNA Pol II; involved in a putative alcohol-responsive signaling pathway; accumulates in the nucleus under alcohol stress; contains a Ring/PHD finger domain similar to the mammalian rA9 protein Gene:BIT2(YBR270C_p)|FD-Score:4.15|P-value:1.63E-5||SGD DESC:Subunit of TORC2 membrane-associated complex; involved in regulation of actin cytoskeletal dynamics during polarized growth and cell wall integrity; interacts with Slm1p and Slm2p, homologous PH domain-containing TORC2 substrates; BIT2 has a paralog, BIT61, that arose from the whole genome duplication Gene:BUD21(YOR078W)|FD-Score:-4.59|P-value:2.21E-6||SGD DESC:Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated as bud-site selection mutant that displays a random budding pattern Gene:CAR1(YPL111W)|FD-Score:7.71|P-value:6.53E-15||SGD DESC:Arginase, responsible for arginine degradation, expression responds to both induction by arginine and nitrogen catabolite repression; disruption enhances freeze tolerance Gene:CIR2(YOR356W)|FD-Score:7.82|P-value:2.65E-15||SGD DESC:Putative ortholog of human electron transfer flavoprotein dehydrogenase (ETF-dH); found in a large supramolecular complex with other mitochondrial dehydrogenases; may have a role in oxidative stress response Gene:COG7(YGL005C)|FD-Score:3.2|P-value:6.88E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:4.32|P-value:7.90E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CRN1(YLR429W)|FD-Score:4.93|P-value:4.20E-7||SGD DESC:Coronin, cortical actin cytoskeletal component that associates with the Arp2p/Arp3p complex to regulate its activity; plays a role in regulation of actin patch assembly Gene:CTH1(YDR151C)|FD-Score:-3.57|P-value:1.82E-4||SGD DESC:Member of the CCCH zinc finger family; has similarity to mammalian Tis11 protein, which activates transcription and also has a role in mRNA degradation; may function with Tis11p in iron homeostasis Gene:DIC1(YLR348C)|FD-Score:-4.3|P-value:8.39E-6||SGD DESC:Mitochondrial dicarboxylate carrier, integral membrane protein, catalyzes a dicarboxylate-phosphate exchange across the inner mitochondrial membrane, transports cytoplasmic dicarboxylates into the mitochondrial matrix Gene:DUF1(YOL087C)|FD-Score:3.2|P-value:6.93E-4||SGD DESC:Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid Gene:ECM33(YBR078W)|FD-Score:-4.25|P-value:1.08E-5||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:FAR3(YMR052W)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:FEN2(YCR028C)|FD-Score:-9.06|P-value:6.25E-20||SGD DESC:Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:GCN2(YDR283C)|FD-Score:8.33|P-value:3.91E-17||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN20(YFR009W)|FD-Score:5.16|P-value:1.25E-7||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:12.4|P-value:1.23E-35||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:25.3|P-value:1.67E-141||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GGA1(YDR358W)|FD-Score:3.84|P-value:6.18E-5||SGD DESC:Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi Gene:GIP1(YBR045C)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:Meiosis-specific regulatory subunit of the Glc7p protein phosphatase, regulates spore wall formation and septin organization, required for expression of some late meiotic genes and for normal localization of Glc7p Gene:GPD1(YDL022W)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity Gene:GPD2(YOL059W)|FD-Score:7.54|P-value:2.42E-14||SGD DESC:NAD-dependent glycerol 3-phosphate dehydrogenase, homolog of Gpd1p, expression is controlled by an oxygen-independent signaling pathway required to regulate metabolism under anoxic conditions; located in cytosol and mitochondria; constitutively active but is inactivated via phosphorylation by energy-stress responsive kinase SNF1 Gene:GPT2(YKR067W)|FD-Score:4.51|P-value:3.25E-6||SGD DESC:Glycerol-3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen Gene:GSF2(YML048W)|FD-Score:4.71|P-value:1.27E-6||SGD DESC:ER localized integral membrane protein that may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression Gene:HNT3(YOR258W)|FD-Score:-3.29|P-value:5.01E-4||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:HOR7(YMR251W-A)|FD-Score:-3.21|P-value:6.60E-4||SGD DESC:Protein of unknown function; overexpression suppresses Ca2+ sensitivity of mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p; transcription is induced under hyperosmotic stress and repressed by alpha factor; HOR7 has a paralog, DDR2, that arose from the whole genome duplication Gene:HTD2(YHR067W)|FD-Score:-4.94|P-value:3.84E-7||SGD DESC:Mitochondrial 3-hydroxyacyl-thioester dehydratase involved in fatty acid biosynthesis, required for respiratory growth and for normal mitochondrial morphology Gene:IDP2(YLR174W)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:Cytosolic NADP-specific isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate; levels are elevated during growth on non-fermentable carbon sources and reduced during growth on glucose; IDP2 has a paralog, IDP3, that arose from the whole genome duplication Gene:INP51(YIL002C)|FD-Score:3.34|P-value:4.25E-4||SGD DESC:Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth Gene:IRC16(YPR038W_d)|FD-Score:3.17|P-value:7.54E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene YPR037C; null mutant displays increased levels of spontaneous Rad52p foci Gene:ISU1(YPL135W)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Conserved protein of the mitochondrial matrix, performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable Gene:KAP123(YER110C)|FD-Score:-3.27|P-value:5.42E-4||SGD DESC:Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1 Gene:KIN2(YLR096W)|FD-Score:3.12|P-value:9.17E-4||SGD DESC:Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p Gene:LRO1(YNR008W)|FD-Score:4.02|P-value:2.93E-5||SGD DESC:Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase Gene:LSB5(YCL034W)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Protein of unknown function; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; may mediate disassembly of the Pan1 complex from the endocytic coat Gene:MDM34(YGL219C)|FD-Score:3.37|P-value:3.72E-4||SGD DESC:Mitochondrial component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MET17(YLR303W)|FD-Score:6.39|P-value:8.13E-11||SGD DESC:Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis Gene:MET3(YJR010W)|FD-Score:-3.22|P-value:6.32E-4||SGD DESC:ATP sulfurylase, catalyzes the primary step of intracellular sulfate activation, essential for assimilatory reduction of sulfate to sulfide, involved in methionine metabolism Gene:MF(ALPHA)2(YGL089C)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:Mating pheromone alpha-factor, made by alpha cells; interacts with mating type a cells to induce cell cycle arrest and other responses leading to mating; also encoded by MF(ALPHA)1, which is more highly expressed than MF(ALPHA)2 Gene:MMS2(YGL087C)|FD-Score:-3.13|P-value:8.84E-4||SGD DESC:Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress Gene:MNR2(YKL064W)|FD-Score:4.48|P-value:3.73E-6||SGD DESC:Vacuolar membrane protein required for magnesium homeostasis; putative magnesium transporter; has similarity to Alr1p and Alr2p, which mediate influx of Mg2+ and other divalent cations Gene:MSO1(YNR049C)|FD-Score:3.31|P-value:4.68E-4||SGD DESC:Probable component of the secretory vesicle docking complex; acts at a late step in secretion; shows genetic and physical interactions with Sec1p; required for prospore membrane formation during sporulation; relocalizes from bud neck to nucleus upon DNA replication stress Gene:MSY1(YPL097W)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Mitochondrial tyrosyl-tRNA synthetase Gene:NIT3(YLR351C)|FD-Score:3.81|P-value:6.85E-5||SGD DESC:Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member Gene:NMA111(YNL123W)|FD-Score:4.49|P-value:3.64E-6||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:NOP6(YDL213C)|FD-Score:-3.16|P-value:7.91E-4||SGD DESC:rRNA-binding protein required for 40S ribosomal subunit biogenesis; contains an RNA recognition motif (RRM); hydrophilin essential to overcome the stress of the desiccation-rehydration process; NOP6 may be a fungal-specific gene as no homologs have been yet identified in higher eukaryotes Gene:NUP170(YBL079W)|FD-Score:-3.92|P-value:4.52E-5||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication Gene:OPI3(YJR073C)|FD-Score:3.89|P-value:5.08E-5||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:ORT1(YOR130C)|FD-Score:3.95|P-value:3.84E-5||SGD DESC:Ornithine transporter of the mitochondrial inner membrane, exports ornithine from mitochondria as part of arginine biosynthesis; human ortholog is associated with hyperammonaemia-hyperornithinaemia-homocitrullinuria (HHH) syndrome Gene:PDR1(YGL013C)|FD-Score:-3.32|P-value:4.54E-4||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PDR11(YIL013C)|FD-Score:3.11|P-value:9.33E-4||SGD DESC:ATP-binding cassette (ABC) transporter, multidrug transporter involved in multiple drug resistance; mediates sterol uptake when sterol biosynthesis is compromised; regulated by Pdr1p; required for anaerobic growth Gene:PDR16(YNL231C)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress Gene:PEX1(YKL197C)|FD-Score:-3.84|P-value:6.05E-5||SGD DESC:AAA-peroxin that heterodimerizes with AAA-peroxin Pex6p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol; induced by oleic acid and upregulated during anaerobiosis Gene:PKH2(YOL100W)|FD-Score:-3.62|P-value:1.46E-4||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p Gene:PKP2(YGL059W)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Mitochondrial protein kinase; negatively regulates activity of the pyruvate dehydrogenase complex by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp1p and phosphatases Ptc5p and Ptc6p; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:POP2(YNR052C)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PRM8(YGL053W)|FD-Score:4.42|P-value:4.92E-6||SGD DESC:Pheromone-regulated protein with 2 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; forms a complex with Prp9p in the ER; member of DUP240 gene family Gene:ROM2(YLR371W)|FD-Score:-6.14|P-value:4.19E-10||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL22B(YFL034C-A)|FD-Score:4|P-value:3.14E-5||SGD DESC:Ribosomal 60S subunit protein L22B; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22B has a paralog, RPL22A, that arose from the whole genome duplication Gene:RRG7(YOR305W)|FD-Score:-3.24|P-value:5.88E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YOR305W is not an essential gene Gene:RTC6(YPL183W-A)|FD-Score:-3.26|P-value:5.65E-4||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:SAC6(YDR129C)|FD-Score:-3.12|P-value:9.13E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SAN1(YDR143C)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Ubiquitin-protein ligase; involved in the proteasome-dependent degradation of aberrant nuclear proteins; targets substrates with regions of exposed hydrophobicity containing 5 or more contiguous hydrophobic residues; contains intrinsically disordered regions that contribute to substrate recognition Gene:SLG1(YOR008C)|FD-Score:-3.29|P-value:5.09E-4||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SNF12(YNR023W)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive Gene:SPG3(YDR504C)|FD-Score:-3.5|P-value:2.34E-4||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources Gene:SRT1(YMR101C)|FD-Score:4.11|P-value:2.00E-5||SGD DESC:Cis-prenyltransferase involved in synthesis of long-chain dolichols (19-22 isoprene units; as opposed to Rer2p which synthesizes shorter-chain dolichols); localizes to lipid bodies; transcription is induced during stationary phase Gene:STB6(YKL072W)|FD-Score:-3.55|P-value:1.91E-4||SGD DESC:Protein that binds Sin3p in a two-hybrid assay Gene:SVF1(YDR346C)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Protein with a potential role in cell survival pathways, required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance Gene:SVP26(YHR181W)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Integral membrane protein of the early Golgi apparatus and endoplasmic reticulum, involved in COP II vesicle transport; may also function to promote retention of proteins in the early Golgi compartment Gene:SWC7(YLR385C)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:Protein of unknown function, component of the Swr1p complex that incorporates Htz1p into chromatin Gene:TAT1(YBR069C)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TDA11(YHR159W_p)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele Gene:TMS1(YDR105C)|FD-Score:4.73|P-value:1.15E-6||SGD DESC:Vacuolar membrane protein of unknown function that is conserved in mammals; predicted to contain eleven transmembrane helices; interacts with Pdr5p, a protein involved in multidrug resistance Gene:TRE1(YPL176C)|FD-Score:4.3|P-value:8.72E-6||SGD DESC:Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitylation and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication Gene:TRP1(YDR007W)|FD-Score:21.8|P-value:3.32E-105||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:22.4|P-value:1.17E-111||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:27.1|P-value:1.29E-161||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:24.7|P-value:2.50E-135||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:10.5|P-value:4.98E-26||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TSR2(YLR435W)|FD-Score:-3.29|P-value:5.09E-4||SGD DESC:Protein with a potential role in pre-rRNA processing Gene:UBP3(YER151C)|FD-Score:-3.25|P-value:5.70E-4||SGD DESC:Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress Gene:UBP5(YER144C)|FD-Score:3.12|P-value:8.97E-4||SGD DESC:Putative ubiquitin-specific protease, closest paralog of Doa4p but has no functional overlap; concentrates at the bud neck Gene:UBP6(YFR010W)|FD-Score:-3.46|P-value:2.72E-4||SGD DESC:Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance Gene:UGX2(YDL169C)|FD-Score:3.5|P-value:2.37E-4||SGD DESC:Protein of unknown function, transcript accumulates in response to any combination of stress conditions Gene:URA1(YKL216W)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:Dihydroorotate dehydrogenase, catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid Gene:YAL066W(YAL066W_d)|FD-Score:3.34|P-value:4.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YAR029W(YAR029W_p)|FD-Score:3.28|P-value:5.24E-4||SGD DESC:Member of DUP240 gene family but contains no transmembrane domains; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YBL086C(YBL086C_p)|FD-Score:3.28|P-value:5.10E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YBL096C(YBL096C_d)|FD-Score:-3.35|P-value:4.06E-4||SGD DESC:Non-essential protein of unknown function Gene:YDL068W(YDL068W_d)|FD-Score:-3.1|P-value:9.57E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR008C(YDR008C_d)|FD-Score:15.8|P-value:1.33E-56||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR199W(YDR199W_d)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64; computationally predicted to have thiol-disulfide oxidoreductase activity Gene:YEL008W(YEL008W_d)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Hypothetical protein predicted to be involved in metabolism Gene:YER175W-A(YER175W-A_p)|FD-Score:-3.5|P-value:2.33E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YER188W(YER188W_d)|FD-Score:-3.67|P-value:1.21E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; large-scale analyses show mRNA expression increases under anaerobic conditions and two-hybrid interactions with Sst2p Gene:YFL012W(YFL012W_p)|FD-Score:5.74|P-value:4.67E-9||SGD DESC:Putative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin Gene:YFR035C(YFR035C_p)|FD-Score:3.92|P-value:4.45E-5||SGD DESC:Putative protein of unknown function, deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YGL015C(YGL015C_p)|FD-Score:9.86|P-value:3.23E-23||SGD DESC:Putative protein of unknown function; null mutants accumulate cargo in the Golgi Gene:YGL117W(YGL117W_p)|FD-Score:15.3|P-value:4.44E-53||SGD DESC:Putative protein of unknown function Gene:YHR035W(YHR035W_p)|FD-Score:-4.02|P-value:2.89E-5||SGD DESC:Putative protein of unknown function; not an essential gene Gene:YHR078W(YHR078W_p)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:High osmolarity-regulated gene of unknown function Gene:YJL064W(YJL064W_d)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YJL065C/DLS1 Gene:YML002W(YML002W_p)|FD-Score:-4.43|P-value:4.78E-6||SGD DESC:Putative protein of unknown function; expression induced by heat and by calcium shortage Gene:YML009C-A(YML009C-A_d)|FD-Score:-3.2|P-value:6.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL140C(YNL140C_p)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps THO2/YNL139C Gene:YNL276C(YNL276C_d)|FD-Score:3.16|P-value:7.84E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene MET2/YNL277W Gene:YPL162C(YPL162C_p)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of vacuole with cell cycle-correlated morphology Gene:YPT52(YKR014C)|FD-Score:3.13|P-value:8.64E-4||SGD DESC:Rab family GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; protein abundance increases in response to DNA replication stress Gene:YPT53(YNL093W)|FD-Score:5.33|P-value:4.87E-8||SGD DESC:Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication Gene:YTA7(YGR270W)|FD-Score:-3.42|P-value:3.13E-4||SGD DESC:Protein that localizes to chromatin and has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; potentially phosphorylated by Cdc28p

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YCL041C_d6.719.48E-120.96YCL041C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps both the verified gene PDI1/YCL043C and the uncharacterized gene YCL042W
YNL282W5.813.14E-90.96POP3Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YML077W5.219.39E-80.96BET5Component of the TRAPP (transport protein particle) complex, which plays an essential role in the vesicular transport from endoplasmic reticulum to Golgi
YJL026W4.261.04E-50.11RNR2Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits
YJL174W4.151.68E-50.09KRE9Glycoprotein involved in cell wall beta-glucan assembly; null mutation leads to severe growth defects, aberrant multibudded morphology, and mating defects
YDR324C4.062.48E-50.09UTP4Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript
YBR265W3.973.57E-50.09TSC103-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family
YGL055W3.885.14E-50.14OLE1Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
YDR412W3.758.87E-50.25RRP17Component of the pre-60S pre-ribosomal particle; required for cell viability under standard (aerobic) conditions but not under anaerobic conditions; exonuclease required for 5′ end processing of pre-60S ribosomal RNA
YMR094W3.502.36E-40.03CTF13Subunit of the CBF3 complex, which binds to the CDE III element of centromeres, bending the DNA upon binding, and may be involved in sister chromatid cohesion during mitosis
YPL252C3.472.64E-40.00YAH1Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin
YNL161W3.462.66E-40.03CBK1Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress
YAL041W3.433.02E-40.06CDC24Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress
YGL068W3.373.82E-40.02MNP1Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth
YPL083C3.354.08E-40.01SEN54Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKL211C27.101.29E-161TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YEL009C25.301.67E-141GCN4Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
YDR354W24.702.50E-135TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YER090W22.401.17E-111TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YDR007W21.803.32E-105TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YDR127W16.101.33E-58ARO1Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
YDR008C_d15.801.33E-56YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YGL117W_p15.304.44E-53YGL117W_pPutative protein of unknown function
YDR035W14.305.81E-47ARO33-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan
YKR026C12.401.23E-35GCN3Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression
YGL026C10.504.98E-26TRP5Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis
YGL015C_p9.863.23E-23YGL015C_pPutative protein of unknown function; null mutants accumulate cargo in the Golgi
YDR283C8.333.91E-17GCN2Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control
YOR356W7.822.65E-15CIR2Putative ortholog of human electron transfer flavoprotein dehydrogenase (ETF-dH); found in a large supramolecular complex with other mitochondrial dehydrogenases; may have a role in oxidative stress response
YPL111W7.716.53E-15CAR1Arginase, responsible for arginine degradation, expression responds to both induction by arginine and nitrogen catabolite repression; disruption enhances freeze tolerance

GO enrichment analysis for SGTC_2758
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.6250SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592471
0.5920SGTC_18825615643 20.0 μMMiscellaneous22530750.060241TRP & mitochondrial translation
0.5820SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.0526316
0.5730SGTC_1697st025554 27.7 μMTimTec (Natural product derivative library)12695190.0735294
0.5720SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.1
0.5720SGTC_1572epiandrosterone 68.9 μMTimTec (Pure natural product library)4413020.52
0.5670SGTC_1801486-1293 102.8 μMChemDiv (Drug-like library)41405360.0487805
0.5480SGTC_1649st011737 51.3 μMTimTec (Natural product derivative library)17610350.038961
0.5470SGTC_511013-0126 23.4 μMChemDiv (Drug-like library)16278500.0533333
0.5470SGTC_23849036245 200.0 μMChembridge (Fragment library)64627660.0757576
0.5460SGTC_18815652484 16.0 μMMiscellaneous22551290.0609756TRP & mitochondrial translation
0.5450SGTC_24885268135 45.7 μMMiscellaneous12703560.0606061
0.5420SGTC_2669eugenol 100.0 μMMicrosource (Natural product library)33140.0645161
0.5340SGTC_18835-benzylidenerhodanine 20.0 μMMiscellaneous12732100.0645161TRP & mitochondrial translation
0.5310SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.181818

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2650prasterone100 μM116759247Microsource (Natural product library)288.424423.33812
SGTC_1757st04162031.84 μM0.5535715289815TimTec (Natural product derivative library)329.476342.42223
SGTC_1715st03216010.23 μM0.5344835289825TimTec (Natural product derivative library)371.513022.21813
SGTC_1572epiandrosterone68.9 μM0.52441302TimTec (Pure natural product library)290.44033.58812
SGTC_2540epiandrosterone100 μM0.52441302TimTec (Pure natural product library)290.44033.58812
SGTC_2584androsterone48.74 μM0.522754130Microsource (Natural product library)290.44033.58812cell wall
SGTC_2607solasodine47.35 μM0.4615386710642Microsource (Natural product library)413.635824.36723
SGTC_25005-alpha-androstan-3,17-dione61.44 μM0.450986710655Microsource (Natural product library)288.424423.40202
SGTC_2074185-00104.49 μM0.4477613761753ChemDiv (Drug-like library)427.66245.12413endomembrane recycling
SGTC_406estrone100 μM0.4107145870Miscellaneous270.366083.93812
SGTC_1519esterone74 μM0.410714698TimTec (Pure natural product library)270.366083.93812DNA intercalators