7325131

N-(4-ethylphenyl)-2-(4-fluorophenoxy)propanamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2793
Screen concentration 81.8 μM
Source Chembridge (Drug-like library)
PubChem CID 4176441
SMILES CCC1=CC=C(C=C1)NC(=O)C(C)OC2=CC=C(C=C2)F
Standardized SMILES CCc1ccc(NC(=O)C(C)Oc2ccc(F)cc2)cc1
Molecular weight 287.3287
ALogP 4.08
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 7.86
% growth inhibition (Hom. pool) 4.86


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4176441
Download HIP data (tab-delimited text)  (excel)
Gene:ARP7(YPR034W)|FD-Score:3.92|P-value:4.44E-5|Clearance:0.19||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CDC25(YLR310C)|FD-Score:3.73|P-value:9.57E-5|Clearance:0.07||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:DNA2(YHR164C)|FD-Score:4.02|P-value:2.92E-5|Clearance:0.1||SGD DESC:Tripartite DNA replication factor; has single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; required for Okazaki fragment processing; involved in DNA repair; cell-cycle dependent localization; forms nuclear foci upon DNA replication stress Gene:GCD10(YNL062C)|FD-Score:3.37|P-value:3.80E-4|Clearance:0.12||SGD DESC:Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression Gene:MRPS18(YNL306W)|FD-Score:3.61|P-value:1.51E-4|Clearance:0.25||SGD DESC:Mitochondrial ribosomal protein of the small subunit; essential for viability, unlike most other mitoribosomal proteins Gene:NIC96(YFR002W)|FD-Score:4.51|P-value:3.31E-6|Clearance:0.49||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:PGA3(YML125C)|FD-Score:-3.38|P-value:3.60E-4|Clearance:0||SGD DESC:Putative cytochrome b5 reductase, localized to the plasma membrane; may be involved in regulation of lifespan; required for maturation of Gas1p and Pho8p, proposed to be involved in protein trafficking; PGA3 has a paralog, AIM33, that arose from the whole genome duplication Gene:PHS1(YJL097W)|FD-Score:4.75|P-value:1.01E-6|Clearance:0.07||SGD DESC:Essential 3-hydroxyacyl-CoA dehydratase of the ER membrane, involved in elongation of very long-chain fatty acids; evolutionarily conserved, similar to mammalian PTPLA and PTPLB; involved in sphingolipid biosynthesis and protein trafficking Gene:PKC1(YBL105C)|FD-Score:-3.86|P-value:5.78E-5|Clearance:0||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RPL15A(YLR029C)|FD-Score:-3.33|P-value:4.33E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:RPP1(YHR062C)|FD-Score:4.68|P-value:1.45E-6|Clearance:0.17||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RSC58(YLR033W)|FD-Score:-3.41|P-value:3.25E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SCL1(YGL011C)|FD-Score:3.18|P-value:7.41E-4|Clearance:0.02||SGD DESC:Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria Gene:SDO1(YLR022C)|FD-Score:-3.34|P-value:4.24E-4|Clearance:0||SGD DESC:Essential protein involved in ribosome maturation; with Ria1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; ortholog of the human protein (SBDS) responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome; highly conserved across archaea and eukaryotes Gene:SEC6(YIL068C)|FD-Score:5.67|P-value:7.02E-9|Clearance:0.75||SGD DESC:Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p Gene:SNM1(YDR478W)|FD-Score:3.16|P-value:7.97E-4|Clearance:0.09||SGD DESC:Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP Gene:SWD2(YKL018W)|FD-Score:-5.82|P-value:2.89E-9|Clearance:0||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination Gene:TIF6(YPR016C)|FD-Score:3.66|P-value:1.27E-4|Clearance:0.04||SGD DESC:Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits Gene:UFD1(YGR048W)|FD-Score:5.51|P-value:1.84E-8|Clearance:0.75||SGD DESC:Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transaction factors by regulated Ub/proteasome-dependent processing (RUP) Gene:USE1(YGL098W)|FD-Score:-3.35|P-value:4.07E-4|Clearance:0||SGD DESC:Essential SNARE protein localized to the ER, involved in retrograde traffic from the Golgi to the ER; forms a complex with the SNAREs Sec22p, Sec20p and Ufe1p Gene:UTP8(YGR128C)|FD-Score:3.25|P-value:5.83E-4|Clearance:0.07||SGD DESC:Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YDL152W(YDL152W_d)|FD-Score:3.66|P-value:1.24E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:ARP7(YPR034W)|FD-Score:3.92|P-value:4.44E-5|Clearance:0.19||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CDC25(YLR310C)|FD-Score:3.73|P-value:9.57E-5|Clearance:0.07||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:DNA2(YHR164C)|FD-Score:4.02|P-value:2.92E-5|Clearance:0.1||SGD DESC:Tripartite DNA replication factor; has single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; required for Okazaki fragment processing; involved in DNA repair; cell-cycle dependent localization; forms nuclear foci upon DNA replication stress Gene:GCD10(YNL062C)|FD-Score:3.37|P-value:3.80E-4|Clearance:0.12||SGD DESC:Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression Gene:MRPS18(YNL306W)|FD-Score:3.61|P-value:1.51E-4|Clearance:0.25||SGD DESC:Mitochondrial ribosomal protein of the small subunit; essential for viability, unlike most other mitoribosomal proteins Gene:NIC96(YFR002W)|FD-Score:4.51|P-value:3.31E-6|Clearance:0.49||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:PGA3(YML125C)|FD-Score:-3.38|P-value:3.60E-4|Clearance:0||SGD DESC:Putative cytochrome b5 reductase, localized to the plasma membrane; may be involved in regulation of lifespan; required for maturation of Gas1p and Pho8p, proposed to be involved in protein trafficking; PGA3 has a paralog, AIM33, that arose from the whole genome duplication Gene:PHS1(YJL097W)|FD-Score:4.75|P-value:1.01E-6|Clearance:0.07||SGD DESC:Essential 3-hydroxyacyl-CoA dehydratase of the ER membrane, involved in elongation of very long-chain fatty acids; evolutionarily conserved, similar to mammalian PTPLA and PTPLB; involved in sphingolipid biosynthesis and protein trafficking Gene:PKC1(YBL105C)|FD-Score:-3.86|P-value:5.78E-5|Clearance:0||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RPL15A(YLR029C)|FD-Score:-3.33|P-value:4.33E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:RPP1(YHR062C)|FD-Score:4.68|P-value:1.45E-6|Clearance:0.17||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RSC58(YLR033W)|FD-Score:-3.41|P-value:3.25E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SCL1(YGL011C)|FD-Score:3.18|P-value:7.41E-4|Clearance:0.02||SGD DESC:Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria Gene:SDO1(YLR022C)|FD-Score:-3.34|P-value:4.24E-4|Clearance:0||SGD DESC:Essential protein involved in ribosome maturation; with Ria1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; ortholog of the human protein (SBDS) responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome; highly conserved across archaea and eukaryotes Gene:SEC6(YIL068C)|FD-Score:5.67|P-value:7.02E-9|Clearance:0.75||SGD DESC:Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p Gene:SNM1(YDR478W)|FD-Score:3.16|P-value:7.97E-4|Clearance:0.09||SGD DESC:Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP Gene:SWD2(YKL018W)|FD-Score:-5.82|P-value:2.89E-9|Clearance:0||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination Gene:TIF6(YPR016C)|FD-Score:3.66|P-value:1.27E-4|Clearance:0.04||SGD DESC:Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits Gene:UFD1(YGR048W)|FD-Score:5.51|P-value:1.84E-8|Clearance:0.75||SGD DESC:Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transaction factors by regulated Ub/proteasome-dependent processing (RUP) Gene:USE1(YGL098W)|FD-Score:-3.35|P-value:4.07E-4|Clearance:0||SGD DESC:Essential SNARE protein localized to the ER, involved in retrograde traffic from the Golgi to the ER; forms a complex with the SNAREs Sec22p, Sec20p and Ufe1p Gene:UTP8(YGR128C)|FD-Score:3.25|P-value:5.83E-4|Clearance:0.07||SGD DESC:Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YDL152W(YDL152W_d)|FD-Score:3.66|P-value:1.24E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4176441
Download HOP data (tab-delimited text)  (excel)
Gene:ASR1(YPR093C)|FD-Score:3.27|P-value:5.39E-4||SGD DESC:Ubiquitin ligase that modifies and regulates RNA Pol II; involved in a putative alcohol-responsive signaling pathway; accumulates in the nucleus under alcohol stress; contains a Ring/PHD finger domain similar to the mammalian rA9 protein Gene:ATG10(YLL042C)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:Conserved E2-like conjugating enzyme that mediates formation of the Atg12p-Atg5p conjugate, which is a critical step in autophagy Gene:BER1(YLR412W)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Protein involved in microtubule-related processes, N-acetylation; GFP-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YLR412W is not an essential gene; similar to Arabidopsis SRR1 gene Gene:BNR1(YIL159W)|FD-Score:5.39|P-value:3.45E-8||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNI1 Gene:BUD26(YDR241W_d)|FD-Score:-3.57|P-value:1.78E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 1% of ORF overlaps the verified gene SNU56; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CIN4(YMR138W)|FD-Score:-3.8|P-value:7.09E-5||SGD DESC:GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog Gene:COX10(YPL172C)|FD-Score:-4.13|P-value:1.82E-5||SGD DESC:Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders Gene:CTK3(YML112W)|FD-Score:-4.81|P-value:7.47E-7||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:DAL1(YIR027C)|FD-Score:3.14|P-value:8.53E-4||SGD DESC:Allantoinase, converts allantoin to allantoate in the first step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:DAS1(YJL149W)|FD-Score:6.27|P-value:1.79E-10||SGD DESC:Putative SCF ubiquitin ligase F-box protein; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C Gene:DGR2(YKL121W_p)|FD-Score:3.34|P-value:4.14E-4||SGD DESC:Protein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds Gene:ENT3(YJR125C)|FD-Score:3.48|P-value:2.46E-4||SGD DESC:Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p Gene:ESC1(YMR219W)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Protein localized to the nuclear periphery, involved in telomeric silencing; interacts with PAD4-domain of Sir4p Gene:FCJ1(YKR016W)|FD-Score:-3.2|P-value:6.97E-4||SGD DESC:Mitochondrial inner membrane protein, ortholog of mammalian mitofilin; involved in import of intermembrane space (IMS) proteins, probably by positioning Mia40p relative to the TOM complex to receive incoming proteins; also has an essential role in the maintenance of crista junctions and inner membrane architecture, as a component of the mitochondrial inner membrane organizing system (MICOS, MitOS or MINOS), a scaffold-like structure on the IMS side of the inner membrane Gene:GET2(YER083C)|FD-Score:-4.05|P-value:2.51E-5||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:HIT1(YJR055W)|FD-Score:-3.3|P-value:4.82E-4||SGD DESC:Protein of unknown function, required for growth at high temperature Gene:IDP1(YDL066W)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes Gene:IRC3(YDR332W)|FD-Score:-4.41|P-value:5.13E-6||SGD DESC:Putative RNA helicase of the DEAH/D-box family; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:LYS14(YDR034C)|FD-Score:3.12|P-value:9.06E-4||SGD DESC:Transcriptional activator involved in regulation of genes of the lysine biosynthesis pathway; requires 2-aminoadipate semialdehyde as co-inducer Gene:MEF2(YJL102W)|FD-Score:-4.49|P-value:3.58E-6||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MOH1(YBL049W)|FD-Score:-3.23|P-value:6.14E-4||SGD DESC:Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase Gene:MRK1(YDL079C)|FD-Score:3.17|P-value:7.52E-4||SGD DESC:Glycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation Gene:MRPL9(YGR220C)|FD-Score:-3.39|P-value:3.55E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSF1(YPR047W)|FD-Score:4.13|P-value:1.80E-5||SGD DESC:Mitochondrial phenylalanyl-tRNA synthetase, active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase Gene:OPY2(YPR075C)|FD-Score:4.07|P-value:2.37E-5||SGD DESC:Integral membrane protein that acts as a membrane anchor for Ste50p; involved in the signaling branch of the high-osmolarity glycerol (HOG) pathway and as a regulator of the filamentous growth pathway; overproduction blocks cell cycle arrest in the presence of mating pheromone; relocalizes from vacuole to plasma membrane upon DNA replication stress Gene:OSM1(YJR051W)|FD-Score:4.96|P-value:3.59E-7||SGD DESC:Fumarate reductase, catalyzes the reduction of fumarate to succinate; required for the reoxidation of intracellular NADH under anaerobic conditions; mutations cause osmotic sensitivity; OSM1 has a paralog, FRD1, that arose from the whole genome duplication Gene:PEX12(YMR026C)|FD-Score:3.15|P-value:8.20E-4||SGD DESC:C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder Gene:PEX13(YLR191W)|FD-Score:-3.42|P-value:3.13E-4||SGD DESC:Integral peroxisomal membrane protein required for translocation of peroxisomal matrix proteins, interacts with the PTS1 signal recognition factor Pex5p and the PTS2 signal recognition factor Pex7p, forms a complex with Pex14p and Pex17p Gene:PGD1(YGL025C)|FD-Score:3.9|P-value:4.83E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:RAM1(YDL090C)|FD-Score:-3.46|P-value:2.74E-4||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RAV2(YDR202C)|FD-Score:-3.36|P-value:3.87E-4||SGD DESC:Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme Gene:REC8(YPR007C)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Meiosis-specific component of sister chromatid cohesion complex; maintains cohesion between sister chromatids during meiosis I; maintains cohesion between centromeres of sister chromatids until meiosis II; homolog of S. pombe Rec8p Gene:RHO5(YNL180C)|FD-Score:4.52|P-value:3.15E-6||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely involved in protein kinase C (Pkc1p)-dependent signal transduction pathway that controls cell integrity Gene:RPA14(YDR156W)|FD-Score:-3.42|P-value:3.17E-4||SGD DESC:RNA polymerase I subunit A14 Gene:RPL43A(YPR043W)|FD-Score:6.46|P-value:5.38E-11||SGD DESC:Ribosomal 60S subunit protein L43A; null mutation confers a dominant lethal phenotype; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43A has a paralog, RPL43B, that arose from the whole genome duplication Gene:RPL9A(YGL147C)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Ribosomal 60S subunit protein L9A; nearly identical to paralog Rpl9Bp; homologous to mammalian ribosomal protein L9 and bacterial L6 Gene:RPS6A(YPL090C)|FD-Score:3.31|P-value:4.62E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6A has a paralog, RPS6B, that arose from the whole genome duplication Gene:RTC1(YOL138C)|FD-Score:6.6|P-value:2.09E-11||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; null mutation suppresses cdc13-1 temperature sensitivity; has N-terminal WD-40 repeats and a C-terminal RING motif Gene:RTT101(YJL047C)|FD-Score:6.72|P-value:9.06E-12||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:SBE2(YDR351W)|FD-Score:-3.53|P-value:2.04E-4||SGD DESC:Protein involved in the transport of cell wall components from the Golgi to the cell surface; required for bud growth Gene:SEC72(YLR292C)|FD-Score:3.91|P-value:4.58E-5||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SET3(YKR029C)|FD-Score:-4.83|P-value:6.84E-7||SGD DESC:Defining member of the SET3 histone deacetylase complex which is a meiosis-specific repressor of sporulation genes; necessary for efficient transcription by RNAPII; one of two yeast proteins that contains both SET and PHD domains; SET3 has a paralog, SET4, that arose from the whole genome duplication Gene:SIS2(YKR072C)|FD-Score:-3.13|P-value:8.69E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SKI3(YPR189W)|FD-Score:-6.11|P-value:5.02E-10||SGD DESC:Ski complex component and TPR protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SMA1(YPL027W)|FD-Score:3.79|P-value:7.62E-5||SGD DESC:Protein of unknown function involved in the assembly of the prospore membrane during sporulation; interacts with Spo14p Gene:SNZ2(YNL333W)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO2; interacts with Thi11p Gene:SOK1(YDR006C)|FD-Score:-3.75|P-value:8.84E-5||SGD DESC:Protein whose overexpression suppresses the growth defect of mutants lacking protein kinase A activity; involved in cAMP-mediated signaling; localized to the nucleus; similar to the mouse testis-specific protein PBS13 Gene:SPO11(YHL022C)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Meiosis-specific protein that initiates meiotic recombination by catalyzing the formation of double-strand breaks in DNA via a transesterification reaction; required for homologous chromosome pairing and synaptonemal complex formation Gene:SUC2(YIL162W)|FD-Score:-3.34|P-value:4.21E-4||SGD DESC:Invertase, sucrose hydrolyzing enzyme; a secreted, glycosylated form is regulated by glucose repression, and an intracellular, nonglycosylated enzyme is produced constitutively Gene:SUR2(YDR297W)|FD-Score:7.41|P-value:6.26E-14||SGD DESC:Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis Gene:SVS1(YPL163C)|FD-Score:-3.86|P-value:5.57E-5||SGD DESC:Cell wall and vacuolar protein; required for wild-type resistance to vanadate; SVS1 has a paralog, SRL1, that arose from the whole genome duplication Gene:SWC5(YBR231C)|FD-Score:4.24|P-value:1.11E-5||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:SWI5(YDR146C)|FD-Score:-4.04|P-value:2.66E-5||SGD DESC:Transcription factor that recruits Mediator and Swi/Snf complexes; activates transcription of genes expressed at the M/G1 phase boundary and in G1 phase; required for expression of the HO gene controlling mating type switching; localization to nucleus occurs during G1 and appears to be regulated by phosphorylation by Cdc28p kinase; SWI5 has a paralog, ACE2, that arose from the whole genome duplication Gene:SYP1(YCR030C)|FD-Score:-4.6|P-value:2.14E-6||SGD DESC:Protein of unknown function that is involved in endocytic site formation; may regulate assembly and disassembly of the septin ring; colocalizes and interacts with septin subunits; potential role in actin cytoskeletal organization Gene:TSC3(YBR058C-A)|FD-Score:3.48|P-value:2.46E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VMA1(YDL185W)|FD-Score:3.15|P-value:8.24E-4||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VPS21(YOR089C)|FD-Score:-6.77|P-value:6.48E-12||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS25(YJR102C)|FD-Score:-3.37|P-value:3.78E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:YBL100W-C(YBL100W-C_p)|FD-Score:4.59|P-value:2.23E-6||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YDR290W(YDR290W_d)|FD-Score:-4.25|P-value:1.07E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Gene:YER175W-A(YER175W-A_p)|FD-Score:-3.41|P-value:3.26E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YER184C(YER184C_p)|FD-Score:3.83|P-value:6.45E-5||SGD DESC:Putative zinc cluster protein; deletion confers sensitivity to Calcufluor white, and prevents growth on glycerol or lactate as sole carbon source Gene:YFR006W(YFR006W_p)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Putative X-Pro aminopeptidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YFR006W is not an essential gene Gene:YGL007C-A(YGL007C-A_p)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Putative protein of unknown function, identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; deletion exhibits slow-growth phenotype; computationally predicted to have a role in cell budding Gene:YGR015C(YGR015C_p)|FD-Score:4.56|P-value:2.61E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:YGR127W(YGR127W_p)|FD-Score:5.53|P-value:1.65E-8||SGD DESC:Putative protein of unknown function; expression is regulated by Msn2p/Msn4p, indicating a possible role in stress response Gene:YIL029C(YIL029C_p)|FD-Score:-3.41|P-value:3.28E-4||SGD DESC:Putative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YIL029C has paralog YPR071W Gene:YLR012C(YLR012C_p)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Putative protein of unknown function; YLR012C is not an essential gene Gene:YMR075C-A(YMR075C-A_d)|FD-Score:3.33|P-value:4.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RCO1/YMR075W Gene:YMR175W-A(YMR175W-A_p)|FD-Score:-3.25|P-value:5.75E-4||SGD DESC:Putative protein of unknown function Gene:YNL140C(YNL140C_p)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps THO2/YNL139C Gene:YOL013W-A(YOL013W-A_p)|FD-Score:3.17|P-value:7.55E-4||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YPL014W(YPL014W_p)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus Gene:YRB30(YGL164C)|FD-Score:-4.1|P-value:2.03E-5||SGD DESC:RanGTP-binding protein, inhibits RanGAP1 (Rna1p)-mediated GTP hydrolysis of RanGTP (Gsp1p); shares similarity to proteins in other fungi but not in higher eukaryotes Gene:ASR1(YPR093C)|FD-Score:3.27|P-value:5.39E-4||SGD DESC:Ubiquitin ligase that modifies and regulates RNA Pol II; involved in a putative alcohol-responsive signaling pathway; accumulates in the nucleus under alcohol stress; contains a Ring/PHD finger domain similar to the mammalian rA9 protein Gene:ATG10(YLL042C)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:Conserved E2-like conjugating enzyme that mediates formation of the Atg12p-Atg5p conjugate, which is a critical step in autophagy Gene:BER1(YLR412W)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Protein involved in microtubule-related processes, N-acetylation; GFP-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YLR412W is not an essential gene; similar to Arabidopsis SRR1 gene Gene:BNR1(YIL159W)|FD-Score:5.39|P-value:3.45E-8||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNI1 Gene:BUD26(YDR241W_d)|FD-Score:-3.57|P-value:1.78E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 1% of ORF overlaps the verified gene SNU56; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CIN4(YMR138W)|FD-Score:-3.8|P-value:7.09E-5||SGD DESC:GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog Gene:COX10(YPL172C)|FD-Score:-4.13|P-value:1.82E-5||SGD DESC:Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders Gene:CTK3(YML112W)|FD-Score:-4.81|P-value:7.47E-7||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:DAL1(YIR027C)|FD-Score:3.14|P-value:8.53E-4||SGD DESC:Allantoinase, converts allantoin to allantoate in the first step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:DAS1(YJL149W)|FD-Score:6.27|P-value:1.79E-10||SGD DESC:Putative SCF ubiquitin ligase F-box protein; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C Gene:DGR2(YKL121W_p)|FD-Score:3.34|P-value:4.14E-4||SGD DESC:Protein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds Gene:ENT3(YJR125C)|FD-Score:3.48|P-value:2.46E-4||SGD DESC:Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p Gene:ESC1(YMR219W)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Protein localized to the nuclear periphery, involved in telomeric silencing; interacts with PAD4-domain of Sir4p Gene:FCJ1(YKR016W)|FD-Score:-3.2|P-value:6.97E-4||SGD DESC:Mitochondrial inner membrane protein, ortholog of mammalian mitofilin; involved in import of intermembrane space (IMS) proteins, probably by positioning Mia40p relative to the TOM complex to receive incoming proteins; also has an essential role in the maintenance of crista junctions and inner membrane architecture, as a component of the mitochondrial inner membrane organizing system (MICOS, MitOS or MINOS), a scaffold-like structure on the IMS side of the inner membrane Gene:GET2(YER083C)|FD-Score:-4.05|P-value:2.51E-5||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:HIT1(YJR055W)|FD-Score:-3.3|P-value:4.82E-4||SGD DESC:Protein of unknown function, required for growth at high temperature Gene:IDP1(YDL066W)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes Gene:IRC3(YDR332W)|FD-Score:-4.41|P-value:5.13E-6||SGD DESC:Putative RNA helicase of the DEAH/D-box family; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:LYS14(YDR034C)|FD-Score:3.12|P-value:9.06E-4||SGD DESC:Transcriptional activator involved in regulation of genes of the lysine biosynthesis pathway; requires 2-aminoadipate semialdehyde as co-inducer Gene:MEF2(YJL102W)|FD-Score:-4.49|P-value:3.58E-6||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MOH1(YBL049W)|FD-Score:-3.23|P-value:6.14E-4||SGD DESC:Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase Gene:MRK1(YDL079C)|FD-Score:3.17|P-value:7.52E-4||SGD DESC:Glycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation Gene:MRPL9(YGR220C)|FD-Score:-3.39|P-value:3.55E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSF1(YPR047W)|FD-Score:4.13|P-value:1.80E-5||SGD DESC:Mitochondrial phenylalanyl-tRNA synthetase, active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase Gene:OPY2(YPR075C)|FD-Score:4.07|P-value:2.37E-5||SGD DESC:Integral membrane protein that acts as a membrane anchor for Ste50p; involved in the signaling branch of the high-osmolarity glycerol (HOG) pathway and as a regulator of the filamentous growth pathway; overproduction blocks cell cycle arrest in the presence of mating pheromone; relocalizes from vacuole to plasma membrane upon DNA replication stress Gene:OSM1(YJR051W)|FD-Score:4.96|P-value:3.59E-7||SGD DESC:Fumarate reductase, catalyzes the reduction of fumarate to succinate; required for the reoxidation of intracellular NADH under anaerobic conditions; mutations cause osmotic sensitivity; OSM1 has a paralog, FRD1, that arose from the whole genome duplication Gene:PEX12(YMR026C)|FD-Score:3.15|P-value:8.20E-4||SGD DESC:C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder Gene:PEX13(YLR191W)|FD-Score:-3.42|P-value:3.13E-4||SGD DESC:Integral peroxisomal membrane protein required for translocation of peroxisomal matrix proteins, interacts with the PTS1 signal recognition factor Pex5p and the PTS2 signal recognition factor Pex7p, forms a complex with Pex14p and Pex17p Gene:PGD1(YGL025C)|FD-Score:3.9|P-value:4.83E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:RAM1(YDL090C)|FD-Score:-3.46|P-value:2.74E-4||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RAV2(YDR202C)|FD-Score:-3.36|P-value:3.87E-4||SGD DESC:Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme Gene:REC8(YPR007C)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Meiosis-specific component of sister chromatid cohesion complex; maintains cohesion between sister chromatids during meiosis I; maintains cohesion between centromeres of sister chromatids until meiosis II; homolog of S. pombe Rec8p Gene:RHO5(YNL180C)|FD-Score:4.52|P-value:3.15E-6||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely involved in protein kinase C (Pkc1p)-dependent signal transduction pathway that controls cell integrity Gene:RPA14(YDR156W)|FD-Score:-3.42|P-value:3.17E-4||SGD DESC:RNA polymerase I subunit A14 Gene:RPL43A(YPR043W)|FD-Score:6.46|P-value:5.38E-11||SGD DESC:Ribosomal 60S subunit protein L43A; null mutation confers a dominant lethal phenotype; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43A has a paralog, RPL43B, that arose from the whole genome duplication Gene:RPL9A(YGL147C)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Ribosomal 60S subunit protein L9A; nearly identical to paralog Rpl9Bp; homologous to mammalian ribosomal protein L9 and bacterial L6 Gene:RPS6A(YPL090C)|FD-Score:3.31|P-value:4.62E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6A has a paralog, RPS6B, that arose from the whole genome duplication Gene:RTC1(YOL138C)|FD-Score:6.6|P-value:2.09E-11||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; null mutation suppresses cdc13-1 temperature sensitivity; has N-terminal WD-40 repeats and a C-terminal RING motif Gene:RTT101(YJL047C)|FD-Score:6.72|P-value:9.06E-12||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:SBE2(YDR351W)|FD-Score:-3.53|P-value:2.04E-4||SGD DESC:Protein involved in the transport of cell wall components from the Golgi to the cell surface; required for bud growth Gene:SEC72(YLR292C)|FD-Score:3.91|P-value:4.58E-5||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SET3(YKR029C)|FD-Score:-4.83|P-value:6.84E-7||SGD DESC:Defining member of the SET3 histone deacetylase complex which is a meiosis-specific repressor of sporulation genes; necessary for efficient transcription by RNAPII; one of two yeast proteins that contains both SET and PHD domains; SET3 has a paralog, SET4, that arose from the whole genome duplication Gene:SIS2(YKR072C)|FD-Score:-3.13|P-value:8.69E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SKI3(YPR189W)|FD-Score:-6.11|P-value:5.02E-10||SGD DESC:Ski complex component and TPR protein, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SMA1(YPL027W)|FD-Score:3.79|P-value:7.62E-5||SGD DESC:Protein of unknown function involved in the assembly of the prospore membrane during sporulation; interacts with Spo14p Gene:SNZ2(YNL333W)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO2; interacts with Thi11p Gene:SOK1(YDR006C)|FD-Score:-3.75|P-value:8.84E-5||SGD DESC:Protein whose overexpression suppresses the growth defect of mutants lacking protein kinase A activity; involved in cAMP-mediated signaling; localized to the nucleus; similar to the mouse testis-specific protein PBS13 Gene:SPO11(YHL022C)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Meiosis-specific protein that initiates meiotic recombination by catalyzing the formation of double-strand breaks in DNA via a transesterification reaction; required for homologous chromosome pairing and synaptonemal complex formation Gene:SUC2(YIL162W)|FD-Score:-3.34|P-value:4.21E-4||SGD DESC:Invertase, sucrose hydrolyzing enzyme; a secreted, glycosylated form is regulated by glucose repression, and an intracellular, nonglycosylated enzyme is produced constitutively Gene:SUR2(YDR297W)|FD-Score:7.41|P-value:6.26E-14||SGD DESC:Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis Gene:SVS1(YPL163C)|FD-Score:-3.86|P-value:5.57E-5||SGD DESC:Cell wall and vacuolar protein; required for wild-type resistance to vanadate; SVS1 has a paralog, SRL1, that arose from the whole genome duplication Gene:SWC5(YBR231C)|FD-Score:4.24|P-value:1.11E-5||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:SWI5(YDR146C)|FD-Score:-4.04|P-value:2.66E-5||SGD DESC:Transcription factor that recruits Mediator and Swi/Snf complexes; activates transcription of genes expressed at the M/G1 phase boundary and in G1 phase; required for expression of the HO gene controlling mating type switching; localization to nucleus occurs during G1 and appears to be regulated by phosphorylation by Cdc28p kinase; SWI5 has a paralog, ACE2, that arose from the whole genome duplication Gene:SYP1(YCR030C)|FD-Score:-4.6|P-value:2.14E-6||SGD DESC:Protein of unknown function that is involved in endocytic site formation; may regulate assembly and disassembly of the septin ring; colocalizes and interacts with septin subunits; potential role in actin cytoskeletal organization Gene:TSC3(YBR058C-A)|FD-Score:3.48|P-value:2.46E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VMA1(YDL185W)|FD-Score:3.15|P-value:8.24E-4||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VPS21(YOR089C)|FD-Score:-6.77|P-value:6.48E-12||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS25(YJR102C)|FD-Score:-3.37|P-value:3.78E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:YBL100W-C(YBL100W-C_p)|FD-Score:4.59|P-value:2.23E-6||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YDR290W(YDR290W_d)|FD-Score:-4.25|P-value:1.07E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Gene:YER175W-A(YER175W-A_p)|FD-Score:-3.41|P-value:3.26E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YER184C(YER184C_p)|FD-Score:3.83|P-value:6.45E-5||SGD DESC:Putative zinc cluster protein; deletion confers sensitivity to Calcufluor white, and prevents growth on glycerol or lactate as sole carbon source Gene:YFR006W(YFR006W_p)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Putative X-Pro aminopeptidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YFR006W is not an essential gene Gene:YGL007C-A(YGL007C-A_p)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Putative protein of unknown function, identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; deletion exhibits slow-growth phenotype; computationally predicted to have a role in cell budding Gene:YGR015C(YGR015C_p)|FD-Score:4.56|P-value:2.61E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:YGR127W(YGR127W_p)|FD-Score:5.53|P-value:1.65E-8||SGD DESC:Putative protein of unknown function; expression is regulated by Msn2p/Msn4p, indicating a possible role in stress response Gene:YIL029C(YIL029C_p)|FD-Score:-3.41|P-value:3.28E-4||SGD DESC:Putative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YIL029C has paralog YPR071W Gene:YLR012C(YLR012C_p)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Putative protein of unknown function; YLR012C is not an essential gene Gene:YMR075C-A(YMR075C-A_d)|FD-Score:3.33|P-value:4.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RCO1/YMR075W Gene:YMR175W-A(YMR175W-A_p)|FD-Score:-3.25|P-value:5.75E-4||SGD DESC:Putative protein of unknown function Gene:YNL140C(YNL140C_p)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps THO2/YNL139C Gene:YOL013W-A(YOL013W-A_p)|FD-Score:3.17|P-value:7.55E-4||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YPL014W(YPL014W_p)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus Gene:YRB30(YGL164C)|FD-Score:-4.1|P-value:2.03E-5||SGD DESC:RanGTP-binding protein, inhibits RanGAP1 (Rna1p)-mediated GTP hydrolysis of RanGTP (Gsp1p); shares similarity to proteins in other fungi but not in higher eukaryotes

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YIL068C5.677.02E-90.75SEC6Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p
YGR048W5.511.84E-80.75UFD1Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transaction factors by regulated Ub/proteasome-dependent processing (RUP)
YJL097W4.751.01E-60.07PHS1Essential 3-hydroxyacyl-CoA dehydratase of the ER membrane, involved in elongation of very long-chain fatty acids; evolutionarily conserved, similar to mammalian PTPLA and PTPLB; involved in sphingolipid biosynthesis and protein trafficking
YHR062C4.681.45E-60.17RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YFR002W4.513.31E-60.49NIC96Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p)
YHR164C4.022.92E-50.10DNA2Tripartite DNA replication factor; has single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; required for Okazaki fragment processing; involved in DNA repair; cell-cycle dependent localization; forms nuclear foci upon DNA replication stress
YPR034W3.924.44E-50.19ARP7Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YLR310C3.739.57E-50.07CDC25Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1
YDL152W_d3.661.24E-40.01YDL152W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome
YPR016C3.661.27E-40.04TIF6Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits
YNL306W3.611.51E-40.25MRPS18Mitochondrial ribosomal protein of the small subunit; essential for viability, unlike most other mitoribosomal proteins
YNL062C3.373.80E-40.12GCD10Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression
YGR128C3.255.83E-40.07UTP8Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YGL011C3.187.41E-40.02SCL1Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria
YDR478W3.167.97E-40.09SNM1Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR297W7.416.26E-14SUR2Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis
YJL047C6.729.06E-12RTT101Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p
YOL138C6.602.09E-11RTC1Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; null mutation suppresses cdc13-1 temperature sensitivity; has N-terminal WD-40 repeats and a C-terminal RING motif
YPR043W6.465.38E-11RPL43ARibosomal 60S subunit protein L43A; null mutation confers a dominant lethal phenotype; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43A has a paralog, RPL43B, that arose from the whole genome duplication
YJL149W6.271.79E-10DAS1Putative SCF ubiquitin ligase F-box protein; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C
YGR127W_p5.531.65E-8YGR127W_pPutative protein of unknown function; expression is regulated by Msn2p/Msn4p, indicating a possible role in stress response
YIL159W5.393.45E-8BNR1Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNI1
YJR051W4.963.59E-7OSM1Fumarate reductase, catalyzes the reduction of fumarate to succinate; required for the reoxidation of intracellular NADH under anaerobic conditions; mutations cause osmotic sensitivity; OSM1 has a paralog, FRD1, that arose from the whole genome duplication
YBL100W-C_p4.592.23E-6YBL100W-C_pPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
YGR015C_p4.562.61E-6YGR015C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion
YNL180C4.523.15E-6RHO5Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely involved in protein kinase C (Pkc1p)-dependent signal transduction pathway that controls cell integrity
YLL042C4.261.02E-5ATG10Conserved E2-like conjugating enzyme that mediates formation of the Atg12p-Atg5p conjugate, which is a critical step in autophagy
YBR231C4.241.11E-5SWC5Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress
YPR047W4.131.80E-5MSF1Mitochondrial phenylalanyl-tRNA synthetase, active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase
YPR075C4.072.37E-5OPY2Integral membrane protein that acts as a membrane anchor for Ste50p; involved in the signaling branch of the high-osmolarity glycerol (HOG) pathway and as a regulator of the filamentous growth pathway; overproduction blocks cell cycle arrest in the presence of mating pheromone; relocalizes from vacuole to plasma membrane upon DNA replication stress

GO enrichment analysis for SGTC_2793
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1739.64E-41SGTC_463ikarugamyin 104.0 μMICCB bioactive library54714554NA
0.1171.37E-19SGTC_28007887935 81.8 μMChembridge (Drug-like library)29610020.114286RPP1 & pyrimidine depletion
0.0984.33E-14SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.186441TSC3-RPN4
0.0971.02E-13SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.030303TSC3-RPN4
0.0881.07E-11SGTC_10244507-0395 26.0 μMChemDiv (Drug-like library)42018360.131579TSC3-RPN4
0.0881.61E-11SGTC_2618avocadyne 43.4 μMMicrosource (Natural product library)30151890.0333333
0.0841.27E-10SGTC_26682',4'-dihydroxychalcone 15.1 μMTimTec (Pure natural product library)53572180.1
0.0831.98E-10SGTC_24984'-Methoxychalcone 54.6 μMMicrosource (Natural product library)6418180.175439RPP1 & pyrimidine depletion
0.0807.28E-10SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.118421TSC3-RPN4
0.0807.46E-10SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.0985916TSC3-RPN4
0.0791.30E-9SGTC_1662st014054 52.0 μMTimTec (Natural product derivative library)28387770.128205TSC3-RPN4
0.0765.34E-9SGTC_1845st056753 3.0 μMTimTec (Natural product derivative library)53758390.09375TSC3-RPN4
0.0723.37E-8SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.186441TSC3-RPN4
0.0715.02E-8SGTC_11730443-0020 122.0 μMChemDiv (Drug-like library)53312950.163934TSC3-RPN4
0.0715.18E-8SGTC_10134112-3890 241.0 μMChemDiv (Drug-like library)7051710.150685TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_2882905804458.44 μM0.53061218076804Chembridge (Drug-like library)273.3021433.62513
SGTC_2844901010325.97 μM0.4423082480525Chembridge (Drug-like library)327.321494.01916
SGTC_2886905894451.95 μM0.4313734949929Chembridge (Drug-like library)299.364243.92713
SGTC_3070911700549.47 μM0.4230778779016Chembridge (Drug-like library)273.3021433.5313
SGTC_3098911903949.47 μM0.39285717161528Chembridge (Drug-like library)287.3287234.05313Golgi
SGTC_3150909757449.47 μM0.38333317217476Chembridge (Drug-like library)339.43143.52304
SGTC_3056909490049.47 μM0.36206925236784Chembridge (Drug-like library)342.4072233.88404
SGTC_3290912103171.43 μM0.34920617014854Chembridge (Drug-like library)354.3781634.00915
SGTC_2841900476138.96 μM0.3333332991039Chembridge (Drug-like library)289.756744.08412amide catabolism
SGTC_2856902292519.48 μM0.3275864944862Chembridge (Drug-like library)287.3287234.11213