levamisole FDA approved compound

(6S)-6-phenyl-2,3,5,6-tetrahydroimidazo[2,1-b][1,3]thiazole

An alkaline phosphatase inhibitor.

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PubChem MeSH terms: Adjuvants, Immunologic;Antinematodal Agents;Antirheumatic Agents



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2761
Screen concentration 97.9 μM
Source Miscellaneous
PubChem CID 26879
SMILES C1CSC2=NC(CN21)C3=CC=CC=C3
Standardized SMILES C1CN2CC(N=C2S1)c3ccccc3
Molecular weight 204.2914
ALogP 2.29
H-bond donor count 0
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.39
% growth inhibition (Hom. pool) 3.43


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 26879
Download HIP data (tab-delimited text)  (excel)
Gene:ATP16(YDL004W)|FD-Score:4.24|P-value:1.10E-5|Clearance:0.61||SGD DESC:Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:FOL2(YGR267C)|FD-Score:5.21|P-value:9.39E-8|Clearance:0.61||SGD DESC:GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway Gene:GPI18(YBR004C)|FD-Score:3.29|P-value:5.05E-4|Clearance:0.27||SGD DESC:Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria Gene:GPM1(YKL152C)|FD-Score:-3.24|P-value:5.96E-4|Clearance:0||SGD DESC:Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis Gene:NMD3(YHR170W)|FD-Score:-3.89|P-value:4.97E-5|Clearance:0||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NSE3(YDR288W)|FD-Score:-3.4|P-value:3.35E-4|Clearance:0||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress Gene:RPN3(YER021W)|FD-Score:3.9|P-value:4.86E-5|Clearance:0.61||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:RPP1(YHR062C)|FD-Score:-4.27|P-value:9.83E-6|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SMC4(YLR086W)|FD-Score:-3.55|P-value:1.94E-4|Clearance:0||SGD DESC:Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate Gene:TIF34(YMR146C)|FD-Score:4.63|P-value:1.84E-6|Clearance:0.61||SGD DESC:eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation Gene:YDR526C(YDR526C_d)|FD-Score:-4.07|P-value:2.39E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ATP16(YDL004W)|FD-Score:4.24|P-value:1.10E-5|Clearance:0.61||SGD DESC:Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:FOL2(YGR267C)|FD-Score:5.21|P-value:9.39E-8|Clearance:0.61||SGD DESC:GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway Gene:GPI18(YBR004C)|FD-Score:3.29|P-value:5.05E-4|Clearance:0.27||SGD DESC:Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria Gene:GPM1(YKL152C)|FD-Score:-3.24|P-value:5.96E-4|Clearance:0||SGD DESC:Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis Gene:NMD3(YHR170W)|FD-Score:-3.89|P-value:4.97E-5|Clearance:0||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NSE3(YDR288W)|FD-Score:-3.4|P-value:3.35E-4|Clearance:0||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress Gene:RPN3(YER021W)|FD-Score:3.9|P-value:4.86E-5|Clearance:0.61||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:RPP1(YHR062C)|FD-Score:-4.27|P-value:9.83E-6|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SMC4(YLR086W)|FD-Score:-3.55|P-value:1.94E-4|Clearance:0||SGD DESC:Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate Gene:TIF34(YMR146C)|FD-Score:4.63|P-value:1.84E-6|Clearance:0.61||SGD DESC:eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation Gene:YDR526C(YDR526C_d)|FD-Score:-4.07|P-value:2.39E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 26879
Download HOP data (tab-delimited text)  (excel)
Gene:ADH5(YBR145W)|FD-Score:3.87|P-value:5.49E-5||SGD DESC:Alcohol dehydrogenase isoenzyme V; involved in ethanol production; ADH5 has a paralog, ADH1, that arose from the whole genome duplication Gene:AIM4(YBR194W)|FD-Score:3.37|P-value:3.79E-4||SGD DESC:Protein proposed to be associated with the nuclear pore complex; null mutant is viable, displays elevated frequency of mitochondrial genome loss and is sensitive to freeze-thaw stress Gene:APL5(YPL195W)|FD-Score:-3.56|P-value:1.88E-4||SGD DESC:Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function Gene:ASH1(YKL185W)|FD-Score:3.24|P-value:5.92E-4||SGD DESC:Zinc-finger inhibitor of HO transcription; mRNA is localized and translated in the distal tip of anaphase cells, resulting in accumulation of Ash1p in daughter cell nuclei and inhibition of HO expression; potential Cdc28p substrate Gene:AST1(YBL069W)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Lipid raft associated protein; interacts with the plasma membrane ATPase Pma1p and has a role in its targeting to the plasma membrane by influencing its incorporation into lipid rafts; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST1 has a paralog, AST2, that arose from the whole genome duplication Gene:ATS1(YAL020C)|FD-Score:4.34|P-value:7.02E-6||SGD DESC:Protein required, with Elongator complex, Kti11p, and Kti12p, for modification of wobble nucleosides in tRNA; has a potential role in regulatory interactions between microtubules and the cell cycle Gene:BIT61(YJL058C)|FD-Score:4.16|P-value:1.59E-5||SGD DESC:Subunit of TORC2 membrane-associated complex; involved in regulation of cell cycle-dependent actin cytoskeletal dynamics during polarized growth and cell wall integrity; BIT61 has a paralog, BIT2, that arose from the whole genome duplication Gene:BUD25(YER014C-A)|FD-Score:-3.85|P-value:5.85E-5||SGD DESC:Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern Gene:CRH1(YGR189C)|FD-Score:3.78|P-value:7.75E-5||SGD DESC:Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar and functionally redundant to Utr2; localizes to sites of polarized growth; expression induced by cell wall stress Gene:CWC27(YPL064C)|FD-Score:-3.58|P-value:1.70E-4||SGD DESC:Component of a complex containing Cef1p; putatively involved in pre-mRNA splicing; has similarity to S. pombe Cwf27p; protein abundance increases in response to DNA replication stress Gene:DOA4(YDR069C)|FD-Score:-3.33|P-value:4.33E-4||SGD DESC:Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole Gene:ECM2(YBR065C)|FD-Score:3.8|P-value:7.33E-5||SGD DESC:Pre-mRNA splicing factor, facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p Gene:EGT2(YNL327W)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Glycosylphosphatidylinositol (GPI)-anchored cell wall endoglucanase required for proper cell separation after cytokinesis, expression is activated by Swi5p and tightly regulated in a cell cycle-dependent manner Gene:FYV10(YIL097W)|FD-Score:3.94|P-value:4.14E-5||SGD DESC:Subunit of GID complex; involved in proteasome-dependent catabolite inactivation of gluconeogenic enzymes FBPase, PEPCK, and c-MDH; forms dimer with Rmd5p that is then recruited to GID Complex by Gid8p; contains a degenerate RING finger motif needed for GID complex ubiquitin ligase activity in vivo, as well as CTLH and CRA domains; plays role in anti-apoptosis; required for survival upon exposure to K1 killer toxin Gene:GLN3(YER040W)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:Transcriptional activator of genes regulated by nitrogen catabolite repression (NCR), localization and activity regulated by quality of nitrogen source Gene:HMS1(YOR032C)|FD-Score:3.76|P-value:8.56E-5||SGD DESC:Basic helix-loop-helix (bHLH) protein with similarity to myc-family transcription factors; overexpression confers hyperfilamentous growth and suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant Gene:HOF1(YMR032W)|FD-Score:4.52|P-value:3.14E-6||SGD DESC:Bud neck-localized, SH3 domain-containing protein required for cytokinesis; is phosphorylated by Dbf2p; regulates actomyosin ring dynamics and septin localization; interacts with the formins, Bni1p and Bnr1p, and with Cyk3p, Vrp1p, and Bni5p Gene:HOT13(YKL084W)|FD-Score:-3.11|P-value:9.37E-4||SGD DESC:Zinc-binding mitochondrial intermembrane space (IMS) protein involved in a disulfide relay system for IMS import of cysteine-containing proteins; binds Mia40p and stimulates its Erv1p-dependent oxidation, probably by sequestering zinc Gene:ICE2(YIL090W)|FD-Score:-3.81|P-value:6.87E-5||SGD DESC:Integral ER membrane protein with type-III transmembrane domains; required for maintenance of ER zinc homeostasis; necessary for efficient targeting of Trm1p tRNA methyltransferase to inner nuclear membrane; mutations cause defects in cortical ER morphology in both the mother and daughter cells Gene:LSB6(YJL100W)|FD-Score:3.13|P-value:8.62E-4||SGD DESC:Type II phosphatidylinositol 4-kinase that binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization Gene:MCK1(YNL307C)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Protein serine/threonine/tyrosine (dual-specificity) kinase; involved in control of chromosome segregation and in regulating entry into meiosis; interacts with Clb2p and Mih1p; inhibits Clb2p-Cdk1 activity; related to mammalian glycogen synthase kinases of the GSK-3 family Gene:MCM21(YDR318W)|FD-Score:3.17|P-value:7.75E-4||SGD DESC:Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2 Gene:MSN2(YMR037C)|FD-Score:-3.45|P-value:2.83E-4||SGD DESC:Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN2 has a paralog, MSN4, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:NPP2(YEL016C)|FD-Score:5.03|P-value:2.46E-7||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp1p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP2 has a paralog, NPP1, that arose from the whole genome duplication Gene:OMA1(YKR087C)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Metalloendopeptidase of the mitochondrial inner membrane; involved in turnover of membrane-embedded proteins; mediates degradation of Cox1p in coa2 mutant cells; member of a family of predicted membrane-bound metallopeptidases in prokaryotes and higher eukaryotes Gene:OMS1(YDR316W)|FD-Score:3.21|P-value:6.68E-4||SGD DESC:Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations Gene:OSH2(YDL019C)|FD-Score:3.29|P-value:4.97E-4||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PDH1(YPR002W)|FD-Score:5.01|P-value:2.71E-7||SGD DESC:Mitochondrial protein that participates in respiration, induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate Gene:PFK2(YMR205C)|FD-Score:3.21|P-value:6.54E-4||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PHA2(YNL316C)|FD-Score:3.12|P-value:9.18E-4||SGD DESC:Prephenate dehydratase, catalyzes the conversion of prephanate to phenylpyruvate, which is a step in the phenylalanine biosynthesis pathway Gene:PTC4(YBR125C)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Cytoplasmic type 2C protein phosphatase (PP2C); identified as a high-copy number suppressor of cnb1 mpk1 synthetic lethality; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity Gene:PTP2(YOR208W)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the nucleus Gene:RAD6(YGL058W)|FD-Score:-3.56|P-value:1.88E-4||SGD DESC:Ubiquitin-conjugating enzyme (E2), involved in postreplication repair (as a heterodimer with Rad18p), DSBR and checkpoint control (as a heterodimer with Bre1p), ubiquitin-mediated N-end rule protein degradation (as a heterodimer with Ubr1p Gene:RPL26A(YLR344W)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Ribosomal 60S subunit protein L26A; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26A has a paralog, RPL26B, that arose from the whole genome duplication Gene:RPL42B(YHR141C)|FD-Score:-3.71|P-value:1.04E-4||SGD DESC:Ribosomal 60S subunit protein L42B; required for propagation of the killer toxin-encoding M1 double-stranded RNA satellite of the L-A double-stranded RNA virus; homologous to mammalian ribosomal protein L36A, no bacterial homolog; RPL42B has a paralog, RPL42A, that arose from the whole genome duplication Gene:RPL4A(YBR031W)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Ribosomal 60S subunit protein L4A; N-terminally acetylated; homologous to mammalian ribosomal protein L4 and bacterial L4; RPL4A has a paralog, RPL4B, that arose from the whole genome duplication Gene:RPS18B(YML026C)|FD-Score:4.78|P-value:8.95E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18B has a paralog, RPS18A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SET5(YHR207C)|FD-Score:-3.34|P-value:4.15E-4||SGD DESC:Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus Gene:SNQ2(YDR011W)|FD-Score:-3.12|P-value:9.11E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SPR6(YER115C)|FD-Score:3.26|P-value:5.65E-4||SGD DESC:Protein of unknown function, expressed during sporulation; not required for sporulation, but gene exhibits genetic interactions with other genes required for sporulation Gene:SPS18(YNL204C)|FD-Score:5.73|P-value:5.08E-9||SGD DESC:Protein of unknown function, contains a putative zinc-binding domain; expressed during sporulation Gene:TDA5(YLR426W_p)|FD-Score:-5.51|P-value:1.79E-8||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:TDH2(YJR009C)|FD-Score:-3.35|P-value:4.02E-4||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress; TDH2 has a paralog, TDH3, that arose from the whole genome duplication Gene:TES1(YJR019C)|FD-Score:3.98|P-value:3.42E-5||SGD DESC:Peroxisomal acyl-CoA thioesterase likely to be involved in fatty acid oxidation rather than fatty acid synthesis; conserved protein also found in human peroxisomes; TES1 mRNA levels increase during growth on fatty acids Gene:TGS1(YPL157W)|FD-Score:4.48|P-value:3.78E-6||SGD DESC:Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs Gene:TRP2(YER090W)|FD-Score:-3.48|P-value:2.48E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:-3.96|P-value:3.70E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TYW3(YGL050W)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:tRNA methyltransferase required for synthesis of wybutosine, a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions Gene:UGA2(YBR006W)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:URE2(YNL229C)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VAC17(YCL063W)|FD-Score:-3.98|P-value:3.40E-5||SGD DESC:Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p Gene:VPS55(YJR044C)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP) Gene:YAL018C(YAL018C_p)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Putative protein of unknown function Gene:YAP5(YIR018W)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Basic leucine zipper (bZIP) iron-sensing transcription factor; YAP5 has a paralog, YAP7, that arose from the whole genome duplication Gene:YCL049C(YCL049C_p)|FD-Score:3.61|P-value:1.50E-4||SGD DESC:Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene Gene:YDR271C(YDR271C_d)|FD-Score:-3.11|P-value:9.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W Gene:YDR444W(YDR444W_p)|FD-Score:5.25|P-value:7.45E-8||SGD DESC:Putative hydrolase acting on ester bonds Gene:YGR160W(YGR160W_d)|FD-Score:-4.12|P-value:1.86E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR273C(YGR273C_p)|FD-Score:4.11|P-value:1.98E-5||SGD DESC:Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene Gene:YGR290W(YGR290W_d)|FD-Score:5.26|P-value:7.23E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) Gene:YIM2(YMR151W_d)|FD-Score:4.43|P-value:4.74E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1 Gene:YMR209C(YMR209C_p)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; YMR209C is not an essential gene Gene:YNL122C(YNL122C_p)|FD-Score:3.76|P-value:8.50E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL122C is not an essential gene Gene:YPK1(YKL126W)|FD-Score:-3.58|P-value:1.74E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL039W(YPL039W_p)|FD-Score:3.15|P-value:8.15E-4||SGD DESC:Putative protein of unknown function; YPL039W is not an essential gene Gene:ADH5(YBR145W)|FD-Score:3.87|P-value:5.49E-5||SGD DESC:Alcohol dehydrogenase isoenzyme V; involved in ethanol production; ADH5 has a paralog, ADH1, that arose from the whole genome duplication Gene:AIM4(YBR194W)|FD-Score:3.37|P-value:3.79E-4||SGD DESC:Protein proposed to be associated with the nuclear pore complex; null mutant is viable, displays elevated frequency of mitochondrial genome loss and is sensitive to freeze-thaw stress Gene:APL5(YPL195W)|FD-Score:-3.56|P-value:1.88E-4||SGD DESC:Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function Gene:ASH1(YKL185W)|FD-Score:3.24|P-value:5.92E-4||SGD DESC:Zinc-finger inhibitor of HO transcription; mRNA is localized and translated in the distal tip of anaphase cells, resulting in accumulation of Ash1p in daughter cell nuclei and inhibition of HO expression; potential Cdc28p substrate Gene:AST1(YBL069W)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Lipid raft associated protein; interacts with the plasma membrane ATPase Pma1p and has a role in its targeting to the plasma membrane by influencing its incorporation into lipid rafts; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST1 has a paralog, AST2, that arose from the whole genome duplication Gene:ATS1(YAL020C)|FD-Score:4.34|P-value:7.02E-6||SGD DESC:Protein required, with Elongator complex, Kti11p, and Kti12p, for modification of wobble nucleosides in tRNA; has a potential role in regulatory interactions between microtubules and the cell cycle Gene:BIT61(YJL058C)|FD-Score:4.16|P-value:1.59E-5||SGD DESC:Subunit of TORC2 membrane-associated complex; involved in regulation of cell cycle-dependent actin cytoskeletal dynamics during polarized growth and cell wall integrity; BIT61 has a paralog, BIT2, that arose from the whole genome duplication Gene:BUD25(YER014C-A)|FD-Score:-3.85|P-value:5.85E-5||SGD DESC:Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern Gene:CRH1(YGR189C)|FD-Score:3.78|P-value:7.75E-5||SGD DESC:Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar and functionally redundant to Utr2; localizes to sites of polarized growth; expression induced by cell wall stress Gene:CWC27(YPL064C)|FD-Score:-3.58|P-value:1.70E-4||SGD DESC:Component of a complex containing Cef1p; putatively involved in pre-mRNA splicing; has similarity to S. pombe Cwf27p; protein abundance increases in response to DNA replication stress Gene:DOA4(YDR069C)|FD-Score:-3.33|P-value:4.33E-4||SGD DESC:Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole Gene:ECM2(YBR065C)|FD-Score:3.8|P-value:7.33E-5||SGD DESC:Pre-mRNA splicing factor, facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p Gene:EGT2(YNL327W)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Glycosylphosphatidylinositol (GPI)-anchored cell wall endoglucanase required for proper cell separation after cytokinesis, expression is activated by Swi5p and tightly regulated in a cell cycle-dependent manner Gene:FYV10(YIL097W)|FD-Score:3.94|P-value:4.14E-5||SGD DESC:Subunit of GID complex; involved in proteasome-dependent catabolite inactivation of gluconeogenic enzymes FBPase, PEPCK, and c-MDH; forms dimer with Rmd5p that is then recruited to GID Complex by Gid8p; contains a degenerate RING finger motif needed for GID complex ubiquitin ligase activity in vivo, as well as CTLH and CRA domains; plays role in anti-apoptosis; required for survival upon exposure to K1 killer toxin Gene:GLN3(YER040W)|FD-Score:3.38|P-value:3.66E-4||SGD DESC:Transcriptional activator of genes regulated by nitrogen catabolite repression (NCR), localization and activity regulated by quality of nitrogen source Gene:HMS1(YOR032C)|FD-Score:3.76|P-value:8.56E-5||SGD DESC:Basic helix-loop-helix (bHLH) protein with similarity to myc-family transcription factors; overexpression confers hyperfilamentous growth and suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant Gene:HOF1(YMR032W)|FD-Score:4.52|P-value:3.14E-6||SGD DESC:Bud neck-localized, SH3 domain-containing protein required for cytokinesis; is phosphorylated by Dbf2p; regulates actomyosin ring dynamics and septin localization; interacts with the formins, Bni1p and Bnr1p, and with Cyk3p, Vrp1p, and Bni5p Gene:HOT13(YKL084W)|FD-Score:-3.11|P-value:9.37E-4||SGD DESC:Zinc-binding mitochondrial intermembrane space (IMS) protein involved in a disulfide relay system for IMS import of cysteine-containing proteins; binds Mia40p and stimulates its Erv1p-dependent oxidation, probably by sequestering zinc Gene:ICE2(YIL090W)|FD-Score:-3.81|P-value:6.87E-5||SGD DESC:Integral ER membrane protein with type-III transmembrane domains; required for maintenance of ER zinc homeostasis; necessary for efficient targeting of Trm1p tRNA methyltransferase to inner nuclear membrane; mutations cause defects in cortical ER morphology in both the mother and daughter cells Gene:LSB6(YJL100W)|FD-Score:3.13|P-value:8.62E-4||SGD DESC:Type II phosphatidylinositol 4-kinase that binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization Gene:MCK1(YNL307C)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Protein serine/threonine/tyrosine (dual-specificity) kinase; involved in control of chromosome segregation and in regulating entry into meiosis; interacts with Clb2p and Mih1p; inhibits Clb2p-Cdk1 activity; related to mammalian glycogen synthase kinases of the GSK-3 family Gene:MCM21(YDR318W)|FD-Score:3.17|P-value:7.75E-4||SGD DESC:Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2 Gene:MSN2(YMR037C)|FD-Score:-3.45|P-value:2.83E-4||SGD DESC:Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN2 has a paralog, MSN4, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:NPP2(YEL016C)|FD-Score:5.03|P-value:2.46E-7||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp1p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP2 has a paralog, NPP1, that arose from the whole genome duplication Gene:OMA1(YKR087C)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Metalloendopeptidase of the mitochondrial inner membrane; involved in turnover of membrane-embedded proteins; mediates degradation of Cox1p in coa2 mutant cells; member of a family of predicted membrane-bound metallopeptidases in prokaryotes and higher eukaryotes Gene:OMS1(YDR316W)|FD-Score:3.21|P-value:6.68E-4||SGD DESC:Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations Gene:OSH2(YDL019C)|FD-Score:3.29|P-value:4.97E-4||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PDH1(YPR002W)|FD-Score:5.01|P-value:2.71E-7||SGD DESC:Mitochondrial protein that participates in respiration, induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate Gene:PFK2(YMR205C)|FD-Score:3.21|P-value:6.54E-4||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PHA2(YNL316C)|FD-Score:3.12|P-value:9.18E-4||SGD DESC:Prephenate dehydratase, catalyzes the conversion of prephanate to phenylpyruvate, which is a step in the phenylalanine biosynthesis pathway Gene:PTC4(YBR125C)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Cytoplasmic type 2C protein phosphatase (PP2C); identified as a high-copy number suppressor of cnb1 mpk1 synthetic lethality; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity Gene:PTP2(YOR208W)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the nucleus Gene:RAD6(YGL058W)|FD-Score:-3.56|P-value:1.88E-4||SGD DESC:Ubiquitin-conjugating enzyme (E2), involved in postreplication repair (as a heterodimer with Rad18p), DSBR and checkpoint control (as a heterodimer with Bre1p), ubiquitin-mediated N-end rule protein degradation (as a heterodimer with Ubr1p Gene:RPL26A(YLR344W)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Ribosomal 60S subunit protein L26A; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26A has a paralog, RPL26B, that arose from the whole genome duplication Gene:RPL42B(YHR141C)|FD-Score:-3.71|P-value:1.04E-4||SGD DESC:Ribosomal 60S subunit protein L42B; required for propagation of the killer toxin-encoding M1 double-stranded RNA satellite of the L-A double-stranded RNA virus; homologous to mammalian ribosomal protein L36A, no bacterial homolog; RPL42B has a paralog, RPL42A, that arose from the whole genome duplication Gene:RPL4A(YBR031W)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Ribosomal 60S subunit protein L4A; N-terminally acetylated; homologous to mammalian ribosomal protein L4 and bacterial L4; RPL4A has a paralog, RPL4B, that arose from the whole genome duplication Gene:RPS18B(YML026C)|FD-Score:4.78|P-value:8.95E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18B has a paralog, RPS18A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SET5(YHR207C)|FD-Score:-3.34|P-value:4.15E-4||SGD DESC:Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus Gene:SNQ2(YDR011W)|FD-Score:-3.12|P-value:9.11E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SPR6(YER115C)|FD-Score:3.26|P-value:5.65E-4||SGD DESC:Protein of unknown function, expressed during sporulation; not required for sporulation, but gene exhibits genetic interactions with other genes required for sporulation Gene:SPS18(YNL204C)|FD-Score:5.73|P-value:5.08E-9||SGD DESC:Protein of unknown function, contains a putative zinc-binding domain; expressed during sporulation Gene:TDA5(YLR426W_p)|FD-Score:-5.51|P-value:1.79E-8||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:TDH2(YJR009C)|FD-Score:-3.35|P-value:4.02E-4||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress; TDH2 has a paralog, TDH3, that arose from the whole genome duplication Gene:TES1(YJR019C)|FD-Score:3.98|P-value:3.42E-5||SGD DESC:Peroxisomal acyl-CoA thioesterase likely to be involved in fatty acid oxidation rather than fatty acid synthesis; conserved protein also found in human peroxisomes; TES1 mRNA levels increase during growth on fatty acids Gene:TGS1(YPL157W)|FD-Score:4.48|P-value:3.78E-6||SGD DESC:Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs Gene:TRP2(YER090W)|FD-Score:-3.48|P-value:2.48E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:-3.96|P-value:3.70E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TYW3(YGL050W)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:tRNA methyltransferase required for synthesis of wybutosine, a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions Gene:UGA2(YBR006W)|FD-Score:4.19|P-value:1.40E-5||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:URE2(YNL229C)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VAC17(YCL063W)|FD-Score:-3.98|P-value:3.40E-5||SGD DESC:Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p Gene:VPS55(YJR044C)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP) Gene:YAL018C(YAL018C_p)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Putative protein of unknown function Gene:YAP5(YIR018W)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Basic leucine zipper (bZIP) iron-sensing transcription factor; YAP5 has a paralog, YAP7, that arose from the whole genome duplication Gene:YCL049C(YCL049C_p)|FD-Score:3.61|P-value:1.50E-4||SGD DESC:Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene Gene:YDR271C(YDR271C_d)|FD-Score:-3.11|P-value:9.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W Gene:YDR444W(YDR444W_p)|FD-Score:5.25|P-value:7.45E-8||SGD DESC:Putative hydrolase acting on ester bonds Gene:YGR160W(YGR160W_d)|FD-Score:-4.12|P-value:1.86E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR273C(YGR273C_p)|FD-Score:4.11|P-value:1.98E-5||SGD DESC:Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene Gene:YGR290W(YGR290W_d)|FD-Score:5.26|P-value:7.23E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) Gene:YIM2(YMR151W_d)|FD-Score:4.43|P-value:4.74E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1 Gene:YMR209C(YMR209C_p)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; YMR209C is not an essential gene Gene:YNL122C(YNL122C_p)|FD-Score:3.76|P-value:8.50E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL122C is not an essential gene Gene:YPK1(YKL126W)|FD-Score:-3.58|P-value:1.74E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL039W(YPL039W_p)|FD-Score:3.15|P-value:8.15E-4||SGD DESC:Putative protein of unknown function; YPL039W is not an essential gene

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGR267C5.219.39E-80.61FOL2GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway
YMR146C4.631.84E-60.61TIF34eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation
YDL004W4.241.10E-50.61ATP16Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated
YER021W3.904.86E-50.61RPN3Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control
YBR004C3.295.05E-40.27GPI18Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria
YGR280C3.020.001280.05PXR1Essential protein involved in rRNA and snoRNA maturation; competes with TLC1 RNA for binding to Est2p, suggesting a role in negative regulation of telomerase; human homolog inhibits telomerase; contains a G-patch RNA interacting domain
YMR268C2.970.001500.07PRP24Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling
YBL105C2.900.001890.02PKC1Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
YNL244C2.880.001980.03SUI1Translation initiation factor eIF1; component of a complex involved in recognition of the initiator codon; modulates translation accuracy at the initiation phase
YKL035W2.850.002150.00UGP1UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication
YGR277C2.850.002180.02CAB4Probable pantetheine-phosphate adenylyltransferase (PPAT), which catalyzes the fourth step in the biosynthesis of coenzyme A from pantothenate; null mutant lethality is complemented by E. coli coaD (encoding PPAT); widely conserved
YMR211W2.830.002300.06DML1Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
YPL146C2.770.002800.10NOP53Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired
YMR239C2.670.003790.03RNT1Nuclear dsRNA-specific ribonuclease (RNase III); involved in rDNA transcription, rRNA processing and U2 snRNA 3' end formation by cleavage of a stem-loop structure at the 3' end of U2 snRNA; involved in polyadenylation-independent transcription termination; involved in the cell wall stress response, regulating the degradation of cell wall integrity and morphogenesis checkpoint genes
YJL069C2.640.004120.05UTP18Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YNL204C5.735.08E-9SPS18Protein of unknown function, contains a putative zinc-binding domain; expressed during sporulation
YGR290W_d5.267.23E-8YGR290W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter)
YDR444W_p5.257.45E-8YDR444W_pPutative hydrolase acting on ester bonds
YEL016C5.032.46E-7NPP2Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp1p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP2 has a paralog, NPP1, that arose from the whole genome duplication
YPR002W5.012.71E-7PDH1Mitochondrial protein that participates in respiration, induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate
YML026C4.788.95E-7RPS18BProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18B has a paralog, RPS18A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
YMR032W4.523.14E-6HOF1Bud neck-localized, SH3 domain-containing protein required for cytokinesis; is phosphorylated by Dbf2p; regulates actomyosin ring dynamics and septin localization; interacts with the formins, Bni1p and Bnr1p, and with Cyk3p, Vrp1p, and Bni5p
YPL157W4.483.78E-6TGS1Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs
YMR151W_d4.434.74E-6YIM2_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1
YAL020C4.347.02E-6ATS1Protein required, with Elongator complex, Kti11p, and Kti12p, for modification of wobble nucleosides in tRNA; has a potential role in regulatory interactions between microtubules and the cell cycle
YAL018C_p4.261.01E-5YAL018C_pPutative protein of unknown function
YBR006W4.191.40E-5UGA2Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm
YJL058C4.161.59E-5BIT61Subunit of TORC2 membrane-associated complex; involved in regulation of cell cycle-dependent actin cytoskeletal dynamics during polarized growth and cell wall integrity; BIT61 has a paralog, BIT2, that arose from the whole genome duplication
YGR273C_p4.111.98E-5YGR273C_pPutative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene
YJR019C3.983.42E-5TES1Peroxisomal acyl-CoA thioesterase likely to be involved in fatty acid oxidation rather than fatty acid synthesis; conserved protein also found in human peroxisomes; TES1 mRNA levels increase during growth on fatty acids

GO enrichment analysis for SGTC_2761
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0815.61E-10SGTC_30839117465 49.5 μMChembridge (Drug-like library)164209150.0666667
0.0773.78E-9SGTC_2779methotrexate 400.0 μMMiscellaneous1269410.0470588
0.0681.80E-7SGTC_30849116877 49.5 μMChembridge (Drug-like library)272413250.0985916
0.0647.11E-7SGTC_22567954252 200.0 μMChembridge (Fragment library)29729200.0877193mitochondrial processes
0.0595.55E-6SGTC_506n9-isopropylolomoucine 153.0 μMICCB bioactive library66103550.101449
0.0542.84E-5SGTC_2747doxorubicin 7.6 μMMiscellaneous317030.0625anthracycline transcription coupled DNA repair
0.0543.62E-5SGTC_31609100216 49.5 μMChembridge (Drug-like library)171910390.144928
0.0501.16E-4SGTC_1559coumarin 343 70.1 μMTimTec (Pure natural product library)1087700.0909091
0.0472.96E-4SGTC_1727st034309 9.9 μMTimTec (Natural product derivative library)8703050.0491803
0.0473.33E-4SGTC_1644st010134 20.9 μMTimTec (Natural product derivative library)38181340.0645161mitochondrial processes
0.0463.53E-4SGTC_28669034245 58.4 μMChembridge (Drug-like library)64622730.0746269
0.0463.63E-4SGTC_683panaxydol 45.0 μMMiscellaneous52832800.0149254sphingolipid biosynthesis & PDR1
0.0463.86E-4SGTC_28639028737 58.4 μMChembridge (Drug-like library)64609170.0714286
0.0455.33E-4SGTC_32969122258 64.0 μMChembridge (Drug-like library)47304740.0869565redox potentiating
0.0447.37E-4SGTC_9443370-0224 231.0 μMChemDiv (Drug-like library)45805140.0847458

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_14214029-024971.4 μM0.2407412894095ChemDiv (Drug-like library)283.3400233.71802
SGTC_2350902389538.85 μM0.2352942997209Chembridge (Fragment library)266.337622.29402ergosterol biosynthesis
SGTC_14754092-104811.7 μM0.2264152881759ChemDiv (Drug-like library)338.529465.02102plasma membrane duress
SGTC_20204017018200 μM0.22222221127491Chembridge (Fragment library)320.36530.23957
SGTC_10194262-02123.4 μM0.2181824677213ChemDiv (Drug-like library)352.4881635.20803ergosterol depletion effects on membrane
SGTC_11473809-104512.3 μM0.217391325925ChemDiv (Drug-like library)293.402723.42322
SGTC_20865274770176.62 μM0.217391689278Chembridge (Fragment library)254.31031.46325
SGTC_5144466-00382.65 nM0.2115385722064ChemDiv (Drug-like library)294.390783.83813cell wall
SGTC_9164466-003842 nM0.2115385722064ChemDiv (Drug-like library)294.390783.83813cell wall
SGTC_12054161-278576 μM0.2115383498106ChemDiv (Drug-like library)224.300943.64711
SGTC_3124092-06536.84 μM0.2105261148278ChemDiv (Drug-like library)366.4716834.54903ergosterol depletion effects on membrane