toltrazuril FDA approved compound

1-methyl-3-[3-methyl-4-[4-(trifluoromethylsulfanyl)phenoxy]phenyl]-1,3,5-triazinane-2,4,6-trione

Kills coccidiodes.

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PubChem MeSH terms: Coccidiostats



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2770
Screen concentration 17.5 μM
Source Miscellaneous
PubChem CID 68591
SMILES CC1=C(C=CC(=C1)N2C(=O)NC(=O)N(C2=O)C)OC3=CC=C(C=C3)SC(F)(F)F
Standardized SMILES CN1C(=O)NC(=O)N(C1=O)c2ccc(Oc3ccc(SC(F)(F)F)cc3)c(C)c2
Molecular weight 425.3817
ALogP 6.05
H-bond donor count 1
H-bond acceptor count 8
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.74
% growth inhibition (Hom. pool) 1.22


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 68591
Download HIP data (tab-delimited text)  (excel)
Gene:BDP1(YNL039W)|FD-Score:-3.23|P-value:6.15E-4|Clearance:0||SGD DESC:Essential subunit of RNA polymerase III transcription factor (TFIIIB), which is involved in transcription of genes encoding tRNAs, 5S rRNA, U6 snRNA, and other small RNAs Gene:EFB1(YAL003W)|FD-Score:3.14|P-value:8.40E-4|Clearance:0.33||SGD DESC:Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site Gene:FCF2(YLR051C)|FD-Score:-3.8|P-value:7.27E-5|Clearance:0||SGD DESC:Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon Gene:GPI11(YDR302W)|FD-Score:-4.16|P-value:1.59E-5|Clearance:0||SGD DESC:ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog Gene:GPI18(YBR004C)|FD-Score:3.23|P-value:6.15E-4|Clearance:0.06||SGD DESC:Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria Gene:MAS2(YHR024C)|FD-Score:-3.31|P-value:4.71E-4|Clearance:0||SGD DESC:Larger subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:PRI2(YKL045W)|FD-Score:-3.22|P-value:6.35E-4|Clearance:0||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:RIB7(YBR153W)|FD-Score:4.27|P-value:9.75E-6|Clearance:0.21||SGD DESC:Diaminohydroxyphoshoribosylaminopyrimidine deaminase; catalyzes the second step of the riboflavin biosynthesis pathway Gene:RPB8(YOR224C)|FD-Score:-3.64|P-value:1.36E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:STH1(YIL126W)|FD-Score:-3.4|P-value:3.34E-4|Clearance:0||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:UTP11(YKL099C)|FD-Score:3.18|P-value:7.46E-4|Clearance:0.03||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:UTP18(YJL069C)|FD-Score:4.06|P-value:2.45E-5|Clearance:0.83||SGD DESC:Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data Gene:YDR413C(YDR413C_d)|FD-Score:5.92|P-value:1.61E-9|Clearance:1.65||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing Gene:YGR073C(YGR073C_d)|FD-Score:-3.64|P-value:1.34E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein Gene:YMR290W-A(YMR290W-A_d)|FD-Score:-3.65|P-value:1.29E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:YPD1(YDL235C)|FD-Score:-3.66|P-value:1.28E-4|Clearance:0||SGD DESC:Phosphorelay intermediate protein, phosphorylated by the plasma membrane sensor Sln1p in response to osmotic stress and then in turn phosphorylates the response regulators Ssk1p in the cytosol and Skn7p in the nucleus Gene:YPI1(YFR003C)|FD-Score:-4.66|P-value:1.57E-6|Clearance:0||SGD DESC:Regulatory subunit of the type I protein phosphatase (PP1) Glc7p; Glc7p participates in the regulation of a variety of metabolic processes including mitosis and glycogen metabolism; in vitro evidence suggests Ypi1p is an inhibitor of Glc7p while in vivo evidence suggests it is an activator; overproduction causes decreased cellular content of glycogen; partial depletion causes lithium sensitivity, while overproduction confers lithium-tolerance Gene:BDP1(YNL039W)|FD-Score:-3.23|P-value:6.15E-4|Clearance:0||SGD DESC:Essential subunit of RNA polymerase III transcription factor (TFIIIB), which is involved in transcription of genes encoding tRNAs, 5S rRNA, U6 snRNA, and other small RNAs Gene:EFB1(YAL003W)|FD-Score:3.14|P-value:8.40E-4|Clearance:0.33||SGD DESC:Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site Gene:FCF2(YLR051C)|FD-Score:-3.8|P-value:7.27E-5|Clearance:0||SGD DESC:Essential nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon Gene:GPI11(YDR302W)|FD-Score:-4.16|P-value:1.59E-5|Clearance:0||SGD DESC:ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog Gene:GPI18(YBR004C)|FD-Score:3.23|P-value:6.15E-4|Clearance:0.06||SGD DESC:Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria Gene:MAS2(YHR024C)|FD-Score:-3.31|P-value:4.71E-4|Clearance:0||SGD DESC:Larger subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:PRI2(YKL045W)|FD-Score:-3.22|P-value:6.35E-4|Clearance:0||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:RIB7(YBR153W)|FD-Score:4.27|P-value:9.75E-6|Clearance:0.21||SGD DESC:Diaminohydroxyphoshoribosylaminopyrimidine deaminase; catalyzes the second step of the riboflavin biosynthesis pathway Gene:RPB8(YOR224C)|FD-Score:-3.64|P-value:1.36E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:STH1(YIL126W)|FD-Score:-3.4|P-value:3.34E-4|Clearance:0||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:UTP11(YKL099C)|FD-Score:3.18|P-value:7.46E-4|Clearance:0.03||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:UTP18(YJL069C)|FD-Score:4.06|P-value:2.45E-5|Clearance:0.83||SGD DESC:Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data Gene:YDR413C(YDR413C_d)|FD-Score:5.92|P-value:1.61E-9|Clearance:1.65||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing Gene:YGR073C(YGR073C_d)|FD-Score:-3.64|P-value:1.34E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein Gene:YMR290W-A(YMR290W-A_d)|FD-Score:-3.65|P-value:1.29E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:YPD1(YDL235C)|FD-Score:-3.66|P-value:1.28E-4|Clearance:0||SGD DESC:Phosphorelay intermediate protein, phosphorylated by the plasma membrane sensor Sln1p in response to osmotic stress and then in turn phosphorylates the response regulators Ssk1p in the cytosol and Skn7p in the nucleus Gene:YPI1(YFR003C)|FD-Score:-4.66|P-value:1.57E-6|Clearance:0||SGD DESC:Regulatory subunit of the type I protein phosphatase (PP1) Glc7p; Glc7p participates in the regulation of a variety of metabolic processes including mitosis and glycogen metabolism; in vitro evidence suggests Ypi1p is an inhibitor of Glc7p while in vivo evidence suggests it is an activator; overproduction causes decreased cellular content of glycogen; partial depletion causes lithium sensitivity, while overproduction confers lithium-tolerance

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 68591
Download HOP data (tab-delimited text)  (excel)
Gene:AAD6(YFL056C)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin Gene:AEP2(YMR282C)|FD-Score:-3.22|P-value:6.35E-4||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:AHA1(YDR214W)|FD-Score:4.05|P-value:2.52E-5||SGD DESC:Co-chaperone that binds to Hsp82p and activates its ATPase activity; similar to Hch1p; expression is regulated by stresses such as heat shock; protein abundance increases in response to DNA replication stress Gene:ATG27(YJL178C)|FD-Score:3.23|P-value:6.15E-4||SGD DESC:Type I membrane protein involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; may be involved in membrane delivery to the phagophore assembly site Gene:BAS1(YKR099W)|FD-Score:3.21|P-value:6.71E-4||SGD DESC:Myb-related transcription factor involved in regulating basal and induced expression of genes of the purine and histidine biosynthesis pathways; also involved in regulation of meiotic recombination at specific genes Gene:CLA4(YNL298W)|FD-Score:3.27|P-value:5.30E-4||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:COG5(YNL051W)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:9.43|P-value:2.07E-21||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:8.05|P-value:4.25E-16||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX9(YDL067C)|FD-Score:3.88|P-value:5.16E-5||SGD DESC:Subunit VIIa of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CUS2(YNL286W)|FD-Score:-3.1|P-value:9.65E-4||SGD DESC:Protein that binds to U2 snRNA and Prp11p, may be involved in U2 snRNA folding; contains two RNA recognition motifs (RRMs) Gene:CWP2(YKL096W-A)|FD-Score:4.11|P-value:1.94E-5||SGD DESC:Covalently linked cell wall mannoprotein, major constituent of the cell wall; plays a role in stabilizing the cell wall; involved in low pH resistance; precursor is GPI-anchored Gene:DAL3(YIR032C)|FD-Score:3.24|P-value:6.00E-4||SGD DESC:Ureidoglycolate hydrolase, converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:DAL81(YIR023W)|FD-Score:3.23|P-value:6.15E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DYN3(YMR299C)|FD-Score:3.9|P-value:4.82E-5||SGD DESC:Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration Gene:EAP1(YKL204W)|FD-Score:-4.32|P-value:7.69E-6||SGD DESC:eIF4E-associated protein, competes with eIF4G for binding to eIF4E; accelerates mRNA degradation by promoting decapping, facilitated by interaction with eIF4E; essential for Puf5p mediated repression; associates with Puf5p and Dhh1p; inhibits cap-dependent translation; functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade Gene:EIS1(YMR031C)|FD-Score:-3.27|P-value:5.29E-4||SGD DESC:Component of the eisosome required for proper eisosome assembly; similarity to Ykl050cp and Uso1p; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:EOS1(YNL080C)|FD-Score:-3.21|P-value:6.54E-4||SGD DESC:Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene Gene:FEN2(YCR028C)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:FET4(YMR319C)|FD-Score:5.4|P-value:3.28E-8||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:GAT2(YMR136W)|FD-Score:3.94|P-value:4.02E-5||SGD DESC:Protein containing GATA family zinc finger motifs; similar to Gln3p and Dal80p; expression repressed by leucine Gene:GEP7(YGL057C)|FD-Score:-3.25|P-value:5.67E-4||SGD DESC:Protein of unknown function; null mutant exhibits a respiratory growth defect and synthetic interactions with prohibitin (phb1) and gem1; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GRE1(YPL223C)|FD-Score:-3.24|P-value:5.94E-4||SGD DESC:Hydrophilin essential in dessication-rehydration process; stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway Gene:HBT1(YDL223C)|FD-Score:4.12|P-value:1.87E-5||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:HNT3(YOR258W)|FD-Score:-4.1|P-value:2.10E-5||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:HOM3(YER052C)|FD-Score:4.41|P-value:5.19E-6||SGD DESC:Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:IKI3(YLR384C)|FD-Score:6.3|P-value:1.51E-10||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD) Gene:IMH1(YLR309C)|FD-Score:3.72|P-value:9.86E-5||SGD DESC:Protein involved in vesicular transport, mediates transport between an endosomal compartment and the Golgi, contains a Golgi-localization (GRIP) domain that interacts with activated Arl1p-GTP to localize Imh1p to the Golgi Gene:IRS4(YKR019C)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ISC10(YER180C)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells Gene:MAM3(YOL060C)|FD-Score:6.49|P-value:4.34E-11||SGD DESC:Protein required for normal mitochondrial morphology, has similarity to hemolysins Gene:MEP1(YGR121C)|FD-Score:-3.49|P-value:2.43E-4||SGD DESC:Ammonium permease; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation Gene:MNR2(YKL064W)|FD-Score:3.86|P-value:5.77E-5||SGD DESC:Vacuolar membrane protein required for magnesium homeostasis; putative magnesium transporter; has similarity to Alr1p and Alr2p, which mediate influx of Mg2+ and other divalent cations Gene:MPM1(YJL066C)|FD-Score:-3.36|P-value:3.96E-4||SGD DESC:Mitochondrial intermembrane space protein of unknown function Gene:MTC2(YKL098W)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Protein of unknown function; mtc2 is synthetically sick with cdc13-1 Gene:MTD1(YKR080W)|FD-Score:-3.61|P-value:1.53E-4||SGD DESC:NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase, plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline Gene:NAM2(YLR382C)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance Gene:NCR1(YPL006W)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Vacuolar membrane protein that transits through the biosynthetic vacuolar protein sorting pathway, involved in sphingolipid metabolism; glycoprotein and functional orthologue of human Niemann Pick C1 (NPC1) protein Gene:NMA1(YLR328W)|FD-Score:-3.24|P-value:6.07E-4||SGD DESC:Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in pathways of NAD biosynthesis, including the de novo, NAD(+) salvage, and nicotinamide riboside salvage pathways; NMA1 has a paralog, NMA2, that arose from the whole genome duplication Gene:NOP6(YDL213C)|FD-Score:3.11|P-value:9.41E-4||SGD DESC:rRNA-binding protein required for 40S ribosomal subunit biogenesis; contains an RNA recognition motif (RRM); hydrophilin essential to overcome the stress of the desiccation-rehydration process; NOP6 may be a fungal-specific gene as no homologs have been yet identified in higher eukaryotes Gene:PDE2(YOR360C)|FD-Score:-3.25|P-value:5.82E-4||SGD DESC:High-affinity cyclic AMP phosphodiesterase, component of the cAMP-dependent protein kinase signaling system, protects the cell from extracellular cAMP, contains readthrough motif surrounding termination codon Gene:PEX22(YAL055W)|FD-Score:-3.87|P-value:5.49E-5||SGD DESC:Putative peroxisomal membrane protein required for import of peroxisomal proteins, functionally complements a Pichia pastoris pex22 mutation Gene:PKP2(YGL059W)|FD-Score:5.49|P-value:2.02E-8||SGD DESC:Mitochondrial protein kinase; negatively regulates activity of the pyruvate dehydrogenase complex by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp1p and phosphatases Ptc5p and Ptc6p; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:PMP3(YDR276C)|FD-Score:4.07|P-value:2.30E-5||SGD DESC:Small plasma membrane protein related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature, not essential for viability, deletion causes hyperpolarization of the plasma membrane potential Gene:PPN1(YDR452W)|FD-Score:-4.35|P-value:6.72E-6||SGD DESC:Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:PTC1(YDL006W)|FD-Score:3.26|P-value:5.54E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:PTC4(YBR125C)|FD-Score:3.85|P-value:5.90E-5||SGD DESC:Cytoplasmic type 2C protein phosphatase (PP2C); identified as a high-copy number suppressor of cnb1 mpk1 synthetic lethality; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity Gene:RDS1(YCR106W)|FD-Score:4.62|P-value:1.94E-6||SGD DESC:Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide Gene:RNY1(YPL123C)|FD-Score:3.86|P-value:5.77E-5||SGD DESC:Vacuolar RNase of the T(2) family, relocalizes to the cytosol where it cleaves tRNAs upon oxidative or stationary phase stress; promotes apoptosis under stress conditions and this function is independent of its catalytic activity Gene:ROX3(YBL093C)|FD-Score:3.3|P-value:4.91E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme Gene:RPL9A(YGL147C)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Ribosomal 60S subunit protein L9A; nearly identical to paralog Rpl9Bp; homologous to mammalian ribosomal protein L9 and bacterial L6 Gene:RPS14B(YJL191W)|FD-Score:-3.21|P-value:6.69E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14B has a paralog, RPS14A, that arose from the whole genome duplication Gene:RPS18B(YML026C)|FD-Score:-3.46|P-value:2.67E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18B has a paralog, RPS18A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RSF2(YJR127C)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Zinc-finger protein; involved in transcriptional control of both nuclear and mitochondrial genes, many of which specify products required for glycerol-based growth, respiration, and other functions; RSF2 has a paralog, TDA9, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:SAM2(YDR502C)|FD-Score:3.97|P-value:3.67E-5||SGD DESC:S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM2 has a paralog, SAM1, that arose from the whole genome duplication Gene:SAY1(YGR263C)|FD-Score:-3.15|P-value:8.22E-4||SGD DESC:Sterol deacetylase; component of the sterol acetylation/deacetylation cycle along with Atf2p; integral membrane protein with active site in the ER lumen; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum Gene:SCT1(YBL011W)|FD-Score:4.31|P-value:8.27E-6||SGD DESC:Glycerol 3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase of the glycerolipid biosynthesis pathway, prefers 16-carbon fatty acids, similar to Gpt2p, gene is constitutively transcribed Gene:SMA2(YML066C)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:Meiosis-specific prospore membrane protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation Gene:SNA4(YDL123W)|FD-Score:-3.12|P-value:8.92E-4||SGD DESC:Protein of unknown function, localized to the vacuolar outer membrane; predicted to be palmitoylated Gene:SPR1(YOR190W)|FD-Score:-3.43|P-value:2.98E-4||SGD DESC:Sporulation-specific exo-1,3-beta-glucanase; contributes to ascospore thermoresistance Gene:SRO9(YCL037C)|FD-Score:4.39|P-value:5.68E-6||SGD DESC:Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication Gene:STE23(YLR389C)|FD-Score:3.97|P-value:3.63E-5||SGD DESC:Metalloprotease involved, with homolog Axl1p, in N-terminal processing of pro-a-factor to the mature form; expressed in both haploids and diploids; member of the insulin-degrading enzyme family Gene:SUR4(YLR372W)|FD-Score:3.82|P-value:6.62E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TDA9(YML081W)|FD-Score:-3.2|P-value:6.87E-4||SGD DESC:DNA-binding protein, putative transcription factor; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; TDA9 has a paralog, RSF2, that arose from the whole genome duplication Gene:TDH2(YJR009C)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress; TDH2 has a paralog, TDH3, that arose from the whole genome duplication Gene:THI7(YLR237W)|FD-Score:-3.1|P-value:9.71E-4||SGD DESC:Plasma membrane transporter responsible for the uptake of thiamine, member of the major facilitator superfamily of transporters; mutation of human ortholog causes thiamine-responsive megaloblastic anemia Gene:TRP3(YKL211C)|FD-Score:5.41|P-value:3.17E-8||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TSC3(YBR058C-A)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBI4(YLL039C)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Ubiquitin; becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-26S proteasome system; essential for the cellular stress response; encoded as a polyubiquitin precursor comprised of 5 head-to-tail repeats; protein abundance increases in response to DNA replication stress Gene:UIP4(YPL186C)|FD-Score:-3.19|P-value:7.07E-4||SGD DESC:Protein that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates; detected in a phosphorylated state in the mitochondrial outer membrane; also detected in ER and nuclear envelope Gene:UNG1(YML021C)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Uracil-DNA glycosylase, required for repair of uracil in DNA formed by spontaneous cytosine deamination, not required for strand-specific mismatch repair, cell-cycle regulated, expressed in late G1, localizes to mitochondria and nucleus Gene:YBL062W(YBL062W_d)|FD-Score:4.64|P-value:1.74E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR238C(YBR238C)|FD-Score:-3.67|P-value:1.21E-4||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YBR242W(YBR242W_p)|FD-Score:-3.21|P-value:6.70E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene Gene:YDL186W(YDL186W_p)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Putative protein of unknown function; YDL186W is not an essential gene Gene:YER135C(YER135C_d)|FD-Score:-3.15|P-value:8.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; YER135C is not an essential gene Gene:YGR237C(YGR237C_p)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YGR273C(YGR273C_p)|FD-Score:4.28|P-value:9.41E-6||SGD DESC:Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene Gene:YIL025C(YIL025C_d)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL108W(YIL108W_p)|FD-Score:4.34|P-value:7.18E-6||SGD DESC:Putative metalloendopeptidase; forms cytoplasmic foci upon DNA replication stress Gene:YIL141W(YIL141W_d)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL027C(YJL027C_p)|FD-Score:7.36|P-value:9.42E-14||SGD DESC:Putative protein of unknown function Gene:YJL132W(YJL132W_p)|FD-Score:3.15|P-value:8.13E-4||SGD DESC:Putative protein of unknown function; localizes to the membrane fraction; possible Zap1p-regulated target gene induced by zinc deficiency; YJL132W is a non-essential gene Gene:YJR038C(YJR038C_d)|FD-Score:4.22|P-value:1.21E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR296W(YLR296W_d)|FD-Score:-5.06|P-value:2.15E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR406C-A(YLR406C-A_p)|FD-Score:-3.52|P-value:2.19E-4||SGD DESC:Putative protein of unknown function Gene:YMR265C(YMR265C_p)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:Putative protein of unknown function Gene:YNR065C(YNR065C_p)|FD-Score:-4|P-value:3.22E-5||SGD DESC:Protein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene Gene:YOL097W-A(YOL097W-A_p)|FD-Score:-3.37|P-value:3.73E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YOR186W(YOR186W_p)|FD-Score:-3.59|P-value:1.68E-4||SGD DESC:Putative protein of unknown function; proper regulation of expression during heat stress is sphingolipid-dependent; YOR186W has a paralog, YLR297W, that arose from the whole genome duplication Gene:YOR263C(YOR263C_d)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF DES3/YOR264W Gene:YOR316C-A(YOR316C-A_p)|FD-Score:4.73|P-value:1.13E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPL136W(YPL136W_d)|FD-Score:4.34|P-value:7.10E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene GIP3/YPL137C Gene:YPR123C(YPR123C_d)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:AAD6(YFL056C)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin Gene:AEP2(YMR282C)|FD-Score:-3.22|P-value:6.35E-4||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:AHA1(YDR214W)|FD-Score:4.05|P-value:2.52E-5||SGD DESC:Co-chaperone that binds to Hsp82p and activates its ATPase activity; similar to Hch1p; expression is regulated by stresses such as heat shock; protein abundance increases in response to DNA replication stress Gene:ATG27(YJL178C)|FD-Score:3.23|P-value:6.15E-4||SGD DESC:Type I membrane protein involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; may be involved in membrane delivery to the phagophore assembly site Gene:BAS1(YKR099W)|FD-Score:3.21|P-value:6.71E-4||SGD DESC:Myb-related transcription factor involved in regulating basal and induced expression of genes of the purine and histidine biosynthesis pathways; also involved in regulation of meiotic recombination at specific genes Gene:CLA4(YNL298W)|FD-Score:3.27|P-value:5.30E-4||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:COG5(YNL051W)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:9.43|P-value:2.07E-21||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:8.05|P-value:4.25E-16||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX9(YDL067C)|FD-Score:3.88|P-value:5.16E-5||SGD DESC:Subunit VIIa of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CUS2(YNL286W)|FD-Score:-3.1|P-value:9.65E-4||SGD DESC:Protein that binds to U2 snRNA and Prp11p, may be involved in U2 snRNA folding; contains two RNA recognition motifs (RRMs) Gene:CWP2(YKL096W-A)|FD-Score:4.11|P-value:1.94E-5||SGD DESC:Covalently linked cell wall mannoprotein, major constituent of the cell wall; plays a role in stabilizing the cell wall; involved in low pH resistance; precursor is GPI-anchored Gene:DAL3(YIR032C)|FD-Score:3.24|P-value:6.00E-4||SGD DESC:Ureidoglycolate hydrolase, converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:DAL81(YIR023W)|FD-Score:3.23|P-value:6.15E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DYN3(YMR299C)|FD-Score:3.9|P-value:4.82E-5||SGD DESC:Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration Gene:EAP1(YKL204W)|FD-Score:-4.32|P-value:7.69E-6||SGD DESC:eIF4E-associated protein, competes with eIF4G for binding to eIF4E; accelerates mRNA degradation by promoting decapping, facilitated by interaction with eIF4E; essential for Puf5p mediated repression; associates with Puf5p and Dhh1p; inhibits cap-dependent translation; functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade Gene:EIS1(YMR031C)|FD-Score:-3.27|P-value:5.29E-4||SGD DESC:Component of the eisosome required for proper eisosome assembly; similarity to Ykl050cp and Uso1p; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:EOS1(YNL080C)|FD-Score:-3.21|P-value:6.54E-4||SGD DESC:Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene Gene:FEN2(YCR028C)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:FET4(YMR319C)|FD-Score:5.4|P-value:3.28E-8||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:GAT2(YMR136W)|FD-Score:3.94|P-value:4.02E-5||SGD DESC:Protein containing GATA family zinc finger motifs; similar to Gln3p and Dal80p; expression repressed by leucine Gene:GEP7(YGL057C)|FD-Score:-3.25|P-value:5.67E-4||SGD DESC:Protein of unknown function; null mutant exhibits a respiratory growth defect and synthetic interactions with prohibitin (phb1) and gem1; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GRE1(YPL223C)|FD-Score:-3.24|P-value:5.94E-4||SGD DESC:Hydrophilin essential in dessication-rehydration process; stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway Gene:HBT1(YDL223C)|FD-Score:4.12|P-value:1.87E-5||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:HNT3(YOR258W)|FD-Score:-4.1|P-value:2.10E-5||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:HOM3(YER052C)|FD-Score:4.41|P-value:5.19E-6||SGD DESC:Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:IKI3(YLR384C)|FD-Score:6.3|P-value:1.51E-10||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD) Gene:IMH1(YLR309C)|FD-Score:3.72|P-value:9.86E-5||SGD DESC:Protein involved in vesicular transport, mediates transport between an endosomal compartment and the Golgi, contains a Golgi-localization (GRIP) domain that interacts with activated Arl1p-GTP to localize Imh1p to the Golgi Gene:IRS4(YKR019C)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ISC10(YER180C)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells Gene:MAM3(YOL060C)|FD-Score:6.49|P-value:4.34E-11||SGD DESC:Protein required for normal mitochondrial morphology, has similarity to hemolysins Gene:MEP1(YGR121C)|FD-Score:-3.49|P-value:2.43E-4||SGD DESC:Ammonium permease; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation Gene:MNR2(YKL064W)|FD-Score:3.86|P-value:5.77E-5||SGD DESC:Vacuolar membrane protein required for magnesium homeostasis; putative magnesium transporter; has similarity to Alr1p and Alr2p, which mediate influx of Mg2+ and other divalent cations Gene:MPM1(YJL066C)|FD-Score:-3.36|P-value:3.96E-4||SGD DESC:Mitochondrial intermembrane space protein of unknown function Gene:MTC2(YKL098W)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Protein of unknown function; mtc2 is synthetically sick with cdc13-1 Gene:MTD1(YKR080W)|FD-Score:-3.61|P-value:1.53E-4||SGD DESC:NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase, plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline Gene:NAM2(YLR382C)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance Gene:NCR1(YPL006W)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Vacuolar membrane protein that transits through the biosynthetic vacuolar protein sorting pathway, involved in sphingolipid metabolism; glycoprotein and functional orthologue of human Niemann Pick C1 (NPC1) protein Gene:NMA1(YLR328W)|FD-Score:-3.24|P-value:6.07E-4||SGD DESC:Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in pathways of NAD biosynthesis, including the de novo, NAD(+) salvage, and nicotinamide riboside salvage pathways; NMA1 has a paralog, NMA2, that arose from the whole genome duplication Gene:NOP6(YDL213C)|FD-Score:3.11|P-value:9.41E-4||SGD DESC:rRNA-binding protein required for 40S ribosomal subunit biogenesis; contains an RNA recognition motif (RRM); hydrophilin essential to overcome the stress of the desiccation-rehydration process; NOP6 may be a fungal-specific gene as no homologs have been yet identified in higher eukaryotes Gene:PDE2(YOR360C)|FD-Score:-3.25|P-value:5.82E-4||SGD DESC:High-affinity cyclic AMP phosphodiesterase, component of the cAMP-dependent protein kinase signaling system, protects the cell from extracellular cAMP, contains readthrough motif surrounding termination codon Gene:PEX22(YAL055W)|FD-Score:-3.87|P-value:5.49E-5||SGD DESC:Putative peroxisomal membrane protein required for import of peroxisomal proteins, functionally complements a Pichia pastoris pex22 mutation Gene:PKP2(YGL059W)|FD-Score:5.49|P-value:2.02E-8||SGD DESC:Mitochondrial protein kinase; negatively regulates activity of the pyruvate dehydrogenase complex by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp1p and phosphatases Ptc5p and Ptc6p; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:PMP3(YDR276C)|FD-Score:4.07|P-value:2.30E-5||SGD DESC:Small plasma membrane protein related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature, not essential for viability, deletion causes hyperpolarization of the plasma membrane potential Gene:PPN1(YDR452W)|FD-Score:-4.35|P-value:6.72E-6||SGD DESC:Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:PTC1(YDL006W)|FD-Score:3.26|P-value:5.54E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:PTC4(YBR125C)|FD-Score:3.85|P-value:5.90E-5||SGD DESC:Cytoplasmic type 2C protein phosphatase (PP2C); identified as a high-copy number suppressor of cnb1 mpk1 synthetic lethality; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity Gene:RDS1(YCR106W)|FD-Score:4.62|P-value:1.94E-6||SGD DESC:Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide Gene:RNY1(YPL123C)|FD-Score:3.86|P-value:5.77E-5||SGD DESC:Vacuolar RNase of the T(2) family, relocalizes to the cytosol where it cleaves tRNAs upon oxidative or stationary phase stress; promotes apoptosis under stress conditions and this function is independent of its catalytic activity Gene:ROX3(YBL093C)|FD-Score:3.3|P-value:4.91E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme Gene:RPL9A(YGL147C)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Ribosomal 60S subunit protein L9A; nearly identical to paralog Rpl9Bp; homologous to mammalian ribosomal protein L9 and bacterial L6 Gene:RPS14B(YJL191W)|FD-Score:-3.21|P-value:6.69E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14B has a paralog, RPS14A, that arose from the whole genome duplication Gene:RPS18B(YML026C)|FD-Score:-3.46|P-value:2.67E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18B has a paralog, RPS18A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RSF2(YJR127C)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Zinc-finger protein; involved in transcriptional control of both nuclear and mitochondrial genes, many of which specify products required for glycerol-based growth, respiration, and other functions; RSF2 has a paralog, TDA9, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:SAM2(YDR502C)|FD-Score:3.97|P-value:3.67E-5||SGD DESC:S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM2 has a paralog, SAM1, that arose from the whole genome duplication Gene:SAY1(YGR263C)|FD-Score:-3.15|P-value:8.22E-4||SGD DESC:Sterol deacetylase; component of the sterol acetylation/deacetylation cycle along with Atf2p; integral membrane protein with active site in the ER lumen; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum Gene:SCT1(YBL011W)|FD-Score:4.31|P-value:8.27E-6||SGD DESC:Glycerol 3-phosphate/dihydroxyacetone phosphate dual substrate-specific sn-1 acyltransferase of the glycerolipid biosynthesis pathway, prefers 16-carbon fatty acids, similar to Gpt2p, gene is constitutively transcribed Gene:SMA2(YML066C)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:Meiosis-specific prospore membrane protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation Gene:SNA4(YDL123W)|FD-Score:-3.12|P-value:8.92E-4||SGD DESC:Protein of unknown function, localized to the vacuolar outer membrane; predicted to be palmitoylated Gene:SPR1(YOR190W)|FD-Score:-3.43|P-value:2.98E-4||SGD DESC:Sporulation-specific exo-1,3-beta-glucanase; contributes to ascospore thermoresistance Gene:SRO9(YCL037C)|FD-Score:4.39|P-value:5.68E-6||SGD DESC:Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication Gene:STE23(YLR389C)|FD-Score:3.97|P-value:3.63E-5||SGD DESC:Metalloprotease involved, with homolog Axl1p, in N-terminal processing of pro-a-factor to the mature form; expressed in both haploids and diploids; member of the insulin-degrading enzyme family Gene:SUR4(YLR372W)|FD-Score:3.82|P-value:6.62E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:TDA9(YML081W)|FD-Score:-3.2|P-value:6.87E-4||SGD DESC:DNA-binding protein, putative transcription factor; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; TDA9 has a paralog, RSF2, that arose from the whole genome duplication Gene:TDH2(YJR009C)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress; TDH2 has a paralog, TDH3, that arose from the whole genome duplication Gene:THI7(YLR237W)|FD-Score:-3.1|P-value:9.71E-4||SGD DESC:Plasma membrane transporter responsible for the uptake of thiamine, member of the major facilitator superfamily of transporters; mutation of human ortholog causes thiamine-responsive megaloblastic anemia Gene:TRP3(YKL211C)|FD-Score:5.41|P-value:3.17E-8||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TSC3(YBR058C-A)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBI4(YLL039C)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Ubiquitin; becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-26S proteasome system; essential for the cellular stress response; encoded as a polyubiquitin precursor comprised of 5 head-to-tail repeats; protein abundance increases in response to DNA replication stress Gene:UIP4(YPL186C)|FD-Score:-3.19|P-value:7.07E-4||SGD DESC:Protein that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates; detected in a phosphorylated state in the mitochondrial outer membrane; also detected in ER and nuclear envelope Gene:UNG1(YML021C)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Uracil-DNA glycosylase, required for repair of uracil in DNA formed by spontaneous cytosine deamination, not required for strand-specific mismatch repair, cell-cycle regulated, expressed in late G1, localizes to mitochondria and nucleus Gene:YBL062W(YBL062W_d)|FD-Score:4.64|P-value:1.74E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR238C(YBR238C)|FD-Score:-3.67|P-value:1.21E-4||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YBR242W(YBR242W_p)|FD-Score:-3.21|P-value:6.70E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene Gene:YDL186W(YDL186W_p)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Putative protein of unknown function; YDL186W is not an essential gene Gene:YER135C(YER135C_d)|FD-Score:-3.15|P-value:8.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; YER135C is not an essential gene Gene:YGR237C(YGR237C_p)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YGR273C(YGR273C_p)|FD-Score:4.28|P-value:9.41E-6||SGD DESC:Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene Gene:YIL025C(YIL025C_d)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL108W(YIL108W_p)|FD-Score:4.34|P-value:7.18E-6||SGD DESC:Putative metalloendopeptidase; forms cytoplasmic foci upon DNA replication stress Gene:YIL141W(YIL141W_d)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL027C(YJL027C_p)|FD-Score:7.36|P-value:9.42E-14||SGD DESC:Putative protein of unknown function Gene:YJL132W(YJL132W_p)|FD-Score:3.15|P-value:8.13E-4||SGD DESC:Putative protein of unknown function; localizes to the membrane fraction; possible Zap1p-regulated target gene induced by zinc deficiency; YJL132W is a non-essential gene Gene:YJR038C(YJR038C_d)|FD-Score:4.22|P-value:1.21E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR296W(YLR296W_d)|FD-Score:-5.06|P-value:2.15E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR406C-A(YLR406C-A_p)|FD-Score:-3.52|P-value:2.19E-4||SGD DESC:Putative protein of unknown function Gene:YMR265C(YMR265C_p)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:Putative protein of unknown function Gene:YNR065C(YNR065C_p)|FD-Score:-4|P-value:3.22E-5||SGD DESC:Protein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene Gene:YOL097W-A(YOL097W-A_p)|FD-Score:-3.37|P-value:3.73E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YOR186W(YOR186W_p)|FD-Score:-3.59|P-value:1.68E-4||SGD DESC:Putative protein of unknown function; proper regulation of expression during heat stress is sphingolipid-dependent; YOR186W has a paralog, YLR297W, that arose from the whole genome duplication Gene:YOR263C(YOR263C_d)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF DES3/YOR264W Gene:YOR316C-A(YOR316C-A_p)|FD-Score:4.73|P-value:1.13E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPL136W(YPL136W_d)|FD-Score:4.34|P-value:7.10E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene GIP3/YPL137C Gene:YPR123C(YPR123C_d)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR413C_d5.921.61E-91.65YDR413C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing
YBR153W4.279.75E-60.21RIB7Diaminohydroxyphoshoribosylaminopyrimidine deaminase; catalyzes the second step of the riboflavin biosynthesis pathway
YJL069C4.062.45E-50.83UTP18Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data
YBR004C3.236.15E-40.06GPI18Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria
YKL099C3.187.46E-40.03UTP11Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit
YAL003W3.148.40E-40.33EFB1Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site
YOR203W_d2.810.002480.04YOR203W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps 5' end of essential DED1 gene required for translation initiation
YIL078W2.780.002760.14THS1Threonyl-tRNA synthetase, essential cytoplasmic protein
YOR260W2.630.004220.02GCD1Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YPL233W2.610.004530.18NSL1Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation
YMR211W2.430.007520.00DML1Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
YER159C2.430.007550.02BUR6Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha
YPR161C2.410.008000.05SGV1Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins
YGR002C2.360.009260.08SWC4Component of the Swr1p complex that incorporates Htz1p into chromatin; component of the NuA4 histone acetyltransferase complex
YPR137W2.280.011400.02RRP9Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL005C9.432.07E-21COG7Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YML071C8.054.25E-16COG8Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YJL027C_p7.369.42E-14YJL027C_pPutative protein of unknown function
YOL060C6.494.34E-11MAM3Protein required for normal mitochondrial morphology, has similarity to hemolysins
YLR384C6.301.51E-10IKI3Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD)
YGL059W5.492.02E-8PKP2Mitochondrial protein kinase; negatively regulates activity of the pyruvate dehydrogenase complex by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp1p and phosphatases Ptc5p and Ptc6p; relocalizes from mitochondrion to cytoplasm upon DNA replication stress
YKL211C5.413.17E-8TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YMR319C5.403.28E-8FET4Low-affinity Fe(II) transporter of the plasma membrane
YOR316C-A_p4.731.13E-6YOR316C-A_pPutative protein of unknown function; identified by fungal homology and RT-PCR
YBL062W_d4.641.74E-6YBL062W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YCR106W4.621.94E-6RDS1Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide
YER052C4.415.19E-6HOM3Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
YCL037C4.395.68E-6SRO9Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication
YPL136W_d4.347.10E-6YPL136W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene GIP3/YPL137C
YIL108W_p4.347.18E-6YIL108W_pPutative metalloendopeptidase; forms cytoplasmic foci upon DNA replication stress

GO enrichment analysis for SGTC_2770
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1624.66E-36SGTC_1078idebenone 36.1 μMMiscellaneous36860.084507Golgi
0.1461.67E-29SGTC_1162k048-0130 20.1 μMChemDiv (Drug-like library)67974060.0512821Golgi
0.1446.49E-29SGTC_2740felodipine 17.1 μMMiscellaneous33330.109756
0.1441.19E-28SGTC_13061185-0270 9.0 μMChemDiv (Drug-like library)15480760.038961Golgi
0.1361.34E-25SGTC_624k048-0052 28.0 μMChemDiv (Drug-like library)68355310.0588235Golgi
0.1314.28E-24SGTC_6320781-2353 4.7 μMChemDiv (Drug-like library)68115150.0434783Golgi
0.1309.96E-24SGTC_2766oltipraz 83.9 μMMiscellaneous473180.0555556heme requiring
0.1292.03E-23SGTC_389tpck 16.7 μMMiscellaneous4396470.0740741
0.1293.41E-23SGTC_2763lisinopril 49.3 μMMiscellaneous53621190.0520833
0.1284.08E-23SGTC_22557941087 197.4 μMChembridge (Fragment library)9355610.0617284
0.1232.41E-21SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.08TSC3-RPN4
0.1193.39E-20SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.0294118
0.1187.12E-20SGTC_980074-0023 107.7 μMChemDiv (Drug-like library)35638210.111111Golgi
0.1164.60E-19SGTC_1080nisoldipine 24.4 μMMiscellaneous44990.103448TSC3-RPN4
0.1166.15E-19SGTC_2231micatex 6.9 μMMiscellaneous207370.0806452

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_5181021-011962.6 μM0.270271097762ChemDiv (Drug-like library)387.384784.60115ERG2
SGTC_9113456-4043236 μM0.236111914281ChemDiv (Drug-like library)280.321143.06312mitochondrial processes
SGTC_215856571233.62 μM0.2343752858827Chembridge (Fragment library)269.29521.81603
SGTC_21846075246200 μM0.2343752267320Chembridge (Fragment library)260.288442.13513
SGTC_7323970-172752.7 μM0.2317071275699ChemDiv (Drug-like library)416.4263.87115
SGTC_2246729350791.56 μM0.230769887656Chembridge (Fragment library)244.245980.99413
SGTC_21405586273200 μM0.229508854421Chembridge (Fragment library)223.65562.1402RSC complex & mRNA processing
SGTC_432NS 161961.3 μM0.2205884552ICCB bioactive library362.2266394.11728
SGTC_3580075-00589.45 μM0.2133331069125ChemDiv (Drug-like library)378.255783.84822
SGTC_21095483026200 μM0.213115759335Chembridge (Fragment library)190.19861.10812RSC complex & mRNA processing