7946468

N-[3-(morpholine-4-carbonyl)thiophen-2-yl]-4,5,6,7,8,9-hexahydrocycloocta[b]thiophene-2-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2808
Screen concentration 45.5 μM
Source Chembridge (Drug-like library)
PubChem CID 2970879
SMILES C1CCCC2=C(CC1)C=C(S2)C(=O)NC3=C(C=CS3)C(=O)N4CCOCC4
Standardized SMILES O=C(Nc1sccc1C(=O)N2CCOCC2)c3cc4CCCCCCc4s3
Molecular weight 404.5462
ALogP 3.75
H-bond donor count 1
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.32
% growth inhibition (Hom. pool) 2.47


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2970879
Download HIP data (tab-delimited text)  (excel)
Gene:BFR2(YDR299W)|FD-Score:3.41|P-value:3.28E-4|Clearance:0||SGD DESC:Essential protein that is a component of 90S preribosomes; may be involved in rRNA processing; multicopy suppressor of sensitivity to Brefeldin A; expression is induced during lag phase and also by cold shock Gene:DCP2(YNL118C)|FD-Score:4.64|P-value:1.74E-6|Clearance:0.22||SGD DESC:Catalytic subunit of the Dcp1p-Dcp2p decapping enzyme complex; removes the 5' cap structure from mRNAs prior to their degradation; nudix hydrolase family member; forms cytoplasmic foci upon DNA replication stress Gene:EMG1(YLR186W)|FD-Score:-3.43|P-value:3.01E-4|Clearance:0||SGD DESC:Methyltransferase for rRNA; catalyzes methylation of the pseudouridine residue 1191 of 18S rRNA; member of the SPOUT methyltransferase family; required for maturation of 18S rRNA and for 40S ribosomal subunit production independently of methyltransferase activity; forms homodimers; human ortholog is mutated in Bowen-Conradi syndrome, and the equivalent mutation in yeast affects Emg1p dimerization and localization but not its methyltransferase activity Gene:ERV1(YGR029W)|FD-Score:3.64|P-value:1.38E-4|Clearance:0.04||SGD DESC:Flavin-linked sulfhydryl oxidase of the mitochondrial intermembrane space (IMS), oxidizes Mia40p as part of a disulfide relay system that promotes IMS retention of imported proteins; ortholog of human hepatopoietin (ALR) Gene:GCD1(YOR260W)|FD-Score:3.66|P-value:1.28E-4|Clearance:0.02||SGD DESC:Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GPM1(YKL152C)|FD-Score:-3.67|P-value:1.22E-4|Clearance:0||SGD DESC:Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis Gene:KRR1(YCL059C)|FD-Score:-3.19|P-value:7.09E-4|Clearance:0||SGD DESC:Essential nucleolar protein required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit Gene:NUP82(YJL061W)|FD-Score:3.23|P-value:6.24E-4|Clearance:0.11||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p) Gene:ORC2(YBR060C)|FD-Score:-4.49|P-value:3.63E-6|Clearance:0||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; interacts with Spp1p and with trimethylated histone H3; phosphorylated by Cdc28p Gene:OST2(YOR103C)|FD-Score:-4.86|P-value:5.88E-7|Clearance:0||SGD DESC:Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:POL2(YNL262W)|FD-Score:4.42|P-value:4.95E-6|Clearance:0.21||SGD DESC:Catalytic subunit of DNA polymerase (II) epsilon, a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p Gene:PRP11(YDL043C)|FD-Score:-3.3|P-value:4.90E-4|Clearance:0||SGD DESC:Subunit of the SF3a splicing factor complex, required for spliceosome assembly Gene:RET2(YFR051C)|FD-Score:3.54|P-value:1.97E-4|Clearance:0.14||SGD DESC:Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RPB7(YDR404C)|FD-Score:3.91|P-value:4.62E-5|Clearance:0.18||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPC17(YJL011C)|FD-Score:3.66|P-value:1.24E-4|Clearance:0.01||SGD DESC:RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress Gene:SCD5(YOR329C)|FD-Score:5.31|P-value:5.49E-8|Clearance:0.67||SGD DESC:Protein required for normal actin organization and endocytosis; targeting subunit for protein phosphatase type 1; undergoes Crm1p-dependent nuclear-cytoplasmic shuttling; multicopy suppressor of clathrin deficiency Gene:SEC17(YBL050W)|FD-Score:3.28|P-value:5.13E-4|Clearance:0.06||SGD DESC:Peripheral membrane protein required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, and autophagy; stimulates the ATPase activity of Sec18p; has similarity to mammalian alpha-SNAP Gene:SMC4(YLR086W)|FD-Score:3.59|P-value:1.68E-4|Clearance:0.04||SGD DESC:Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate Gene:SMT3(YDR510W)|FD-Score:3.73|P-value:9.52E-5|Clearance:0.03||SGD DESC:Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2 Gene:SNU13(YEL026W)|FD-Score:3.7|P-value:1.07E-4|Clearance:0.04||SGD DESC:RNA binding protein, part of U3 snoRNP involved in rRNA processing, part of U4/U6-U5 tri-snRNP involved in mRNA splicing, similar to human 15.5K protein Gene:TSR4(YOL022C)|FD-Score:3.59|P-value:1.63E-4|Clearance:0.01||SGD DESC:Cytoplasmic protein required for correct processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; essential gene in S288C background but not in CEN.PK2 Gene:UTP15(YMR093W)|FD-Score:3.11|P-value:9.25E-4|Clearance:0.07||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YGR190C(YGR190C_d)|FD-Score:-3.27|P-value:5.35E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W Gene:YHC1(YLR298C)|FD-Score:-3.14|P-value:8.32E-4|Clearance:0||SGD DESC:Component of the U1 snRNP complex required for pre-mRNA splicing; putative ortholog of human U1C protein, which is involved in formation of a complex between U1 snRNP and the pre-mRNA 5' splice site Gene:YKL083W(YKL083W_d)|FD-Score:4.21|P-value:1.28E-5|Clearance:0.3||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene RRP14 Gene:YOR146W(YOR146W_d)|FD-Score:3.41|P-value:3.29E-4|Clearance:0.12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C Gene:BFR2(YDR299W)|FD-Score:3.41|P-value:3.28E-4|Clearance:0||SGD DESC:Essential protein that is a component of 90S preribosomes; may be involved in rRNA processing; multicopy suppressor of sensitivity to Brefeldin A; expression is induced during lag phase and also by cold shock Gene:DCP2(YNL118C)|FD-Score:4.64|P-value:1.74E-6|Clearance:0.22||SGD DESC:Catalytic subunit of the Dcp1p-Dcp2p decapping enzyme complex; removes the 5' cap structure from mRNAs prior to their degradation; nudix hydrolase family member; forms cytoplasmic foci upon DNA replication stress Gene:EMG1(YLR186W)|FD-Score:-3.43|P-value:3.01E-4|Clearance:0||SGD DESC:Methyltransferase for rRNA; catalyzes methylation of the pseudouridine residue 1191 of 18S rRNA; member of the SPOUT methyltransferase family; required for maturation of 18S rRNA and for 40S ribosomal subunit production independently of methyltransferase activity; forms homodimers; human ortholog is mutated in Bowen-Conradi syndrome, and the equivalent mutation in yeast affects Emg1p dimerization and localization but not its methyltransferase activity Gene:ERV1(YGR029W)|FD-Score:3.64|P-value:1.38E-4|Clearance:0.04||SGD DESC:Flavin-linked sulfhydryl oxidase of the mitochondrial intermembrane space (IMS), oxidizes Mia40p as part of a disulfide relay system that promotes IMS retention of imported proteins; ortholog of human hepatopoietin (ALR) Gene:GCD1(YOR260W)|FD-Score:3.66|P-value:1.28E-4|Clearance:0.02||SGD DESC:Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GPM1(YKL152C)|FD-Score:-3.67|P-value:1.22E-4|Clearance:0||SGD DESC:Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis Gene:KRR1(YCL059C)|FD-Score:-3.19|P-value:7.09E-4|Clearance:0||SGD DESC:Essential nucleolar protein required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit Gene:NUP82(YJL061W)|FD-Score:3.23|P-value:6.24E-4|Clearance:0.11||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p) Gene:ORC2(YBR060C)|FD-Score:-4.49|P-value:3.63E-6|Clearance:0||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; interacts with Spp1p and with trimethylated histone H3; phosphorylated by Cdc28p Gene:OST2(YOR103C)|FD-Score:-4.86|P-value:5.88E-7|Clearance:0||SGD DESC:Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:POL2(YNL262W)|FD-Score:4.42|P-value:4.95E-6|Clearance:0.21||SGD DESC:Catalytic subunit of DNA polymerase (II) epsilon, a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p Gene:PRP11(YDL043C)|FD-Score:-3.3|P-value:4.90E-4|Clearance:0||SGD DESC:Subunit of the SF3a splicing factor complex, required for spliceosome assembly Gene:RET2(YFR051C)|FD-Score:3.54|P-value:1.97E-4|Clearance:0.14||SGD DESC:Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RPB7(YDR404C)|FD-Score:3.91|P-value:4.62E-5|Clearance:0.18||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPC17(YJL011C)|FD-Score:3.66|P-value:1.24E-4|Clearance:0.01||SGD DESC:RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress Gene:SCD5(YOR329C)|FD-Score:5.31|P-value:5.49E-8|Clearance:0.67||SGD DESC:Protein required for normal actin organization and endocytosis; targeting subunit for protein phosphatase type 1; undergoes Crm1p-dependent nuclear-cytoplasmic shuttling; multicopy suppressor of clathrin deficiency Gene:SEC17(YBL050W)|FD-Score:3.28|P-value:5.13E-4|Clearance:0.06||SGD DESC:Peripheral membrane protein required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, and autophagy; stimulates the ATPase activity of Sec18p; has similarity to mammalian alpha-SNAP Gene:SMC4(YLR086W)|FD-Score:3.59|P-value:1.68E-4|Clearance:0.04||SGD DESC:Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate Gene:SMT3(YDR510W)|FD-Score:3.73|P-value:9.52E-5|Clearance:0.03||SGD DESC:Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2 Gene:SNU13(YEL026W)|FD-Score:3.7|P-value:1.07E-4|Clearance:0.04||SGD DESC:RNA binding protein, part of U3 snoRNP involved in rRNA processing, part of U4/U6-U5 tri-snRNP involved in mRNA splicing, similar to human 15.5K protein Gene:TSR4(YOL022C)|FD-Score:3.59|P-value:1.63E-4|Clearance:0.01||SGD DESC:Cytoplasmic protein required for correct processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; essential gene in S288C background but not in CEN.PK2 Gene:UTP15(YMR093W)|FD-Score:3.11|P-value:9.25E-4|Clearance:0.07||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YGR190C(YGR190C_d)|FD-Score:-3.27|P-value:5.35E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W Gene:YHC1(YLR298C)|FD-Score:-3.14|P-value:8.32E-4|Clearance:0||SGD DESC:Component of the U1 snRNP complex required for pre-mRNA splicing; putative ortholog of human U1C protein, which is involved in formation of a complex between U1 snRNP and the pre-mRNA 5' splice site Gene:YKL083W(YKL083W_d)|FD-Score:4.21|P-value:1.28E-5|Clearance:0.3||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene RRP14 Gene:YOR146W(YOR146W_d)|FD-Score:3.41|P-value:3.29E-4|Clearance:0.12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2970879
Download HOP data (tab-delimited text)  (excel)
Gene:ADE17(YMR120C)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE17 has a paralog, ADE16, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine Gene:AIM5(YBR262C)|FD-Score:5.39|P-value:3.55E-8||SGD DESC:Mitochondrial inner membrane protein; subunit of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:ARP5(YNL059C)|FD-Score:3.98|P-value:3.44E-5||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:ATE1(YGL017W)|FD-Score:-3.17|P-value:7.52E-4||SGD DESC:Arginyl-tRNA-protein transferase, catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway Gene:ATP2(YJR121W)|FD-Score:3.94|P-value:4.01E-5||SGD DESC:Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:BMH2(YDR099W)|FD-Score:-3.09|P-value:9.94E-4||SGD DESC:14-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:CAF40(YNL288W)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Evolutionarily conserved subunit of the CCR4-NOT complex involved in controlling mRNA initiation, elongation and degradation; binds Cdc39p Gene:CHL4(YDR254W)|FD-Score:-3.64|P-value:1.34E-4||SGD DESC:Outer kinetochore protein required for chromosome stability; involved in new kinetochore assembly and sister chromatid cohesion; peripheral component of the Ctf19 kinetochore complex that interacts with Ctf19p, Ctf3p, Iml3p and Mif2p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-N and fission yeast mis15 Gene:CHO1(YER026C)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:Phosphatidylserine synthase, functions in phospholipid biosynthesis; catalyzes the reaction CDP-diaclyglycerol + L-serine = CMP + L-1-phosphatidylserine, transcriptionally repressed by myo-inositol and choline Gene:CHS6(YJL099W)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication Gene:COQ10(YOL008W)|FD-Score:-3.77|P-value:8.24E-5||SGD DESC:Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q<sub>6</sub> to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes Gene:CPA2(YJR109C)|FD-Score:-3.67|P-value:1.20E-4||SGD DESC:Large subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor Gene:DAL3(YIR032C)|FD-Score:-3.19|P-value:7.16E-4||SGD DESC:Ureidoglycolate hydrolase, converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:DEF1(YKL054C)|FD-Score:3.19|P-value:7.11E-4||SGD DESC:RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and proteolysis of RNAPII present in an elongation complex; mutant is deficient in Zip1p loading onto chromosomes during meiosis Gene:EEB1(YPL095C)|FD-Score:4.24|P-value:1.14E-5||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short-chain esterase activity; may be involved in lipid metabolism and detoxification; EEB1 has a paralog, EHT1, that arose from the whole genome duplication Gene:EKI1(YDR147W)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Ethanolamine kinase; primarily responsible for phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; exhibits some choline kinase activity, thus contributing to phosphatidylcholine synthesis via the CDP-choline pathway; EKI1 has a paralog, CKI1, that arose from the whole genome duplication Gene:EMC4(YGL231C)|FD-Score:-4.09|P-value:2.20E-5||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4, fly CG11137, human TMM85 Gene:EMI1(YDR512C)|FD-Score:-3.77|P-value:8.32E-5||SGD DESC:Non-essential protein required for transcriptional induction of the early meiotic-specific transcription factor IME1, also required for sporulation; contains twin cysteine-x9-cysteine motifs Gene:FBP26(YJL155C)|FD-Score:5.92|P-value:1.62E-9||SGD DESC:Fructose-2,6-bisphosphatase, required for glucose metabolism; protein abundance increases in response to DNA replication stress Gene:FMP52(YER004W)|FD-Score:-3.85|P-value:5.81E-5||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:GET2(YER083C)|FD-Score:3.13|P-value:8.66E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GGA2(YHR108W)|FD-Score:7.56|P-value:2.00E-14||SGD DESC:Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin Gene:GPM2(YDL021W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:GUD1(YDL238C)|FD-Score:-3.42|P-value:3.14E-4||SGD DESC:Guanine deaminase, a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures Gene:HNT3(YOR258W)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:HSL7(YBR133C)|FD-Score:3.23|P-value:6.29E-4||SGD DESC:Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress Gene:IGO2(YHR132W-A)|FD-Score:-6.03|P-value:8.42E-10||SGD DESC:Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; similar to Igo1p Gene:INM2(YDR287W)|FD-Score:-3.35|P-value:4.08E-4||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol; enzymatic activity requires magnesium ions and is inhibited by lithium and sodium ions; inm1 inm2 double mutant lacks inositol auxotrophy Gene:IRC6(YFR043C)|FD-Score:6.72|P-value:9.00E-12||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:KAR4(YCL055W)|FD-Score:3.84|P-value:6.27E-5||SGD DESC:Transcription factor required for gene regulation in response to pheromones; also required during meiosis; exists in two forms, a slower-migrating form more abundant during vegetative growth and a faster-migrating form induced by pheromone Gene:KES1(YPL145C)|FD-Score:5.32|P-value:5.06E-8||SGD DESC:One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication Gene:LAS21(YJL062W)|FD-Score:3.22|P-value:6.51E-4||SGD DESC:Integral plasma membrane protein involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity Gene:MAD2(YJL030W)|FD-Score:3.28|P-value:5.25E-4||SGD DESC:Component of the spindle-assembly checkpoint complex; delays the onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p; regulates APC/C activity during prometaphase and metaphase of meiosis I Gene:MEF1(YLR069C)|FD-Score:3.94|P-value:4.10E-5||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MTC6(YHR151C)|FD-Score:3.86|P-value:5.62E-5||SGD DESC:Protein of unknown function; mtc6 is synthetically sick with cdc13-1 Gene:MTL1(YGR023W)|FD-Score:-3.25|P-value:5.82E-4||SGD DESC:Putative plasma membrane sensor, involved in cell integrity signaling and stress response during glucose starvation and oxidative stress; has structural and functional similarity to Mid2p Gene:PEP7(YDR323C)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PET494(YNR045W)|FD-Score:3.12|P-value:8.96E-4||SGD DESC:Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet122p; located in the mitochondrial inner membrane Gene:PEX18(YHR160C)|FD-Score:3.34|P-value:4.20E-4||SGD DESC:Peroxin required for targeting of peroxisomal matrix proteins containing PTS2; interacts with Pex7p; partially redundant with Pex21p Gene:PHO23(YNL097C)|FD-Score:-3.24|P-value:6.01E-4||SGD DESC:Probable component of the Rpd3 histone deacetylase complex, involved in transcriptional regulation of PHO5; affects termination of snoRNAs and cryptic unstable transcripts (CUTs); C-terminus has similarity to human candidate tumor suppressor p33(ING1) and its isoform ING3 Gene:PLM2(YDR501W)|FD-Score:3.74|P-value:9.30E-5||SGD DESC:Putative transcription factor, contains Forkhead Associated domain; found associated with chromatin; target of SBF transcription factor; induced in response to DNA damaging agents and deletion of telomerase; PLM2 has a paralog, TOS4, that arose from the whole genome duplication Gene:PRS1(YKL181W)|FD-Score:9.59|P-value:4.48E-22||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:PSH1(YOL054W)|FD-Score:3.88|P-value:5.26E-5||SGD DESC:E3 ubiquitin ligase that mediates poyubiquitination and degradation of centromere-binding protein Cse4p and prevents Cse4p from mislocalizing to euchromatin; ubiquitylation of Cse4p may be antagonized by Scm3p Gene:PYC1(YGL062W)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc2p; mutations in the human homolog are associated with lactic acidosis; PYC1 has a paralog, PYC2, that arose from the whole genome duplication Gene:RAV2(YDR202C)|FD-Score:-4.97|P-value:3.31E-7||SGD DESC:Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme Gene:RAX2(YLR084C)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:N-glycosylated protein involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; RAX2 mRNA stability is regulated by Mpt5p Gene:RIC1(YLR039C)|FD-Score:3.98|P-value:3.45E-5||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RNH1(YMR234W)|FD-Score:5.5|P-value:1.94E-8||SGD DESC:Ribonuclease H1; able to bind double-stranded RNAs and RNA-DNA hybrids; associates with RNAse polymerase I; the homolog of mammalian RNAse HII (the S. cerevisiae homolog of mammalian RNAse HI is RNH201) Gene:RNH70(YGR276C)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:3'-5' exoribonuclease; required for maturation of 3' ends of 5S rRNA and tRNA-Arg3 from dicistronic transcripts Gene:RPL31A(YDL075W)|FD-Score:-4.88|P-value:5.19E-7||SGD DESC:Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication Gene:RPL34A(YER056C-A)|FD-Score:3.77|P-value:8.24E-5||SGD DESC:Ribosomal 60S subunit protein L34A; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34A has a paralog, RPL34B, that arose from the whole genome duplication Gene:RPL6A(YML073C)|FD-Score:3.37|P-value:3.69E-4||SGD DESC:Ribosomal 60S subunit protein L6A; N-terminally acetylated; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6A has a paralog, RPL6B, that arose from the whole genome duplication Gene:RRI2(YOL117W)|FD-Score:3.23|P-value:6.24E-4||SGD DESC:Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response Gene:RSC2(YLR357W)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; involved in telomere maintenance Gene:RSM7(YJR113C)|FD-Score:6.11|P-value:5.08E-10||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein Gene:RTC2(YBR147W)|FD-Score:3.26|P-value:5.56E-4||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; positive regulation by Lys14p suggests that lysine may be the primary substrate; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter Gene:RUD3(YOR216C)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:Golgi matrix protein involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1 Gene:SAM2(YDR502C)|FD-Score:-3.88|P-value:5.27E-5||SGD DESC:S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM2 has a paralog, SAM1, that arose from the whole genome duplication Gene:SGM1(YJR134C)|FD-Score:-4.83|P-value:6.90E-7||SGD DESC:Protein of unknown function, required for wild-type growth rate on galactose and mannose; localizes to COPI coated vesicles and the Golgi apparatus Gene:SLM6(YBR266C_d)|FD-Score:5.8|P-value:3.36E-9||SGD DESC:Protein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase Gene:SNC1(YAL030W)|FD-Score:3.16|P-value:7.83E-4||SGD DESC:Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; proposed to be involved in endocytosis; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC1 has a paralog, SNC2, that arose from the whole genome duplication Gene:SNU66(YOR308C)|FD-Score:-4.02|P-value:2.85E-5||SGD DESC:Component of the U4/U6.U5 snRNP complex involved in pre-mRNA splicing via spliceosome; also required for pre-5S rRNA processing and may act in concert with Rnh70p; has homology to human SART-1 Gene:SPE4(YLR146C)|FD-Score:4.21|P-value:1.29E-5||SGD DESC:Spermine synthase, required for the biosynthesis of spermine and also involved in biosynthesis of pantothenic acid Gene:SSE1(YPL106C)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:ATPase component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSE1 has a paralog, SSE2, that arose from the whole genome duplication Gene:STO1(YMR125W)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:SYF2(YGR129W)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:TDA8(YAL064C-A_p)|FD-Score:3.85|P-value:5.83E-5||SGD DESC:Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene Gene:TRS33(YOR115C)|FD-Score:5.81|P-value:3.21E-9||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:UPF3(YGR072W)|FD-Score:4.28|P-value:9.29E-6||SGD DESC:Component of the nonsense-mediated mRNA decay (NMD) pathway, along with Nam7p and Nmd2p; involved in decay of mRNA containing nonsense codons; involved in telomere maintenance Gene:VCX1(YDL128W)|FD-Score:3.23|P-value:6.10E-4||SGD DESC:Vacuolar membrane antiporter with Ca2+/H+ and K+/H+ exchange activity, involved in control of cytosolic Ca2+ and K+ concentrations; has similarity to sodium/calcium exchangers, including the bovine Na+/Ca2+,K+ antiporter Gene:VMR1(YHL035C)|FD-Score:-3.48|P-value:2.47E-4||SGD DESC:Vacuolar membrane protein involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions Gene:VOA1(YGR106C)|FD-Score:5.3|P-value:5.85E-8||SGD DESC:Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval Gene:YAR047C(YAR047C_d)|FD-Score:3.22|P-value:6.47E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBL044W(YBL044W_p)|FD-Score:-4.71|P-value:1.26E-6||SGD DESC:Putative protein of unknown function; YBL044W is not an essential protein Gene:YBP2(YGL060W)|FD-Score:6.57|P-value:2.46E-11||SGD DESC:Central kinetochore associated protein; mediates mitotic progression; interacts with several central kinetochore proteins and centromeric histone Cse4p; role in resistance to oxidative stress; similar to Slk19p; YBP2 has a paralog, YBP1, that arose from the whole genome duplication Gene:YCL076W(YCL076W_d)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL071C(YDL071C_d)|FD-Score:-3.4|P-value:3.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF BDF2/YDL070W Gene:YDR029W(YDR029W_d)|FD-Score:-3.33|P-value:4.40E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR131C(YDR131C_p)|FD-Score:4.08|P-value:2.25E-5||SGD DESC:F-box protein, substrate-specific adaptor subunit that recruits substrates to a core ubiquitination complex Gene:YEL043W(YEL043W)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Predicted cytoskeleton protein involved in intracellular signalling based on quantitative analysis of protein-protein interaction maps; may interact with ribosomes, based on co-purification studies; contains fibronectin type III domain fold Gene:YEL1(YBL060W)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Guanine nucleotide exchange factor specific for Arf3p; localized to the bud neck and tip; required for localization of Arf3p to the bud neck and tip Gene:YER079W(YER079W_p)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Putative protein of unknown function Gene:YFR016C(YFR016C)|FD-Score:3.81|P-value:6.88E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and bud; interacts with Spa2p; YFL016C is not an essential gene Gene:YGL132W(YGL132W_d)|FD-Score:3.27|P-value:5.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SNT2/YGL131C Gene:YIL055C(YIL055C_p)|FD-Score:3.75|P-value:8.85E-5||SGD DESC:Putative protein of unknown function Gene:YJL150W(YJL150W_d)|FD-Score:3.12|P-value:9.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKR011C(YKR011C_p)|FD-Score:3.46|P-value:2.73E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; protein abundance increases in response to DNA replication stress Gene:YKR104W(YKR104W)|FD-Score:-3.71|P-value:1.04E-4||SGD DESC:Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; contains a stop codon in S288C; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds Gene:YLF2(YHL014C)|FD-Score:3.25|P-value:5.73E-4||SGD DESC:Protein of unknown function, has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YMR124W(YMR124W_p)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization Gene:YMR310C(YMR310C_p)|FD-Score:-4.28|P-value:9.54E-6||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR310C is not an essential gene Gene:YNL277W-A(YNL277W-A_p)|FD-Score:5.42|P-value:2.97E-8||SGD DESC:Putative protein of unknown function Gene:YNR064C(YNR064C)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:Epoxide hydrolase, member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides Gene:YOR316C-A(YOR316C-A_p)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YOR331C(YOR331C_d)|FD-Score:-3.94|P-value:4.12E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene VMA4/YOR332W Gene:YPL109C(YPL109C_p)|FD-Score:-3.59|P-value:1.66E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPR059C(YPR059C_d)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YMC1/YPR058W Gene:YPR127W(YPR127W)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:Putative pyridoxine 4-dehydrogenase; differentially expressed during alcoholic fermentation; expression activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YTA6(YPL074W)|FD-Score:-3.41|P-value:3.29E-4||SGD DESC:Putative ATPase of the CDC48/PAS1/SEC18 (AAA) family; localized to the cortex of mother cells but not to daughter cells; relocalizes from cytoplasm to plasma membrane foci upon DNA replication stress Gene:ZRG17(YNR039C)|FD-Score:3.94|P-value:4.07E-5||SGD DESC:Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Msc2p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; zinc-regulated directly through Zap1p; transcription induced under conditions of zinc deficiency Gene:ADE17(YMR120C)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE17 has a paralog, ADE16, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine Gene:AIM5(YBR262C)|FD-Score:5.39|P-value:3.55E-8||SGD DESC:Mitochondrial inner membrane protein; subunit of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:ARP5(YNL059C)|FD-Score:3.98|P-value:3.44E-5||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:ATE1(YGL017W)|FD-Score:-3.17|P-value:7.52E-4||SGD DESC:Arginyl-tRNA-protein transferase, catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway Gene:ATP2(YJR121W)|FD-Score:3.94|P-value:4.01E-5||SGD DESC:Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:BMH2(YDR099W)|FD-Score:-3.09|P-value:9.94E-4||SGD DESC:14-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:CAF40(YNL288W)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Evolutionarily conserved subunit of the CCR4-NOT complex involved in controlling mRNA initiation, elongation and degradation; binds Cdc39p Gene:CHL4(YDR254W)|FD-Score:-3.64|P-value:1.34E-4||SGD DESC:Outer kinetochore protein required for chromosome stability; involved in new kinetochore assembly and sister chromatid cohesion; peripheral component of the Ctf19 kinetochore complex that interacts with Ctf19p, Ctf3p, Iml3p and Mif2p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-N and fission yeast mis15 Gene:CHO1(YER026C)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:Phosphatidylserine synthase, functions in phospholipid biosynthesis; catalyzes the reaction CDP-diaclyglycerol + L-serine = CMP + L-1-phosphatidylserine, transcriptionally repressed by myo-inositol and choline Gene:CHS6(YJL099W)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication Gene:COQ10(YOL008W)|FD-Score:-3.77|P-value:8.24E-5||SGD DESC:Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q<sub>6</sub> to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes Gene:CPA2(YJR109C)|FD-Score:-3.67|P-value:1.20E-4||SGD DESC:Large subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor Gene:DAL3(YIR032C)|FD-Score:-3.19|P-value:7.16E-4||SGD DESC:Ureidoglycolate hydrolase, converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:DEF1(YKL054C)|FD-Score:3.19|P-value:7.11E-4||SGD DESC:RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and proteolysis of RNAPII present in an elongation complex; mutant is deficient in Zip1p loading onto chromosomes during meiosis Gene:EEB1(YPL095C)|FD-Score:4.24|P-value:1.14E-5||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short-chain esterase activity; may be involved in lipid metabolism and detoxification; EEB1 has a paralog, EHT1, that arose from the whole genome duplication Gene:EKI1(YDR147W)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Ethanolamine kinase; primarily responsible for phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; exhibits some choline kinase activity, thus contributing to phosphatidylcholine synthesis via the CDP-choline pathway; EKI1 has a paralog, CKI1, that arose from the whole genome duplication Gene:EMC4(YGL231C)|FD-Score:-4.09|P-value:2.20E-5||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4, fly CG11137, human TMM85 Gene:EMI1(YDR512C)|FD-Score:-3.77|P-value:8.32E-5||SGD DESC:Non-essential protein required for transcriptional induction of the early meiotic-specific transcription factor IME1, also required for sporulation; contains twin cysteine-x9-cysteine motifs Gene:FBP26(YJL155C)|FD-Score:5.92|P-value:1.62E-9||SGD DESC:Fructose-2,6-bisphosphatase, required for glucose metabolism; protein abundance increases in response to DNA replication stress Gene:FMP52(YER004W)|FD-Score:-3.85|P-value:5.81E-5||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:GET2(YER083C)|FD-Score:3.13|P-value:8.66E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GGA2(YHR108W)|FD-Score:7.56|P-value:2.00E-14||SGD DESC:Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin Gene:GPM2(YDL021W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:GUD1(YDL238C)|FD-Score:-3.42|P-value:3.14E-4||SGD DESC:Guanine deaminase, a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures Gene:HNT3(YOR258W)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:HSL7(YBR133C)|FD-Score:3.23|P-value:6.29E-4||SGD DESC:Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress Gene:IGO2(YHR132W-A)|FD-Score:-6.03|P-value:8.42E-10||SGD DESC:Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; similar to Igo1p Gene:INM2(YDR287W)|FD-Score:-3.35|P-value:4.08E-4||SGD DESC:Inositol monophosphatase, involved in biosynthesis of inositol; enzymatic activity requires magnesium ions and is inhibited by lithium and sodium ions; inm1 inm2 double mutant lacks inositol auxotrophy Gene:IRC6(YFR043C)|FD-Score:6.72|P-value:9.00E-12||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:KAR4(YCL055W)|FD-Score:3.84|P-value:6.27E-5||SGD DESC:Transcription factor required for gene regulation in response to pheromones; also required during meiosis; exists in two forms, a slower-migrating form more abundant during vegetative growth and a faster-migrating form induced by pheromone Gene:KES1(YPL145C)|FD-Score:5.32|P-value:5.06E-8||SGD DESC:One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication Gene:LAS21(YJL062W)|FD-Score:3.22|P-value:6.51E-4||SGD DESC:Integral plasma membrane protein involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity Gene:MAD2(YJL030W)|FD-Score:3.28|P-value:5.25E-4||SGD DESC:Component of the spindle-assembly checkpoint complex; delays the onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p; regulates APC/C activity during prometaphase and metaphase of meiosis I Gene:MEF1(YLR069C)|FD-Score:3.94|P-value:4.10E-5||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MTC6(YHR151C)|FD-Score:3.86|P-value:5.62E-5||SGD DESC:Protein of unknown function; mtc6 is synthetically sick with cdc13-1 Gene:MTL1(YGR023W)|FD-Score:-3.25|P-value:5.82E-4||SGD DESC:Putative plasma membrane sensor, involved in cell integrity signaling and stress response during glucose starvation and oxidative stress; has structural and functional similarity to Mid2p Gene:PEP7(YDR323C)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PET494(YNR045W)|FD-Score:3.12|P-value:8.96E-4||SGD DESC:Mitochondrial translational activator specific for the COX3 mRNA, acts together with Pet54p and Pet122p; located in the mitochondrial inner membrane Gene:PEX18(YHR160C)|FD-Score:3.34|P-value:4.20E-4||SGD DESC:Peroxin required for targeting of peroxisomal matrix proteins containing PTS2; interacts with Pex7p; partially redundant with Pex21p Gene:PHO23(YNL097C)|FD-Score:-3.24|P-value:6.01E-4||SGD DESC:Probable component of the Rpd3 histone deacetylase complex, involved in transcriptional regulation of PHO5; affects termination of snoRNAs and cryptic unstable transcripts (CUTs); C-terminus has similarity to human candidate tumor suppressor p33(ING1) and its isoform ING3 Gene:PLM2(YDR501W)|FD-Score:3.74|P-value:9.30E-5||SGD DESC:Putative transcription factor, contains Forkhead Associated domain; found associated with chromatin; target of SBF transcription factor; induced in response to DNA damaging agents and deletion of telomerase; PLM2 has a paralog, TOS4, that arose from the whole genome duplication Gene:PRS1(YKL181W)|FD-Score:9.59|P-value:4.48E-22||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:PSH1(YOL054W)|FD-Score:3.88|P-value:5.26E-5||SGD DESC:E3 ubiquitin ligase that mediates poyubiquitination and degradation of centromere-binding protein Cse4p and prevents Cse4p from mislocalizing to euchromatin; ubiquitylation of Cse4p may be antagonized by Scm3p Gene:PYC1(YGL062W)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc2p; mutations in the human homolog are associated with lactic acidosis; PYC1 has a paralog, PYC2, that arose from the whole genome duplication Gene:RAV2(YDR202C)|FD-Score:-4.97|P-value:3.31E-7||SGD DESC:Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme Gene:RAX2(YLR084C)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:N-glycosylated protein involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; RAX2 mRNA stability is regulated by Mpt5p Gene:RIC1(YLR039C)|FD-Score:3.98|P-value:3.45E-5||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RNH1(YMR234W)|FD-Score:5.5|P-value:1.94E-8||SGD DESC:Ribonuclease H1; able to bind double-stranded RNAs and RNA-DNA hybrids; associates with RNAse polymerase I; the homolog of mammalian RNAse HII (the S. cerevisiae homolog of mammalian RNAse HI is RNH201) Gene:RNH70(YGR276C)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:3'-5' exoribonuclease; required for maturation of 3' ends of 5S rRNA and tRNA-Arg3 from dicistronic transcripts Gene:RPL31A(YDL075W)|FD-Score:-4.88|P-value:5.19E-7||SGD DESC:Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication Gene:RPL34A(YER056C-A)|FD-Score:3.77|P-value:8.24E-5||SGD DESC:Ribosomal 60S subunit protein L34A; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34A has a paralog, RPL34B, that arose from the whole genome duplication Gene:RPL6A(YML073C)|FD-Score:3.37|P-value:3.69E-4||SGD DESC:Ribosomal 60S subunit protein L6A; N-terminally acetylated; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6A has a paralog, RPL6B, that arose from the whole genome duplication Gene:RRI2(YOL117W)|FD-Score:3.23|P-value:6.24E-4||SGD DESC:Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response Gene:RSC2(YLR357W)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; involved in telomere maintenance Gene:RSM7(YJR113C)|FD-Score:6.11|P-value:5.08E-10||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein Gene:RTC2(YBR147W)|FD-Score:3.26|P-value:5.56E-4||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; positive regulation by Lys14p suggests that lysine may be the primary substrate; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter Gene:RUD3(YOR216C)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:Golgi matrix protein involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1 Gene:SAM2(YDR502C)|FD-Score:-3.88|P-value:5.27E-5||SGD DESC:S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM2 has a paralog, SAM1, that arose from the whole genome duplication Gene:SGM1(YJR134C)|FD-Score:-4.83|P-value:6.90E-7||SGD DESC:Protein of unknown function, required for wild-type growth rate on galactose and mannose; localizes to COPI coated vesicles and the Golgi apparatus Gene:SLM6(YBR266C_d)|FD-Score:5.8|P-value:3.36E-9||SGD DESC:Protein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase Gene:SNC1(YAL030W)|FD-Score:3.16|P-value:7.83E-4||SGD DESC:Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; proposed to be involved in endocytosis; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC1 has a paralog, SNC2, that arose from the whole genome duplication Gene:SNU66(YOR308C)|FD-Score:-4.02|P-value:2.85E-5||SGD DESC:Component of the U4/U6.U5 snRNP complex involved in pre-mRNA splicing via spliceosome; also required for pre-5S rRNA processing and may act in concert with Rnh70p; has homology to human SART-1 Gene:SPE4(YLR146C)|FD-Score:4.21|P-value:1.29E-5||SGD DESC:Spermine synthase, required for the biosynthesis of spermine and also involved in biosynthesis of pantothenic acid Gene:SSE1(YPL106C)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:ATPase component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSE1 has a paralog, SSE2, that arose from the whole genome duplication Gene:STO1(YMR125W)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:SYF2(YGR129W)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:TDA8(YAL064C-A_p)|FD-Score:3.85|P-value:5.83E-5||SGD DESC:Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene Gene:TRS33(YOR115C)|FD-Score:5.81|P-value:3.21E-9||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:UPF3(YGR072W)|FD-Score:4.28|P-value:9.29E-6||SGD DESC:Component of the nonsense-mediated mRNA decay (NMD) pathway, along with Nam7p and Nmd2p; involved in decay of mRNA containing nonsense codons; involved in telomere maintenance Gene:VCX1(YDL128W)|FD-Score:3.23|P-value:6.10E-4||SGD DESC:Vacuolar membrane antiporter with Ca2+/H+ and K+/H+ exchange activity, involved in control of cytosolic Ca2+ and K+ concentrations; has similarity to sodium/calcium exchangers, including the bovine Na+/Ca2+,K+ antiporter Gene:VMR1(YHL035C)|FD-Score:-3.48|P-value:2.47E-4||SGD DESC:Vacuolar membrane protein involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions Gene:VOA1(YGR106C)|FD-Score:5.3|P-value:5.85E-8||SGD DESC:Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval Gene:YAR047C(YAR047C_d)|FD-Score:3.22|P-value:6.47E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBL044W(YBL044W_p)|FD-Score:-4.71|P-value:1.26E-6||SGD DESC:Putative protein of unknown function; YBL044W is not an essential protein Gene:YBP2(YGL060W)|FD-Score:6.57|P-value:2.46E-11||SGD DESC:Central kinetochore associated protein; mediates mitotic progression; interacts with several central kinetochore proteins and centromeric histone Cse4p; role in resistance to oxidative stress; similar to Slk19p; YBP2 has a paralog, YBP1, that arose from the whole genome duplication Gene:YCL076W(YCL076W_d)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL071C(YDL071C_d)|FD-Score:-3.4|P-value:3.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF BDF2/YDL070W Gene:YDR029W(YDR029W_d)|FD-Score:-3.33|P-value:4.40E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR131C(YDR131C_p)|FD-Score:4.08|P-value:2.25E-5||SGD DESC:F-box protein, substrate-specific adaptor subunit that recruits substrates to a core ubiquitination complex Gene:YEL043W(YEL043W)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Predicted cytoskeleton protein involved in intracellular signalling based on quantitative analysis of protein-protein interaction maps; may interact with ribosomes, based on co-purification studies; contains fibronectin type III domain fold Gene:YEL1(YBL060W)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Guanine nucleotide exchange factor specific for Arf3p; localized to the bud neck and tip; required for localization of Arf3p to the bud neck and tip Gene:YER079W(YER079W_p)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Putative protein of unknown function Gene:YFR016C(YFR016C)|FD-Score:3.81|P-value:6.88E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and bud; interacts with Spa2p; YFL016C is not an essential gene Gene:YGL132W(YGL132W_d)|FD-Score:3.27|P-value:5.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SNT2/YGL131C Gene:YIL055C(YIL055C_p)|FD-Score:3.75|P-value:8.85E-5||SGD DESC:Putative protein of unknown function Gene:YJL150W(YJL150W_d)|FD-Score:3.12|P-value:9.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKR011C(YKR011C_p)|FD-Score:3.46|P-value:2.73E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; protein abundance increases in response to DNA replication stress Gene:YKR104W(YKR104W)|FD-Score:-3.71|P-value:1.04E-4||SGD DESC:Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; contains a stop codon in S288C; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds Gene:YLF2(YHL014C)|FD-Score:3.25|P-value:5.73E-4||SGD DESC:Protein of unknown function, has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YMR124W(YMR124W_p)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization Gene:YMR310C(YMR310C_p)|FD-Score:-4.28|P-value:9.54E-6||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR310C is not an essential gene Gene:YNL277W-A(YNL277W-A_p)|FD-Score:5.42|P-value:2.97E-8||SGD DESC:Putative protein of unknown function Gene:YNR064C(YNR064C)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:Epoxide hydrolase, member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides Gene:YOR316C-A(YOR316C-A_p)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YOR331C(YOR331C_d)|FD-Score:-3.94|P-value:4.12E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene VMA4/YOR332W Gene:YPL109C(YPL109C_p)|FD-Score:-3.59|P-value:1.66E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPR059C(YPR059C_d)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YMC1/YPR058W Gene:YPR127W(YPR127W)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:Putative pyridoxine 4-dehydrogenase; differentially expressed during alcoholic fermentation; expression activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YTA6(YPL074W)|FD-Score:-3.41|P-value:3.29E-4||SGD DESC:Putative ATPase of the CDC48/PAS1/SEC18 (AAA) family; localized to the cortex of mother cells but not to daughter cells; relocalizes from cytoplasm to plasma membrane foci upon DNA replication stress Gene:ZRG17(YNR039C)|FD-Score:3.94|P-value:4.07E-5||SGD DESC:Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Msc2p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; zinc-regulated directly through Zap1p; transcription induced under conditions of zinc deficiency

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR329C5.315.49E-80.67SCD5Protein required for normal actin organization and endocytosis; targeting subunit for protein phosphatase type 1; undergoes Crm1p-dependent nuclear-cytoplasmic shuttling; multicopy suppressor of clathrin deficiency
YNL118C4.641.74E-60.22DCP2Catalytic subunit of the Dcp1p-Dcp2p decapping enzyme complex; removes the 5' cap structure from mRNAs prior to their degradation; nudix hydrolase family member; forms cytoplasmic foci upon DNA replication stress
YNL262W4.424.95E-60.21POL2Catalytic subunit of DNA polymerase (II) epsilon, a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p
YKL083W_d4.211.28E-50.30YKL083W_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene RRP14
YDR404C3.914.62E-50.18RPB7RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation
YDR510W3.739.52E-50.03SMT3Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2
YEL026W3.701.07E-40.04SNU13RNA binding protein, part of U3 snoRNP involved in rRNA processing, part of U4/U6-U5 tri-snRNP involved in mRNA splicing, similar to human 15.5K protein
YJL011C3.661.24E-40.01RPC17RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress
YOR260W3.661.28E-40.02GCD1Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YGR029W3.641.38E-40.04ERV1Flavin-linked sulfhydryl oxidase of the mitochondrial intermembrane space (IMS), oxidizes Mia40p as part of a disulfide relay system that promotes IMS retention of imported proteins; ortholog of human hepatopoietin (ALR)
YOL022C3.591.63E-40.01TSR4Cytoplasmic protein required for correct processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; essential gene in S288C background but not in CEN.PK2
YLR086W3.591.68E-40.04SMC4Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate
YFR051C3.541.97E-40.14RET2Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER
YDR299W3.413.28E-46.30E-4BFR2Essential protein that is a component of 90S preribosomes; may be involved in rRNA processing; multicopy suppressor of sensitivity to Brefeldin A; expression is induced during lag phase and also by cold shock
YOR146W_d3.413.29E-40.12YOR146W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKL181W9.594.48E-22PRS15-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes
YHR108W7.562.00E-14GGA2Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin
YFR043C6.729.00E-12IRC6Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci
YGL060W6.572.46E-11YBP2Central kinetochore associated protein; mediates mitotic progression; interacts with several central kinetochore proteins and centromeric histone Cse4p; role in resistance to oxidative stress; similar to Slk19p; YBP2 has a paralog, YBP1, that arose from the whole genome duplication
YJR113C6.115.08E-10RSM7Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein
YJL155C5.921.62E-9FBP26Fructose-2,6-bisphosphatase, required for glucose metabolism; protein abundance increases in response to DNA replication stress
YOR115C5.813.21E-9TRS33One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic
YBR266C_d5.803.36E-9SLM6_dProtein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase
YMR234W5.501.94E-8RNH1Ribonuclease H1; able to bind double-stranded RNAs and RNA-DNA hybrids; associates with RNAse polymerase I; the homolog of mammalian RNAse HII (the S. cerevisiae homolog of mammalian RNAse HI is RNH201)
YNL277W-A_p5.422.97E-8YNL277W-A_pPutative protein of unknown function
YBR262C5.393.55E-8AIM5Mitochondrial inner membrane protein; subunit of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture
YPL145C5.325.06E-8KES1One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication
YGR106C5.305.85E-8VOA1Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval
YGR072W4.289.29E-6UPF3Component of the nonsense-mediated mRNA decay (NMD) pathway, along with Nam7p and Nmd2p; involved in decay of mRNA containing nonsense codons; involved in telomere maintenance
YBL060W4.241.12E-5YEL1Guanine nucleotide exchange factor specific for Arf3p; localized to the bud neck and tip; required for localization of Arf3p to the bud neck and tip

GO enrichment analysis for SGTC_2808
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0756.78E-9SGTC_28077945238 52.0 μMChembridge (Drug-like library)171487970.209877
0.0691.29E-7SGTC_28067952793 58.4 μMChembridge (Drug-like library)29724900.0481928
0.0631.25E-6SGTC_24415730946 39.1 μMMiscellaneous17742750.0947368
0.0612.31E-6SGTC_21455634607 200.0 μMChembridge (Fragment library)3086620.0789474
0.0587.36E-6SGTC_21355326629 150.4 μMChembridge (Fragment library)50200770.0869565RPP1 & pyrimidine depletion
0.0543.42E-5SGTC_28047954240 19.5 μMChembridge (Drug-like library)8287050.0652174
0.0534.10E-5SGTC_33219137793 26.8 μMChembridge (Drug-like library)49041920.0526316cell wall
0.0534.81E-5SGTC_1762st045503 25.5 μMTimTec (Natural product derivative library)46455540.0721649
0.0525.96E-5SGTC_9164466-0038 42.0 nMChemDiv (Drug-like library)57220640.0641026cell wall
0.0501.36E-4SGTC_33119131146 2.9 μMChembridge (Drug-like library)49004060.0537634cell wall
0.0491.65E-4SGTC_1760st045453 10.4 μMTimTec (Natural product derivative library)33234190.0786517NEO1-PIK1
0.0491.69E-4SGTC_20875276107 200.0 μMChembridge (Fragment library)1444050.125
0.0491.82E-4SGTC_1871488-2104 32.7 μMChemDiv (Drug-like library)28528590.0918367calcium & mitochondrial duress
0.0491.92E-4SGTC_30899117425 49.5 μMChembridge (Drug-like library)94016570.141026
0.0482.12E-4SGTC_22847945075 39.2 μMChembridge (Fragment library)12454020.171053mitochondrial processes

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3145908551649.47 μM0.44262325236195Chembridge (Drug-like library)306.466143.79903fatty acid desaturase (OLE1)
SGTC_22647948757110.76 μM0.3442622971411Chembridge (Fragment library)252.312721.35624
SGTC_3216912971649.47 μM0.3285718056934Chembridge (Drug-like library)310.368721.40815RPP1 & pyrimidine depletion
SGTC_22637948595193.29 μM0.3076922971371Chembridge (Fragment library)236.247120.79824
SGTC_2274794709446.08 μM0.3064522971028Chembridge (Fragment library)246.2851.40323
SGTC_2170morpholine200 μM0.292308121171Chembridge (Fragment library)234.294221.09303
SGTC_9133487-1245167 μM0.2916674016153ChemDiv (Drug-like library)349.831844.53424
SGTC_2992907459171.43 μM0.28378417741321Chembridge (Drug-like library)374.453981.91215
SGTC_22857945142200 μM0.2794122970608Chembridge (Fragment library)247.273060.25224
SGTC_2276794751342.08 μM0.2769232105100Chembridge (Fragment library)234.297442.23314