7994718

N-[3-chloro-2-(4-ethylpiperazin-1-yl)phenyl]-2-phenoxyacetamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2822
Screen concentration 71.4 μM
Source Chembridge (Drug-like library)
PubChem CID 1018011
SMILES CCN1CCN(CC1)C2=C(C=CC=C2Cl)NC(=O)COC3=CC=CC=C3
Standardized SMILES CCN1CCN(CC1)c2c(Cl)cccc2NC(=O)COc3ccccc3
Molecular weight 373.8765
ALogP 3.58
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.5
% growth inhibition (Hom. pool) -0.01


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1018011
Download HIP data (tab-delimited text)  (excel)
Gene:CAB2(YIL083C)|FD-Score:4.75|P-value:1.01E-6|Clearance:0.71||SGD DESC:Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity) Gene:CDC12(YHR107C)|FD-Score:3.75|P-value:8.99E-5|Clearance:0.3||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CDC20(YGL116W)|FD-Score:-3.43|P-value:3.07E-4|Clearance:0||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:GLN1(YPR035W)|FD-Score:5.24|P-value:8.10E-8|Clearance:0.71||SGD DESC:Glutamine synthetase (GS); synthesizes glutamine from glutamate and ammonia; with Glt1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source and by amino acid limitation; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:GLN4(YOR168W)|FD-Score:3.1|P-value:9.76E-4|Clearance:0.01||SGD DESC:Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions Gene:NSE3(YDR288W)|FD-Score:3.43|P-value:3.05E-4|Clearance:0.24||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress Gene:PAP1(YKR002W)|FD-Score:4.6|P-value:2.14E-6|Clearance:0.71||SGD DESC:Poly(A) polymerase, one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs; required for gene looping Gene:PRP22(YER013W)|FD-Score:5.28|P-value:6.40E-8|Clearance:0.71||SGD DESC:DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes Gene:RNA14(YMR061W)|FD-Score:4.78|P-value:8.89E-7|Clearance:0.71||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RPN5(YDL147W)|FD-Score:-4|P-value:3.17E-5|Clearance:0||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RSC58(YLR033W)|FD-Score:3.45|P-value:2.84E-4|Clearance:0.01||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SMC4(YLR086W)|FD-Score:-3.69|P-value:1.12E-4|Clearance:0||SGD DESC:Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate Gene:SPP382(YLR424W)|FD-Score:3.14|P-value:8.51E-4|Clearance:0.04||SGD DESC:Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation Gene:SRP102(YKL154W)|FD-Score:3.19|P-value:7.07E-4|Clearance:0.05||SGD DESC:Signal recognition particle (SRP) receptor beta subunit; involved in SRP-dependent protein targeting; anchors the alpha subunit, Srp101p to the ER membrane Gene:SSL1(YLR005W)|FD-Score:3.44|P-value:2.90E-4|Clearance:0.01||SGD DESC:Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p Gene:SWI1(YPL016W)|FD-Score:4.45|P-value:4.27E-6|Clearance:0.71||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer Gene:YKL153W(YKL153W_d)|FD-Score:5.38|P-value:3.74E-8|Clearance:0.71||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant Gene:CAB2(YIL083C)|FD-Score:4.75|P-value:1.01E-6|Clearance:0.71||SGD DESC:Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity) Gene:CDC12(YHR107C)|FD-Score:3.75|P-value:8.99E-5|Clearance:0.3||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CDC20(YGL116W)|FD-Score:-3.43|P-value:3.07E-4|Clearance:0||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:GLN1(YPR035W)|FD-Score:5.24|P-value:8.10E-8|Clearance:0.71||SGD DESC:Glutamine synthetase (GS); synthesizes glutamine from glutamate and ammonia; with Glt1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source and by amino acid limitation; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:GLN4(YOR168W)|FD-Score:3.1|P-value:9.76E-4|Clearance:0.01||SGD DESC:Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions Gene:NSE3(YDR288W)|FD-Score:3.43|P-value:3.05E-4|Clearance:0.24||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress Gene:PAP1(YKR002W)|FD-Score:4.6|P-value:2.14E-6|Clearance:0.71||SGD DESC:Poly(A) polymerase, one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs; required for gene looping Gene:PRP22(YER013W)|FD-Score:5.28|P-value:6.40E-8|Clearance:0.71||SGD DESC:DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes Gene:RNA14(YMR061W)|FD-Score:4.78|P-value:8.89E-7|Clearance:0.71||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RPN5(YDL147W)|FD-Score:-4|P-value:3.17E-5|Clearance:0||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RSC58(YLR033W)|FD-Score:3.45|P-value:2.84E-4|Clearance:0.01||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SMC4(YLR086W)|FD-Score:-3.69|P-value:1.12E-4|Clearance:0||SGD DESC:Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate Gene:SPP382(YLR424W)|FD-Score:3.14|P-value:8.51E-4|Clearance:0.04||SGD DESC:Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation Gene:SRP102(YKL154W)|FD-Score:3.19|P-value:7.07E-4|Clearance:0.05||SGD DESC:Signal recognition particle (SRP) receptor beta subunit; involved in SRP-dependent protein targeting; anchors the alpha subunit, Srp101p to the ER membrane Gene:SSL1(YLR005W)|FD-Score:3.44|P-value:2.90E-4|Clearance:0.01||SGD DESC:Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p Gene:SWI1(YPL016W)|FD-Score:4.45|P-value:4.27E-6|Clearance:0.71||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer Gene:YKL153W(YKL153W_d)|FD-Score:5.38|P-value:3.74E-8|Clearance:0.71||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1018011
Download HOP data (tab-delimited text)  (excel)
Gene:ATC1(YDR184C)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:Nuclear protein; possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; relative distribution to the nucleus decreases upon DNA replication stress Gene:BUD19(YJL188C_d)|FD-Score:3.1|P-value:9.81E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CNM67(YNL225C)|FD-Score:8.2|P-value:1.23E-16||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:COS6(YGR295C)|FD-Score:-3.23|P-value:6.10E-4||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:CPR3(YML078W)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Mitochondrial peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; involved in protein refolding after import into mitochondria Gene:DCI1(YOR180C)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Peroxisomal protein; identification as a delta(3,5)-delta(2,4)-dienoyl-CoA isomerase involved in fatty acid metabolism is disputed Gene:DDI3(YNL335W_p)|FD-Score:4.2|P-value:1.36E-5||SGD DESC:Protein of unknown function; expression is induced over 100-fold by DNA damage; induction decreased in rad6 and rad18 mutants Gene:GZF3(YJL110C)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication Gene:HAA1(YPR008W)|FD-Score:3.82|P-value:6.67E-5||SGD DESC:Transcriptional activator involved in adaptation to weak acid stress; activates transcription of TPO2, YRO2, and other genes encoding membrane stress proteins; HAA1 has a paralog, CUP2, that arose from the whole genome duplication; relocalizes from cytoplasm to nucleus upon DNA replication stress Gene:HAT2(YEL056W)|FD-Score:3.89|P-value:5.10E-5||SGD DESC:Subunit of the Hat1p-Hat2p histone acetyltransferase complex; required for high affinity binding of the complex to free histone H4, thereby enhancing Hat1p activity; similar to human RbAp46 and 48; has a role in telomeric silencing Gene:HIS5(YIL116W)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Histidinol-phosphate aminotransferase, catalyzes the seventh step in histidine biosynthesis; responsive to general control of amino acid biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts Gene:IDP2(YLR174W)|FD-Score:-4.64|P-value:1.72E-6||SGD DESC:Cytosolic NADP-specific isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate; levels are elevated during growth on non-fermentable carbon sources and reduced during growth on glucose; IDP2 has a paralog, IDP3, that arose from the whole genome duplication Gene:IMG1(YCR046C)|FD-Score:-3.63|P-value:1.44E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, required for respiration and for maintenance of the mitochondrial genome Gene:IRC4(YDR540C)|FD-Score:3.46|P-value:2.73E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:KSP1(YHR082C)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Serine/threonine protein kinase; associates with TORC1 and likely involved in TOR signaling cascades; negative regulator of autophagy; nuclear translocation required for haploid filamentous growth; regulates filamentous growth induced nuclear translocation of Bcy1p, Fus3p, and Sks1p; overproduction causes allele-specific suppression of prp20-10; protein abundance increases in response to DNA replication stress Gene:LAS21(YJL062W)|FD-Score:-3.55|P-value:1.95E-4||SGD DESC:Integral plasma membrane protein involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity Gene:LHS1(YKL073W)|FD-Score:6.15|P-value:3.78E-10||SGD DESC:Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway Gene:MKK2(YPL140C)|FD-Score:7.94|P-value:1.02E-15||SGD DESC:MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk1p; MKK2 has a paralog, MKK1, that arose from the whole genome duplication Gene:MLP2(YIL149C)|FD-Score:-3.11|P-value:9.31E-4||SGD DESC:Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length Gene:MRPS9(YBR146W)|FD-Score:3.79|P-value:7.64E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MYO4(YAL029C)|FD-Score:-3.18|P-value:7.35E-4||SGD DESC:Type V myosin motor involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p; MYO4 has a paralog, MYO2, that arose from the whole genome duplication Gene:NEJ1(YLR265C)|FD-Score:3.82|P-value:6.75E-5||SGD DESC:Protein involved in regulation of nonhomologous end joining; interacts with DNA ligase IV components Dnl4p and Lif1p; repressed by MAT heterozygosity; regulates cellular distribution of Lif1p Gene:OTU1(YFL044C)|FD-Score:-3.21|P-value:6.61E-4||SGD DESC:Deubiquitylation enzyme that binds to the chaperone-ATPase Cdc48p; may contribute to regulation of protein degradation by deubiquitylating substrates that have been ubiquitylated by Ufd2p; member of the Ovarian Tumor (OTU) family; protein abundance increases in response to DNA replication stress Gene:PCK1(YKR097W)|FD-Score:4.96|P-value:3.47E-7||SGD DESC:Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol Gene:PDR11(YIL013C)|FD-Score:3.35|P-value:4.02E-4||SGD DESC:ATP-binding cassette (ABC) transporter, multidrug transporter involved in multiple drug resistance; mediates sterol uptake when sterol biosynthesis is compromised; regulated by Pdr1p; required for anaerobic growth Gene:PTK1(YKL198C)|FD-Score:3.17|P-value:7.49E-4||SGD DESC:Putative serine/threonine protein kinase that regulates spermine uptake; involved in polyamine transport; possible mitochondrial protein Gene:RDS2(YPL133C)|FD-Score:3.91|P-value:4.59E-5||SGD DESC:Transcription factor involved in regulating gluconeogenesis and glyoxylate cycle genes; member of the zinc cluster family of proteins; confers resistance to ketoconazole Gene:RHO4(YKR055W)|FD-Score:3.79|P-value:7.48E-5||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely to be involved in the establishment of cell polarity Gene:RPS29B(YDL061C)|FD-Score:4.96|P-value:3.51E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29B has a paralog, RPS29A, that arose from the whole genome duplication Gene:SEC22(YLR268W)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SRB8(YCR081W)|FD-Score:-3.87|P-value:5.54E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:STD1(YOR047C)|FD-Score:-4.69|P-value:1.35E-6||SGD DESC:Protein involved in control of glucose-regulated gene expression; interacts with kinase Snf1p, glucose sensors Snf3p and Rgt2p, TATA-binding Spt15p; regulator of transcription factor Rgt1p; interactions with Pma1p appear to propagate [GAR+] Gene:STE13(YOR219C)|FD-Score:3.35|P-value:4.06E-4||SGD DESC:Dipeptidyl aminopeptidase, Golgi integral membrane protein that cleaves on the carboxyl side of repeating -X-Ala- sequences, required for maturation of alpha factor, transcription is induced by a-factor Gene:STE24(YJR117W)|FD-Score:-3.11|P-value:9.25E-4||SGD DESC:Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans Gene:SWR1(YDR334W)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:THI4(YGR144W)|FD-Score:4.31|P-value:8.03E-6||SGD DESC:Thiazole synthase, abundant protein involved in the formation of the thiazole moiety of thiamine during thiamine biosynthesis; acts more as a co-substrate rather than an enzyme by providing the sulphur source for thiazole formation; undergoes a single turnover only; required for mitochondrial genome stability in response to DNA damaging agents Gene:TIF4631(YGR162W)|FD-Score:-4.24|P-value:1.11E-5||SGD DESC:Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication Gene:TMA46(YOR091W)|FD-Score:-4.01|P-value:3.04E-5||SGD DESC:Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p Gene:TOD6(YBL054W)|FD-Score:4.43|P-value:4.76E-6||SGD DESC:PAC motif binding protein involved in rRNA and ribosome biogenesis; subunit of the RPD3L histone deacetylase complex; Myb-like HTH transcription factor; hypophosphorylated by rapamycin treatment in a Sch9p-dependent manner; TOD6 has a paralog, DOT6, that arose from the whole genome duplication Gene:TRP2(YER090W)|FD-Score:5.36|P-value:4.05E-8||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TUS1(YLR425W)|FD-Score:-3.82|P-value:6.74E-5||SGD DESC:Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate Gene:UBC4(YBR082C)|FD-Score:-4.12|P-value:1.87E-5||SGD DESC:Ubiquitin-conjugating enzyme (E2); mediates degradation of abnormal or excess proteins, including calmodulin and histone H3; interacts with many SCF ubiquitin protein ligases; component of the cellular stress response; UBC4 has a paralog, UBC5, that arose from the whole genome duplication Gene:URM1(YIL008W)|FD-Score:5.39|P-value:3.54E-8||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:YAP1801(YHR161C)|FD-Score:3.97|P-value:3.56E-5||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1801 has a paralog, YAP1802, that arose from the whole genome duplication Gene:YCK3(YER123W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway Gene:YDL041W(YDL041W_d)|FD-Score:3.14|P-value:8.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YDR003W-A(YDR003W-A_p)|FD-Score:4.79|P-value:8.32E-7||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YDR271C(YDR271C_d)|FD-Score:-3.48|P-value:2.49E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W Gene:YJL152W(YJL152W_d)|FD-Score:-5.27|P-value:6.75E-8||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL091C(YKL091C)|FD-Score:4.14|P-value:1.71E-5||SGD DESC:Putative homolog of Sec14p, which is a phosphatidylinositol/phosphatidylcholine transfer protein involved in lipid metabolism; localizes to the nucleus; contains a CRAL/TRIO domain and binds several lipids in a large-scale study Gene:YLR346C(YLR346C_p)|FD-Score:-4.56|P-value:2.51E-6||SGD DESC:Putative protein of unknown function found in mitochondria; expression is regulated by transcription factors involved in pleiotropic drug resistance, Pdr1p and Yrr1p; YLR346C is not an essential gene Gene:YNL144C(YNL144C_p)|FD-Score:3.9|P-value:4.84E-5||SGD DESC:Putative protein of unknown function; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; contains a PH domain and binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study Gene:YNR005C(YNR005C_d)|FD-Score:3.31|P-value:4.74E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL114C(YOL114C_p)|FD-Score:3.79|P-value:7.68E-5||SGD DESC:Putative protein of unknown function with similarity to human ICT1 and prokaryotic factors that may function in translation termination; YOL114C is not an essential gene Gene:YOL118C(YOL118C_d)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOX1(YML027W)|FD-Score:-3.74|P-value:9.31E-5||SGD DESC:Homeobox transcriptional repressor; binds to Mcm1p and to early cell cycle boxes (ECBs) in the promoters of cell cycle-regulated genes expressed in M/G1 phase; expression is cell cycle-regulated; potential Cdc28p substrate; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOX1 has a paralog, YHP1, that arose from the whole genome duplication Gene:YPL191C(YPL191C_p)|FD-Score:6.51|P-value:3.69E-11||SGD DESC:Putative protein of unknown function; diploid deletion strain exhibits high budding index; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YPR170C(YPR170C_d)|FD-Score:4.02|P-value:2.95E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B Gene:YPR172W(YPR172W_p)|FD-Score:6.75|P-value:7.60E-12||SGD DESC:Putative pyridoxal 5'-phosphate synthase; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; YPR172W has a paralog, YLR456W, that arose from the whole genome duplication Gene:ZPS1(YOL154W)|FD-Score:-3.5|P-value:2.34E-4||SGD DESC:Putative GPI-anchored protein; transcription is induced under low-zinc conditions, as mediated by the Zap1p transcription factor, and at alkaline pH Gene:ATC1(YDR184C)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:Nuclear protein; possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; relative distribution to the nucleus decreases upon DNA replication stress Gene:BUD19(YJL188C_d)|FD-Score:3.1|P-value:9.81E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CNM67(YNL225C)|FD-Score:8.2|P-value:1.23E-16||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:COS6(YGR295C)|FD-Score:-3.23|P-value:6.10E-4||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:CPR3(YML078W)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Mitochondrial peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; involved in protein refolding after import into mitochondria Gene:DCI1(YOR180C)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Peroxisomal protein; identification as a delta(3,5)-delta(2,4)-dienoyl-CoA isomerase involved in fatty acid metabolism is disputed Gene:DDI3(YNL335W_p)|FD-Score:4.2|P-value:1.36E-5||SGD DESC:Protein of unknown function; expression is induced over 100-fold by DNA damage; induction decreased in rad6 and rad18 mutants Gene:GZF3(YJL110C)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication Gene:HAA1(YPR008W)|FD-Score:3.82|P-value:6.67E-5||SGD DESC:Transcriptional activator involved in adaptation to weak acid stress; activates transcription of TPO2, YRO2, and other genes encoding membrane stress proteins; HAA1 has a paralog, CUP2, that arose from the whole genome duplication; relocalizes from cytoplasm to nucleus upon DNA replication stress Gene:HAT2(YEL056W)|FD-Score:3.89|P-value:5.10E-5||SGD DESC:Subunit of the Hat1p-Hat2p histone acetyltransferase complex; required for high affinity binding of the complex to free histone H4, thereby enhancing Hat1p activity; similar to human RbAp46 and 48; has a role in telomeric silencing Gene:HIS5(YIL116W)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Histidinol-phosphate aminotransferase, catalyzes the seventh step in histidine biosynthesis; responsive to general control of amino acid biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts Gene:IDP2(YLR174W)|FD-Score:-4.64|P-value:1.72E-6||SGD DESC:Cytosolic NADP-specific isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate; levels are elevated during growth on non-fermentable carbon sources and reduced during growth on glucose; IDP2 has a paralog, IDP3, that arose from the whole genome duplication Gene:IMG1(YCR046C)|FD-Score:-3.63|P-value:1.44E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, required for respiration and for maintenance of the mitochondrial genome Gene:IRC4(YDR540C)|FD-Score:3.46|P-value:2.73E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:KSP1(YHR082C)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Serine/threonine protein kinase; associates with TORC1 and likely involved in TOR signaling cascades; negative regulator of autophagy; nuclear translocation required for haploid filamentous growth; regulates filamentous growth induced nuclear translocation of Bcy1p, Fus3p, and Sks1p; overproduction causes allele-specific suppression of prp20-10; protein abundance increases in response to DNA replication stress Gene:LAS21(YJL062W)|FD-Score:-3.55|P-value:1.95E-4||SGD DESC:Integral plasma membrane protein involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity Gene:LHS1(YKL073W)|FD-Score:6.15|P-value:3.78E-10||SGD DESC:Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway Gene:MKK2(YPL140C)|FD-Score:7.94|P-value:1.02E-15||SGD DESC:MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk1p; MKK2 has a paralog, MKK1, that arose from the whole genome duplication Gene:MLP2(YIL149C)|FD-Score:-3.11|P-value:9.31E-4||SGD DESC:Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length Gene:MRPS9(YBR146W)|FD-Score:3.79|P-value:7.64E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MYO4(YAL029C)|FD-Score:-3.18|P-value:7.35E-4||SGD DESC:Type V myosin motor involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p; MYO4 has a paralog, MYO2, that arose from the whole genome duplication Gene:NEJ1(YLR265C)|FD-Score:3.82|P-value:6.75E-5||SGD DESC:Protein involved in regulation of nonhomologous end joining; interacts with DNA ligase IV components Dnl4p and Lif1p; repressed by MAT heterozygosity; regulates cellular distribution of Lif1p Gene:OTU1(YFL044C)|FD-Score:-3.21|P-value:6.61E-4||SGD DESC:Deubiquitylation enzyme that binds to the chaperone-ATPase Cdc48p; may contribute to regulation of protein degradation by deubiquitylating substrates that have been ubiquitylated by Ufd2p; member of the Ovarian Tumor (OTU) family; protein abundance increases in response to DNA replication stress Gene:PCK1(YKR097W)|FD-Score:4.96|P-value:3.47E-7||SGD DESC:Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol Gene:PDR11(YIL013C)|FD-Score:3.35|P-value:4.02E-4||SGD DESC:ATP-binding cassette (ABC) transporter, multidrug transporter involved in multiple drug resistance; mediates sterol uptake when sterol biosynthesis is compromised; regulated by Pdr1p; required for anaerobic growth Gene:PTK1(YKL198C)|FD-Score:3.17|P-value:7.49E-4||SGD DESC:Putative serine/threonine protein kinase that regulates spermine uptake; involved in polyamine transport; possible mitochondrial protein Gene:RDS2(YPL133C)|FD-Score:3.91|P-value:4.59E-5||SGD DESC:Transcription factor involved in regulating gluconeogenesis and glyoxylate cycle genes; member of the zinc cluster family of proteins; confers resistance to ketoconazole Gene:RHO4(YKR055W)|FD-Score:3.79|P-value:7.48E-5||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely to be involved in the establishment of cell polarity Gene:RPS29B(YDL061C)|FD-Score:4.96|P-value:3.51E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29B has a paralog, RPS29A, that arose from the whole genome duplication Gene:SEC22(YLR268W)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SRB8(YCR081W)|FD-Score:-3.87|P-value:5.54E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:STD1(YOR047C)|FD-Score:-4.69|P-value:1.35E-6||SGD DESC:Protein involved in control of glucose-regulated gene expression; interacts with kinase Snf1p, glucose sensors Snf3p and Rgt2p, TATA-binding Spt15p; regulator of transcription factor Rgt1p; interactions with Pma1p appear to propagate [GAR+] Gene:STE13(YOR219C)|FD-Score:3.35|P-value:4.06E-4||SGD DESC:Dipeptidyl aminopeptidase, Golgi integral membrane protein that cleaves on the carboxyl side of repeating -X-Ala- sequences, required for maturation of alpha factor, transcription is induced by a-factor Gene:STE24(YJR117W)|FD-Score:-3.11|P-value:9.25E-4||SGD DESC:Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans Gene:SWR1(YDR334W)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:THI4(YGR144W)|FD-Score:4.31|P-value:8.03E-6||SGD DESC:Thiazole synthase, abundant protein involved in the formation of the thiazole moiety of thiamine during thiamine biosynthesis; acts more as a co-substrate rather than an enzyme by providing the sulphur source for thiazole formation; undergoes a single turnover only; required for mitochondrial genome stability in response to DNA damaging agents Gene:TIF4631(YGR162W)|FD-Score:-4.24|P-value:1.11E-5||SGD DESC:Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication Gene:TMA46(YOR091W)|FD-Score:-4.01|P-value:3.04E-5||SGD DESC:Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p Gene:TOD6(YBL054W)|FD-Score:4.43|P-value:4.76E-6||SGD DESC:PAC motif binding protein involved in rRNA and ribosome biogenesis; subunit of the RPD3L histone deacetylase complex; Myb-like HTH transcription factor; hypophosphorylated by rapamycin treatment in a Sch9p-dependent manner; TOD6 has a paralog, DOT6, that arose from the whole genome duplication Gene:TRP2(YER090W)|FD-Score:5.36|P-value:4.05E-8||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TUS1(YLR425W)|FD-Score:-3.82|P-value:6.74E-5||SGD DESC:Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate Gene:UBC4(YBR082C)|FD-Score:-4.12|P-value:1.87E-5||SGD DESC:Ubiquitin-conjugating enzyme (E2); mediates degradation of abnormal or excess proteins, including calmodulin and histone H3; interacts with many SCF ubiquitin protein ligases; component of the cellular stress response; UBC4 has a paralog, UBC5, that arose from the whole genome duplication Gene:URM1(YIL008W)|FD-Score:5.39|P-value:3.54E-8||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:YAP1801(YHR161C)|FD-Score:3.97|P-value:3.56E-5||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1801 has a paralog, YAP1802, that arose from the whole genome duplication Gene:YCK3(YER123W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway Gene:YDL041W(YDL041W_d)|FD-Score:3.14|P-value:8.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YDR003W-A(YDR003W-A_p)|FD-Score:4.79|P-value:8.32E-7||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YDR271C(YDR271C_d)|FD-Score:-3.48|P-value:2.49E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W Gene:YJL152W(YJL152W_d)|FD-Score:-5.27|P-value:6.75E-8||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL091C(YKL091C)|FD-Score:4.14|P-value:1.71E-5||SGD DESC:Putative homolog of Sec14p, which is a phosphatidylinositol/phosphatidylcholine transfer protein involved in lipid metabolism; localizes to the nucleus; contains a CRAL/TRIO domain and binds several lipids in a large-scale study Gene:YLR346C(YLR346C_p)|FD-Score:-4.56|P-value:2.51E-6||SGD DESC:Putative protein of unknown function found in mitochondria; expression is regulated by transcription factors involved in pleiotropic drug resistance, Pdr1p and Yrr1p; YLR346C is not an essential gene Gene:YNL144C(YNL144C_p)|FD-Score:3.9|P-value:4.84E-5||SGD DESC:Putative protein of unknown function; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; contains a PH domain and binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study Gene:YNR005C(YNR005C_d)|FD-Score:3.31|P-value:4.74E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL114C(YOL114C_p)|FD-Score:3.79|P-value:7.68E-5||SGD DESC:Putative protein of unknown function with similarity to human ICT1 and prokaryotic factors that may function in translation termination; YOL114C is not an essential gene Gene:YOL118C(YOL118C_d)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOX1(YML027W)|FD-Score:-3.74|P-value:9.31E-5||SGD DESC:Homeobox transcriptional repressor; binds to Mcm1p and to early cell cycle boxes (ECBs) in the promoters of cell cycle-regulated genes expressed in M/G1 phase; expression is cell cycle-regulated; potential Cdc28p substrate; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOX1 has a paralog, YHP1, that arose from the whole genome duplication Gene:YPL191C(YPL191C_p)|FD-Score:6.51|P-value:3.69E-11||SGD DESC:Putative protein of unknown function; diploid deletion strain exhibits high budding index; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YPR170C(YPR170C_d)|FD-Score:4.02|P-value:2.95E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B Gene:YPR172W(YPR172W_p)|FD-Score:6.75|P-value:7.60E-12||SGD DESC:Putative pyridoxal 5'-phosphate synthase; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; YPR172W has a paralog, YLR456W, that arose from the whole genome duplication Gene:ZPS1(YOL154W)|FD-Score:-3.5|P-value:2.34E-4||SGD DESC:Putative GPI-anchored protein; transcription is induced under low-zinc conditions, as mediated by the Zap1p transcription factor, and at alkaline pH

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YKL153W_d5.383.74E-80.70YKL153W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant
YER013W5.286.40E-80.70PRP22DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes
YPR035W5.248.10E-80.70GLN1Glutamine synthetase (GS); synthesizes glutamine from glutamate and ammonia; with Glt1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source and by amino acid limitation; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YMR061W4.788.89E-70.70RNA14Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping
YIL083C4.751.01E-60.70CAB2Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity)
YKR002W4.602.14E-60.70PAP1Poly(A) polymerase, one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs; required for gene looping
YPL016W4.454.27E-60.70SWI1Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer
YHR107C3.758.99E-50.30CDC12Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YLR033W3.452.84E-40.01RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YLR005W3.442.90E-40.01SSL1Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p
YDR288W3.433.05E-40.24NSE3Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress
YKL154W3.197.07E-40.05SRP102Signal recognition particle (SRP) receptor beta subunit; involved in SRP-dependent protein targeting; anchors the alpha subunit, Srp101p to the ER membrane
YLR424W3.148.51E-40.04SPP382Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation
YOR168W3.109.76E-40.01GLN4Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions
YMR033W3.090.001000.05ARP9Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YNL225C8.201.23E-16CNM67Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication
YPL140C7.941.02E-15MKK2MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk1p; MKK2 has a paralog, MKK1, that arose from the whole genome duplication
YPR172W_p6.757.60E-12YPR172W_pPutative pyridoxal 5'-phosphate synthase; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; YPR172W has a paralog, YLR456W, that arose from the whole genome duplication
YPL191C_p6.513.69E-11YPL191C_pPutative protein of unknown function; diploid deletion strain exhibits high budding index; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
YKL073W6.153.78E-10LHS1Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway
YIL008W5.393.54E-8URM1Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response
YER090W5.364.05E-8TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YKR097W4.963.47E-7PCK1Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol
YDL061C4.963.51E-7RPS29BProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29B has a paralog, RPS29A, that arose from the whole genome duplication
YDR003W-A_p4.798.32E-7YDR003W-A_pPutative protein of unknown function; identified by expression profiling and mass spectrometry
YBL054W4.434.76E-6TOD6PAC motif binding protein involved in rRNA and ribosome biogenesis; subunit of the RPD3L histone deacetylase complex; Myb-like HTH transcription factor; hypophosphorylated by rapamycin treatment in a Sch9p-dependent manner; TOD6 has a paralog, DOT6, that arose from the whole genome duplication
YGR144W4.318.03E-6THI4Thiazole synthase, abundant protein involved in the formation of the thiazole moiety of thiamine during thiamine biosynthesis; acts more as a co-substrate rather than an enzyme by providing the sulphur source for thiazole formation; undergoes a single turnover only; required for mitochondrial genome stability in response to DNA damaging agents
YNL335W_p4.201.36E-5DDI3_pProtein of unknown function; expression is induced over 100-fold by DNA damage; induction decreased in rad6 and rad18 mutants
YKL091C4.141.71E-5YKL091CPutative homolog of Sec14p, which is a phosphatidylinositol/phosphatidylcholine transfer protein involved in lipid metabolism; localizes to the nucleus; contains a CRAL/TRIO domain and binds several lipids in a large-scale study
YPR170C_d4.022.95E-5YPR170C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B

GO enrichment analysis for SGTC_2822
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0992.86E-14SGTC_28247998404 52.0 μMChembridge (Drug-like library)8378510.157143
0.0713.92E-8SGTC_13551499-0035 1.7 μMChemDiv (Drug-like library)15485380.136364
0.0714.71E-8SGTC_28187996756 13.0 μMChembridge (Drug-like library)22416210.146341
0.0691.09E-7SGTC_22246650123 117.6 μMChembridge (Fragment library)29083700.113924
0.0672.48E-7SGTC_11564092-0367 4.9 μMChemDiv (Drug-like library)13575850.202703
0.0655.43E-7SGTC_28177995342 71.4 μMChembridge (Drug-like library)188799750.219178
0.0612.73E-6SGTC_30829117008 49.5 μMChembridge (Drug-like library)414485290.169014
0.0604.50E-6SGTC_13241418-0026 34.4 μMChemDiv (Drug-like library)13725130.0657895
0.0595.41E-6SGTC_1457k015-0032 57.3 μMChemDiv (Drug-like library)43026600.15
0.0586.99E-6SGTC_14554262-0120 131.0 μMChemDiv (Drug-like library)15295870.183333plasma membrane duress
0.0588.73E-6SGTC_22107115885 190.4 μMChembridge (Fragment library)29270390.0588235cell wall signaling
0.0571.00E-5SGTC_27867743617 45.5 μMChembridge (Drug-like library)5836740.111111
0.0561.55E-5SGTC_29849022109 39.0 μMChembridge (Drug-like library)64592070.231884
0.0561.85E-5SGTC_22037227154 166.4 μMChembridge (Fragment library)8963040.151515
0.0552.24E-5SGTC_2520obtusaquinone 4.7 μMMicrosource (Natural product library)59678720.121622

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1300136-0219337.66 μM0.509434234244ChemDiv (Drug-like library)296.148643.77812
SGTC_3114912276649.47 μM0.437517176985Chembridge (Drug-like library)375.8244632.86314
SGTC_3066911724549.47 μM0.42857117176980Chembridge (Drug-like library)371.860583.14413
SGTC_2895905444758.44 μM0.4237292697450Chembridge (Drug-like library)343.850483.16113
SGTC_22446635614200 μM0.418182836499Chembridge (Fragment library)234.079262.01512
SGTC_74000s-0487160.28 μM0.413793677548ChemDiv (Drug-like library)277.702982.87223
SGTC_3074911601849.47 μM0.40909117027303Chembridge (Drug-like library)385.887163.6313RNA processing & uracil transport
SGTC_3048909315549.47 μM0.39062525236002Chembridge (Drug-like library)318.45372.73313
SGTC_2994905352071.43 μM0.3709684647384Chembridge (Drug-like library)300.782683.27912RPP1 & pyrimidine depletion
SGTC_3202911376149.47 μM0.3472224798415Chembridge (Drug-like library)377.8403433.43915