fluoxetine FDA approved compound

N-methyl-3-phenyl-3-[4-(trifluoromethyl)phenoxy]propan-1-amine

A selective serotonin reuptake inhibitor.

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PubChem MeSH terms: Antidepressive Agents, Second-Generation;Serotonin Uptake Inhibitors



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2771
Screen concentration 41.0 μM
Source Miscellaneous
PubChem CID 3386
SMILES CNCCC(C1=CC=CC=C1)OC2=CC=C(C=C2)C(F)(F)F
Standardized SMILES CNCCC(Oc1ccc(cc1)C(F)(F)F)c2ccccc2
Molecular weight 309.3261
ALogP 4.03
H-bond donor count 1
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.7
% growth inhibition (Hom. pool) 1.23


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3386
Download HIP data (tab-delimited text)  (excel)
Gene:CCT5(YJR064W)|FD-Score:3.32|P-value:4.58E-4|Clearance:0.29||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:NAM9(YNL137C)|FD-Score:3.74|P-value:9.28E-5|Clearance:0.29||SGD DESC:Mitochondrial ribosomal component of the small subunit Gene:NDD1(YOR372C)|FD-Score:3.93|P-value:4.20E-5|Clearance:0.29||SGD DESC:Transcriptional activator essential for nuclear division; localized to the nucleus; essential component of the mechanism that activates the expression of a set of late-S-phase-specific genes Gene:ORC4(YPR162C)|FD-Score:-5.03|P-value:2.46E-7|Clearance:0||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:RPS15(YOL040C)|FD-Score:3.24|P-value:5.96E-4|Clearance:0.29||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RRP45(YDR280W)|FD-Score:3.51|P-value:2.24E-4|Clearance:0.29||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:SEC23(YPR181C)|FD-Score:-4.01|P-value:3.09E-5|Clearance:0||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:SSL1(YLR005W)|FD-Score:3.16|P-value:7.99E-4|Clearance:0.29||SGD DESC:Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p Gene:TFC1(YBR123C)|FD-Score:3.9|P-value:4.91E-5|Clearance:0.29||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 Gene:TFG2(YGR005C)|FD-Score:3.42|P-value:3.14E-4|Clearance:0.29||SGD DESC:TFIIF (Transcription Factor II) middle subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP30 Gene:YDR413C(YDR413C_d)|FD-Score:3.27|P-value:5.35E-4|Clearance:0.29||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing Gene:CCT5(YJR064W)|FD-Score:3.32|P-value:4.58E-4|Clearance:0.29||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:NAM9(YNL137C)|FD-Score:3.74|P-value:9.28E-5|Clearance:0.29||SGD DESC:Mitochondrial ribosomal component of the small subunit Gene:NDD1(YOR372C)|FD-Score:3.93|P-value:4.20E-5|Clearance:0.29||SGD DESC:Transcriptional activator essential for nuclear division; localized to the nucleus; essential component of the mechanism that activates the expression of a set of late-S-phase-specific genes Gene:ORC4(YPR162C)|FD-Score:-5.03|P-value:2.46E-7|Clearance:0||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:RPS15(YOL040C)|FD-Score:3.24|P-value:5.96E-4|Clearance:0.29||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19 Gene:RRP45(YDR280W)|FD-Score:3.51|P-value:2.24E-4|Clearance:0.29||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:SEC23(YPR181C)|FD-Score:-4.01|P-value:3.09E-5|Clearance:0||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:SSL1(YLR005W)|FD-Score:3.16|P-value:7.99E-4|Clearance:0.29||SGD DESC:Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p Gene:TFC1(YBR123C)|FD-Score:3.9|P-value:4.91E-5|Clearance:0.29||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 Gene:TFG2(YGR005C)|FD-Score:3.42|P-value:3.14E-4|Clearance:0.29||SGD DESC:TFIIF (Transcription Factor II) middle subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP30 Gene:YDR413C(YDR413C_d)|FD-Score:3.27|P-value:5.35E-4|Clearance:0.29||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3386
Download HOP data (tab-delimited text)  (excel)
Gene:AAT2(YLR027C)|FD-Score:3.75|P-value:9.02E-5||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:ADK2(YER170W)|FD-Score:-3.72|P-value:9.84E-5||SGD DESC:Mitochondrial adenylate kinase, catalyzes the reversible synthesis of GTP and AMP from GDP and ADP; may serve as a back-up for synthesizing GTP or ADP depending on metabolic conditions; 3' sequence of ADK2 varies with strain background Gene:ARE2(YNR019W)|FD-Score:4.25|P-value:1.08E-5||SGD DESC:Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the presence of oxygen; ARE2 has a paralog, ARE1, that arose from the whole genome duplication Gene:ARF2(YDL137W)|FD-Score:-3.37|P-value:3.83E-4||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; ARF2 has a paralog, ARF1, that arose from the whole genome duplication Gene:ARL1(YBR164C)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ASI1(YMR119W)|FD-Score:-3.24|P-value:5.97E-4||SGD DESC:Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; ASI1 has a paralog, ASI3, that arose from the whole genome duplication Gene:ATG5(YPL149W)|FD-Score:6.27|P-value:1.81E-10||SGD DESC:Conserved protein involved in autophagy and the Cvt pathway; undergoes conjugation with Atg12p to form a complex involved in Atg8p lipidation; conjugated Atg12p also forms a complex with Atg16p that is essential for autophagosome formation Gene:BSC4(YNL269W)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Protein of unknown function, ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough is increased upon depletion of Sup35p Gene:BSC6(YOL137W)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Protein of unknown function containing 8 putative transmembrane seqments; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression Gene:CAF40(YNL288W)|FD-Score:3.28|P-value:5.18E-4||SGD DESC:Evolutionarily conserved subunit of the CCR4-NOT complex involved in controlling mRNA initiation, elongation and degradation; binds Cdc39p Gene:CAP2(YIL034C)|FD-Score:6.03|P-value:8.18E-10||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CDC26(YFR036W)|FD-Score:3.1|P-value:9.75E-4||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CEX1(YOR112W)|FD-Score:3.16|P-value:7.92E-4||SGD DESC:Cytoplasmic component of the nuclear aminoacylation-dependent tRNA export pathway; interacts with nuclear pore component Nup116p; copurifies with tRNA export receptors Los1p and Msn5p, as well as eIF-1a and the RAN GTPase Gsp1p Gene:DAL82(YNL314W)|FD-Score:3.78|P-value:7.95E-5||SGD DESC:Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain Gene:DCN1(YLR128W)|FD-Score:-3.82|P-value:6.73E-5||SGD DESC:Scaffold-type E3 ligase required for cullin neddylation and ubiquitin ligase activation; contains a ubiquitin-binding domain (UBA) for ubiquitin and Nedd8 (Rub1p) interaction and a PONY domain involved in cullin binding and neddylation Gene:EAF1(YDR359C)|FD-Score:4.28|P-value:9.50E-6||SGD DESC:Component of the NuA4 histone acetyltransferase complex; acts as a platform for assembly of NuA4 subunits into the native complex; required for initiation of pre-meiotic DNA replication, likely due to its requirement for expression of IME1 Gene:FMO1(YHR176W)|FD-Score:4.61|P-value:1.98E-6||SGD DESC:Flavin-containing monooxygenase, localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins Gene:FMP30(YPL103C)|FD-Score:-4.47|P-value:3.90E-6||SGD DESC:Mitochondrial inner membrane protein with a role in maintaining mitochondrial morphology and normal cardiolipin levels; proposed to be involved in N-acylethanolamine metabolism; related to mammalian N-acylPE-specific phospholipase D Gene:GCR2(YNL199C)|FD-Score:3.22|P-value:6.48E-4||SGD DESC:Transcriptional activator of genes involved in glycolysis; interacts and functions with the DNA-binding protein Gcr1p Gene:GFD1(YMR255W)|FD-Score:3.94|P-value:4.14E-5||SGD DESC:Coiled-coiled protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; protein abundance increases in response to DNA replication stress Gene:GPD1(YDL022W)|FD-Score:-3.65|P-value:1.31E-4||SGD DESC:NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity Gene:GPX1(YKL026C)|FD-Score:-5.65|P-value:8.20E-9||SGD DESC:Phospholipid hydroperoxide glutathione peroxidase induced by glucose starvation that protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress Gene:GRX1(YCL035C)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:Glutathione-dependent disulfide oxidoreductase; hydroperoxide and superoxide-radical responsive, heat-stable, with active site cysteine pair; protects cells from oxidative damage; GRX1 has a paralog, GRX2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:HSL1(YKL101W)|FD-Score:4.1|P-value:2.11E-5||SGD DESC:Nim1p-related protein kinase that regulates the morphogenesis and septin checkpoints; associates with the assembled septin filament; required along with Hsl7p for bud neck recruitment, phosphorylation, and degradation of Swe1p Gene:IKI1(YHR187W)|FD-Score:3.95|P-value:3.93E-5||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin Gene:IMG2(YCR071C)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:IOC3(YFR013W)|FD-Score:3.26|P-value:5.60E-4||SGD DESC:Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication Gene:JHD1(YER051W)|FD-Score:4.19|P-value:1.39E-5||SGD DESC:JmjC domain family histone demethylase specific for H3-K36, similar to proteins found in human, mouse, drosophila, X. laevis, C. elegans, and S. pombe Gene:MEH1(YKR007W)|FD-Score:3.76|P-value:8.64E-5||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; loss results in a defect in vacuolar acidification Gene:MSD1(YPL104W)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene Gene:NPL3(YDR432W)|FD-Score:4.96|P-value:3.61E-7||SGD DESC:RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; has a role in repressing translation initiation by binding eIF4G; required for pre-mRNA splicing; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in the cytoplasm; protein abundance increases in response to DNA replication stress Gene:OYE3(YPL171C)|FD-Score:3.42|P-value:3.10E-4||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN), homologous to Oye2p with different ligand binding and catalytic properties; has potential roles in oxidative stress response and programmed cell death Gene:PAT1(YCR077C)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress Gene:PDH1(YPR002W)|FD-Score:-3.65|P-value:1.33E-4||SGD DESC:Mitochondrial protein that participates in respiration, induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate Gene:PEX28(YHR150W)|FD-Score:3.16|P-value:7.87E-4||SGD DESC:Peroxisomal integral membrane peroxin, involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p Gene:PUS7(YOR243C)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria Gene:RME1(YGR044C)|FD-Score:3.97|P-value:3.66E-5||SGD DESC:Zinc finger protein involved in control of meiosis; prevents meiosis by repressing IME1 expression and promotes mitosis by activating CLN2 expression; directly repressed by a1-alpha2 regulator; mediates cell type control of sporulation; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:RPH1(YER169W)|FD-Score:-3.37|P-value:3.82E-4||SGD DESC:JmjC domain-containing histone demethylase; specifically demethylates H3K36 tri- and dimethyl modification states; associates with actively transcribed (RNA polymerase II) regions in vivo and specifically targets H3K36 in its trimethylation state as its substrate; transcriptional repressor of PHR1; Rph1p phosphorylation during DNA damage is under control of the MEC1-RAD53 pathway; RPH1 has a paralog, GIS1, that arose from the whole genome duplication Gene:RPL40B(YKR094C)|FD-Score:-3.18|P-value:7.27E-4||SGD DESC:Ubiquitin-ribosomal 60S subunit protein L40B fusion protein; cleaved to yield ubiquitin and ribosomal protein L40B; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40B has a paralog, RPL40A, that arose from the whole genome duplication Gene:RTS1(YOR014W)|FD-Score:8.7|P-value:1.72E-18||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:SAP155(YFR040W)|FD-Score:3.75|P-value:8.89E-5||SGD DESC:Protein required for function of the Sit4p protein phosphatase; forms a complex with Sit4p; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p; protein abundance increases in response to DNA replication stress Gene:SIC1(YLR079W)|FD-Score:4.84|P-value:6.45E-7||SGD DESC:Cyclin-dependent kinase inhibitor (CKI); inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1 Gene:SKG6(YHR149C)|FD-Score:5.07|P-value:1.99E-7||SGD DESC:Integral membrane protein that localizes primarily to growing sites such as the bud tip or the cell periphery; potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p Gene:SRV2(YNL138W)|FD-Score:-3.41|P-value:3.27E-4||SGD DESC:CAP (cyclase-associated protein) subunit of adenylyl cyclase complex; N-terminus binds adenylyl cyclase and facilitates activation by RAS; C-terminus binds ADP-actin monomers, facilitating regulation of actin dynamics and cell morphogenesis Gene:SSN8(YNL025C)|FD-Score:4.7|P-value:1.32E-6||SGD DESC:Cyclin-like component of the RNA polymerase II holoenzyme, involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression and telomere maintenance Gene:STB4(YMR019W)|FD-Score:5.74|P-value:4.79E-9||SGD DESC:Putative transcription factor; contains a Zn(II)2Cys6 zinc finger domain characteristic of DNA-binding proteins; computational analysis suggests a role in regulation of expression of genes encoding transporters; binds Sin3p in a two-hybrid assay; Gene:TDA11(YHR159W_p)|FD-Score:3.2|P-value:6.98E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele Gene:TFB6(YOR352W)|FD-Score:-4.06|P-value:2.41E-5||SGD DESC:Subunit of TFIIH complex; facilities dissociation of the Ssl2p helices from TFIIH; expression levels regulated by Arg5,6p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:UBC7(YMR022W)|FD-Score:-3.59|P-value:1.66E-4||SGD DESC:Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly Gene:VPS1(YKR001C)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:YBR027C(YBR027C_d)|FD-Score:-3.27|P-value:5.44E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR196C-B(YBR196C-B_p)|FD-Score:4.13|P-value:1.79E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YCL075W(YCL075W)|FD-Score:4.18|P-value:1.48E-5||SGD DESC:Pseudogene: encodes fragment of Ty Pol protein Gene:YDR535C(YDR535C_d)|FD-Score:-3.15|P-value:8.12E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene. Gene:YEL067C(YEL067C_p)|FD-Score:3.72|P-value:9.87E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YGL214W(YGL214W_d)|FD-Score:3.85|P-value:5.86E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion mutation phenotype is likely due to the overlapping verified ORF SKI8/YGL213C Gene:YGR125W(YGR125W_p)|FD-Score:3.4|P-value:3.41E-4||SGD DESC:Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YIL168W(YIL168W)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other <i>S. cerevisiae</i> strain backgrounds YIL168W and adjacent ORF, YIL167W, constitute a single ORF encoding L-serine dehydratase Gene:YJL181W(YJL181W_p)|FD-Score:-3.17|P-value:7.72E-4||SGD DESC:Putative protein of unknown function; expression is cell-cycle regulated as shown by microarray analysis; potential regulatory target of Mbp1p, which binds to the YJL181W promoter region; YJL181W has a paralog, YJR030C, that arose from the whole genome duplication Gene:YKL096C-B(YKL096C-B_p)|FD-Score:7.25|P-value:2.14E-13||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YLR253W(YLR253W_p)|FD-Score:4.97|P-value:3.30E-7||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; transcription is periodic during the metabolic cycle; YLR253W is not an essential gene Gene:YLR279W(YLR279W_d)|FD-Score:3.21|P-value:6.54E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL211C(YNL211C_p)|FD-Score:3.16|P-value:7.89E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL211C is not an essential gene Gene:YNR062C(YNR062C_p)|FD-Score:3.97|P-value:3.57E-5||SGD DESC:Putative membrane protein of unknown function Gene:YOL037C(YOL037C_d)|FD-Score:4.05|P-value:2.57E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOL036W Gene:YPL068C(YPL068C_p)|FD-Score:-3.11|P-value:9.49E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YPR127W(YPR127W)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Putative pyridoxine 4-dehydrogenase; differentially expressed during alcoholic fermentation; expression activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:AAT2(YLR027C)|FD-Score:3.75|P-value:9.02E-5||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:ADK2(YER170W)|FD-Score:-3.72|P-value:9.84E-5||SGD DESC:Mitochondrial adenylate kinase, catalyzes the reversible synthesis of GTP and AMP from GDP and ADP; may serve as a back-up for synthesizing GTP or ADP depending on metabolic conditions; 3' sequence of ADK2 varies with strain background Gene:ARE2(YNR019W)|FD-Score:4.25|P-value:1.08E-5||SGD DESC:Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the presence of oxygen; ARE2 has a paralog, ARE1, that arose from the whole genome duplication Gene:ARF2(YDL137W)|FD-Score:-3.37|P-value:3.83E-4||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; ARF2 has a paralog, ARF1, that arose from the whole genome duplication Gene:ARL1(YBR164C)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ASI1(YMR119W)|FD-Score:-3.24|P-value:5.97E-4||SGD DESC:Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; ASI1 has a paralog, ASI3, that arose from the whole genome duplication Gene:ATG5(YPL149W)|FD-Score:6.27|P-value:1.81E-10||SGD DESC:Conserved protein involved in autophagy and the Cvt pathway; undergoes conjugation with Atg12p to form a complex involved in Atg8p lipidation; conjugated Atg12p also forms a complex with Atg16p that is essential for autophagosome formation Gene:BSC4(YNL269W)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Protein of unknown function, ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough is increased upon depletion of Sup35p Gene:BSC6(YOL137W)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Protein of unknown function containing 8 putative transmembrane seqments; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression Gene:CAF40(YNL288W)|FD-Score:3.28|P-value:5.18E-4||SGD DESC:Evolutionarily conserved subunit of the CCR4-NOT complex involved in controlling mRNA initiation, elongation and degradation; binds Cdc39p Gene:CAP2(YIL034C)|FD-Score:6.03|P-value:8.18E-10||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CDC26(YFR036W)|FD-Score:3.1|P-value:9.75E-4||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CEX1(YOR112W)|FD-Score:3.16|P-value:7.92E-4||SGD DESC:Cytoplasmic component of the nuclear aminoacylation-dependent tRNA export pathway; interacts with nuclear pore component Nup116p; copurifies with tRNA export receptors Los1p and Msn5p, as well as eIF-1a and the RAN GTPase Gsp1p Gene:DAL82(YNL314W)|FD-Score:3.78|P-value:7.95E-5||SGD DESC:Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain Gene:DCN1(YLR128W)|FD-Score:-3.82|P-value:6.73E-5||SGD DESC:Scaffold-type E3 ligase required for cullin neddylation and ubiquitin ligase activation; contains a ubiquitin-binding domain (UBA) for ubiquitin and Nedd8 (Rub1p) interaction and a PONY domain involved in cullin binding and neddylation Gene:EAF1(YDR359C)|FD-Score:4.28|P-value:9.50E-6||SGD DESC:Component of the NuA4 histone acetyltransferase complex; acts as a platform for assembly of NuA4 subunits into the native complex; required for initiation of pre-meiotic DNA replication, likely due to its requirement for expression of IME1 Gene:FMO1(YHR176W)|FD-Score:4.61|P-value:1.98E-6||SGD DESC:Flavin-containing monooxygenase, localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins Gene:FMP30(YPL103C)|FD-Score:-4.47|P-value:3.90E-6||SGD DESC:Mitochondrial inner membrane protein with a role in maintaining mitochondrial morphology and normal cardiolipin levels; proposed to be involved in N-acylethanolamine metabolism; related to mammalian N-acylPE-specific phospholipase D Gene:GCR2(YNL199C)|FD-Score:3.22|P-value:6.48E-4||SGD DESC:Transcriptional activator of genes involved in glycolysis; interacts and functions with the DNA-binding protein Gcr1p Gene:GFD1(YMR255W)|FD-Score:3.94|P-value:4.14E-5||SGD DESC:Coiled-coiled protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; protein abundance increases in response to DNA replication stress Gene:GPD1(YDL022W)|FD-Score:-3.65|P-value:1.31E-4||SGD DESC:NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity Gene:GPX1(YKL026C)|FD-Score:-5.65|P-value:8.20E-9||SGD DESC:Phospholipid hydroperoxide glutathione peroxidase induced by glucose starvation that protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress Gene:GRX1(YCL035C)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:Glutathione-dependent disulfide oxidoreductase; hydroperoxide and superoxide-radical responsive, heat-stable, with active site cysteine pair; protects cells from oxidative damage; GRX1 has a paralog, GRX2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:HSL1(YKL101W)|FD-Score:4.1|P-value:2.11E-5||SGD DESC:Nim1p-related protein kinase that regulates the morphogenesis and septin checkpoints; associates with the assembled septin filament; required along with Hsl7p for bud neck recruitment, phosphorylation, and degradation of Swe1p Gene:IKI1(YHR187W)|FD-Score:3.95|P-value:3.93E-5||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin Gene:IMG2(YCR071C)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:IOC3(YFR013W)|FD-Score:3.26|P-value:5.60E-4||SGD DESC:Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication Gene:JHD1(YER051W)|FD-Score:4.19|P-value:1.39E-5||SGD DESC:JmjC domain family histone demethylase specific for H3-K36, similar to proteins found in human, mouse, drosophila, X. laevis, C. elegans, and S. pombe Gene:MEH1(YKR007W)|FD-Score:3.76|P-value:8.64E-5||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; loss results in a defect in vacuolar acidification Gene:MSD1(YPL104W)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene Gene:NPL3(YDR432W)|FD-Score:4.96|P-value:3.61E-7||SGD DESC:RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; has a role in repressing translation initiation by binding eIF4G; required for pre-mRNA splicing; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in the cytoplasm; protein abundance increases in response to DNA replication stress Gene:OYE3(YPL171C)|FD-Score:3.42|P-value:3.10E-4||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN), homologous to Oye2p with different ligand binding and catalytic properties; has potential roles in oxidative stress response and programmed cell death Gene:PAT1(YCR077C)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress Gene:PDH1(YPR002W)|FD-Score:-3.65|P-value:1.33E-4||SGD DESC:Mitochondrial protein that participates in respiration, induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate Gene:PEX28(YHR150W)|FD-Score:3.16|P-value:7.87E-4||SGD DESC:Peroxisomal integral membrane peroxin, involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p Gene:PUS7(YOR243C)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria Gene:RME1(YGR044C)|FD-Score:3.97|P-value:3.66E-5||SGD DESC:Zinc finger protein involved in control of meiosis; prevents meiosis by repressing IME1 expression and promotes mitosis by activating CLN2 expression; directly repressed by a1-alpha2 regulator; mediates cell type control of sporulation; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:RPH1(YER169W)|FD-Score:-3.37|P-value:3.82E-4||SGD DESC:JmjC domain-containing histone demethylase; specifically demethylates H3K36 tri- and dimethyl modification states; associates with actively transcribed (RNA polymerase II) regions in vivo and specifically targets H3K36 in its trimethylation state as its substrate; transcriptional repressor of PHR1; Rph1p phosphorylation during DNA damage is under control of the MEC1-RAD53 pathway; RPH1 has a paralog, GIS1, that arose from the whole genome duplication Gene:RPL40B(YKR094C)|FD-Score:-3.18|P-value:7.27E-4||SGD DESC:Ubiquitin-ribosomal 60S subunit protein L40B fusion protein; cleaved to yield ubiquitin and ribosomal protein L40B; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40B has a paralog, RPL40A, that arose from the whole genome duplication Gene:RTS1(YOR014W)|FD-Score:8.7|P-value:1.72E-18||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:SAP155(YFR040W)|FD-Score:3.75|P-value:8.89E-5||SGD DESC:Protein required for function of the Sit4p protein phosphatase; forms a complex with Sit4p; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p; protein abundance increases in response to DNA replication stress Gene:SIC1(YLR079W)|FD-Score:4.84|P-value:6.45E-7||SGD DESC:Cyclin-dependent kinase inhibitor (CKI); inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1 Gene:SKG6(YHR149C)|FD-Score:5.07|P-value:1.99E-7||SGD DESC:Integral membrane protein that localizes primarily to growing sites such as the bud tip or the cell periphery; potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p Gene:SRV2(YNL138W)|FD-Score:-3.41|P-value:3.27E-4||SGD DESC:CAP (cyclase-associated protein) subunit of adenylyl cyclase complex; N-terminus binds adenylyl cyclase and facilitates activation by RAS; C-terminus binds ADP-actin monomers, facilitating regulation of actin dynamics and cell morphogenesis Gene:SSN8(YNL025C)|FD-Score:4.7|P-value:1.32E-6||SGD DESC:Cyclin-like component of the RNA polymerase II holoenzyme, involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression and telomere maintenance Gene:STB4(YMR019W)|FD-Score:5.74|P-value:4.79E-9||SGD DESC:Putative transcription factor; contains a Zn(II)2Cys6 zinc finger domain characteristic of DNA-binding proteins; computational analysis suggests a role in regulation of expression of genes encoding transporters; binds Sin3p in a two-hybrid assay; Gene:TDA11(YHR159W_p)|FD-Score:3.2|P-value:6.98E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele Gene:TFB6(YOR352W)|FD-Score:-4.06|P-value:2.41E-5||SGD DESC:Subunit of TFIIH complex; facilities dissociation of the Ssl2p helices from TFIIH; expression levels regulated by Arg5,6p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:UBC7(YMR022W)|FD-Score:-3.59|P-value:1.66E-4||SGD DESC:Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly Gene:VPS1(YKR001C)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:YBR027C(YBR027C_d)|FD-Score:-3.27|P-value:5.44E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR196C-B(YBR196C-B_p)|FD-Score:4.13|P-value:1.79E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YCL075W(YCL075W)|FD-Score:4.18|P-value:1.48E-5||SGD DESC:Pseudogene: encodes fragment of Ty Pol protein Gene:YDR535C(YDR535C_d)|FD-Score:-3.15|P-value:8.12E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene. Gene:YEL067C(YEL067C_p)|FD-Score:3.72|P-value:9.87E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YGL214W(YGL214W_d)|FD-Score:3.85|P-value:5.86E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion mutation phenotype is likely due to the overlapping verified ORF SKI8/YGL213C Gene:YGR125W(YGR125W_p)|FD-Score:3.4|P-value:3.41E-4||SGD DESC:Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YIL168W(YIL168W)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other <i>S. cerevisiae</i> strain backgrounds YIL168W and adjacent ORF, YIL167W, constitute a single ORF encoding L-serine dehydratase Gene:YJL181W(YJL181W_p)|FD-Score:-3.17|P-value:7.72E-4||SGD DESC:Putative protein of unknown function; expression is cell-cycle regulated as shown by microarray analysis; potential regulatory target of Mbp1p, which binds to the YJL181W promoter region; YJL181W has a paralog, YJR030C, that arose from the whole genome duplication Gene:YKL096C-B(YKL096C-B_p)|FD-Score:7.25|P-value:2.14E-13||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YLR253W(YLR253W_p)|FD-Score:4.97|P-value:3.30E-7||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; transcription is periodic during the metabolic cycle; YLR253W is not an essential gene Gene:YLR279W(YLR279W_d)|FD-Score:3.21|P-value:6.54E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL211C(YNL211C_p)|FD-Score:3.16|P-value:7.89E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL211C is not an essential gene Gene:YNR062C(YNR062C_p)|FD-Score:3.97|P-value:3.57E-5||SGD DESC:Putative membrane protein of unknown function Gene:YOL037C(YOL037C_d)|FD-Score:4.05|P-value:2.57E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOL036W Gene:YPL068C(YPL068C_p)|FD-Score:-3.11|P-value:9.49E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YPR127W(YPR127W)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Putative pyridoxine 4-dehydrogenase; differentially expressed during alcoholic fermentation; expression activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR372C3.934.20E-50.29NDD1Transcriptional activator essential for nuclear division; localized to the nucleus; essential component of the mechanism that activates the expression of a set of late-S-phase-specific genes
YBR123C3.904.91E-50.29TFC1One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63
YNL137C3.749.28E-50.29NAM9Mitochondrial ribosomal component of the small subunit
YDR280W3.512.24E-40.29RRP45Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress
YGR005C3.423.14E-40.29TFG2TFIIF (Transcription Factor II) middle subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP30
YJR064W3.324.58E-40.29CCT5Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YDR413C_d3.275.35E-40.29YDR413C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing
YOL040C3.245.96E-40.29RPS15Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15 and bacterial S19
YLR005W3.167.99E-40.29SSL1Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p
YPR034W3.090.001010.29ARP7Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YNL132W2.800.002580.07KRE33Essential protein, required for biogenesis of the small ribosomal subunit; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance
YJL111W2.730.003180.03CCT7Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance
YBR055C2.700.003450.01PRP6Splicing factor, component of the U4/U6-U5 snRNP complex
YBR060C2.700.003500.01ORC2Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; interacts with Spp1p and with trimethylated histone H3; phosphorylated by Cdc28p
YKR083C2.690.003610.01DAD2Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOR014W8.701.72E-18RTS1B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A
YKL096C-B_p7.252.14E-13YKL096C-B_pPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
YPL149W6.271.81E-10ATG5Conserved protein involved in autophagy and the Cvt pathway; undergoes conjugation with Atg12p to form a complex involved in Atg8p lipidation; conjugated Atg12p also forms a complex with Atg16p that is essential for autophagosome formation
YIL034C6.038.18E-10CAP2Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress
YMR019W5.744.79E-9STB4Putative transcription factor; contains a Zn(II)2Cys6 zinc finger domain characteristic of DNA-binding proteins; computational analysis suggests a role in regulation of expression of genes encoding transporters; binds Sin3p in a two-hybrid assay;
YHR149C5.071.99E-7SKG6Integral membrane protein that localizes primarily to growing sites such as the bud tip or the cell periphery; potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p
YLR253W_p4.973.30E-7YLR253W_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; transcription is periodic during the metabolic cycle; YLR253W is not an essential gene
YDR432W4.963.61E-7NPL3RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; has a role in repressing translation initiation by binding eIF4G; required for pre-mRNA splicing; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in the cytoplasm; protein abundance increases in response to DNA replication stress
YLR079W4.846.45E-7SIC1Cyclin-dependent kinase inhibitor (CKI); inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1
YNL025C4.701.32E-6SSN8Cyclin-like component of the RNA polymerase II holoenzyme, involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression and telomere maintenance
YHR176W4.611.98E-6FMO1Flavin-containing monooxygenase, localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins
YDR359C4.289.50E-6EAF1Component of the NuA4 histone acetyltransferase complex; acts as a platform for assembly of NuA4 subunits into the native complex; required for initiation of pre-meiotic DNA replication, likely due to its requirement for expression of IME1
YNR019W4.251.08E-5ARE2Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the presence of oxygen; ARE2 has a paralog, ARE1, that arose from the whole genome duplication
YER051W4.191.39E-5JHD1JmjC domain family histone demethylase specific for H3-K36, similar to proteins found in human, mouse, drosophila, X. laevis, C. elegans, and S. pombe
YCL075W4.181.48E-5YCL075WPseudogene: encodes fragment of Ty Pol protein

GO enrichment analysis for SGTC_2771
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0943.97E-13SGTC_2772vinpocetine 57.1 μMMiscellaneous4439550.0952381
0.0691.37E-7SGTC_2405hr heat shock (37°C) + Streptovitacin 300.0 μMMiscellaneous914670.0571429heat shock/prefoldin
0.0681.64E-7SGTC_2435hr heat shock (37°C) + splitomicin 1.0 μMMiscellaneous52690.0847458heat shock/prefoldin
0.0663.61E-7SGTC_2425hr heat shock (37°C) + 5-fluorouracil 4.6 μMMiscellaneous33850.0576923heat shock/prefoldin
0.0603.40E-6SGTC_28899059656 52.0 μMChembridge (Drug-like library)39498380.0967742
0.0587.21E-6SGTC_20265148700 152.0 μMChembridge (Fragment library)798380.0483871
0.0552.12E-5SGTC_1558st069300 45.6 μMTimTec (Pure natural product library)242080000.0634921
0.0552.40E-5SGTC_2415hr heat shock (37°C) + cantharidin 155.4 μMICCB bioactive library25450.0363636cell wall signaling
0.0552.63E-5SGTC_9031134-0042 114.0 μMChemDiv (Drug-like library)7010090.125
0.0525.45E-5SGTC_1602st001878 87.6 μMTimTec (Natural product derivative library)5954050.0597015
0.0526.55E-5SGTC_310508-1974 57.2 μMChemDiv (Drug-like library)68105250.08
0.0517.85E-5SGTC_2115tiliquinol 12.8 μMChembridge (Fragment library)712080.0892857
0.0501.29E-4SGTC_1841st054148 70.0 μMTimTec (Natural product derivative library)6754420.112676
0.0482.02E-4SGTC_277nystatin 15.5 μMMiscellaneous114687070.0366972
0.0482.03E-4SGTC_1943st076331 65.2 μMTimTec (Natural product derivative library)242084150.0958904

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1094duloxetine25.4 μM0.33333360834NIH Clinical Collection333.87554.22823RPP1 & pyrimidine depletion
SGTC_1103duloxetine36.1 μM0.33333360835NIH Clinical Collection297.414563.84613
SGTC_224benfluorex43.88 μM0.281252318Miscellaneous351.362814.51216
SGTC_2831799903619.48 μM0.2786892988270Chembridge (Drug-like library)323.309654.03115
SGTC_7431275-0778158 μM0.2592592849257ChemDiv (Drug-like library)317.445724.10213excess fatty acid
SGTC_14974476-297841.3 μM0.2586211528379ChemDiv (Drug-like library)331.310193.24317
SGTC_1680diphenhydramine100 μM0.2448988980NIH Clinical Collection291.815683.76612NEO1
SGTC_70192-15824.23 μM0.236364252616ChemDiv (Drug-like library)398.089164.78702
SGTC_3260913710949.47 μM0.23214317478346Chembridge (Drug-like library)255.311683.1561260S ribosome export
SGTC_3221912820949.47 μM0.23076916460723Chembridge (Drug-like library)334.295833.90507
SGTC_1771327-00688.36 μM0.22413815389900ChemDiv (Drug-like library)337.197264.68103