leflunomide FDA approved compound

5-methyl-N-[4-(trifluoromethyl)phenyl]-1,2-oxazole-4-carboxamide


Established Yeast Drug Target : URA1

A prodrug with immunosuppresive properties, leflunomide is converted to an active metabolite that inhibits dihydroorotate dehydrogenase, a key enzyme of de novo pyrimidine synthesis. This agent also inhibits various protein tyrosine kinases, such as protein kinase C (PKC).

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PubChem MeSH terms: Antirheumatic Agents;Enzyme Inhibitors;Immunosuppressive Agents



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2762
Screen concentration 42.1 μM
Source Miscellaneous
PubChem CID 3899
SMILES CC1=C(C=NO1)C(=O)NC2=CC=C(C=C2)C(F)(F)F
Standardized SMILES Cc1oncc1C(=O)Nc2ccc(cc2)C(F)(F)F
Molecular weight 270.2073
ALogP 2.16
H-bond donor count 1
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 11.76
% growth inhibition (Hom. pool) 7.66


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3899
Download HIP data (tab-delimited text)  (excel)
Gene:ALG14(YBR070C)|FD-Score:3.84|P-value:6.13E-5|Clearance:0||SGD DESC:Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases Gene:GPI11(YDR302W)|FD-Score:3.82|P-value:6.62E-5|Clearance:0.11||SGD DESC:ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog Gene:HIP1(YGR191W)|FD-Score:3.37|P-value:3.75E-4|Clearance:0.03||SGD DESC:High-affinity histidine permease, also involved in the transport of manganese ions Gene:HTB1(YDR224C)|FD-Score:3.13|P-value:8.80E-4|Clearance:0||SGD DESC:Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation Gene:NIC96(YFR002W)|FD-Score:4.86|P-value:5.94E-7|Clearance:0.84||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:PKC1(YBL105C)|FD-Score:5.9|P-value:1.85E-9|Clearance:1.04||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RPB8(YOR224C)|FD-Score:3.44|P-value:2.95E-4|Clearance:0.07||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RRN7(YJL025W)|FD-Score:-3.19|P-value:7.18E-4|Clearance:0||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:SEC31(YDL195W)|FD-Score:3.3|P-value:4.76E-4|Clearance:0.18||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SPC98(YNL126W)|FD-Score:3.72|P-value:1.02E-4|Clearance:0.28||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:SUI2(YJR007W)|FD-Score:3.34|P-value:4.20E-4|Clearance:0.04||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:YAE1(YJR067C)|FD-Score:3.13|P-value:8.85E-4|Clearance:0.1||SGD DESC:Protein that forms a complex with Lto1p and Rli1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species Gene:YMR290W-A(YMR290W-A_d)|FD-Score:3.84|P-value:6.23E-5|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:YPR177C(YPR177C_d)|FD-Score:4.02|P-value:2.92E-5|Clearance:0.18||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex Gene:ALG14(YBR070C)|FD-Score:3.84|P-value:6.13E-5|Clearance:0||SGD DESC:Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases Gene:GPI11(YDR302W)|FD-Score:3.82|P-value:6.62E-5|Clearance:0.11||SGD DESC:ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog Gene:HIP1(YGR191W)|FD-Score:3.37|P-value:3.75E-4|Clearance:0.03||SGD DESC:High-affinity histidine permease, also involved in the transport of manganese ions Gene:HTB1(YDR224C)|FD-Score:3.13|P-value:8.80E-4|Clearance:0||SGD DESC:Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation Gene:NIC96(YFR002W)|FD-Score:4.86|P-value:5.94E-7|Clearance:0.84||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:PKC1(YBL105C)|FD-Score:5.9|P-value:1.85E-9|Clearance:1.04||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RPB8(YOR224C)|FD-Score:3.44|P-value:2.95E-4|Clearance:0.07||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RRN7(YJL025W)|FD-Score:-3.19|P-value:7.18E-4|Clearance:0||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:SEC31(YDL195W)|FD-Score:3.3|P-value:4.76E-4|Clearance:0.18||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SPC98(YNL126W)|FD-Score:3.72|P-value:1.02E-4|Clearance:0.28||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:SUI2(YJR007W)|FD-Score:3.34|P-value:4.20E-4|Clearance:0.04||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:YAE1(YJR067C)|FD-Score:3.13|P-value:8.85E-4|Clearance:0.1||SGD DESC:Protein that forms a complex with Lto1p and Rli1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species Gene:YMR290W-A(YMR290W-A_d)|FD-Score:3.84|P-value:6.23E-5|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:YPR177C(YPR177C_d)|FD-Score:4.02|P-value:2.92E-5|Clearance:0.18||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3899
Download HOP data (tab-delimited text)  (excel)
Gene:ATP23(YNR020C)|FD-Score:3.21|P-value:6.58E-4||SGD DESC:Putative metalloprotease of the mitochondrial inner membrane, required for processing of Atp6p; has an additional role in assembly of the F0 sector of the F1F0 ATP synthase complex; substrate of the Mia40p-Erv1p disulfide relay system, and folding is assisted by Mia40p Gene:BRP1(YGL007W_d)|FD-Score:4.37|P-value:6.35E-6||SGD DESC:Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Gene:CHO2(YGR157W)|FD-Score:4.71|P-value:1.26E-6||SGD DESC:Phosphatidylethanolamine methyltransferase (PEMT), catalyzes the first step in the conversion of phosphatidylethanolamine to phosphatidylcholine during the methylation pathway of phosphatidylcholine biosynthesis Gene:CLD1(YGR110W)|FD-Score:4.36|P-value:6.52E-6||SGD DESC:Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58 Gene:COG7(YGL005C)|FD-Score:3.79|P-value:7.66E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COQ1(YBR003W)|FD-Score:3.26|P-value:5.59E-4||SGD DESC:Hexaprenyl pyrophosphate synthetase, catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis Gene:EBS1(YDR206W)|FD-Score:3.84|P-value:6.26E-5||SGD DESC:Protein involved in inhibition of translation and nonsense-mediated decay; interacts with cap binding protein Cdc33p and with Nam7p; localizes to P-bodies upon glucose starvation; mRNA abundance regulated by mRNA decay factors Gene:EDC3(YEL015W)|FD-Score:-3.61|P-value:1.51E-4||SGD DESC:Non-essential conserved protein with a role in mRNA decapping; specifically affects the function of the decapping enzyme Dcp1p; localizes to cytoplasmic mRNA processing bodies; forms cytoplasmic foci upon DNA replication stress Gene:ELG1(YOR144C)|FD-Score:-3.42|P-value:3.19E-4||SGD DESC:Subunit of an alternative replication factor C complex important for DNA replication and genome integrity; suppresses spontaneous DNA damage; involved in homologous recombination-mediated repair and telomere homeostasis; required for PCNA (Pol30p) unloading during DNA replication Gene:EMC4(YGL231C)|FD-Score:3.96|P-value:3.73E-5||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4, fly CG11137, human TMM85 Gene:ERP6(YGL002W)|FD-Score:4.45|P-value:4.35E-6||SGD DESC:Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; ERP6 has a paralog, ERP1, that arose from the whole genome duplication Gene:GTO3(YMR251W)|FD-Score:5.63|P-value:8.88E-9||SGD DESC:Omega class glutathione transferase; putative cytosolic localization Gene:HAP3(YBL021C)|FD-Score:3.21|P-value:6.59E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences contributing to both complex assembly and DNA binding Gene:HRB1(YNL004W)|FD-Score:-3.14|P-value:8.55E-4||SGD DESC:Poly(A+) RNA-binding protein; involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Npl3p; HRB1 has a paralog, GBP2, that arose from the whole genome duplication Gene:ICE2(YIL090W)|FD-Score:5.82|P-value:2.90E-9||SGD DESC:Integral ER membrane protein with type-III transmembrane domains; required for maintenance of ER zinc homeostasis; necessary for efficient targeting of Trm1p tRNA methyltransferase to inner nuclear membrane; mutations cause defects in cortical ER morphology in both the mother and daughter cells Gene:ITR2(YOL103W)|FD-Score:3.87|P-value:5.55E-5||SGD DESC:Myo-inositol transporter with strong similarity to the major myo-inositol transporter Itr1p, member of the sugar transporter superfamily; expressed constitutively Gene:JHD1(YER051W)|FD-Score:-5|P-value:2.86E-7||SGD DESC:JmjC domain family histone demethylase specific for H3-K36, similar to proteins found in human, mouse, drosophila, X. laevis, C. elegans, and S. pombe Gene:JJJ2(YJL162C)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Protein of unknown function, contains a J-domain, which is a region with homology to the E. coli DnaJ protein Gene:KDX1(YKL161C)|FD-Score:5.69|P-value:6.38E-9||SGD DESC:Protein kinase implicated in the Slt2p mitogen-activated (MAP) kinase signaling pathway; interacts with numerous components in the mating pheromone and CWI MAPK pathways; associates with Rlm1p Gene:KTI11(YBL071W-A)|FD-Score:4.03|P-value:2.84E-5||SGD DESC:Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs Gene:LOS1(YKL205W)|FD-Score:3.89|P-value:5.10E-5||SGD DESC:Nuclear pore protein involved in nuclear export of pre-tRNA and in re-export of mature tRNAs after their retrograde import from the cytoplasm Gene:LTE1(YAL024C)|FD-Score:6.03|P-value:8.05E-10||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MAP1(YLR244C)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p Gene:MDL1(YLR188W)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, mediates export of peptides generated upon proteolysis of mitochondrial proteins, plays a role in the regulation of cellular resistance to oxidative stress Gene:MET13(YGL125W)|FD-Score:4.24|P-value:1.11E-5||SGD DESC:Major isozyme of methylenetetrahydrofolate reductase, catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the methionine biosynthesis pathway Gene:MET16(YPR167C)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:3'-phosphoadenylsulfate reductase, reduces 3'-phosphoadenylyl sulfate to adenosine-3',5'-bisphosphate and free sulfite using reduced thioredoxin as cosubstrate, involved in sulfate assimilation and methionine metabolism Gene:MIP6(YHR015W)|FD-Score:-3.34|P-value:4.12E-4||SGD DESC:Putative RNA-binding protein; interacts with Mex67p, which is a component of the nuclear pore involved in nuclear mRNA export; MIP6 has a paralog, PES4, that arose from the whole genome duplication Gene:MSS11(YMR164C)|FD-Score:-3.18|P-value:7.39E-4||SGD DESC:Transcription factor involved in regulation of invasive growth and starch degradation; controls the activation of FLO11 and STA2 in response to nutritional signals Gene:MVP1(YMR004W)|FD-Score:3.79|P-value:7.61E-5||SGD DESC:Protein required for sorting proteins to the vacuole; Mvp1p and Vps1p act in concert to promote membrane traffic to the vacuole; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions; protein abundance increases in response to DNA replication stress Gene:NAM7(YMR080C)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance; forms cytoplasmic foci upon DNA replication stress Gene:NTO1(YPR031W)|FD-Score:3.11|P-value:9.39E-4||SGD DESC:Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3 Gene:OAF3(YKR064W)|FD-Score:3.22|P-value:6.41E-4||SGD DESC:Putative transcriptional repressor with Zn(2)-Cys(6) finger; negatively regulates transcription in response to oleate levels, based on mutant phenotype and localization to oleate-responsive promoters; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:PAF1(YBR279W)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 Gene:PEX13(YLR191W)|FD-Score:3.9|P-value:4.90E-5||SGD DESC:Integral peroxisomal membrane protein required for translocation of peroxisomal matrix proteins, interacts with the PTS1 signal recognition factor Pex5p and the PTS2 signal recognition factor Pex7p, forms a complex with Pex14p and Pex17p Gene:POT1(YIL160C)|FD-Score:-3.55|P-value:1.91E-4||SGD DESC:3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids Gene:PPN1(YDR452W)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:PTH2(YBL057C)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth Gene:PUS7(YOR243C)|FD-Score:3.99|P-value:3.34E-5||SGD DESC:Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria Gene:RBK1(YCR036W)|FD-Score:5.7|P-value:6.16E-9||SGD DESC:Putative ribokinase Gene:RKM2(YDR198C)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:Ribosomal protein lysine methyltransferase, responsible for trimethylation of the lysine residue at position 3 of Rpl12Ap and Rpl12Bp Gene:RPN14(YGL004C)|FD-Score:3.87|P-value:5.55E-5||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasome regulatory particle (RP); null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; interacts with Rpt5p Gene:RPS18A(YDR450W)|FD-Score:3.1|P-value:9.80E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18A has a paralog, RPS18B, that arose from the whole genome duplication; protein increases in abundance and relocalizes from cytoplasm to nuclear foci upon DNA replication stress Gene:RPS28B(YLR264W)|FD-Score:3.19|P-value:7.23E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S28, no bacterial homolog; RPS28B has a paralog, RPS28A, that arose from the whole genome duplication Gene:RSF1(YMR030W)|FD-Score:4.19|P-value:1.39E-5||SGD DESC:Protein required for respiratory growth; localized to both the nucleus and mitochondrion; may interact with transcription factors to mediate the transition to respiratory growth and activate transcription of nuclear and mitochondrial genes Gene:SAN1(YDR143C)|FD-Score:3.98|P-value:3.47E-5||SGD DESC:Ubiquitin-protein ligase; involved in the proteasome-dependent degradation of aberrant nuclear proteins; targets substrates with regions of exposed hydrophobicity containing 5 or more contiguous hydrophobic residues; contains intrinsically disordered regions that contribute to substrate recognition Gene:SKY1(YMR216C)|FD-Score:3.18|P-value:7.31E-4||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SLX5(YDL013W)|FD-Score:4.45|P-value:4.32E-6||SGD DESC:Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex, stimulated by SUMO-modified substrates; contains a RING domain and two SIMs (SUMO-interacting motifs); forms SUMO-dependent nuclear foci, including DNA repair centers Gene:SNA4(YDL123W)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:Protein of unknown function, localized to the vacuolar outer membrane; predicted to be palmitoylated Gene:SNQ2(YDR011W)|FD-Score:-3.55|P-value:1.91E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SNU66(YOR308C)|FD-Score:3.72|P-value:9.93E-5||SGD DESC:Component of the U4/U6.U5 snRNP complex involved in pre-mRNA splicing via spliceosome; also required for pre-5S rRNA processing and may act in concert with Rnh70p; has homology to human SART-1 Gene:SPE2(YOL052C)|FD-Score:4.25|P-value:1.05E-5||SGD DESC:S-adenosylmethionine decarboxylase, required for the biosynthesis of spermidine and spermine; cells lacking Spe2p require spermine or spermidine for growth in the presence of oxygen but not when grown anaerobically Gene:SPS18(YNL204C)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Protein of unknown function, contains a putative zinc-binding domain; expressed during sporulation Gene:SSU1(YPL092W)|FD-Score:4.01|P-value:3.07E-5||SGD DESC:Plasma membrane sulfite pump involved in sulfite metabolism and required for efficient sulfite efflux; major facilitator superfamily protein Gene:STB5(YHR178W)|FD-Score:3.77|P-value:8.11E-5||SGD DESC:Transcription factor, involved in regulating multidrug resistance and oxidative stress response; forms a heterodimer with Pdr1p; contains a Zn(II)2Cys6 zinc finger domain that interacts with a pleiotropic drug resistance element in vitro Gene:SWC7(YLR385C)|FD-Score:4.03|P-value:2.76E-5||SGD DESC:Protein of unknown function, component of the Swr1p complex that incorporates Htz1p into chromatin Gene:TAT1(YBR069C)|FD-Score:3.89|P-value:5.11E-5||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TAZ1(YPR140W)|FD-Score:5.15|P-value:1.30E-7||SGD DESC:Lyso-phosphatidylcholine acyltransferase, required for normal phospholipid content of mitochondrial membranes; may remodel acyl groups of cardiolipin in the inner membrane; human ortholog tafazzin is implicated in Barth syndrome Gene:THI7(YLR237W)|FD-Score:4.81|P-value:7.68E-7||SGD DESC:Plasma membrane transporter responsible for the uptake of thiamine, member of the major facilitator superfamily of transporters; mutation of human ortholog causes thiamine-responsive megaloblastic anemia Gene:TMS1(YDR105C)|FD-Score:4.48|P-value:3.81E-6||SGD DESC:Vacuolar membrane protein of unknown function that is conserved in mammals; predicted to contain eleven transmembrane helices; interacts with Pdr5p, a protein involved in multidrug resistance Gene:TPC1(YGR096W)|FD-Score:5.07|P-value:2.02E-7||SGD DESC:Mitochondrial membrane transporter that mediates uptake of the essential cofactor thiamine pyrophosphate (ThPP) into mitochondria; expression appears to be regulated by carbon source; member of the mitochondrial carrier family Gene:TRM82(YDR165W)|FD-Score:5.25|P-value:7.75E-8||SGD DESC:Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p Gene:TUL1(YKL034W)|FD-Score:-3.49|P-value:2.37E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1 Gene:TVP15(YDR100W)|FD-Score:3.84|P-value:6.21E-5||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p Gene:UBX7(YBR273C)|FD-Score:-3.14|P-value:8.44E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p; UBX7 has a paralog, UBX6, that arose from the whole genome duplication Gene:UTR2(YEL040W)|FD-Score:4.22|P-value:1.21E-5||SGD DESC:Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar to and functionally redundant with Crh1; glycosylphosphatidylinositol (GPI)-anchored protein localized to bud neck Gene:YBL008W-A(YBL008W-A_p)|FD-Score:-3.17|P-value:7.66E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDL071C(YDL071C_d)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF BDF2/YDL070W Gene:YDR401W(YDR401W_d)|FD-Score:3.15|P-value:8.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR417C(YDR417C_d)|FD-Score:3.21|P-value:6.67E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RPL12B/YDR418W Gene:YDR442W(YDR442W_d)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL177W(YGL177W_d)|FD-Score:4.59|P-value:2.21E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL242C(YGL242C_p)|FD-Score:6.05|P-value:7.21E-10||SGD DESC:Putative protein of unknown function; deletion mutant is viable Gene:YIL108W(YIL108W_p)|FD-Score:-6.07|P-value:6.21E-10||SGD DESC:Putative metalloendopeptidase; forms cytoplasmic foci upon DNA replication stress Gene:YJL047C-A(YJL047C-A_p)|FD-Score:-4.64|P-value:1.78E-6||SGD DESC:Putative protein of unknown function Gene:YJL163C(YJL163C_p)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Putative protein of unknown function Gene:YJR038C(YJR038C_d)|FD-Score:-4.29|P-value:9.07E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR342W-A(YLR342W-A_p)|FD-Score:-3.42|P-value:3.14E-4||SGD DESC:Putative protein of unknown function Gene:YMC1(YPR058W)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Putative mitochondrial inner membrane transporter; proposed role in oleate metabolism and glutamate biosynthesis; member of the mitochondrial carrier (MCF) family; YMC1 has a paralog, YMC2, that arose from the whole genome duplication Gene:YME2(YMR302C)|FD-Score:-3.7|P-value:1.06E-4||SGD DESC:Integral inner mitochondrial membrane protein with a role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases Gene:YMR034C(YMR034C_p)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Putative transporter, member of the SLC10 carrier family; identified in a transposon mutagenesis screen as a gene involved in azole resistance; YMR034C is not an essential gene Gene:YMR173W-A(YMR173W-A_d)|FD-Score:3.93|P-value:4.23E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W Gene:YNL097C-B(YNL097C-B_p)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Putative protein of unknown function Gene:YOL097W-A(YOL097W-A_p)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YOR200W(YOR200W_d)|FD-Score:5.96|P-value:1.23E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c Gene:YPT53(YNL093W)|FD-Score:3.82|P-value:6.75E-5||SGD DESC:Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication Gene:ATP23(YNR020C)|FD-Score:3.21|P-value:6.58E-4||SGD DESC:Putative metalloprotease of the mitochondrial inner membrane, required for processing of Atp6p; has an additional role in assembly of the F0 sector of the F1F0 ATP synthase complex; substrate of the Mia40p-Erv1p disulfide relay system, and folding is assisted by Mia40p Gene:BRP1(YGL007W_d)|FD-Score:4.37|P-value:6.35E-6||SGD DESC:Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Gene:CHO2(YGR157W)|FD-Score:4.71|P-value:1.26E-6||SGD DESC:Phosphatidylethanolamine methyltransferase (PEMT), catalyzes the first step in the conversion of phosphatidylethanolamine to phosphatidylcholine during the methylation pathway of phosphatidylcholine biosynthesis Gene:CLD1(YGR110W)|FD-Score:4.36|P-value:6.52E-6||SGD DESC:Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58 Gene:COG7(YGL005C)|FD-Score:3.79|P-value:7.66E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COQ1(YBR003W)|FD-Score:3.26|P-value:5.59E-4||SGD DESC:Hexaprenyl pyrophosphate synthetase, catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis Gene:EBS1(YDR206W)|FD-Score:3.84|P-value:6.26E-5||SGD DESC:Protein involved in inhibition of translation and nonsense-mediated decay; interacts with cap binding protein Cdc33p and with Nam7p; localizes to P-bodies upon glucose starvation; mRNA abundance regulated by mRNA decay factors Gene:EDC3(YEL015W)|FD-Score:-3.61|P-value:1.51E-4||SGD DESC:Non-essential conserved protein with a role in mRNA decapping; specifically affects the function of the decapping enzyme Dcp1p; localizes to cytoplasmic mRNA processing bodies; forms cytoplasmic foci upon DNA replication stress Gene:ELG1(YOR144C)|FD-Score:-3.42|P-value:3.19E-4||SGD DESC:Subunit of an alternative replication factor C complex important for DNA replication and genome integrity; suppresses spontaneous DNA damage; involved in homologous recombination-mediated repair and telomere homeostasis; required for PCNA (Pol30p) unloading during DNA replication Gene:EMC4(YGL231C)|FD-Score:3.96|P-value:3.73E-5||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4, fly CG11137, human TMM85 Gene:ERP6(YGL002W)|FD-Score:4.45|P-value:4.35E-6||SGD DESC:Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; ERP6 has a paralog, ERP1, that arose from the whole genome duplication Gene:GTO3(YMR251W)|FD-Score:5.63|P-value:8.88E-9||SGD DESC:Omega class glutathione transferase; putative cytosolic localization Gene:HAP3(YBL021C)|FD-Score:3.21|P-value:6.59E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences contributing to both complex assembly and DNA binding Gene:HRB1(YNL004W)|FD-Score:-3.14|P-value:8.55E-4||SGD DESC:Poly(A+) RNA-binding protein; involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Npl3p; HRB1 has a paralog, GBP2, that arose from the whole genome duplication Gene:ICE2(YIL090W)|FD-Score:5.82|P-value:2.90E-9||SGD DESC:Integral ER membrane protein with type-III transmembrane domains; required for maintenance of ER zinc homeostasis; necessary for efficient targeting of Trm1p tRNA methyltransferase to inner nuclear membrane; mutations cause defects in cortical ER morphology in both the mother and daughter cells Gene:ITR2(YOL103W)|FD-Score:3.87|P-value:5.55E-5||SGD DESC:Myo-inositol transporter with strong similarity to the major myo-inositol transporter Itr1p, member of the sugar transporter superfamily; expressed constitutively Gene:JHD1(YER051W)|FD-Score:-5|P-value:2.86E-7||SGD DESC:JmjC domain family histone demethylase specific for H3-K36, similar to proteins found in human, mouse, drosophila, X. laevis, C. elegans, and S. pombe Gene:JJJ2(YJL162C)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Protein of unknown function, contains a J-domain, which is a region with homology to the E. coli DnaJ protein Gene:KDX1(YKL161C)|FD-Score:5.69|P-value:6.38E-9||SGD DESC:Protein kinase implicated in the Slt2p mitogen-activated (MAP) kinase signaling pathway; interacts with numerous components in the mating pheromone and CWI MAPK pathways; associates with Rlm1p Gene:KTI11(YBL071W-A)|FD-Score:4.03|P-value:2.84E-5||SGD DESC:Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs Gene:LOS1(YKL205W)|FD-Score:3.89|P-value:5.10E-5||SGD DESC:Nuclear pore protein involved in nuclear export of pre-tRNA and in re-export of mature tRNAs after their retrograde import from the cytoplasm Gene:LTE1(YAL024C)|FD-Score:6.03|P-value:8.05E-10||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MAP1(YLR244C)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p Gene:MDL1(YLR188W)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, mediates export of peptides generated upon proteolysis of mitochondrial proteins, plays a role in the regulation of cellular resistance to oxidative stress Gene:MET13(YGL125W)|FD-Score:4.24|P-value:1.11E-5||SGD DESC:Major isozyme of methylenetetrahydrofolate reductase, catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the methionine biosynthesis pathway Gene:MET16(YPR167C)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:3'-phosphoadenylsulfate reductase, reduces 3'-phosphoadenylyl sulfate to adenosine-3',5'-bisphosphate and free sulfite using reduced thioredoxin as cosubstrate, involved in sulfate assimilation and methionine metabolism Gene:MIP6(YHR015W)|FD-Score:-3.34|P-value:4.12E-4||SGD DESC:Putative RNA-binding protein; interacts with Mex67p, which is a component of the nuclear pore involved in nuclear mRNA export; MIP6 has a paralog, PES4, that arose from the whole genome duplication Gene:MSS11(YMR164C)|FD-Score:-3.18|P-value:7.39E-4||SGD DESC:Transcription factor involved in regulation of invasive growth and starch degradation; controls the activation of FLO11 and STA2 in response to nutritional signals Gene:MVP1(YMR004W)|FD-Score:3.79|P-value:7.61E-5||SGD DESC:Protein required for sorting proteins to the vacuole; Mvp1p and Vps1p act in concert to promote membrane traffic to the vacuole; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions; protein abundance increases in response to DNA replication stress Gene:NAM7(YMR080C)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance; forms cytoplasmic foci upon DNA replication stress Gene:NTO1(YPR031W)|FD-Score:3.11|P-value:9.39E-4||SGD DESC:Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3 Gene:OAF3(YKR064W)|FD-Score:3.22|P-value:6.41E-4||SGD DESC:Putative transcriptional repressor with Zn(2)-Cys(6) finger; negatively regulates transcription in response to oleate levels, based on mutant phenotype and localization to oleate-responsive promoters; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:PAF1(YBR279W)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 Gene:PEX13(YLR191W)|FD-Score:3.9|P-value:4.90E-5||SGD DESC:Integral peroxisomal membrane protein required for translocation of peroxisomal matrix proteins, interacts with the PTS1 signal recognition factor Pex5p and the PTS2 signal recognition factor Pex7p, forms a complex with Pex14p and Pex17p Gene:POT1(YIL160C)|FD-Score:-3.55|P-value:1.91E-4||SGD DESC:3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids Gene:PPN1(YDR452W)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:PTH2(YBL057C)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth Gene:PUS7(YOR243C)|FD-Score:3.99|P-value:3.34E-5||SGD DESC:Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria Gene:RBK1(YCR036W)|FD-Score:5.7|P-value:6.16E-9||SGD DESC:Putative ribokinase Gene:RKM2(YDR198C)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:Ribosomal protein lysine methyltransferase, responsible for trimethylation of the lysine residue at position 3 of Rpl12Ap and Rpl12Bp Gene:RPN14(YGL004C)|FD-Score:3.87|P-value:5.55E-5||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasome regulatory particle (RP); null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; interacts with Rpt5p Gene:RPS18A(YDR450W)|FD-Score:3.1|P-value:9.80E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18A has a paralog, RPS18B, that arose from the whole genome duplication; protein increases in abundance and relocalizes from cytoplasm to nuclear foci upon DNA replication stress Gene:RPS28B(YLR264W)|FD-Score:3.19|P-value:7.23E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S28, no bacterial homolog; RPS28B has a paralog, RPS28A, that arose from the whole genome duplication Gene:RSF1(YMR030W)|FD-Score:4.19|P-value:1.39E-5||SGD DESC:Protein required for respiratory growth; localized to both the nucleus and mitochondrion; may interact with transcription factors to mediate the transition to respiratory growth and activate transcription of nuclear and mitochondrial genes Gene:SAN1(YDR143C)|FD-Score:3.98|P-value:3.47E-5||SGD DESC:Ubiquitin-protein ligase; involved in the proteasome-dependent degradation of aberrant nuclear proteins; targets substrates with regions of exposed hydrophobicity containing 5 or more contiguous hydrophobic residues; contains intrinsically disordered regions that contribute to substrate recognition Gene:SKY1(YMR216C)|FD-Score:3.18|P-value:7.31E-4||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SLX5(YDL013W)|FD-Score:4.45|P-value:4.32E-6||SGD DESC:Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex, stimulated by SUMO-modified substrates; contains a RING domain and two SIMs (SUMO-interacting motifs); forms SUMO-dependent nuclear foci, including DNA repair centers Gene:SNA4(YDL123W)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:Protein of unknown function, localized to the vacuolar outer membrane; predicted to be palmitoylated Gene:SNQ2(YDR011W)|FD-Score:-3.55|P-value:1.91E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SNU66(YOR308C)|FD-Score:3.72|P-value:9.93E-5||SGD DESC:Component of the U4/U6.U5 snRNP complex involved in pre-mRNA splicing via spliceosome; also required for pre-5S rRNA processing and may act in concert with Rnh70p; has homology to human SART-1 Gene:SPE2(YOL052C)|FD-Score:4.25|P-value:1.05E-5||SGD DESC:S-adenosylmethionine decarboxylase, required for the biosynthesis of spermidine and spermine; cells lacking Spe2p require spermine or spermidine for growth in the presence of oxygen but not when grown anaerobically Gene:SPS18(YNL204C)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Protein of unknown function, contains a putative zinc-binding domain; expressed during sporulation Gene:SSU1(YPL092W)|FD-Score:4.01|P-value:3.07E-5||SGD DESC:Plasma membrane sulfite pump involved in sulfite metabolism and required for efficient sulfite efflux; major facilitator superfamily protein Gene:STB5(YHR178W)|FD-Score:3.77|P-value:8.11E-5||SGD DESC:Transcription factor, involved in regulating multidrug resistance and oxidative stress response; forms a heterodimer with Pdr1p; contains a Zn(II)2Cys6 zinc finger domain that interacts with a pleiotropic drug resistance element in vitro Gene:SWC7(YLR385C)|FD-Score:4.03|P-value:2.76E-5||SGD DESC:Protein of unknown function, component of the Swr1p complex that incorporates Htz1p into chromatin Gene:TAT1(YBR069C)|FD-Score:3.89|P-value:5.11E-5||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TAZ1(YPR140W)|FD-Score:5.15|P-value:1.30E-7||SGD DESC:Lyso-phosphatidylcholine acyltransferase, required for normal phospholipid content of mitochondrial membranes; may remodel acyl groups of cardiolipin in the inner membrane; human ortholog tafazzin is implicated in Barth syndrome Gene:THI7(YLR237W)|FD-Score:4.81|P-value:7.68E-7||SGD DESC:Plasma membrane transporter responsible for the uptake of thiamine, member of the major facilitator superfamily of transporters; mutation of human ortholog causes thiamine-responsive megaloblastic anemia Gene:TMS1(YDR105C)|FD-Score:4.48|P-value:3.81E-6||SGD DESC:Vacuolar membrane protein of unknown function that is conserved in mammals; predicted to contain eleven transmembrane helices; interacts with Pdr5p, a protein involved in multidrug resistance Gene:TPC1(YGR096W)|FD-Score:5.07|P-value:2.02E-7||SGD DESC:Mitochondrial membrane transporter that mediates uptake of the essential cofactor thiamine pyrophosphate (ThPP) into mitochondria; expression appears to be regulated by carbon source; member of the mitochondrial carrier family Gene:TRM82(YDR165W)|FD-Score:5.25|P-value:7.75E-8||SGD DESC:Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p Gene:TUL1(YKL034W)|FD-Score:-3.49|P-value:2.37E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1 Gene:TVP15(YDR100W)|FD-Score:3.84|P-value:6.21E-5||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p Gene:UBX7(YBR273C)|FD-Score:-3.14|P-value:8.44E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p; UBX7 has a paralog, UBX6, that arose from the whole genome duplication Gene:UTR2(YEL040W)|FD-Score:4.22|P-value:1.21E-5||SGD DESC:Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar to and functionally redundant with Crh1; glycosylphosphatidylinositol (GPI)-anchored protein localized to bud neck Gene:YBL008W-A(YBL008W-A_p)|FD-Score:-3.17|P-value:7.66E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDL071C(YDL071C_d)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF BDF2/YDL070W Gene:YDR401W(YDR401W_d)|FD-Score:3.15|P-value:8.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR417C(YDR417C_d)|FD-Score:3.21|P-value:6.67E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RPL12B/YDR418W Gene:YDR442W(YDR442W_d)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL177W(YGL177W_d)|FD-Score:4.59|P-value:2.21E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL242C(YGL242C_p)|FD-Score:6.05|P-value:7.21E-10||SGD DESC:Putative protein of unknown function; deletion mutant is viable Gene:YIL108W(YIL108W_p)|FD-Score:-6.07|P-value:6.21E-10||SGD DESC:Putative metalloendopeptidase; forms cytoplasmic foci upon DNA replication stress Gene:YJL047C-A(YJL047C-A_p)|FD-Score:-4.64|P-value:1.78E-6||SGD DESC:Putative protein of unknown function Gene:YJL163C(YJL163C_p)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Putative protein of unknown function Gene:YJR038C(YJR038C_d)|FD-Score:-4.29|P-value:9.07E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR342W-A(YLR342W-A_p)|FD-Score:-3.42|P-value:3.14E-4||SGD DESC:Putative protein of unknown function Gene:YMC1(YPR058W)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Putative mitochondrial inner membrane transporter; proposed role in oleate metabolism and glutamate biosynthesis; member of the mitochondrial carrier (MCF) family; YMC1 has a paralog, YMC2, that arose from the whole genome duplication Gene:YME2(YMR302C)|FD-Score:-3.7|P-value:1.06E-4||SGD DESC:Integral inner mitochondrial membrane protein with a role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases Gene:YMR034C(YMR034C_p)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Putative transporter, member of the SLC10 carrier family; identified in a transposon mutagenesis screen as a gene involved in azole resistance; YMR034C is not an essential gene Gene:YMR173W-A(YMR173W-A_d)|FD-Score:3.93|P-value:4.23E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W Gene:YNL097C-B(YNL097C-B_p)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Putative protein of unknown function Gene:YOL097W-A(YOL097W-A_p)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YOR200W(YOR200W_d)|FD-Score:5.96|P-value:1.23E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c Gene:YPT53(YNL093W)|FD-Score:3.82|P-value:6.75E-5||SGD DESC:Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBL105C5.901.85E-91.04PKC1Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
YFR002W4.865.94E-70.84NIC96Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p)
YPR177C_d4.022.92E-50.18YPR177C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex
YBR070C3.846.13E-50.00ALG14Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases
YMR290W-A_d3.846.23E-50.01YMR290W-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase
YDR302W3.826.62E-50.11GPI11ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog
YNL126W3.721.02E-40.28SPC98Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
YOR224C3.442.95E-40.07RPB8RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III
YGR191W3.373.75E-40.03HIP1High-affinity histidine permease, also involved in the transport of manganese ions
YJR007W3.344.20E-40.04SUI2Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress
YDL195W3.304.76E-40.18SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YDR224C3.138.80E-40.00HTB1Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation
YJR067C3.138.85E-40.10YAE1Protein that forms a complex with Lto1p and Rli1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species
YKR071C3.030.001240.01DRE2Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress
YKL024C3.010.001290.01URA6Uridylate kinase, catalyzes the seventh enzymatic step in the de novo biosynthesis of pyrimidines, converting uridine monophosphate (UMP) into uridine-5'-diphosphate (UDP)

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL242C_p6.057.21E-10YGL242C_pPutative protein of unknown function; deletion mutant is viable
YAL024C6.038.05E-10LTE1Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures
YOR200W_d5.961.23E-9YOR200W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c
YIL090W5.822.90E-9ICE2Integral ER membrane protein with type-III transmembrane domains; required for maintenance of ER zinc homeostasis; necessary for efficient targeting of Trm1p tRNA methyltransferase to inner nuclear membrane; mutations cause defects in cortical ER morphology in both the mother and daughter cells
YCR036W5.706.16E-9RBK1Putative ribokinase
YKL161C5.696.38E-9KDX1Protein kinase implicated in the Slt2p mitogen-activated (MAP) kinase signaling pathway; interacts with numerous components in the mating pheromone and CWI MAPK pathways; associates with Rlm1p
YMR251W5.638.88E-9GTO3Omega class glutathione transferase; putative cytosolic localization
YDR165W5.257.75E-8TRM82Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p
YPR140W5.151.30E-7TAZ1Lyso-phosphatidylcholine acyltransferase, required for normal phospholipid content of mitochondrial membranes; may remodel acyl groups of cardiolipin in the inner membrane; human ortholog tafazzin is implicated in Barth syndrome
YGR096W5.072.02E-7TPC1Mitochondrial membrane transporter that mediates uptake of the essential cofactor thiamine pyrophosphate (ThPP) into mitochondria; expression appears to be regulated by carbon source; member of the mitochondrial carrier family
YLR237W4.817.68E-7THI7Plasma membrane transporter responsible for the uptake of thiamine, member of the major facilitator superfamily of transporters; mutation of human ortholog causes thiamine-responsive megaloblastic anemia
YGR157W4.711.26E-6CHO2Phosphatidylethanolamine methyltransferase (PEMT), catalyzes the first step in the conversion of phosphatidylethanolamine to phosphatidylcholine during the methylation pathway of phosphatidylcholine biosynthesis
YGL177W_d4.592.21E-6YGL177W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YDR105C4.483.81E-6TMS1Vacuolar membrane protein of unknown function that is conserved in mammals; predicted to contain eleven transmembrane helices; interacts with Pdr5p, a protein involved in multidrug resistance
YDL013W4.454.32E-6SLX5Subunit of the Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex, stimulated by SUMO-modified substrates; contains a RING domain and two SIMs (SUMO-interacting motifs); forms SUMO-dependent nuclear foci, including DNA repair centers

GO enrichment analysis for SGTC_2762
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0978.96E-14SGTC_10462001-0062 216.0 μMChemDiv (Drug-like library)7397780.121622
0.0782.21E-9SGTC_29929074591 71.4 μMChembridge (Drug-like library)177413210.157143
0.0772.75E-9SGTC_1548st061468 90.8 μMTimTec (Pure natural product library)3907990.0983607
0.0764.21E-9SGTC_8940960-0238 55.9 μMChemDiv (Drug-like library)67419070.164384
0.0758.19E-9SGTC_29799012552 45.5 μMChembridge (Drug-like library)171009050.263158
0.0732.08E-8SGTC_1819st053448 58.6 μMTimTec (Natural product derivative library)28576160.0821918amide catabolism
0.0672.18E-7SGTC_29909074105 71.4 μMChembridge (Drug-like library)65020010.111111
0.0649.73E-7SGTC_6470140-0236 81.0 μMChemDiv (Drug-like library)9908370.0727273
0.0631.36E-6SGTC_13571503-1303 39.5 μMChemDiv (Drug-like library)2928950.078125
0.0613.25E-6SGTC_249418-aminoabieta-8,11,13-triene sulfate 3.7 μMMicrosource (Natural product library)167250760.0540541
0.0603.51E-6SGTC_2767sibutramine 71.5 μMNIH Clinical Collection647650.0615385
0.0604.33E-6SGTC_1747st037496 66.6 μMTimTec (Natural product derivative library)7233050.0793651
0.0588.01E-6SGTC_24655809242 33.2 μMMiscellaneous27317480.108108
0.0588.16E-6SGTC_1994st072947 40.9 μMTimTec (Natural product derivative library)52865650.0506329
0.0589.16E-6SGTC_20235144124 124.0 μMChembridge (Fragment library)28301920.0677966

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_22757947463200 μM0.3584911225414Chembridge (Fragment library)216.235880.85813iron homeostasis
SGTC_1463k081-003234.1 μM0.339286977755ChemDiv (Drug-like library)345.114633.15415
SGTC_3008907891271.43 μM0.33928616648731Chembridge (Drug-like library)287.310482.21314
SGTC_21245246320200 μM0.326531579342Chembridge (Fragment library)216.2110631.66913heme biosynthesis & mitochondrial translocase
SGTC_29087930301100 μM0.3191492223178Chembridge (Drug-like library)387.431143.00533
SGTC_3298911685871.43 μM0.3157892506942Chembridge (Drug-like library)279.718862.65713
SGTC_2872904403725.97 μM0.3137266465195Chembridge (Drug-like library)284.1131234.14912
SGTC_1465k213-0056144 μM0.31147524048857ChemDiv (Drug-like library)431.871024.6124DNA intercalators
SGTC_2902578700660.46 μM0.310345591003Chembridge (Drug-like library)309.240013.32516Golgi
SGTC_2967908953849.85 μM0.310345728216Chembridge (Drug-like library)300.663633.0715