7989630

N-(5-chloro-2-phenoxyphenyl)-4-(methanesulfonamido)benzamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2820
Screen concentration 71.4 μM
Source Chembridge (Drug-like library)
PubChem CID 2984098
SMILES CS(=O)(=O)NC1=CC=C(C=C1)C(=O)NC2=C(C=CC(=C2)Cl)OC3=CC=CC=C3
Standardized SMILES CS(=O)(=O)Nc1ccc(cc1)C(=O)Nc2cc(Cl)ccc2Oc3ccccc3
Molecular weight 416.878
ALogP 3.75
H-bond donor count 2
H-bond acceptor count 5
Response signature ERG2

Pool Growth Kinetics
% growth inhibition (Het. pool) 4.81
% growth inhibition (Hom. pool) -0.02


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2984098
Download HIP data (tab-delimited text)  (excel)
Gene:ARB1(YER036C)|FD-Score:3.35|P-value:3.97E-4|Clearance:0.09||SGD DESC:ATPase of the ATP-binding cassette (ABC) family involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p Gene:ARC40(YBR234C)|FD-Score:3.83|P-value:6.35E-5|Clearance:0.23||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:BRX1(YOL077C)|FD-Score:-3.21|P-value:6.64E-4|Clearance:0||SGD DESC:Nucleolar protein, constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif Gene:GLN1(YPR035W)|FD-Score:-3.37|P-value:3.82E-4|Clearance:0||SGD DESC:Glutamine synthetase (GS); synthesizes glutamine from glutamate and ammonia; with Glt1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source and by amino acid limitation; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:LSG1(YGL099W)|FD-Score:3.98|P-value:3.45E-5|Clearance:0.12||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:PRE4(YFR050C)|FD-Score:5.47|P-value:2.24E-8|Clearance:1.2||SGD DESC:Beta 7 subunit of the 20S proteasome Gene:PRP31(YGR091W)|FD-Score:3.14|P-value:8.56E-4|Clearance:0.13||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RPN7(YPR108W)|FD-Score:-3.14|P-value:8.38E-4|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits Gene:RRP15(YPR143W)|FD-Score:3.6|P-value:1.58E-4|Clearance:0.25||SGD DESC:Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs Gene:RRP45(YDR280W)|FD-Score:3.27|P-value:5.42E-4|Clearance:0.13||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:SES1(YDR023W)|FD-Score:4.27|P-value:9.65E-6|Clearance:0.29||SGD DESC:Cytosolic seryl-tRNA synthetase, class II aminoacyl-tRNA synthetase that aminoacylates tRNA(Ser), displays tRNA-dependent amino acid recognition which enhances discrimination of the serine substrate, interacts with peroxin Pex21p Gene:SSL1(YLR005W)|FD-Score:-3.35|P-value:4.02E-4|Clearance:0||SGD DESC:Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p Gene:SWI1(YPL016W)|FD-Score:-3.37|P-value:3.71E-4|Clearance:0||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer Gene:YDR526C(YDR526C_d)|FD-Score:3.86|P-value:5.68E-5|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL009W(YJL009W_d)|FD-Score:-3.29|P-value:4.95E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex Gene:YPT1(YFL038C)|FD-Score:-5.59|P-value:1.13E-8|Clearance:0||SGD DESC:Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p) Gene:ARB1(YER036C)|FD-Score:3.35|P-value:3.97E-4|Clearance:0.09||SGD DESC:ATPase of the ATP-binding cassette (ABC) family involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p Gene:ARC40(YBR234C)|FD-Score:3.83|P-value:6.35E-5|Clearance:0.23||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:BRX1(YOL077C)|FD-Score:-3.21|P-value:6.64E-4|Clearance:0||SGD DESC:Nucleolar protein, constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif Gene:GLN1(YPR035W)|FD-Score:-3.37|P-value:3.82E-4|Clearance:0||SGD DESC:Glutamine synthetase (GS); synthesizes glutamine from glutamate and ammonia; with Glt1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source and by amino acid limitation; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:LSG1(YGL099W)|FD-Score:3.98|P-value:3.45E-5|Clearance:0.12||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:PRE4(YFR050C)|FD-Score:5.47|P-value:2.24E-8|Clearance:1.2||SGD DESC:Beta 7 subunit of the 20S proteasome Gene:PRP31(YGR091W)|FD-Score:3.14|P-value:8.56E-4|Clearance:0.13||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:RPN7(YPR108W)|FD-Score:-3.14|P-value:8.38E-4|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits Gene:RRP15(YPR143W)|FD-Score:3.6|P-value:1.58E-4|Clearance:0.25||SGD DESC:Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs Gene:RRP45(YDR280W)|FD-Score:3.27|P-value:5.42E-4|Clearance:0.13||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:SES1(YDR023W)|FD-Score:4.27|P-value:9.65E-6|Clearance:0.29||SGD DESC:Cytosolic seryl-tRNA synthetase, class II aminoacyl-tRNA synthetase that aminoacylates tRNA(Ser), displays tRNA-dependent amino acid recognition which enhances discrimination of the serine substrate, interacts with peroxin Pex21p Gene:SSL1(YLR005W)|FD-Score:-3.35|P-value:4.02E-4|Clearance:0||SGD DESC:Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p Gene:SWI1(YPL016W)|FD-Score:-3.37|P-value:3.71E-4|Clearance:0||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer Gene:YDR526C(YDR526C_d)|FD-Score:3.86|P-value:5.68E-5|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL009W(YJL009W_d)|FD-Score:-3.29|P-value:4.95E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex Gene:YPT1(YFL038C)|FD-Score:-5.59|P-value:1.13E-8|Clearance:0||SGD DESC:Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p)

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2984098
Download HOP data (tab-delimited text)  (excel)
Gene:AIM26(YKL037W)|FD-Score:3.8|P-value:7.38E-5||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT Gene:ANS1(YHR126C_p)|FD-Score:-3.34|P-value:4.17E-4||SGD DESC:Putative GPI protein; transcription dependent upon Azf1p Gene:ASF1(YJL115W)|FD-Score:-3.12|P-value:9.13E-4||SGD DESC:Nucleosome assembly factor, involved in chromatin assembly and disassembly, anti-silencing protein that causes derepression of silent loci when overexpressed; plays a role in regulating Ty1 transposition Gene:AVT3(YKL146W)|FD-Score:-3.59|P-value:1.65E-4||SGD DESC:Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BFR1(YOR198C)|FD-Score:4.07|P-value:2.36E-5||SGD DESC:Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity Gene:BST1(YFL025C)|FD-Score:3.86|P-value:5.78E-5||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:CCM1(YGR150C)|FD-Score:-3.15|P-value:8.10E-4||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CHS5(YLR330W)|FD-Score:-3.35|P-value:3.99E-4||SGD DESC:Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane Gene:CNB1(YKL190W)|FD-Score:4.14|P-value:1.71E-5||SGD DESC:Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress Gene:CTF18(YMR078C)|FD-Score:3.77|P-value:8.20E-5||SGD DESC:Subunit of a complex with Ctf8p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion; may have overlapping functions with Rad24p in the DNA damage replication checkpoint Gene:CTI6(YPL181W)|FD-Score:3.26|P-value:5.57E-4||SGD DESC:Protein that relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter; contains a PHD finger domain Gene:CTM1(YHR109W)|FD-Score:-3.93|P-value:4.33E-5||SGD DESC:Cytochrome c lysine methyltransferase, trimethylates residue 72 of apo-cytochrome c (Cyc1p) in the cytosol; not required for normal respiratory growth Gene:DHH1(YDL160C)|FD-Score:3.93|P-value:4.23E-5||SGD DESC:Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, may have a role in mRNA export and translation; C-terminus of Dhh1p interacts with Ngr1p and promotes POR1, but not EDC1 mRNA decay; forms cytoplasmic foci upon DNA replication stress Gene:EEB1(YPL095C)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short-chain esterase activity; may be involved in lipid metabolism and detoxification; EEB1 has a paralog, EHT1, that arose from the whole genome duplication Gene:ELC1(YPL046C)|FD-Score:4.56|P-value:2.52E-6||SGD DESC:Elongin C, conserved among eukaryotes; forms a complex with Cul3p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; plays a role in global genomic repair Gene:ERG2(YMR202W)|FD-Score:18.2|P-value:1.14E-74||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG4(YGL012W)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FMO1(YHR176W)|FD-Score:-3.74|P-value:9.28E-5||SGD DESC:Flavin-containing monooxygenase, localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins Gene:GAL11(YOL051W)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GRR1(YJR090C)|FD-Score:3.26|P-value:5.61E-4||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:HER2(YMR293C)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; required for remodeling of ER caused by Hmg2p overexpression; similar to bacterial GatA glutamyl-tRNA amidotransferase Gene:IRS4(YKR019C)|FD-Score:3.83|P-value:6.39E-5||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LAP3(YNL239W)|FD-Score:3.86|P-value:5.60E-5||SGD DESC:Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH Gene:MNE1(YOR350C)|FD-Score:-3.47|P-value:2.64E-4||SGD DESC:Mitochondrial matrix protein involved in splicing Group I aI5-beta intron from COX1 mRNA Gene:NCS6(YGL211W)|FD-Score:-3.92|P-value:4.48E-5||SGD DESC:Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:PEX12(YMR026C)|FD-Score:-6.74|P-value:7.99E-12||SGD DESC:C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder Gene:PGD1(YGL025C)|FD-Score:5.63|P-value:8.84E-9||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PHO4(YFR034C)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability Gene:PHO89(YBR296C)|FD-Score:4.03|P-value:2.77E-5||SGD DESC:Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification Gene:POR2(YIL114C)|FD-Score:3.28|P-value:5.12E-4||SGD DESC:Putative mitochondrial porin (voltage-dependent anion channel); not required for mitochondrial membrane permeability or mitochondrial osmotic stability; POR2 has a paralog, POR1, that arose from the whole genome duplication Gene:PTR2(YKR093W)|FD-Score:5.09|P-value:1.82E-7||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:RNH202(YDR279W)|FD-Score:-3.19|P-value:7.08E-4||SGD DESC:Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS2 that causes Aicardi-Goutieres syndrome Gene:RPL14A(YKL006W)|FD-Score:3.85|P-value:6.00E-5||SGD DESC:Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication Gene:RPL1A(YPL220W)|FD-Score:4.29|P-value:9.10E-6||SGD DESC:Ribosomal 60S subunit protein L1A; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1A has a paralog, RPL1B, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal Gene:RPS18B(YML026C)|FD-Score:-3.1|P-value:9.82E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18B has a paralog, RPS18A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SHS1(YDL225W)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins; undergoes sumoylation and phosphorylation during mitosis; protein abundance increases in response to DNA replication stress Gene:SNF12(YNR023W)|FD-Score:4.23|P-value:1.15E-5||SGD DESC:73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive Gene:SSD1(YDR293C)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SSH1(YBR283C)|FD-Score:-4.29|P-value:8.81E-6||SGD DESC:Subunit of the Ssh1 translocon complex; Sec61p homolog involved in co-translational pathway of protein translocation; not essential Gene:STE24(YJR117W)|FD-Score:4.18|P-value:1.47E-5||SGD DESC:Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans Gene:SUR4(YLR372W)|FD-Score:5.25|P-value:7.48E-8||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SUS1(YBR111W-A)|FD-Score:3.19|P-value:7.04E-4||SGD DESC:Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP Gene:TRP2(YER090W)|FD-Score:3.84|P-value:6.26E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:VAM6(YDL077C)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p Gene:VMA1(YDL185W)|FD-Score:3.99|P-value:3.31E-5||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA16(YHR026W)|FD-Score:3.73|P-value:9.59E-5||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:YAP5(YIR018W)|FD-Score:-4.54|P-value:2.83E-6||SGD DESC:Basic leucine zipper (bZIP) iron-sensing transcription factor; YAP5 has a paralog, YAP7, that arose from the whole genome duplication Gene:YBL008W-A(YBL008W-A_p)|FD-Score:7.94|P-value:9.73E-16||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBR056W-A(YBR056W-A_p)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the dubious ORF YBR056C-B Gene:YCR064C(YCR064C_d)|FD-Score:5.52|P-value:1.72E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YEL028W(YEL028W_d)|FD-Score:4.4|P-value:5.31E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YFR012W-A(YFR012W-A_p)|FD-Score:4.78|P-value:8.88E-7||SGD DESC:Putative protein of unknown function; identified by homology Gene:YGR035W-A(YGR035W-A_p)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Putative protein of unknown function Gene:YGR064W(YGR064W_d)|FD-Score:3.17|P-value:7.54E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YGR168C(YGR168C_p)|FD-Score:3.16|P-value:8.00E-4||SGD DESC:Putative protein of unknown function; YGR168C is not an essential gene Gene:YIL055C(YIL055C_p)|FD-Score:3.17|P-value:7.75E-4||SGD DESC:Putative protein of unknown function Gene:YJL152W(YJL152W_d)|FD-Score:4.07|P-value:2.31E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR001C(YLR001C_p)|FD-Score:4.1|P-value:2.06E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; predicted to be palmitoylated Gene:YML057C-A(YML057C-A_d)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene CMP2/YML057W Gene:YML122C(YML122C_d)|FD-Score:5.07|P-value:1.99E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR245W(YMR245W_d)|FD-Score:3.88|P-value:5.28E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPK1(YKL126W)|FD-Score:5.62|P-value:9.57E-9||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPR170C(YPR170C_d)|FD-Score:-3.11|P-value:9.21E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B Gene:YPT7(YML001W)|FD-Score:3.95|P-value:3.89E-5||SGD DESC:Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7 Gene:AIM26(YKL037W)|FD-Score:3.8|P-value:7.38E-5||SGD DESC:Putative protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT Gene:ANS1(YHR126C_p)|FD-Score:-3.34|P-value:4.17E-4||SGD DESC:Putative GPI protein; transcription dependent upon Azf1p Gene:ASF1(YJL115W)|FD-Score:-3.12|P-value:9.13E-4||SGD DESC:Nucleosome assembly factor, involved in chromatin assembly and disassembly, anti-silencing protein that causes derepression of silent loci when overexpressed; plays a role in regulating Ty1 transposition Gene:AVT3(YKL146W)|FD-Score:-3.59|P-value:1.65E-4||SGD DESC:Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BFR1(YOR198C)|FD-Score:4.07|P-value:2.36E-5||SGD DESC:Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity Gene:BST1(YFL025C)|FD-Score:3.86|P-value:5.78E-5||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:CCM1(YGR150C)|FD-Score:-3.15|P-value:8.10E-4||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CHS5(YLR330W)|FD-Score:-3.35|P-value:3.99E-4||SGD DESC:Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane Gene:CNB1(YKL190W)|FD-Score:4.14|P-value:1.71E-5||SGD DESC:Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress Gene:CTF18(YMR078C)|FD-Score:3.77|P-value:8.20E-5||SGD DESC:Subunit of a complex with Ctf8p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion; may have overlapping functions with Rad24p in the DNA damage replication checkpoint Gene:CTI6(YPL181W)|FD-Score:3.26|P-value:5.57E-4||SGD DESC:Protein that relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter; contains a PHD finger domain Gene:CTM1(YHR109W)|FD-Score:-3.93|P-value:4.33E-5||SGD DESC:Cytochrome c lysine methyltransferase, trimethylates residue 72 of apo-cytochrome c (Cyc1p) in the cytosol; not required for normal respiratory growth Gene:DHH1(YDL160C)|FD-Score:3.93|P-value:4.23E-5||SGD DESC:Cytoplasmic DExD/H-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacts with both the decapping and deadenylase complexes, may have a role in mRNA export and translation; C-terminus of Dhh1p interacts with Ngr1p and promotes POR1, but not EDC1 mRNA decay; forms cytoplasmic foci upon DNA replication stress Gene:EEB1(YPL095C)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short-chain esterase activity; may be involved in lipid metabolism and detoxification; EEB1 has a paralog, EHT1, that arose from the whole genome duplication Gene:ELC1(YPL046C)|FD-Score:4.56|P-value:2.52E-6||SGD DESC:Elongin C, conserved among eukaryotes; forms a complex with Cul3p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; plays a role in global genomic repair Gene:ERG2(YMR202W)|FD-Score:18.2|P-value:1.14E-74||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG4(YGL012W)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FMO1(YHR176W)|FD-Score:-3.74|P-value:9.28E-5||SGD DESC:Flavin-containing monooxygenase, localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins Gene:GAL11(YOL051W)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GRR1(YJR090C)|FD-Score:3.26|P-value:5.61E-4||SGD DESC:F-box protein component of the SCF ubiquitin-ligase complex; involved in carbon catabolite repression, glucose-dependent divalent cation transport, high-affinity glucose transport, morphogenesis, and sulfite detoxification Gene:HER2(YMR293C)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; required for remodeling of ER caused by Hmg2p overexpression; similar to bacterial GatA glutamyl-tRNA amidotransferase Gene:IRS4(YKR019C)|FD-Score:3.83|P-value:6.39E-5||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LAP3(YNL239W)|FD-Score:3.86|P-value:5.60E-5||SGD DESC:Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH Gene:MNE1(YOR350C)|FD-Score:-3.47|P-value:2.64E-4||SGD DESC:Mitochondrial matrix protein involved in splicing Group I aI5-beta intron from COX1 mRNA Gene:NCS6(YGL211W)|FD-Score:-3.92|P-value:4.48E-5||SGD DESC:Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:PEX12(YMR026C)|FD-Score:-6.74|P-value:7.99E-12||SGD DESC:C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder Gene:PGD1(YGL025C)|FD-Score:5.63|P-value:8.84E-9||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PHO4(YFR034C)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability Gene:PHO89(YBR296C)|FD-Score:4.03|P-value:2.77E-5||SGD DESC:Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification Gene:POR2(YIL114C)|FD-Score:3.28|P-value:5.12E-4||SGD DESC:Putative mitochondrial porin (voltage-dependent anion channel); not required for mitochondrial membrane permeability or mitochondrial osmotic stability; POR2 has a paralog, POR1, that arose from the whole genome duplication Gene:PTR2(YKR093W)|FD-Score:5.09|P-value:1.82E-7||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:RNH202(YDR279W)|FD-Score:-3.19|P-value:7.08E-4||SGD DESC:Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS2 that causes Aicardi-Goutieres syndrome Gene:RPL14A(YKL006W)|FD-Score:3.85|P-value:6.00E-5||SGD DESC:Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication Gene:RPL1A(YPL220W)|FD-Score:4.29|P-value:9.10E-6||SGD DESC:Ribosomal 60S subunit protein L1A; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1A has a paralog, RPL1B, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal Gene:RPS18B(YML026C)|FD-Score:-3.1|P-value:9.82E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18B has a paralog, RPS18A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SHS1(YDL225W)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins; undergoes sumoylation and phosphorylation during mitosis; protein abundance increases in response to DNA replication stress Gene:SNF12(YNR023W)|FD-Score:4.23|P-value:1.15E-5||SGD DESC:73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive Gene:SSD1(YDR293C)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SSH1(YBR283C)|FD-Score:-4.29|P-value:8.81E-6||SGD DESC:Subunit of the Ssh1 translocon complex; Sec61p homolog involved in co-translational pathway of protein translocation; not essential Gene:STE24(YJR117W)|FD-Score:4.18|P-value:1.47E-5||SGD DESC:Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans Gene:SUR4(YLR372W)|FD-Score:5.25|P-value:7.48E-8||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SUS1(YBR111W-A)|FD-Score:3.19|P-value:7.04E-4||SGD DESC:Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP Gene:TRP2(YER090W)|FD-Score:3.84|P-value:6.26E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:VAM6(YDL077C)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p Gene:VMA1(YDL185W)|FD-Score:3.99|P-value:3.31E-5||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA16(YHR026W)|FD-Score:3.73|P-value:9.59E-5||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:YAP5(YIR018W)|FD-Score:-4.54|P-value:2.83E-6||SGD DESC:Basic leucine zipper (bZIP) iron-sensing transcription factor; YAP5 has a paralog, YAP7, that arose from the whole genome duplication Gene:YBL008W-A(YBL008W-A_p)|FD-Score:7.94|P-value:9.73E-16||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBR056W-A(YBR056W-A_p)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the dubious ORF YBR056C-B Gene:YCR064C(YCR064C_d)|FD-Score:5.52|P-value:1.72E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YEL028W(YEL028W_d)|FD-Score:4.4|P-value:5.31E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YFR012W-A(YFR012W-A_p)|FD-Score:4.78|P-value:8.88E-7||SGD DESC:Putative protein of unknown function; identified by homology Gene:YGR035W-A(YGR035W-A_p)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Putative protein of unknown function Gene:YGR064W(YGR064W_d)|FD-Score:3.17|P-value:7.54E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YGR168C(YGR168C_p)|FD-Score:3.16|P-value:8.00E-4||SGD DESC:Putative protein of unknown function; YGR168C is not an essential gene Gene:YIL055C(YIL055C_p)|FD-Score:3.17|P-value:7.75E-4||SGD DESC:Putative protein of unknown function Gene:YJL152W(YJL152W_d)|FD-Score:4.07|P-value:2.31E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR001C(YLR001C_p)|FD-Score:4.1|P-value:2.06E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; predicted to be palmitoylated Gene:YML057C-A(YML057C-A_d)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene CMP2/YML057W Gene:YML122C(YML122C_d)|FD-Score:5.07|P-value:1.99E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR245W(YMR245W_d)|FD-Score:3.88|P-value:5.28E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPK1(YKL126W)|FD-Score:5.62|P-value:9.57E-9||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPR170C(YPR170C_d)|FD-Score:-3.11|P-value:9.21E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B Gene:YPT7(YML001W)|FD-Score:3.95|P-value:3.89E-5||SGD DESC:Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YFR050C5.472.24E-81.20PRE4Beta 7 subunit of the 20S proteasome
YDR023W4.279.65E-60.29SES1Cytosolic seryl-tRNA synthetase, class II aminoacyl-tRNA synthetase that aminoacylates tRNA(Ser), displays tRNA-dependent amino acid recognition which enhances discrimination of the serine substrate, interacts with peroxin Pex21p
YGL099W3.983.45E-50.12LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YDR526C_d3.865.68E-50.03YDR526C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YBR234C3.836.35E-50.23ARC40Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
YPR143W3.601.58E-40.25RRP15Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs
YER036C3.353.97E-40.09ARB1ATPase of the ATP-binding cassette (ABC) family involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p
YDR280W3.275.42E-40.13RRP45Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress
YGR091W3.148.56E-40.13PRP31Splicing factor, component of the U4/U6-U5 snRNP complex
YGL169W3.010.001320.24SUA5Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family
YIL106W2.760.002860.22MOB1Component of the mitotic exit network; associates with and is required for the activation and Cdc15p-dependent phosphorylation of the Dbf2p kinase; required for cytokinesis and cell separation; component of the CCR4 transcriptional complex; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
YBR087W2.540.005520.05RFC5Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
YOR168W2.490.006430.17GLN4Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions
YNL038W2.320.010300.01GPI15Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-H protein
YPL169C2.310.010509.65E-4MEX67Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR202W18.201.14E-74ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
YBL008W-A_p7.949.73E-16YBL008W-A_pPutative protein of unknown function; identified by fungal homology and RT-PCR
YGL025C5.638.84E-9PGD1Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor
YKL126W5.629.57E-9YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YCR064C_d5.521.72E-8YCR064C_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31
YLR372W5.257.48E-8SUR4Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis
YKR093W5.091.82E-7PTR2Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p
YML122C_d5.071.99E-7YML122C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YFR012W-A_p4.788.88E-7YFR012W-A_pPutative protein of unknown function; identified by homology
YPL046C4.562.52E-6ELC1Elongin C, conserved among eukaryotes; forms a complex with Cul3p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; plays a role in global genomic repair
YEL028W_d4.405.31E-6YEL028W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YPL220W4.299.10E-6RPL1ARibosomal 60S subunit protein L1A; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1A has a paralog, RPL1B, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal
YNR023W4.231.15E-5SNF1273 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive
YJR117W4.181.47E-5STE24Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans
YKL190W4.141.71E-5CNB1Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress

GO enrichment analysis for SGTC_2820
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.3071.48E-129SGTC_21445628481 200.0 μMChembridge (Fragment library)28183990.130435ERG2
0.2944.30E-118SGTC_234nsc-17383 10.7 μMMiscellaneous4089740.109756ERG2
0.2791.00E-105SGTC_12440416-0015 52.5 μMChemDiv (Drug-like library)962010.0483871ERG2
0.2756.70E-103SGTC_7243910-0327 137.0 μMChemDiv (Drug-like library)28854830.157895ERG2
0.2702.45E-99SGTC_6341611-4317 11.2 μMChemDiv (Drug-like library)28435220.164384ERG2
0.2681.25E-97SGTC_2724maprotiline 69.6 μMMiscellaneous40110.0958904ERG2
0.2611.16E-92SGTC_1826st055655 41.6 μMTimTec (Natural product derivative library)6761680.168831ERG2
0.2581.73E-90SGTC_2720fluspirilene 36.9 μMMiscellaneous33960.0795455ERG2
0.2534.76E-87SGTC_5983937-0236 20.2 μMChemDiv (Drug-like library)13793130.2ERG2
0.2527.08E-86SGTC_12063978-0017 132.0 μMChemDiv (Drug-like library)38736770.114943ERG2
0.2512.36E-85SGTC_20555235184 200.0 μMChembridge (Fragment library)28360340.0434783ERG2
0.2493.10E-84SGTC_13601598-0022 39.8 μMChemDiv (Drug-like library)7615250.147059ERG2
0.2481.37E-83SGTC_8410251-0215 91.2 μMChemDiv (Drug-like library)38886140.0483871
0.2459.55E-82SGTC_28629027634 71.4 μMChembridge (Drug-like library)50125620.107143ERG2
0.2414.16E-79SGTC_1694st024011 44.9 μMTimTec (Natural product derivative library)27875330.137931ERG2

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_14780453-065635.7 μM0.5090915258496ChemDiv (Drug-like library)347.749762.81415
SGTC_10154130-452582 μM0.451363237ChemDiv (Drug-like library)392.834983.8032360S ribosome export
SGTC_507nimesulide162 μM0.44495Miscellaneous308.309782.19516
SGTC_8261683-5028118 μM0.396552722377ChemDiv (Drug-like library)261.703583.26212
SGTC_2787528454071.43 μM0.38983736958Chembridge (Drug-like library)291.729563.24613
SGTC_3176910658249.47 μM0.37704917018027Chembridge (Drug-like library)275.730163.74812
SGTC_2900532852885.29 μM0.372881795359Chembridge (Drug-like library)261.703583.26212Golgi
SGTC_3203911354349.47 μM0.36206917018012Chembridge (Drug-like library)241.28513.08412
SGTC_3285911281820.87 μM0.35714317017947Chembridge (Drug-like library)408.899043.29615cell wall
SGTC_3184910875849.47 μM0.355932902885Chembridge (Drug-like library)241.28512.94712