1273-0055

2-(3-hexadecylimidazol-1-ium-1-yl)acetonitrile

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_279
Screen concentration 2.9 μM
Source ChemDiv (Drug-like library)
PubChem CID 2847562
SMILES CCCCCCCCCCCCCCCCN1C=C[N+](=C1)CC#N
Standardized SMILES CCCCCCCCCCCCCCCCn1cc[n+](CC#N)c1
Molecular weight 332.5465
ALogP 7.8
H-bond donor count 0
H-bond acceptor count 1
Response signature azole & statin

Pool Growth Kinetics
% growth inhibition (Het. pool) 12.92
% growth inhibition (Hom. pool) 13.67


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2847562
Download HIP data (tab-delimited text)  (excel)
Gene:ACS2(YLR153C)|FD-Score:4.02|P-value:2.86E-5|Clearance:0.69||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions Gene:DSN1(YIR010W)|FD-Score:3.33|P-value:4.35E-4|Clearance:0.09||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; important for chromosome segregation Gene:ECM9(YKR004C)|FD-Score:3.24|P-value:5.95E-4|Clearance:0.02||SGD DESC:Non-essential protein of unknown function Gene:ERG7(YHR072W)|FD-Score:3.22|P-value:6.40E-4|Clearance:0.24||SGD DESC:Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis Gene:NOC4(YPR144C)|FD-Score:-3.27|P-value:5.45E-4|Clearance:0||SGD DESC:Nucleolar protein, forms a complex with Nop14p that mediates maturation and nuclear export of 40S ribosomal subunits Gene:SMC4(YLR086W)|FD-Score:-4.02|P-value:2.91E-5|Clearance:0||SGD DESC:Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate Gene:YOL134C(YOL134C_d)|FD-Score:4.29|P-value:9.12E-6|Clearance:0.69||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps HRT1, a verified gene that encodes an SCF ubiquitin ligase subunit Gene:ACS2(YLR153C)|FD-Score:4.02|P-value:2.86E-5|Clearance:0.69||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions Gene:DSN1(YIR010W)|FD-Score:3.33|P-value:4.35E-4|Clearance:0.09||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; important for chromosome segregation Gene:ECM9(YKR004C)|FD-Score:3.24|P-value:5.95E-4|Clearance:0.02||SGD DESC:Non-essential protein of unknown function Gene:ERG7(YHR072W)|FD-Score:3.22|P-value:6.40E-4|Clearance:0.24||SGD DESC:Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis Gene:NOC4(YPR144C)|FD-Score:-3.27|P-value:5.45E-4|Clearance:0||SGD DESC:Nucleolar protein, forms a complex with Nop14p that mediates maturation and nuclear export of 40S ribosomal subunits Gene:SMC4(YLR086W)|FD-Score:-4.02|P-value:2.91E-5|Clearance:0||SGD DESC:Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate Gene:YOL134C(YOL134C_d)|FD-Score:4.29|P-value:9.12E-6|Clearance:0.69||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps HRT1, a verified gene that encodes an SCF ubiquitin ligase subunit

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2847562
Download HOP data (tab-delimited text)  (excel)
Gene:AIM22(YJL046W)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Putative lipoate-protein ligase, required along with Lip2 and Lip5 for lipoylation of Lat1p and Kgd2p; similar to E. coli LplA; null mutant displays reduced frequency of mitochondrial genome loss Gene:APE4(YHR113W)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Cytoplasmic aspartyl aminopeptidase that may also function in the vacuole; CVT pathway cargo protein; cleaves unblocked N-terminal acidic amino acids from peptide substrates; forms a 12-subunit homo-oligomer; M18 metalloprotease family Gene:ARO1(YDR127W)|FD-Score:6.13|P-value:4.31E-10||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO7(YPR060C)|FD-Score:4.84|P-value:6.36E-7||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:ATG32(YIL146C)|FD-Score:3.22|P-value:6.32E-4||SGD DESC:Mitochondrial outer membrane protein required to initiate mitophagy; recruits the autophagy adaptor protein Atg11p and the ubiquitin-like protein Atg8p to the mitochondrial surface to initiate mitophagy, the selective vacuolar degradation of mitochondria in response to starvation; can promote pexophagy when placed ectopically in the peroxisomal membrane Gene:CEM1(YER061C)|FD-Score:7.05|P-value:9.17E-13||SGD DESC:Mitochondrial beta-keto-acyl synthase with possible role in fatty acid synthesis; required for mitochondrial respiration Gene:CHS5(YLR330W)|FD-Score:5.75|P-value:4.34E-9||SGD DESC:Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane Gene:COX7(YMR256C)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CYS3(YAL012W)|FD-Score:5.97|P-value:1.16E-9||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:CYS4(YGR155W)|FD-Score:6.09|P-value:5.82E-10||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:DAL81(YIR023W)|FD-Score:5.89|P-value:1.96E-9||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DFG16(YOR030W)|FD-Score:6.25|P-value:2.07E-10||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:DSE2(YHR143W)|FD-Score:4.75|P-value:1.01E-6||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:EAF3(YPR023C)|FD-Score:4.35|P-value:6.89E-6||SGD DESC:Esa1p-associated factor, nonessential component of the NuA4 acetyltransferase complex, homologous to Drosophila dosage compensation protein MSL3; plays a role in regulating Ty1 transposition Gene:ECM22(YLR228C)|FD-Score:3.29|P-value:4.97E-4||SGD DESC:Sterol regulatory element binding protein; regulates transcription of sterol biosynthetic genes; contains Zn[2]-Cys[6] binuclear cluster; relocates from intracellular membranes to perinuclear foci on sterol depletion; ECM22 has a paralog, UPC2, that arose from the whole genome duplication Gene:ECT1(YGR007W)|FD-Score:4.53|P-value:2.98E-6||SGD DESC:Ethanolamine-phosphate cytidylyltransferase, catalyzes the second step of phosphatidylethanolamine biosynthesis; involved in the maintenance of plasma membrane; similar to mammalian CTP: phosphocholine cytidylyl-transferases Gene:ERG2(YMR202W)|FD-Score:5.26|P-value:7.04E-8||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ETT1(YOR051C)|FD-Score:4.1|P-value:2.04E-5||SGD DESC:Nuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts; deletion increases stop codon readthrough Gene:FIS1(YIL065C)|FD-Score:3.34|P-value:4.18E-4||SGD DESC:Protein involved in mitochondrial membrane fission and peroxisome abundance; required for localization of Dnm1p and Mdv1p during mitochondrial division; mediates ethanol-induced apoptosis and ethanol-induced mitochondrial fragmentation Gene:FZO1(YBR179C)|FD-Score:3.75|P-value:8.74E-5||SGD DESC:Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system Gene:GCN4(YEL009C)|FD-Score:4.28|P-value:9.19E-6||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GEM1(YAL048C)|FD-Score:3.1|P-value:9.80E-4||SGD DESC:Outer mitochondrial membrane GTPase, subunit of the ERMES complex; potential regulatory subunit of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth; cells lacking Gem1p contain collapsed, globular, or grape-like mitochondria; ortholog of metazoan Miro GTPases Gene:GIS3(YLR094C)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Protein of unknown function Gene:GPB2(YAL056W)|FD-Score:3.23|P-value:6.16E-4||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; inhibits Ras activity through direct interactions with Ira1p/2p; regulated by G-alpha protein Gpa2p; GPB2 has a paralog, GPB1, that arose from the whole genome duplication Gene:GUD1(YDL238C)|FD-Score:-3.64|P-value:1.38E-4||SGD DESC:Guanine deaminase, a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures Gene:HMG1(YML075C)|FD-Score:8.66|P-value:2.45E-18||SGD DESC:HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress Gene:HMG2(YLR450W)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:HMG-CoA reductase; converts HMG-CoA to mevalonate, a rate-limiting step in sterol biosynthesis; one of two isozymes; overproduction induces assembly of peripheral ER membrane arrays and short nuclear-associated membrane stacks; forms foci at the nuclear periphery upon DNA replication stress Gene:HOS2(YGL194C)|FD-Score:8.07|P-value:3.44E-16||SGD DESC:Histone deacetylase and subunit of Set3 and Rpd3L complexes; required for gene activation via specific deacetylation of lysines in H3 and H4 histone tails; subunit of the Set3 complex, a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; co-localizes with Cmr1p in nuclear foci in response to DNA damage by MMS Gene:HTD2(YHR067W)|FD-Score:3.78|P-value:7.71E-5||SGD DESC:Mitochondrial 3-hydroxyacyl-thioester dehydratase involved in fatty acid biosynthesis, required for respiratory growth and for normal mitochondrial morphology Gene:IMP2'(YIL154C)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:IRE1(YHR079C)|FD-Score:3.12|P-value:9.17E-4||SGD DESC:Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress Gene:LCL1(YPL056C_p)|FD-Score:-3.09|P-value:0.00100||SGD DESC:Putative protein of unknown function; deletion mutant is fluconazole resistant and has long chronological lifespan Gene:LIP2(YLR239C)|FD-Score:3.96|P-value:3.72E-5||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:LIP5(YOR196C)|FD-Score:5.42|P-value:3.05E-8||SGD DESC:Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthase Gene:LPD1(YFL018C)|FD-Score:5.37|P-value:4.05E-8||SGD DESC:Dihydrolipoamide dehydrogenase, the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multi-enzyme complexes Gene:MIP1(YOR330C)|FD-Score:3.54|P-value:1.96E-4||SGD DESC:Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit Gene:MRPL13(YKR006C)|FD-Score:3.24|P-value:6.01E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MRPL33(YMR286W)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRS3(YJL133W)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Iron transporter, mediates Fe2+ transport across inner mito membrane; mitochondrial carrier family member; active under low-iron conditions; may transport other cations; MRS3 has a paralog, MRS4, that arose from the whole genome duplication Gene:MRS4(YKR052C)|FD-Score:5.5|P-value:1.92E-8||SGD DESC:Iron transporter of the mitochondrial carrier family; mediates Fe2+ transport across the inner mitochondrial membrane; active under low-iron conditions; may transport other cations; protein abundance increases in response to DNA replication stress; MRS4 has a paralog, MRS3, that arose from the whole genome duplication Gene:MST1(YKL194C)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:MTM1(YGR257C)|FD-Score:6.95|P-value:1.81E-12||SGD DESC:Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor Gene:NFU1(YKL040C)|FD-Score:3.1|P-value:9.75E-4||SGD DESC:Protein involved in iron metabolism in mitochondria; similar to NifU, which is a protein required for the maturation of the Fe/S clusters of nitrogenase in nitrogen-fixing bacteria Gene:OAR1(YKL055C)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a type II mitochondrial fatty acid synthase along with Mct1p Gene:PDB1(YBR221C)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved multi-protein complex found in mitochondria Gene:PET123(YOR158W)|FD-Score:4.65|P-value:1.65E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator Gene:PGD1(YGL025C)|FD-Score:-3.39|P-value:3.44E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PIN4(YBL051C)|FD-Score:4.48|P-value:3.69E-6||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:RIM101(YHL027W)|FD-Score:8.21|P-value:1.08E-16||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RIM13(YMR154C)|FD-Score:6.72|P-value:9.38E-12||SGD DESC:Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB Gene:RIM20(YOR275C)|FD-Score:5.04|P-value:2.32E-7||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation Gene:RIM21(YNL294C)|FD-Score:4.38|P-value:5.80E-6||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RIM8(YGL045W)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:RML2(YEL050C)|FD-Score:3.19|P-value:7.02E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:SAC6(YDR129C)|FD-Score:-3.13|P-value:8.77E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SAS2(YMR127C)|FD-Score:4.11|P-value:1.97E-5||SGD DESC:Histone acetyltransferase (HAT) catalytic subunit of the SAS complex (Sas2p-Sas4p-Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; member of the MYSTacetyltransferase family Gene:SET3(YKR029C)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Defining member of the SET3 histone deacetylase complex which is a meiosis-specific repressor of sporulation genes; necessary for efficient transcription by RNAPII; one of two yeast proteins that contains both SET and PHD domains; SET3 has a paralog, SET4, that arose from the whole genome duplication Gene:SGE1(YPR198W)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SLA1(YBL007C)|FD-Score:4.88|P-value:5.30E-7||SGD DESC:Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains Gene:SNT1(YCR033W)|FD-Score:6.77|P-value:6.27E-12||SGD DESC:Subunit of the Set3C deacetylase complex that interacts directly with the Set3C subunit, Sif2p; putative DNA-binding protein; mutant has increased aneuploidy tolerance Gene:SPF1(YEL031W)|FD-Score:8.16|P-value:1.63E-16||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SPT3(YDR392W)|FD-Score:-3.2|P-value:6.79E-4||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SRB5(YGR104C)|FD-Score:3.75|P-value:8.98E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance Gene:THI2(YBR240C)|FD-Score:3.18|P-value:7.35E-4||SGD DESC:Transcriptional activator of thiamine biosynthetic genes; interacts with regulatory factor Thi3p to control expression of thiamine biosynthetic genes with respect to thiamine availability; acts together with Pdc2p to respond to thiaminediphosphate demand, possibly as related to carbon source availability; zinc finger protein of the Zn(II)2Cys6 type Gene:THR4(YCR053W)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TOM5(YPR133W-A)|FD-Score:4.6|P-value:2.09E-6||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import of all mitochondrially directed proteins; involved in transfer of precursors from the Tom70p and Tom20p receptors to the Tom40p pore Gene:TRP3(YKL211C)|FD-Score:3.31|P-value:4.69E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UTR1(YJR049C)|FD-Score:6.44|P-value:5.91E-11||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:YAP3(YHL009C)|FD-Score:4.11|P-value:1.99E-5||SGD DESC:Basic leucine zipper (bZIP) transcription factor Gene:YBL029C-A(YBL029C-A_p)|FD-Score:4.79|P-value:8.40E-7||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress; has potential orthologs in Saccharomyces species and in Yarrowia lipolytica Gene:YCL046W(YCL046W_d)|FD-Score:5.92|P-value:1.62E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C Gene:YDL023C(YDL023C_d)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in other Saccharomyces species; overlaps the verified gene GPD1; deletion confers sensitivity to GSAO; deletion in cyr1 mutant results in loss of stress resistance Gene:YGR122W(YGR122W)|FD-Score:6.54|P-value:3.17E-11||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YIA6(YIL006W)|FD-Score:3.68|P-value:1.19E-4||SGD DESC:Mitochondrial NAD+ transporter, involved in the transport of NAD+ into the mitochondria (see also YEA6); member of the mitochondrial carrier subfamily; disputed role as a pyruvate transporter; has putative mouse and human orthologs Gene:YLR118C(YLR118C)|FD-Score:4.01|P-value:2.99E-5||SGD DESC:Acyl-protein thioesterase responsible for depalmitoylation of Gpa1p; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS Gene:YML007C-A(YML007C-A_p)|FD-Score:4.45|P-value:4.31E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YNL042W-B(YNL042W-B_p)|FD-Score:3.22|P-value:6.52E-4||SGD DESC:Putative protein of unknown function Gene:AIM22(YJL046W)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Putative lipoate-protein ligase, required along with Lip2 and Lip5 for lipoylation of Lat1p and Kgd2p; similar to E. coli LplA; null mutant displays reduced frequency of mitochondrial genome loss Gene:APE4(YHR113W)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Cytoplasmic aspartyl aminopeptidase that may also function in the vacuole; CVT pathway cargo protein; cleaves unblocked N-terminal acidic amino acids from peptide substrates; forms a 12-subunit homo-oligomer; M18 metalloprotease family Gene:ARO1(YDR127W)|FD-Score:6.13|P-value:4.31E-10||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO7(YPR060C)|FD-Score:4.84|P-value:6.36E-7||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:ATG32(YIL146C)|FD-Score:3.22|P-value:6.32E-4||SGD DESC:Mitochondrial outer membrane protein required to initiate mitophagy; recruits the autophagy adaptor protein Atg11p and the ubiquitin-like protein Atg8p to the mitochondrial surface to initiate mitophagy, the selective vacuolar degradation of mitochondria in response to starvation; can promote pexophagy when placed ectopically in the peroxisomal membrane Gene:CEM1(YER061C)|FD-Score:7.05|P-value:9.17E-13||SGD DESC:Mitochondrial beta-keto-acyl synthase with possible role in fatty acid synthesis; required for mitochondrial respiration Gene:CHS5(YLR330W)|FD-Score:5.75|P-value:4.34E-9||SGD DESC:Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane Gene:COX7(YMR256C)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Subunit VII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CYS3(YAL012W)|FD-Score:5.97|P-value:1.16E-9||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:CYS4(YGR155W)|FD-Score:6.09|P-value:5.82E-10||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:DAL81(YIR023W)|FD-Score:5.89|P-value:1.96E-9||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DFG16(YOR030W)|FD-Score:6.25|P-value:2.07E-10||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:DSE2(YHR143W)|FD-Score:4.75|P-value:1.01E-6||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:EAF3(YPR023C)|FD-Score:4.35|P-value:6.89E-6||SGD DESC:Esa1p-associated factor, nonessential component of the NuA4 acetyltransferase complex, homologous to Drosophila dosage compensation protein MSL3; plays a role in regulating Ty1 transposition Gene:ECM22(YLR228C)|FD-Score:3.29|P-value:4.97E-4||SGD DESC:Sterol regulatory element binding protein; regulates transcription of sterol biosynthetic genes; contains Zn[2]-Cys[6] binuclear cluster; relocates from intracellular membranes to perinuclear foci on sterol depletion; ECM22 has a paralog, UPC2, that arose from the whole genome duplication Gene:ECT1(YGR007W)|FD-Score:4.53|P-value:2.98E-6||SGD DESC:Ethanolamine-phosphate cytidylyltransferase, catalyzes the second step of phosphatidylethanolamine biosynthesis; involved in the maintenance of plasma membrane; similar to mammalian CTP: phosphocholine cytidylyl-transferases Gene:ERG2(YMR202W)|FD-Score:5.26|P-value:7.04E-8||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ETT1(YOR051C)|FD-Score:4.1|P-value:2.04E-5||SGD DESC:Nuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts; deletion increases stop codon readthrough Gene:FIS1(YIL065C)|FD-Score:3.34|P-value:4.18E-4||SGD DESC:Protein involved in mitochondrial membrane fission and peroxisome abundance; required for localization of Dnm1p and Mdv1p during mitochondrial division; mediates ethanol-induced apoptosis and ethanol-induced mitochondrial fragmentation Gene:FZO1(YBR179C)|FD-Score:3.75|P-value:8.74E-5||SGD DESC:Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system Gene:GCN4(YEL009C)|FD-Score:4.28|P-value:9.19E-6||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GEM1(YAL048C)|FD-Score:3.1|P-value:9.80E-4||SGD DESC:Outer mitochondrial membrane GTPase, subunit of the ERMES complex; potential regulatory subunit of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth; cells lacking Gem1p contain collapsed, globular, or grape-like mitochondria; ortholog of metazoan Miro GTPases Gene:GIS3(YLR094C)|FD-Score:3.42|P-value:3.11E-4||SGD DESC:Protein of unknown function Gene:GPB2(YAL056W)|FD-Score:3.23|P-value:6.16E-4||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; inhibits Ras activity through direct interactions with Ira1p/2p; regulated by G-alpha protein Gpa2p; GPB2 has a paralog, GPB1, that arose from the whole genome duplication Gene:GUD1(YDL238C)|FD-Score:-3.64|P-value:1.38E-4||SGD DESC:Guanine deaminase, a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures Gene:HMG1(YML075C)|FD-Score:8.66|P-value:2.45E-18||SGD DESC:HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress Gene:HMG2(YLR450W)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:HMG-CoA reductase; converts HMG-CoA to mevalonate, a rate-limiting step in sterol biosynthesis; one of two isozymes; overproduction induces assembly of peripheral ER membrane arrays and short nuclear-associated membrane stacks; forms foci at the nuclear periphery upon DNA replication stress Gene:HOS2(YGL194C)|FD-Score:8.07|P-value:3.44E-16||SGD DESC:Histone deacetylase and subunit of Set3 and Rpd3L complexes; required for gene activation via specific deacetylation of lysines in H3 and H4 histone tails; subunit of the Set3 complex, a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; co-localizes with Cmr1p in nuclear foci in response to DNA damage by MMS Gene:HTD2(YHR067W)|FD-Score:3.78|P-value:7.71E-5||SGD DESC:Mitochondrial 3-hydroxyacyl-thioester dehydratase involved in fatty acid biosynthesis, required for respiratory growth and for normal mitochondrial morphology Gene:IMP2'(YIL154C)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:IRE1(YHR079C)|FD-Score:3.12|P-value:9.17E-4||SGD DESC:Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress Gene:LCL1(YPL056C_p)|FD-Score:-3.09|P-value:0.00100||SGD DESC:Putative protein of unknown function; deletion mutant is fluconazole resistant and has long chronological lifespan Gene:LIP2(YLR239C)|FD-Score:3.96|P-value:3.72E-5||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:LIP5(YOR196C)|FD-Score:5.42|P-value:3.05E-8||SGD DESC:Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthase Gene:LPD1(YFL018C)|FD-Score:5.37|P-value:4.05E-8||SGD DESC:Dihydrolipoamide dehydrogenase, the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multi-enzyme complexes Gene:MIP1(YOR330C)|FD-Score:3.54|P-value:1.96E-4||SGD DESC:Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit Gene:MRPL13(YKR006C)|FD-Score:3.24|P-value:6.01E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MRPL33(YMR286W)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRS3(YJL133W)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Iron transporter, mediates Fe2+ transport across inner mito membrane; mitochondrial carrier family member; active under low-iron conditions; may transport other cations; MRS3 has a paralog, MRS4, that arose from the whole genome duplication Gene:MRS4(YKR052C)|FD-Score:5.5|P-value:1.92E-8||SGD DESC:Iron transporter of the mitochondrial carrier family; mediates Fe2+ transport across the inner mitochondrial membrane; active under low-iron conditions; may transport other cations; protein abundance increases in response to DNA replication stress; MRS4 has a paralog, MRS3, that arose from the whole genome duplication Gene:MST1(YKL194C)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:MTM1(YGR257C)|FD-Score:6.95|P-value:1.81E-12||SGD DESC:Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor Gene:NFU1(YKL040C)|FD-Score:3.1|P-value:9.75E-4||SGD DESC:Protein involved in iron metabolism in mitochondria; similar to NifU, which is a protein required for the maturation of the Fe/S clusters of nitrogenase in nitrogen-fixing bacteria Gene:OAR1(YKL055C)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a type II mitochondrial fatty acid synthase along with Mct1p Gene:PDB1(YBR221C)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved multi-protein complex found in mitochondria Gene:PET123(YOR158W)|FD-Score:4.65|P-value:1.65E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator Gene:PGD1(YGL025C)|FD-Score:-3.39|P-value:3.44E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PIN4(YBL051C)|FD-Score:4.48|P-value:3.69E-6||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:RIM101(YHL027W)|FD-Score:8.21|P-value:1.08E-16||SGD DESC:Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC Gene:RIM13(YMR154C)|FD-Score:6.72|P-value:9.38E-12||SGD DESC:Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB Gene:RIM20(YOR275C)|FD-Score:5.04|P-value:2.32E-7||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation Gene:RIM21(YNL294C)|FD-Score:4.38|P-value:5.80E-6||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:RIM8(YGL045W)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family Gene:RML2(YEL050C)|FD-Score:3.19|P-value:7.02E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:SAC6(YDR129C)|FD-Score:-3.13|P-value:8.77E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SAS2(YMR127C)|FD-Score:4.11|P-value:1.97E-5||SGD DESC:Histone acetyltransferase (HAT) catalytic subunit of the SAS complex (Sas2p-Sas4p-Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; member of the MYSTacetyltransferase family Gene:SET3(YKR029C)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Defining member of the SET3 histone deacetylase complex which is a meiosis-specific repressor of sporulation genes; necessary for efficient transcription by RNAPII; one of two yeast proteins that contains both SET and PHD domains; SET3 has a paralog, SET4, that arose from the whole genome duplication Gene:SGE1(YPR198W)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SLA1(YBL007C)|FD-Score:4.88|P-value:5.30E-7||SGD DESC:Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains Gene:SNT1(YCR033W)|FD-Score:6.77|P-value:6.27E-12||SGD DESC:Subunit of the Set3C deacetylase complex that interacts directly with the Set3C subunit, Sif2p; putative DNA-binding protein; mutant has increased aneuploidy tolerance Gene:SPF1(YEL031W)|FD-Score:8.16|P-value:1.63E-16||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SPT3(YDR392W)|FD-Score:-3.2|P-value:6.79E-4||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SRB5(YGR104C)|FD-Score:3.75|P-value:8.98E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance Gene:THI2(YBR240C)|FD-Score:3.18|P-value:7.35E-4||SGD DESC:Transcriptional activator of thiamine biosynthetic genes; interacts with regulatory factor Thi3p to control expression of thiamine biosynthetic genes with respect to thiamine availability; acts together with Pdc2p to respond to thiaminediphosphate demand, possibly as related to carbon source availability; zinc finger protein of the Zn(II)2Cys6 type Gene:THR4(YCR053W)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TOM5(YPR133W-A)|FD-Score:4.6|P-value:2.09E-6||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import of all mitochondrially directed proteins; involved in transfer of precursors from the Tom70p and Tom20p receptors to the Tom40p pore Gene:TRP3(YKL211C)|FD-Score:3.31|P-value:4.69E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UTR1(YJR049C)|FD-Score:6.44|P-value:5.91E-11||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:YAP3(YHL009C)|FD-Score:4.11|P-value:1.99E-5||SGD DESC:Basic leucine zipper (bZIP) transcription factor Gene:YBL029C-A(YBL029C-A_p)|FD-Score:4.79|P-value:8.40E-7||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress; has potential orthologs in Saccharomyces species and in Yarrowia lipolytica Gene:YCL046W(YCL046W_d)|FD-Score:5.92|P-value:1.62E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C Gene:YDL023C(YDL023C_d)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in other Saccharomyces species; overlaps the verified gene GPD1; deletion confers sensitivity to GSAO; deletion in cyr1 mutant results in loss of stress resistance Gene:YGR122W(YGR122W)|FD-Score:6.54|P-value:3.17E-11||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YIA6(YIL006W)|FD-Score:3.68|P-value:1.19E-4||SGD DESC:Mitochondrial NAD+ transporter, involved in the transport of NAD+ into the mitochondria (see also YEA6); member of the mitochondrial carrier subfamily; disputed role as a pyruvate transporter; has putative mouse and human orthologs Gene:YLR118C(YLR118C)|FD-Score:4.01|P-value:2.99E-5||SGD DESC:Acyl-protein thioesterase responsible for depalmitoylation of Gpa1p; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS Gene:YML007C-A(YML007C-A_p)|FD-Score:4.45|P-value:4.31E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YNL042W-B(YNL042W-B_p)|FD-Score:3.22|P-value:6.52E-4||SGD DESC:Putative protein of unknown function

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOL134C_d4.299.12E-60.69YOL134C_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps HRT1, a verified gene that encodes an SCF ubiquitin ligase subunit
YLR153C4.022.86E-50.69ACS2Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions
YIR010W3.334.35E-40.09DSN1Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; important for chromosome segregation
YKR004C3.245.95E-40.02ECM9Non-essential protein of unknown function
YHR072W3.226.40E-40.24ERG7Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis
YLR359W2.980.001450.07ADE13Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency
YIL026C2.910.001820.03IRR1Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability
YDR390C2.880.001980.01UBA2Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Aos1p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability
YDL207W2.870.002030.14GLE1Cytoplasmic nucleoporin required for polyadenylated RNA export; not essential for protein import; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export
YNR011C2.730.003170.09PRP2RNA-dependent ATPase in the DEAH-box family, required for activation of the spliceosome before the first transesterification step in RNA splicing; orthologous to human protein DHX16
YLR259C2.640.004160.07HSP60Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides and complex assembly; prevents aggregation and mediates protein refolding after heat shock; role in mtDNA transmission; phosphorylated
YPL028W2.570.005150.04ERG10Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase), cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis
YHR023W2.520.005850.06MYO1Type II myosin heavy chain, required for wild-type cytokinesis and cell separation; localizes to the actomyosin ring; binds to myosin light chains Mlc1p and Mlc2p through its IQ1 and IQ2 motifs respectively
YLR222C2.460.007020.03UTP13Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YJR022W2.430.007610.03LSM8Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) that is part of spliceosomal U6 snRNP and is also implicated in processing of pre-tRNA, pre-snoRNA, and pre-rRNA

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YML075C8.662.45E-18HMG1HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress
YHL027W8.211.08E-16RIM101Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression as part of adaptation to a alkaline conditions; involved in cell wall assembly; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC
YEL031W8.161.63E-16SPF1P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1
YGL194C8.073.44E-16HOS2Histone deacetylase and subunit of Set3 and Rpd3L complexes; required for gene activation via specific deacetylation of lysines in H3 and H4 histone tails; subunit of the Set3 complex, a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; co-localizes with Cmr1p in nuclear foci in response to DNA damage by MMS
YER061C7.059.17E-13CEM1Mitochondrial beta-keto-acyl synthase with possible role in fatty acid synthesis; required for mitochondrial respiration
YGR257C6.951.81E-12MTM1Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor
YCR033W6.776.27E-12SNT1Subunit of the Set3C deacetylase complex that interacts directly with the Set3C subunit, Sif2p; putative DNA-binding protein; mutant has increased aneuploidy tolerance
YMR154C6.729.38E-12RIM13Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB
YGR122W6.543.17E-11YGR122WProtein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress
YJR049C6.445.91E-11UTR1ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication
YOR030W6.252.07E-10DFG16Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p
YDR127W6.134.31E-10ARO1Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
YGR155W6.095.82E-10CYS4Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria
YAL012W5.971.16E-9CYS3Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress
YCL046W_d5.921.62E-9YCL046W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C

GO enrichment analysis for SGTC_279
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.4071.84E-234SGTC_13171273-0028 910.0 nMChemDiv (Drug-like library)59178030.326087azole & statin
0.3291.80E-148SGTC_3271273-0057 4.0 μMChemDiv (Drug-like library)31081380.575azole & statin
0.3235.56E-143SGTC_8620452-0056 10.2 μMChemDiv (Drug-like library)15753960.32azole & statin
0.2392.31E-77SGTC_1788fluconazole 20.0 μMMiscellaneous33650.047619azole & statin
0.2294.85E-71SGTC_9021120-0002 2.1 μMChemDiv (Drug-like library)32804750.0731707azole & statin
0.2171.04E-63SGTC_2648atorvastatin 78.7 μMMiscellaneous608230.045977azole & statin
0.2131.29E-61SGTC_227fluconazole 33.5 μMMiscellaneous33650.047619azole & statin
0.1967.18E-52SGTC_8520418-0143 439.0 μMChemDiv (Drug-like library)53692290.0172414calcium & mitochondrial duress
0.1938.45E-51SGTC_1084voriconazole 435.0 nMNIH Clinical Collection716160.0526316azole & statin
0.1932.35E-50SGTC_2647atorvastatin 60.2 μMMiscellaneous608230.045977azole & statin
0.1872.38E-47SGTC_10194262-0212 3.4 μMChemDiv (Drug-like library)46772130.030303ergosterol depletion effects on membrane
0.1722.00E-40SGTC_1656st012921 11.0 μMTimTec (Natural product derivative library)67328210.0307692
0.1714.57E-40SGTC_2523spermine 47.7 μMMicrosource (Natural product library)11030.047619calcium & mitochondrial duress
0.1696.87E-39SGTC_2765myclobutanil 7.7 μMMiscellaneous63360.177419azole & statin
0.1611.50E-35SGTC_29259010536 32.5 μMChembridge (Drug-like library)29941770.0819672azole & statin

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3271273-00573.96 μM0.5753108138ChemDiv (Drug-like library)349.573747.6501azole & statin
SGTC_3101273-00581.06 μM0.5555562734232ChemDiv (Drug-like library)307.537068.06800mitochondrial stress
SGTC_1791273-00636.77 μM0.5227273108139ChemDiv (Drug-like library)366.56126.93522
SGTC_1931273-00601.56 μM0.4651162847393ChemDiv (Drug-like library)369.606449.64500ergosterol biosynthesis
SGTC_13171273-0028910 nM0.3260875917803ChemDiv (Drug-like library)347.557866.43612azole & statin
SGTC_8620452-005610.2 μM0.321575396ChemDiv (Drug-like library)303.465546.22302azole & statin
SGTC_2991273-00591.44 μM0.2916672848077ChemDiv (Drug-like library)357.595749.5600
SGTC_1731273-00612.69 μM0.274512847414ChemDiv (Drug-like library)419.6651211.13600mitochondrial stress
SGTC_12470448-00435.84 μM0.2452833089013ChemDiv (Drug-like library)370.379547.99200ERG2
SGTC_1843st05580945.5 μM0.20967716413835TimTec (Natural product derivative library)437.314532.60903