5215298

2-[5-(4-methoxyphenyl)-4,5-dihydro-1H-pyrazol-3-yl]indene-1,3-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2780
Screen concentration 71.4 μM
Source Chembridge (Drug-like library)
PubChem CID 2834131
SMILES COC1=CC=C(C=C1)C2CC(=NN2)C3C(=O)C4=CC=CC=C4C3=O
Standardized SMILES COc1ccc(cc1)C2CC(=NN2)C3C(=O)c4ccccc4C3=O
Molecular weight 320.3419
ALogP 2.33
H-bond donor count 1
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.62
% growth inhibition (Hom. pool) 1.17


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2834131
Download HIP data (tab-delimited text)  (excel)
Gene:ARC40(YBR234C)|FD-Score:3.46|P-value:2.72E-4|Clearance:0.02||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:CAB4(YGR277C)|FD-Score:3.19|P-value:7.14E-4|Clearance:0.05||SGD DESC:Probable pantetheine-phosphate adenylyltransferase (PPAT), which catalyzes the fourth step in the biosynthesis of coenzyme A from pantothenate; null mutant lethality is complemented by E. coli coaD (encoding PPAT); widely conserved Gene:CCA1(YER168C)|FD-Score:-3.32|P-value:4.51E-4|Clearance:0||SGD DESC:ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites Gene:CCT7(YJL111W)|FD-Score:3.65|P-value:1.32E-4|Clearance:0.14||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:DAD2(YKR083C)|FD-Score:4.7|P-value:1.32E-6|Clearance:0.29||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:FBA1(YKL060C)|FD-Score:3.95|P-value:3.98E-5|Clearance:0.08||SGD DESC:Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversion of fructose 1,6 bisphosphate to glyceraldehyde-3-P and dihydroxyacetone-P; locates to mitochondrial outer surface upon oxidative stress Gene:FCF1(YDR339C)|FD-Score:6.14|P-value:4.08E-10|Clearance:0.22||SGD DESC:Putative PINc domain nuclease required for early cleavages of 35S pre-rRNA and maturation of 18S rRNA; component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; copurifies with Faf1p Gene:GCR1(YPL075W)|FD-Score:5.22|P-value:9.15E-8|Clearance:0.24||SGD DESC:Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p Gene:GPN3(YLR243W_p)|FD-Score:5.91|P-value:1.70E-9|Clearance:0.11||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn2p Gene:HSH49(YOR319W)|FD-Score:3.3|P-value:4.82E-4|Clearance:0.11||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:LAS17(YOR181W)|FD-Score:4.4|P-value:5.34E-6|Clearance:0.13||SGD DESC:Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP) Gene:MCD4(YKL165C)|FD-Score:8.29|P-value:5.79E-17|Clearance:2.15||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:OLE1(YGL055W)|FD-Score:-4.64|P-value:1.77E-6|Clearance:0||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:PGS1(YCL004W)|FD-Score:4.93|P-value:4.20E-7|Clearance:0.23||SGD DESC:Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis Gene:POP3(YNL282W)|FD-Score:3.14|P-value:8.45E-4|Clearance:0.08||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRE8(YML092C)|FD-Score:5.29|P-value:6.04E-8|Clearance:0.08||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:RET2(YFR051C)|FD-Score:3.34|P-value:4.22E-4|Clearance:0.04||SGD DESC:Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RIO1(YOR119C)|FD-Score:-3.18|P-value:7.43E-4|Clearance:0||SGD DESC:Essential serine kinase involved in cell cycle progression and processing of the 20S pre-rRNA into mature 18S rRNA Gene:RPL5(YPL131W)|FD-Score:-3.61|P-value:1.56E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:RRN11(YML043C)|FD-Score:5.8|P-value:3.28E-9|Clearance:0.51||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:RRP4(YHR069C)|FD-Score:8.44|P-value:1.56E-17|Clearance:2.15||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:RRP9(YPR137W)|FD-Score:3.51|P-value:2.23E-4|Clearance:0.03||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:RSE1(YML049C)|FD-Score:-3.47|P-value:2.61E-4|Clearance:0||SGD DESC:Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport Gene:SDH3(YKL141W)|FD-Score:3.44|P-value:2.88E-4|Clearance:0.04||SGD DESC:Subunit of both succinate dehydrogenase and of TIM22 translocase; functions as the cytochrome b subunit of succinate dehydrogenase, which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex Gene:SPC42(YKL042W)|FD-Score:3.4|P-value:3.40E-4|Clearance:0.06||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane Gene:SPP382(YLR424W)|FD-Score:-3.41|P-value:3.28E-4|Clearance:0||SGD DESC:Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation Gene:SPT14(YPL175W)|FD-Score:4.27|P-value:9.80E-6|Clearance:0.32||SGD DESC:UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins Gene:SRB6(YBR253W)|FD-Score:4.97|P-value:3.30E-7|Clearance:0.05||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:SUI3(YPL237W)|FD-Score:3.86|P-value:5.59E-5|Clearance:0.21||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:SWI1(YPL016W)|FD-Score:-3.12|P-value:8.99E-4|Clearance:0||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer Gene:TFB4(YPR056W)|FD-Score:-4|P-value:3.19E-5|Clearance:0||SGD DESC:Subunit of TFIIH complex, involved in transcription initiation, similar to 34 kDa subunit of human TFIIH; interacts with Ssl1p Gene:TFC6(YDR362C)|FD-Score:-3.94|P-value:4.10E-5|Clearance:0||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 Gene:THG1(YGR024C)|FD-Score:3.48|P-value:2.53E-4|Clearance:0.02||SGD DESC:tRNAHis guanylyltransferase, adds a guanosine residue to the 5' end of tRNAHis after transcription and RNase P cleavage; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes Gene:YBR190W(YBR190W_d)|FD-Score:5.92|P-value:1.60E-9|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W Gene:YOR102W(YOR102W_d)|FD-Score:-3.31|P-value:4.72E-4|Clearance:0||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; extensively overlaps essential OST2 gene encoding a subunit of the ER lumen oligosaccharyltransferase complex Gene:ARC40(YBR234C)|FD-Score:3.46|P-value:2.72E-4|Clearance:0.02||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:CAB4(YGR277C)|FD-Score:3.19|P-value:7.14E-4|Clearance:0.05||SGD DESC:Probable pantetheine-phosphate adenylyltransferase (PPAT), which catalyzes the fourth step in the biosynthesis of coenzyme A from pantothenate; null mutant lethality is complemented by E. coli coaD (encoding PPAT); widely conserved Gene:CCA1(YER168C)|FD-Score:-3.32|P-value:4.51E-4|Clearance:0||SGD DESC:ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites Gene:CCT7(YJL111W)|FD-Score:3.65|P-value:1.32E-4|Clearance:0.14||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:DAD2(YKR083C)|FD-Score:4.7|P-value:1.32E-6|Clearance:0.29||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:FBA1(YKL060C)|FD-Score:3.95|P-value:3.98E-5|Clearance:0.08||SGD DESC:Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversion of fructose 1,6 bisphosphate to glyceraldehyde-3-P and dihydroxyacetone-P; locates to mitochondrial outer surface upon oxidative stress Gene:FCF1(YDR339C)|FD-Score:6.14|P-value:4.08E-10|Clearance:0.22||SGD DESC:Putative PINc domain nuclease required for early cleavages of 35S pre-rRNA and maturation of 18S rRNA; component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; copurifies with Faf1p Gene:GCR1(YPL075W)|FD-Score:5.22|P-value:9.15E-8|Clearance:0.24||SGD DESC:Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p Gene:GPN3(YLR243W_p)|FD-Score:5.91|P-value:1.70E-9|Clearance:0.11||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn2p Gene:HSH49(YOR319W)|FD-Score:3.3|P-value:4.82E-4|Clearance:0.11||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:LAS17(YOR181W)|FD-Score:4.4|P-value:5.34E-6|Clearance:0.13||SGD DESC:Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP) Gene:MCD4(YKL165C)|FD-Score:8.29|P-value:5.79E-17|Clearance:2.15||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:OLE1(YGL055W)|FD-Score:-4.64|P-value:1.77E-6|Clearance:0||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:PGS1(YCL004W)|FD-Score:4.93|P-value:4.20E-7|Clearance:0.23||SGD DESC:Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis Gene:POP3(YNL282W)|FD-Score:3.14|P-value:8.45E-4|Clearance:0.08||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRE8(YML092C)|FD-Score:5.29|P-value:6.04E-8|Clearance:0.08||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:RET2(YFR051C)|FD-Score:3.34|P-value:4.22E-4|Clearance:0.04||SGD DESC:Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RIO1(YOR119C)|FD-Score:-3.18|P-value:7.43E-4|Clearance:0||SGD DESC:Essential serine kinase involved in cell cycle progression and processing of the 20S pre-rRNA into mature 18S rRNA Gene:RPL5(YPL131W)|FD-Score:-3.61|P-value:1.56E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:RRN11(YML043C)|FD-Score:5.8|P-value:3.28E-9|Clearance:0.51||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:RRP4(YHR069C)|FD-Score:8.44|P-value:1.56E-17|Clearance:2.15||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:RRP9(YPR137W)|FD-Score:3.51|P-value:2.23E-4|Clearance:0.03||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:RSE1(YML049C)|FD-Score:-3.47|P-value:2.61E-4|Clearance:0||SGD DESC:Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport Gene:SDH3(YKL141W)|FD-Score:3.44|P-value:2.88E-4|Clearance:0.04||SGD DESC:Subunit of both succinate dehydrogenase and of TIM22 translocase; functions as the cytochrome b subunit of succinate dehydrogenase, which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex Gene:SPC42(YKL042W)|FD-Score:3.4|P-value:3.40E-4|Clearance:0.06||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane Gene:SPP382(YLR424W)|FD-Score:-3.41|P-value:3.28E-4|Clearance:0||SGD DESC:Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation Gene:SPT14(YPL175W)|FD-Score:4.27|P-value:9.80E-6|Clearance:0.32||SGD DESC:UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins Gene:SRB6(YBR253W)|FD-Score:4.97|P-value:3.30E-7|Clearance:0.05||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:SUI3(YPL237W)|FD-Score:3.86|P-value:5.59E-5|Clearance:0.21||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:SWI1(YPL016W)|FD-Score:-3.12|P-value:8.99E-4|Clearance:0||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer Gene:TFB4(YPR056W)|FD-Score:-4|P-value:3.19E-5|Clearance:0||SGD DESC:Subunit of TFIIH complex, involved in transcription initiation, similar to 34 kDa subunit of human TFIIH; interacts with Ssl1p Gene:TFC6(YDR362C)|FD-Score:-3.94|P-value:4.10E-5|Clearance:0||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 Gene:THG1(YGR024C)|FD-Score:3.48|P-value:2.53E-4|Clearance:0.02||SGD DESC:tRNAHis guanylyltransferase, adds a guanosine residue to the 5' end of tRNAHis after transcription and RNase P cleavage; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes Gene:YBR190W(YBR190W_d)|FD-Score:5.92|P-value:1.60E-9|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W Gene:YOR102W(YOR102W_d)|FD-Score:-3.31|P-value:4.72E-4|Clearance:0||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; extensively overlaps essential OST2 gene encoding a subunit of the ER lumen oligosaccharyltransferase complex

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2834131
Download HOP data (tab-delimited text)  (excel)
Gene:ABM1(YJR108W)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Protein of unknown function, required for normal microtubule organization Gene:ACE2(YLR131C)|FD-Score:4.07|P-value:2.33E-5||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:AIM10(YER087W)|FD-Score:-7.43|P-value:5.24E-14||SGD DESC:Protein with similarity to tRNA synthetases; non-tagged protein is detected in purified mitochondria; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:ANP1(YEL036C)|FD-Score:-3.35|P-value:3.98E-4||SGD DESC:Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol Gene:APM4(YOL062C)|FD-Score:-5|P-value:2.79E-7||SGD DESC:Mu2-like subunit of the clathrin associated protein complex (AP-2); involved in vesicle transport Gene:ARC18(YLR370C)|FD-Score:3.13|P-value:8.83E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ATG9(YDL149W)|FD-Score:4.08|P-value:2.27E-5||SGD DESC:Transmembrane protein involved in forming Cvt and autophagic vesicles; cycles between the phagophore assembly site (PAS) and other cytosolic punctate structures, not found in autophagosomes; may be involved in membrane delivery to the PAS Gene:ATP19(YOL077W-A)|FD-Score:3.86|P-value:5.72E-5||SGD DESC:Subunit k of the mitochondrial F1F0 ATP synthase, which is a large enzyme complex required for ATP synthesis; associated only with the dimeric form of ATP synthase Gene:AVT5(YBL089W)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Putative transporter; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters; AVT5 has a paralog, AVT6, that arose from the whole genome duplication Gene:BRE4(YDL231C)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:Zinc finger protein containing five transmembrane domains; null mutant exhibits strongly fragmented vacuoles and sensitivity to brefeldin A, a drug which is known to affect intracellular transport Gene:BSP1(YPR171W)|FD-Score:4.49|P-value:3.52E-6||SGD DESC:Adapter that links synaptojanins Inp52p and Inp53p to the cortical actin cytoskeleton Gene:BUD14(YAR014C)|FD-Score:6.42|P-value:6.89E-11||SGD DESC:Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; inhibitor of the actin assembly factor Bnr1p (formin); diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress Gene:BUD21(YOR078W)|FD-Score:-3.48|P-value:2.54E-4||SGD DESC:Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated as bud-site selection mutant that displays a random budding pattern Gene:CEX1(YOR112W)|FD-Score:-3.24|P-value:5.99E-4||SGD DESC:Cytoplasmic component of the nuclear aminoacylation-dependent tRNA export pathway; interacts with nuclear pore component Nup116p; copurifies with tRNA export receptors Los1p and Msn5p, as well as eIF-1a and the RAN GTPase Gsp1p Gene:CHS3(YBR023C)|FD-Score:4.96|P-value:3.48E-7||SGD DESC:Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan Gene:CLC1(YGR167W)|FD-Score:3.12|P-value:9.16E-4||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:COX12(YLR038C)|FD-Score:9.06|P-value:6.60E-20||SGD DESC:Subunit VIb of cytochrome c oxidase; cytochrome c oxidase is also known as respiratory Complex IV and is the terminal member of the mitochondrial inner membrane electron transport chain; required for assembly of cytochrome c oxidase but not required for activity after assembly; phosphorylated; easily released from the intermembrane space, suggesting a loose association with Complex IV Gene:CPR6(YLR216C)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity; protein abundance increases in response to DNA replication stress Gene:CSF1(YLR087C)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CSM3(YMR048W)|FD-Score:5.38|P-value:3.78E-8||SGD DESC:Replication fork associated factor; required for stable replication fork pausing; component of the DNA replication checkpoint pathway; required for accurate chromosome segregation during meiosis; forms nuclear foci upon DNA replication stress Gene:CSR1(YLR380W)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Phosphatidylinositol transfer protein; has a potential role in regulating lipid and fatty acid metabolism under heme-depleted conditions; interacts specifically with thioredoxin peroxidase; may have a role in oxidative stress resistance; protein abundance increases in response to DNA replication stress Gene:CTR1(YPR124W)|FD-Score:-3.96|P-value:3.71E-5||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CUE1(YMR264W)|FD-Score:9.05|P-value:7.47E-20||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:CUP9(YPL177C)|FD-Score:4.81|P-value:7.46E-7||SGD DESC:Homeodomain-containing transcriptional repressor; regulates expression of PTR2, which encodes a major peptide transporter; imported peptides activate ubiquitin-dependent proteolysis, resulting in degradation of Cup9p and de-repression of PTR2 transcription; CUP9 has a paralog, TOS8, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:CWH43(YCR017C)|FD-Score:-3.55|P-value:1.94E-4||SGD DESC:Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion Gene:DAL3(YIR032C)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Ureidoglycolate hydrolase, converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:ECL1(YGR146C)|FD-Score:4.27|P-value:9.58E-6||SGD DESC:Protein of unknown function; mitochondrial-dependent role in the extension of chronological lifespan; overexpression increases oxygen consumption and respiratory activity while deletion results in reduced oxygen consumption under conditions of caloric restriction; induced by iron homeostasis transcription factor Aft2p; multicopy suppressor of temperature sensitive hsf1 mutant; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:EDS1(YBR033W_p)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Putative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication Gene:EMP24(YGL200C)|FD-Score:3.1|P-value:9.78E-4||SGD DESC:Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles Gene:FET4(YMR319C)|FD-Score:4.63|P-value:1.87E-6||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FIG2(YCR089W)|FD-Score:4.9|P-value:4.75E-7||SGD DESC:Cell wall adhesin, expressed specifically during mating; may be involved in maintenance of cell wall integrity during mating Gene:FLC2(YAL053W)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication Gene:FLO11(YIR019C)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:GPI-anchored cell surface glycoprotein (flocculin); required for pseudohyphal formation, invasive growth, flocculation, and biofilms; transcriptionally regulated by the MAPK pathway (via Ste12p and Tec1p) and the cAMP pathway (via Flo8p); required for the formation of fibrous interconnections between cells in a colony of a wild S. cerevisiae strain Gene:FPR4(YLR449W)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase) (proline isomerase) localized to the nucleus; catalyzes isomerization of proline residues in histones H3 and H4, which affects lysine methylation of those histones Gene:FRE7(YOL152W)|FD-Score:3.17|P-value:7.51E-4||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low copper levels Gene:GLC8(YMR311C)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress Gene:GYP1(YOR070C)|FD-Score:3.26|P-value:5.61E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HAM1(YJR069C)|FD-Score:3.79|P-value:7.48E-5||SGD DESC:Nucleoside triphosphate pyrophosphohydrolase; active against a wide range of substrates including ITP, dITP and XTP; mediates exclusion of noncanonical purines and pyrimadines from deoxyribonucleoside triphosphate pools; mutant is sensitive to the base analog 6-N-hydroxylaminopurine; protein abundance increases in response to DNA replication stress Gene:HCR1(YLR192C)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:HEM14(YER014W)|FD-Score:4.37|P-value:6.27E-6||SGD DESC:Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides Gene:HIS2(YFR025C)|FD-Score:4|P-value:3.17E-5||SGD DESC:Histidinolphosphatase, catalyzes the eighth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control Gene:HOS2(YGL194C)|FD-Score:5.68|P-value:6.71E-9||SGD DESC:Histone deacetylase and subunit of Set3 and Rpd3L complexes; required for gene activation via specific deacetylation of lysines in H3 and H4 histone tails; subunit of the Set3 complex, a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; co-localizes with Cmr1p in nuclear foci in response to DNA damage by MMS Gene:IES2(YNL215W)|FD-Score:6.27|P-value:1.78E-10||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress Gene:IRS4(YKR019C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:IST3(YIR005W)|FD-Score:3.1|P-value:9.73E-4||SGD DESC:Component of the U2 snRNP, required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing Gene:LEA1(YPL213W)|FD-Score:-5.58|P-value:1.20E-8||SGD DESC:Component of U2 snRNP; disruption causes reduced U2 snRNP levels; physically interacts with Msl1p; putative homolog of human U2A' snRNP protein Gene:MBA1(YBR185C)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Membrane-associated mitochondrial ribosome receptor; forms a complex with Mdm38p that may facilitate recruitment of mRNA-specific translational activators to ribosomes; possible role in protein export from the matrix to inner membrane Gene:MMS22(YLR320W)|FD-Score:4.74|P-value:1.06E-6||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation Gene:MNN10(YDR245W)|FD-Score:5.04|P-value:2.39E-7||SGD DESC:Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family Gene:MRN1(YPL184C)|FD-Score:3.68|P-value:1.19E-4||SGD DESC:RNA-binding protein that may be involved in translational regulation; binds specific categories of mRNAs, including those that contain upstream open reading frames (uORFs) and internal ribosome entry sites (IRES); interacts genetically with chromatin remodelers and splicing factors, linking chromatin state, splicing and as a result mRNA maturation Gene:MRP7(YNL005C)|FD-Score:-3.25|P-value:5.84E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSS116(YDR194C)|FD-Score:-3.53|P-value:2.08E-4||SGD DESC:DEAD-box protein required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing Gene:NOT5(YPR072W)|FD-Score:-3.3|P-value:4.87E-4||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:NTC20(YBR188C)|FD-Score:-5.39|P-value:3.62E-8||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs Gene:OAF1(YAL051W)|FD-Score:3.88|P-value:5.19E-5||SGD DESC:Oleate-activated transcription factor; acts alone and as a heterodimer with Pip2p; activates genes involved in beta-oxidation of fatty acids and peroxisome organization and biogenesis; OAF1 has a paralog, PIP2, that arose from the whole genome duplication Gene:PEX34(YCL056C)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Peroxisomal integral membrane protein that regulates peroxisome populations; interacts with Pex11p, Pex25p, and Pex27p to control both constitutive peroxisome division and peroxisome morphology and abundance during peroxisome proliferation Gene:PHB2(YGR231C)|FD-Score:-3.72|P-value:9.92E-5||SGD DESC:Subunit of the prohibitin complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation Gene:PIN4(YBL051C)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:PMP1(YCR024C-A)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication Gene:PTH2(YBL057C)|FD-Score:3.4|P-value:3.31E-4||SGD DESC:One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth Gene:PYC1(YGL062W)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc2p; mutations in the human homolog are associated with lactic acidosis; PYC1 has a paralog, PYC2, that arose from the whole genome duplication Gene:RAD27(YKL113C)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family Gene:RGI1(YER067W)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Protein of unknown function; involved in energy metabolism under respiratory conditions; protein abundance is increased upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:RLF2(YPR018W)|FD-Score:-3.89|P-value:5.05E-5||SGD DESC:Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:RPL35B(YDL136W)|FD-Score:3.93|P-value:4.27E-5||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RPS0B(YLR048W)|FD-Score:4.77|P-value:9.09E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2 Gene:RPS12(YOR369C)|FD-Score:3.26|P-value:5.50E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S12, no bacterial homolog Gene:RPS16A(YMR143W)|FD-Score:3.25|P-value:5.81E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16A has a paralog, RPS16B, that arose from the whole genome duplication Gene:RSM24(YDR175C)|FD-Score:-3.48|P-value:2.51E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RTR2(YDR066C_p)|FD-Score:5.07|P-value:1.97E-7||SGD DESC:Protein of unknown function; exhibits genetic interactions with Rtr1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YDR066C is not an essential gene; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress; RTR2 has a paralog, RTR1, that arose from the whole genome duplication Gene:SCS7(YMR272C)|FD-Score:6.16|P-value:3.75E-10||SGD DESC:Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth Gene:SEE1(YIL064W)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Probable lysine methyltransferase involved in the dimethylation of eEF1A (Tef1p/Tef2p); sequence similarity to S-adenosylmethionine-dependent methyltransferases of the seven beta-strand family; role in vesicular transport Gene:SGM1(YJR134C)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:Protein of unknown function, required for wild-type growth rate on galactose and mannose; localizes to COPI coated vesicles and the Golgi apparatus Gene:SLT2(YHR030C)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNC1(YAL030W)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; proposed to be involved in endocytosis; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC1 has a paralog, SNC2, that arose from the whole genome duplication Gene:SOL4(YGR248W)|FD-Score:4.67|P-value:1.49E-6||SGD DESC:6-phosphogluconolactonase; protein abundance increases in response to DNA replication stress; SOL4 has a paralog, SOL3, that arose from the whole genome duplication Gene:SPO14(YKR031C)|FD-Score:3.17|P-value:7.52E-4||SGD DESC:Phospholipase D; catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions Gene:SPR6(YER115C)|FD-Score:8.55|P-value:6.34E-18||SGD DESC:Protein of unknown function, expressed during sporulation; not required for sporulation, but gene exhibits genetic interactions with other genes required for sporulation Gene:SRO77(YBL106C)|FD-Score:4.82|P-value:7.12E-7||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:SSK22(YCR073C)|FD-Score:6.24|P-value:2.18E-10||SGD DESC:MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; functionally redundant with, and homologous to, Ssk2p; interacts with and is activated by Ssk1p; phosphorylates Pbs2p Gene:TOS1(YBR162C)|FD-Score:4.32|P-value:7.95E-6||SGD DESC:Covalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C Gene:TRP3(YKL211C)|FD-Score:5.75|P-value:4.50E-9||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UBS1(YBR165W)|FD-Score:4.27|P-value:9.67E-6||SGD DESC:Ubiquitin-conjugating enzyme suppressor that functions as a general positive regulator of Cdc34p activity; nuclear protein that may represent a link between nucleocytoplasmic transport and ubiquitin ligase activity Gene:UNG1(YML021C)|FD-Score:4.68|P-value:1.43E-6||SGD DESC:Uracil-DNA glycosylase, required for repair of uracil in DNA formed by spontaneous cytosine deamination, not required for strand-specific mismatch repair, cell-cycle regulated, expressed in late G1, localizes to mitochondria and nucleus Gene:VPS17(YOR132W)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde protein transport; peripheral membrane protein that assembles onto the membrane with Vps5p to promote vesicle formation; required for recruiting the retromer complex to the endosome membranes Gene:VPS73(YGL104C)|FD-Score:-3.4|P-value:3.43E-4||SGD DESC:Mitochondrial protein; mutation affects vacuolar protein sorting; putative transporter; member of the sugar porter family Gene:YBR116C(YBR116C_d)|FD-Score:3.99|P-value:3.31E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene TKL2 Gene:YCR025C(YCR025C_d)|FD-Score:6.38|P-value:8.69E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR025C is not an essential gene Gene:YCR051W(YCR051W_p)|FD-Score:3.11|P-value:9.51E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene Gene:YDL011C(YDL011C_d)|FD-Score:3.94|P-value:4.07E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized ORF YDL010W Gene:YDR537C(YDR537C_d)|FD-Score:3.36|P-value:3.93E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, almost completely overlaps verified ORF PAD1/YDR538W Gene:YEL010W(YEL010W_d)|FD-Score:3.33|P-value:4.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER079W(YER079W_p)|FD-Score:-3.11|P-value:9.49E-4||SGD DESC:Putative protein of unknown function Gene:YER087C-A(YER087C-A_d)|FD-Score:3.25|P-value:5.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; overlaps the uncharacterized gene YER087W, a putative tRNA synthetase Gene:YFR057W(YFR057W_p)|FD-Score:-4.23|P-value:1.17E-5||SGD DESC:Putative protein of unknown function Gene:YGL199C(YGL199C_d)|FD-Score:3.89|P-value:5.11E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YIP4/YGL198W Gene:YGL262W(YGL262W_p)|FD-Score:3.98|P-value:3.38E-5||SGD DESC:Putative protein of unknown function; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein; YGL262W is not an essential gene Gene:YIL077C(YIL077C_p)|FD-Score:4.01|P-value:3.07E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YIL134C-A(YIL134C-A_p)|FD-Score:-4.18|P-value:1.47E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YJR020W(YJR020W_d)|FD-Score:-3.36|P-value:3.83E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR326W(YLR326W_p)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:Putative protein of unknown function, predicted to be palmitoylated Gene:YMR304C-A(YMR304C-A_d)|FD-Score:3.94|P-value:4.10E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SCW10 Gene:YNL146C-A(YNL146C-A_p)|FD-Score:3.14|P-value:8.44E-4||SGD DESC:Putative protein of unknown function Gene:YNL285W(YNL285W_d)|FD-Score:-3.61|P-value:1.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR072W(YOR072W_d)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YPK1(YKL126W)|FD-Score:5.53|P-value:1.62E-8||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL077C(YPL077C_p)|FD-Score:-4.41|P-value:5.16E-6||SGD DESC:Putative protein of unknown function; regulates PIS1 expression; mutant displays spore wall assembly defect in ether sensitivity screen; YPL077C is not an essential gene; YPL077C has a paralog, YBR197C, that arose from the whole genome duplication Gene:YPL109C(YPL109C_p)|FD-Score:4.56|P-value:2.60E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPL152W-A(YPL152W-A_p)|FD-Score:3.29|P-value:5.07E-4||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:ABM1(YJR108W)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Protein of unknown function, required for normal microtubule organization Gene:ACE2(YLR131C)|FD-Score:4.07|P-value:2.33E-5||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:AIM10(YER087W)|FD-Score:-7.43|P-value:5.24E-14||SGD DESC:Protein with similarity to tRNA synthetases; non-tagged protein is detected in purified mitochondria; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:ANP1(YEL036C)|FD-Score:-3.35|P-value:3.98E-4||SGD DESC:Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol Gene:APM4(YOL062C)|FD-Score:-5|P-value:2.79E-7||SGD DESC:Mu2-like subunit of the clathrin associated protein complex (AP-2); involved in vesicle transport Gene:ARC18(YLR370C)|FD-Score:3.13|P-value:8.83E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ATG9(YDL149W)|FD-Score:4.08|P-value:2.27E-5||SGD DESC:Transmembrane protein involved in forming Cvt and autophagic vesicles; cycles between the phagophore assembly site (PAS) and other cytosolic punctate structures, not found in autophagosomes; may be involved in membrane delivery to the PAS Gene:ATP19(YOL077W-A)|FD-Score:3.86|P-value:5.72E-5||SGD DESC:Subunit k of the mitochondrial F1F0 ATP synthase, which is a large enzyme complex required for ATP synthesis; associated only with the dimeric form of ATP synthase Gene:AVT5(YBL089W)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Putative transporter; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters; AVT5 has a paralog, AVT6, that arose from the whole genome duplication Gene:BRE4(YDL231C)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:Zinc finger protein containing five transmembrane domains; null mutant exhibits strongly fragmented vacuoles and sensitivity to brefeldin A, a drug which is known to affect intracellular transport Gene:BSP1(YPR171W)|FD-Score:4.49|P-value:3.52E-6||SGD DESC:Adapter that links synaptojanins Inp52p and Inp53p to the cortical actin cytoskeleton Gene:BUD14(YAR014C)|FD-Score:6.42|P-value:6.89E-11||SGD DESC:Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; inhibitor of the actin assembly factor Bnr1p (formin); diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress Gene:BUD21(YOR078W)|FD-Score:-3.48|P-value:2.54E-4||SGD DESC:Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated as bud-site selection mutant that displays a random budding pattern Gene:CEX1(YOR112W)|FD-Score:-3.24|P-value:5.99E-4||SGD DESC:Cytoplasmic component of the nuclear aminoacylation-dependent tRNA export pathway; interacts with nuclear pore component Nup116p; copurifies with tRNA export receptors Los1p and Msn5p, as well as eIF-1a and the RAN GTPase Gsp1p Gene:CHS3(YBR023C)|FD-Score:4.96|P-value:3.48E-7||SGD DESC:Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan Gene:CLC1(YGR167W)|FD-Score:3.12|P-value:9.16E-4||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:COX12(YLR038C)|FD-Score:9.06|P-value:6.60E-20||SGD DESC:Subunit VIb of cytochrome c oxidase; cytochrome c oxidase is also known as respiratory Complex IV and is the terminal member of the mitochondrial inner membrane electron transport chain; required for assembly of cytochrome c oxidase but not required for activity after assembly; phosphorylated; easily released from the intermembrane space, suggesting a loose association with Complex IV Gene:CPR6(YLR216C)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity; protein abundance increases in response to DNA replication stress Gene:CSF1(YLR087C)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CSM3(YMR048W)|FD-Score:5.38|P-value:3.78E-8||SGD DESC:Replication fork associated factor; required for stable replication fork pausing; component of the DNA replication checkpoint pathway; required for accurate chromosome segregation during meiosis; forms nuclear foci upon DNA replication stress Gene:CSR1(YLR380W)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Phosphatidylinositol transfer protein; has a potential role in regulating lipid and fatty acid metabolism under heme-depleted conditions; interacts specifically with thioredoxin peroxidase; may have a role in oxidative stress resistance; protein abundance increases in response to DNA replication stress Gene:CTR1(YPR124W)|FD-Score:-3.96|P-value:3.71E-5||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CUE1(YMR264W)|FD-Score:9.05|P-value:7.47E-20||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:CUP9(YPL177C)|FD-Score:4.81|P-value:7.46E-7||SGD DESC:Homeodomain-containing transcriptional repressor; regulates expression of PTR2, which encodes a major peptide transporter; imported peptides activate ubiquitin-dependent proteolysis, resulting in degradation of Cup9p and de-repression of PTR2 transcription; CUP9 has a paralog, TOS8, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:CWH43(YCR017C)|FD-Score:-3.55|P-value:1.94E-4||SGD DESC:Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion Gene:DAL3(YIR032C)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Ureidoglycolate hydrolase, converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:ECL1(YGR146C)|FD-Score:4.27|P-value:9.58E-6||SGD DESC:Protein of unknown function; mitochondrial-dependent role in the extension of chronological lifespan; overexpression increases oxygen consumption and respiratory activity while deletion results in reduced oxygen consumption under conditions of caloric restriction; induced by iron homeostasis transcription factor Aft2p; multicopy suppressor of temperature sensitive hsf1 mutant; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:EDS1(YBR033W_p)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Putative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication Gene:EMP24(YGL200C)|FD-Score:3.1|P-value:9.78E-4||SGD DESC:Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles Gene:FET4(YMR319C)|FD-Score:4.63|P-value:1.87E-6||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FIG2(YCR089W)|FD-Score:4.9|P-value:4.75E-7||SGD DESC:Cell wall adhesin, expressed specifically during mating; may be involved in maintenance of cell wall integrity during mating Gene:FLC2(YAL053W)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication Gene:FLO11(YIR019C)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:GPI-anchored cell surface glycoprotein (flocculin); required for pseudohyphal formation, invasive growth, flocculation, and biofilms; transcriptionally regulated by the MAPK pathway (via Ste12p and Tec1p) and the cAMP pathway (via Flo8p); required for the formation of fibrous interconnections between cells in a colony of a wild S. cerevisiae strain Gene:FPR4(YLR449W)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase) (proline isomerase) localized to the nucleus; catalyzes isomerization of proline residues in histones H3 and H4, which affects lysine methylation of those histones Gene:FRE7(YOL152W)|FD-Score:3.17|P-value:7.51E-4||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low copper levels Gene:GLC8(YMR311C)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress Gene:GYP1(YOR070C)|FD-Score:3.26|P-value:5.61E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HAM1(YJR069C)|FD-Score:3.79|P-value:7.48E-5||SGD DESC:Nucleoside triphosphate pyrophosphohydrolase; active against a wide range of substrates including ITP, dITP and XTP; mediates exclusion of noncanonical purines and pyrimadines from deoxyribonucleoside triphosphate pools; mutant is sensitive to the base analog 6-N-hydroxylaminopurine; protein abundance increases in response to DNA replication stress Gene:HCR1(YLR192C)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:HEM14(YER014W)|FD-Score:4.37|P-value:6.27E-6||SGD DESC:Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides Gene:HIS2(YFR025C)|FD-Score:4|P-value:3.17E-5||SGD DESC:Histidinolphosphatase, catalyzes the eighth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control Gene:HOS2(YGL194C)|FD-Score:5.68|P-value:6.71E-9||SGD DESC:Histone deacetylase and subunit of Set3 and Rpd3L complexes; required for gene activation via specific deacetylation of lysines in H3 and H4 histone tails; subunit of the Set3 complex, a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; co-localizes with Cmr1p in nuclear foci in response to DNA damage by MMS Gene:IES2(YNL215W)|FD-Score:6.27|P-value:1.78E-10||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress Gene:IRS4(YKR019C)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:IST3(YIR005W)|FD-Score:3.1|P-value:9.73E-4||SGD DESC:Component of the U2 snRNP, required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing Gene:LEA1(YPL213W)|FD-Score:-5.58|P-value:1.20E-8||SGD DESC:Component of U2 snRNP; disruption causes reduced U2 snRNP levels; physically interacts with Msl1p; putative homolog of human U2A' snRNP protein Gene:MBA1(YBR185C)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Membrane-associated mitochondrial ribosome receptor; forms a complex with Mdm38p that may facilitate recruitment of mRNA-specific translational activators to ribosomes; possible role in protein export from the matrix to inner membrane Gene:MMS22(YLR320W)|FD-Score:4.74|P-value:1.06E-6||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation Gene:MNN10(YDR245W)|FD-Score:5.04|P-value:2.39E-7||SGD DESC:Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family Gene:MRN1(YPL184C)|FD-Score:3.68|P-value:1.19E-4||SGD DESC:RNA-binding protein that may be involved in translational regulation; binds specific categories of mRNAs, including those that contain upstream open reading frames (uORFs) and internal ribosome entry sites (IRES); interacts genetically with chromatin remodelers and splicing factors, linking chromatin state, splicing and as a result mRNA maturation Gene:MRP7(YNL005C)|FD-Score:-3.25|P-value:5.84E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSS116(YDR194C)|FD-Score:-3.53|P-value:2.08E-4||SGD DESC:DEAD-box protein required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing Gene:NOT5(YPR072W)|FD-Score:-3.3|P-value:4.87E-4||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:NTC20(YBR188C)|FD-Score:-5.39|P-value:3.62E-8||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs Gene:OAF1(YAL051W)|FD-Score:3.88|P-value:5.19E-5||SGD DESC:Oleate-activated transcription factor; acts alone and as a heterodimer with Pip2p; activates genes involved in beta-oxidation of fatty acids and peroxisome organization and biogenesis; OAF1 has a paralog, PIP2, that arose from the whole genome duplication Gene:PEX34(YCL056C)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Peroxisomal integral membrane protein that regulates peroxisome populations; interacts with Pex11p, Pex25p, and Pex27p to control both constitutive peroxisome division and peroxisome morphology and abundance during peroxisome proliferation Gene:PHB2(YGR231C)|FD-Score:-3.72|P-value:9.92E-5||SGD DESC:Subunit of the prohibitin complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation Gene:PIN4(YBL051C)|FD-Score:3.31|P-value:4.73E-4||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:PMP1(YCR024C-A)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication Gene:PTH2(YBL057C)|FD-Score:3.4|P-value:3.31E-4||SGD DESC:One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth Gene:PYC1(YGL062W)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc2p; mutations in the human homolog are associated with lactic acidosis; PYC1 has a paralog, PYC2, that arose from the whole genome duplication Gene:RAD27(YKL113C)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family Gene:RGI1(YER067W)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Protein of unknown function; involved in energy metabolism under respiratory conditions; protein abundance is increased upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:RLF2(YPR018W)|FD-Score:-3.89|P-value:5.05E-5||SGD DESC:Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:RPL35B(YDL136W)|FD-Score:3.93|P-value:4.27E-5||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RPS0B(YLR048W)|FD-Score:4.77|P-value:9.09E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2 Gene:RPS12(YOR369C)|FD-Score:3.26|P-value:5.50E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S12, no bacterial homolog Gene:RPS16A(YMR143W)|FD-Score:3.25|P-value:5.81E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16A has a paralog, RPS16B, that arose from the whole genome duplication Gene:RSM24(YDR175C)|FD-Score:-3.48|P-value:2.51E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RTR2(YDR066C_p)|FD-Score:5.07|P-value:1.97E-7||SGD DESC:Protein of unknown function; exhibits genetic interactions with Rtr1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YDR066C is not an essential gene; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress; RTR2 has a paralog, RTR1, that arose from the whole genome duplication Gene:SCS7(YMR272C)|FD-Score:6.16|P-value:3.75E-10||SGD DESC:Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth Gene:SEE1(YIL064W)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Probable lysine methyltransferase involved in the dimethylation of eEF1A (Tef1p/Tef2p); sequence similarity to S-adenosylmethionine-dependent methyltransferases of the seven beta-strand family; role in vesicular transport Gene:SGM1(YJR134C)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:Protein of unknown function, required for wild-type growth rate on galactose and mannose; localizes to COPI coated vesicles and the Golgi apparatus Gene:SLT2(YHR030C)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNC1(YAL030W)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; proposed to be involved in endocytosis; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC1 has a paralog, SNC2, that arose from the whole genome duplication Gene:SOL4(YGR248W)|FD-Score:4.67|P-value:1.49E-6||SGD DESC:6-phosphogluconolactonase; protein abundance increases in response to DNA replication stress; SOL4 has a paralog, SOL3, that arose from the whole genome duplication Gene:SPO14(YKR031C)|FD-Score:3.17|P-value:7.52E-4||SGD DESC:Phospholipase D; catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions Gene:SPR6(YER115C)|FD-Score:8.55|P-value:6.34E-18||SGD DESC:Protein of unknown function, expressed during sporulation; not required for sporulation, but gene exhibits genetic interactions with other genes required for sporulation Gene:SRO77(YBL106C)|FD-Score:4.82|P-value:7.12E-7||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:SSK22(YCR073C)|FD-Score:6.24|P-value:2.18E-10||SGD DESC:MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; functionally redundant with, and homologous to, Ssk2p; interacts with and is activated by Ssk1p; phosphorylates Pbs2p Gene:TOS1(YBR162C)|FD-Score:4.32|P-value:7.95E-6||SGD DESC:Covalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C Gene:TRP3(YKL211C)|FD-Score:5.75|P-value:4.50E-9||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UBS1(YBR165W)|FD-Score:4.27|P-value:9.67E-6||SGD DESC:Ubiquitin-conjugating enzyme suppressor that functions as a general positive regulator of Cdc34p activity; nuclear protein that may represent a link between nucleocytoplasmic transport and ubiquitin ligase activity Gene:UNG1(YML021C)|FD-Score:4.68|P-value:1.43E-6||SGD DESC:Uracil-DNA glycosylase, required for repair of uracil in DNA formed by spontaneous cytosine deamination, not required for strand-specific mismatch repair, cell-cycle regulated, expressed in late G1, localizes to mitochondria and nucleus Gene:VPS17(YOR132W)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde protein transport; peripheral membrane protein that assembles onto the membrane with Vps5p to promote vesicle formation; required for recruiting the retromer complex to the endosome membranes Gene:VPS73(YGL104C)|FD-Score:-3.4|P-value:3.43E-4||SGD DESC:Mitochondrial protein; mutation affects vacuolar protein sorting; putative transporter; member of the sugar porter family Gene:YBR116C(YBR116C_d)|FD-Score:3.99|P-value:3.31E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene TKL2 Gene:YCR025C(YCR025C_d)|FD-Score:6.38|P-value:8.69E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR025C is not an essential gene Gene:YCR051W(YCR051W_p)|FD-Score:3.11|P-value:9.51E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene Gene:YDL011C(YDL011C_d)|FD-Score:3.94|P-value:4.07E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the uncharacterized ORF YDL010W Gene:YDR537C(YDR537C_d)|FD-Score:3.36|P-value:3.93E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, almost completely overlaps verified ORF PAD1/YDR538W Gene:YEL010W(YEL010W_d)|FD-Score:3.33|P-value:4.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER079W(YER079W_p)|FD-Score:-3.11|P-value:9.49E-4||SGD DESC:Putative protein of unknown function Gene:YER087C-A(YER087C-A_d)|FD-Score:3.25|P-value:5.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; overlaps the uncharacterized gene YER087W, a putative tRNA synthetase Gene:YFR057W(YFR057W_p)|FD-Score:-4.23|P-value:1.17E-5||SGD DESC:Putative protein of unknown function Gene:YGL199C(YGL199C_d)|FD-Score:3.89|P-value:5.11E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YIP4/YGL198W Gene:YGL262W(YGL262W_p)|FD-Score:3.98|P-value:3.38E-5||SGD DESC:Putative protein of unknown function; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein; YGL262W is not an essential gene Gene:YIL077C(YIL077C_p)|FD-Score:4.01|P-value:3.07E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YIL134C-A(YIL134C-A_p)|FD-Score:-4.18|P-value:1.47E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YJR020W(YJR020W_d)|FD-Score:-3.36|P-value:3.83E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR326W(YLR326W_p)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:Putative protein of unknown function, predicted to be palmitoylated Gene:YMR304C-A(YMR304C-A_d)|FD-Score:3.94|P-value:4.10E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SCW10 Gene:YNL146C-A(YNL146C-A_p)|FD-Score:3.14|P-value:8.44E-4||SGD DESC:Putative protein of unknown function Gene:YNL285W(YNL285W_d)|FD-Score:-3.61|P-value:1.52E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR072W(YOR072W_d)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YPK1(YKL126W)|FD-Score:5.53|P-value:1.62E-8||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL077C(YPL077C_p)|FD-Score:-4.41|P-value:5.16E-6||SGD DESC:Putative protein of unknown function; regulates PIS1 expression; mutant displays spore wall assembly defect in ether sensitivity screen; YPL077C is not an essential gene; YPL077C has a paralog, YBR197C, that arose from the whole genome duplication Gene:YPL109C(YPL109C_p)|FD-Score:4.56|P-value:2.60E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPL152W-A(YPL152W-A_p)|FD-Score:3.29|P-value:5.07E-4||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR069C8.441.56E-172.15RRP4Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2)
YKL165C8.295.79E-172.15MCD4Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes
YDR339C6.144.08E-100.22FCF1Putative PINc domain nuclease required for early cleavages of 35S pre-rRNA and maturation of 18S rRNA; component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; copurifies with Faf1p
YBR190W_d5.921.60E-90.01YBR190W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W
YLR243W_p5.911.70E-90.11GPN3_pPutative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn2p
YML043C5.803.28E-90.51RRN11Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
YML092C5.296.04E-80.08PRE8Alpha 2 subunit of the 20S proteasome
YPL075W5.229.15E-80.24GCR1Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p
YBR253W4.973.30E-70.05SRB6Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
YCL004W4.934.20E-70.23PGS1Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis
YKR083C4.701.32E-60.29DAD2Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
YOR181W4.405.34E-60.13LAS17Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP)
YPL175W4.279.80E-60.32SPT14UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins
YKL060C3.953.98E-50.08FBA1Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversion of fructose 1,6 bisphosphate to glyceraldehyde-3-P and dihydroxyacetone-P; locates to mitochondrial outer surface upon oxidative stress
YPL237W3.865.59E-50.21SUI3Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR038C9.066.60E-20COX12Subunit VIb of cytochrome c oxidase; cytochrome c oxidase is also known as respiratory Complex IV and is the terminal member of the mitochondrial inner membrane electron transport chain; required for assembly of cytochrome c oxidase but not required for activity after assembly; phosphorylated; easily released from the intermembrane space, suggesting a loose association with Complex IV
YMR264W9.057.47E-20CUE1Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication
YER115C8.556.34E-18SPR6Protein of unknown function, expressed during sporulation; not required for sporulation, but gene exhibits genetic interactions with other genes required for sporulation
YAR014C6.426.89E-11BUD14Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; inhibitor of the actin assembly factor Bnr1p (formin); diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress
YCR025C_d6.388.69E-11YCR025C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR025C is not an essential gene
YNL215W6.271.78E-10IES2Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress
YCR073C6.242.18E-10SSK22MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; functionally redundant with, and homologous to, Ssk2p; interacts with and is activated by Ssk1p; phosphorylates Pbs2p
YMR272C6.163.75E-10SCS7Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth
YKL211C5.754.50E-9TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YGL194C5.686.71E-9HOS2Histone deacetylase and subunit of Set3 and Rpd3L complexes; required for gene activation via specific deacetylation of lysines in H3 and H4 histone tails; subunit of the Set3 complex, a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; co-localizes with Cmr1p in nuclear foci in response to DNA damage by MMS
YKL126W5.531.62E-8YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YMR048W5.383.78E-8CSM3Replication fork associated factor; required for stable replication fork pausing; component of the DNA replication checkpoint pathway; required for accurate chromosome segregation during meiosis; forms nuclear foci upon DNA replication stress
YDR066C_p5.071.97E-7RTR2_pProtein of unknown function; exhibits genetic interactions with Rtr1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YDR066C is not an essential gene; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress; RTR2 has a paralog, RTR1, that arose from the whole genome duplication
YDR245W5.042.39E-7MNN10Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family
YBR023C4.963.48E-7CHS3Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan

GO enrichment analysis for SGTC_2780
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0921.57E-12SGTC_12212958-7315 257.0 μMChemDiv (Drug-like library)45627050.15625
0.0863.26E-11SGTC_14113909-8223 150.0 μMChemDiv (Drug-like library)X14110.0952381
0.0863.92E-11SGTC_12161165-0504 9.7 μMChemDiv (Drug-like library)63040040.0952381
0.0772.84E-9SGTC_2231micatex 6.9 μMMiscellaneous207370.0545455
0.0766.22E-9SGTC_21945927483 104.5 μMChembridge (Fragment library)7609790.114754
0.0731.97E-8SGTC_5810958-0271 211.0 μMChemDiv (Drug-like library)45911450.103896
0.0732.42E-8SGTC_813k007-0158 173.0 μMChemDiv (Drug-like library)65239160.0882353fatty acid desaturase (OLE1)
0.0722.97E-8SGTC_2409alverine citrate 64.0 μMMiscellaneous217180.107143
0.0723.16E-8SGTC_6371181-0519 7.3 μMChemDiv (Drug-like library)62520910.0724638fatty acid desaturase (OLE1)
0.0714.54E-8SGTC_23127706453 155.7 μMChembridge (Fragment library)9760980.125
0.0715.27E-8SGTC_1064honokiol 6.8 μMNIH Clinical Collection723030.0606061
0.0691.12E-7SGTC_1833st055984 71.0 μMTimTec (Natural product derivative library)6762880.266667
0.0663.75E-7SGTC_7764476-4378 112.0 μMChemDiv (Drug-like library)28920080.131579ERG2
0.0656.62E-7SGTC_11941488-0194 225.0 μMChemDiv (Drug-like library)13914520.0547945
0.0649.77E-7SGTC_1838st055369 84.5 μMTimTec (Natural product derivative library)6760360.166667

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1970st07012235.8 μM0.423077102928TimTec (Natural product derivative library)254.280563.08303TSC3-RPN4
SGTC_10184259-001328.1 μM0.3225816801262ChemDiv (Drug-like library)318.75491.44935
SGTC_14204'-methoxyflavone42 μM0.30909177793ChemDiv (Drug-like library)252.264683.1203
SGTC_26344'-methoxyflavone100 μM0.30909177793Microsource (Natural product library)252.264683.1203
SGTC_1864st05764530.4 μM0.303571688666TimTec (Natural product derivative library)282.290662.80804amide catabolism
SGTC_11423253-14089.78 μM0.301887256655ChemDiv (Drug-like library)276.332443.94123
SGTC_26222-methoxyxanthone59.94 μM0.30188771034Microsource (Natural product library)226.22742.94803
SGTC_1745st08149574 μM0.313842402TimTec (Natural product derivative library)270.279962.94814RNA processing & uracil transport
SGTC_15494'-methoxyflavonol74.6 μM0.29824697141TimTec (Pure natural product library)268.264082.58214
SGTC_328k915-012063.5 μM0.2923086624543ChemDiv (Drug-like library)388.458964.48825TSC3-RPN4
SGTC_20214019055173 μM0.28846244827708Chembridge (Fragment library)163.216321.26112endomembrane recycling