7998404

3-methyl-N-(3-methylsulfanylphenyl)benzamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2824
Screen concentration 52.0 μM
Source Chembridge (Drug-like library)
PubChem CID 837851
SMILES CC1=CC=CC(=C1)C(=O)NC2=CC(=CC=C2)SC
Standardized SMILES CSc1cccc(NC(=O)c2cccc(C)c2)c1
Molecular weight 257.3507
ALogP 3.64
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 21.21
% growth inhibition (Hom. pool) 14.98


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 837851
Download HIP data (tab-delimited text)  (excel)
Gene:ALG2(YGL065C)|FD-Score:4.62|P-value:1.90E-6|Clearance:1.14||SGD DESC:Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol Gene:ARH1(YDR376W)|FD-Score:3.25|P-value:5.72E-4|Clearance:0.08||SGD DESC:Oxidoreductase of the mitochondrial inner membrane, involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viability Gene:ARP9(YMR033W)|FD-Score:-4.11|P-value:1.95E-5|Clearance:0||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:BCP1(YDR361C)|FD-Score:5.25|P-value:7.69E-8|Clearance:0.63||SGD DESC:Essential protein involved in nuclear export of Mss4p, which is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport Gene:CDC21(YOR074C)|FD-Score:3.14|P-value:8.54E-4|Clearance:0.04||SGD DESC:Thymidylate synthase, required for de novo biosynthesis of pyrimidine deoxyribonucleotides; expression is induced at G1/S Gene:DML1(YMR211W)|FD-Score:3.42|P-value:3.08E-4|Clearance:0.1||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:ERG12(YMR208W)|FD-Score:-4.08|P-value:2.22E-5|Clearance:0||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:FRQ1(YDR373W)|FD-Score:3.1|P-value:9.74E-4|Clearance:0.02||SGD DESC:N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily Gene:GCD10(YNL062C)|FD-Score:3.17|P-value:7.63E-4|Clearance:0.03||SGD DESC:Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression Gene:PDC2(YDR081C)|FD-Score:3.32|P-value:4.46E-4|Clearance:0.07||SGD DESC:Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions Gene:RPA43(YOR340C)|FD-Score:3.49|P-value:2.46E-4|Clearance:0.06||SGD DESC:RNA polymerase I subunit A43 Gene:RPN5(YDL147W)|FD-Score:-7.36|P-value:8.96E-14|Clearance:0||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:SEC31(YDL195W)|FD-Score:5.42|P-value:2.90E-8|Clearance:0.18||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SNU114(YKL173W)|FD-Score:5.81|P-value:3.11E-9|Clearance:0.39||SGD DESC:GTPase component of U5 snRNP involved in mRNA splicing via spliceosome; binds directly to U5 snRNA; proposed to be involved in conformational changes of the spliceosome; similarity to ribosomal translocation factor EF-2 Gene:SWI1(YPL016W)|FD-Score:8.67|P-value:2.23E-18|Clearance:1.87||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer Gene:TOA1(YOR194C)|FD-Score:-5.13|P-value:1.45E-7|Clearance:0||SGD DESC:TFIIA large subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to largest and second largest subunits of human and Drosophila TFIIA Gene:YDR526C(YDR526C_d)|FD-Score:7.68|P-value:7.81E-15|Clearance:1.87||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ALG2(YGL065C)|FD-Score:4.62|P-value:1.90E-6|Clearance:1.14||SGD DESC:Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol Gene:ARH1(YDR376W)|FD-Score:3.25|P-value:5.72E-4|Clearance:0.08||SGD DESC:Oxidoreductase of the mitochondrial inner membrane, involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viability Gene:ARP9(YMR033W)|FD-Score:-4.11|P-value:1.95E-5|Clearance:0||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:BCP1(YDR361C)|FD-Score:5.25|P-value:7.69E-8|Clearance:0.63||SGD DESC:Essential protein involved in nuclear export of Mss4p, which is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport Gene:CDC21(YOR074C)|FD-Score:3.14|P-value:8.54E-4|Clearance:0.04||SGD DESC:Thymidylate synthase, required for de novo biosynthesis of pyrimidine deoxyribonucleotides; expression is induced at G1/S Gene:DML1(YMR211W)|FD-Score:3.42|P-value:3.08E-4|Clearance:0.1||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:ERG12(YMR208W)|FD-Score:-4.08|P-value:2.22E-5|Clearance:0||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:FRQ1(YDR373W)|FD-Score:3.1|P-value:9.74E-4|Clearance:0.02||SGD DESC:N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily Gene:GCD10(YNL062C)|FD-Score:3.17|P-value:7.63E-4|Clearance:0.03||SGD DESC:Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression Gene:PDC2(YDR081C)|FD-Score:3.32|P-value:4.46E-4|Clearance:0.07||SGD DESC:Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions Gene:RPA43(YOR340C)|FD-Score:3.49|P-value:2.46E-4|Clearance:0.06||SGD DESC:RNA polymerase I subunit A43 Gene:RPN5(YDL147W)|FD-Score:-7.36|P-value:8.96E-14|Clearance:0||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:SEC31(YDL195W)|FD-Score:5.42|P-value:2.90E-8|Clearance:0.18||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SNU114(YKL173W)|FD-Score:5.81|P-value:3.11E-9|Clearance:0.39||SGD DESC:GTPase component of U5 snRNP involved in mRNA splicing via spliceosome; binds directly to U5 snRNA; proposed to be involved in conformational changes of the spliceosome; similarity to ribosomal translocation factor EF-2 Gene:SWI1(YPL016W)|FD-Score:8.67|P-value:2.23E-18|Clearance:1.87||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer Gene:TOA1(YOR194C)|FD-Score:-5.13|P-value:1.45E-7|Clearance:0||SGD DESC:TFIIA large subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to largest and second largest subunits of human and Drosophila TFIIA Gene:YDR526C(YDR526C_d)|FD-Score:7.68|P-value:7.81E-15|Clearance:1.87||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 837851
Download HOP data (tab-delimited text)  (excel)
Gene:AAD4(YDL243C)|FD-Score:4.65|P-value:1.66E-6||SGD DESC:Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin Gene:ALF1(YNL148C)|FD-Score:-3.13|P-value:8.77E-4||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:APL6(YGR261C)|FD-Score:3.41|P-value:3.26E-4||SGD DESC:Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools Gene:BEM4(YPL161C)|FD-Score:5.7|P-value:5.89E-9||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BLI1(YKL061W_p)|FD-Score:4.9|P-value:4.86E-7||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome Gene:BNI1(YNL271C)|FD-Score:-3.4|P-value:3.40E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:CAR2(YLR438W)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:L-ornithine transaminase (OTAse); catalyzes the second step of arginine degradation, expression is dually-regulated by allophanate induction and a specific arginine induction process; not nitrogen catabolite repression sensitive; protein abundance increases in response to DNA replication stress Gene:CBP6(YBR120C)|FD-Score:5.11|P-value:1.65E-7||SGD DESC:Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex Gene:COS6(YGR295C)|FD-Score:-3.11|P-value:9.45E-4||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:CSG2(YBR036C)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:CSM1(YCR086W)|FD-Score:4.79|P-value:8.27E-7||SGD DESC:Nucleolar protein that forms a complex with Lrs4p and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:CWC15(YDR163W)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Non-essential protein involved in pre-mRNA splicing, component of a complex containing Cef1p; has similarity to S. pombe Cwf15p Gene:CYB5(YNL111C)|FD-Score:-3.45|P-value:2.76E-4||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:DIA4(YHR011W)|FD-Score:-3.34|P-value:4.23E-4||SGD DESC:Probable mitochondrial seryl-tRNA synthetase, mutant displays increased invasive and pseudohyphal growth Gene:DIN7(YDR263C)|FD-Score:6.11|P-value:4.94E-10||SGD DESC:Mitochondrial nuclease functioning in DNA repair and replication, modulates the stability of the mitochondrial genome, induced by exposure to mutagens, also induced during meiosis at a time nearly coincident with commitment to recombination Gene:DMA1(YHR115C)|FD-Score:-3.38|P-value:3.63E-4||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma2p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8 protein, similar to human Chfr; contains FHA and RING finger Gene:FAR3(YMR052W)|FD-Score:6.14|P-value:4.09E-10||SGD DESC:Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:FMO1(YHR176W)|FD-Score:4.53|P-value:2.99E-6||SGD DESC:Flavin-containing monooxygenase, localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins Gene:FMP40(YPL222W_p)|FD-Score:3.2|P-value:6.96E-4||SGD DESC:Putative protein of unknown function; proposed to be involved in responding to environmental stresses; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GCN2(YDR283C)|FD-Score:-3.35|P-value:4.11E-4||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCV1(YDR019C)|FD-Score:5.24|P-value:7.86E-8||SGD DESC:T subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm Gene:GEF1(YJR040W)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism Gene:GEP7(YGL057C)|FD-Score:4.42|P-value:4.98E-6||SGD DESC:Protein of unknown function; null mutant exhibits a respiratory growth defect and synthetic interactions with prohibitin (phb1) and gem1; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:HBT1(YDL223C)|FD-Score:4.28|P-value:9.29E-6||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:HOM6(YJR139C)|FD-Score:-3.62|P-value:1.47E-4||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:HOP1(YIL072W)|FD-Score:-3.19|P-value:7.06E-4||SGD DESC:Meiosis-specific protein required for chromosome synapsis; displays Red1p dependent localization to the unsynapsed axial-lateral elements of the synaptonemal complex; required for chiasma formation; in vitro, displays the ability to promote intra- and intermolecular synapsis between double-stranded DNA molecules and to fold DNA into rigid protein?DNA filaments Gene:IDP1(YDL066W)|FD-Score:3.91|P-value:4.61E-5||SGD DESC:Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes Gene:IMG1(YCR046C)|FD-Score:-5.99|P-value:1.08E-9||SGD DESC:Mitochondrial ribosomal protein of the large subunit, required for respiration and for maintenance of the mitochondrial genome Gene:IRS4(YKR019C)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LHS1(YKL073W)|FD-Score:3.25|P-value:5.84E-4||SGD DESC:Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway Gene:LYP1(YNL268W)|FD-Score:3.77|P-value:8.24E-5||SGD DESC:Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids Gene:MCH4(YOL119C)|FD-Score:4.59|P-value:2.19E-6||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MLC2(YPR188C)|FD-Score:4.12|P-value:1.88E-5||SGD DESC:Regulatory light chain for the type II myosin, Myo1p; binds to an IQ motif of Myo1p, localization to the bud neck depends on Myo1p; involved in the disassembly of the Myo1p ring Gene:MRPL24(YMR193W)|FD-Score:-4.17|P-value:1.49E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; two mitochondrial ribosomal proteins, YmL14 and YmL24, have been assigned to the same gene Gene:MRPL28(YDR462W)|FD-Score:-4.6|P-value:2.09E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MRPS28(YDR337W)|FD-Score:-5.79|P-value:3.55E-9||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRS4(YKR052C)|FD-Score:-3.8|P-value:7.33E-5||SGD DESC:Iron transporter of the mitochondrial carrier family; mediates Fe2+ transport across the inner mitochondrial membrane; active under low-iron conditions; may transport other cations; protein abundance increases in response to DNA replication stress; MRS4 has a paralog, MRS3, that arose from the whole genome duplication Gene:MSC7(YHR039C)|FD-Score:3.74|P-value:9.31E-5||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids Gene:MYO4(YAL029C)|FD-Score:-3.32|P-value:4.43E-4||SGD DESC:Type V myosin motor involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p; MYO4 has a paralog, MYO2, that arose from the whole genome duplication Gene:PAP2(YOL115W)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Trf5p Gene:PCL9(YDL179W)|FD-Score:-4|P-value:3.11E-5||SGD DESC:Cyclin, forms a functional kinase complex with Pho85p cyclin-dependent kinase (Cdk), expressed in late M/early G1 phase, activated by Swi5p Gene:PDR11(YIL013C)|FD-Score:-3.13|P-value:8.60E-4||SGD DESC:ATP-binding cassette (ABC) transporter, multidrug transporter involved in multiple drug resistance; mediates sterol uptake when sterol biosynthesis is compromised; regulated by Pdr1p; required for anaerobic growth Gene:PER1(YCR044C)|FD-Score:6.75|P-value:7.26E-12||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PGM1(YKL127W)|FD-Score:-3.09|P-value:9.84E-4||SGD DESC:Phosphoglucomutase, minor isoform; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism Gene:PKH1(YDR490C)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh2p Gene:PPH21(YDL134C)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph22p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; forms nuclear foci upon DNA replication stress; PPH21 has a paralog, PPH22, that arose from the whole genome duplication Gene:PPM1(YDR435C)|FD-Score:11.4|P-value:3.10E-30||SGD DESC:Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits Gene:RAD27(YKL113C)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family Gene:RHO2(YNL090W)|FD-Score:-4.07|P-value:2.34E-5||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, involved in the establishment of cell polarity and in microtubule assembly Gene:RKR1(YMR247C)|FD-Score:-3.28|P-value:5.21E-4||SGD DESC:RING domain E3 ubiquitin ligase; involved in the ubiquitin-mediated degradation of non-stop proteins; functional connections to chromatin modification; nuclear protein that also co-localizes with ribosomes; homolog of mouse Listerin, whose mutation has been reported to cause neurodegeneration in mice Gene:RMA1(YKL132C)|FD-Score:4|P-value:3.17E-5||SGD DESC:Putative dihydrofolate synthetase; has similarity to Fol3p and to E. coli folylpolyglutamate synthetase/dihydrofolate synthetase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RMD6(YEL072W)|FD-Score:3.11|P-value:9.33E-4||SGD DESC:Protein required for sporulation Gene:RPL20A(YMR242C)|FD-Score:4.55|P-value:2.70E-6||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RRD1(YIL153W)|FD-Score:4.54|P-value:2.85E-6||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:SAC6(YDR129C)|FD-Score:4.5|P-value:3.42E-6||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SDS3(YIL084C)|FD-Score:4.07|P-value:2.39E-5||SGD DESC:Component of the Rpd3p/Sin3p deacetylase complex required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; cells defective in SDS3 display pleiotropic phenotypes Gene:SLZ1(YNL196C_p)|FD-Score:6.62|P-value:1.76E-11||SGD DESC:Sporulation-specific protein with a leucine zipper motif Gene:SNF8(YPL002C)|FD-Score:5.54|P-value:1.55E-8||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SPS4(YOR313C)|FD-Score:5|P-value:2.84E-7||SGD DESC:Protein whose expression is induced during sporulation; not required for sporulation; heterologous expression in E. coli induces the SOS response that senses DNA damage Gene:TDA3(YHR009C_p)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Putative oxidoreductase involved in late endosome to Golgi transport; physical and genetical interactions with Btn2p; null mutant is viable, has extended S phase, and sensitive to expression of top1-T722A allele; similar to human FOXRED1 Gene:TLG2(YOL018C)|FD-Score:6.79|P-value:5.50E-12||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TVP18(YMR071C)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:VAM3(YOR106W)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region Gene:VAN1(YML115C)|FD-Score:-5.27|P-value:6.83E-8||SGD DESC:Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant Gene:VHC1(YBR235W_p)|FD-Score:-6.35|P-value:1.08E-10||SGD DESC:Vacuolar membrane cation-chloride cotransporter (CCC); likely mediates K+ and Cl- cotransport into the vacuole; has a role in potassium homeostasis and salt tolerance; similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family Gene:VMA13(YPR036W)|FD-Score:4.42|P-value:4.98E-6||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:YBR285W(YBR285W_p)|FD-Score:3.13|P-value:8.72E-4||SGD DESC:Putative protein of unknown function; YBR285W is not an essential gene Gene:YCK3(YER123W)|FD-Score:3.26|P-value:5.65E-4||SGD DESC:Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway Gene:YCR087W(YCR087W_d)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YCR087C-A; YCR087W is not an essential gene Gene:YDL041W(YDL041W_d)|FD-Score:6.96|P-value:1.69E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YDR003W-A(YDR003W-A_p)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YDR008C(YDR008C_d)|FD-Score:-3.8|P-value:7.30E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR203W(YDR203W_d)|FD-Score:3.18|P-value:7.31E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR271C(YDR271C_d)|FD-Score:4.37|P-value:6.26E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W Gene:YDR387C(YDR387C_p)|FD-Score:3.83|P-value:6.50E-5||SGD DESC:Putative transporter, member of the sugar porter family; YDR387C is not an essential gene Gene:YDR535C(YDR535C_d)|FD-Score:3.1|P-value:9.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene. Gene:YER084W(YER084W_p)|FD-Score:4.97|P-value:3.28E-7||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YGR018C(YGR018C_p)|FD-Score:-3.79|P-value:7.51E-5||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the uncharacterized ORF YGR017W Gene:YGR242W(YGR242W_d)|FD-Score:4.19|P-value:1.38E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YAP1802/YGR241C Gene:YHR045W(YHR045W_p)|FD-Score:-3.16|P-value:7.84E-4||SGD DESC:Putative protein of unknown function; possible role in iron metabolism and/or amino acid and carbohydrate metabolism; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum Gene:YKE2(YLR200W)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin Gene:YKR015C(YKR015C_p)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Putative protein of unknown function Gene:YMR209C(YMR209C_p)|FD-Score:4.48|P-value:3.75E-6||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; YMR209C is not an essential gene Gene:YPL014W(YPL014W_p)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus Gene:YPL109C(YPL109C_p)|FD-Score:-4.45|P-value:4.36E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPR012W(YPR012W_d)|FD-Score:5.23|P-value:8.28E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR012W is not an essential gene Gene:YPR170C(YPR170C_d)|FD-Score:5.37|P-value:4.02E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B Gene:YPT35(YHR105W)|FD-Score:-3.69|P-value:1.14E-4||SGD DESC:Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport Gene:YPT6(YLR262C)|FD-Score:3.25|P-value:5.73E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:AAD4(YDL243C)|FD-Score:4.65|P-value:1.66E-6||SGD DESC:Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin Gene:ALF1(YNL148C)|FD-Score:-3.13|P-value:8.77E-4||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:APL6(YGR261C)|FD-Score:3.41|P-value:3.26E-4||SGD DESC:Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools Gene:BEM4(YPL161C)|FD-Score:5.7|P-value:5.89E-9||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BLI1(YKL061W_p)|FD-Score:4.9|P-value:4.86E-7||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome Gene:BNI1(YNL271C)|FD-Score:-3.4|P-value:3.40E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:CAR2(YLR438W)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:L-ornithine transaminase (OTAse); catalyzes the second step of arginine degradation, expression is dually-regulated by allophanate induction and a specific arginine induction process; not nitrogen catabolite repression sensitive; protein abundance increases in response to DNA replication stress Gene:CBP6(YBR120C)|FD-Score:5.11|P-value:1.65E-7||SGD DESC:Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex Gene:COS6(YGR295C)|FD-Score:-3.11|P-value:9.45E-4||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:CSG2(YBR036C)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:CSM1(YCR086W)|FD-Score:4.79|P-value:8.27E-7||SGD DESC:Nucleolar protein that forms a complex with Lrs4p and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:CWC15(YDR163W)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Non-essential protein involved in pre-mRNA splicing, component of a complex containing Cef1p; has similarity to S. pombe Cwf15p Gene:CYB5(YNL111C)|FD-Score:-3.45|P-value:2.76E-4||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:DIA4(YHR011W)|FD-Score:-3.34|P-value:4.23E-4||SGD DESC:Probable mitochondrial seryl-tRNA synthetase, mutant displays increased invasive and pseudohyphal growth Gene:DIN7(YDR263C)|FD-Score:6.11|P-value:4.94E-10||SGD DESC:Mitochondrial nuclease functioning in DNA repair and replication, modulates the stability of the mitochondrial genome, induced by exposure to mutagens, also induced during meiosis at a time nearly coincident with commitment to recombination Gene:DMA1(YHR115C)|FD-Score:-3.38|P-value:3.63E-4||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma2p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8 protein, similar to human Chfr; contains FHA and RING finger Gene:FAR3(YMR052W)|FD-Score:6.14|P-value:4.09E-10||SGD DESC:Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:FMO1(YHR176W)|FD-Score:4.53|P-value:2.99E-6||SGD DESC:Flavin-containing monooxygenase, localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins Gene:FMP40(YPL222W_p)|FD-Score:3.2|P-value:6.96E-4||SGD DESC:Putative protein of unknown function; proposed to be involved in responding to environmental stresses; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GCN2(YDR283C)|FD-Score:-3.35|P-value:4.11E-4||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCV1(YDR019C)|FD-Score:5.24|P-value:7.86E-8||SGD DESC:T subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm Gene:GEF1(YJR040W)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism Gene:GEP7(YGL057C)|FD-Score:4.42|P-value:4.98E-6||SGD DESC:Protein of unknown function; null mutant exhibits a respiratory growth defect and synthetic interactions with prohibitin (phb1) and gem1; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:HBT1(YDL223C)|FD-Score:4.28|P-value:9.29E-6||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:HOM6(YJR139C)|FD-Score:-3.62|P-value:1.47E-4||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:HOP1(YIL072W)|FD-Score:-3.19|P-value:7.06E-4||SGD DESC:Meiosis-specific protein required for chromosome synapsis; displays Red1p dependent localization to the unsynapsed axial-lateral elements of the synaptonemal complex; required for chiasma formation; in vitro, displays the ability to promote intra- and intermolecular synapsis between double-stranded DNA molecules and to fold DNA into rigid protein?DNA filaments Gene:IDP1(YDL066W)|FD-Score:3.91|P-value:4.61E-5||SGD DESC:Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes Gene:IMG1(YCR046C)|FD-Score:-5.99|P-value:1.08E-9||SGD DESC:Mitochondrial ribosomal protein of the large subunit, required for respiration and for maintenance of the mitochondrial genome Gene:IRS4(YKR019C)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LHS1(YKL073W)|FD-Score:3.25|P-value:5.84E-4||SGD DESC:Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway Gene:LYP1(YNL268W)|FD-Score:3.77|P-value:8.24E-5||SGD DESC:Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids Gene:MCH4(YOL119C)|FD-Score:4.59|P-value:2.19E-6||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MLC2(YPR188C)|FD-Score:4.12|P-value:1.88E-5||SGD DESC:Regulatory light chain for the type II myosin, Myo1p; binds to an IQ motif of Myo1p, localization to the bud neck depends on Myo1p; involved in the disassembly of the Myo1p ring Gene:MRPL24(YMR193W)|FD-Score:-4.17|P-value:1.49E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; two mitochondrial ribosomal proteins, YmL14 and YmL24, have been assigned to the same gene Gene:MRPL28(YDR462W)|FD-Score:-4.6|P-value:2.09E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MRPS28(YDR337W)|FD-Score:-5.79|P-value:3.55E-9||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRS4(YKR052C)|FD-Score:-3.8|P-value:7.33E-5||SGD DESC:Iron transporter of the mitochondrial carrier family; mediates Fe2+ transport across the inner mitochondrial membrane; active under low-iron conditions; may transport other cations; protein abundance increases in response to DNA replication stress; MRS4 has a paralog, MRS3, that arose from the whole genome duplication Gene:MSC7(YHR039C)|FD-Score:3.74|P-value:9.31E-5||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids Gene:MYO4(YAL029C)|FD-Score:-3.32|P-value:4.43E-4||SGD DESC:Type V myosin motor involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p; MYO4 has a paralog, MYO2, that arose from the whole genome duplication Gene:PAP2(YOL115W)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Trf5p Gene:PCL9(YDL179W)|FD-Score:-4|P-value:3.11E-5||SGD DESC:Cyclin, forms a functional kinase complex with Pho85p cyclin-dependent kinase (Cdk), expressed in late M/early G1 phase, activated by Swi5p Gene:PDR11(YIL013C)|FD-Score:-3.13|P-value:8.60E-4||SGD DESC:ATP-binding cassette (ABC) transporter, multidrug transporter involved in multiple drug resistance; mediates sterol uptake when sterol biosynthesis is compromised; regulated by Pdr1p; required for anaerobic growth Gene:PER1(YCR044C)|FD-Score:6.75|P-value:7.26E-12||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PGM1(YKL127W)|FD-Score:-3.09|P-value:9.84E-4||SGD DESC:Phosphoglucomutase, minor isoform; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism Gene:PKH1(YDR490C)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh2p Gene:PPH21(YDL134C)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph22p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; forms nuclear foci upon DNA replication stress; PPH21 has a paralog, PPH22, that arose from the whole genome duplication Gene:PPM1(YDR435C)|FD-Score:11.4|P-value:3.10E-30||SGD DESC:Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits Gene:RAD27(YKL113C)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family Gene:RHO2(YNL090W)|FD-Score:-4.07|P-value:2.34E-5||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, involved in the establishment of cell polarity and in microtubule assembly Gene:RKR1(YMR247C)|FD-Score:-3.28|P-value:5.21E-4||SGD DESC:RING domain E3 ubiquitin ligase; involved in the ubiquitin-mediated degradation of non-stop proteins; functional connections to chromatin modification; nuclear protein that also co-localizes with ribosomes; homolog of mouse Listerin, whose mutation has been reported to cause neurodegeneration in mice Gene:RMA1(YKL132C)|FD-Score:4|P-value:3.17E-5||SGD DESC:Putative dihydrofolate synthetase; has similarity to Fol3p and to E. coli folylpolyglutamate synthetase/dihydrofolate synthetase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RMD6(YEL072W)|FD-Score:3.11|P-value:9.33E-4||SGD DESC:Protein required for sporulation Gene:RPL20A(YMR242C)|FD-Score:4.55|P-value:2.70E-6||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RRD1(YIL153W)|FD-Score:4.54|P-value:2.85E-6||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:SAC6(YDR129C)|FD-Score:4.5|P-value:3.42E-6||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SDS3(YIL084C)|FD-Score:4.07|P-value:2.39E-5||SGD DESC:Component of the Rpd3p/Sin3p deacetylase complex required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; cells defective in SDS3 display pleiotropic phenotypes Gene:SLZ1(YNL196C_p)|FD-Score:6.62|P-value:1.76E-11||SGD DESC:Sporulation-specific protein with a leucine zipper motif Gene:SNF8(YPL002C)|FD-Score:5.54|P-value:1.55E-8||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SPS4(YOR313C)|FD-Score:5|P-value:2.84E-7||SGD DESC:Protein whose expression is induced during sporulation; not required for sporulation; heterologous expression in E. coli induces the SOS response that senses DNA damage Gene:TDA3(YHR009C_p)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Putative oxidoreductase involved in late endosome to Golgi transport; physical and genetical interactions with Btn2p; null mutant is viable, has extended S phase, and sensitive to expression of top1-T722A allele; similar to human FOXRED1 Gene:TLG2(YOL018C)|FD-Score:6.79|P-value:5.50E-12||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TVP18(YMR071C)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:VAM3(YOR106W)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region Gene:VAN1(YML115C)|FD-Score:-5.27|P-value:6.83E-8||SGD DESC:Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant Gene:VHC1(YBR235W_p)|FD-Score:-6.35|P-value:1.08E-10||SGD DESC:Vacuolar membrane cation-chloride cotransporter (CCC); likely mediates K+ and Cl- cotransport into the vacuole; has a role in potassium homeostasis and salt tolerance; similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family Gene:VMA13(YPR036W)|FD-Score:4.42|P-value:4.98E-6||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:YBR285W(YBR285W_p)|FD-Score:3.13|P-value:8.72E-4||SGD DESC:Putative protein of unknown function; YBR285W is not an essential gene Gene:YCK3(YER123W)|FD-Score:3.26|P-value:5.65E-4||SGD DESC:Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway Gene:YCR087W(YCR087W_d)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YCR087C-A; YCR087W is not an essential gene Gene:YDL041W(YDL041W_d)|FD-Score:6.96|P-value:1.69E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YDR003W-A(YDR003W-A_p)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YDR008C(YDR008C_d)|FD-Score:-3.8|P-value:7.30E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR203W(YDR203W_d)|FD-Score:3.18|P-value:7.31E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR271C(YDR271C_d)|FD-Score:4.37|P-value:6.26E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W Gene:YDR387C(YDR387C_p)|FD-Score:3.83|P-value:6.50E-5||SGD DESC:Putative transporter, member of the sugar porter family; YDR387C is not an essential gene Gene:YDR535C(YDR535C_d)|FD-Score:3.1|P-value:9.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene. Gene:YER084W(YER084W_p)|FD-Score:4.97|P-value:3.28E-7||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YGR018C(YGR018C_p)|FD-Score:-3.79|P-value:7.51E-5||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the uncharacterized ORF YGR017W Gene:YGR242W(YGR242W_d)|FD-Score:4.19|P-value:1.38E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YAP1802/YGR241C Gene:YHR045W(YHR045W_p)|FD-Score:-3.16|P-value:7.84E-4||SGD DESC:Putative protein of unknown function; possible role in iron metabolism and/or amino acid and carbohydrate metabolism; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum Gene:YKE2(YLR200W)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin Gene:YKR015C(YKR015C_p)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Putative protein of unknown function Gene:YMR209C(YMR209C_p)|FD-Score:4.48|P-value:3.75E-6||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; YMR209C is not an essential gene Gene:YPL014W(YPL014W_p)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus Gene:YPL109C(YPL109C_p)|FD-Score:-4.45|P-value:4.36E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPR012W(YPR012W_d)|FD-Score:5.23|P-value:8.28E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR012W is not an essential gene Gene:YPR170C(YPR170C_d)|FD-Score:5.37|P-value:4.02E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B Gene:YPT35(YHR105W)|FD-Score:-3.69|P-value:1.14E-4||SGD DESC:Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport Gene:YPT6(YLR262C)|FD-Score:3.25|P-value:5.73E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPL016W8.672.23E-181.87SWI1Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer
YDR526C_d7.687.81E-151.87YDR526C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YKL173W5.813.11E-90.39SNU114GTPase component of U5 snRNP involved in mRNA splicing via spliceosome; binds directly to U5 snRNA; proposed to be involved in conformational changes of the spliceosome; similarity to ribosomal translocation factor EF-2
YDL195W5.422.90E-80.18SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YDR361C5.257.69E-80.63BCP1Essential protein involved in nuclear export of Mss4p, which is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport
YGL065C4.621.90E-61.14ALG2Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol
YOR340C3.492.46E-40.06RPA43RNA polymerase I subunit A43
YMR211W3.423.08E-40.10DML1Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
YDR081C3.324.46E-40.07PDC2Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions
YDR376W3.255.72E-40.08ARH1Oxidoreductase of the mitochondrial inner membrane, involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viability
YNL062C3.177.63E-40.03GCD10Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression
YOR074C3.148.54E-40.04CDC21Thymidylate synthase, required for de novo biosynthesis of pyrimidine deoxyribonucleotides; expression is induced at G1/S
YDR373W3.109.74E-40.02FRQ1N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily
YOR319W3.080.001040.05HSH49U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM)
YPR103W3.030.001220.02PRE2Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR435C11.403.10E-30PPM1Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits
YDL041W_d6.961.69E-12YDL041W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C
YOL018C6.795.50E-12TLG2Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16)
YCR044C6.757.26E-12PER1Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1
YNL196C_p6.621.76E-11SLZ1_pSporulation-specific protein with a leucine zipper motif
YMR052W6.144.09E-10FAR3Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress
YDR263C6.114.94E-10DIN7Mitochondrial nuclease functioning in DNA repair and replication, modulates the stability of the mitochondrial genome, induced by exposure to mutagens, also induced during meiosis at a time nearly coincident with commitment to recombination
YPL161C5.705.89E-9BEM4Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length
YPL002C5.541.55E-8SNF8Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression
YPR170C_d5.374.02E-8YPR170C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B
YDR019C5.247.86E-8GCV1T subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm
YPR012W_d5.238.28E-8YPR012W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR012W is not an essential gene
YBR120C5.111.65E-7CBP6Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
YOR313C5.002.84E-7SPS4Protein whose expression is induced during sporulation; not required for sporulation; heterologous expression in E. coli induces the SOS response that senses DNA damage
YER084W_p4.973.28E-7YER084W_pProtein of unknown function; expressed at both mRNA and protein levels

GO enrichment analysis for SGTC_2824
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1661.20E-37SGTC_28679039098 71.4 μMChembridge (Drug-like library)64634600.363636iron homeostasis
0.1497.09E-31SGTC_28187996756 13.0 μMChembridge (Drug-like library)22416210.227273
0.1255.93E-22SGTC_2666tannic acid 100.0 μMMicrosource (Natural product library)161342670.0958904iron homeostasis
0.1218.59E-21SGTC_1026k007-1105 30.6 μMChemDiv (Drug-like library)70667940.163934ERAD & cell cycle
0.1095.44E-17SGTC_31899110682 49.5 μMChembridge (Drug-like library)171224180.288136
0.1032.77E-15SGTC_3393091-4833 71.4 μMChemDiv (Drug-like library)32400770.111111iron homeostasis
0.1001.49E-14SGTC_28177995342 71.4 μMChembridge (Drug-like library)188799750.117647
0.0992.86E-14SGTC_28227994718 71.4 μMChembridge (Drug-like library)10180110.157143
0.0984.87E-14SGTC_8940960-0238 55.9 μMChemDiv (Drug-like library)67419070.238806
0.0976.11E-14SGTC_1994st072947 40.9 μMTimTec (Natural product derivative library)52865650.08
0.0944.49E-13SGTC_27847567673 72.7 μMChembridge (Drug-like library)34712470.0746269iron homeostasis
0.0921.50E-12SGTC_30199081971 49.5 μMChembridge (Drug-like library)164518030.0675676
0.0912.51E-12SGTC_1724st037281 8.3 μMTimTec (Natural product derivative library)5741240.193548
0.0913.10E-12SGTC_5914299-0174 154.0 μMChemDiv (Drug-like library)55090880.0704225
0.0896.53E-12SGTC_2675theaflavin digallate 100.0 μMMicrosource (Natural product library)4673200.0813954iron homeostasis

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_20134024628200 μM0.655230760Chembridge (Fragment library)226.273762.35422
SGTC_21826325192200 μM0.585366736248Chembridge (Fragment library)226.273762.35422Golgi
SGTC_3129912725749.47 μM0.5837894Chembridge (Drug-like library)249.371763.61812
SGTC_21935925765200 μM0.468085877370Chembridge (Fragment library)241.28512.49622
SGTC_3004907612271.43 μM0.46153844782551Chembridge (Drug-like library)267.322383.53612
SGTC_3109912285349.47 μM0.461234709Chembridge (Drug-like library)287.376683.17413
SGTC_3232913287449.47 μM0.4444443784940Chembridge (Drug-like library)255.268622.4713RPP1 & pyrimidine depletion
SGTC_5544483-2211187 μM0.4375747107ChemDiv (Drug-like library)285.29462.45314
SGTC_21505556613200 μM0.4375743190Chembridge (Fragment library)213.235241.22813
SGTC_2900532852885.29 μM0.4375795359Chembridge (Drug-like library)261.703583.26212Golgi