5284540

N-(3-chlorophenyl)-3,4-dimethoxybenzamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2787
Screen concentration 71.4 μM
Source Chembridge (Drug-like library)
PubChem CID 736958
SMILES COC1=C(C=C(C=C1)C(=O)NC2=CC(=CC=C2)Cl)OC
Standardized SMILES COc1ccc(cc1OC)C(=O)Nc2cccc(Cl)c2
Molecular weight 291.7296
ALogP 3.25
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.62
% growth inhibition (Hom. pool) 3.43


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 736958
Download HIP data (tab-delimited text)  (excel)
Gene:ADE13(YLR359W)|FD-Score:-3.57|P-value:1.80E-4|Clearance:0||SGD DESC:Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency Gene:ARH1(YDR376W)|FD-Score:3.15|P-value:8.17E-4|Clearance:0||SGD DESC:Oxidoreductase of the mitochondrial inner membrane, involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viability Gene:BRX1(YOL077C)|FD-Score:5.19|P-value:1.04E-7|Clearance:0.03||SGD DESC:Nucleolar protein, constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif Gene:CDC4(YFL009W)|FD-Score:3.11|P-value:9.22E-4|Clearance:0||SGD DESC:F-box protein required for G1/S and G2/M transition, associates with Skp1p and Cdc53p to form a complex, SCFCdc4, which acts as ubiquitin-protein ligase directing ubiquitination of the phosphorylated CDK inhibitor Sic1p Gene:COG2(YGR120C)|FD-Score:-3.19|P-value:7.21E-4|Clearance:0||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:HSH49(YOR319W)|FD-Score:4.01|P-value:3.00E-5|Clearance:0.01||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:INO80(YGL150C)|FD-Score:3.15|P-value:8.28E-4|Clearance:0.03||SGD DESC:ATPase and nucleosome spacing factor, subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; has a role in modulating stress gene transcription Gene:JIP5(YPR169W)|FD-Score:3.62|P-value:1.50E-4|Clearance:0.19||SGD DESC:Essential protein required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein Gene:LSM8(YJR022W)|FD-Score:4.91|P-value:4.48E-7|Clearance:0.9||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) that is part of spliceosomal U6 snRNP and is also implicated in processing of pre-tRNA, pre-snoRNA, and pre-rRNA Gene:RIX1(YHR197W)|FD-Score:-3.81|P-value:6.98E-5|Clearance:0||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene Gene:RKI1(YOR095C)|FD-Score:5.16|P-value:1.21E-7|Clearance:0.1||SGD DESC:Ribose-5-phosphate ketol-isomerase, catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis Gene:RPP1(YHR062C)|FD-Score:3.34|P-value:4.12E-4|Clearance:0.05||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS31(YLR167W)|FD-Score:-3.37|P-value:3.71E-4|Clearance:0||SGD DESC:Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog Gene:RRP7(YCL031C)|FD-Score:-3.51|P-value:2.24E-4|Clearance:0||SGD DESC:Essential protein involved in rRNA processing and ribosome biogenesis; protein abundance increases in response to DNA replication stress Gene:SAR1(YPL218W)|FD-Score:4.01|P-value:3.09E-5|Clearance:0.08||SGD DESC:GTPase, GTP-binding protein of the ARF family, component of COPII coat of vesicles; required for transport vesicle formation during ER to Golgi protein transport Gene:SEC31(YDL195W)|FD-Score:3.64|P-value:1.38E-4|Clearance:0.02||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SOF1(YLL011W)|FD-Score:3.69|P-value:1.13E-4|Clearance:0.05||SGD DESC:Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p Gene:SRB4(YER022W)|FD-Score:9.44|P-value:1.79E-21|Clearance:4.25||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination Gene:STH1(YIL126W)|FD-Score:-3.13|P-value:8.78E-4|Clearance:0||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:TEN1(YLR010C)|FD-Score:5.02|P-value:2.58E-7|Clearance:0.11||SGD DESC:Protein that regulates telomeric length; protects telomeric ends in a complex with Cdc13p and Stn1p Gene:TIM23(YNR017W)|FD-Score:3.11|P-value:9.25E-4|Clearance:0.07||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein import into mitochondrial matrix and inner membrane; with Tim17p, contributes to architecture and function of the import channel Gene:TRR1(YDR353W)|FD-Score:-5.27|P-value:6.75E-8|Clearance:0||SGD DESC:Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication Gene:UTP21(YLR409C)|FD-Score:3.18|P-value:7.38E-4|Clearance:0.03||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; synthetic defect with STI1 Hsp90 cochaperone; human homolog linked to glaucoma Gene:YAE1(YJR067C)|FD-Score:3.42|P-value:3.09E-4|Clearance:0.08||SGD DESC:Protein that forms a complex with Lto1p and Rli1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species Gene:YDR187C(YDR187C_d)|FD-Score:5.06|P-value:2.08E-7|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YKT6(YKL196C)|FD-Score:3.92|P-value:4.40E-5|Clearance:0.23||SGD DESC:Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus Gene:YLR339C(YLR339C_d)|FD-Score:-3.49|P-value:2.41E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPP0 Gene:YPR136C(YPR136C_d)|FD-Score:3.29|P-value:5.00E-4|Clearance:0.11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9 Gene:ADE13(YLR359W)|FD-Score:-3.57|P-value:1.80E-4|Clearance:0||SGD DESC:Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency Gene:ARH1(YDR376W)|FD-Score:3.15|P-value:8.17E-4|Clearance:0||SGD DESC:Oxidoreductase of the mitochondrial inner membrane, involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viability Gene:BRX1(YOL077C)|FD-Score:5.19|P-value:1.04E-7|Clearance:0.03||SGD DESC:Nucleolar protein, constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif Gene:CDC4(YFL009W)|FD-Score:3.11|P-value:9.22E-4|Clearance:0||SGD DESC:F-box protein required for G1/S and G2/M transition, associates with Skp1p and Cdc53p to form a complex, SCFCdc4, which acts as ubiquitin-protein ligase directing ubiquitination of the phosphorylated CDK inhibitor Sic1p Gene:COG2(YGR120C)|FD-Score:-3.19|P-value:7.21E-4|Clearance:0||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:HSH49(YOR319W)|FD-Score:4.01|P-value:3.00E-5|Clearance:0.01||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:INO80(YGL150C)|FD-Score:3.15|P-value:8.28E-4|Clearance:0.03||SGD DESC:ATPase and nucleosome spacing factor, subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; has a role in modulating stress gene transcription Gene:JIP5(YPR169W)|FD-Score:3.62|P-value:1.50E-4|Clearance:0.19||SGD DESC:Essential protein required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein Gene:LSM8(YJR022W)|FD-Score:4.91|P-value:4.48E-7|Clearance:0.9||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) that is part of spliceosomal U6 snRNP and is also implicated in processing of pre-tRNA, pre-snoRNA, and pre-rRNA Gene:RIX1(YHR197W)|FD-Score:-3.81|P-value:6.98E-5|Clearance:0||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene Gene:RKI1(YOR095C)|FD-Score:5.16|P-value:1.21E-7|Clearance:0.1||SGD DESC:Ribose-5-phosphate ketol-isomerase, catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis Gene:RPP1(YHR062C)|FD-Score:3.34|P-value:4.12E-4|Clearance:0.05||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS31(YLR167W)|FD-Score:-3.37|P-value:3.71E-4|Clearance:0||SGD DESC:Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog Gene:RRP7(YCL031C)|FD-Score:-3.51|P-value:2.24E-4|Clearance:0||SGD DESC:Essential protein involved in rRNA processing and ribosome biogenesis; protein abundance increases in response to DNA replication stress Gene:SAR1(YPL218W)|FD-Score:4.01|P-value:3.09E-5|Clearance:0.08||SGD DESC:GTPase, GTP-binding protein of the ARF family, component of COPII coat of vesicles; required for transport vesicle formation during ER to Golgi protein transport Gene:SEC31(YDL195W)|FD-Score:3.64|P-value:1.38E-4|Clearance:0.02||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SOF1(YLL011W)|FD-Score:3.69|P-value:1.13E-4|Clearance:0.05||SGD DESC:Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p Gene:SRB4(YER022W)|FD-Score:9.44|P-value:1.79E-21|Clearance:4.25||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination Gene:STH1(YIL126W)|FD-Score:-3.13|P-value:8.78E-4|Clearance:0||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:TEN1(YLR010C)|FD-Score:5.02|P-value:2.58E-7|Clearance:0.11||SGD DESC:Protein that regulates telomeric length; protects telomeric ends in a complex with Cdc13p and Stn1p Gene:TIM23(YNR017W)|FD-Score:3.11|P-value:9.25E-4|Clearance:0.07||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein import into mitochondrial matrix and inner membrane; with Tim17p, contributes to architecture and function of the import channel Gene:TRR1(YDR353W)|FD-Score:-5.27|P-value:6.75E-8|Clearance:0||SGD DESC:Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication Gene:UTP21(YLR409C)|FD-Score:3.18|P-value:7.38E-4|Clearance:0.03||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; synthetic defect with STI1 Hsp90 cochaperone; human homolog linked to glaucoma Gene:YAE1(YJR067C)|FD-Score:3.42|P-value:3.09E-4|Clearance:0.08||SGD DESC:Protein that forms a complex with Lto1p and Rli1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species Gene:YDR187C(YDR187C_d)|FD-Score:5.06|P-value:2.08E-7|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YKT6(YKL196C)|FD-Score:3.92|P-value:4.40E-5|Clearance:0.23||SGD DESC:Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus Gene:YLR339C(YLR339C_d)|FD-Score:-3.49|P-value:2.41E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPP0 Gene:YPR136C(YPR136C_d)|FD-Score:3.29|P-value:5.00E-4|Clearance:0.11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 736958
Download HOP data (tab-delimited text)  (excel)
Gene:ABZ1(YNR033W)|FD-Score:3.11|P-value:9.43E-4||SGD DESC:Para-aminobenzoate (PABA) synthase; has similarity to Escherichia coli PABA synthase components PabA and PabB; required for the synthesis of para-aminobenzoic acid, an important intermediate for folate and ubiquinone Q biosynthesis; protein abundance increases in response to DNA replication stress Gene:ACE2(YLR131C)|FD-Score:4.55|P-value:2.65E-6||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:AFT2(YPL202C)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; AFT2 has a paralog, AFT1, that arose from the whole genome duplication Gene:ALP1(YNL270C)|FD-Score:6.13|P-value:4.29E-10||SGD DESC:Arginine transporter; expression is normally very low and it is unclear what conditions would induce significant expression; ALP1 has a paralog, CAN1, that arose from the whole genome duplication Gene:AMA1(YGR225W)|FD-Score:4.06|P-value:2.40E-5||SGD DESC:Activator of meiotic anaphase promoting complex (APC/C); Cdc20p family member; required for initiation of spore wall assembly; required for Clb1p degradation during meiosis Gene:APE2(YKL157W)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Aminopeptidase yscII; may have a role in obtaining leucine from dipeptide substrates; APE2 has a paralog, AAP1, that arose from the whole genome duplication Gene:ARP1(YHR129C)|FD-Score:4.46|P-value:4.03E-6||SGD DESC:Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin Gene:AST1(YBL069W)|FD-Score:3.14|P-value:8.46E-4||SGD DESC:Lipid raft associated protein; interacts with the plasma membrane ATPase Pma1p and has a role in its targeting to the plasma membrane by influencing its incorporation into lipid rafts; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST1 has a paralog, AST2, that arose from the whole genome duplication Gene:ATS1(YAL020C)|FD-Score:4.57|P-value:2.42E-6||SGD DESC:Protein required, with Elongator complex, Kti11p, and Kti12p, for modification of wobble nucleosides in tRNA; has a potential role in regulatory interactions between microtubules and the cell cycle Gene:BRE5(YNR051C)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A Gene:CPA1(YOR303W)|FD-Score:-3.45|P-value:2.77E-4||SGD DESC:Small subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor; translationally regulated by an attenuator peptide encoded by YOR302W within the CPA1 mRNA 5'-leader Gene:DDI1(YER143W)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:DNA damage-inducible v-SNARE binding protein with a role in suppression of protein secretion; may play a role in S-phase checkpoint control; has ubiquitin-associated (UBA), ubiquitin-like (UBL), and retroviral-like proteinase (RVP) domains Gene:DEP1(YAL013W)|FD-Score:3.34|P-value:4.25E-4||SGD DESC:Transcriptional modulator involved in regulation of structural phospholipid biosynthesis genes and metabolically unrelated genes, as well as maintenance of telomeres, mating efficiency, and sporulation Gene:DFG16(YOR030W)|FD-Score:3.11|P-value:9.25E-4||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:ENV10(YLR065C)|FD-Score:3.33|P-value:4.33E-4||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:FEN2(YCR028C)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:FET4(YMR319C)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FMP21(YBR269C_p)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GDH1(YOR375C)|FD-Score:4.56|P-value:2.62E-6||SGD DESC:NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh3p; expression regulated by nitrogen and carbon sources; GDH1 has a paralog, GDH3, that arose from the whole genome duplication Gene:GUT1(YHL032C)|FD-Score:14|P-value:1.44E-44||SGD DESC:Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p Gene:GYP1(YOR070C)|FD-Score:3.16|P-value:7.89E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HDA2(YDR295C)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex containing an Hda1p homodimer and an Hda2p-Hda3p heterodimer; involved in telomere maintenance Gene:ICT1(YLR099C)|FD-Score:6.34|P-value:1.15E-10||SGD DESC:Lysophosphatidic acid acyltransferase; responsible for enhanced phospholipid synthesis during organic solvent stress; null displays increased sensitivity to Calcofluor white; highly expressed during organic solvent stress; ICT1 has a paralog, ECM18, that arose from the whole genome duplication Gene:IRC25(YLR021W)|FD-Score:3.1|P-value:9.82E-4||SGD DESC:Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions Gene:IRS4(YKR019C)|FD-Score:3.19|P-value:7.03E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LAC1(YKL008C)|FD-Score:-5.41|P-value:3.10E-8||SGD DESC:Ceramide synthase component, involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lag1p Gene:LEA1(YPL213W)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Component of U2 snRNP; disruption causes reduced U2 snRNP levels; physically interacts with Msl1p; putative homolog of human U2A' snRNP protein Gene:LRP1(YHR081W)|FD-Score:-4.07|P-value:2.33E-5||SGD DESC:Nuclear exosome-associated nucleic acid binding protein; involved in RNA processing, surveillance, degradation, tethering, and export; homolog of mammalian nuclear matrix protein C1D involved in regulation of DNA repair and recombination Gene:MMS22(YLR320W)|FD-Score:-3.2|P-value:6.95E-4||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation Gene:MMT2(YPL224C)|FD-Score:4.48|P-value:3.74E-6||SGD DESC:Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt1p Gene:MNN10(YDR245W)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family Gene:MSH6(YDR097C)|FD-Score:-3.79|P-value:7.55E-5||SGD DESC:Protein required for mismatch repair in mitosis and meiosis, forms a complex with Msh2p to repair both single-base & insertion-deletion mispairs; also involved in interstrand cross-link repair; potentially phosphorylated by Cdc28p Gene:OMS1(YDR316W)|FD-Score:3.82|P-value:6.75E-5||SGD DESC:Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations Gene:PFS1(YHR185C)|FD-Score:3.84|P-value:6.23E-5||SGD DESC:Sporulation protein required for prospore membrane formation at selected spindle poles, ensures functionality of all four spindle pole bodies during meiosis II; not required for meiotic recombination or meiotic chromosome segregation Gene:PHB2(YGR231C)|FD-Score:3.21|P-value:6.75E-4||SGD DESC:Subunit of the prohibitin complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation Gene:PIC2(YER053C)|FD-Score:-5.23|P-value:8.50E-8||SGD DESC:Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Mir1p but less abundant than Mir1p under normal conditions; expression is induced at high temperature Gene:PIN4(YBL051C)|FD-Score:-3.37|P-value:3.70E-4||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:POC4(YPL144W)|FD-Score:3.93|P-value:4.29E-5||SGD DESC:Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions Gene:PPH22(YDL188C)|FD-Score:3.17|P-value:7.73E-4||SGD DESC:Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph21p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; protein abundance increases in response to DNA replication stress; PPH22 has a paralog, PPH21, that arose from the whole genome duplication Gene:PPT2(YPL148C)|FD-Score:3.75|P-value:8.76E-5||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PRS4(YBL068W)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS4 has a paralog, PRS2, that arose from the whole genome duplication Gene:PTP3(YER075C)|FD-Score:4.05|P-value:2.59E-5||SGD DESC:Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the cytoplasm Gene:PUT2(YHR037W)|FD-Score:3.26|P-value:5.49E-4||SGD DESC:Delta-1-pyrroline-5-carboxylate dehydrogenase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of the human homolog causes HPII, an autosomal recessive inborn error of metabolism Gene:RFM1(YOR279C)|FD-Score:-3.3|P-value:4.80E-4||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RGL1(YPL066W)|FD-Score:-4.18|P-value:1.48E-5||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RIF2(YLR453C)|FD-Score:3.85|P-value:6.00E-5||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:RMD8(YFR048W)|FD-Score:5.33|P-value:4.95E-8||SGD DESC:Cytosolic protein required for sporulation Gene:ROX3(YBL093C)|FD-Score:3.31|P-value:4.68E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme Gene:RPL35B(YDL136W)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:11.4|P-value:1.59E-30||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS9B(YBR189W)|FD-Score:-4.93|P-value:4.13E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9B has a paralog, RPS9A, that arose from the whole genome duplication Gene:RSM24(YDR175C)|FD-Score:3.17|P-value:7.59E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:SET5(YHR207C)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus Gene:SFL1(YOR140W)|FD-Score:-3.76|P-value:8.50E-5||SGD DESC:Transcriptional repressor and activator; involved in repression of flocculation-related genes, and activation of stress responsive genes; negatively regulated by cAMP-dependent protein kinase A subunit Tpk2p Gene:SLI1(YGR212W)|FD-Score:3.81|P-value:6.89E-5||SGD DESC:N-acetyltransferase, confers resistance to the sphingolipid biosynthesis inhibitor myriocin (ISP-1) by converting it into N-acetyl-myriocin, co-operates with Ypk1p in mediating resistance to myriocin Gene:SOL4(YGR248W)|FD-Score:3.75|P-value:8.91E-5||SGD DESC:6-phosphogluconolactonase; protein abundance increases in response to DNA replication stress; SOL4 has a paralog, SOL3, that arose from the whole genome duplication Gene:SPR6(YER115C)|FD-Score:-4.62|P-value:1.92E-6||SGD DESC:Protein of unknown function, expressed during sporulation; not required for sporulation, but gene exhibits genetic interactions with other genes required for sporulation Gene:SRO77(YBL106C)|FD-Score:-3.97|P-value:3.53E-5||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:SUR4(YLR372W)|FD-Score:-3.75|P-value:8.72E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SWI3(YJL176C)|FD-Score:4.41|P-value:5.16E-6||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; SWI/SNF regulates transcription by remodeling chromosomes; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; relocates to the cytosol under hypoxic conditions Gene:TDH3(YGR192C)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; TDH3 has a paralog, TDH2, that arose from the whole genome duplication Gene:TRM13(YOL125W)|FD-Score:3.78|P-value:7.86E-5||SGD DESC:2'-O-methyltransferase responsible for modification of tRNA at position 4; C-terminal domain has similarity to Rossmann-fold (RFM) superfamily of RNA methyltransferases Gene:TRP1(YDR007W)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TYW1(YPL207W)|FD-Score:6.36|P-value:1.00E-10||SGD DESC:Iron-sulfer protein required for synthesis of Wybutosine modified tRNA; Wybutosine is a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions; induction by Yap5p in response to iron provides protection from high iron toxicity; overexpression results in increased cellular iron Gene:URE2(YNL229C)|FD-Score:-4.11|P-value:1.97E-5||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:URM1(YIL008W)|FD-Score:4.45|P-value:4.33E-6||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:USA1(YML029W)|FD-Score:3.75|P-value:8.74E-5||SGD DESC:Scaffold subunit of the Hrd1p ubiquitin ligase that also promotes ligase oligomerization; involved in ER-associated protein degradation (ERAD); interacts with the U1 snRNP-specific protein, Snp1p Gene:VPS15(YBR097W)|FD-Score:-4.14|P-value:1.75E-5||SGD DESC:Serine/threonine protein kinase involved in vacuolar protein sorting; functions as a membrane-associated complex with Vps34p; active form recruits Vps34p to the Golgi membrane; interacts with the GDP-bound form of Gpa1p; myristoylated; a fraction is localized, with Vps34p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery Gene:YAL004W(YAL004W_d)|FD-Score:3.32|P-value:4.44E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene SSA1/YAL005C Gene:YAL018C(YAL018C_p)|FD-Score:-3.58|P-value:1.73E-4||SGD DESC:Putative protein of unknown function Gene:YAL056C-A(YAL056C-A_d)|FD-Score:-4.08|P-value:2.23E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YAL067W-A(YAL067W-A_p)|FD-Score:6.13|P-value:4.40E-10||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR226C(YBR226C_d)|FD-Score:-3.99|P-value:3.30E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W Gene:YCL046W(YCL046W_d)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C Gene:YCR025C(YCR025C_d)|FD-Score:4.36|P-value:6.64E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR025C is not an essential gene Gene:YDL026W(YDL026W_d)|FD-Score:-4.03|P-value:2.76E-5||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR090C(YDR090C_p)|FD-Score:-3.84|P-value:6.15E-5||SGD DESC:Putative protein of unknown function Gene:YEA6(YEL006W)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Putative mitochondrial NAD+ transporter, member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog Gene:YER158C(YER158C_p)|FD-Score:5.21|P-value:9.41E-8||SGD DESC:Protein of unknown function; potentially phosphorylated by Cdc28p; YER158C has a paralog, AFR1, that arose from the whole genome duplication Gene:YET1(YKL065C)|FD-Score:4.37|P-value:6.16E-6||SGD DESC:Endoplasmic reticulum transmembrane protein; may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein Gene:YGL006W-A(YGL006W-A_p)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YGR122W(YGR122W)|FD-Score:3.99|P-value:3.25E-5||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YHR007C-A(YHR007C-A_p)|FD-Score:3.85|P-value:5.96E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YIL166C(YIL166C_p)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Putative protein with similarity to the allantoate permease (Dal5p) subfamily of the major facilitator superfamily; mRNA expression is elevated by sulfur limitation; YIL166C is a non-essential gene Gene:YLL020C(YLL020C_d)|FD-Score:4.78|P-value:8.58E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KNS1 Gene:YLR255C(YLR255C_d)|FD-Score:-3.18|P-value:7.44E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR283W(YLR283W_p)|FD-Score:4.96|P-value:3.49E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR283W is not an essential gene Gene:YML003W(YML003W_p)|FD-Score:-4.3|P-value:8.69E-6||SGD DESC:Putative protein of unknown function Gene:YNL050C(YNL050C_p)|FD-Score:-4.49|P-value:3.51E-6||SGD DESC:Putative protein of unknown function; YNL050c is not an essential gene Gene:YNL058C(YNL058C_p)|FD-Score:3.36|P-value:3.92E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YNL058C is not an essential gene Gene:YPT11(YNL304W)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Rab family GTPase that interacts with the C-terminal tail domain of Myo2p; mediates distribution of mitochondria and endoplasmic reticuli to daughter cells Gene:ABZ1(YNR033W)|FD-Score:3.11|P-value:9.43E-4||SGD DESC:Para-aminobenzoate (PABA) synthase; has similarity to Escherichia coli PABA synthase components PabA and PabB; required for the synthesis of para-aminobenzoic acid, an important intermediate for folate and ubiquinone Q biosynthesis; protein abundance increases in response to DNA replication stress Gene:ACE2(YLR131C)|FD-Score:4.55|P-value:2.65E-6||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:AFT2(YPL202C)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; AFT2 has a paralog, AFT1, that arose from the whole genome duplication Gene:ALP1(YNL270C)|FD-Score:6.13|P-value:4.29E-10||SGD DESC:Arginine transporter; expression is normally very low and it is unclear what conditions would induce significant expression; ALP1 has a paralog, CAN1, that arose from the whole genome duplication Gene:AMA1(YGR225W)|FD-Score:4.06|P-value:2.40E-5||SGD DESC:Activator of meiotic anaphase promoting complex (APC/C); Cdc20p family member; required for initiation of spore wall assembly; required for Clb1p degradation during meiosis Gene:APE2(YKL157W)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Aminopeptidase yscII; may have a role in obtaining leucine from dipeptide substrates; APE2 has a paralog, AAP1, that arose from the whole genome duplication Gene:ARP1(YHR129C)|FD-Score:4.46|P-value:4.03E-6||SGD DESC:Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin Gene:AST1(YBL069W)|FD-Score:3.14|P-value:8.46E-4||SGD DESC:Lipid raft associated protein; interacts with the plasma membrane ATPase Pma1p and has a role in its targeting to the plasma membrane by influencing its incorporation into lipid rafts; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST1 has a paralog, AST2, that arose from the whole genome duplication Gene:ATS1(YAL020C)|FD-Score:4.57|P-value:2.42E-6||SGD DESC:Protein required, with Elongator complex, Kti11p, and Kti12p, for modification of wobble nucleosides in tRNA; has a potential role in regulatory interactions between microtubules and the cell cycle Gene:BRE5(YNR051C)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A Gene:CPA1(YOR303W)|FD-Score:-3.45|P-value:2.77E-4||SGD DESC:Small subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor; translationally regulated by an attenuator peptide encoded by YOR302W within the CPA1 mRNA 5'-leader Gene:DDI1(YER143W)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:DNA damage-inducible v-SNARE binding protein with a role in suppression of protein secretion; may play a role in S-phase checkpoint control; has ubiquitin-associated (UBA), ubiquitin-like (UBL), and retroviral-like proteinase (RVP) domains Gene:DEP1(YAL013W)|FD-Score:3.34|P-value:4.25E-4||SGD DESC:Transcriptional modulator involved in regulation of structural phospholipid biosynthesis genes and metabolically unrelated genes, as well as maintenance of telomeres, mating efficiency, and sporulation Gene:DFG16(YOR030W)|FD-Score:3.11|P-value:9.25E-4||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:ENV10(YLR065C)|FD-Score:3.33|P-value:4.33E-4||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:FEN2(YCR028C)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:FET4(YMR319C)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FMP21(YBR269C_p)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GDH1(YOR375C)|FD-Score:4.56|P-value:2.62E-6||SGD DESC:NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh3p; expression regulated by nitrogen and carbon sources; GDH1 has a paralog, GDH3, that arose from the whole genome duplication Gene:GUT1(YHL032C)|FD-Score:14|P-value:1.44E-44||SGD DESC:Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p Gene:GYP1(YOR070C)|FD-Score:3.16|P-value:7.89E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HDA2(YDR295C)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex containing an Hda1p homodimer and an Hda2p-Hda3p heterodimer; involved in telomere maintenance Gene:ICT1(YLR099C)|FD-Score:6.34|P-value:1.15E-10||SGD DESC:Lysophosphatidic acid acyltransferase; responsible for enhanced phospholipid synthesis during organic solvent stress; null displays increased sensitivity to Calcofluor white; highly expressed during organic solvent stress; ICT1 has a paralog, ECM18, that arose from the whole genome duplication Gene:IRC25(YLR021W)|FD-Score:3.1|P-value:9.82E-4||SGD DESC:Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions Gene:IRS4(YKR019C)|FD-Score:3.19|P-value:7.03E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LAC1(YKL008C)|FD-Score:-5.41|P-value:3.10E-8||SGD DESC:Ceramide synthase component, involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lag1p Gene:LEA1(YPL213W)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Component of U2 snRNP; disruption causes reduced U2 snRNP levels; physically interacts with Msl1p; putative homolog of human U2A' snRNP protein Gene:LRP1(YHR081W)|FD-Score:-4.07|P-value:2.33E-5||SGD DESC:Nuclear exosome-associated nucleic acid binding protein; involved in RNA processing, surveillance, degradation, tethering, and export; homolog of mammalian nuclear matrix protein C1D involved in regulation of DNA repair and recombination Gene:MMS22(YLR320W)|FD-Score:-3.2|P-value:6.95E-4||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork, such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; required for accurate meiotic chromosome segregation Gene:MMT2(YPL224C)|FD-Score:4.48|P-value:3.74E-6||SGD DESC:Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt1p Gene:MNN10(YDR245W)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family Gene:MSH6(YDR097C)|FD-Score:-3.79|P-value:7.55E-5||SGD DESC:Protein required for mismatch repair in mitosis and meiosis, forms a complex with Msh2p to repair both single-base & insertion-deletion mispairs; also involved in interstrand cross-link repair; potentially phosphorylated by Cdc28p Gene:OMS1(YDR316W)|FD-Score:3.82|P-value:6.75E-5||SGD DESC:Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations Gene:PFS1(YHR185C)|FD-Score:3.84|P-value:6.23E-5||SGD DESC:Sporulation protein required for prospore membrane formation at selected spindle poles, ensures functionality of all four spindle pole bodies during meiosis II; not required for meiotic recombination or meiotic chromosome segregation Gene:PHB2(YGR231C)|FD-Score:3.21|P-value:6.75E-4||SGD DESC:Subunit of the prohibitin complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation Gene:PIC2(YER053C)|FD-Score:-5.23|P-value:8.50E-8||SGD DESC:Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Mir1p but less abundant than Mir1p under normal conditions; expression is induced at high temperature Gene:PIN4(YBL051C)|FD-Score:-3.37|P-value:3.70E-4||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:POC4(YPL144W)|FD-Score:3.93|P-value:4.29E-5||SGD DESC:Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions Gene:PPH22(YDL188C)|FD-Score:3.17|P-value:7.73E-4||SGD DESC:Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph21p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; protein abundance increases in response to DNA replication stress; PPH22 has a paralog, PPH21, that arose from the whole genome duplication Gene:PPT2(YPL148C)|FD-Score:3.75|P-value:8.76E-5||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PRS4(YBL068W)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS4 has a paralog, PRS2, that arose from the whole genome duplication Gene:PTP3(YER075C)|FD-Score:4.05|P-value:2.59E-5||SGD DESC:Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the cytoplasm Gene:PUT2(YHR037W)|FD-Score:3.26|P-value:5.49E-4||SGD DESC:Delta-1-pyrroline-5-carboxylate dehydrogenase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of the human homolog causes HPII, an autosomal recessive inborn error of metabolism Gene:RFM1(YOR279C)|FD-Score:-3.3|P-value:4.80E-4||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RGL1(YPL066W)|FD-Score:-4.18|P-value:1.48E-5||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RIF2(YLR453C)|FD-Score:3.85|P-value:6.00E-5||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:RMD8(YFR048W)|FD-Score:5.33|P-value:4.95E-8||SGD DESC:Cytosolic protein required for sporulation Gene:ROX3(YBL093C)|FD-Score:3.31|P-value:4.68E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme Gene:RPL35B(YDL136W)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:11.4|P-value:1.59E-30||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS9B(YBR189W)|FD-Score:-4.93|P-value:4.13E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9B has a paralog, RPS9A, that arose from the whole genome duplication Gene:RSM24(YDR175C)|FD-Score:3.17|P-value:7.59E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:SET5(YHR207C)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus Gene:SFL1(YOR140W)|FD-Score:-3.76|P-value:8.50E-5||SGD DESC:Transcriptional repressor and activator; involved in repression of flocculation-related genes, and activation of stress responsive genes; negatively regulated by cAMP-dependent protein kinase A subunit Tpk2p Gene:SLI1(YGR212W)|FD-Score:3.81|P-value:6.89E-5||SGD DESC:N-acetyltransferase, confers resistance to the sphingolipid biosynthesis inhibitor myriocin (ISP-1) by converting it into N-acetyl-myriocin, co-operates with Ypk1p in mediating resistance to myriocin Gene:SOL4(YGR248W)|FD-Score:3.75|P-value:8.91E-5||SGD DESC:6-phosphogluconolactonase; protein abundance increases in response to DNA replication stress; SOL4 has a paralog, SOL3, that arose from the whole genome duplication Gene:SPR6(YER115C)|FD-Score:-4.62|P-value:1.92E-6||SGD DESC:Protein of unknown function, expressed during sporulation; not required for sporulation, but gene exhibits genetic interactions with other genes required for sporulation Gene:SRO77(YBL106C)|FD-Score:-3.97|P-value:3.53E-5||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:SUR4(YLR372W)|FD-Score:-3.75|P-value:8.72E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SWI3(YJL176C)|FD-Score:4.41|P-value:5.16E-6||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; SWI/SNF regulates transcription by remodeling chromosomes; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; relocates to the cytosol under hypoxic conditions Gene:TDH3(YGR192C)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; TDH3 has a paralog, TDH2, that arose from the whole genome duplication Gene:TRM13(YOL125W)|FD-Score:3.78|P-value:7.86E-5||SGD DESC:2'-O-methyltransferase responsible for modification of tRNA at position 4; C-terminal domain has similarity to Rossmann-fold (RFM) superfamily of RNA methyltransferases Gene:TRP1(YDR007W)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TYW1(YPL207W)|FD-Score:6.36|P-value:1.00E-10||SGD DESC:Iron-sulfer protein required for synthesis of Wybutosine modified tRNA; Wybutosine is a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions; induction by Yap5p in response to iron provides protection from high iron toxicity; overexpression results in increased cellular iron Gene:URE2(YNL229C)|FD-Score:-4.11|P-value:1.97E-5||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:URM1(YIL008W)|FD-Score:4.45|P-value:4.33E-6||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:USA1(YML029W)|FD-Score:3.75|P-value:8.74E-5||SGD DESC:Scaffold subunit of the Hrd1p ubiquitin ligase that also promotes ligase oligomerization; involved in ER-associated protein degradation (ERAD); interacts with the U1 snRNP-specific protein, Snp1p Gene:VPS15(YBR097W)|FD-Score:-4.14|P-value:1.75E-5||SGD DESC:Serine/threonine protein kinase involved in vacuolar protein sorting; functions as a membrane-associated complex with Vps34p; active form recruits Vps34p to the Golgi membrane; interacts with the GDP-bound form of Gpa1p; myristoylated; a fraction is localized, with Vps34p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery Gene:YAL004W(YAL004W_d)|FD-Score:3.32|P-value:4.44E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene SSA1/YAL005C Gene:YAL018C(YAL018C_p)|FD-Score:-3.58|P-value:1.73E-4||SGD DESC:Putative protein of unknown function Gene:YAL056C-A(YAL056C-A_d)|FD-Score:-4.08|P-value:2.23E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YAL067W-A(YAL067W-A_p)|FD-Score:6.13|P-value:4.40E-10||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR226C(YBR226C_d)|FD-Score:-3.99|P-value:3.30E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W Gene:YCL046W(YCL046W_d)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C Gene:YCR025C(YCR025C_d)|FD-Score:4.36|P-value:6.64E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR025C is not an essential gene Gene:YDL026W(YDL026W_d)|FD-Score:-4.03|P-value:2.76E-5||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR090C(YDR090C_p)|FD-Score:-3.84|P-value:6.15E-5||SGD DESC:Putative protein of unknown function Gene:YEA6(YEL006W)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Putative mitochondrial NAD+ transporter, member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog Gene:YER158C(YER158C_p)|FD-Score:5.21|P-value:9.41E-8||SGD DESC:Protein of unknown function; potentially phosphorylated by Cdc28p; YER158C has a paralog, AFR1, that arose from the whole genome duplication Gene:YET1(YKL065C)|FD-Score:4.37|P-value:6.16E-6||SGD DESC:Endoplasmic reticulum transmembrane protein; may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein Gene:YGL006W-A(YGL006W-A_p)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YGR122W(YGR122W)|FD-Score:3.99|P-value:3.25E-5||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YHR007C-A(YHR007C-A_p)|FD-Score:3.85|P-value:5.96E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YIL166C(YIL166C_p)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Putative protein with similarity to the allantoate permease (Dal5p) subfamily of the major facilitator superfamily; mRNA expression is elevated by sulfur limitation; YIL166C is a non-essential gene Gene:YLL020C(YLL020C_d)|FD-Score:4.78|P-value:8.58E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KNS1 Gene:YLR255C(YLR255C_d)|FD-Score:-3.18|P-value:7.44E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR283W(YLR283W_p)|FD-Score:4.96|P-value:3.49E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR283W is not an essential gene Gene:YML003W(YML003W_p)|FD-Score:-4.3|P-value:8.69E-6||SGD DESC:Putative protein of unknown function Gene:YNL050C(YNL050C_p)|FD-Score:-4.49|P-value:3.51E-6||SGD DESC:Putative protein of unknown function; YNL050c is not an essential gene Gene:YNL058C(YNL058C_p)|FD-Score:3.36|P-value:3.92E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YNL058C is not an essential gene Gene:YPT11(YNL304W)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Rab family GTPase that interacts with the C-terminal tail domain of Myo2p; mediates distribution of mitochondria and endoplasmic reticuli to daughter cells

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YER022W9.441.79E-214.25SRB4Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination
YOL077C5.191.04E-70.03BRX1Nucleolar protein, constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif
YOR095C5.161.21E-70.10RKI1Ribose-5-phosphate ketol-isomerase, catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis
YDR187C_d5.062.08E-70.04YDR187C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W
YLR010C5.022.58E-70.11TEN1Protein that regulates telomeric length; protects telomeric ends in a complex with Cdc13p and Stn1p
YJR022W4.914.48E-70.90LSM8Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) that is part of spliceosomal U6 snRNP and is also implicated in processing of pre-tRNA, pre-snoRNA, and pre-rRNA
YOR319W4.013.00E-50.01HSH49U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM)
YPL218W4.013.09E-50.08SAR1GTPase, GTP-binding protein of the ARF family, component of COPII coat of vesicles; required for transport vesicle formation during ER to Golgi protein transport
YKL196C3.924.40E-50.23YKT6Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus
YLL011W3.691.13E-40.05SOF1Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p
YDL195W3.641.38E-40.02SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YPR169W3.621.50E-40.19JIP5Essential protein required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein
YJR067C3.423.09E-40.08YAE1Protein that forms a complex with Lto1p and Rli1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species
YHR062C3.344.12E-40.05RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YPR136C_d3.295.00E-40.11YPR136C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YHL032C14.001.44E-44GUT1Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p
YDL020C11.401.59E-30RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YPL207W6.361.00E-10TYW1Iron-sulfer protein required for synthesis of Wybutosine modified tRNA; Wybutosine is a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions; induction by Yap5p in response to iron provides protection from high iron toxicity; overexpression results in increased cellular iron
YLR099C6.341.15E-10ICT1Lysophosphatidic acid acyltransferase; responsible for enhanced phospholipid synthesis during organic solvent stress; null displays increased sensitivity to Calcofluor white; highly expressed during organic solvent stress; ICT1 has a paralog, ECM18, that arose from the whole genome duplication
YNL270C6.134.29E-10ALP1Arginine transporter; expression is normally very low and it is unclear what conditions would induce significant expression; ALP1 has a paralog, CAN1, that arose from the whole genome duplication
YAL067W-A_p6.134.40E-10YAL067W-A_pPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
YFR048W5.334.95E-8RMD8Cytosolic protein required for sporulation
YER158C_p5.219.41E-8YER158C_pProtein of unknown function; potentially phosphorylated by Cdc28p; YER158C has a paralog, AFR1, that arose from the whole genome duplication
YLR283W_p4.963.49E-7YLR283W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR283W is not an essential gene
YLL020C_d4.788.58E-7YLL020C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KNS1
YAL020C4.572.42E-6ATS1Protein required, with Elongator complex, Kti11p, and Kti12p, for modification of wobble nucleosides in tRNA; has a potential role in regulatory interactions between microtubules and the cell cycle
YOR375C4.562.62E-6GDH1NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh3p; expression regulated by nitrogen and carbon sources; GDH1 has a paralog, GDH3, that arose from the whole genome duplication
YLR131C4.552.65E-6ACE2Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication
YPL224C4.483.74E-6MMT2Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt1p
YHR129C4.464.03E-6ARP1Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin

GO enrichment analysis for SGTC_2787
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1702.41E-39SGTC_26602',4'-dihydroxychalcone 17.1 μMTimTec (Pure natural product library)53572180.12069
0.1254.80E-22SGTC_14173966-0327 90.4 μMChemDiv (Drug-like library)6511180.0675676
0.1232.15E-21SGTC_26682',4'-dihydroxychalcone 15.1 μMTimTec (Pure natural product library)53572180.12069
0.1193.93E-20SGTC_23859074692 200.0 μMChembridge (Fragment library)170469060.0943396
0.1187.24E-20SGTC_32799140807 49.5 μMChembridge (Drug-like library)455957180.122807
0.1171.77E-19SGTC_1165k072-0275 30.3 μMChemDiv (Drug-like library)61535230.0952381
0.1124.86E-18SGTC_2701st077767 14.7 μMTimTec (Natural product derivative library)57211880.126984
0.1063.40E-16SGTC_2499avocadenofuran 51.5 μMMicrosource (Natural product library)68577920.0677966
0.1017.39E-15SGTC_31699102970 49.5 μMChembridge (Drug-like library)170278390.107692RPP1 & pyrimidine depletion
0.0992.87E-14SGTC_7033875-0075 71.8 μMChemDiv (Drug-like library)28508140.142857
0.0961.78E-13SGTC_1026k007-1105 30.6 μMChemDiv (Drug-like library)70667940.177419ERAD & cell cycle
0.0943.49E-13SGTC_577r071-0003 38.6 μMChemDiv (Drug-like library)45685170.12963fatty acid desaturase (OLE1)
0.0912.02E-12SGTC_23127706453 155.7 μMChembridge (Fragment library)9760980.130435
0.0912.67E-12SGTC_395anisomycin 6.2 μMICCB bioactive library66103090.140625
0.0891.02E-11SGTC_2235salicylic acid 500.0 μMMiscellaneous3380.145833

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_3176910658249.47 μM0.80487817018027Chembridge (Drug-like library)275.730163.74812
SGTC_2900532852885.29 μM0.651163795359Chembridge (Drug-like library)261.703583.26212Golgi
SGTC_3205911348349.47 μM0.58695717018033Chembridge (Drug-like library)271.311083.06813
SGTC_8261683-5028118 μM0.577778722377ChemDiv (Drug-like library)261.703583.26212
SGTC_3203911354349.47 μM0.56818217018012Chembridge (Drug-like library)241.28513.08412
SGTC_12550566-0045221 μM0.5434782788469ChemDiv (Drug-like library)304.728323.04223excess fatty acid
SGTC_7161683-5115658 μM0.541667723597ChemDiv (Drug-like library)303.740263.48313
SGTC_3188911078949.47 μM0.541667849217Chembridge (Drug-like library)275.730163.61112iron homeostasis
SGTC_3186911046449.47 μM0.52173917018021Chembridge (Drug-like library)269.338264.05612RPP1 & pyrimidine depletion
SGTC_2867903909871.43 μM0.4807696463460Chembridge (Drug-like library)287.740863.87912iron homeostasis