sibutramine

1-[1-(4-chlorophenyl)cyclobutyl]-N,N,3-trimethylbutan-1-amine hydrate hydrochloride

A serotonin-norepinephrine reuptake inhibitor. 

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 

PubChem MeSH terms: Antidepressive Agents;Appetite Depressants



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2767
Screen concentration 71.5 μM
Source NIH Clinical Collection
PubChem CID 64765
SMILES CC(C)CC(C1(CCC1)C2=CC=C(C=C2)Cl)N(C)C.O.Cl
Standardized SMILES CC(C)CC(N(C)C)C1(CCC1)c2ccc(Cl)cc2
Molecular weight 334.3243
ALogP 5.35
H-bond donor count 2
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.74
% growth inhibition (Hom. pool) 0


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 64765
Download HIP data (tab-delimited text)  (excel)
Gene:FOL2(YGR267C)|FD-Score:-4.71|P-value:1.21E-6|Clearance:0||SGD DESC:GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway Gene:LAS17(YOR181W)|FD-Score:3.35|P-value:4.06E-4|Clearance:0.03||SGD DESC:Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP) Gene:RIB7(YBR153W)|FD-Score:3.11|P-value:9.24E-4|Clearance:0.03||SGD DESC:Diaminohydroxyphoshoribosylaminopyrimidine deaminase; catalyzes the second step of the riboflavin biosynthesis pathway Gene:RPN6(YDL097C)|FD-Score:-3.2|P-value:6.80E-4|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:SAM35(YHR083W)|FD-Score:-4.23|P-value:1.19E-5|Clearance:0||SGD DESC:Essential component of the sorting and assembly machinery (SAM complex or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane Gene:SSL1(YLR005W)|FD-Score:4.07|P-value:2.31E-5|Clearance:0.73||SGD DESC:Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p Gene:YDL196W(YDL196W_d)|FD-Score:3.31|P-value:4.60E-4|Clearance:0.2||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YKL153W(YKL153W_d)|FD-Score:-3.29|P-value:4.95E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant Gene:FOL2(YGR267C)|FD-Score:-4.71|P-value:1.21E-6|Clearance:0||SGD DESC:GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway Gene:LAS17(YOR181W)|FD-Score:3.35|P-value:4.06E-4|Clearance:0.03||SGD DESC:Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP) Gene:RIB7(YBR153W)|FD-Score:3.11|P-value:9.24E-4|Clearance:0.03||SGD DESC:Diaminohydroxyphoshoribosylaminopyrimidine deaminase; catalyzes the second step of the riboflavin biosynthesis pathway Gene:RPN6(YDL097C)|FD-Score:-3.2|P-value:6.80E-4|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:SAM35(YHR083W)|FD-Score:-4.23|P-value:1.19E-5|Clearance:0||SGD DESC:Essential component of the sorting and assembly machinery (SAM complex or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane Gene:SSL1(YLR005W)|FD-Score:4.07|P-value:2.31E-5|Clearance:0.73||SGD DESC:Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p Gene:YDL196W(YDL196W_d)|FD-Score:3.31|P-value:4.60E-4|Clearance:0.2||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YKL153W(YKL153W_d)|FD-Score:-3.29|P-value:4.95E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 64765
Download HOP data (tab-delimited text)  (excel)
Gene:AQY1(YPR192W)|FD-Score:3.83|P-value:6.42E-5||SGD DESC:Spore-specific water channel that mediates the transport of water across cell membranes, developmentally controlled; may play a role in spore maturation, probably by allowing water outflow, may be involved in freeze tolerance Gene:ATG23(YLR431C)|FD-Score:3.2|P-value:6.78E-4||SGD DESC:Peripheral membrane protein required for the cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p Gene:ATG5(YPL149W)|FD-Score:4.99|P-value:2.99E-7||SGD DESC:Conserved protein involved in autophagy and the Cvt pathway; undergoes conjugation with Atg12p to form a complex involved in Atg8p lipidation; conjugated Atg12p also forms a complex with Atg16p that is essential for autophagosome formation Gene:ATP2(YJR121W)|FD-Score:4.64|P-value:1.74E-6||SGD DESC:Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:BUD7(YOR299W)|FD-Score:-3.82|P-value:6.73E-5||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BUD7 has a paralog, BCH1, that arose from the whole genome duplication Gene:CAP2(YIL034C)|FD-Score:4.11|P-value:1.98E-5||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CCC1(YLR220W)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Putative vacuolar Fe2+/Mn2+ transporter; suppresses respiratory deficit of yfh1 mutants, which lack the ortholog of mammalian frataxin, by preventing mitochondrial iron accumulation; relative distribution to the vacuole decreases upon DNA replication stress Gene:CTF8(YHR191C)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion Gene:CTM1(YHR109W)|FD-Score:3.75|P-value:8.91E-5||SGD DESC:Cytochrome c lysine methyltransferase, trimethylates residue 72 of apo-cytochrome c (Cyc1p) in the cytosol; not required for normal respiratory growth Gene:DDR2(YOL052C-A)|FD-Score:3.16|P-value:7.95E-4||SGD DESC:Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication Gene:DSE2(YHR143W)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:ECM33(YBR078W)|FD-Score:-3.31|P-value:4.65E-4||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:ELG1(YOR144C)|FD-Score:-3.44|P-value:2.86E-4||SGD DESC:Subunit of an alternative replication factor C complex important for DNA replication and genome integrity; suppresses spontaneous DNA damage; involved in homologous recombination-mediated repair and telomere homeostasis; required for PCNA (Pol30p) unloading during DNA replication Gene:EPS1(YIL005W)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:ER protein with chaperone and co-chaperone activity, involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family Gene:FEN2(YCR028C)|FD-Score:3.73|P-value:9.49E-5||SGD DESC:Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:FMP40(YPL222W_p)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Putative protein of unknown function; proposed to be involved in responding to environmental stresses; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GAS5(YOL030W)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:1,3-beta-glucanosyltransferase, has similarity to Gas1p; localizes to the cell wall Gene:GPX1(YKL026C)|FD-Score:3.24|P-value:5.92E-4||SGD DESC:Phospholipid hydroperoxide glutathione peroxidase induced by glucose starvation that protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress Gene:GTO3(YMR251W)|FD-Score:5.34|P-value:4.66E-8||SGD DESC:Omega class glutathione transferase; putative cytosolic localization Gene:GUD1(YDL238C)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Guanine deaminase, a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures Gene:HOM3(YER052C)|FD-Score:-4|P-value:3.13E-5||SGD DESC:Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:IRC24(YIR036C_p)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Putative benzil reductase;(GFP)-fusion protein localizes to the cytoplasm and is induced by the DNA-damaging agent MMS; sequence similarity with short-chain dehydrogenase/reductases; null mutant has increased spontaneous Rad52p foci Gene:MET18(YIL128W)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Cia2p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human MMS19 Gene:MMT2(YPL224C)|FD-Score:4.05|P-value:2.60E-5||SGD DESC:Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt1p Gene:MPD1(YOR288C)|FD-Score:3.18|P-value:7.35E-4||SGD DESC:Member of the protein disulfide isomerase (PDI) family; interacts with and inhibits the chaperone activity of Cne1p; MPD1 overexpression in a pdi1 null mutant suppresses defects in Pdi1p functions such as carboxypeptidase Y maturation Gene:MRPL13(YKR006C)|FD-Score:3.73|P-value:9.55E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MRS2(YOR334W)|FD-Score:4.45|P-value:4.22E-6||SGD DESC:Mitochondrial inner membrane Mg(2+) channel, required for maintenance of intramitochondrial Mg(2+) concentrations at the correct level to support splicing of group II introns Gene:MSC7(YHR039C)|FD-Score:-4.61|P-value:2.03E-6||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids Gene:MSH3(YCR092C)|FD-Score:3.87|P-value:5.40E-5||SGD DESC:Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability Gene:MSS2(YDL107W)|FD-Score:4.01|P-value:3.09E-5||SGD DESC:Peripherally bound inner membrane protein of the mitochondrial matrix involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p Gene:OM14(YBR230C)|FD-Score:3.11|P-value:9.26E-4||SGD DESC:Integral mitochondrial outer membrane protein; abundance is decreased in cells grown in glucose relative to other carbon sources; appears to contain 3 alpha-helical transmembrane segments; ORF encodes a 97-basepair intron Gene:PAD1(YDR538W)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:Phenylacrylic acid decarboxylase, confers resistance to cinnamic acid, decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives; also has mRNA binding activity; homolog of E. coli UbiX Gene:PAU11(YGL261C_p)|FD-Score:3.2|P-value:6.99E-4||SGD DESC:Putative protein of unknown function and member of the seripauperin multigene family encoded mainly in subtelomeric regions; mRNA expression appears to be regulated by SUT1 and UPC2 Gene:PHO85(YPL031C)|FD-Score:4.32|P-value:7.94E-6||SGD DESC:Cyclin-dependent kinase, with ten cyclin partners; involved in regulating the cellular response to nutrient levels and environmental conditions and progression through the cell cycle Gene:PKH2(YOL100W)|FD-Score:-3.76|P-value:8.37E-5||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p Gene:PST1(YDR055W)|FD-Score:-3.11|P-value:9.45E-4||SGD DESC:Cell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1 Gene:PUT3(YKL015W)|FD-Score:5.95|P-value:1.33E-9||SGD DESC:Transcriptional activator of proline utilization genes, constitutively binds PUT1 and PUT2 promoter sequences as a dimer and undergoes a conformational change to form the active state; differentially phosphorylated in the presence of different nitrogen sources; has a Zn(2)-Cys(6) binuclear cluster domain Gene:PXL1(YKR090W)|FD-Score:4.41|P-value:5.10E-6||SGD DESC:Protein that localizes to sites of polarized growth; required for selection and/or maintenance of polarized growth sites, may modulate signaling by the GTPases Cdc42p and Rho1p; contains LIM domains and has similarity to metazoan paxillin; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RAV1(YJR033C)|FD-Score:5.44|P-value:2.63E-8||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:RHO4(YKR055W)|FD-Score:-3.37|P-value:3.81E-4||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely to be involved in the establishment of cell polarity Gene:RPH1(YER169W)|FD-Score:-3.64|P-value:1.39E-4||SGD DESC:JmjC domain-containing histone demethylase; specifically demethylates H3K36 tri- and dimethyl modification states; associates with actively transcribed (RNA polymerase II) regions in vivo and specifically targets H3K36 in its trimethylation state as its substrate; transcriptional repressor of PHR1; Rph1p phosphorylation during DNA damage is under control of the MEC1-RAD53 pathway; RPH1 has a paralog, GIS1, that arose from the whole genome duplication Gene:RTF1(YGL244W)|FD-Score:4.41|P-value:5.14E-6||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:SCW10(YMR305C)|FD-Score:3.12|P-value:9.00E-4||SGD DESC:Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on mutant phenotype and its regulation by Ste12p Gene:SET2(YJL168C)|FD-Score:3.18|P-value:7.35E-4||SGD DESC:Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36, which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain of Rpo21p; histone methylation activity is regulated by phosphorylation status of Rpo21p Gene:SGS1(YMR190C)|FD-Score:-3.61|P-value:1.54E-4||SGD DESC:Nucleolar DNA helicase of the RecQ family; involved in genome integrity maintenance; regulates chromosome synapsis and meiotic joint molecule/crossover formation; potential role as repressor of a subset of rapamycin responsive genes; rapidly lost in response to rapamycin in Rrd1p-dependent manner; similar to human BLM and WRN proteins implicated in Bloom and Werner syndromes; forms nuclear foci upon DNA replication stress Gene:SIC1(YLR079W)|FD-Score:-4.19|P-value:1.40E-5||SGD DESC:Cyclin-dependent kinase inhibitor (CKI); inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1 Gene:SIP18(YMR175W)|FD-Score:4.25|P-value:1.06E-5||SGD DESC:Phospholipid-binding hydrophilin; essential to overcome desiccation-rehydration process; expression is induced by osmotic stress Gene:SPO12(YHR152W)|FD-Score:4.7|P-value:1.31E-6||SGD DESC:Nucleolar protein of unknown function, positive regulator of mitotic exit; involved in regulating release of Cdc14p from the nucleolus in early anaphase, may play similar role in meiosis Gene:SRL4(YPL033C)|FD-Score:3.23|P-value:6.24E-4||SGD DESC:Protein of unknown function; involved in regulation of dNTP production; null mutant suppresses the lethality of lcd1 and rad53 mutations; expression is induced by Kar4p Gene:TDH2(YJR009C)|FD-Score:-3.26|P-value:5.52E-4||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress; TDH2 has a paralog, TDH3, that arose from the whole genome duplication Gene:TPN1(YGL186C)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRR2(YHR106W)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Mitochondrial thioredoxin reductase; involved in protection against oxidative stress, required with Glr1p to maintain the redox state of Trx3p; contains active-site motif (CAVC) present in prokaryotic orthologs; binds NADPH and FAD; TRR2 has a paralog, TRR1, that arose from the whole genome duplication Gene:UBP1(YDL122W)|FD-Score:3.1|P-value:9.57E-4||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains Gene:UBX2(YML013W)|FD-Score:4.7|P-value:1.27E-6||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:UGA2(YBR006W)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:WSC3(YOL105C)|FD-Score:-3.2|P-value:6.76E-4||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity; involved in the response to heat shock and other stressors; regulates 1,3-beta-glucan synthesis Gene:YAP1(YML007W)|FD-Score:-3.17|P-value:7.73E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YBR013C(YBR013C_p)|FD-Score:7.17|P-value:3.76E-13||SGD DESC:Putative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YBR238C(YBR238C)|FD-Score:-3.69|P-value:1.10E-4||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YDC1(YPL087W)|FD-Score:-5.43|P-value:2.89E-8||SGD DESC:Alkaline dihydroceramidase, involved in sphingolipid metabolism; preferentially hydrolyzes dihydroceramide to a free fatty acid and dihydrosphingosine; has a minor reverse activity; YDC1 has a paralog, YPC1, that arose from the whole genome duplication Gene:YDL071C(YDL071C_d)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF BDF2/YDL070W Gene:YER097W(YER097W_d)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER135C(YER135C_d)|FD-Score:-3.14|P-value:8.59E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; YER135C is not an essential gene Gene:YGL242C(YGL242C_p)|FD-Score:6.51|P-value:3.67E-11||SGD DESC:Putative protein of unknown function; deletion mutant is viable Gene:YGR069W(YGR069W_d)|FD-Score:3.22|P-value:6.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR111W(YGR111W_p)|FD-Score:-3.86|P-value:5.56E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YGR168C(YGR168C_p)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Putative protein of unknown function; YGR168C is not an essential gene Gene:YHP1(YDR451C)|FD-Score:3.1|P-value:9.71E-4||SGD DESC:Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication Gene:YKL023W(YKL023W_p)|FD-Score:3.1|P-value:9.68E-4||SGD DESC:Putative protein of unknown function, predicted by computational methods to be involved in mRNA degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YML082W(YML082W_p)|FD-Score:-3.71|P-value:1.04E-4||SGD DESC:Putative protein predicted to have carbon-sulfur lyase activity; transcriptionally regulated by Upc2p via an upstream sterol response element; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and the cytoplasm; YML082W is not an essential gene Gene:YNL193W(YNL193W_p)|FD-Score:3.82|P-value:6.55E-5||SGD DESC:Putative protein of unknown function; exhibits a two-hybrid interaction with Yhr151cp in a large-scale analysis Gene:YNR029C(YNR029C_p)|FD-Score:5.63|P-value:8.88E-9||SGD DESC:Putative protein of unknown function, deletion confers reduced fitness in saline Gene:YOL085C(YOL085C_d)|FD-Score:-3.11|P-value:9.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOL085W-A Gene:YOR316C-A(YOR316C-A_p)|FD-Score:-3.98|P-value:3.46E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPL229W(YPL229W_p)|FD-Score:3.17|P-value:7.54E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YPL229W has a paralog, YMR181C, that arose from the whole genome duplication Gene:YPR063C(YPR063C_p)|FD-Score:4.34|P-value:7.18E-6||SGD DESC:ER-localized protein of unknown function Gene:AQY1(YPR192W)|FD-Score:3.83|P-value:6.42E-5||SGD DESC:Spore-specific water channel that mediates the transport of water across cell membranes, developmentally controlled; may play a role in spore maturation, probably by allowing water outflow, may be involved in freeze tolerance Gene:ATG23(YLR431C)|FD-Score:3.2|P-value:6.78E-4||SGD DESC:Peripheral membrane protein required for the cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p Gene:ATG5(YPL149W)|FD-Score:4.99|P-value:2.99E-7||SGD DESC:Conserved protein involved in autophagy and the Cvt pathway; undergoes conjugation with Atg12p to form a complex involved in Atg8p lipidation; conjugated Atg12p also forms a complex with Atg16p that is essential for autophagosome formation Gene:ATP2(YJR121W)|FD-Score:4.64|P-value:1.74E-6||SGD DESC:Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:BUD7(YOR299W)|FD-Score:-3.82|P-value:6.73E-5||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BUD7 has a paralog, BCH1, that arose from the whole genome duplication Gene:CAP2(YIL034C)|FD-Score:4.11|P-value:1.98E-5||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CCC1(YLR220W)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Putative vacuolar Fe2+/Mn2+ transporter; suppresses respiratory deficit of yfh1 mutants, which lack the ortholog of mammalian frataxin, by preventing mitochondrial iron accumulation; relative distribution to the vacuole decreases upon DNA replication stress Gene:CTF8(YHR191C)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion Gene:CTM1(YHR109W)|FD-Score:3.75|P-value:8.91E-5||SGD DESC:Cytochrome c lysine methyltransferase, trimethylates residue 72 of apo-cytochrome c (Cyc1p) in the cytosol; not required for normal respiratory growth Gene:DDR2(YOL052C-A)|FD-Score:3.16|P-value:7.95E-4||SGD DESC:Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication Gene:DSE2(YHR143W)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:ECM33(YBR078W)|FD-Score:-3.31|P-value:4.65E-4||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:ELG1(YOR144C)|FD-Score:-3.44|P-value:2.86E-4||SGD DESC:Subunit of an alternative replication factor C complex important for DNA replication and genome integrity; suppresses spontaneous DNA damage; involved in homologous recombination-mediated repair and telomere homeostasis; required for PCNA (Pol30p) unloading during DNA replication Gene:EPS1(YIL005W)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:ER protein with chaperone and co-chaperone activity, involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family Gene:FEN2(YCR028C)|FD-Score:3.73|P-value:9.49E-5||SGD DESC:Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:FMP40(YPL222W_p)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Putative protein of unknown function; proposed to be involved in responding to environmental stresses; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GAS5(YOL030W)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:1,3-beta-glucanosyltransferase, has similarity to Gas1p; localizes to the cell wall Gene:GPX1(YKL026C)|FD-Score:3.24|P-value:5.92E-4||SGD DESC:Phospholipid hydroperoxide glutathione peroxidase induced by glucose starvation that protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress Gene:GTO3(YMR251W)|FD-Score:5.34|P-value:4.66E-8||SGD DESC:Omega class glutathione transferase; putative cytosolic localization Gene:GUD1(YDL238C)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Guanine deaminase, a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures Gene:HOM3(YER052C)|FD-Score:-4|P-value:3.13E-5||SGD DESC:Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:IRC24(YIR036C_p)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Putative benzil reductase;(GFP)-fusion protein localizes to the cytoplasm and is induced by the DNA-damaging agent MMS; sequence similarity with short-chain dehydrogenase/reductases; null mutant has increased spontaneous Rad52p foci Gene:MET18(YIL128W)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Cia2p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human MMS19 Gene:MMT2(YPL224C)|FD-Score:4.05|P-value:2.60E-5||SGD DESC:Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt1p Gene:MPD1(YOR288C)|FD-Score:3.18|P-value:7.35E-4||SGD DESC:Member of the protein disulfide isomerase (PDI) family; interacts with and inhibits the chaperone activity of Cne1p; MPD1 overexpression in a pdi1 null mutant suppresses defects in Pdi1p functions such as carboxypeptidase Y maturation Gene:MRPL13(YKR006C)|FD-Score:3.73|P-value:9.55E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MRS2(YOR334W)|FD-Score:4.45|P-value:4.22E-6||SGD DESC:Mitochondrial inner membrane Mg(2+) channel, required for maintenance of intramitochondrial Mg(2+) concentrations at the correct level to support splicing of group II introns Gene:MSC7(YHR039C)|FD-Score:-4.61|P-value:2.03E-6||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids Gene:MSH3(YCR092C)|FD-Score:3.87|P-value:5.40E-5||SGD DESC:Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability Gene:MSS2(YDL107W)|FD-Score:4.01|P-value:3.09E-5||SGD DESC:Peripherally bound inner membrane protein of the mitochondrial matrix involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p Gene:OM14(YBR230C)|FD-Score:3.11|P-value:9.26E-4||SGD DESC:Integral mitochondrial outer membrane protein; abundance is decreased in cells grown in glucose relative to other carbon sources; appears to contain 3 alpha-helical transmembrane segments; ORF encodes a 97-basepair intron Gene:PAD1(YDR538W)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:Phenylacrylic acid decarboxylase, confers resistance to cinnamic acid, decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives; also has mRNA binding activity; homolog of E. coli UbiX Gene:PAU11(YGL261C_p)|FD-Score:3.2|P-value:6.99E-4||SGD DESC:Putative protein of unknown function and member of the seripauperin multigene family encoded mainly in subtelomeric regions; mRNA expression appears to be regulated by SUT1 and UPC2 Gene:PHO85(YPL031C)|FD-Score:4.32|P-value:7.94E-6||SGD DESC:Cyclin-dependent kinase, with ten cyclin partners; involved in regulating the cellular response to nutrient levels and environmental conditions and progression through the cell cycle Gene:PKH2(YOL100W)|FD-Score:-3.76|P-value:8.37E-5||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p Gene:PST1(YDR055W)|FD-Score:-3.11|P-value:9.45E-4||SGD DESC:Cell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1 Gene:PUT3(YKL015W)|FD-Score:5.95|P-value:1.33E-9||SGD DESC:Transcriptional activator of proline utilization genes, constitutively binds PUT1 and PUT2 promoter sequences as a dimer and undergoes a conformational change to form the active state; differentially phosphorylated in the presence of different nitrogen sources; has a Zn(2)-Cys(6) binuclear cluster domain Gene:PXL1(YKR090W)|FD-Score:4.41|P-value:5.10E-6||SGD DESC:Protein that localizes to sites of polarized growth; required for selection and/or maintenance of polarized growth sites, may modulate signaling by the GTPases Cdc42p and Rho1p; contains LIM domains and has similarity to metazoan paxillin; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RAV1(YJR033C)|FD-Score:5.44|P-value:2.63E-8||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:RHO4(YKR055W)|FD-Score:-3.37|P-value:3.81E-4||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely to be involved in the establishment of cell polarity Gene:RPH1(YER169W)|FD-Score:-3.64|P-value:1.39E-4||SGD DESC:JmjC domain-containing histone demethylase; specifically demethylates H3K36 tri- and dimethyl modification states; associates with actively transcribed (RNA polymerase II) regions in vivo and specifically targets H3K36 in its trimethylation state as its substrate; transcriptional repressor of PHR1; Rph1p phosphorylation during DNA damage is under control of the MEC1-RAD53 pathway; RPH1 has a paralog, GIS1, that arose from the whole genome duplication Gene:RTF1(YGL244W)|FD-Score:4.41|P-value:5.14E-6||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:SCW10(YMR305C)|FD-Score:3.12|P-value:9.00E-4||SGD DESC:Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on mutant phenotype and its regulation by Ste12p Gene:SET2(YJL168C)|FD-Score:3.18|P-value:7.35E-4||SGD DESC:Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36, which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain of Rpo21p; histone methylation activity is regulated by phosphorylation status of Rpo21p Gene:SGS1(YMR190C)|FD-Score:-3.61|P-value:1.54E-4||SGD DESC:Nucleolar DNA helicase of the RecQ family; involved in genome integrity maintenance; regulates chromosome synapsis and meiotic joint molecule/crossover formation; potential role as repressor of a subset of rapamycin responsive genes; rapidly lost in response to rapamycin in Rrd1p-dependent manner; similar to human BLM and WRN proteins implicated in Bloom and Werner syndromes; forms nuclear foci upon DNA replication stress Gene:SIC1(YLR079W)|FD-Score:-4.19|P-value:1.40E-5||SGD DESC:Cyclin-dependent kinase inhibitor (CKI); inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1 Gene:SIP18(YMR175W)|FD-Score:4.25|P-value:1.06E-5||SGD DESC:Phospholipid-binding hydrophilin; essential to overcome desiccation-rehydration process; expression is induced by osmotic stress Gene:SPO12(YHR152W)|FD-Score:4.7|P-value:1.31E-6||SGD DESC:Nucleolar protein of unknown function, positive regulator of mitotic exit; involved in regulating release of Cdc14p from the nucleolus in early anaphase, may play similar role in meiosis Gene:SRL4(YPL033C)|FD-Score:3.23|P-value:6.24E-4||SGD DESC:Protein of unknown function; involved in regulation of dNTP production; null mutant suppresses the lethality of lcd1 and rad53 mutations; expression is induced by Kar4p Gene:TDH2(YJR009C)|FD-Score:-3.26|P-value:5.52E-4||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress; TDH2 has a paralog, TDH3, that arose from the whole genome duplication Gene:TPN1(YGL186C)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRR2(YHR106W)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Mitochondrial thioredoxin reductase; involved in protection against oxidative stress, required with Glr1p to maintain the redox state of Trx3p; contains active-site motif (CAVC) present in prokaryotic orthologs; binds NADPH and FAD; TRR2 has a paralog, TRR1, that arose from the whole genome duplication Gene:UBP1(YDL122W)|FD-Score:3.1|P-value:9.57E-4||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains Gene:UBX2(YML013W)|FD-Score:4.7|P-value:1.27E-6||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:UGA2(YBR006W)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:WSC3(YOL105C)|FD-Score:-3.2|P-value:6.76E-4||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity; involved in the response to heat shock and other stressors; regulates 1,3-beta-glucan synthesis Gene:YAP1(YML007W)|FD-Score:-3.17|P-value:7.73E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YBR013C(YBR013C_p)|FD-Score:7.17|P-value:3.76E-13||SGD DESC:Putative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YBR238C(YBR238C)|FD-Score:-3.69|P-value:1.10E-4||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YDC1(YPL087W)|FD-Score:-5.43|P-value:2.89E-8||SGD DESC:Alkaline dihydroceramidase, involved in sphingolipid metabolism; preferentially hydrolyzes dihydroceramide to a free fatty acid and dihydrosphingosine; has a minor reverse activity; YDC1 has a paralog, YPC1, that arose from the whole genome duplication Gene:YDL071C(YDL071C_d)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF BDF2/YDL070W Gene:YER097W(YER097W_d)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER135C(YER135C_d)|FD-Score:-3.14|P-value:8.59E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; YER135C is not an essential gene Gene:YGL242C(YGL242C_p)|FD-Score:6.51|P-value:3.67E-11||SGD DESC:Putative protein of unknown function; deletion mutant is viable Gene:YGR069W(YGR069W_d)|FD-Score:3.22|P-value:6.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR111W(YGR111W_p)|FD-Score:-3.86|P-value:5.56E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YGR168C(YGR168C_p)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Putative protein of unknown function; YGR168C is not an essential gene Gene:YHP1(YDR451C)|FD-Score:3.1|P-value:9.71E-4||SGD DESC:Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication Gene:YKL023W(YKL023W_p)|FD-Score:3.1|P-value:9.68E-4||SGD DESC:Putative protein of unknown function, predicted by computational methods to be involved in mRNA degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YML082W(YML082W_p)|FD-Score:-3.71|P-value:1.04E-4||SGD DESC:Putative protein predicted to have carbon-sulfur lyase activity; transcriptionally regulated by Upc2p via an upstream sterol response element; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and the cytoplasm; YML082W is not an essential gene Gene:YNL193W(YNL193W_p)|FD-Score:3.82|P-value:6.55E-5||SGD DESC:Putative protein of unknown function; exhibits a two-hybrid interaction with Yhr151cp in a large-scale analysis Gene:YNR029C(YNR029C_p)|FD-Score:5.63|P-value:8.88E-9||SGD DESC:Putative protein of unknown function, deletion confers reduced fitness in saline Gene:YOL085C(YOL085C_d)|FD-Score:-3.11|P-value:9.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOL085W-A Gene:YOR316C-A(YOR316C-A_p)|FD-Score:-3.98|P-value:3.46E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPL229W(YPL229W_p)|FD-Score:3.17|P-value:7.54E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YPL229W has a paralog, YMR181C, that arose from the whole genome duplication Gene:YPR063C(YPR063C_p)|FD-Score:4.34|P-value:7.18E-6||SGD DESC:ER-localized protein of unknown function

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR005W4.072.31E-50.72SSL1Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p
YOR181W3.354.06E-40.03LAS17Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP)
YDL196W_d3.314.60E-40.20YDL196W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
YBR153W3.119.24E-40.03RIB7Diaminohydroxyphoshoribosylaminopyrimidine deaminase; catalyzes the second step of the riboflavin biosynthesis pathway
YKL193C3.080.001020.05SDS22Regulatory subunit of the type 1 protein phosphatase (PP1) Glc7p; whether it functions as a positive or negative regulator of Glc7p is controversial; involved in the regulation of Glc7p nuclear localization and function
YOR254C3.030.001210.16SEC63Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
YMR033W2.870.002050.09ARP9Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YBR088C2.780.002700.01POL30Proliferating cell nuclear antigen (PCNA), functions as the sliding clamp for DNA polymerase delta; may function as a docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair
YPR137W2.770.002790.01RRP9Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein
YAL034C-B_d2.770.002840.15YAL034C-B_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YHR107C2.620.004450.01CDC12Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YGL155W2.610.004570.01CDC43Beta subunit of geranylgeranyltransferase type I, catalyzes geranylgeranylation to the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis
YDR429C2.600.004640.02TIF35eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation
YGR119C2.590.004870.03NUP57FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p)
YOR319W2.550.005360.01HSH49U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM)

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR013C_p7.173.76E-13YBR013C_pPutative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein
YGL242C_p6.513.67E-11YGL242C_pPutative protein of unknown function; deletion mutant is viable
YKL015W5.951.33E-9PUT3Transcriptional activator of proline utilization genes, constitutively binds PUT1 and PUT2 promoter sequences as a dimer and undergoes a conformational change to form the active state; differentially phosphorylated in the presence of different nitrogen sources; has a Zn(2)-Cys(6) binuclear cluster domain
YNR029C_p5.638.88E-9YNR029C_pPutative protein of unknown function, deletion confers reduced fitness in saline
YJR033C5.442.63E-8RAV1Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate
YMR251W5.344.66E-8GTO3Omega class glutathione transferase; putative cytosolic localization
YPL149W4.992.99E-7ATG5Conserved protein involved in autophagy and the Cvt pathway; undergoes conjugation with Atg12p to form a complex involved in Atg8p lipidation; conjugated Atg12p also forms a complex with Atg16p that is essential for autophagosome formation
YML013W4.701.27E-6UBX2Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis
YHR152W4.701.31E-6SPO12Nucleolar protein of unknown function, positive regulator of mitotic exit; involved in regulating release of Cdc14p from the nucleolus in early anaphase, may play similar role in meiosis
YJR121W4.641.74E-6ATP2Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated
YOR334W4.454.22E-6MRS2Mitochondrial inner membrane Mg(2+) channel, required for maintenance of intramitochondrial Mg(2+) concentrations at the correct level to support splicing of group II introns
YKR090W4.415.10E-6PXL1Protein that localizes to sites of polarized growth; required for selection and/or maintenance of polarized growth sites, may modulate signaling by the GTPases Cdc42p and Rho1p; contains LIM domains and has similarity to metazoan paxillin; relocalizes from bud neck to cytoplasm upon DNA replication stress
YGL244W4.415.14E-6RTF1Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay
YPR063C_p4.347.18E-6YPR063C_pER-localized protein of unknown function
YPL031C4.327.94E-6PHO85Cyclin-dependent kinase, with ten cyclin partners; involved in regulating the cellular response to nutrient levels and environmental conditions and progression through the cell cycle

GO enrichment analysis for SGTC_2767
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0723.75E-8SGTC_14173966-0327 90.4 μMChemDiv (Drug-like library)6511180.117647
0.0691.28E-7SGTC_24625162487 171.8 μMMiscellaneous22599860.0769231
0.0622.01E-6SGTC_12380333-0287 48.8 μMChemDiv (Drug-like library)72260.137255Golgi
0.0603.44E-6SGTC_6781218-2179 85.9 μMChemDiv (Drug-like library)68200810.057971RNA pol III & RNase P/MRP
0.0603.51E-6SGTC_2762leflunomide 42.1 μMMiscellaneous38990.0615385
0.0604.44E-6SGTC_11383761-0006 1.0 μMChemDiv (Drug-like library)120610870.0625
0.0596.81E-6SGTC_11403229-1848 3.0 μMChemDiv (Drug-like library)28495250.0655738
0.0561.45E-5SGTC_7764476-4378 112.0 μMChemDiv (Drug-like library)28920080.0666667ERG2
0.0561.50E-5SGTC_9771348-1378 135.0 μMChemDiv (Drug-like library)X9770.0375
0.0534.34E-5SGTC_3234013-0906 34.3 μMChemDiv (Drug-like library)29100860.0897436ERG2
0.0518.17E-5SGTC_1678st019265 80.2 μMTimTec (Natural product derivative library)53670200.049180360S ribosome export
0.0518.19E-5SGTC_5454218-0007 221.0 μMChemDiv (Drug-like library)28405080.0517241
0.0501.09E-4SGTC_29819013952 39.0 μMChembridge (Drug-like library)29963440.0735294
0.0491.54E-4SGTC_553k007-0256 45.7 μMChemDiv (Drug-like library)62870000.0677966heme biosynthesis & mitochondrial translocase
0.0482.05E-4SGTC_3542783-3500 4.9 μMChemDiv (Drug-like library)67407610.0625cell wall

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_12430416-00035.65 μM0.3181822141977ChemDiv (Drug-like library)286.795865.68111PDR1
SGTC_484loperamide105 μM0.2656253955Miscellaneous477.037524.63513
SGTC_20194016004108 μM0.25531945595336Chembridge (Fragment library)295.743161.06545
SGTC_2784092-082123.9 μM0.234043877900ChemDiv (Drug-like library)328.87895.05102plasma membrane duress
SGTC_2249haloperidol116.36 μM0.2222223559Miscellaneous375.8642233.88714ergosterol depletion effects on membrane
SGTC_258haloperidol50.78 μM0.2222223559Miscellaneous375.8642233.88714fatty acid desaturase (OLE1)
SGTC_2725haloperidol53.21 μM0.2222223559Miscellaneous375.8642233.88714plasma membrane duress
SGTC_3012908043371.43 μM0.21311517688859Chembridge (Drug-like library)321.80193.26604
SGTC_12440416-001552.5 μM0.20930296201ChemDiv (Drug-like library)254.323624.31922ERG2
SGTC_6464092-083927.3 μM0.207547877796ChemDiv (Drug-like library)306.873384.83902plasma membrane duress
SGTC_13071187-159912.2 μM0.2075471538471ChemDiv (Drug-like library)296.403365.3512PDR1
SGTC_23639053361176 μM0.2037046469797Chembridge (Fragment library)260.718822.51212Golgi