tamoxifen FDA approved compound

2-[4-[(Z)-1,2-diphenylbut-1-enyl]phenoxy]-N,N-dimethylethanamine

Used for treating and preventing breast cancer, tamoxifen competitively inhibits the binding of estradiol to estrogen receptors, thereby preventing the receptor from binding to the estrogen-response element on DNA. The result is a reduction in DNA synthesis and cellular response to estrogen. 

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2774
Screen concentration 10.9 μM
Source Miscellaneous
PubChem CID 2733526
SMILES CCC(=C(C1=CC=CC=C1)C2=CC=C(C=C2)OCCN(C)C)C3=CC=CC=C3
Standardized SMILES CCC(=C(c1ccccc1)c2ccc(OCCN(C)C)cc2)c3ccccc3
Molecular weight 371.5146
ALogP 6.32
H-bond donor count 0
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 7.35
% growth inhibition (Hom. pool) 5.15


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2733526
Download HIP data (tab-delimited text)  (excel)
Gene:BRN1(YBL097W)|FD-Score:3.91|P-value:4.58E-5|Clearance:0.47||SGD DESC:Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission Gene:HYP2(YEL034W)|FD-Score:-3.58|P-value:1.71E-4|Clearance:0||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:LIP1(YMR298W)|FD-Score:4.16|P-value:1.62E-5|Clearance:0.23||SGD DESC:Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis Gene:LSG1(YGL099W)|FD-Score:8.22|P-value:1.04E-16|Clearance:2.19||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:NEO1(YIL048W)|FD-Score:3.92|P-value:4.34E-5|Clearance:0.01||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:NMD3(YHR170W)|FD-Score:5.15|P-value:1.28E-7|Clearance:0.15||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:PAP1(YKR002W)|FD-Score:-3.94|P-value:4.02E-5|Clearance:0||SGD DESC:Poly(A) polymerase, one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs; required for gene looping Gene:POL30(YBR088C)|FD-Score:4.57|P-value:2.43E-6|Clearance:0.42||SGD DESC:Proliferating cell nuclear antigen (PCNA), functions as the sliding clamp for DNA polymerase delta; may function as a docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair Gene:PRP9(YDL030W)|FD-Score:5.01|P-value:2.79E-7|Clearance:0.43||SGD DESC:Subunit of the SF3a splicing factor complex, required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex Gene:RPL25(YOL127W)|FD-Score:-4.52|P-value:3.14E-6|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPP1(YHR062C)|FD-Score:3.31|P-value:4.74E-4|Clearance:0.3||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SEC11(YIR022W)|FD-Score:3.35|P-value:4.09E-4|Clearance:0.04||SGD DESC:18kDa catalytic subunit of the Signal Peptidase Complex (SPC; Spc1p, Spc2p, Spc3p, and Sec11p) which cleaves the signal sequence of proteins targeted to the endoplasmic reticulum Gene:SEC31(YDL195W)|FD-Score:-3.13|P-value:8.86E-4|Clearance:0||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:TFC4(YGR047C)|FD-Score:6.03|P-value:8.26E-10|Clearance:0.88||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA domain of TFIIIC that binds BoxA DNA promoter sites of tRNA and similar genes; has TPR motifs; human homolog is TFIIIC-102 Gene:UTP18(YJL069C)|FD-Score:-3.16|P-value:8.02E-4|Clearance:0||SGD DESC:Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data Gene:YDR053W(YDR053W_d)|FD-Score:3.44|P-value:2.93E-4|Clearance:0.09||SGD DESC:Putative protein of unknown function; open reading frame overlaps 5' end of essential DBF4 gene encoding the regulatory subunit of the Cdc7p-Dbf4p kinase complex Gene:BRN1(YBL097W)|FD-Score:3.91|P-value:4.58E-5|Clearance:0.47||SGD DESC:Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission Gene:HYP2(YEL034W)|FD-Score:-3.58|P-value:1.71E-4|Clearance:0||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:LIP1(YMR298W)|FD-Score:4.16|P-value:1.62E-5|Clearance:0.23||SGD DESC:Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis Gene:LSG1(YGL099W)|FD-Score:8.22|P-value:1.04E-16|Clearance:2.19||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:NEO1(YIL048W)|FD-Score:3.92|P-value:4.34E-5|Clearance:0.01||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:NMD3(YHR170W)|FD-Score:5.15|P-value:1.28E-7|Clearance:0.15||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:PAP1(YKR002W)|FD-Score:-3.94|P-value:4.02E-5|Clearance:0||SGD DESC:Poly(A) polymerase, one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs; required for gene looping Gene:POL30(YBR088C)|FD-Score:4.57|P-value:2.43E-6|Clearance:0.42||SGD DESC:Proliferating cell nuclear antigen (PCNA), functions as the sliding clamp for DNA polymerase delta; may function as a docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair Gene:PRP9(YDL030W)|FD-Score:5.01|P-value:2.79E-7|Clearance:0.43||SGD DESC:Subunit of the SF3a splicing factor complex, required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex Gene:RPL25(YOL127W)|FD-Score:-4.52|P-value:3.14E-6|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPP1(YHR062C)|FD-Score:3.31|P-value:4.74E-4|Clearance:0.3||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SEC11(YIR022W)|FD-Score:3.35|P-value:4.09E-4|Clearance:0.04||SGD DESC:18kDa catalytic subunit of the Signal Peptidase Complex (SPC; Spc1p, Spc2p, Spc3p, and Sec11p) which cleaves the signal sequence of proteins targeted to the endoplasmic reticulum Gene:SEC31(YDL195W)|FD-Score:-3.13|P-value:8.86E-4|Clearance:0||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:TFC4(YGR047C)|FD-Score:6.03|P-value:8.26E-10|Clearance:0.88||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA domain of TFIIIC that binds BoxA DNA promoter sites of tRNA and similar genes; has TPR motifs; human homolog is TFIIIC-102 Gene:UTP18(YJL069C)|FD-Score:-3.16|P-value:8.02E-4|Clearance:0||SGD DESC:Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data Gene:YDR053W(YDR053W_d)|FD-Score:3.44|P-value:2.93E-4|Clearance:0.09||SGD DESC:Putative protein of unknown function; open reading frame overlaps 5' end of essential DBF4 gene encoding the regulatory subunit of the Cdc7p-Dbf4p kinase complex

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2733526
Download HOP data (tab-delimited text)  (excel)
Gene:AHA1(YDR214W)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Co-chaperone that binds to Hsp82p and activates its ATPase activity; similar to Hch1p; expression is regulated by stresses such as heat shock; protein abundance increases in response to DNA replication stress Gene:AIM4(YBR194W)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Protein proposed to be associated with the nuclear pore complex; null mutant is viable, displays elevated frequency of mitochondrial genome loss and is sensitive to freeze-thaw stress Gene:AIM6(YDL237W)|FD-Score:-3.15|P-value:8.17E-4||SGD DESC:Putative protein of unknown function, required for respiratory growth; YDL237W is not an essential gene Gene:ARO1(YDR127W)|FD-Score:-3.77|P-value:8.02E-5||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARP5(YNL059C)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:AUS1(YOR011W)|FD-Score:3.33|P-value:4.36E-4||SGD DESC:Plasma membrane sterol transporter of the ATP-binding cassette family; required, along with Pdr11p, for uptake of exogenous sterols and their incorporation into the plasma membrane; activity is stimulated by phosphatidylserine; sterol uptake is required for anaerobic growth because sterol biosynthesis requires oxygen Gene:BDF1(YLR399C)|FD-Score:3.72|P-value:9.83E-5||SGD DESC:Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p Gene:BRE5(YNR051C)|FD-Score:3.23|P-value:6.10E-4||SGD DESC:Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A Gene:CUL3(YGR003W)|FD-Score:-3.52|P-value:2.18E-4||SGD DESC:Ubiquitin-protein ligase, forms a complex with Elc1p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; cullin family member with similarity to Cdc53p and human CUL3 Gene:DAL81(YIR023W)|FD-Score:-5.91|P-value:1.69E-9||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DOA4(YDR069C)|FD-Score:3.33|P-value:4.29E-4||SGD DESC:Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole Gene:FAB1(YFR019W)|FD-Score:4.1|P-value:2.08E-5||SGD DESC:1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis Gene:FMP43(YGR243W)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium; expression regulated by osmotic and alkaline stresses; protein abundance increases in response to DNA replication stress Gene:GAT2(YMR136W)|FD-Score:4.4|P-value:5.32E-6||SGD DESC:Protein containing GATA family zinc finger motifs; similar to Gln3p and Dal80p; expression repressed by leucine Gene:GFD1(YMR255W)|FD-Score:-3.85|P-value:5.81E-5||SGD DESC:Coiled-coiled protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; protein abundance increases in response to DNA replication stress Gene:GPX1(YKL026C)|FD-Score:-4.68|P-value:1.40E-6||SGD DESC:Phospholipid hydroperoxide glutathione peroxidase induced by glucose starvation that protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress Gene:HST1(YOL068C)|FD-Score:5.86|P-value:2.30E-9||SGD DESC:NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance Gene:HTL1(YCR020W-B)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:IMA5(YJL216C)|FD-Score:3.49|P-value:2.46E-4||SGD DESC:Alpha-glucosidase; specificity for isomaltose, maltose, and palatinose, but not alpha-methylglucoside; member of the IMA isomaltase family; not required for isomaltose utilization, but Ima5p overexpression allows the ima1 null mutant to grow on isomaltose Gene:JIP3(YLR331C_d)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2 Gene:LDB7(YBL006C)|FD-Score:-3.18|P-value:7.33E-4||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:MIR1(YJR077C)|FD-Score:4.48|P-value:3.68E-6||SGD DESC:Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Pic2p but more abundant than Pic2p under normal conditions; phosphorylated Gene:MNN1(YER001W)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 family Gene:MTC3(YGL226W)|FD-Score:4.55|P-value:2.72E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1 Gene:NFT1(YKR103W)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds. Gene:NFU1(YKL040C)|FD-Score:-3.38|P-value:3.57E-4||SGD DESC:Protein involved in iron metabolism in mitochondria; similar to NifU, which is a protein required for the maturation of the Fe/S clusters of nitrogenase in nitrogen-fixing bacteria Gene:OXP1(YKL215C)|FD-Score:-3.89|P-value:5.08E-5||SGD DESC:5-oxoprolinase; enzyme is ATP-dependent and functions as a dimer; similar to mouse Oplah gene; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:OXR1(YPL196W)|FD-Score:3.33|P-value:4.33E-4||SGD DESC:Protein of unknown function required for normal levels of resistance to oxidative damage, null mutants are sensitive to hydrogen peroxide; member of a conserved family of proteins found in eukaryotes Gene:PET10(YKR046C)|FD-Score:-3.23|P-value:6.13E-4||SGD DESC:Protein of unknown function that co-purifies with lipid particles; expression pattern suggests a role in respiratory growth; computational analysis of large-scale protein-protein interaction data suggests a role in ATP/ADP exchange Gene:PEX15(YOL044W)|FD-Score:-4.47|P-value:3.98E-6||SGD DESC:Phosphorylated tail-anchored type II integral peroxisomal membrane protein required for peroxisome biogenesis, cells lacking Pex15p mislocalize peroxisomal matrix proteins to cytosol, overexpression results in impaired peroxisome assembly Gene:PLP1(YDR183W)|FD-Score:-3.26|P-value:5.60E-4||SGD DESC:Protein that interacts with CCT (chaperonin containing TCP-1) complex and has a role in actin and tubulin folding; has weak similarity to phosducins, which are G-protein regulators Gene:POA1(YBR022W)|FD-Score:4.62|P-value:1.95E-6||SGD DESC:Phosphatase that is highly specific for ADP-ribose 1''-phosphate, a tRNA splicing metabolite; may have a role in regulation of tRNA splicing Gene:PST1(YDR055W)|FD-Score:-4.57|P-value:2.44E-6||SGD DESC:Cell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1 Gene:RGI1(YER067W)|FD-Score:-3.3|P-value:4.75E-4||SGD DESC:Protein of unknown function; involved in energy metabolism under respiratory conditions; protein abundance is increased upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:RKM2(YDR198C)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:Ribosomal protein lysine methyltransferase, responsible for trimethylation of the lysine residue at position 3 of Rpl12Ap and Rpl12Bp Gene:RPH1(YER169W)|FD-Score:5.71|P-value:5.54E-9||SGD DESC:JmjC domain-containing histone demethylase; specifically demethylates H3K36 tri- and dimethyl modification states; associates with actively transcribed (RNA polymerase II) regions in vivo and specifically targets H3K36 in its trimethylation state as its substrate; transcriptional repressor of PHR1; Rph1p phosphorylation during DNA damage is under control of the MEC1-RAD53 pathway; RPH1 has a paralog, GIS1, that arose from the whole genome duplication Gene:RPS10A(YOR293W)|FD-Score:5.36|P-value:4.21E-8||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10A has a paralog, RPS10B, that arose from the whole genome duplication Gene:RPS16B(YDL083C)|FD-Score:3.86|P-value:5.60E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16B has a paralog, RPS16A, that arose from the whole genome duplication Gene:SFB3(YHR098C)|FD-Score:3.13|P-value:8.86E-4||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SIL1(YOL031C)|FD-Score:3.14|P-value:8.39E-4||SGD DESC:Nucleotide exchange factor for the endoplasmic reticulum (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocation into the ER; homolog of Yarrowia lipolytica SLS1; GrpE-like protein Gene:SKM1(YOL113W)|FD-Score:4.18|P-value:1.45E-5||SGD DESC:Member of the PAK family of serine/threonine protein kinases with similarity to Ste20p and Cla4p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth Gene:SLA1(YBL007C)|FD-Score:4.11|P-value:2.00E-5||SGD DESC:Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains Gene:SRY1(YKL218C)|FD-Score:3.13|P-value:8.65E-4||SGD DESC:3-hydroxyaspartate dehydratase, deaminates L-threo-3-hydroxyaspartate to form oxaloacetate and ammonia; required in the presence of hydroxyaspartate; highly similar to mouse serine racemase (Srr) but has no serine racemase activity Gene:SSK22(YCR073C)|FD-Score:-3.53|P-value:2.10E-4||SGD DESC:MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; functionally redundant with, and homologous to, Ssk2p; interacts with and is activated by Ssk1p; phosphorylates Pbs2p Gene:THP3(YPR045C)|FD-Score:3.83|P-value:6.42E-5||SGD DESC:Protein that forms a complex with Csn12p that is recruited to transcribed genes and may have a role in transcription elongation; possibly involved in splicing based on pre-mRNA accumulation defect for many intron-containing genes Gene:UBC7(YMR022W)|FD-Score:-3.33|P-value:4.29E-4||SGD DESC:Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly Gene:UGA2(YBR006W)|FD-Score:4.93|P-value:4.21E-7||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:VHS3(YOR054C)|FD-Score:-3.29|P-value:5.08E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:YBL062W(YBL062W_d)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR238C(YBR238C)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YDR524W-C(YDR524W-C_p)|FD-Score:-3.3|P-value:4.87E-4||SGD DESC:Putative protein of unknown function; small ORF identified by SAGE; deletion strains are moderately sensitive to the radiomimetic drug bleomycin Gene:YGL242C(YGL242C_p)|FD-Score:3.13|P-value:8.86E-4||SGD DESC:Putative protein of unknown function; deletion mutant is viable Gene:YGR079W(YGR079W_p)|FD-Score:3.42|P-value:3.10E-4||SGD DESC:Putative protein of unknown function; YGR079W is not an essential gene Gene:YHR078W(YHR078W_p)|FD-Score:3.12|P-value:9.15E-4||SGD DESC:High osmolarity-regulated gene of unknown function Gene:YJU3(YKL094W)|FD-Score:3.16|P-value:7.85E-4||SGD DESC:Monoglyceride lipase (MGL), functional ortholog of mammalian MGL, localizes to lipid particles and membranes, also member of the eukaryotic serine hydrolase family Gene:YKL096C-B(YKL096C-B_p)|FD-Score:-6.33|P-value:1.22E-10||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YME2(YMR302C)|FD-Score:6.56|P-value:2.65E-11||SGD DESC:Integral inner mitochondrial membrane protein with a role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases Gene:YML119W(YML119W_p)|FD-Score:-3.29|P-value:5.03E-4||SGD DESC:Putative protein of unknown function; YML119W is not an essential gene; potential Cdc28p substrate Gene:YMR084W(YMR084W_p)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Putative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YNL226W(YNL226W_d)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene JJJ1/YNL227C Gene:YOR325W(YOR325W_d)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF FRT1 Gene:YPR150W(YPR150W_d)|FD-Score:4.92|P-value:4.27E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SUE1/YPR151C Gene:ZAP1(YJL056C)|FD-Score:4.01|P-value:3.01E-5||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains Gene:ZDS2(YML109W)|FD-Score:-3.51|P-value:2.23E-4||SGD DESC:Protein with a role in regulating Swe1p-dependent polarized growth; involved in maintenance of Cdc55p in the cytoplasm where it promotes mitotic entry; interacts with silencing proteins at the telomere; implicated in the mitotic exit network through regulation of Cdc14p localization; ZDS2 has a paralog, ZDS1, that arose from the whole genome duplication Gene:AHA1(YDR214W)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Co-chaperone that binds to Hsp82p and activates its ATPase activity; similar to Hch1p; expression is regulated by stresses such as heat shock; protein abundance increases in response to DNA replication stress Gene:AIM4(YBR194W)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Protein proposed to be associated with the nuclear pore complex; null mutant is viable, displays elevated frequency of mitochondrial genome loss and is sensitive to freeze-thaw stress Gene:AIM6(YDL237W)|FD-Score:-3.15|P-value:8.17E-4||SGD DESC:Putative protein of unknown function, required for respiratory growth; YDL237W is not an essential gene Gene:ARO1(YDR127W)|FD-Score:-3.77|P-value:8.02E-5||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARP5(YNL059C)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:AUS1(YOR011W)|FD-Score:3.33|P-value:4.36E-4||SGD DESC:Plasma membrane sterol transporter of the ATP-binding cassette family; required, along with Pdr11p, for uptake of exogenous sterols and their incorporation into the plasma membrane; activity is stimulated by phosphatidylserine; sterol uptake is required for anaerobic growth because sterol biosynthesis requires oxygen Gene:BDF1(YLR399C)|FD-Score:3.72|P-value:9.83E-5||SGD DESC:Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p Gene:BRE5(YNR051C)|FD-Score:3.23|P-value:6.10E-4||SGD DESC:Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A Gene:CUL3(YGR003W)|FD-Score:-3.52|P-value:2.18E-4||SGD DESC:Ubiquitin-protein ligase, forms a complex with Elc1p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; cullin family member with similarity to Cdc53p and human CUL3 Gene:DAL81(YIR023W)|FD-Score:-5.91|P-value:1.69E-9||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DOA4(YDR069C)|FD-Score:3.33|P-value:4.29E-4||SGD DESC:Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole Gene:FAB1(YFR019W)|FD-Score:4.1|P-value:2.08E-5||SGD DESC:1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis Gene:FMP43(YGR243W)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium; expression regulated by osmotic and alkaline stresses; protein abundance increases in response to DNA replication stress Gene:GAT2(YMR136W)|FD-Score:4.4|P-value:5.32E-6||SGD DESC:Protein containing GATA family zinc finger motifs; similar to Gln3p and Dal80p; expression repressed by leucine Gene:GFD1(YMR255W)|FD-Score:-3.85|P-value:5.81E-5||SGD DESC:Coiled-coiled protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; protein abundance increases in response to DNA replication stress Gene:GPX1(YKL026C)|FD-Score:-4.68|P-value:1.40E-6||SGD DESC:Phospholipid hydroperoxide glutathione peroxidase induced by glucose starvation that protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress Gene:HST1(YOL068C)|FD-Score:5.86|P-value:2.30E-9||SGD DESC:NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance Gene:HTL1(YCR020W-B)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:IMA5(YJL216C)|FD-Score:3.49|P-value:2.46E-4||SGD DESC:Alpha-glucosidase; specificity for isomaltose, maltose, and palatinose, but not alpha-methylglucoside; member of the IMA isomaltase family; not required for isomaltose utilization, but Ima5p overexpression allows the ima1 null mutant to grow on isomaltose Gene:JIP3(YLR331C_d)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2 Gene:LDB7(YBL006C)|FD-Score:-3.18|P-value:7.33E-4||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:MIR1(YJR077C)|FD-Score:4.48|P-value:3.68E-6||SGD DESC:Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Pic2p but more abundant than Pic2p under normal conditions; phosphorylated Gene:MNN1(YER001W)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 family Gene:MTC3(YGL226W)|FD-Score:4.55|P-value:2.72E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1 Gene:NFT1(YKR103W)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds. Gene:NFU1(YKL040C)|FD-Score:-3.38|P-value:3.57E-4||SGD DESC:Protein involved in iron metabolism in mitochondria; similar to NifU, which is a protein required for the maturation of the Fe/S clusters of nitrogenase in nitrogen-fixing bacteria Gene:OXP1(YKL215C)|FD-Score:-3.89|P-value:5.08E-5||SGD DESC:5-oxoprolinase; enzyme is ATP-dependent and functions as a dimer; similar to mouse Oplah gene; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:OXR1(YPL196W)|FD-Score:3.33|P-value:4.33E-4||SGD DESC:Protein of unknown function required for normal levels of resistance to oxidative damage, null mutants are sensitive to hydrogen peroxide; member of a conserved family of proteins found in eukaryotes Gene:PET10(YKR046C)|FD-Score:-3.23|P-value:6.13E-4||SGD DESC:Protein of unknown function that co-purifies with lipid particles; expression pattern suggests a role in respiratory growth; computational analysis of large-scale protein-protein interaction data suggests a role in ATP/ADP exchange Gene:PEX15(YOL044W)|FD-Score:-4.47|P-value:3.98E-6||SGD DESC:Phosphorylated tail-anchored type II integral peroxisomal membrane protein required for peroxisome biogenesis, cells lacking Pex15p mislocalize peroxisomal matrix proteins to cytosol, overexpression results in impaired peroxisome assembly Gene:PLP1(YDR183W)|FD-Score:-3.26|P-value:5.60E-4||SGD DESC:Protein that interacts with CCT (chaperonin containing TCP-1) complex and has a role in actin and tubulin folding; has weak similarity to phosducins, which are G-protein regulators Gene:POA1(YBR022W)|FD-Score:4.62|P-value:1.95E-6||SGD DESC:Phosphatase that is highly specific for ADP-ribose 1''-phosphate, a tRNA splicing metabolite; may have a role in regulation of tRNA splicing Gene:PST1(YDR055W)|FD-Score:-4.57|P-value:2.44E-6||SGD DESC:Cell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1 Gene:RGI1(YER067W)|FD-Score:-3.3|P-value:4.75E-4||SGD DESC:Protein of unknown function; involved in energy metabolism under respiratory conditions; protein abundance is increased upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:RKM2(YDR198C)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:Ribosomal protein lysine methyltransferase, responsible for trimethylation of the lysine residue at position 3 of Rpl12Ap and Rpl12Bp Gene:RPH1(YER169W)|FD-Score:5.71|P-value:5.54E-9||SGD DESC:JmjC domain-containing histone demethylase; specifically demethylates H3K36 tri- and dimethyl modification states; associates with actively transcribed (RNA polymerase II) regions in vivo and specifically targets H3K36 in its trimethylation state as its substrate; transcriptional repressor of PHR1; Rph1p phosphorylation during DNA damage is under control of the MEC1-RAD53 pathway; RPH1 has a paralog, GIS1, that arose from the whole genome duplication Gene:RPS10A(YOR293W)|FD-Score:5.36|P-value:4.21E-8||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10A has a paralog, RPS10B, that arose from the whole genome duplication Gene:RPS16B(YDL083C)|FD-Score:3.86|P-value:5.60E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16B has a paralog, RPS16A, that arose from the whole genome duplication Gene:SFB3(YHR098C)|FD-Score:3.13|P-value:8.86E-4||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SIL1(YOL031C)|FD-Score:3.14|P-value:8.39E-4||SGD DESC:Nucleotide exchange factor for the endoplasmic reticulum (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocation into the ER; homolog of Yarrowia lipolytica SLS1; GrpE-like protein Gene:SKM1(YOL113W)|FD-Score:4.18|P-value:1.45E-5||SGD DESC:Member of the PAK family of serine/threonine protein kinases with similarity to Ste20p and Cla4p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth Gene:SLA1(YBL007C)|FD-Score:4.11|P-value:2.00E-5||SGD DESC:Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains Gene:SRY1(YKL218C)|FD-Score:3.13|P-value:8.65E-4||SGD DESC:3-hydroxyaspartate dehydratase, deaminates L-threo-3-hydroxyaspartate to form oxaloacetate and ammonia; required in the presence of hydroxyaspartate; highly similar to mouse serine racemase (Srr) but has no serine racemase activity Gene:SSK22(YCR073C)|FD-Score:-3.53|P-value:2.10E-4||SGD DESC:MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; functionally redundant with, and homologous to, Ssk2p; interacts with and is activated by Ssk1p; phosphorylates Pbs2p Gene:THP3(YPR045C)|FD-Score:3.83|P-value:6.42E-5||SGD DESC:Protein that forms a complex with Csn12p that is recruited to transcribed genes and may have a role in transcription elongation; possibly involved in splicing based on pre-mRNA accumulation defect for many intron-containing genes Gene:UBC7(YMR022W)|FD-Score:-3.33|P-value:4.29E-4||SGD DESC:Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly Gene:UGA2(YBR006W)|FD-Score:4.93|P-value:4.21E-7||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:VHS3(YOR054C)|FD-Score:-3.29|P-value:5.08E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:YBL062W(YBL062W_d)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR238C(YBR238C)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YDR524W-C(YDR524W-C_p)|FD-Score:-3.3|P-value:4.87E-4||SGD DESC:Putative protein of unknown function; small ORF identified by SAGE; deletion strains are moderately sensitive to the radiomimetic drug bleomycin Gene:YGL242C(YGL242C_p)|FD-Score:3.13|P-value:8.86E-4||SGD DESC:Putative protein of unknown function; deletion mutant is viable Gene:YGR079W(YGR079W_p)|FD-Score:3.42|P-value:3.10E-4||SGD DESC:Putative protein of unknown function; YGR079W is not an essential gene Gene:YHR078W(YHR078W_p)|FD-Score:3.12|P-value:9.15E-4||SGD DESC:High osmolarity-regulated gene of unknown function Gene:YJU3(YKL094W)|FD-Score:3.16|P-value:7.85E-4||SGD DESC:Monoglyceride lipase (MGL), functional ortholog of mammalian MGL, localizes to lipid particles and membranes, also member of the eukaryotic serine hydrolase family Gene:YKL096C-B(YKL096C-B_p)|FD-Score:-6.33|P-value:1.22E-10||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YME2(YMR302C)|FD-Score:6.56|P-value:2.65E-11||SGD DESC:Integral inner mitochondrial membrane protein with a role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases Gene:YML119W(YML119W_p)|FD-Score:-3.29|P-value:5.03E-4||SGD DESC:Putative protein of unknown function; YML119W is not an essential gene; potential Cdc28p substrate Gene:YMR084W(YMR084W_p)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Putative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YNL226W(YNL226W_d)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene JJJ1/YNL227C Gene:YOR325W(YOR325W_d)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF FRT1 Gene:YPR150W(YPR150W_d)|FD-Score:4.92|P-value:4.27E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SUE1/YPR151C Gene:ZAP1(YJL056C)|FD-Score:4.01|P-value:3.01E-5||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains Gene:ZDS2(YML109W)|FD-Score:-3.51|P-value:2.23E-4||SGD DESC:Protein with a role in regulating Swe1p-dependent polarized growth; involved in maintenance of Cdc55p in the cytoplasm where it promotes mitotic entry; interacts with silencing proteins at the telomere; implicated in the mitotic exit network through regulation of Cdc14p localization; ZDS2 has a paralog, ZDS1, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL099W8.221.04E-162.19LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YGR047C6.038.26E-100.88TFC4One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA domain of TFIIIC that binds BoxA DNA promoter sites of tRNA and similar genes; has TPR motifs; human homolog is TFIIIC-102
YHR170W5.151.28E-70.15NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YDL030W5.012.79E-70.43PRP9Subunit of the SF3a splicing factor complex, required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex
YBR088C4.572.43E-60.41POL30Proliferating cell nuclear antigen (PCNA), functions as the sliding clamp for DNA polymerase delta; may function as a docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair
YMR298W4.161.62E-50.23LIP1Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis
YIL048W3.924.34E-50.01NEO1Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus
YBL097W3.914.58E-50.47BRN1Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission
YDR053W_d3.442.93E-40.09YDR053W_dPutative protein of unknown function; open reading frame overlaps 5' end of essential DBF4 gene encoding the regulatory subunit of the Cdc7p-Dbf4p kinase complex
YIR022W3.354.09E-40.04SEC1118kDa catalytic subunit of the Signal Peptidase Complex (SPC; Spc1p, Spc2p, Spc3p, and Sec11p) which cleaves the signal sequence of proteins targeted to the endoplasmic reticulum
YHR062C3.314.74E-40.30RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YDR238C3.000.001340.03SEC26Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP)
YHR083W2.970.001480.25SAM35Essential component of the sorting and assembly machinery (SAM complex or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane
YJL035C2.720.003260.02TAD2Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad3p that converts adenosine to inosine at the wobble position of several tRNAs
YOR204W2.700.003470.06DED1ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR302C6.562.65E-11YME2Integral inner mitochondrial membrane protein with a role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases
YOL068C5.862.30E-9HST1NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance
YER169W5.715.54E-9RPH1JmjC domain-containing histone demethylase; specifically demethylates H3K36 tri- and dimethyl modification states; associates with actively transcribed (RNA polymerase II) regions in vivo and specifically targets H3K36 in its trimethylation state as its substrate; transcriptional repressor of PHR1; Rph1p phosphorylation during DNA damage is under control of the MEC1-RAD53 pathway; RPH1 has a paralog, GIS1, that arose from the whole genome duplication
YOR293W5.364.21E-8RPS10AProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10A has a paralog, RPS10B, that arose from the whole genome duplication
YBR006W4.934.21E-7UGA2Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm
YPR150W_d4.924.27E-7YPR150W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SUE1/YPR151C
YBR022W4.621.95E-6POA1Phosphatase that is highly specific for ADP-ribose 1''-phosphate, a tRNA splicing metabolite; may have a role in regulation of tRNA splicing
YGL226W4.552.72E-6MTC3Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1
YJR077C4.483.68E-6MIR1Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Pic2p but more abundant than Pic2p under normal conditions; phosphorylated
YMR136W4.405.32E-6GAT2Protein containing GATA family zinc finger motifs; similar to Gln3p and Dal80p; expression repressed by leucine
YER001W4.201.32E-5MNN1Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 family
YOL113W4.181.45E-5SKM1Member of the PAK family of serine/threonine protein kinases with similarity to Ste20p and Cla4p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth
YBL007C4.112.00E-5SLA1Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains
YFR019W4.102.08E-5FAB11-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis
YJL056C4.013.01E-5ZAP1Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains

GO enrichment analysis for SGTC_2774
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1771.54E-42SGTC_618k072-0232 33.2 μMChemDiv (Drug-like library)58727560.12307760S ribosome export
0.1661.30E-37SGTC_970535-0701 19.1 μMChemDiv (Drug-like library)28320130.11594260S ribosome export
0.1583.97E-34SGTC_7651319-0117 244.0 μMChemDiv (Drug-like library)34877460.12048260S ribosome export
0.1554.31E-33SGTC_8103825-7150 143.0 μMChemDiv (Drug-like library)223304110.094594660S ribosome export
0.1527.23E-32SGTC_1070indatraline 8.8 μMNIH Clinical Collection103144720.096774260S ribosome export
0.1431.54E-28SGTC_2775cyproheptadine 69.6 μMMiscellaneous29130.107143
0.1411.08E-27SGTC_29379044753 11.3 μMChembridge (Drug-like library)64655300.078947460S ribosome export
0.1382.32E-26SGTC_1775st081588 35.2 μMTimTec (Natural product derivative library)14264430.10389660S ribosome export
0.1293.20E-23SGTC_22067254556 147.0 μMChembridge (Fragment library)43989050.092307760S ribosome export
0.1286.68E-23SGTC_15924',5'-dihydroxyflavone 8.8 μMTimTec (Pure natural product library)6886690.12698460S ribosome export
0.1288.12E-23SGTC_620180-0423 3.2 μMChemDiv (Drug-like library)301020.14925460S ribosome export
0.1278.98E-23SGTC_14924158-0558 28.5 μMChemDiv (Drug-like library)39672680.10666760S ribosome export
0.1263.88E-22SGTC_32769139060 49.5 μMChembridge (Drug-like library)48706190.10256460S ribosome export
0.1226.50E-21SGTC_1612st002233 45.4 μMTimTec (Natural product derivative library)10350210.194444
0.1201.77E-20SGTC_1886st057356 49.9 μMTimTec (Natural product derivative library)242077470.0560S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_270tamoxifen5.46 μM15376Miscellaneous371.514566.31902NEO1
SGTC_1092toremifene6.32 μM0.7380953005573NIH Clinical Collection405.959626.29802
SGTC_2769toremifene3.73 μM0.7380953005573Miscellaneous405.959626.29802
SGTC_2757clomiphene4.22 μM0.5434781548953Miscellaneous405.959626.48302fatty acid desaturase (OLE1)
SGTC_1680diphenhydramine100 μM0.3636368980NIH Clinical Collection291.815683.76612NEO1
SGTC_3260913710949.47 μM0.3617478346Chembridge (Drug-like library)255.311683.1561260S ribosome export
SGTC_2418pentamidine31.67 μM0.3043484735Miscellaneous340.419462.65744mitochondrial processes
SGTC_2640pentamidine10.38 μM0.3043484735Miscellaneous340.419462.65744mitochondrial processes
SGTC_15091326-113180 μM0.297872768313ChemDiv (Drug-like library)239.312283.09402
SGTC_1290088-0017176.64 μM0.296296182446ChemDiv (Drug-like library)278.343483.63914
SGTC_2929901334824.6 μM0.2909092995952Chembridge (Drug-like library)310.175223.99912
SGTC_1738st03555574 μM0.2857145331140TimTec (Natural product derivative library)270.2982034.23903TSC3-RPN4