7949634

methyl 2-(3-phenylpropanoylamino)-4-pyridin-4-ylthiophene-3-carboxylate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2796
Screen concentration 45.5 μM
Source Chembridge (Drug-like library)
PubChem CID 2971588
SMILES COC(=O)C1=C(SC=C1C2=CC=NC=C2)NC(=O)CCC3=CC=CC=C3
Standardized SMILES COC(=O)c1c(NC(=O)CCc2ccccc2)scc1c3ccncc3
Molecular weight 366.4335
ALogP 3.12
H-bond donor count 1
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 5.77
% growth inhibition (Hom. pool) 6.83


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2971588
Download HIP data (tab-delimited text)  (excel)
Gene:CCA1(YER168C)|FD-Score:-3.15|P-value:8.12E-4|Clearance:0||SGD DESC:ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites Gene:CDS1(YBR029C)|FD-Score:3.75|P-value:8.89E-5|Clearance:0.07||SGD DESC:Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids Gene:CWC24(YLR323C)|FD-Score:3.15|P-value:8.23E-4|Clearance:0.07||SGD DESC:Spliceosome assembly factor, essential for the first step of splicing; component of the pre-catalytic spliceosome complex containing Cef1p; has similarity to S. pombe Cwf24p Gene:DPB11(YJL090C)|FD-Score:3.94|P-value:4.11E-5|Clearance:0||SGD DESC:DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress Gene:GPI10(YGL142C)|FD-Score:6.07|P-value:6.56E-10|Clearance:0.99||SGD DESC:Integral membrane protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; putative alpha 1,2 mannosyltransferase required for addition of the third mannose onto the GPI core structure; human PIG-Bp is a functional homolog Gene:HRR25(YPL204W)|FD-Score:-3.3|P-value:4.89E-4|Clearance:0||SGD DESC:Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) Gene:KAR2(YJL034W)|FD-Score:-7.62|P-value:1.30E-14|Clearance:0||SGD DESC:ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p Gene:KEI1(YDR367W)|FD-Score:6.08|P-value:6.13E-10|Clearance:0.99||SGD DESC:Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene Gene:KRE5(YOR336W)|FD-Score:4.3|P-value:8.64E-6|Clearance:0.25||SGD DESC:Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects Gene:LIP1(YMR298W)|FD-Score:-3.31|P-value:4.74E-4|Clearance:0||SGD DESC:Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis Gene:LSG1(YGL099W)|FD-Score:5.07|P-value:1.95E-7|Clearance:0.78||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MSL5(YLR116W)|FD-Score:3.94|P-value:4.15E-5|Clearance:0.02||SGD DESC:Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA Gene:PAP1(YKR002W)|FD-Score:3.27|P-value:5.37E-4|Clearance:0.06||SGD DESC:Poly(A) polymerase, one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs; required for gene looping Gene:PDI1(YCL043C)|FD-Score:3.92|P-value:4.43E-5|Clearance:0.07||SGD DESC:Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication Gene:PHS1(YJL097W)|FD-Score:4.05|P-value:2.59E-5|Clearance:0.11||SGD DESC:Essential 3-hydroxyacyl-CoA dehydratase of the ER membrane, involved in elongation of very long-chain fatty acids; evolutionarily conserved, similar to mammalian PTPLA and PTPLB; involved in sphingolipid biosynthesis and protein trafficking Gene:PRE6(YOL038W)|FD-Score:3.85|P-value:5.94E-5|Clearance:0.09||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:PRT1(YOR361C)|FD-Score:-3.7|P-value:1.09E-4|Clearance:0||SGD DESC:eIF3b subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes Gene:RFA2(YNL312W)|FD-Score:-3.69|P-value:1.10E-4|Clearance:0||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress Gene:RLI1(YDR091C)|FD-Score:-4.22|P-value:1.23E-5|Clearance:0||SGD DESC:Essential iron-sulfur protein required for ribosome biogenesis and translation initiation and termination; facilitates binding of a multifactor complex (MFC) of initiation factors to the small ribosomal subunit; predicted ABC family ATPase; forms a complex with Lto1p and Yae1p that may be involved in protection of the ribosome from damage due to reactive oxygen species Gene:RPC40(YPR110C)|FD-Score:3.68|P-value:1.18E-4|Clearance:0.23||SGD DESC:RNA polymerase subunit AC40, common to RNA polymerase I and III Gene:RPC82(YPR190C)|FD-Score:3.76|P-value:8.56E-5|Clearance:0.01||SGD DESC:RNA polymerase III subunit C82 Gene:RRN7(YJL025W)|FD-Score:-3.1|P-value:9.70E-4|Clearance:0||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:RRP42(YDL111C)|FD-Score:3.21|P-value:6.53E-4|Clearance:0.07||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7) Gene:SEC62(YPL094C)|FD-Score:-3.39|P-value:3.52E-4|Clearance:0||SGD DESC:Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SGT1(YOR057W)|FD-Score:3.44|P-value:2.88E-4|Clearance:0.15||SGD DESC:Cochaperone protein; regulates activity of adenylyl cyclase Cyr1p; involved in kinetochore complex assembly; associates with the SCF (Skp1p/Cdc53p/F box protein) ubiquitin ligase complex; acts as a linker between Skp1p and HSP90 complexes; protein abundance increases in response to DNA replication stress Gene:SSL2(YIL143C)|FD-Score:-4.15|P-value:1.68E-5|Clearance:0||SGD DESC:Component of RNA polymerase transcription factor TFIIH holoenzyme; has DNA-dependent ATPase/helicase activity and is required, with Rad3p, for unwinding promoter DNA; interacts functionally with TFIIB and has roles in transcription start site selection and in gene looping to juxtapose initiation and termination regions; involved in DNA repair; homolog of human ERCC3 Gene:TFB2(YPL122C)|FD-Score:-3.1|P-value:9.59E-4|Clearance:0||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH Gene:TUB1(YML085C)|FD-Score:3.3|P-value:4.91E-4|Clearance:0.03||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:CCA1(YER168C)|FD-Score:-3.15|P-value:8.12E-4|Clearance:0||SGD DESC:ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites Gene:CDS1(YBR029C)|FD-Score:3.75|P-value:8.89E-5|Clearance:0.07||SGD DESC:Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids Gene:CWC24(YLR323C)|FD-Score:3.15|P-value:8.23E-4|Clearance:0.07||SGD DESC:Spliceosome assembly factor, essential for the first step of splicing; component of the pre-catalytic spliceosome complex containing Cef1p; has similarity to S. pombe Cwf24p Gene:DPB11(YJL090C)|FD-Score:3.94|P-value:4.11E-5|Clearance:0||SGD DESC:DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress Gene:GPI10(YGL142C)|FD-Score:6.07|P-value:6.56E-10|Clearance:0.99||SGD DESC:Integral membrane protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; putative alpha 1,2 mannosyltransferase required for addition of the third mannose onto the GPI core structure; human PIG-Bp is a functional homolog Gene:HRR25(YPL204W)|FD-Score:-3.3|P-value:4.89E-4|Clearance:0||SGD DESC:Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) Gene:KAR2(YJL034W)|FD-Score:-7.62|P-value:1.30E-14|Clearance:0||SGD DESC:ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p Gene:KEI1(YDR367W)|FD-Score:6.08|P-value:6.13E-10|Clearance:0.99||SGD DESC:Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene Gene:KRE5(YOR336W)|FD-Score:4.3|P-value:8.64E-6|Clearance:0.25||SGD DESC:Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects Gene:LIP1(YMR298W)|FD-Score:-3.31|P-value:4.74E-4|Clearance:0||SGD DESC:Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis Gene:LSG1(YGL099W)|FD-Score:5.07|P-value:1.95E-7|Clearance:0.78||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MSL5(YLR116W)|FD-Score:3.94|P-value:4.15E-5|Clearance:0.02||SGD DESC:Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA Gene:PAP1(YKR002W)|FD-Score:3.27|P-value:5.37E-4|Clearance:0.06||SGD DESC:Poly(A) polymerase, one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs; required for gene looping Gene:PDI1(YCL043C)|FD-Score:3.92|P-value:4.43E-5|Clearance:0.07||SGD DESC:Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication Gene:PHS1(YJL097W)|FD-Score:4.05|P-value:2.59E-5|Clearance:0.11||SGD DESC:Essential 3-hydroxyacyl-CoA dehydratase of the ER membrane, involved in elongation of very long-chain fatty acids; evolutionarily conserved, similar to mammalian PTPLA and PTPLB; involved in sphingolipid biosynthesis and protein trafficking Gene:PRE6(YOL038W)|FD-Score:3.85|P-value:5.94E-5|Clearance:0.09||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:PRT1(YOR361C)|FD-Score:-3.7|P-value:1.09E-4|Clearance:0||SGD DESC:eIF3b subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes Gene:RFA2(YNL312W)|FD-Score:-3.69|P-value:1.10E-4|Clearance:0||SGD DESC:Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress Gene:RLI1(YDR091C)|FD-Score:-4.22|P-value:1.23E-5|Clearance:0||SGD DESC:Essential iron-sulfur protein required for ribosome biogenesis and translation initiation and termination; facilitates binding of a multifactor complex (MFC) of initiation factors to the small ribosomal subunit; predicted ABC family ATPase; forms a complex with Lto1p and Yae1p that may be involved in protection of the ribosome from damage due to reactive oxygen species Gene:RPC40(YPR110C)|FD-Score:3.68|P-value:1.18E-4|Clearance:0.23||SGD DESC:RNA polymerase subunit AC40, common to RNA polymerase I and III Gene:RPC82(YPR190C)|FD-Score:3.76|P-value:8.56E-5|Clearance:0.01||SGD DESC:RNA polymerase III subunit C82 Gene:RRN7(YJL025W)|FD-Score:-3.1|P-value:9.70E-4|Clearance:0||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:RRP42(YDL111C)|FD-Score:3.21|P-value:6.53E-4|Clearance:0.07||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7) Gene:SEC62(YPL094C)|FD-Score:-3.39|P-value:3.52E-4|Clearance:0||SGD DESC:Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SGT1(YOR057W)|FD-Score:3.44|P-value:2.88E-4|Clearance:0.15||SGD DESC:Cochaperone protein; regulates activity of adenylyl cyclase Cyr1p; involved in kinetochore complex assembly; associates with the SCF (Skp1p/Cdc53p/F box protein) ubiquitin ligase complex; acts as a linker between Skp1p and HSP90 complexes; protein abundance increases in response to DNA replication stress Gene:SSL2(YIL143C)|FD-Score:-4.15|P-value:1.68E-5|Clearance:0||SGD DESC:Component of RNA polymerase transcription factor TFIIH holoenzyme; has DNA-dependent ATPase/helicase activity and is required, with Rad3p, for unwinding promoter DNA; interacts functionally with TFIIB and has roles in transcription start site selection and in gene looping to juxtapose initiation and termination regions; involved in DNA repair; homolog of human ERCC3 Gene:TFB2(YPL122C)|FD-Score:-3.1|P-value:9.59E-4|Clearance:0||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH Gene:TUB1(YML085C)|FD-Score:3.3|P-value:4.91E-4|Clearance:0.03||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2971588
Download HOP data (tab-delimited text)  (excel)
Gene:AIR2(YDL175C)|FD-Score:3.18|P-value:7.35E-4||SGD DESC:RNA-binding subunit of the TRAMP nuclear RNA surveillance complex; involved in nuclear RNA processing and degradation; involved in TRAMP complex assembly as a bridge between Mtr4p and Trf4p; stimulates the poly(A) polymerase activity of Pap2p in vitro; has 5 zinc knuckle motifs; AIR2 has a paralog, AIR1, that arose from the whole genome duplication Gene:AUA1(YFL010W-A)|FD-Score:-3.32|P-value:4.49E-4||SGD DESC:Protein required for the negative regulation by ammonia of Gap1p, which is a general amino acid permease Gene:BER1(YLR412W)|FD-Score:-3.36|P-value:3.84E-4||SGD DESC:Protein involved in microtubule-related processes, N-acetylation; GFP-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YLR412W is not an essential gene; similar to Arabidopsis SRR1 gene Gene:BIT61(YJL058C)|FD-Score:-3.57|P-value:1.76E-4||SGD DESC:Subunit of TORC2 membrane-associated complex; involved in regulation of cell cycle-dependent actin cytoskeletal dynamics during polarized growth and cell wall integrity; BIT61 has a paralog, BIT2, that arose from the whole genome duplication Gene:BLM10(YFL007W)|FD-Score:-4.01|P-value:3.01E-5||SGD DESC:Proteasome activator; binds the core proteasome and stimulates proteasome-mediated protein degradation by inducing gate opening; required for resistance to bleomycin, may be involved in protecting against oxidative damage; similar to mammalian PA200 Gene:BSP1(YPR171W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Adapter that links synaptojanins Inp52p and Inp53p to the cortical actin cytoskeleton Gene:BUD4(YJR092W)|FD-Score:-3.17|P-value:7.75E-4||SGD DESC:Anillin-like protein involved in bud-site selection; required for the axial budding pattern; localizes with septins to the bud neck in mitosis and may constitute an axial landmark for the next round of budding; required for the formation and disassembly of the double septin ring structure, and generally for septin organization; in vivo substrate of Cdc28p/Clb2p Gene:CAT5(YOR125C)|FD-Score:5.69|P-value:6.22E-9||SGD DESC:Protein required for ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes; required for gluconeogenic gene activation Gene:CCS1(YMR038C)|FD-Score:4.15|P-value:1.64E-5||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CIN4(YMR138W)|FD-Score:5.63|P-value:8.81E-9||SGD DESC:GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog Gene:CLD1(YGR110W)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58 Gene:COT1(YOR316C)|FD-Score:3.74|P-value:9.17E-5||SGD DESC:Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistance to cobalt and rhodium; protein abundance increases in response to DNA replication stress; COT1 has a paralog, ZRC1, that arose from the whole genome duplication Gene:CTA1(YDR256C)|FD-Score:5.46|P-value:2.32E-8||SGD DESC:Catalase A, breaks down hydrogen peroxide in the peroxisomal matrix formed by acyl-CoA oxidase (Pox1p) during fatty acid beta-oxidation Gene:DAS2(YDR020C_p)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; weak similarity with uridine kinases and with phosphoribokinases Gene:DIE2(YGR227W)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Dolichyl-phosphoglucose-dependent alpha-1,2 glucosyltransferase of the ER, functions in the pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation, has a role in regulation of ITR1 and INO1 Gene:ECM31(YBR176W)|FD-Score:-3.19|P-value:7.22E-4||SGD DESC:Ketopantoate hydroxymethyltransferase, required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2-dehydropantoate Gene:ETT1(YOR051C)|FD-Score:-3.29|P-value:5.07E-4||SGD DESC:Nuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts; deletion increases stop codon readthrough Gene:FAU1(YER183C)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis Gene:FCJ1(YKR016W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Mitochondrial inner membrane protein, ortholog of mammalian mitofilin; involved in import of intermembrane space (IMS) proteins, probably by positioning Mia40p relative to the TOM complex to receive incoming proteins; also has an essential role in the maintenance of crista junctions and inner membrane architecture, as a component of the mitochondrial inner membrane organizing system (MICOS, MitOS or MINOS), a scaffold-like structure on the IMS side of the inner membrane Gene:FET4(YMR319C)|FD-Score:6.34|P-value:1.17E-10||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:GAS2(YLR343W)|FD-Score:3.22|P-value:6.44E-4||SGD DESC:1,3-beta-glucanosyltransferase, involved with Gas4p in spore wall assembly; has similarity to Gas1p Gene:GCN5(YGR252W)|FD-Score:3.91|P-value:4.63E-5||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GLY1(YEL046C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GRX1(YCL035C)|FD-Score:-3.12|P-value:8.93E-4||SGD DESC:Glutathione-dependent disulfide oxidoreductase; hydroperoxide and superoxide-radical responsive, heat-stable, with active site cysteine pair; protects cells from oxidative damage; GRX1 has a paralog, GRX2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:GTO1(YGR154C)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Omega-class glutathione transferase; induced under oxidative stress; putative peroxisomal localization Gene:HBN1(YCL026C-B_p)|FD-Score:-4.39|P-value:5.74E-6||SGD DESC:Protein of unknown function; similar to bacterial nitroreductases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein becomes insoluble upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:IDP1(YDL066W)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes Gene:IRC6(YFR043C)|FD-Score:-4.36|P-value:6.64E-6||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:KGD2(YDR148C)|FD-Score:3.27|P-value:5.36E-4||SGD DESC:Dihydrolipoyl transsuccinylase, component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes the oxidative decarboxylation of alpha-ketoglutarate to succinyl-CoA in the TCA cycle; phosphorylated Gene:KIN2(YLR096W)|FD-Score:-3.87|P-value:5.44E-5||SGD DESC:Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p Gene:LEM3(YNL323W)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LSM12(YHR121W)|FD-Score:-3.26|P-value:5.66E-4||SGD DESC:Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS; relative distribution to the nucleus increases upon DNA replication stress Gene:MAM3(YOL060C)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:Protein required for normal mitochondrial morphology, has similarity to hemolysins Gene:MEF2(YJL102W)|FD-Score:5.76|P-value:4.16E-9||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MLS1(YNL117W)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:Malate synthase, enzyme of the glyoxylate cycle; involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth on oleic acid, otherwise cytosolic Gene:MOD5(YOR274W)|FD-Score:4.23|P-value:1.18E-5||SGD DESC:Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis Gene:MRM2(YGL136C)|FD-Score:4.28|P-value:9.14E-6||SGD DESC:Mitochondrial 2' O-ribose methyltransferase, required for methylation of U(2791) in 21S rRNA; MRM2 deletion confers thermosensitive respiration and loss of mitochondrial DNA; has similarity to Spb1p and Trm7p, and to E. coli FtsJ/RrmJ Gene:NDT80(YHR124W)|FD-Score:4.67|P-value:1.50E-6||SGD DESC:Meiosis-specific transcription factor required for exit from pachytene and for full meiotic recombination; activates middle sporulation genes; competes with Sum1p for binding to promoters containing middle sporulation elements (MSE) Gene:NFU1(YKL040C)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Protein involved in iron metabolism in mitochondria; similar to NifU, which is a protein required for the maturation of the Fe/S clusters of nitrogenase in nitrogen-fixing bacteria Gene:NUR1(YDL089W)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:OPY2(YPR075C)|FD-Score:3.36|P-value:3.93E-4||SGD DESC:Integral membrane protein that acts as a membrane anchor for Ste50p; involved in the signaling branch of the high-osmolarity glycerol (HOG) pathway and as a regulator of the filamentous growth pathway; overproduction blocks cell cycle arrest in the presence of mating pheromone; relocalizes from vacuole to plasma membrane upon DNA replication stress Gene:PCD1(YLR151C)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:Peroxisomal pyrophosphatase with specificity for coenzyme A and CoA derivatives, may function to remove potentially toxic oxidized CoA disulfide from peroxisomes to maintain the capacity for beta-oxidation of fatty acids; nudix hydrolase family member Gene:PET111(YMR257C)|FD-Score:3.2|P-value:6.88E-4||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:PHO13(YDL236W)|FD-Score:4.11|P-value:1.95E-5||SGD DESC:Alkaline phosphatase specific for p-nitrophenyl phosphate; also has protein phosphatase activity Gene:PTM1(YKL039W)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Protein of unknown function; copurifies with late Golgi vesicles containing the v-SNARE Tlg2p; PTM1 has a paralog, YHL017W, that arose from the whole genome duplication Gene:PUS5(YLR165C)|FD-Score:4.62|P-value:1.87E-6||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine (Psi)-2819 in mitochondrial 21S rRNA; not essential for viability Gene:RAD14(YMR201C)|FD-Score:3.15|P-value:8.08E-4||SGD DESC:Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein Gene:RAV2(YDR202C)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme Gene:REI1(YBR267W)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network Gene:RIC1(YLR039C)|FD-Score:3.37|P-value:3.80E-4||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPL40B(YKR094C)|FD-Score:3.13|P-value:8.69E-4||SGD DESC:Ubiquitin-ribosomal 60S subunit protein L40B fusion protein; cleaved to yield ubiquitin and ribosomal protein L40B; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40B has a paralog, RPL40A, that arose from the whole genome duplication Gene:RSM25(YIL093C)|FD-Score:4.21|P-value:1.25E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:SFB3(YHR098C)|FD-Score:-4.58|P-value:2.34E-6||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SFH5(YJL145W)|FD-Score:4.03|P-value:2.75E-5||SGD DESC:Non-classical phosphatidylinositol transfer protein (PITP); exhibits PI- but not PC-transfer activity; localizes to the peripheral endoplasmic reticulum, cytosol and microsomes; similar to Sec14p; partially relocalizes to the plasma membrane upon DNA replication stress Gene:SKY1(YMR216C)|FD-Score:3.29|P-value:5.06E-4||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SPT10(YJL127C)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Putative histone acetylase with a role in transcriptional silencing, sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box Gene:SUR2(YDR297W)|FD-Score:-4.12|P-value:1.93E-5||SGD DESC:Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis Gene:SWA2(YDR320C)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:SWI3(YJL176C)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; SWI/SNF regulates transcription by remodeling chromosomes; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; relocates to the cytosol under hypoxic conditions Gene:TOS8(YGL096W)|FD-Score:3.59|P-value:1.62E-4||SGD DESC:Homeodomain-containing protein and putative transcription factor; found associated with chromatin; target of SBF transcription factor; induced during meiosis and under cell-damaging conditions; TOS8 has a paralog, CUP9, that arose from the whole genome duplication Gene:TSC3(YBR058C-A)|FD-Score:-3.25|P-value:5.87E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:ULA1(YPL003W)|FD-Score:7.35|P-value:9.73E-14||SGD DESC:Protein that acts together with Uba3p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation Gene:VID24(YBR105C)|FD-Score:-5.09|P-value:1.76E-7||SGD DESC:GID Complex regulatory subunit; binds GID Complex in response to glucose through interactions with complex member Vid28p; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; promotes proteasome-dependent catabolite degradation of FBPase; peripheral membrane protein located at Vid (vacuole import and degradation) vesicles Gene:VPS21(YOR089C)|FD-Score:5.65|P-value:8.19E-9||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:YAP1801(YHR161C)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1801 has a paralog, YAP1802, that arose from the whole genome duplication Gene:YBR196C-B(YBR196C-B_p)|FD-Score:3.27|P-value:5.37E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YCK3(YER123W)|FD-Score:-4.7|P-value:1.28E-6||SGD DESC:Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway Gene:YCR007C(YCR007C_p)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene Gene:YDR537C(YDR537C_d)|FD-Score:-5.32|P-value:5.30E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, almost completely overlaps verified ORF PAD1/YDR538W Gene:YGL101W(YGL101W_p)|FD-Score:-3.3|P-value:4.81E-4||SGD DESC:Putative protein of unknown function; non-essential gene with similarity to YBR242W; interacts with the DNA helicase Hpr5p Gene:YGR122C-A(YGR122C-A_d)|FD-Score:-3.47|P-value:2.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, similar to YLR334C and YOL106W Gene:YGR127W(YGR127W_p)|FD-Score:11.4|P-value:1.48E-30||SGD DESC:Putative protein of unknown function; expression is regulated by Msn2p/Msn4p, indicating a possible role in stress response Gene:YIL060W(YIL060W_p)|FD-Score:3.73|P-value:9.56E-5||SGD DESC:Putative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene Gene:YJR011C(YJR011C_p)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:Putative protein of unknown function; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS Gene:YJR111C(YJR111C_p)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria Gene:YKL169C(YKL169C_d)|FD-Score:3.17|P-value:7.50E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YKR070W(YKR070W_p)|FD-Score:3.24|P-value:5.94E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLL020C(YLL020C_d)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KNS1 Gene:YLR012C(YLR012C_p)|FD-Score:5.07|P-value:1.97E-7||SGD DESC:Putative protein of unknown function; YLR012C is not an essential gene Gene:YML094C-A(YML094C-A_d)|FD-Score:-4.53|P-value:2.95E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO Gene:YNL108C(YNL108C_p)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Putative protein of unknown function; similar to prokaryotic phosphotransfer enzymes; null mutant shows alterations in glucose metabolism; GFP-fusion protein localizes to the cytoplasm and nucleus; YNL108C has a paralog, TFC7, that arose from the whole genome duplication Gene:YNL140C(YNL140C_p)|FD-Score:-6.18|P-value:3.14E-10||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps THO2/YNL139C Gene:YOL159C(YOL159C)|FD-Score:-3.18|P-value:7.42E-4||SGD DESC:Soluble protein of unknown function; deletion mutants are viable and have elevated levels of Ty1 retrotransposition and Ty1 cDNA Gene:YOR293C-A(YOR293C-A_p)|FD-Score:-3.48|P-value:2.52E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YPC1(YBR183W)|FD-Score:-3.49|P-value:2.40E-4||SGD DESC:Alkaline ceramidase; also has reverse (CoA-independent) ceramide synthase activity; catalyzes both breakdown and synthesis of phytoceramide; overexpression confers fumonisin B1 resistance; YPC1 has a paralog, YDC1, that arose from the whole genome duplication Gene:YPK9(YOR291W)|FD-Score:4.24|P-value:1.11E-5||SGD DESC:Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-type ATPase Spf1p; homolog of human ATP13A2 (PARK9), mutations in which are associated with Parkinson disease and Kufor-Rakeb syndrome Gene:YPL205C(YPL205C_d)|FD-Score:3.1|P-value:9.70E-4||SGD DESC:Hypothetical protein; deletion of locus affects telomere length Gene:YPL245W(YPL245W_p)|FD-Score:3.19|P-value:7.02E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm Gene:YRF1-6(YNL339C)|FD-Score:-3.63|P-value:1.42E-4||SGD DESC:Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p Gene:AIR2(YDL175C)|FD-Score:3.18|P-value:7.35E-4||SGD DESC:RNA-binding subunit of the TRAMP nuclear RNA surveillance complex; involved in nuclear RNA processing and degradation; involved in TRAMP complex assembly as a bridge between Mtr4p and Trf4p; stimulates the poly(A) polymerase activity of Pap2p in vitro; has 5 zinc knuckle motifs; AIR2 has a paralog, AIR1, that arose from the whole genome duplication Gene:AUA1(YFL010W-A)|FD-Score:-3.32|P-value:4.49E-4||SGD DESC:Protein required for the negative regulation by ammonia of Gap1p, which is a general amino acid permease Gene:BER1(YLR412W)|FD-Score:-3.36|P-value:3.84E-4||SGD DESC:Protein involved in microtubule-related processes, N-acetylation; GFP-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YLR412W is not an essential gene; similar to Arabidopsis SRR1 gene Gene:BIT61(YJL058C)|FD-Score:-3.57|P-value:1.76E-4||SGD DESC:Subunit of TORC2 membrane-associated complex; involved in regulation of cell cycle-dependent actin cytoskeletal dynamics during polarized growth and cell wall integrity; BIT61 has a paralog, BIT2, that arose from the whole genome duplication Gene:BLM10(YFL007W)|FD-Score:-4.01|P-value:3.01E-5||SGD DESC:Proteasome activator; binds the core proteasome and stimulates proteasome-mediated protein degradation by inducing gate opening; required for resistance to bleomycin, may be involved in protecting against oxidative damage; similar to mammalian PA200 Gene:BSP1(YPR171W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Adapter that links synaptojanins Inp52p and Inp53p to the cortical actin cytoskeleton Gene:BUD4(YJR092W)|FD-Score:-3.17|P-value:7.75E-4||SGD DESC:Anillin-like protein involved in bud-site selection; required for the axial budding pattern; localizes with septins to the bud neck in mitosis and may constitute an axial landmark for the next round of budding; required for the formation and disassembly of the double septin ring structure, and generally for septin organization; in vivo substrate of Cdc28p/Clb2p Gene:CAT5(YOR125C)|FD-Score:5.69|P-value:6.22E-9||SGD DESC:Protein required for ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes; required for gluconeogenic gene activation Gene:CCS1(YMR038C)|FD-Score:4.15|P-value:1.64E-5||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CIN4(YMR138W)|FD-Score:5.63|P-value:8.81E-9||SGD DESC:GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog Gene:CLD1(YGR110W)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58 Gene:COT1(YOR316C)|FD-Score:3.74|P-value:9.17E-5||SGD DESC:Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistance to cobalt and rhodium; protein abundance increases in response to DNA replication stress; COT1 has a paralog, ZRC1, that arose from the whole genome duplication Gene:CTA1(YDR256C)|FD-Score:5.46|P-value:2.32E-8||SGD DESC:Catalase A, breaks down hydrogen peroxide in the peroxisomal matrix formed by acyl-CoA oxidase (Pox1p) during fatty acid beta-oxidation Gene:DAS2(YDR020C_p)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; weak similarity with uridine kinases and with phosphoribokinases Gene:DIE2(YGR227W)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Dolichyl-phosphoglucose-dependent alpha-1,2 glucosyltransferase of the ER, functions in the pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation, has a role in regulation of ITR1 and INO1 Gene:ECM31(YBR176W)|FD-Score:-3.19|P-value:7.22E-4||SGD DESC:Ketopantoate hydroxymethyltransferase, required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2-dehydropantoate Gene:ETT1(YOR051C)|FD-Score:-3.29|P-value:5.07E-4||SGD DESC:Nuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts; deletion increases stop codon readthrough Gene:FAU1(YER183C)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis Gene:FCJ1(YKR016W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Mitochondrial inner membrane protein, ortholog of mammalian mitofilin; involved in import of intermembrane space (IMS) proteins, probably by positioning Mia40p relative to the TOM complex to receive incoming proteins; also has an essential role in the maintenance of crista junctions and inner membrane architecture, as a component of the mitochondrial inner membrane organizing system (MICOS, MitOS or MINOS), a scaffold-like structure on the IMS side of the inner membrane Gene:FET4(YMR319C)|FD-Score:6.34|P-value:1.17E-10||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:GAS2(YLR343W)|FD-Score:3.22|P-value:6.44E-4||SGD DESC:1,3-beta-glucanosyltransferase, involved with Gas4p in spore wall assembly; has similarity to Gas1p Gene:GCN5(YGR252W)|FD-Score:3.91|P-value:4.63E-5||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GLY1(YEL046C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GRX1(YCL035C)|FD-Score:-3.12|P-value:8.93E-4||SGD DESC:Glutathione-dependent disulfide oxidoreductase; hydroperoxide and superoxide-radical responsive, heat-stable, with active site cysteine pair; protects cells from oxidative damage; GRX1 has a paralog, GRX2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:GTO1(YGR154C)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Omega-class glutathione transferase; induced under oxidative stress; putative peroxisomal localization Gene:HBN1(YCL026C-B_p)|FD-Score:-4.39|P-value:5.74E-6||SGD DESC:Protein of unknown function; similar to bacterial nitroreductases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein becomes insoluble upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:IDP1(YDL066W)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes Gene:IRC6(YFR043C)|FD-Score:-4.36|P-value:6.64E-6||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:KGD2(YDR148C)|FD-Score:3.27|P-value:5.36E-4||SGD DESC:Dihydrolipoyl transsuccinylase, component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes the oxidative decarboxylation of alpha-ketoglutarate to succinyl-CoA in the TCA cycle; phosphorylated Gene:KIN2(YLR096W)|FD-Score:-3.87|P-value:5.44E-5||SGD DESC:Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p Gene:LEM3(YNL323W)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LSM12(YHR121W)|FD-Score:-3.26|P-value:5.66E-4||SGD DESC:Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS; relative distribution to the nucleus increases upon DNA replication stress Gene:MAM3(YOL060C)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:Protein required for normal mitochondrial morphology, has similarity to hemolysins Gene:MEF2(YJL102W)|FD-Score:5.76|P-value:4.16E-9||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MLS1(YNL117W)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:Malate synthase, enzyme of the glyoxylate cycle; involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth on oleic acid, otherwise cytosolic Gene:MOD5(YOR274W)|FD-Score:4.23|P-value:1.18E-5||SGD DESC:Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis Gene:MRM2(YGL136C)|FD-Score:4.28|P-value:9.14E-6||SGD DESC:Mitochondrial 2' O-ribose methyltransferase, required for methylation of U(2791) in 21S rRNA; MRM2 deletion confers thermosensitive respiration and loss of mitochondrial DNA; has similarity to Spb1p and Trm7p, and to E. coli FtsJ/RrmJ Gene:NDT80(YHR124W)|FD-Score:4.67|P-value:1.50E-6||SGD DESC:Meiosis-specific transcription factor required for exit from pachytene and for full meiotic recombination; activates middle sporulation genes; competes with Sum1p for binding to promoters containing middle sporulation elements (MSE) Gene:NFU1(YKL040C)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Protein involved in iron metabolism in mitochondria; similar to NifU, which is a protein required for the maturation of the Fe/S clusters of nitrogenase in nitrogen-fixing bacteria Gene:NUR1(YDL089W)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:OPY2(YPR075C)|FD-Score:3.36|P-value:3.93E-4||SGD DESC:Integral membrane protein that acts as a membrane anchor for Ste50p; involved in the signaling branch of the high-osmolarity glycerol (HOG) pathway and as a regulator of the filamentous growth pathway; overproduction blocks cell cycle arrest in the presence of mating pheromone; relocalizes from vacuole to plasma membrane upon DNA replication stress Gene:PCD1(YLR151C)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:Peroxisomal pyrophosphatase with specificity for coenzyme A and CoA derivatives, may function to remove potentially toxic oxidized CoA disulfide from peroxisomes to maintain the capacity for beta-oxidation of fatty acids; nudix hydrolase family member Gene:PET111(YMR257C)|FD-Score:3.2|P-value:6.88E-4||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:PHO13(YDL236W)|FD-Score:4.11|P-value:1.95E-5||SGD DESC:Alkaline phosphatase specific for p-nitrophenyl phosphate; also has protein phosphatase activity Gene:PTM1(YKL039W)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Protein of unknown function; copurifies with late Golgi vesicles containing the v-SNARE Tlg2p; PTM1 has a paralog, YHL017W, that arose from the whole genome duplication Gene:PUS5(YLR165C)|FD-Score:4.62|P-value:1.87E-6||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine (Psi)-2819 in mitochondrial 21S rRNA; not essential for viability Gene:RAD14(YMR201C)|FD-Score:3.15|P-value:8.08E-4||SGD DESC:Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein Gene:RAV2(YDR202C)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme Gene:REI1(YBR267W)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network Gene:RIC1(YLR039C)|FD-Score:3.37|P-value:3.80E-4||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPL40B(YKR094C)|FD-Score:3.13|P-value:8.69E-4||SGD DESC:Ubiquitin-ribosomal 60S subunit protein L40B fusion protein; cleaved to yield ubiquitin and ribosomal protein L40B; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40B has a paralog, RPL40A, that arose from the whole genome duplication Gene:RSM25(YIL093C)|FD-Score:4.21|P-value:1.25E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:SFB3(YHR098C)|FD-Score:-4.58|P-value:2.34E-6||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SFH5(YJL145W)|FD-Score:4.03|P-value:2.75E-5||SGD DESC:Non-classical phosphatidylinositol transfer protein (PITP); exhibits PI- but not PC-transfer activity; localizes to the peripheral endoplasmic reticulum, cytosol and microsomes; similar to Sec14p; partially relocalizes to the plasma membrane upon DNA replication stress Gene:SKY1(YMR216C)|FD-Score:3.29|P-value:5.06E-4||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SPT10(YJL127C)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Putative histone acetylase with a role in transcriptional silencing, sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box Gene:SUR2(YDR297W)|FD-Score:-4.12|P-value:1.93E-5||SGD DESC:Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis Gene:SWA2(YDR320C)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:SWI3(YJL176C)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; SWI/SNF regulates transcription by remodeling chromosomes; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; relocates to the cytosol under hypoxic conditions Gene:TOS8(YGL096W)|FD-Score:3.59|P-value:1.62E-4||SGD DESC:Homeodomain-containing protein and putative transcription factor; found associated with chromatin; target of SBF transcription factor; induced during meiosis and under cell-damaging conditions; TOS8 has a paralog, CUP9, that arose from the whole genome duplication Gene:TSC3(YBR058C-A)|FD-Score:-3.25|P-value:5.87E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:ULA1(YPL003W)|FD-Score:7.35|P-value:9.73E-14||SGD DESC:Protein that acts together with Uba3p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation Gene:VID24(YBR105C)|FD-Score:-5.09|P-value:1.76E-7||SGD DESC:GID Complex regulatory subunit; binds GID Complex in response to glucose through interactions with complex member Vid28p; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; promotes proteasome-dependent catabolite degradation of FBPase; peripheral membrane protein located at Vid (vacuole import and degradation) vesicles Gene:VPS21(YOR089C)|FD-Score:5.65|P-value:8.19E-9||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:YAP1801(YHR161C)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1801 has a paralog, YAP1802, that arose from the whole genome duplication Gene:YBR196C-B(YBR196C-B_p)|FD-Score:3.27|P-value:5.37E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YCK3(YER123W)|FD-Score:-4.7|P-value:1.28E-6||SGD DESC:Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway Gene:YCR007C(YCR007C_p)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene Gene:YDR537C(YDR537C_d)|FD-Score:-5.32|P-value:5.30E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, almost completely overlaps verified ORF PAD1/YDR538W Gene:YGL101W(YGL101W_p)|FD-Score:-3.3|P-value:4.81E-4||SGD DESC:Putative protein of unknown function; non-essential gene with similarity to YBR242W; interacts with the DNA helicase Hpr5p Gene:YGR122C-A(YGR122C-A_d)|FD-Score:-3.47|P-value:2.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, similar to YLR334C and YOL106W Gene:YGR127W(YGR127W_p)|FD-Score:11.4|P-value:1.48E-30||SGD DESC:Putative protein of unknown function; expression is regulated by Msn2p/Msn4p, indicating a possible role in stress response Gene:YIL060W(YIL060W_p)|FD-Score:3.73|P-value:9.56E-5||SGD DESC:Putative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene Gene:YJR011C(YJR011C_p)|FD-Score:3.24|P-value:6.07E-4||SGD DESC:Putative protein of unknown function; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS Gene:YJR111C(YJR111C_p)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria Gene:YKL169C(YKL169C_d)|FD-Score:3.17|P-value:7.50E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YKR070W(YKR070W_p)|FD-Score:3.24|P-value:5.94E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLL020C(YLL020C_d)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KNS1 Gene:YLR012C(YLR012C_p)|FD-Score:5.07|P-value:1.97E-7||SGD DESC:Putative protein of unknown function; YLR012C is not an essential gene Gene:YML094C-A(YML094C-A_d)|FD-Score:-4.53|P-value:2.95E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO Gene:YNL108C(YNL108C_p)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Putative protein of unknown function; similar to prokaryotic phosphotransfer enzymes; null mutant shows alterations in glucose metabolism; GFP-fusion protein localizes to the cytoplasm and nucleus; YNL108C has a paralog, TFC7, that arose from the whole genome duplication Gene:YNL140C(YNL140C_p)|FD-Score:-6.18|P-value:3.14E-10||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps THO2/YNL139C Gene:YOL159C(YOL159C)|FD-Score:-3.18|P-value:7.42E-4||SGD DESC:Soluble protein of unknown function; deletion mutants are viable and have elevated levels of Ty1 retrotransposition and Ty1 cDNA Gene:YOR293C-A(YOR293C-A_p)|FD-Score:-3.48|P-value:2.52E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YPC1(YBR183W)|FD-Score:-3.49|P-value:2.40E-4||SGD DESC:Alkaline ceramidase; also has reverse (CoA-independent) ceramide synthase activity; catalyzes both breakdown and synthesis of phytoceramide; overexpression confers fumonisin B1 resistance; YPC1 has a paralog, YDC1, that arose from the whole genome duplication Gene:YPK9(YOR291W)|FD-Score:4.24|P-value:1.11E-5||SGD DESC:Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-type ATPase Spf1p; homolog of human ATP13A2 (PARK9), mutations in which are associated with Parkinson disease and Kufor-Rakeb syndrome Gene:YPL205C(YPL205C_d)|FD-Score:3.1|P-value:9.70E-4||SGD DESC:Hypothetical protein; deletion of locus affects telomere length Gene:YPL245W(YPL245W_p)|FD-Score:3.19|P-value:7.02E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm Gene:YRF1-6(YNL339C)|FD-Score:-3.63|P-value:1.42E-4||SGD DESC:Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR367W6.086.13E-100.99KEI1Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene
YGL142C6.076.56E-100.99GPI10Integral membrane protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; putative alpha 1,2 mannosyltransferase required for addition of the third mannose onto the GPI core structure; human PIG-Bp is a functional homolog
YGL099W5.071.95E-70.78LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YOR336W4.308.64E-60.25KRE5Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects
YJL097W4.052.59E-50.11PHS1Essential 3-hydroxyacyl-CoA dehydratase of the ER membrane, involved in elongation of very long-chain fatty acids; evolutionarily conserved, similar to mammalian PTPLA and PTPLB; involved in sphingolipid biosynthesis and protein trafficking
YJL090C3.944.11E-50.00DPB11DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress
YLR116W3.944.15E-50.02MSL5Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA
YCL043C3.924.43E-50.07PDI1Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication
YOL038W3.855.94E-50.09PRE6Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress
YPR190C3.768.56E-50.01RPC82RNA polymerase III subunit C82
YBR029C3.758.89E-50.07CDS1Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids
YPR110C3.681.18E-40.23RPC40RNA polymerase subunit AC40, common to RNA polymerase I and III
YOR057W3.442.88E-40.15SGT1Cochaperone protein; regulates activity of adenylyl cyclase Cyr1p; involved in kinetochore complex assembly; associates with the SCF (Skp1p/Cdc53p/F box protein) ubiquitin ligase complex; acts as a linker between Skp1p and HSP90 complexes; protein abundance increases in response to DNA replication stress
YML085C3.304.91E-40.03TUB1Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication
YKR002W3.275.37E-40.06PAP1Poly(A) polymerase, one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs; required for gene looping

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGR127W_p11.401.48E-30YGR127W_pPutative protein of unknown function; expression is regulated by Msn2p/Msn4p, indicating a possible role in stress response
YPL003W7.359.73E-14ULA1Protein that acts together with Uba3p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation
YMR319C6.341.17E-10FET4Low-affinity Fe(II) transporter of the plasma membrane
YJL102W5.764.16E-9MEF2Mitochondrial elongation factor involved in translational elongation
YOR125C5.696.22E-9CAT5Protein required for ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes; required for gluconeogenic gene activation
YOR089C5.658.19E-9VPS21Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication
YMR138W5.638.81E-9CIN4GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog
YDR256C5.462.32E-8CTA1Catalase A, breaks down hydrogen peroxide in the peroxisomal matrix formed by acyl-CoA oxidase (Pox1p) during fatty acid beta-oxidation
YLR012C_p5.071.97E-7YLR012C_pPutative protein of unknown function; YLR012C is not an essential gene
YHR124W4.671.50E-6NDT80Meiosis-specific transcription factor required for exit from pachytene and for full meiotic recombination; activates middle sporulation genes; competes with Sum1p for binding to promoters containing middle sporulation elements (MSE)
YLR165C4.621.87E-6PUS5Pseudouridine synthase, catalyzes only the formation of pseudouridine (Psi)-2819 in mitochondrial 21S rRNA; not essential for viability
YGL136C4.289.14E-6MRM2Mitochondrial 2' O-ribose methyltransferase, required for methylation of U(2791) in 21S rRNA; MRM2 deletion confers thermosensitive respiration and loss of mitochondrial DNA; has similarity to Spb1p and Trm7p, and to E. coli FtsJ/RrmJ
YOR291W4.241.11E-5YPK9Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-type ATPase Spf1p; homolog of human ATP13A2 (PARK9), mutations in which are associated with Parkinson disease and Kufor-Rakeb syndrome
YOR274W4.231.18E-5MOD5Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis
YIL093C4.211.25E-5RSM25Mitochondrial ribosomal protein of the small subunit

GO enrichment analysis for SGTC_2796
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0808.47E-10SGTC_2420aureobasidin a 50.0 nMMiscellaneous99634300.0909091
0.0781.87E-9SGTC_9761348-1067 47.6 μMChemDiv (Drug-like library)53106710.089743660S ribosome export
0.0681.45E-7SGTC_1958st076513 41.9 μMTimTec (Natural product derivative library)7321220.090909160S ribosome export
0.0656.84E-7SGTC_20525265182 7.1 μMChembridge (Fragment library)12398840.0555556
0.0649.60E-7SGTC_623k072-0108 16.9 μMChemDiv (Drug-like library)53828030.169014heme biosynthesis & mitochondrial translocase
0.0631.38E-6SGTC_2567aureobasidin a 60.0 nMMiscellaneous99634300.0909091
0.0631.46E-6SGTC_1733st037357 54.9 μMTimTec (Natural product derivative library)12723390.14457860S ribosome export
0.0621.80E-6SGTC_620180-0423 3.2 μMChemDiv (Drug-like library)301020.09756160S ribosome export
0.0612.63E-6SGTC_7651319-0117 244.0 μMChemDiv (Drug-like library)34877460.1276660S ribosome export
0.0542.89E-5SGTC_22067254556 147.0 μMChembridge (Fragment library)43989050.25373160S ribosome export
0.0543.45E-5SGTC_1775st081588 35.2 μMTimTec (Natural product derivative library)14264430.10112460S ribosome export
0.0543.76E-5SGTC_1101ebselen 5.3 μMNIH Clinical Collection31940.123077
0.0534.20E-5SGTC_29839022671 13.0 μMChembridge (Drug-like library)64051610.084210560S ribosome export
0.0534.82E-5SGTC_618k072-0232 33.2 μMChemDiv (Drug-like library)58727560.11688360S ribosome export
0.0525.56E-5SGTC_1070indatraline 8.8 μMNIH Clinical Collection103144720.094594660S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3207911416349.47 μM0.365079849233Chembridge (Drug-like library)269.338263.43812
SGTC_2831799903619.48 μM0.3636362988270Chembridge (Drug-like library)323.309654.03115
SGTC_2839900395125.97 μM0.3593752990634Chembridge (Drug-like library)283.364843.81512
SGTC_3082911700849.47 μM0.35483941448529Chembridge (Drug-like library)240.300342.44112
SGTC_9861493-048384 μM0.34426240079ChemDiv (Drug-like library)256.256681.31914
SGTC_3105912104049.47 μM0.33846241448907Chembridge (Drug-like library)268.35353.14912
SGTC_3175910651749.47 μM0.33846241468073Chembridge (Drug-like library)282.380083.35512iron homeostasis
SGTC_22877945887168.79 μM0.3333331245853Chembridge (Fragment library)213.253541.25914
SGTC_2984902210938.96 μM0.3230776459207Chembridge (Drug-like library)269.338263.57512
SGTC_2985902237571.43 μM0.3188414939335Chembridge (Drug-like library)350.454043.68812RPP1 & pyrimidine depletion