7967822

N-[2-methoxy-4-[(2,3,5,6-tetrafluoro-4-methoxybenzoyl)amino]phenyl]thiophene-2-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2811
Screen concentration 32.5 μM
Source Chembridge (Drug-like library)
PubChem CID 2232244
SMILES COC1=C(C=CC(=C1)NC(=O)C2=C(C(=C(C(=C2F)F)OC)F)F)NC(=O)C3=CC=CS3
Standardized SMILES COc1cc(NC(=O)c2c(F)c(F)c(OC)c(F)c2F)ccc1NC(=O)c3cccs3
Molecular weight 454.3948
ALogP 4.14
H-bond donor count 2
H-bond acceptor count 9
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.27
% growth inhibition (Hom. pool) 3.82


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2232244
Download HIP data (tab-delimited text)  (excel)
Gene:CAK1(YFL029C)|FD-Score:-3.65|P-value:1.29E-4|Clearance:0||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:CDC12(YHR107C)|FD-Score:3.86|P-value:5.57E-5|Clearance:0.1||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:COF1(YLL050C)|FD-Score:-3.11|P-value:9.45E-4|Clearance:0||SGD DESC:Cofilin, involved in pH-dependent actin filament depolarization; binds both actin monomers and filaments and severs filaments; involved in the selective sorting, export of the secretory cargo from the late golgi; genetically interacts with pmr1; thought to be regulated by phosphorylation at SER4; ubiquitous and essential in eukaryotes Gene:CSL4(YNL232W)|FD-Score:-3.7|P-value:1.07E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; has similarity to human hCsl4p (EXOSC1) Gene:ERG9(YHR190W)|FD-Score:4.91|P-value:4.67E-7|Clearance:0.5||SGD DESC:Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway Gene:HRR25(YPL204W)|FD-Score:3.38|P-value:3.58E-4|Clearance:0.06||SGD DESC:Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) Gene:KRS1(YDR037W)|FD-Score:-4.49|P-value:3.60E-6|Clearance:0||SGD DESC:Lysyl-tRNA synthetase Gene:LSG1(YGL099W)|FD-Score:4.68|P-value:1.46E-6|Clearance:0.5||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:LSM4(YER112W)|FD-Score:3.33|P-value:4.42E-4|Clearance:0.17||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:MPP10(YJR002W)|FD-Score:3.43|P-value:3.03E-4|Clearance:0.01||SGD DESC:Component of the SSU processome and 90S preribosome, required for pre-18S rRNA processing, interacts with and controls the stability of Imp3p and Imp4p, essential for viability; similar to human Mpp10p Gene:PDS1(YDR113C)|FD-Score:-5.88|P-value:2.09E-9|Clearance:0||SGD DESC:Securin, inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:POP8(YBL018C)|FD-Score:3.42|P-value:3.10E-4|Clearance:0.04||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PZF1(YPR186C)|FD-Score:3.11|P-value:9.44E-4|Clearance:0.04||SGD DESC:Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA Gene:RLP24(YLR009W)|FD-Score:3.68|P-value:1.19E-4|Clearance:0.18||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RVB2(YPL235W)|FD-Score:3.49|P-value:2.39E-4|Clearance:0.06||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SAH1(YER043C)|FD-Score:3.15|P-value:8.13E-4|Clearance:0.04||SGD DESC:S-adenosyl-L-homocysteine hydrolase, catabolizes S-adenosyl-L-homocysteine which is formed after donation of the activated methyl group of S-adenosyl-L-methionine (AdoMet) to an acceptor Gene:SEC13(YLR208W)|FD-Score:-4.12|P-value:1.86E-5|Clearance:0||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC20(YDR498C)|FD-Score:3.76|P-value:8.41E-5|Clearance:0.09||SGD DESC:Membrane glycoprotein v-SNARE involved in retrograde transport from the Golgi to the ER; required for N- and O-glycosylation in the Golgi but not in the ER; interacts with the Dsl1p complex through Tip20p Gene:SMC1(YFL008W)|FD-Score:-3.98|P-value:3.45E-5|Clearance:0||SGD DESC:Subunit of the multiprotein cohesin complex, essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure Gene:TIM22(YDL217C)|FD-Score:4.01|P-value:3.02E-5|Clearance:0.15||SGD DESC:Essential core component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported Gene:TRR1(YDR353W)|FD-Score:4.17|P-value:1.50E-5|Clearance:0.16||SGD DESC:Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication Gene:TSR4(YOL022C)|FD-Score:-5.32|P-value:5.29E-8|Clearance:0||SGD DESC:Cytoplasmic protein required for correct processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; essential gene in S288C background but not in CEN.PK2 Gene:USB1(YLR132C)|FD-Score:-4.43|P-value:4.74E-6|Clearance:0||SGD DESC:Putative phosphodiesterase specific for U6 snRNA 3' end modification; trims the 3' poly(u) tract to leave a terminal 3' phosphate; human homolog, hUSB1 (aka C16orf57) produces a 2',3' cyclic phosphate; mutations in hUSB1 are associated with a rare skin condition (OMIM 604173); essential protein that localizes to the nucleus and mitochondria; overexpression suppresses the respiratory defects of oxa1 and mtf2 mutants Gene:CAK1(YFL029C)|FD-Score:-3.65|P-value:1.29E-4|Clearance:0||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:CDC12(YHR107C)|FD-Score:3.86|P-value:5.57E-5|Clearance:0.1||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:COF1(YLL050C)|FD-Score:-3.11|P-value:9.45E-4|Clearance:0||SGD DESC:Cofilin, involved in pH-dependent actin filament depolarization; binds both actin monomers and filaments and severs filaments; involved in the selective sorting, export of the secretory cargo from the late golgi; genetically interacts with pmr1; thought to be regulated by phosphorylation at SER4; ubiquitous and essential in eukaryotes Gene:CSL4(YNL232W)|FD-Score:-3.7|P-value:1.07E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; has similarity to human hCsl4p (EXOSC1) Gene:ERG9(YHR190W)|FD-Score:4.91|P-value:4.67E-7|Clearance:0.5||SGD DESC:Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway Gene:HRR25(YPL204W)|FD-Score:3.38|P-value:3.58E-4|Clearance:0.06||SGD DESC:Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) Gene:KRS1(YDR037W)|FD-Score:-4.49|P-value:3.60E-6|Clearance:0||SGD DESC:Lysyl-tRNA synthetase Gene:LSG1(YGL099W)|FD-Score:4.68|P-value:1.46E-6|Clearance:0.5||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:LSM4(YER112W)|FD-Score:3.33|P-value:4.42E-4|Clearance:0.17||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:MPP10(YJR002W)|FD-Score:3.43|P-value:3.03E-4|Clearance:0.01||SGD DESC:Component of the SSU processome and 90S preribosome, required for pre-18S rRNA processing, interacts with and controls the stability of Imp3p and Imp4p, essential for viability; similar to human Mpp10p Gene:PDS1(YDR113C)|FD-Score:-5.88|P-value:2.09E-9|Clearance:0||SGD DESC:Securin, inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:POP8(YBL018C)|FD-Score:3.42|P-value:3.10E-4|Clearance:0.04||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PZF1(YPR186C)|FD-Score:3.11|P-value:9.44E-4|Clearance:0.04||SGD DESC:Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA Gene:RLP24(YLR009W)|FD-Score:3.68|P-value:1.19E-4|Clearance:0.18||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RVB2(YPL235W)|FD-Score:3.49|P-value:2.39E-4|Clearance:0.06||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SAH1(YER043C)|FD-Score:3.15|P-value:8.13E-4|Clearance:0.04||SGD DESC:S-adenosyl-L-homocysteine hydrolase, catabolizes S-adenosyl-L-homocysteine which is formed after donation of the activated methyl group of S-adenosyl-L-methionine (AdoMet) to an acceptor Gene:SEC13(YLR208W)|FD-Score:-4.12|P-value:1.86E-5|Clearance:0||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC20(YDR498C)|FD-Score:3.76|P-value:8.41E-5|Clearance:0.09||SGD DESC:Membrane glycoprotein v-SNARE involved in retrograde transport from the Golgi to the ER; required for N- and O-glycosylation in the Golgi but not in the ER; interacts with the Dsl1p complex through Tip20p Gene:SMC1(YFL008W)|FD-Score:-3.98|P-value:3.45E-5|Clearance:0||SGD DESC:Subunit of the multiprotein cohesin complex, essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure Gene:TIM22(YDL217C)|FD-Score:4.01|P-value:3.02E-5|Clearance:0.15||SGD DESC:Essential core component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported Gene:TRR1(YDR353W)|FD-Score:4.17|P-value:1.50E-5|Clearance:0.16||SGD DESC:Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication Gene:TSR4(YOL022C)|FD-Score:-5.32|P-value:5.29E-8|Clearance:0||SGD DESC:Cytoplasmic protein required for correct processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; essential gene in S288C background but not in CEN.PK2 Gene:USB1(YLR132C)|FD-Score:-4.43|P-value:4.74E-6|Clearance:0||SGD DESC:Putative phosphodiesterase specific for U6 snRNA 3' end modification; trims the 3' poly(u) tract to leave a terminal 3' phosphate; human homolog, hUSB1 (aka C16orf57) produces a 2',3' cyclic phosphate; mutations in hUSB1 are associated with a rare skin condition (OMIM 604173); essential protein that localizes to the nucleus and mitochondria; overexpression suppresses the respiratory defects of oxa1 and mtf2 mutants

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2232244
Download HOP data (tab-delimited text)  (excel)
Gene:ADR1(YDR216W)|FD-Score:3.14|P-value:8.34E-4||SGD DESC:Carbon source-responsive zinc-finger transcription factor, required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization Gene:AIM11(YER093C-A)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Protein of unknown function; null mutant is viable but shows increased loss of mitochondrial genome and synthetic interaction with prohibitin (phb1); contains an intron; YER093C-A has a paralog, YBL059W, that arose from the whole genome duplication Gene:ARO7(YPR060C)|FD-Score:3.23|P-value:6.15E-4||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:AST1(YBL069W)|FD-Score:3.72|P-value:9.95E-5||SGD DESC:Lipid raft associated protein; interacts with the plasma membrane ATPase Pma1p and has a role in its targeting to the plasma membrane by influencing its incorporation into lipid rafts; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST1 has a paralog, AST2, that arose from the whole genome duplication Gene:ATP15(YPL271W)|FD-Score:3.25|P-value:5.73E-4||SGD DESC:Epsilon subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:BFR1(YOR198C)|FD-Score:4.17|P-value:1.49E-5||SGD DESC:Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity Gene:BTS1(YPL069C)|FD-Score:4.01|P-value:2.99E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CAD1(YDR423C)|FD-Score:3.12|P-value:9.09E-4||SGD DESC:AP-1-like basic leucine zipper (bZIP) transcriptional activator; involved in stress responses, iron metabolism, and pleiotropic drug resistance; controls a set of genes involved in stabilizing proteins; binds consensus sequence TTACTAA; CAD1 has a paralog, YAP1, that arose from the whole genome duplication Gene:CDC55(YGL190C)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:Non-essential regulatory subunit B of protein phosphatase 2A (PP2A); localization to the cytoplasm requires Zds1p and Zds2p and promotes mitotic entry; localization to the nucleus prevents mitotic exit; required for correct nuclear division and chromosome segregation in meiosis; maintains nucleolar sequestration of Cdc14p during early meiosis; limits formation of PP2A-Rts1p holocomplexes to ensure timely dissolution of sister chromosome cohesion Gene:COG7(YGL005C)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DSE3(YOR264W)|FD-Score:-3.45|P-value:2.76E-4||SGD DESC:Daughter cell-specific protein, may help establish daughter fate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:FAP1(YNL023C)|FD-Score:3.91|P-value:4.61E-5||SGD DESC:Protein that binds to Fpr1p, conferring rapamycin resistance by competing with rapamycin for Fpr1p binding; accumulates in the nucleus upon treatment of cells with rapamycin; has similarity to D. melanogaster shuttle craft and human NFX1 Gene:FPR4(YLR449W)|FD-Score:6.46|P-value:5.16E-11||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase) (proline isomerase) localized to the nucleus; catalyzes isomerization of proline residues in histones H3 and H4, which affects lysine methylation of those histones Gene:FUB1(YCR076C)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:Putative protein of unknown function; interacts physically with multiple subunits of the 20S proteasome and genetically with genes encoding 20S core particle and 19S regulatory particle subunits; exhibits boundary activity which blocks the propagation of heterochromatic silencing; contains a PI31 proteasome regulator domain and sequence similarity with human PSMF1, a proteasome inhibitor; not an essential gene Gene:FYV10(YIL097W)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:Subunit of GID complex; involved in proteasome-dependent catabolite inactivation of gluconeogenic enzymes FBPase, PEPCK, and c-MDH; forms dimer with Rmd5p that is then recruited to GID Complex by Gid8p; contains a degenerate RING finger motif needed for GID complex ubiquitin ligase activity in vivo, as well as CTLH and CRA domains; plays role in anti-apoptosis; required for survival upon exposure to K1 killer toxin Gene:IES5(YER092W)|FD-Score:-3.95|P-value:3.85E-5||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions Gene:LCL3(YGL085W_p)|FD-Score:-3.22|P-value:6.41E-4||SGD DESC:Putative protein of unknown function; mutant has long chronological lifespan; has homology to Staphylococcus aureus nuclease; GFP-fusion protein localizes to mitochondria; is induced in response to the DNA-damaging agent MMS Gene:LST7(YGR057C)|FD-Score:5.18|P-value:1.11E-7||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:LYP1(YNL268W)|FD-Score:3.97|P-value:3.56E-5||SGD DESC:Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids Gene:MDM31(YHR194W)|FD-Score:4.37|P-value:6.19E-6||SGD DESC:Mitochondrial protein that may have a role in phospholipid metabolism; inner membrane protein with similarity to Mdm32p; required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MMM2, MDM10, MDM12, and MDM34 Gene:MFT1(YML062C)|FD-Score:3.11|P-value:9.38E-4||SGD DESC:Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance Gene:MNL1(YHR204W)|FD-Score:3.97|P-value:3.52E-5||SGD DESC:Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; in complex with Pdi1p, generates a Man7GlcNac2 oligosaccharide signal on glycoproteins destined for ubiquitin-proteasome degradation Gene:MNR2(YKL064W)|FD-Score:4.71|P-value:1.26E-6||SGD DESC:Vacuolar membrane protein required for magnesium homeostasis; putative magnesium transporter; has similarity to Alr1p and Alr2p, which mediate influx of Mg2+ and other divalent cations Gene:MOG1(YJR074W)|FD-Score:3.78|P-value:7.84E-5||SGD DESC:Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p Gene:MSK1(YNL073W)|FD-Score:-5.39|P-value:3.50E-8||SGD DESC:Mitochondrial lysine-tRNA synthetase, required for import of both aminoacylated and deacylated forms of tRNA(Lys) into mitochondria and for aminoacylation of mitochondrially encoded tRNA(Lys) Gene:MST1(YKL194C)|FD-Score:6.6|P-value:2.10E-11||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:NGL2(YMR285C)|FD-Score:4.43|P-value:4.62E-6||SGD DESC:Protein involved in 5.8S rRNA processing; Ccr4p-like RNase required for correct 3'-end formation of 5.8S rRNA at site E; similar to Ngl1p; NGL2 has a paralog, NGL3, that arose from the whole genome duplication Gene:NOP16(YER002W)|FD-Score:-3.09|P-value:9.93E-4||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis Gene:NUP84(YDL116W)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP107 Gene:OAZ1(YPL052W)|FD-Score:-3.9|P-value:4.79E-5||SGD DESC:Regulator of ornithine decarboxylase (Spe1p), antizyme that binds to Spe1p to stimulate ubiquitin-independent degradation by the proteasome; binding of polyamines to nascent Oaz1p during translation stimulates +1 ribosomal frameshifting, allowing translation of full-length Oaz1p Gene:PEX7(YDR142C)|FD-Score:3.86|P-value:5.77E-5||SGD DESC:Peroxisomal signal receptor for the N-terminal nonapeptide signal (PTS2) of peroxisomal matrix proteins; WD repeat protein; defects in human homolog cause lethal rhizomelic chondrodysplasia punctata (RCDP) Gene:PIB2(YGL023C)|FD-Score:3.09|P-value:9.94E-4||SGD DESC:Protein of unknown function; contains FYVE domain; similar to Fab1 and Vps27 Gene:PLP1(YDR183W)|FD-Score:4.47|P-value:3.84E-6||SGD DESC:Protein that interacts with CCT (chaperonin containing TCP-1) complex and has a role in actin and tubulin folding; has weak similarity to phosducins, which are G-protein regulators Gene:PSP2(YML017W)|FD-Score:-4.9|P-value:4.91E-7||SGD DESC:Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing Gene:PTP2(YOR208W)|FD-Score:4.38|P-value:6.01E-6||SGD DESC:Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the nucleus Gene:RMA1(YKL132C)|FD-Score:3.26|P-value:5.57E-4||SGD DESC:Putative dihydrofolate synthetase; has similarity to Fol3p and to E. coli folylpolyglutamate synthetase/dihydrofolate synthetase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RPL37B(YDR500C)|FD-Score:-3.33|P-value:4.40E-4||SGD DESC:Ribosomal 60S subunit protein L37B; protein abundance increases in response to DNA replication stress; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37B has a paralog, RPL37A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:6.35|P-value:1.09E-10||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS0A(YGR214W)|FD-Score:4.35|P-value:6.93E-6||SGD DESC:Ribosomal 40S subunit protein S0A; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2; RPS0A has a paralog, RPS0B, that arose from the whole genome duplication; Gene:RTC2(YBR147W)|FD-Score:-3.53|P-value:2.06E-4||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; positive regulation by Lys14p suggests that lysine may be the primary substrate; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter Gene:RUD3(YOR216C)|FD-Score:3.18|P-value:7.44E-4||SGD DESC:Golgi matrix protein involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1 Gene:SAC6(YDR129C)|FD-Score:-3.21|P-value:6.69E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SAM2(YDR502C)|FD-Score:3.25|P-value:5.74E-4||SGD DESC:S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM2 has a paralog, SAM1, that arose from the whole genome duplication Gene:SAS5(YOR213C)|FD-Score:6.08|P-value:6.10E-10||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity Gene:SDT1(YGL224C)|FD-Score:-3.21|P-value:6.71E-4||SGD DESC:Pyrimidine nucleotidase; overexpression suppresses the 6-AU sensitivity of transcription elongation factor S-II, as well as resistance to other pyrimidine derivatives Gene:SGF73(YGL066W)|FD-Score:5.55|P-value:1.39E-8||SGD DESC:SAGA complex subunit; has a role in anchoring the deubiquitination module into SAGA and SLIK complexes; involved in preinitiation complex assembly at promoters; human ortholog ataxin-7 is associated with spinocerebellar ataxia diseases; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay Gene:SNF1(YDR477W)|FD-Score:-4|P-value:3.20E-5||SGD DESC:AMP-activated serine/threonine protein kinase; found in a complex containing Snf4p and members of the Sip1p/Sip2p/Gal83p family; required for transcription of glucose-repressed genes, thermotolerance, sporulation, and peroxisome biogenesis; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; regulates filamentous growth in response to starvation Gene:SNQ2(YDR011W)|FD-Score:-3.69|P-value:1.13E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SUM1(YDR310C)|FD-Score:4.32|P-value:7.66E-6||SGD DESC:Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint Gene:SVF1(YDR346C)|FD-Score:3.09|P-value:9.98E-4||SGD DESC:Protein with a potential role in cell survival pathways, required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance Gene:TRP4(YDR354W)|FD-Score:7.72|P-value:5.63E-15||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSL1(YML100W)|FD-Score:3.11|P-value:9.25E-4||SGD DESC:Large subunit of trehalose 6-phosphate synthase/phosphatase complex; Tps1p-Tps2p complex converts uridine-5'-diphosphoglucose and glucose 6-phosphate to trehalose; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress; TSL1 has a paralog, TPS3, that arose from the whole genome duplication Gene:UNG1(YML021C)|FD-Score:4.21|P-value:1.26E-5||SGD DESC:Uracil-DNA glycosylase, required for repair of uracil in DNA formed by spontaneous cytosine deamination, not required for strand-specific mismatch repair, cell-cycle regulated, expressed in late G1, localizes to mitochondria and nucleus Gene:VPS29(YHR012W)|FD-Score:3.11|P-value:9.25E-4||SGD DESC:Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval Gene:WHI4(YDL224C)|FD-Score:-3.28|P-value:5.19E-4||SGD DESC:Putative RNA binding protein; regulates the cell size requirement for passage through Start and commitment to cell division; WHI4 has a paralog, WHI3, that arose from the whole genome duplication Gene:YBR184W(YBR184W_p)|FD-Score:3.34|P-value:4.14E-4||SGD DESC:Putative protein of unknown function; YBR184W is not an essential gene Gene:YCF1(YDR135C)|FD-Score:3.3|P-value:4.79E-4||SGD DESC:Vacuolar glutathione S-conjugate transporter; member of the ATP-binding cassette family; has a role in detoxifying metals such as cadmium, mercury, and arsenite; also transports unconjugated bilirubin, selenodigluthatione, and oxidized glutathione; similar to human cystic fibrosis protein CFTR Gene:YDL057W(YDL057W_p)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Putative protein of unknown function; YDL057W is not an essential gene Gene:YDL242W(YDL242W_d)|FD-Score:-3.14|P-value:8.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR250C(YDR250C_d)|FD-Score:-3.57|P-value:1.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YDR391C(YDR391C_p)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Putative protein of unknown function, possibly involved in zinc homeostasis; Bdf1p-dependent transcription induced by salt stress; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YEN1(YER041W)|FD-Score:4.4|P-value:5.35E-6||SGD DESC:Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p Gene:YER091C-A(YER091C-A_d)|FD-Score:3.83|P-value:6.38E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER135C(YER135C_d)|FD-Score:-4.59|P-value:2.18E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein; YER135C is not an essential gene Gene:YFL012W(YFL012W_p)|FD-Score:3.79|P-value:7.54E-5||SGD DESC:Putative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin Gene:YGR164W(YGR164W_d)|FD-Score:-4.09|P-value:2.15E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR124C(YJR124C_p)|FD-Score:-4.51|P-value:3.21E-6||SGD DESC:Putative protein of unknown function; expression induced under calcium shortage Gene:YKL107W(YKL107W_p)|FD-Score:-5.47|P-value:2.30E-8||SGD DESC:Putative short-chain dehydrogenase/reductase; proposed to be a palmitoylated membrane protein Gene:YML122C(YML122C_d)|FD-Score:-3.39|P-value:3.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL068C(YPL068C_p)|FD-Score:3.21|P-value:6.63E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YPR078C(YPR078C_p)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Putative protein of unknown function; possible role in DNA metabolism and/or in genome stability; expression is heat-inducible Gene:ZRC1(YMR243C)|FD-Score:-4.12|P-value:1.86E-5||SGD DESC:Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication Gene:ADR1(YDR216W)|FD-Score:3.14|P-value:8.34E-4||SGD DESC:Carbon source-responsive zinc-finger transcription factor, required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization Gene:AIM11(YER093C-A)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Protein of unknown function; null mutant is viable but shows increased loss of mitochondrial genome and synthetic interaction with prohibitin (phb1); contains an intron; YER093C-A has a paralog, YBL059W, that arose from the whole genome duplication Gene:ARO7(YPR060C)|FD-Score:3.23|P-value:6.15E-4||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:AST1(YBL069W)|FD-Score:3.72|P-value:9.95E-5||SGD DESC:Lipid raft associated protein; interacts with the plasma membrane ATPase Pma1p and has a role in its targeting to the plasma membrane by influencing its incorporation into lipid rafts; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST1 has a paralog, AST2, that arose from the whole genome duplication Gene:ATP15(YPL271W)|FD-Score:3.25|P-value:5.73E-4||SGD DESC:Epsilon subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:BFR1(YOR198C)|FD-Score:4.17|P-value:1.49E-5||SGD DESC:Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity Gene:BTS1(YPL069C)|FD-Score:4.01|P-value:2.99E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CAD1(YDR423C)|FD-Score:3.12|P-value:9.09E-4||SGD DESC:AP-1-like basic leucine zipper (bZIP) transcriptional activator; involved in stress responses, iron metabolism, and pleiotropic drug resistance; controls a set of genes involved in stabilizing proteins; binds consensus sequence TTACTAA; CAD1 has a paralog, YAP1, that arose from the whole genome duplication Gene:CDC55(YGL190C)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:Non-essential regulatory subunit B of protein phosphatase 2A (PP2A); localization to the cytoplasm requires Zds1p and Zds2p and promotes mitotic entry; localization to the nucleus prevents mitotic exit; required for correct nuclear division and chromosome segregation in meiosis; maintains nucleolar sequestration of Cdc14p during early meiosis; limits formation of PP2A-Rts1p holocomplexes to ensure timely dissolution of sister chromosome cohesion Gene:COG7(YGL005C)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DSE3(YOR264W)|FD-Score:-3.45|P-value:2.76E-4||SGD DESC:Daughter cell-specific protein, may help establish daughter fate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:FAP1(YNL023C)|FD-Score:3.91|P-value:4.61E-5||SGD DESC:Protein that binds to Fpr1p, conferring rapamycin resistance by competing with rapamycin for Fpr1p binding; accumulates in the nucleus upon treatment of cells with rapamycin; has similarity to D. melanogaster shuttle craft and human NFX1 Gene:FPR4(YLR449W)|FD-Score:6.46|P-value:5.16E-11||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase) (proline isomerase) localized to the nucleus; catalyzes isomerization of proline residues in histones H3 and H4, which affects lysine methylation of those histones Gene:FUB1(YCR076C)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:Putative protein of unknown function; interacts physically with multiple subunits of the 20S proteasome and genetically with genes encoding 20S core particle and 19S regulatory particle subunits; exhibits boundary activity which blocks the propagation of heterochromatic silencing; contains a PI31 proteasome regulator domain and sequence similarity with human PSMF1, a proteasome inhibitor; not an essential gene Gene:FYV10(YIL097W)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:Subunit of GID complex; involved in proteasome-dependent catabolite inactivation of gluconeogenic enzymes FBPase, PEPCK, and c-MDH; forms dimer with Rmd5p that is then recruited to GID Complex by Gid8p; contains a degenerate RING finger motif needed for GID complex ubiquitin ligase activity in vivo, as well as CTLH and CRA domains; plays role in anti-apoptosis; required for survival upon exposure to K1 killer toxin Gene:IES5(YER092W)|FD-Score:-3.95|P-value:3.85E-5||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions Gene:LCL3(YGL085W_p)|FD-Score:-3.22|P-value:6.41E-4||SGD DESC:Putative protein of unknown function; mutant has long chronological lifespan; has homology to Staphylococcus aureus nuclease; GFP-fusion protein localizes to mitochondria; is induced in response to the DNA-damaging agent MMS Gene:LST7(YGR057C)|FD-Score:5.18|P-value:1.11E-7||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:LYP1(YNL268W)|FD-Score:3.97|P-value:3.56E-5||SGD DESC:Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids Gene:MDM31(YHR194W)|FD-Score:4.37|P-value:6.19E-6||SGD DESC:Mitochondrial protein that may have a role in phospholipid metabolism; inner membrane protein with similarity to Mdm32p; required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MMM2, MDM10, MDM12, and MDM34 Gene:MFT1(YML062C)|FD-Score:3.11|P-value:9.38E-4||SGD DESC:Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance Gene:MNL1(YHR204W)|FD-Score:3.97|P-value:3.52E-5||SGD DESC:Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; in complex with Pdi1p, generates a Man7GlcNac2 oligosaccharide signal on glycoproteins destined for ubiquitin-proteasome degradation Gene:MNR2(YKL064W)|FD-Score:4.71|P-value:1.26E-6||SGD DESC:Vacuolar membrane protein required for magnesium homeostasis; putative magnesium transporter; has similarity to Alr1p and Alr2p, which mediate influx of Mg2+ and other divalent cations Gene:MOG1(YJR074W)|FD-Score:3.78|P-value:7.84E-5||SGD DESC:Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p Gene:MSK1(YNL073W)|FD-Score:-5.39|P-value:3.50E-8||SGD DESC:Mitochondrial lysine-tRNA synthetase, required for import of both aminoacylated and deacylated forms of tRNA(Lys) into mitochondria and for aminoacylation of mitochondrially encoded tRNA(Lys) Gene:MST1(YKL194C)|FD-Score:6.6|P-value:2.10E-11||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:NGL2(YMR285C)|FD-Score:4.43|P-value:4.62E-6||SGD DESC:Protein involved in 5.8S rRNA processing; Ccr4p-like RNase required for correct 3'-end formation of 5.8S rRNA at site E; similar to Ngl1p; NGL2 has a paralog, NGL3, that arose from the whole genome duplication Gene:NOP16(YER002W)|FD-Score:-3.09|P-value:9.93E-4||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis Gene:NUP84(YDL116W)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP107 Gene:OAZ1(YPL052W)|FD-Score:-3.9|P-value:4.79E-5||SGD DESC:Regulator of ornithine decarboxylase (Spe1p), antizyme that binds to Spe1p to stimulate ubiquitin-independent degradation by the proteasome; binding of polyamines to nascent Oaz1p during translation stimulates +1 ribosomal frameshifting, allowing translation of full-length Oaz1p Gene:PEX7(YDR142C)|FD-Score:3.86|P-value:5.77E-5||SGD DESC:Peroxisomal signal receptor for the N-terminal nonapeptide signal (PTS2) of peroxisomal matrix proteins; WD repeat protein; defects in human homolog cause lethal rhizomelic chondrodysplasia punctata (RCDP) Gene:PIB2(YGL023C)|FD-Score:3.09|P-value:9.94E-4||SGD DESC:Protein of unknown function; contains FYVE domain; similar to Fab1 and Vps27 Gene:PLP1(YDR183W)|FD-Score:4.47|P-value:3.84E-6||SGD DESC:Protein that interacts with CCT (chaperonin containing TCP-1) complex and has a role in actin and tubulin folding; has weak similarity to phosducins, which are G-protein regulators Gene:PSP2(YML017W)|FD-Score:-4.9|P-value:4.91E-7||SGD DESC:Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing Gene:PTP2(YOR208W)|FD-Score:4.38|P-value:6.01E-6||SGD DESC:Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the nucleus Gene:RMA1(YKL132C)|FD-Score:3.26|P-value:5.57E-4||SGD DESC:Putative dihydrofolate synthetase; has similarity to Fol3p and to E. coli folylpolyglutamate synthetase/dihydrofolate synthetase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RPL37B(YDR500C)|FD-Score:-3.33|P-value:4.40E-4||SGD DESC:Ribosomal 60S subunit protein L37B; protein abundance increases in response to DNA replication stress; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37B has a paralog, RPL37A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:6.35|P-value:1.09E-10||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS0A(YGR214W)|FD-Score:4.35|P-value:6.93E-6||SGD DESC:Ribosomal 40S subunit protein S0A; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2; RPS0A has a paralog, RPS0B, that arose from the whole genome duplication; Gene:RTC2(YBR147W)|FD-Score:-3.53|P-value:2.06E-4||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; positive regulation by Lys14p suggests that lysine may be the primary substrate; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter Gene:RUD3(YOR216C)|FD-Score:3.18|P-value:7.44E-4||SGD DESC:Golgi matrix protein involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1 Gene:SAC6(YDR129C)|FD-Score:-3.21|P-value:6.69E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SAM2(YDR502C)|FD-Score:3.25|P-value:5.74E-4||SGD DESC:S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM2 has a paralog, SAM1, that arose from the whole genome duplication Gene:SAS5(YOR213C)|FD-Score:6.08|P-value:6.10E-10||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity Gene:SDT1(YGL224C)|FD-Score:-3.21|P-value:6.71E-4||SGD DESC:Pyrimidine nucleotidase; overexpression suppresses the 6-AU sensitivity of transcription elongation factor S-II, as well as resistance to other pyrimidine derivatives Gene:SGF73(YGL066W)|FD-Score:5.55|P-value:1.39E-8||SGD DESC:SAGA complex subunit; has a role in anchoring the deubiquitination module into SAGA and SLIK complexes; involved in preinitiation complex assembly at promoters; human ortholog ataxin-7 is associated with spinocerebellar ataxia diseases; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay Gene:SNF1(YDR477W)|FD-Score:-4|P-value:3.20E-5||SGD DESC:AMP-activated serine/threonine protein kinase; found in a complex containing Snf4p and members of the Sip1p/Sip2p/Gal83p family; required for transcription of glucose-repressed genes, thermotolerance, sporulation, and peroxisome biogenesis; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; regulates filamentous growth in response to starvation Gene:SNQ2(YDR011W)|FD-Score:-3.69|P-value:1.13E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SUM1(YDR310C)|FD-Score:4.32|P-value:7.66E-6||SGD DESC:Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint Gene:SVF1(YDR346C)|FD-Score:3.09|P-value:9.98E-4||SGD DESC:Protein with a potential role in cell survival pathways, required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance Gene:TRP4(YDR354W)|FD-Score:7.72|P-value:5.63E-15||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSL1(YML100W)|FD-Score:3.11|P-value:9.25E-4||SGD DESC:Large subunit of trehalose 6-phosphate synthase/phosphatase complex; Tps1p-Tps2p complex converts uridine-5'-diphosphoglucose and glucose 6-phosphate to trehalose; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress; TSL1 has a paralog, TPS3, that arose from the whole genome duplication Gene:UNG1(YML021C)|FD-Score:4.21|P-value:1.26E-5||SGD DESC:Uracil-DNA glycosylase, required for repair of uracil in DNA formed by spontaneous cytosine deamination, not required for strand-specific mismatch repair, cell-cycle regulated, expressed in late G1, localizes to mitochondria and nucleus Gene:VPS29(YHR012W)|FD-Score:3.11|P-value:9.25E-4||SGD DESC:Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval Gene:WHI4(YDL224C)|FD-Score:-3.28|P-value:5.19E-4||SGD DESC:Putative RNA binding protein; regulates the cell size requirement for passage through Start and commitment to cell division; WHI4 has a paralog, WHI3, that arose from the whole genome duplication Gene:YBR184W(YBR184W_p)|FD-Score:3.34|P-value:4.14E-4||SGD DESC:Putative protein of unknown function; YBR184W is not an essential gene Gene:YCF1(YDR135C)|FD-Score:3.3|P-value:4.79E-4||SGD DESC:Vacuolar glutathione S-conjugate transporter; member of the ATP-binding cassette family; has a role in detoxifying metals such as cadmium, mercury, and arsenite; also transports unconjugated bilirubin, selenodigluthatione, and oxidized glutathione; similar to human cystic fibrosis protein CFTR Gene:YDL057W(YDL057W_p)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Putative protein of unknown function; YDL057W is not an essential gene Gene:YDL242W(YDL242W_d)|FD-Score:-3.14|P-value:8.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR250C(YDR250C_d)|FD-Score:-3.57|P-value:1.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YDR391C(YDR391C_p)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Putative protein of unknown function, possibly involved in zinc homeostasis; Bdf1p-dependent transcription induced by salt stress; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YEN1(YER041W)|FD-Score:4.4|P-value:5.35E-6||SGD DESC:Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p Gene:YER091C-A(YER091C-A_d)|FD-Score:3.83|P-value:6.38E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER135C(YER135C_d)|FD-Score:-4.59|P-value:2.18E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein; YER135C is not an essential gene Gene:YFL012W(YFL012W_p)|FD-Score:3.79|P-value:7.54E-5||SGD DESC:Putative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin Gene:YGR164W(YGR164W_d)|FD-Score:-4.09|P-value:2.15E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR124C(YJR124C_p)|FD-Score:-4.51|P-value:3.21E-6||SGD DESC:Putative protein of unknown function; expression induced under calcium shortage Gene:YKL107W(YKL107W_p)|FD-Score:-5.47|P-value:2.30E-8||SGD DESC:Putative short-chain dehydrogenase/reductase; proposed to be a palmitoylated membrane protein Gene:YML122C(YML122C_d)|FD-Score:-3.39|P-value:3.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL068C(YPL068C_p)|FD-Score:3.21|P-value:6.63E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YPR078C(YPR078C_p)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Putative protein of unknown function; possible role in DNA metabolism and/or in genome stability; expression is heat-inducible Gene:ZRC1(YMR243C)|FD-Score:-4.12|P-value:1.86E-5||SGD DESC:Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR190W4.914.67E-70.50ERG9Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway
YGL099W4.681.46E-60.50LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YDR353W4.171.50E-50.16TRR1Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication
YDL217C4.013.02E-50.15TIM22Essential core component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported
YHR107C3.865.57E-50.10CDC12Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YDR498C3.768.41E-50.09SEC20Membrane glycoprotein v-SNARE involved in retrograde transport from the Golgi to the ER; required for N- and O-glycosylation in the Golgi but not in the ER; interacts with the Dsl1p complex through Tip20p
YLR009W3.681.19E-40.18RLP24Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp
YPL235W3.492.39E-40.06RVB2ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly
YJR002W3.433.03E-40.01MPP10Component of the SSU processome and 90S preribosome, required for pre-18S rRNA processing, interacts with and controls the stability of Imp3p and Imp4p, essential for viability; similar to human Mpp10p
YBL018C3.423.10E-40.04POP8Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YPL204W3.383.58E-40.06HRR25Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta)
YER112W3.334.42E-40.17LSM4Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress
YER043C3.158.13E-40.04SAH1S-adenosyl-L-homocysteine hydrolase, catabolizes S-adenosyl-L-homocysteine which is formed after donation of the activated methyl group of S-adenosyl-L-methionine (AdoMet) to an acceptor
YPR186C3.119.44E-40.04PZF1Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA
YML105C3.070.001070.02SEC65Subunit of the signal recognition particle (SRP), involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR354W7.725.63E-15TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YKL194C6.602.10E-11MST1Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro
YLR449W6.465.16E-11FPR4Peptidyl-prolyl cis-trans isomerase (PPIase) (proline isomerase) localized to the nucleus; catalyzes isomerization of proline residues in histones H3 and H4, which affects lysine methylation of those histones
YDL020C6.351.09E-10RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YOR213C6.086.10E-10SAS5Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity
YGL066W5.551.39E-8SGF73SAGA complex subunit; has a role in anchoring the deubiquitination module into SAGA and SLIK complexes; involved in preinitiation complex assembly at promoters; human ortholog ataxin-7 is associated with spinocerebellar ataxia diseases; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay
YGR057C5.181.11E-7LST7Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface
YKL064W4.711.26E-6MNR2Vacuolar membrane protein required for magnesium homeostasis; putative magnesium transporter; has similarity to Alr1p and Alr2p, which mediate influx of Mg2+ and other divalent cations
YDR183W4.473.84E-6PLP1Protein that interacts with CCT (chaperonin containing TCP-1) complex and has a role in actin and tubulin folding; has weak similarity to phosducins, which are G-protein regulators
YMR285C4.434.62E-6NGL2Protein involved in 5.8S rRNA processing; Ccr4p-like RNase required for correct 3'-end formation of 5.8S rRNA at site E; similar to Ngl1p; NGL2 has a paralog, NGL3, that arose from the whole genome duplication
YER041W4.405.35E-6YEN1Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p
YOR208W4.386.01E-6PTP2Phosphotyrosine-specific protein phosphatase involved in the inactivation of mitogen-activated protein kinase (MAPK) during osmolarity sensing; dephosporylates Hog1p MAPK and regulates its localization; localized to the nucleus
YHR194W4.376.19E-6MDM31Mitochondrial protein that may have a role in phospholipid metabolism; inner membrane protein with similarity to Mdm32p; required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MMM2, MDM10, MDM12, and MDM34
YGR214W4.356.93E-6RPS0ARibosomal 40S subunit protein S0A; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2; RPS0A has a paralog, RPS0B, that arose from the whole genome duplication;
YDR310C4.327.66E-6SUM1Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint

GO enrichment analysis for SGTC_2811
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1019.83E-15SGTC_2735sertaconazole 140.0 nMMiscellaneous658630.0851064plasma membrane duress
0.0921.28E-12SGTC_23859074692 200.0 μMChembridge (Fragment library)170469060.0769231
0.0895.79E-12SGTC_22997976368 114.9 μMChembridge (Fragment library)29797180.0533333
0.0881.64E-11SGTC_21945927483 104.5 μMChembridge (Fragment library)7609790.132353
0.0872.93E-11SGTC_22067254556 147.0 μMChembridge (Fragment library)43989050.16666760S ribosome export
0.0831.64E-10SGTC_26602',4'-dihydroxychalcone 17.1 μMTimTec (Pure natural product library)53572180.1
0.0831.82E-10SGTC_29187977171 22.1 μMChembridge (Drug-like library)22364830.155844
0.0813.93E-10SGTC_676falcarinol 140.0 μMMiscellaneous33220.0123457
0.0814.86E-10SGTC_23127706453 155.7 μMChembridge (Fragment library)9760980.097561
0.0815.00E-10SGTC_305-(2-thienylidene)-Rhodanine 28.7 μMChemDiv (Drug-like library)12411320.142857
0.0806.92E-10SGTC_14740330-0125 5.3 μMChemDiv (Drug-like library)X14740.0853659
0.0808.68E-10SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.0487805
0.0799.95E-10SGTC_2733amorolfine 100.0 μMMiscellaneous542600.0481928plasma membrane duress
0.0791.19E-9SGTC_1070indatraline 8.8 μMNIH Clinical Collection103144720.094594660S ribosome export
0.0782.06E-9SGTC_240417-1663 219.0 μMChemDiv (Drug-like library)43293900.11236

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_20535265679138 μM0.557692764595Chembridge (Fragment library)233.286242.55113
SGTC_3204911418349.47 μM0.52727317027248Chembridge (Drug-like library)329.2463533.40317
SGTC_3187910939449.47 μM0.43333317098090Chembridge (Drug-like library)247.312822.913Golgi
SGTC_3189911068249.47 μM0.4062517122418Chembridge (Drug-like library)273.35013.61413
SGTC_3007907838371.43 μM0.397266496995Chembridge (Drug-like library)351.379123.04716
SGTC_11413253-038952.6 μM0.3787885402031ChemDiv (Drug-like library)290.337562.60315
SGTC_2810795987145.46 μM0.3714292974535Chembridge (Drug-like library)333.40694.35213copper-dependent oxidative stress
SGTC_6293448-646676.7 μM0.370968573744ChemDiv (Drug-like library)254.3072.75413
SGTC_9143820-6779408 μM0.3484852893481ChemDiv (Drug-like library)392.450884.69725
SGTC_2061524588480.95 μM0.344828573863Chembridge (Fragment library)210.276041.56514tubulin folding & SWR complex